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  • 97
    ATCC s mitis
    S Mitis, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 152 article reviews
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    94
    ATCC s mitis atcc 6249
    Effect of 3 mM HEMA concentration on sessile (A) and planktonic (B) growth phases of Streptococcus mitis DS12 ( Sm DS12) and S. mitis <t>ATCC</t> 6249 ( Sm ATCC) alone and co-cultured on human gingival fibroblasts after 48 and 72 h of treatment.
    S Mitis Atcc 6249, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 105 article reviews
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    92
    ATCC s mitis atcc 33399
    Inhibition of oral streptococcal species by  S. mutans  UA140. 1,  S. gordonii ; 2,  S. pyogenes ; 3,  S. oralis ; 4,  S. mitis  ATCC 33399; 5,  S. mitis  ATCC 903; 6,  S. pneumoniae ; 7,  S. cristatus ; 8,  S. parasanguinis ; 9,  S. sanguinis  ATCC 10556; 10,  S. sanguinis
    S Mitis Atcc 33399, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 12 article reviews
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    92/100 stars
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    93
    ATCC s mitis atcc 15912
    Inhibition of oral streptococcal species by  S. mutans  UA140. 1,  S. gordonii ; 2,  S. pyogenes ; 3,  S. oralis ; 4,  S. mitis  ATCC 33399; 5,  S. mitis  ATCC 903; 6,  S. pneumoniae ; 7,  S. cristatus ; 8,  S. parasanguinis ; 9,  S. sanguinis  ATCC 10556; 10,  S. sanguinis
    S Mitis Atcc 15912, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 17 article reviews
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    91
    ATCC s mitis atcc 15914
    Inhibition of oral streptococcal species by  S. mutans  UA140. 1,  S. gordonii ; 2,  S. pyogenes ; 3,  S. oralis ; 4,  S. mitis  ATCC 33399; 5,  S. mitis  ATCC 903; 6,  S. pneumoniae ; 7,  S. cristatus ; 8,  S. parasanguinis ; 9,  S. sanguinis  ATCC 10556; 10,  S. sanguinis
    S Mitis Atcc 15914, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 10 article reviews
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    90
    Corning Life Sciences s mitis
    Inhibition of oral streptococcal species by  S. mutans  UA140. 1,  S. gordonii ; 2,  S. pyogenes ; 3,  S. oralis ; 4,  S. mitis  ATCC 33399; 5,  S. mitis  ATCC 903; 6,  S. pneumoniae ; 7,  S. cristatus ; 8,  S. parasanguinis ; 9,  S. sanguinis  ATCC 10556; 10,  S. sanguinis
    S Mitis, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 10 article reviews
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    93
    ATCC strain s mitis ds12
    (a) Light phase-contrast microscopy images of co-cultured human gingival fibroblasts (HGF) cells in different experimental conditions: HGF, HGF-HEMA, HGF/ Sm <t>DS12,</t> HGF-HEMA/ Sm DS12, HGF/ Sm ATCC and HGF-HEMA/ Sm ATCC after 48 h (left panel) and 72 h (right panel) of treatment. Arrows indicate dead cells. Magnification 10×. (b) Graphic representation of cell death percentage assessed by trypan blue dye exclusion test, after 48 and 72 h of treatment. Data are the mean (±SD) of three different consistent experiments. *48 h HGF % dead cells versus 48 h HGF-HEMA % dead cells P
    Strain S Mitis Ds12, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    ATCC s mitis atcc 15909
    (a) Light phase-contrast microscopy images of co-cultured human gingival fibroblasts (HGF) cells in different experimental conditions: HGF, HGF-HEMA, HGF/ Sm <t>DS12,</t> HGF-HEMA/ Sm DS12, HGF/ Sm ATCC and HGF-HEMA/ Sm ATCC after 48 h (left panel) and 72 h (right panel) of treatment. Arrows indicate dead cells. Magnification 10×. (b) Graphic representation of cell death percentage assessed by trypan blue dye exclusion test, after 48 and 72 h of treatment. Data are the mean (±SD) of three different consistent experiments. *48 h HGF % dead cells versus 48 h HGF-HEMA % dead cells P
    S Mitis Atcc 15909, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    ATCC s mitis atcc 9811
    (a) Light phase-contrast microscopy images of co-cultured human gingival fibroblasts (HGF) cells in different experimental conditions: HGF, HGF-HEMA, HGF/ Sm <t>DS12,</t> HGF-HEMA/ Sm DS12, HGF/ Sm ATCC and HGF-HEMA/ Sm ATCC after 48 h (left panel) and 72 h (right panel) of treatment. Arrows indicate dead cells. Magnification 10×. (b) Graphic representation of cell death percentage assessed by trypan blue dye exclusion test, after 48 and 72 h of treatment. Data are the mean (±SD) of three different consistent experiments. *48 h HGF % dead cells versus 48 h HGF-HEMA % dead cells P
    S Mitis Atcc 9811, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    GenScript s mitis ccug31611t
    (a) Light phase-contrast microscopy images of co-cultured human gingival fibroblasts (HGF) cells in different experimental conditions: HGF, HGF-HEMA, HGF/ Sm <t>DS12,</t> HGF-HEMA/ Sm DS12, HGF/ Sm ATCC and HGF-HEMA/ Sm ATCC after 48 h (left panel) and 72 h (right panel) of treatment. Arrows indicate dead cells. Magnification 10×. (b) Graphic representation of cell death percentage assessed by trypan blue dye exclusion test, after 48 and 72 h of treatment. Data are the mean (±SD) of three different consistent experiments. *48 h HGF % dead cells versus 48 h HGF-HEMA % dead cells P
    S Mitis Ccug31611t, supplied by GenScript, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effect of 3 mM HEMA concentration on sessile (A) and planktonic (B) growth phases of Streptococcus mitis DS12 ( Sm DS12) and S. mitis ATCC 6249 ( Sm ATCC) alone and co-cultured on human gingival fibroblasts after 48 and 72 h of treatment.

    Journal: Apmis

    Article Title: Streptococcus mitis/human gingival fibroblasts co-culture: the best natural association in answer to the 2-hydroxyethyl methacrylate release

    doi: 10.1111/j.1600-0463.2011.02828.x

    Figure Lengend Snippet: Effect of 3 mM HEMA concentration on sessile (A) and planktonic (B) growth phases of Streptococcus mitis DS12 ( Sm DS12) and S. mitis ATCC 6249 ( Sm ATCC) alone and co-cultured on human gingival fibroblasts after 48 and 72 h of treatment.

    Article Snippet: Probably, a clear relationship was established between HGFs and S. mitis ATCC 6249 that attempted to modulate the interactions with HEMA to benefit each other.

    Techniques: Concentration Assay, Cell Culture

    Effect of 3 mM 2-hydroxyethyl methacrylate (HEMA) concentration on the viability of Streptococcus mitis DS12 ( Sm DS12) and S. mitis ATCC 6249 ( Sm ATCC) alone and co-cultured on human gingival fibroblasts (HGFs) after 48 and 72 h of treatment. (A) Representative images of sessile streptococcal strains alone (rows 1 and 3) and co-cultured with HGFs (rows 2 and 4) untreated and treated with HEMA. Arrows indicate the bacterial aggregation on HGFs. Images showed in inserts represent the corresponding planktonic phases in which the HEMA treatment produces a significant increase of bacterial viability with respect to the control (see histograms; B). Live/dead staining, scale bar = 10 μm; (B) percentage of streptococcal viability on sessile and planktonic growth phases, untreated and treated with HEMA.

    Journal: Apmis

    Article Title: Streptococcus mitis/human gingival fibroblasts co-culture: the best natural association in answer to the 2-hydroxyethyl methacrylate release

    doi: 10.1111/j.1600-0463.2011.02828.x

    Figure Lengend Snippet: Effect of 3 mM 2-hydroxyethyl methacrylate (HEMA) concentration on the viability of Streptococcus mitis DS12 ( Sm DS12) and S. mitis ATCC 6249 ( Sm ATCC) alone and co-cultured on human gingival fibroblasts (HGFs) after 48 and 72 h of treatment. (A) Representative images of sessile streptococcal strains alone (rows 1 and 3) and co-cultured with HGFs (rows 2 and 4) untreated and treated with HEMA. Arrows indicate the bacterial aggregation on HGFs. Images showed in inserts represent the corresponding planktonic phases in which the HEMA treatment produces a significant increase of bacterial viability with respect to the control (see histograms; B). Live/dead staining, scale bar = 10 μm; (B) percentage of streptococcal viability on sessile and planktonic growth phases, untreated and treated with HEMA.

    Article Snippet: Probably, a clear relationship was established between HGFs and S. mitis ATCC 6249 that attempted to modulate the interactions with HEMA to benefit each other.

    Techniques: Concentration Assay, Cell Culture, Staining

    Inhibition of oral streptococcal species by  S. mutans  UA140. 1,  S. gordonii ; 2,  S. pyogenes ; 3,  S. oralis ; 4,  S. mitis  ATCC 33399; 5,  S. mitis  ATCC 903; 6,  S. pneumoniae ; 7,  S. cristatus ; 8,  S. parasanguinis ; 9,  S. sanguinis  ATCC 10556; 10,  S. sanguinis

    Journal:

    Article Title: Competition and Coexistence between Streptococcus mutans and Streptococcus sanguinis in the Dental Biofilm

    doi: 10.1128/JB.187.21.7193-7203.2005

    Figure Lengend Snippet: Inhibition of oral streptococcal species by S. mutans UA140. 1, S. gordonii ; 2, S. pyogenes ; 3, S. oralis ; 4, S. mitis ATCC 33399; 5, S. mitis ATCC 903; 6, S. pneumoniae ; 7, S. cristatus ; 8, S. parasanguinis ; 9, S. sanguinis ATCC 10556; 10, S. sanguinis

    Article Snippet: To get a global view of how prevalent interspecies competition is between S. mutans and other oral streptococci, we analyzed the inhibitory spectrum of S. mutans strain UA140 against 11 streptococcal species, including members of the mitis, mutans, viridans, and pyogenic groups: S. gordonii ATCC 10558, S. oralis ATCC 10557, S. mitis ATCC 33399, S. mitis ATCC 903, S. pneumoniae , S. parasanguinis ATCC 15911, S. sanguinis ATCC 10556, S. sanguinis NY101, S. sobrinus OMZ176.

    Techniques: Inhibition

    (a) Light phase-contrast microscopy images of co-cultured human gingival fibroblasts (HGF) cells in different experimental conditions: HGF, HGF-HEMA, HGF/ Sm DS12, HGF-HEMA/ Sm DS12, HGF/ Sm ATCC and HGF-HEMA/ Sm ATCC after 48 h (left panel) and 72 h (right panel) of treatment. Arrows indicate dead cells. Magnification 10×. (b) Graphic representation of cell death percentage assessed by trypan blue dye exclusion test, after 48 and 72 h of treatment. Data are the mean (±SD) of three different consistent experiments. *48 h HGF % dead cells versus 48 h HGF-HEMA % dead cells P

    Journal: International Endodontic Journal

    Article Title: Anti-adhesive and pro-apoptotic effects of 2-hydroxyethyl methacrylate on human gingival fibroblasts co-cultured with Streptococcus mitis strains

    doi: 10.1111/j.1365-2591.2011.01935.x

    Figure Lengend Snippet: (a) Light phase-contrast microscopy images of co-cultured human gingival fibroblasts (HGF) cells in different experimental conditions: HGF, HGF-HEMA, HGF/ Sm DS12, HGF-HEMA/ Sm DS12, HGF/ Sm ATCC and HGF-HEMA/ Sm ATCC after 48 h (left panel) and 72 h (right panel) of treatment. Arrows indicate dead cells. Magnification 10×. (b) Graphic representation of cell death percentage assessed by trypan blue dye exclusion test, after 48 and 72 h of treatment. Data are the mean (±SD) of three different consistent experiments. *48 h HGF % dead cells versus 48 h HGF-HEMA % dead cells P

    Article Snippet: In the current study, the effects of a low concentration of HEMA at 48 and 72 h of exposure were analysed in primary HGF co-cultured with S. mitis clinical DS12 and ATCC 6249 bacterial strains to investigate prokaryotic and eukaryotic cell behaviour, in terms of viability, adhesion and apoptotic events.

    Techniques: Microscopy, Cell Culture

    (a) Immunofluorescence analysis of pro-collagen I expression of co-cultured human gingival fibroblasts (HGF) cells in different experimental conditions: HGF, HGF-HEMA, HGF/ Sm DS12, HGF-HEMA/ Sm DS12 and negative control (c−) after 48 h (left panel) and 72 h (right panel) of treatment. Magnification 40×. Green fluorescence of fluorescein isothiocyanate (FITC) refers to pro-collagen I labelling; blue fluorescence of DAPI (4-6 diamino-2-phenyl-indol)-counterstains nuclei. (b) Graphic representation of densitometric analysis of pro-collagen-I-positive area (±SD) determined by direct visual counting of ten fields (mean values) for each of five slides per sample at 40× magnification. HGF shows a physiological pro-collagen I expression, labelling vanishes in all other experimental points. *48 h HGF % fluorescent area versus 48 h HGF-HEMA fluorescent area P

    Journal: International Endodontic Journal

    Article Title: Anti-adhesive and pro-apoptotic effects of 2-hydroxyethyl methacrylate on human gingival fibroblasts co-cultured with Streptococcus mitis strains

    doi: 10.1111/j.1365-2591.2011.01935.x

    Figure Lengend Snippet: (a) Immunofluorescence analysis of pro-collagen I expression of co-cultured human gingival fibroblasts (HGF) cells in different experimental conditions: HGF, HGF-HEMA, HGF/ Sm DS12, HGF-HEMA/ Sm DS12 and negative control (c−) after 48 h (left panel) and 72 h (right panel) of treatment. Magnification 40×. Green fluorescence of fluorescein isothiocyanate (FITC) refers to pro-collagen I labelling; blue fluorescence of DAPI (4-6 diamino-2-phenyl-indol)-counterstains nuclei. (b) Graphic representation of densitometric analysis of pro-collagen-I-positive area (±SD) determined by direct visual counting of ten fields (mean values) for each of five slides per sample at 40× magnification. HGF shows a physiological pro-collagen I expression, labelling vanishes in all other experimental points. *48 h HGF % fluorescent area versus 48 h HGF-HEMA fluorescent area P

    Article Snippet: In the current study, the effects of a low concentration of HEMA at 48 and 72 h of exposure were analysed in primary HGF co-cultured with S. mitis clinical DS12 and ATCC 6249 bacterial strains to investigate prokaryotic and eukaryotic cell behaviour, in terms of viability, adhesion and apoptotic events.

    Techniques: Immunofluorescence, Expressing, Cell Culture, Negative Control, Fluorescence

    Bacterial viability, assessed by Live/Dead kit, of Streptococcus mitis strains co-cultured with human gingival fibroblasts (HGF) cells in different experimental conditions: HGF/ Sm DS12, HGF-HEMA/ Sm DS12, HGF/ Sm ATCC and HGF-HEMA/ Sm ATCC after 48 h (upper panel) and 72 h (lower panel) of treatment. Green fluorescence indicates viable bacteria (white arrow), and red fluorescence indicates dead bacteria (big red spots indicate HGF nuclei). Magnification 100×. Note that bacterial viability is high in all experimental conditions and does not seem to be influenced by HEMA and cells’ presence. Pictures are the most representative of three different consistent experiments.

    Journal: International Endodontic Journal

    Article Title: Anti-adhesive and pro-apoptotic effects of 2-hydroxyethyl methacrylate on human gingival fibroblasts co-cultured with Streptococcus mitis strains

    doi: 10.1111/j.1365-2591.2011.01935.x

    Figure Lengend Snippet: Bacterial viability, assessed by Live/Dead kit, of Streptococcus mitis strains co-cultured with human gingival fibroblasts (HGF) cells in different experimental conditions: HGF/ Sm DS12, HGF-HEMA/ Sm DS12, HGF/ Sm ATCC and HGF-HEMA/ Sm ATCC after 48 h (upper panel) and 72 h (lower panel) of treatment. Green fluorescence indicates viable bacteria (white arrow), and red fluorescence indicates dead bacteria (big red spots indicate HGF nuclei). Magnification 100×. Note that bacterial viability is high in all experimental conditions and does not seem to be influenced by HEMA and cells’ presence. Pictures are the most representative of three different consistent experiments.

    Article Snippet: In the current study, the effects of a low concentration of HEMA at 48 and 72 h of exposure were analysed in primary HGF co-cultured with S. mitis clinical DS12 and ATCC 6249 bacterial strains to investigate prokaryotic and eukaryotic cell behaviour, in terms of viability, adhesion and apoptotic events.

    Techniques: Cell Culture, Fluorescence

    (a) TUNEL detection of co-cultured human gingival fibroblasts (HGF) apoptotic nuclei in different experimental conditions: HGF-HEMA, HGF/ Sm DS12, HGF-HEMA/ Sm DS12 and negative control (c−) after 48 h (left panel) and 72 h (right panel) of treatment. Blue staining and arrowheads indicate negative nuclei, and brown staining and arrows indicate positive nuclei. Magnification 20×. (b) Graphical representation of TUNEL analysis. Five slides have been examined per sample. Apoptotic cells have been counted out of a total cell number and expressed as percentage. Physiological levels of apoptotic cells can be detected in HGF and HGF/ Sm DS12, and HGF-HEMA shows an increase in apoptotic nuclei percentage, more evident in HGF-HEMA/ Sm DS12. Values represented in the graph are means (±SD) n = 3 for all groups. *48 h HGF % apoptotic nuclei versus 48 HGF-HEMA % apoptotic nuclei P

    Journal: International Endodontic Journal

    Article Title: Anti-adhesive and pro-apoptotic effects of 2-hydroxyethyl methacrylate on human gingival fibroblasts co-cultured with Streptococcus mitis strains

    doi: 10.1111/j.1365-2591.2011.01935.x

    Figure Lengend Snippet: (a) TUNEL detection of co-cultured human gingival fibroblasts (HGF) apoptotic nuclei in different experimental conditions: HGF-HEMA, HGF/ Sm DS12, HGF-HEMA/ Sm DS12 and negative control (c−) after 48 h (left panel) and 72 h (right panel) of treatment. Blue staining and arrowheads indicate negative nuclei, and brown staining and arrows indicate positive nuclei. Magnification 20×. (b) Graphical representation of TUNEL analysis. Five slides have been examined per sample. Apoptotic cells have been counted out of a total cell number and expressed as percentage. Physiological levels of apoptotic cells can be detected in HGF and HGF/ Sm DS12, and HGF-HEMA shows an increase in apoptotic nuclei percentage, more evident in HGF-HEMA/ Sm DS12. Values represented in the graph are means (±SD) n = 3 for all groups. *48 h HGF % apoptotic nuclei versus 48 HGF-HEMA % apoptotic nuclei P

    Article Snippet: In the current study, the effects of a low concentration of HEMA at 48 and 72 h of exposure were analysed in primary HGF co-cultured with S. mitis clinical DS12 and ATCC 6249 bacterial strains to investigate prokaryotic and eukaryotic cell behaviour, in terms of viability, adhesion and apoptotic events.

    Techniques: TUNEL Assay, Cell Culture, Negative Control, Staining