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  • 92
    Difco s typhimurium
    Histopathology of Peyer’s patches and ileum from perinatal calves inoculated per os with 10 10 CFU of the indicated strains of S. <t>typhimurium</t> per animal, photographed at a 40× magnification. Shown are hematoxylin-and-eosin-stained sections of Peyer’s patch (left column) and terminal ileum (right column). Short arrows indicate areas of marked lymphoid depletion; long arrows indicate zones of variable degrees of fibrinopurulent necrotizing ileitis at the mucosal surface.
    S Typhimurium, supplied by Difco, used in various techniques. Bioz Stars score: 92/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s typhimurium/product/Difco
    Average 92 stars, based on 100 article reviews
    Price from $9.99 to $1999.99
    s typhimurium - by Bioz Stars, 2020-08
    92/100 stars
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    92
    Difco salmonella typhimurium
    Schematic molecular model of S. <t>Typhimurium</t> cell wall structure. The Salmonella cell wall comprises the inner membrane, the periplasmic space filled with a gel-like matrix and the outer membrane. The inner membrane is the innermost component, whereas the outer membrane is the outermost impermeable structure, which is a bilayer consisting of a phospholipid layer on the inner side, and a lipopolysaccharide layer towards the outer side, as well as lipoproteins anchored into the membrane. Deletion of the specific gene responsible for lipopolysaccharide (LPS) synthesis would lead to truncation of LPS, thus resulting in the membrane rearrangement and altering the amounts and categories of lipoproteins. The figure represents the types of truncated LPS in this study. (Kdo, 3-deoxy- d -mannooctulosonic acid; PPEtN, pyrophosphorylethanolamine; Hep, heptose; GlcNAc, N -acetylglucosamine; Glc, glucose; Gal, galactose; P, phosphate). Deletion of waaC encoding heptosyltransferase I leads to a deep rough LPS structure carrying only Kdo-lipid A (Re); deletion of waaF encoding heptosyltransferase II results in a mutant generating a LPS structure with lipid A and a truncated inner core (Rd2); deletion of waaG encoding a glycosyltransferase leads to the Rd1 type of LPS lacking the outer core, and consequently, waaI and waaJ mutants produce the truncated core phenotype of Rb3 and Rb2, respectively; deletion of both waaL encoding O -antigen ligase and wbaP encoding Und-P galactose phosphotransferase leads to the production of core-lipid A (Ra) in the mutants; wzy encoding O -antigen polymerase is one of three processing genes, the deletion of which results in a mutant generating a semi-rough LPS carrying a single O -antigen unit with a complete lipid A core, and the deletion of rfaH encoding the transcriptional antiterminator leads to the production of the truncated core phenotype (Rb3).
    Salmonella Typhimurium, supplied by Difco, used in various techniques. Bioz Stars score: 92/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/salmonella typhimurium/product/Difco
    Average 92 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    salmonella typhimurium - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    Image Search Results


    Histopathology of Peyer’s patches and ileum from perinatal calves inoculated per os with 10 10 CFU of the indicated strains of S. typhimurium per animal, photographed at a 40× magnification. Shown are hematoxylin-and-eosin-stained sections of Peyer’s patch (left column) and terminal ileum (right column). Short arrows indicate areas of marked lymphoid depletion; long arrows indicate zones of variable degrees of fibrinopurulent necrotizing ileitis at the mucosal surface.

    Journal: Infection and Immunity

    Article Title: Contribution of Salmonella typhimurium Virulence Factors to Diarrheal Disease in Calves

    doi:

    Figure Lengend Snippet: Histopathology of Peyer’s patches and ileum from perinatal calves inoculated per os with 10 10 CFU of the indicated strains of S. typhimurium per animal, photographed at a 40× magnification. Shown are hematoxylin-and-eosin-stained sections of Peyer’s patch (left column) and terminal ileum (right column). Short arrows indicate areas of marked lymphoid depletion; long arrows indicate zones of variable degrees of fibrinopurulent necrotizing ileitis at the mucosal surface.

    Article Snippet: Shedding of S. typhimurium was monitored by taking daily fecal swabs, subsequently enriching them in tetrathionate broth (Difco), and plating them on brilliant green agar (BBL).

    Techniques: Histopathology, Staining

    Representative examples of the gross pathology of Peyer’s patch and mucosa of the terminal ileum of calves inoculated orally with 10 10 CFU of different S. typhimurium strains. (A) Severe acute fibrinopurulent necrotizing enteritis with segmental or continuous pseudomembrane formation of calves inoculated with wild type (IR715), strain STN272 ( spvR ), or strain SVM255 ( sigD ). (B) Moderate to marked subacute fibrinopurulent enteritis often confined to Peyer’s patches of calves inoculated with STN119 ( spiB ) or STN166 ( rfaJ ). (C) Normal Peyer’s patch and ileal mucosa of calves inoculated with STN61 ( hilA ), STN162 ( prgH ), or CL1509 ( aroA ) or of uninoculated controls. Bar = 1 cm.

    Journal: Infection and Immunity

    Article Title: Contribution of Salmonella typhimurium Virulence Factors to Diarrheal Disease in Calves

    doi:

    Figure Lengend Snippet: Representative examples of the gross pathology of Peyer’s patch and mucosa of the terminal ileum of calves inoculated orally with 10 10 CFU of different S. typhimurium strains. (A) Severe acute fibrinopurulent necrotizing enteritis with segmental or continuous pseudomembrane formation of calves inoculated with wild type (IR715), strain STN272 ( spvR ), or strain SVM255 ( sigD ). (B) Moderate to marked subacute fibrinopurulent enteritis often confined to Peyer’s patches of calves inoculated with STN119 ( spiB ) or STN166 ( rfaJ ). (C) Normal Peyer’s patch and ileal mucosa of calves inoculated with STN61 ( hilA ), STN162 ( prgH ), or CL1509 ( aroA ) or of uninoculated controls. Bar = 1 cm.

    Article Snippet: Shedding of S. typhimurium was monitored by taking daily fecal swabs, subsequently enriching them in tetrathionate broth (Difco), and plating them on brilliant green agar (BBL).

    Techniques:

    Schematic molecular model of S. Typhimurium cell wall structure. The Salmonella cell wall comprises the inner membrane, the periplasmic space filled with a gel-like matrix and the outer membrane. The inner membrane is the innermost component, whereas the outer membrane is the outermost impermeable structure, which is a bilayer consisting of a phospholipid layer on the inner side, and a lipopolysaccharide layer towards the outer side, as well as lipoproteins anchored into the membrane. Deletion of the specific gene responsible for lipopolysaccharide (LPS) synthesis would lead to truncation of LPS, thus resulting in the membrane rearrangement and altering the amounts and categories of lipoproteins. The figure represents the types of truncated LPS in this study. (Kdo, 3-deoxy- d -mannooctulosonic acid; PPEtN, pyrophosphorylethanolamine; Hep, heptose; GlcNAc, N -acetylglucosamine; Glc, glucose; Gal, galactose; P, phosphate). Deletion of waaC encoding heptosyltransferase I leads to a deep rough LPS structure carrying only Kdo-lipid A (Re); deletion of waaF encoding heptosyltransferase II results in a mutant generating a LPS structure with lipid A and a truncated inner core (Rd2); deletion of waaG encoding a glycosyltransferase leads to the Rd1 type of LPS lacking the outer core, and consequently, waaI and waaJ mutants produce the truncated core phenotype of Rb3 and Rb2, respectively; deletion of both waaL encoding O -antigen ligase and wbaP encoding Und-P galactose phosphotransferase leads to the production of core-lipid A (Ra) in the mutants; wzy encoding O -antigen polymerase is one of three processing genes, the deletion of which results in a mutant generating a semi-rough LPS carrying a single O -antigen unit with a complete lipid A core, and the deletion of rfaH encoding the transcriptional antiterminator leads to the production of the truncated core phenotype (Rb3).

    Journal: International Journal of Molecular Sciences

    Article Title: Immunogenicity and Cross-Protective Efficacy Induced by Outer Membrane Proteins from Salmonella Typhimurium Mutants with Truncated LPS in Mice

    doi: 10.3390/ijms17030416

    Figure Lengend Snippet: Schematic molecular model of S. Typhimurium cell wall structure. The Salmonella cell wall comprises the inner membrane, the periplasmic space filled with a gel-like matrix and the outer membrane. The inner membrane is the innermost component, whereas the outer membrane is the outermost impermeable structure, which is a bilayer consisting of a phospholipid layer on the inner side, and a lipopolysaccharide layer towards the outer side, as well as lipoproteins anchored into the membrane. Deletion of the specific gene responsible for lipopolysaccharide (LPS) synthesis would lead to truncation of LPS, thus resulting in the membrane rearrangement and altering the amounts and categories of lipoproteins. The figure represents the types of truncated LPS in this study. (Kdo, 3-deoxy- d -mannooctulosonic acid; PPEtN, pyrophosphorylethanolamine; Hep, heptose; GlcNAc, N -acetylglucosamine; Glc, glucose; Gal, galactose; P, phosphate). Deletion of waaC encoding heptosyltransferase I leads to a deep rough LPS structure carrying only Kdo-lipid A (Re); deletion of waaF encoding heptosyltransferase II results in a mutant generating a LPS structure with lipid A and a truncated inner core (Rd2); deletion of waaG encoding a glycosyltransferase leads to the Rd1 type of LPS lacking the outer core, and consequently, waaI and waaJ mutants produce the truncated core phenotype of Rb3 and Rb2, respectively; deletion of both waaL encoding O -antigen ligase and wbaP encoding Und-P galactose phosphotransferase leads to the production of core-lipid A (Ra) in the mutants; wzy encoding O -antigen polymerase is one of three processing genes, the deletion of which results in a mutant generating a semi-rough LPS carrying a single O -antigen unit with a complete lipid A core, and the deletion of rfaH encoding the transcriptional antiterminator leads to the production of the truncated core phenotype (Rb3).

    Article Snippet: Salmonella Typhimurium (S. Typhimurium) and Escherichia coli (E . coli ) were grown in Luria–Bertani (LB) [ ] broth or agar or nutrient broth (Difco, Detroit, MI, USA) at 37 °C.

    Techniques: Gas Chromatography, Mutagenesis

    Competitive ELISA to confirm the cross-reactivity of OMPs from the truncated LPS mutants against heterologous Salmonella . OMPs isolated from S. Typhimurium were incubated in the plate as the coating antigen. OMPs from S. Choleraesuis ( A ) or S. Enteritidis ( B ) as the competitive antigen and OMPs from S. Typhimurium ( C ) as the control competitive antigen diluted from 1/10 to 1/7290 (Dilution, 1/X) were incubated in wells. The sera were obtained from mice ( n = 8) immunized with OMPs from the parent strain and its mutants at eight weeks after the primary immunization. The error bars represent variations from triplicate wells. *** p

    Journal: International Journal of Molecular Sciences

    Article Title: Immunogenicity and Cross-Protective Efficacy Induced by Outer Membrane Proteins from Salmonella Typhimurium Mutants with Truncated LPS in Mice

    doi: 10.3390/ijms17030416

    Figure Lengend Snippet: Competitive ELISA to confirm the cross-reactivity of OMPs from the truncated LPS mutants against heterologous Salmonella . OMPs isolated from S. Typhimurium were incubated in the plate as the coating antigen. OMPs from S. Choleraesuis ( A ) or S. Enteritidis ( B ) as the competitive antigen and OMPs from S. Typhimurium ( C ) as the control competitive antigen diluted from 1/10 to 1/7290 (Dilution, 1/X) were incubated in wells. The sera were obtained from mice ( n = 8) immunized with OMPs from the parent strain and its mutants at eight weeks after the primary immunization. The error bars represent variations from triplicate wells. *** p

    Article Snippet: Salmonella Typhimurium (S. Typhimurium) and Escherichia coli (E . coli ) were grown in Luria–Bertani (LB) [ ] broth or agar or nutrient broth (Difco, Detroit, MI, USA) at 37 °C.

    Techniques: Competitive ELISA, Isolation, Incubation, Mouse Assay

    Serum antibody responses in mice immunized by the intranasal route. Total serum IgG specific for OMPs from S. Typhimurium ( A ), IgG1 specific for OMPs from S. Typhimurium ( B ) and IgG2a specific for OMPs from S. Typhimurium ( C ) were measured by enzyme-linked immunosorbent assay (ELISA). Each group has eight mice. The data represented the reciprocal anti-IgG antibody titer in pooled sera from mice immunized with OMPs from parent strain S100 and its mutants with truncated core or O -antigen at the indicated weeks after immunization. The error bars represented variations between triplicate wells. The mice were boosted at Week 5. *** p

    Journal: International Journal of Molecular Sciences

    Article Title: Immunogenicity and Cross-Protective Efficacy Induced by Outer Membrane Proteins from Salmonella Typhimurium Mutants with Truncated LPS in Mice

    doi: 10.3390/ijms17030416

    Figure Lengend Snippet: Serum antibody responses in mice immunized by the intranasal route. Total serum IgG specific for OMPs from S. Typhimurium ( A ), IgG1 specific for OMPs from S. Typhimurium ( B ) and IgG2a specific for OMPs from S. Typhimurium ( C ) were measured by enzyme-linked immunosorbent assay (ELISA). Each group has eight mice. The data represented the reciprocal anti-IgG antibody titer in pooled sera from mice immunized with OMPs from parent strain S100 and its mutants with truncated core or O -antigen at the indicated weeks after immunization. The error bars represented variations between triplicate wells. The mice were boosted at Week 5. *** p

    Article Snippet: Salmonella Typhimurium (S. Typhimurium) and Escherichia coli (E . coli ) were grown in Luria–Bertani (LB) [ ] broth or agar or nutrient broth (Difco, Detroit, MI, USA) at 37 °C.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay