s. aureus strains Search Results


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    ATCC s aureus strains s aureus 8325 4
    S Aureus Strains S Aureus 8325 4, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore staphylococcus aureus
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    JMI Laboratories s aureus strains
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    ATCC staphylococcus aureus strains different s aureus strains
    The proteolytic cleavage sites induced after treatment of bCAT, hCAT and CTL with different  S. aureus  strains (ATCC25923, ATCC49775, S1 and S2). The experimental molecular masses obtained after MALDI-TOF analysis, are indicated.
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    Becton Dickinson s aureus strains
    The proteolytic cleavage sites induced after treatment of bCAT, hCAT and CTL with different  S. aureus  strains (ATCC25923, ATCC49775, S1 and S2). The experimental molecular masses obtained after MALDI-TOF analysis, are indicated.
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    Clinical and Laboratory Standards Institute s aureus strains
    The proteolytic cleavage sites induced after treatment of bCAT, hCAT and CTL with different  S. aureus  strains (ATCC25923, ATCC49775, S1 and S2). The experimental molecular masses obtained after MALDI-TOF analysis, are indicated.
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    Bruker Corporation s aureus strains
    The proteolytic cleavage sites induced after treatment of bCAT, hCAT and CTL with different  S. aureus  strains (ATCC25923, ATCC49775, S1 and S2). The experimental molecular masses obtained after MALDI-TOF analysis, are indicated.
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    Toxin Technology Inc s aureus strains
    The proteolytic cleavage sites induced after treatment of bCAT, hCAT and CTL with different  S. aureus  strains (ATCC25923, ATCC49775, S1 and S2). The experimental molecular masses obtained after MALDI-TOF analysis, are indicated.
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    Thermo Fisher s aureus strains
    The proteolytic cleavage sites induced after treatment of bCAT, hCAT and CTL with different  S. aureus  strains (ATCC25923, ATCC49775, S1 and S2). The experimental molecular masses obtained after MALDI-TOF analysis, are indicated.
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    Quotient Bioresearch s aureus strains
    The proteolytic cleavage sites induced after treatment of bCAT, hCAT and CTL with different  S. aureus  strains (ATCC25923, ATCC49775, S1 and S2). The experimental molecular masses obtained after MALDI-TOF analysis, are indicated.
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    Eppendorf AG s aureus strains
    The proteolytic cleavage sites induced after treatment of bCAT, hCAT and CTL with different  S. aureus  strains (ATCC25923, ATCC49775, S1 and S2). The experimental molecular masses obtained after MALDI-TOF analysis, are indicated.
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    ATCC s aureus strain
    Laurdan emission spectra in three S. aureus strains – S. aureus <t>ATCC</t> 25923 and two MRSA isolates, S. aureus Sa1 and S. aureus Sa3 – at 24, 48, 72 and 144 h after bacterial growth at 37 °C. The excitation wavelength was 350 nm. Data presented are from a single experiment. Intensities at 440 and 490 nm were used to calculate excitation generalized polarization values ( GP exc ).
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    Ridom GmbH s aureus strains
    Laurdan emission spectra in three S. aureus strains – S. aureus <t>ATCC</t> 25923 and two MRSA isolates, S. aureus Sa1 and S. aureus Sa3 – at 24, 48, 72 and 144 h after bacterial growth at 37 °C. The excitation wavelength was 350 nm. Data presented are from a single experiment. Intensities at 440 and 490 nm were used to calculate excitation generalized polarization values ( GP exc ).
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    ATCC s aureus strains sa113
    Detailed analyses of protein accumulation of selected proteins in S. aureus <t>SA113</t> and its isogenic nreABC mutant depending on nitrate availability under anaerobic conditions. 2D-PAGE was performed with cytoplasmic protein extracts of S. aureus SA113 wild
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    ATCC s aureus strain atcc 33753
    Detailed analyses of protein accumulation of selected proteins in S. aureus <t>SA113</t> and its isogenic nreABC mutant depending on nitrate availability under anaerobic conditions. 2D-PAGE was performed with cytoplasmic protein extracts of S. aureus SA113 wild
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    ATCC s aureus strains m
    Detailed analyses of protein accumulation of selected proteins in S. aureus <t>SA113</t> and its isogenic nreABC mutant depending on nitrate availability under anaerobic conditions. 2D-PAGE was performed with cytoplasmic protein extracts of S. aureus SA113 wild
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    ATCC s aureus strain newman d2c
    Inhibitory effects of erianin (different levels) against srtA from Staphylococcus aureus <t>Newman</t> <t>D2C</t> in vitro.
    S Aureus Strain Newman D2c, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC s aureus strains 6850
    Inhibitory effects of erianin (different levels) against srtA from Staphylococcus aureus <t>Newman</t> <t>D2C</t> in vitro.
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    ATCC toxin producing s aureus strains
    Inhibitory effects of erianin (different levels) against srtA from Staphylococcus aureus <t>Newman</t> <t>D2C</t> in vitro.
    Toxin Producing S Aureus Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC s aureus strains newbould
    Inhibitory effects of erianin (different levels) against srtA from Staphylococcus aureus <t>Newman</t> <t>D2C</t> in vitro.
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    ATCC multidrug resistant s aureus strain
    <t>CM05</t> cfr plasmid insertion region and recombination/excision scheme. (A) The 15.5-kb cfr region is inserted between bases 1256 and 1257 in 23S rRNA allele 4 ( rrn4 rRNA operon) in CM05. The insertion in allele 4 (flanked by MW1986 and ilvA genes) is annotated
    Multidrug Resistant S Aureus Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher s aureus strains primers
    <t>CM05</t> cfr plasmid insertion region and recombination/excision scheme. (A) The 15.5-kb cfr region is inserted between bases 1256 and 1257 in 23S rRNA allele 4 ( rrn4 rRNA operon) in CM05. The insertion in allele 4 (flanked by MW1986 and ilvA genes) is annotated
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    ATCC s aureus strains atcc 19685
    <t>CM05</t> cfr plasmid insertion region and recombination/excision scheme. (A) The 15.5-kb cfr region is inserted between bases 1256 and 1257 in 23S rRNA allele 4 ( rrn4 rRNA operon) in CM05. The insertion in allele 4 (flanked by MW1986 and ilvA genes) is annotated
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    ATCC s aureus strains atcc 27664
    <t>CM05</t> cfr plasmid insertion region and recombination/excision scheme. (A) The 15.5-kb cfr region is inserted between bases 1256 and 1257 in 23S rRNA allele 4 ( rrn4 rRNA operon) in CM05. The insertion in allele 4 (flanked by MW1986 and ilvA genes) is annotated
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    ATCC s aureus strains 33592
    <t>CM05</t> cfr plasmid insertion region and recombination/excision scheme. (A) The 15.5-kb cfr region is inserted between bases 1256 and 1257 in 23S rRNA allele 4 ( rrn4 rRNA operon) in CM05. The insertion in allele 4 (flanked by MW1986 and ilvA genes) is annotated
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    ATCC s aureus strains mbt 5040
    Lysostaphin did not disrupt biofilms formed by lysostaphin-resistant S. aureus variants. Tissue culture wells (three for each sample) of a microtiter plate were inoculated with ∼10 8 CFU of S. aureus strain SA113, SA5 LysoR, MBT 5040, or <t>MBT</t> 5040 LysoR (as indicated). After we allowed 48 h for biofilm formation, the wells were washed twice and then incubated with (+) or without (−) 50 μg of lysostaphin/ml in PBS for 3 h. Following incubation, the wells were washed again and stained with safranin to visualize biofilms.
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    ATCC s aureus strains atcc 12692
    Lysostaphin did not disrupt biofilms formed by lysostaphin-resistant S. aureus variants. Tissue culture wells (three for each sample) of a microtiter plate were inoculated with ∼10 8 CFU of S. aureus strain SA113, SA5 LysoR, MBT 5040, or <t>MBT</t> 5040 LysoR (as indicated). After we allowed 48 h for biofilm formation, the wells were washed twice and then incubated with (+) or without (−) 50 μg of lysostaphin/ml in PBS for 3 h. Following incubation, the wells were washed again and stained with safranin to visualize biofilms.
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    ATCC s aureus strain atcc 700699
    Binding and activity of CF-301. (A) Effects of serum and medium pretreatments on CF-301 binding to S. aureus MW2 determined by differential interference contrast (DIC) and fluorescence microscopy. Exposures of 3 ms (human, rabbit, and mouse plus HSA samples) and 3 s (rat, mouse, and caMHB samples) were used to visualize CF-301 RHOD . MIC values for each condition are indicated (micrograms per milliliter). (B) Effect of HSA on CF-301 binding to S. aureus <t>ATCC</t> 700699 determined by deconvolution microscopy. Maximum-intensity projections are shown. CF-301 RHOD , red; DAPI, blue (scale bar, 2 μm). (C) Effect of CF-301 on HuLYZ binding to S. aureus ATCC 700699 determined by deconvolution microscopy. Maximum-intensity projections are shown. HuLYZ AF , green; DAPI, blue (scale bar, 4 μm). (D) Bactericidal activity of CF-301 in human serum and caMHB determined by TEM. CF-301 was tested at 10×, 1×, and 0.1× MIC levels for each environment. Direct magnification values: control, ×13,000; 10× MIC, ×2,600; 1× and 0.1× MIC, ×6,600. Scale bars, 1 μm.
    S Aureus Strain Atcc 700699, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC growth conditions s aureus strains mw2
    Binding and activity of CF-301. (A) Effects of serum and medium pretreatments on CF-301 binding to S. aureus MW2 determined by differential interference contrast (DIC) and fluorescence microscopy. Exposures of 3 ms (human, rabbit, and mouse plus HSA samples) and 3 s (rat, mouse, and caMHB samples) were used to visualize CF-301 RHOD . MIC values for each condition are indicated (micrograms per milliliter). (B) Effect of HSA on CF-301 binding to S. aureus <t>ATCC</t> 700699 determined by deconvolution microscopy. Maximum-intensity projections are shown. CF-301 RHOD , red; DAPI, blue (scale bar, 2 μm). (C) Effect of CF-301 on HuLYZ binding to S. aureus ATCC 700699 determined by deconvolution microscopy. Maximum-intensity projections are shown. HuLYZ AF , green; DAPI, blue (scale bar, 4 μm). (D) Bactericidal activity of CF-301 in human serum and caMHB determined by TEM. CF-301 was tested at 10×, 1×, and 0.1× MIC levels for each environment. Direct magnification values: control, ×13,000; 10× MIC, ×2,600; 1× and 0.1× MIC, ×6,600. Scale bars, 1 μm.
    Growth Conditions S Aureus Strains Mw2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC s aureus strains atcc 13709 mssa
    Binding and activity of CF-301. (A) Effects of serum and medium pretreatments on CF-301 binding to S. aureus MW2 determined by differential interference contrast (DIC) and fluorescence microscopy. Exposures of 3 ms (human, rabbit, and mouse plus HSA samples) and 3 s (rat, mouse, and caMHB samples) were used to visualize CF-301 RHOD . MIC values for each condition are indicated (micrograms per milliliter). (B) Effect of HSA on CF-301 binding to S. aureus <t>ATCC</t> 700699 determined by deconvolution microscopy. Maximum-intensity projections are shown. CF-301 RHOD , red; DAPI, blue (scale bar, 2 μm). (C) Effect of CF-301 on HuLYZ binding to S. aureus ATCC 700699 determined by deconvolution microscopy. Maximum-intensity projections are shown. HuLYZ AF , green; DAPI, blue (scale bar, 4 μm). (D) Bactericidal activity of CF-301 in human serum and caMHB determined by TEM. CF-301 was tested at 10×, 1×, and 0.1× MIC levels for each environment. Direct magnification values: control, ×13,000; 10× MIC, ×2,600; 1× and 0.1× MIC, ×6,600. Scale bars, 1 μm.
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    ATCC s aureus strains atcc 19095
    Binding and activity of CF-301. (A) Effects of serum and medium pretreatments on CF-301 binding to S. aureus MW2 determined by differential interference contrast (DIC) and fluorescence microscopy. Exposures of 3 ms (human, rabbit, and mouse plus HSA samples) and 3 s (rat, mouse, and caMHB samples) were used to visualize CF-301 RHOD . MIC values for each condition are indicated (micrograms per milliliter). (B) Effect of HSA on CF-301 binding to S. aureus <t>ATCC</t> 700699 determined by deconvolution microscopy. Maximum-intensity projections are shown. CF-301 RHOD , red; DAPI, blue (scale bar, 2 μm). (C) Effect of CF-301 on HuLYZ binding to S. aureus ATCC 700699 determined by deconvolution microscopy. Maximum-intensity projections are shown. HuLYZ AF , green; DAPI, blue (scale bar, 4 μm). (D) Bactericidal activity of CF-301 in human serum and caMHB determined by TEM. CF-301 was tested at 10×, 1×, and 0.1× MIC levels for each environment. Direct magnification values: control, ×13,000; 10× MIC, ×2,600; 1× and 0.1× MIC, ×6,600. Scale bars, 1 μm.
    S Aureus Strains Atcc 19095, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    The proteolytic cleavage sites induced after treatment of bCAT, hCAT and CTL with different  S. aureus  strains (ATCC25923, ATCC49775, S1 and S2). The experimental molecular masses obtained after MALDI-TOF analysis, are indicated.

    Journal: PLoS ONE

    Article Title: Cateslytin, a Chromogranin A Derived Peptide Is Active against Staphylococcus aureus and Resistant to Degradation by Its Proteases

    doi: 10.1371/journal.pone.0068993

    Figure Lengend Snippet: The proteolytic cleavage sites induced after treatment of bCAT, hCAT and CTL with different S. aureus strains (ATCC25923, ATCC49775, S1 and S2). The experimental molecular masses obtained after MALDI-TOF analysis, are indicated.

    Article Snippet: Isolation and characterization of Staphylococcus aureus strains Different S. aureus strains were used to demonstrate the peptide antimicrobial activity and subsequently, the peptide degradation: strains ATCC 25923, ATCC49775, S1 and S2, were provided by the Institute of Bacteriology, Strasbourg, France.

    Techniques: CTL Assay

    Laurdan emission spectra in three S. aureus strains – S. aureus ATCC 25923 and two MRSA isolates, S. aureus Sa1 and S. aureus Sa3 – at 24, 48, 72 and 144 h after bacterial growth at 37 °C. The excitation wavelength was 350 nm. Data presented are from a single experiment. Intensities at 440 and 490 nm were used to calculate excitation generalized polarization values ( GP exc ).

    Journal: Data in Brief

    Article Title: Data on Laurdan spectroscopic analyses to compare membrane fluidity between susceptible and multidrug-resistant bacteria

    doi: 10.1016/j.dib.2018.09.106

    Figure Lengend Snippet: Laurdan emission spectra in three S. aureus strains – S. aureus ATCC 25923 and two MRSA isolates, S. aureus Sa1 and S. aureus Sa3 – at 24, 48, 72 and 144 h after bacterial growth at 37 °C. The excitation wavelength was 350 nm. Data presented are from a single experiment. Intensities at 440 and 490 nm were used to calculate excitation generalized polarization values ( GP exc ).

    Article Snippet: Data refers to three E. coli strains – E. coli ATCC 25922 and two multidrug-resistant clinical isolates, E. coli EC2 and E. coli EC3 ( ) – and to three S. aureus strains – S. aureus ATCC 25923 and two methicillin-resistant S. aureus (MRSA) isolates, S. aureus Sa1 and S. aureus Sa3 ( ).

    Techniques:

    S. aureus colony forming units (CFU) found in skin (checked bars), spleen (solid bars), and kidney (open bars) at various times (in hrs on the X -axis) after epicutaneous inoculation. A. S. aureus ATCC strain #25923 in C57BL/6 mice; B. S. aureus ATCC strain #25923 in cyclophosphamide-treated C57BL/6 mice; C. S. aureus strain Newman in C57BL/6 mice; D. S. aureus strain ATCC #25923 in Balb/c mice. Note in panel A that no CFU were found in kidney and spleen at 1 h after inoculation, indicating lack of contamination during the organ harvesting procedure; also, the difference in skin CFU between 1 and 48 h was significant ( P

    Journal: Microbial pathogenesis

    Article Title: Systemic dissemination and cutaneous damage in a mouse model of staphylococcal skin infections

    doi: 10.1016/j.micpath.2009.04.007

    Figure Lengend Snippet: S. aureus colony forming units (CFU) found in skin (checked bars), spleen (solid bars), and kidney (open bars) at various times (in hrs on the X -axis) after epicutaneous inoculation. A. S. aureus ATCC strain #25923 in C57BL/6 mice; B. S. aureus ATCC strain #25923 in cyclophosphamide-treated C57BL/6 mice; C. S. aureus strain Newman in C57BL/6 mice; D. S. aureus strain ATCC #25923 in Balb/c mice. Note in panel A that no CFU were found in kidney and spleen at 1 h after inoculation, indicating lack of contamination during the organ harvesting procedure; also, the difference in skin CFU between 1 and 48 h was significant ( P

    Article Snippet: S. aureus strain ATCC #25923 has been shown to be virulent in other animal models of staphylococcal infection [ , ].

    Techniques: Mouse Assay

    Location of bacteria in different skin layers, expressed as percent of microscopic fields with organisms at each site on the Y -axis, for locations in the stratum corneum or crusts (checked bars), epidermal keratinocytes (solid bars), or dermis (open bars); times after epicutaneous inoculation are given in hrs on the X -axis. A. S. aureus ATCC strain #25923 in C57BL/6 mice; B. S. aureus ATCC strain #25923 in cyclophosphamide-treated C57BL/6 mice; C. S. aureus strain Newman in C57BL/6 mice; D. S. aureus strain ATCC #25923 in Balb/c mice. Data are from 4–11 animals per point, studied in 3–5 experiments.

    Journal: Microbial pathogenesis

    Article Title: Systemic dissemination and cutaneous damage in a mouse model of staphylococcal skin infections

    doi: 10.1016/j.micpath.2009.04.007

    Figure Lengend Snippet: Location of bacteria in different skin layers, expressed as percent of microscopic fields with organisms at each site on the Y -axis, for locations in the stratum corneum or crusts (checked bars), epidermal keratinocytes (solid bars), or dermis (open bars); times after epicutaneous inoculation are given in hrs on the X -axis. A. S. aureus ATCC strain #25923 in C57BL/6 mice; B. S. aureus ATCC strain #25923 in cyclophosphamide-treated C57BL/6 mice; C. S. aureus strain Newman in C57BL/6 mice; D. S. aureus strain ATCC #25923 in Balb/c mice. Data are from 4–11 animals per point, studied in 3–5 experiments.

    Article Snippet: S. aureus strain ATCC #25923 has been shown to be virulent in other animal models of staphylococcal infection [ , ].

    Techniques: Mouse Assay

    Location of bacteria after epicutaneous inoculation of C57BL/6 mice with S. aureus ATCC strain #25923 24 h previously (photomicrographs of tissue gram-stained sections at 1000× original magnification under oil immersion): A. Section of normal uninoculated C57BL/6 mouse skin with the bracket marking the epidermal keratinocyte layers; the stratum corneum lies above these layers and the dermis lies below; B. Bacteria located in epidermal keratinocytes (arrow); C. Bacteria located in the dermis (arrow); D. Bacteria associated with a dermal blood vessel in a cyclophosphamide-treated mouse.

    Journal: Microbial pathogenesis

    Article Title: Systemic dissemination and cutaneous damage in a mouse model of staphylococcal skin infections

    doi: 10.1016/j.micpath.2009.04.007

    Figure Lengend Snippet: Location of bacteria after epicutaneous inoculation of C57BL/6 mice with S. aureus ATCC strain #25923 24 h previously (photomicrographs of tissue gram-stained sections at 1000× original magnification under oil immersion): A. Section of normal uninoculated C57BL/6 mouse skin with the bracket marking the epidermal keratinocyte layers; the stratum corneum lies above these layers and the dermis lies below; B. Bacteria located in epidermal keratinocytes (arrow); C. Bacteria located in the dermis (arrow); D. Bacteria associated with a dermal blood vessel in a cyclophosphamide-treated mouse.

    Article Snippet: S. aureus strain ATCC #25923 has been shown to be virulent in other animal models of staphylococcal infection [ , ].

    Techniques: Mouse Assay, Staining

    Infected hair follicle outlets (checked bars), infected hair follicle outlets with inflammatory cell infiltration (solid bars), and deep (greater than 100 µm from the skin surface) hair follicle infections (open bars) at 6 or 24 h after inoculation with S. aureus ATCC strain #25923 in C57BL/6 mice.

    Journal: Microbial pathogenesis

    Article Title: Systemic dissemination and cutaneous damage in a mouse model of staphylococcal skin infections

    doi: 10.1016/j.micpath.2009.04.007

    Figure Lengend Snippet: Infected hair follicle outlets (checked bars), infected hair follicle outlets with inflammatory cell infiltration (solid bars), and deep (greater than 100 µm from the skin surface) hair follicle infections (open bars) at 6 or 24 h after inoculation with S. aureus ATCC strain #25923 in C57BL/6 mice.

    Article Snippet: S. aureus strain ATCC #25923 has been shown to be virulent in other animal models of staphylococcal infection [ , ].

    Techniques: Infection, Mouse Assay

    Growth curve for S. aureus strain ATCC 25923 in the presence or absence of CT. ■, S. aureus ATCC 25923 plus 1 μ g · mL CT; Δ, S. aureus ATCC 25923 plus 2 μ g · mL CT; ∗, S. aureus ATCC 25923 plus 4 μ g · mL CT; ▲, S. aureus ATCC 25923 plus 8 μ g · mL CT; □, S. aureus ATCC 25923 plus 16 μ g · mL CT; ♦, untreated S. aureus ATCC 25923.

    Journal: Journal of Biomedicine and Biotechnology

    Article Title: Genome-Wide Transcriptional Profiling of the Response of Staphylococcus aureus to Cryptotanshinone

    doi: 10.1155/2009/617509

    Figure Lengend Snippet: Growth curve for S. aureus strain ATCC 25923 in the presence or absence of CT. ■, S. aureus ATCC 25923 plus 1 μ g · mL CT; Δ, S. aureus ATCC 25923 plus 2 μ g · mL CT; ∗, S. aureus ATCC 25923 plus 4 μ g · mL CT; ▲, S. aureus ATCC 25923 plus 8 μ g · mL CT; □, S. aureus ATCC 25923 plus 16 μ g · mL CT; ♦, untreated S. aureus ATCC 25923.

    Article Snippet: Synergistic Study A standard checkerboard assay was performed to assess the interaction of combination (CT and TMP/SXT) against S. aureus strain ATCC 25923 by a well-established method [ ].

    Techniques:

    Antibiofilm activities of alizarin against  S. aureus  and  S. epidermidis . The antibiofilm activities (OD 570 ) of alizarin were determined against two methicillin-sensitive  S. aureus  strains (MSSA, ATCC 25923 and ATCC 6538), a methicillin-resistant  S. aureus  strain (MRSA, MW2) ( a–c ), and  S. epidermidis  (ATCC 14990) ( d ). Two independent experiments were conducted (12 wells per sample); error bars indicate standard deviations. * P

    Journal: Scientific Reports

    Article Title: Calcium-chelating alizarin and other anthraquinones inhibit biofilm formation and the hemolytic activity of Staphylococcus aureus

    doi: 10.1038/srep19267

    Figure Lengend Snippet: Antibiofilm activities of alizarin against S. aureus and S. epidermidis . The antibiofilm activities (OD 570 ) of alizarin were determined against two methicillin-sensitive S. aureus strains (MSSA, ATCC 25923 and ATCC 6538), a methicillin-resistant S. aureus strain (MRSA, MW2) ( a–c ), and S. epidermidis (ATCC 14990) ( d ). Two independent experiments were conducted (12 wells per sample); error bars indicate standard deviations. * P

    Article Snippet: Bacterial strains, growth measurements, and materials The following bacterial strains were used in the present study; methicillin-sensitive S. aureus strains (MSSA; ATCC 25923 and ATCC 6538), a methicillin-resistant S. aureus strain (ATCC BAA-1707, MW2), S. epidermidis (ATCC 14990), Pseudomonas aeruginosa PAO1 (ATCC 15692), and Escherichia coli O157:H7 (ATCC 43895, EDL933).

    Techniques:

    Detailed analyses of protein accumulation of selected proteins in S. aureus SA113 and its isogenic nreABC mutant depending on nitrate availability under anaerobic conditions. 2D-PAGE was performed with cytoplasmic protein extracts of S. aureus SA113 wild

    Journal:

    Article Title: Characterization of the Oxygen-Responsive NreABC Regulon of Staphylococcus aureus ▿ ▿ †

    doi: 10.1128/JB.00905-08

    Figure Lengend Snippet: Detailed analyses of protein accumulation of selected proteins in S. aureus SA113 and its isogenic nreABC mutant depending on nitrate availability under anaerobic conditions. 2D-PAGE was performed with cytoplasmic protein extracts of S. aureus SA113 wild

    Article Snippet: S. aureus strains SA113 (ATCC 35556), a derivative of S. aureus NCTC8325 , and COL ( ) were used in this study.

    Techniques: Mutagenesis, Polyacrylamide Gel Electrophoresis

    The influence of nreABC deletion on the cytoplasmic protein pattern of S. aureus SA113. 2D-PAGE was performed with cytoplasmic protein extracts of S. aureus SA113 wild type and its isogenic nreABC mutant cultivated anaerobically in a fermentor (to an

    Journal:

    Article Title: Characterization of the Oxygen-Responsive NreABC Regulon of Staphylococcus aureus ▿ ▿ †

    doi: 10.1128/JB.00905-08

    Figure Lengend Snippet: The influence of nreABC deletion on the cytoplasmic protein pattern of S. aureus SA113. 2D-PAGE was performed with cytoplasmic protein extracts of S. aureus SA113 wild type and its isogenic nreABC mutant cultivated anaerobically in a fermentor (to an

    Article Snippet: S. aureus strains SA113 (ATCC 35556), a derivative of S. aureus NCTC8325 , and COL ( ) were used in this study.

    Techniques: Polyacrylamide Gel Electrophoresis, Mutagenesis

    Venn diagrams of genes differentially transcribed in the S. aureus SA113 nreABC mutant. In each circle, the total number of genes downregulated (A) or upregulated (B) in the nreABC mutant (Δ nreABC ), compared to the wild type, under a given condition

    Journal:

    Article Title: Characterization of the Oxygen-Responsive NreABC Regulon of Staphylococcus aureus ▿ ▿ †

    doi: 10.1128/JB.00905-08

    Figure Lengend Snippet: Venn diagrams of genes differentially transcribed in the S. aureus SA113 nreABC mutant. In each circle, the total number of genes downregulated (A) or upregulated (B) in the nreABC mutant (Δ nreABC ), compared to the wild type, under a given condition

    Article Snippet: S. aureus strains SA113 (ATCC 35556), a derivative of S. aureus NCTC8325 , and COL ( ) were used in this study.

    Techniques: Mutagenesis

    Biofilm production after ethanol ( a ) or Isopropyl Alcohol ( b ) exposure for 24 h. Mean ± SEM optical density (OD) at 570 nm of stained biofilms in 96 well plates after 24 h exposure to 40%, 60%, 80%, and 95% alcohols compared to normal saline 0.9% (NS) ( n = 8 each). SE 35984 S. epidermidis ATCC 35984, SE M7 S. epidermidis M7, MSSA 35556 methicillin-susceptible S. aureus ATCC 35556, MSSA 29213 methicillin-susceptible S. aureus ATCC 29213, MRSA 32 methicillin-resistant S. aureus clinical strain L32, MRSA 43300 methicillin-resistant S. aureus ATCC 43300, EtOH ethanol, SEM standard error of the mean. ( Asterisk ) Statistically significant compared to NS ( p

    Journal: Infectious Diseases and Therapy

    Article Title: Ethanol and Isopropyl Alcohol Exposure Increases Biofilm Formation in Staphylococcus aureus and Staphylococcus epidermidis

    doi: 10.1007/s40121-015-0065-y

    Figure Lengend Snippet: Biofilm production after ethanol ( a ) or Isopropyl Alcohol ( b ) exposure for 24 h. Mean ± SEM optical density (OD) at 570 nm of stained biofilms in 96 well plates after 24 h exposure to 40%, 60%, 80%, and 95% alcohols compared to normal saline 0.9% (NS) ( n = 8 each). SE 35984 S. epidermidis ATCC 35984, SE M7 S. epidermidis M7, MSSA 35556 methicillin-susceptible S. aureus ATCC 35556, MSSA 29213 methicillin-susceptible S. aureus ATCC 29213, MRSA 32 methicillin-resistant S. aureus clinical strain L32, MRSA 43300 methicillin-resistant S. aureus ATCC 43300, EtOH ethanol, SEM standard error of the mean. ( Asterisk ) Statistically significant compared to NS ( p

    Article Snippet: Bacterial Strains Five ATCC Staphylococcal strains were evaluated: a biofilm-producing S. epidermidis strain (ATCC 35984; RP62A [ATCC® , Manassas, Virginia]) and its isogenic, slime-negative, biofilm-deficient mutant derived from chemical mutagenesis (M7), two biofilm-forming methicillin-susceptible S. aureus strains (ATCC 35556 and ATCC 29213) and a biofilm-forming methicillin-resistant S. aureus strain (MRSA; ATCC 43300) [ – ].

    Techniques: Staining

    Inhibitory effects of erianin (different levels) against srtA from Staphylococcus aureus Newman D2C in vitro.

    Journal: Toxins

    Article Title: Erianin against Staphylococcus aureus Infection via Inhibiting Sortase A

    doi: 10.3390/toxins10100385

    Figure Lengend Snippet: Inhibitory effects of erianin (different levels) against srtA from Staphylococcus aureus Newman D2C in vitro.

    Article Snippet: S. aureus strain Newman D2C (ATCC25904) was commercially obtained from the American Type Culture Collection (ATCC) and used in this study.

    Techniques: In Vitro

    Growth curves of Staphylococcus aureus strain Newman D2C with or without erianin and gene knockout mutant ΔsrtA in brain-heart infusion (BHI). Symbol ●, ■, ▲, ▼, ◆, ○ and □ represent gene knockout mutant ΔsrtA, Staphylococcus aureus strain Newman D2C culture with 0, 4, 16, 32, 64 and 128 μg/mL erianin, respectively.

    Journal: Toxins

    Article Title: Erianin against Staphylococcus aureus Infection via Inhibiting Sortase A

    doi: 10.3390/toxins10100385

    Figure Lengend Snippet: Growth curves of Staphylococcus aureus strain Newman D2C with or without erianin and gene knockout mutant ΔsrtA in brain-heart infusion (BHI). Symbol ●, ■, ▲, ▼, ◆, ○ and □ represent gene knockout mutant ΔsrtA, Staphylococcus aureus strain Newman D2C culture with 0, 4, 16, 32, 64 and 128 μg/mL erianin, respectively.

    Article Snippet: S. aureus strain Newman D2C (ATCC25904) was commercially obtained from the American Type Culture Collection (ATCC) and used in this study.

    Techniques: Gene Knockout, Mutagenesis

    CM05 cfr plasmid insertion region and recombination/excision scheme. (A) The 15.5-kb cfr region is inserted between bases 1256 and 1257 in 23S rRNA allele 4 ( rrn4 rRNA operon) in CM05. The insertion in allele 4 (flanked by MW1986 and ilvA genes) is annotated

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Genetic Environment and Stability of cfr in Methicillin-Resistant Staphylococcus aureus CM05

    doi: 10.1128/AAC.05420-11

    Figure Lengend Snippet: CM05 cfr plasmid insertion region and recombination/excision scheme. (A) The 15.5-kb cfr region is inserted between bases 1256 and 1257 in 23S rRNA allele 4 ( rrn4 rRNA operon) in CM05. The insertion in allele 4 (flanked by MW1986 and ilvA genes) is annotated

    Article Snippet: S. aureus strains CM05, CM05Δ, and ATCC 29213 were cultured aerobically at 37°C on cation-adjusted Mueller-Hinton II agar (MHA; Becton Dickinson, Franklin Lakes, NJ) or in Mueller-Hinton II broth (MHB).

    Techniques: Plasmid Preparation

    LZD plating discrepancies reveal a subpopulation of cfr -negative CM05 (CM05Δ).

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Genetic Environment and Stability of cfr in Methicillin-Resistant Staphylococcus aureus CM05

    doi: 10.1128/AAC.05420-11

    Figure Lengend Snippet: LZD plating discrepancies reveal a subpopulation of cfr -negative CM05 (CM05Δ).

    Article Snippet: S. aureus strains CM05, CM05Δ, and ATCC 29213 were cultured aerobically at 37°C on cation-adjusted Mueller-Hinton II agar (MHA; Becton Dickinson, Franklin Lakes, NJ) or in Mueller-Hinton II broth (MHB).

    Techniques:

    Model of the insertion of the 15.5-kb cfr -containing sequence into the CM05 chromosome. (A) The β/ six ). Sites I and II of the recognition site are

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Genetic Environment and Stability of cfr in Methicillin-Resistant Staphylococcus aureus CM05

    doi: 10.1128/AAC.05420-11

    Figure Lengend Snippet: Model of the insertion of the 15.5-kb cfr -containing sequence into the CM05 chromosome. (A) The β/ six ). Sites I and II of the recognition site are

    Article Snippet: S. aureus strains CM05, CM05Δ, and ATCC 29213 were cultured aerobically at 37°C on cation-adjusted Mueller-Hinton II agar (MHA; Becton Dickinson, Franklin Lakes, NJ) or in Mueller-Hinton II broth (MHB).

    Techniques: Sequencing

    PCR analysis of rrn alleles in S. aureus CM05. All six rRNA rrn loci (5S, 16S, and 23S rRNA genes with flanking sequences) were amplified by PCR for CM05 and ATCC 29213 (positive-control 6- rrn allele reference strain). CM05 rrn4 was not amplified, due

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Genetic Environment and Stability of cfr in Methicillin-Resistant Staphylococcus aureus CM05

    doi: 10.1128/AAC.05420-11

    Figure Lengend Snippet: PCR analysis of rrn alleles in S. aureus CM05. All six rRNA rrn loci (5S, 16S, and 23S rRNA genes with flanking sequences) were amplified by PCR for CM05 and ATCC 29213 (positive-control 6- rrn allele reference strain). CM05 rrn4 was not amplified, due

    Article Snippet: S. aureus strains CM05, CM05Δ, and ATCC 29213 were cultured aerobically at 37°C on cation-adjusted Mueller-Hinton II agar (MHA; Becton Dickinson, Franklin Lakes, NJ) or in Mueller-Hinton II broth (MHB).

    Techniques: Polymerase Chain Reaction, Amplification, Positive Control

    Growth profiles of strains CM05 and CM05Δ. The growth rates of CM05 and CM05Δ were compared in MHB. The average OD 600 values of three independent cultures for each strain are shown for each time point, along with the standard deviations.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Genetic Environment and Stability of cfr in Methicillin-Resistant Staphylococcus aureus CM05

    doi: 10.1128/AAC.05420-11

    Figure Lengend Snippet: Growth profiles of strains CM05 and CM05Δ. The growth rates of CM05 and CM05Δ were compared in MHB. The average OD 600 values of three independent cultures for each strain are shown for each time point, along with the standard deviations.

    Article Snippet: S. aureus strains CM05, CM05Δ, and ATCC 29213 were cultured aerobically at 37°C on cation-adjusted Mueller-Hinton II agar (MHA; Becton Dickinson, Franklin Lakes, NJ) or in Mueller-Hinton II broth (MHB).

    Techniques:

    MIC profiles of a mixed CM05–CM05Δ culture passaged on gradient plates containing TR-700 or LZD. MIC testing of total-cell populations recovered from the edges of visible growth inhibition zones on antibiotic gradient plates showed no

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Genetic Environment and Stability of cfr in Methicillin-Resistant Staphylococcus aureus CM05

    doi: 10.1128/AAC.05420-11

    Figure Lengend Snippet: MIC profiles of a mixed CM05–CM05Δ culture passaged on gradient plates containing TR-700 or LZD. MIC testing of total-cell populations recovered from the edges of visible growth inhibition zones on antibiotic gradient plates showed no

    Article Snippet: S. aureus strains CM05, CM05Δ, and ATCC 29213 were cultured aerobically at 37°C on cation-adjusted Mueller-Hinton II agar (MHA; Becton Dickinson, Franklin Lakes, NJ) or in Mueller-Hinton II broth (MHB).

    Techniques: Inhibition

    LZD plating discrepancies reveal a subpopulation of cfr -negative CM05 (CM05Δ).

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Genetic Environment and Stability of cfr in Methicillin-Resistant Staphylococcus aureus CM05

    doi: 10.1128/AAC.05420-11

    Figure Lengend Snippet: LZD plating discrepancies reveal a subpopulation of cfr -negative CM05 (CM05Δ).

    Article Snippet: S. aureus strains CM05, CM05Δ, and ATCC 29213 were cultured aerobically at 37°C on cation-adjusted Mueller-Hinton II agar (MHA; Becton Dickinson, Franklin Lakes, NJ) or in Mueller-Hinton II broth (MHB).

    Techniques:

    Serial passage of CM05 under nonselective conditions. A clonal CM05 cfr -positive culture was serially passaged in MHB 75 times (1:1,000 dilutions). Every 5th passage, the ratio of cfr -positive to cfr -negative colonies was determined. At passage 30, LZD

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Genetic Environment and Stability of cfr in Methicillin-Resistant Staphylococcus aureus CM05

    doi: 10.1128/AAC.05420-11

    Figure Lengend Snippet: Serial passage of CM05 under nonselective conditions. A clonal CM05 cfr -positive culture was serially passaged in MHB 75 times (1:1,000 dilutions). Every 5th passage, the ratio of cfr -positive to cfr -negative colonies was determined. At passage 30, LZD

    Article Snippet: S. aureus strains CM05, CM05Δ, and ATCC 29213 were cultured aerobically at 37°C on cation-adjusted Mueller-Hinton II agar (MHA; Becton Dickinson, Franklin Lakes, NJ) or in Mueller-Hinton II broth (MHB).

    Techniques:

    PTS-dependent carbohydrate uptake contributes disproportionately to the nonrespiratory growth of S. aureus . (A) Glucose yield (milligrams of glucose consumed per milligram [dry weight] of biomass) of S. aureus COL under respiratory and nonrespiratory (anaerobic and NO-stressed) conditions ( n = 3; error bars show the standard error of the mean). S. aureus consumes ~3-fold more glucose per cell under nonrespiratory conditions. Statistical significance was calculated with a Student two-sided t test (*, P ≤ 0.01). (B to E) Growth curves of WT and PTS-deficient ( ptsH -H15A) S. aureus LAC in TSB under aerobic (B), anaerobic (C), NO-stressed (D), and metal-limiting (E) conditions ( n = 3). Abs, absorbance.

    Journal: mBio

    Article Title: Expanded Glucose Import Capability Affords Staphylococcus aureus Optimized Glycolytic Flux during Infection

    doi: 10.1128/mBio.00296-16

    Figure Lengend Snippet: PTS-dependent carbohydrate uptake contributes disproportionately to the nonrespiratory growth of S. aureus . (A) Glucose yield (milligrams of glucose consumed per milligram [dry weight] of biomass) of S. aureus COL under respiratory and nonrespiratory (anaerobic and NO-stressed) conditions ( n = 3; error bars show the standard error of the mean). S. aureus consumes ~3-fold more glucose per cell under nonrespiratory conditions. Statistical significance was calculated with a Student two-sided t test (*, P ≤ 0.01). (B to E) Growth curves of WT and PTS-deficient ( ptsH -H15A) S. aureus LAC in TSB under aerobic (B), anaerobic (C), NO-stressed (D), and metal-limiting (E) conditions ( n = 3). Abs, absorbance.

    Article Snippet: To test whether this enhanced growth behavior occurs under other nonrespiratory conditions, we compared the anaerobic growth of S. aureus strains COL and LAC to that of S. epidermidis RP62A, S. haemolyticus ATCC 29970, and S. saprophyticus ATCC 15305 in a rich medium.

    Techniques:

    S. aureus Encodes enhanced carbohydrate transport capability. Shown is a Venn diagram depicting the presence and conservation of putative carbohydrate transport proteins in the genomes of S. aureus COL, S. epidermidis RP62A, S. haemolyticus JCSC1435, and S. saprophyticus ATCC 15305. S. aureus encodes more overall transporters ( n = 22) and the highest number of unique transporters not found in any of the other species ( n = 10).

    Journal: mBio

    Article Title: Expanded Glucose Import Capability Affords Staphylococcus aureus Optimized Glycolytic Flux during Infection

    doi: 10.1128/mBio.00296-16

    Figure Lengend Snippet: S. aureus Encodes enhanced carbohydrate transport capability. Shown is a Venn diagram depicting the presence and conservation of putative carbohydrate transport proteins in the genomes of S. aureus COL, S. epidermidis RP62A, S. haemolyticus JCSC1435, and S. saprophyticus ATCC 15305. S. aureus encodes more overall transporters ( n = 22) and the highest number of unique transporters not found in any of the other species ( n = 10).

    Article Snippet: To test whether this enhanced growth behavior occurs under other nonrespiratory conditions, we compared the anaerobic growth of S. aureus strains COL and LAC to that of S. epidermidis RP62A, S. haemolyticus ATCC 29970, and S. saprophyticus ATCC 15305 in a rich medium.

    Techniques:

    S. aureus displays better anaerobic growth than CoNS. Anaerobic growth of S. aureus (SA) COL and LAC, S. epidermidis (SE) RP62A, S. haemolyticus (SH) ATCC 29970, and S. saprophyticus (SS) ATCC 15305 in TSB (A) and CDM plus 25 mM glucose (C) ( n = 3). Corresponding average growth rates for TSB and CDM plus glucose are displayed in panels B and D, respectively ( n = 3; error bars show the pooled standard error of the mean). Growth rates were calculated from 2 to 4 h ( S. aureus LAC) and 3 to 5 h ( S. aureus COL, S. epidermidis , S. haemolyticus , and S. saprophyticus ) in TSB and from 2 to 8 h in CDM plus 25 mM glucose. Statistical significance was calculated with a Student two-sided t test (***, P ≤ 0.001). Abs, absorbance.

    Journal: mBio

    Article Title: Expanded Glucose Import Capability Affords Staphylococcus aureus Optimized Glycolytic Flux during Infection

    doi: 10.1128/mBio.00296-16

    Figure Lengend Snippet: S. aureus displays better anaerobic growth than CoNS. Anaerobic growth of S. aureus (SA) COL and LAC, S. epidermidis (SE) RP62A, S. haemolyticus (SH) ATCC 29970, and S. saprophyticus (SS) ATCC 15305 in TSB (A) and CDM plus 25 mM glucose (C) ( n = 3). Corresponding average growth rates for TSB and CDM plus glucose are displayed in panels B and D, respectively ( n = 3; error bars show the pooled standard error of the mean). Growth rates were calculated from 2 to 4 h ( S. aureus LAC) and 3 to 5 h ( S. aureus COL, S. epidermidis , S. haemolyticus , and S. saprophyticus ) in TSB and from 2 to 8 h in CDM plus 25 mM glucose. Statistical significance was calculated with a Student two-sided t test (***, P ≤ 0.001). Abs, absorbance.

    Article Snippet: To test whether this enhanced growth behavior occurs under other nonrespiratory conditions, we compared the anaerobic growth of S. aureus strains COL and LAC to that of S. epidermidis RP62A, S. haemolyticus ATCC 29970, and S. saprophyticus ATCC 15305 in a rich medium.

    Techniques:

    Contributions of the identified glucose transporters to the nonrespiratory growth of S. aureus . (A) Aerobic growth of WT and selected double, triple, and quadruple S. aureus COL glucose transporter mutants in CDM plus 25 mM glucose ( n = 3). (B) Representative aerobic growth curve demonstrating complementation of S. aureus COL ΔG4 mutant growth in CDM plus 25 mM glucose by each individual glucose transporter ( n = 3). (C) Percent [U- 14 C]glucose uptake by S. aureus COL ΔG4 relative to that of the WT, as well as ΔG4 complemented with each individual glucose transporter gene. Uptake by each strain was measured following 12 min of incubation with radiolabeled substrate and then normalized to that of the WT ( n = 4; error bars show the standard error of the mean). Statistical significance was calculated with a Student two-sided t test (*, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001). (D and E) Nonrespiratory growth rate of S. aureus COL ΔG4, relative to that of the WT, compared to that of mutants expressing individual transporter genes from their native promoters. Strains were cultured anaerobically (D) or under NO stress (10 mM NOC-12–1 mM DEA-NO) (E) ( n = 3; error bars show the pooled standard error of the mean). Statistical significance was calculated with a Student two-sided t test (**, P ≤ 0.01; ***, P ≤ 0.001). Abs, absorbance.

    Journal: mBio

    Article Title: Expanded Glucose Import Capability Affords Staphylococcus aureus Optimized Glycolytic Flux during Infection

    doi: 10.1128/mBio.00296-16

    Figure Lengend Snippet: Contributions of the identified glucose transporters to the nonrespiratory growth of S. aureus . (A) Aerobic growth of WT and selected double, triple, and quadruple S. aureus COL glucose transporter mutants in CDM plus 25 mM glucose ( n = 3). (B) Representative aerobic growth curve demonstrating complementation of S. aureus COL ΔG4 mutant growth in CDM plus 25 mM glucose by each individual glucose transporter ( n = 3). (C) Percent [U- 14 C]glucose uptake by S. aureus COL ΔG4 relative to that of the WT, as well as ΔG4 complemented with each individual glucose transporter gene. Uptake by each strain was measured following 12 min of incubation with radiolabeled substrate and then normalized to that of the WT ( n = 4; error bars show the standard error of the mean). Statistical significance was calculated with a Student two-sided t test (*, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001). (D and E) Nonrespiratory growth rate of S. aureus COL ΔG4, relative to that of the WT, compared to that of mutants expressing individual transporter genes from their native promoters. Strains were cultured anaerobically (D) or under NO stress (10 mM NOC-12–1 mM DEA-NO) (E) ( n = 3; error bars show the pooled standard error of the mean). Statistical significance was calculated with a Student two-sided t test (**, P ≤ 0.01; ***, P ≤ 0.001). Abs, absorbance.

    Article Snippet: To test whether this enhanced growth behavior occurs under other nonrespiratory conditions, we compared the anaerobic growth of S. aureus strains COL and LAC to that of S. epidermidis RP62A, S. haemolyticus ATCC 29970, and S. saprophyticus ATCC 15305 in a rich medium.

    Techniques: Mutagenesis, Incubation, Expressing, Cell Culture

    Southern blot analysis of cfr in 1128105 and the RN4220 p1128105 transformant. Agarose gel electrophoresis of plasmid preparations (A) and Southern hybridization analysis using a cfr -specific probe (B) with S. aureus strains 1128105 (lanes 2 and 3), RN4220

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Linezolid-Resistant Staphylococcus aureus Strain 1128105, the First Known Clinical Isolate Possessing the cfr Multidrug Resistance Gene

    doi: 10.1128/AAC.03493-14

    Figure Lengend Snippet: Southern blot analysis of cfr in 1128105 and the RN4220 p1128105 transformant. Agarose gel electrophoresis of plasmid preparations (A) and Southern hybridization analysis using a cfr -specific probe (B) with S. aureus strains 1128105 (lanes 2 and 3), RN4220

    Article Snippet: S. aureus strains 1128105 (initially designated 3133832) , ATCC 29213, ATCC 29213 T2500A, RN4220, and transformants thereof were cultured aerobically at 37°C on cation-adjusted Mueller-Hinton II agar (MHA) (Becton Dickinson, Franklin Lakes, NJ) or in MH broth (MHB).

    Techniques: Southern Blot, Agarose Gel Electrophoresis, Plasmid Preparation, Hybridization

    Quantification (mean ± SD; n = 5) of viable S. aureus ATCC 15981 and V329 bacteria (CFU) found on infected sealed catheters precolonized with the correspondent S. aureus strain and implanted subcutaneously in mice. Catheters infected with strain ATCC 15981 (close squares) were naturally expelled between days 7 and 14 postimplantation (PI). At each interval, 1 of 5 catheters successfully precolonized in vitro with strain V329 became uninfected after subcutaneous implantation in vivo , and the corresponding data have not been included in the figure. Statistical comparisons by Fisher's LSD test showed that P values of

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: In Vivo Monitoring of Staphylococcus aureus Biofilm Infections and Antimicrobial Therapy by [18F]Fluoro-Deoxyglucose–MicroPET in a Mouse Model

    doi: 10.1128/AAC.03138-14

    Figure Lengend Snippet: Quantification (mean ± SD; n = 5) of viable S. aureus ATCC 15981 and V329 bacteria (CFU) found on infected sealed catheters precolonized with the correspondent S. aureus strain and implanted subcutaneously in mice. Catheters infected with strain ATCC 15981 (close squares) were naturally expelled between days 7 and 14 postimplantation (PI). At each interval, 1 of 5 catheters successfully precolonized in vitro with strain V329 became uninfected after subcutaneous implantation in vivo , and the corresponding data have not been included in the figure. Statistical comparisons by Fisher's LSD test showed that P values of

    Article Snippet: As expected from the nature of the biofilm matrix and the specificity of the anti-matrix-polymer antibodies used, the anti-Bap and anti-PIA/PNAG antibodies stained specifically free bacteria, bacterial aggregates, and neutrophil-phagocytized bacterial antigen in S. aureus strain V329 and ATCC 15981 infections, respectively ( and ).

    Techniques: Infection, Mouse Assay, In Vitro, In Vivo

    Representative [ 18 F]FDG-MicroPET images in mice carrying catheters infected with S. aureus strains ATCC 15981 or V329. Slices at dorsal (A) and ventral (B) positions show [ 18 F]-FGD uptake in catheter (arrows) or axillary lymph node (ALN; arrowheads) areas, respectively. Brain (b), heart (h), bladder (bl), and spinal cord (sp) show physiological uptake of [ 18 F]-FGD. In panels A and B, each image corresponds to a representative animal analyzed at days 1, 7, and 14 PI. Images of uninfected controls (receiving only TSB-glc) are included on the left. (C) Box plots illustrating the evolution of the SUV60 index in S. aureus ATCC 15981 or V329 infections in the catheter area (left panel) and SUVmax in ALN (right panel). Statistical comparisons by Fisher's PLSD test: *, P

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: In Vivo Monitoring of Staphylococcus aureus Biofilm Infections and Antimicrobial Therapy by [18F]Fluoro-Deoxyglucose–MicroPET in a Mouse Model

    doi: 10.1128/AAC.03138-14

    Figure Lengend Snippet: Representative [ 18 F]FDG-MicroPET images in mice carrying catheters infected with S. aureus strains ATCC 15981 or V329. Slices at dorsal (A) and ventral (B) positions show [ 18 F]-FGD uptake in catheter (arrows) or axillary lymph node (ALN; arrowheads) areas, respectively. Brain (b), heart (h), bladder (bl), and spinal cord (sp) show physiological uptake of [ 18 F]-FGD. In panels A and B, each image corresponds to a representative animal analyzed at days 1, 7, and 14 PI. Images of uninfected controls (receiving only TSB-glc) are included on the left. (C) Box plots illustrating the evolution of the SUV60 index in S. aureus ATCC 15981 or V329 infections in the catheter area (left panel) and SUVmax in ALN (right panel). Statistical comparisons by Fisher's PLSD test: *, P

    Article Snippet: As expected from the nature of the biofilm matrix and the specificity of the anti-matrix-polymer antibodies used, the anti-Bap and anti-PIA/PNAG antibodies stained specifically free bacteria, bacterial aggregates, and neutrophil-phagocytized bacterial antigen in S. aureus strain V329 and ATCC 15981 infections, respectively ( and ).

    Techniques: Mouse Assay, Infection, Gas Chromatography

    Design of the four mouse assays (A to D) performed in this work, according to animal group distribution and time points for analysis (indicated with arrows). (A) Bacteriological study on the evolution of infections (log 10 CFU/catheter); (B) histological changes in subcutaneous tissue during infection, using hematoxylin and eosin (H E) and immunohistochemical (IHC) staining; (C) in vivo [ 18 F]FDG-MicroPET imaging in an infection follow-up study; (D) [ 18 F]FDG-MicroPET imaging and bacteriological study (log 10 CFU/catheter) in mice carrying infected catheters and then treated (T) daily for 1 week with rifampin (0.5 mg/day/mouse) or receiving PBS (untreated controls). Experiments illustrated in panels A, B, and C were carried out each independently with strains S. aureus ATCC 15981 and V329 and also included animal groups with uninfected catheters (control groups). The experiment shown in panel D was done with strain ATCC 15981.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: In Vivo Monitoring of Staphylococcus aureus Biofilm Infections and Antimicrobial Therapy by [18F]Fluoro-Deoxyglucose–MicroPET in a Mouse Model

    doi: 10.1128/AAC.03138-14

    Figure Lengend Snippet: Design of the four mouse assays (A to D) performed in this work, according to animal group distribution and time points for analysis (indicated with arrows). (A) Bacteriological study on the evolution of infections (log 10 CFU/catheter); (B) histological changes in subcutaneous tissue during infection, using hematoxylin and eosin (H E) and immunohistochemical (IHC) staining; (C) in vivo [ 18 F]FDG-MicroPET imaging in an infection follow-up study; (D) [ 18 F]FDG-MicroPET imaging and bacteriological study (log 10 CFU/catheter) in mice carrying infected catheters and then treated (T) daily for 1 week with rifampin (0.5 mg/day/mouse) or receiving PBS (untreated controls). Experiments illustrated in panels A, B, and C were carried out each independently with strains S. aureus ATCC 15981 and V329 and also included animal groups with uninfected catheters (control groups). The experiment shown in panel D was done with strain ATCC 15981.

    Article Snippet: As expected from the nature of the biofilm matrix and the specificity of the anti-matrix-polymer antibodies used, the anti-Bap and anti-PIA/PNAG antibodies stained specifically free bacteria, bacterial aggregates, and neutrophil-phagocytized bacterial antigen in S. aureus strain V329 and ATCC 15981 infections, respectively ( and ).

    Techniques: Infection, Immunohistochemistry, Staining, In Vivo, Imaging, Mouse Assay

    Susceptibility of the clinical  S. aureus  strains to the geranium oil.

    Journal: Molecules

    Article Title: Antimicrobial Activity of Geranium Oil against Clinical Strains of Staphylococcus aureus

    doi: 10.3390/molecules170910276

    Figure Lengend Snippet: Susceptibility of the clinical S. aureus strains to the geranium oil.

    Article Snippet: Susceptibility of the Clinical S. aureus Strains to the Geranium Oil The geranium oil obtained from Pelargonium graveolens Ait. shows a very strong activity against the standard S. aureus strain (ATCC 433000) and also against the examined S. aureus strains obtained from the clinical materials.

    Techniques:

    Opsonophagocytic killing of several staphylococcal strains with MAb F598 at 12 μg/ml. Bars represent means; error bars represent the standard errors of the means. S. aureus strains Mn8, Newman, 10833, and Reynolds and S. epidermidis strain M187

    Journal:

    Article Title: Characterization of the Opsonic and Protective Activity against Staphylococcus aureus of Fully Human Monoclonal Antibodies Specific for the Bacterial Surface Polysaccharide Poly-N-Acetylglucosamine

    doi: 10.1128/IAI.74.5.2742-2750.2006

    Figure Lengend Snippet: Opsonophagocytic killing of several staphylococcal strains with MAb F598 at 12 μg/ml. Bars represent means; error bars represent the standard errors of the means. S. aureus strains Mn8, Newman, 10833, and Reynolds and S. epidermidis strain M187

    Article Snippet: S. aureus strains MN8 (capsular type 8 [CP8]), NCTC 10833 (ATCC 25904; CP untypable), Reynolds (CP5), and Newman (CP5) and S. epidermidis strain M187 were obtained and propagated as previously described ( ).

    Techniques:

    Immunofluorescence study of binding of MAbs F598 and F628 to wild-type (WT) and Δ ica S. aureus strain Mn8, showing specificity of binding to the PNAG-producing parental strain.

    Journal:

    Article Title: Characterization of the Opsonic and Protective Activity against Staphylococcus aureus of Fully Human Monoclonal Antibodies Specific for the Bacterial Surface Polysaccharide Poly-N-Acetylglucosamine

    doi: 10.1128/IAI.74.5.2742-2750.2006

    Figure Lengend Snippet: Immunofluorescence study of binding of MAbs F598 and F628 to wild-type (WT) and Δ ica S. aureus strain Mn8, showing specificity of binding to the PNAG-producing parental strain.

    Article Snippet: S. aureus strains MN8 (capsular type 8 [CP8]), NCTC 10833 (ATCC 25904; CP untypable), Reynolds (CP5), and Newman (CP5) and S. epidermidis strain M187 were obtained and propagated as previously described ( ).

    Techniques: Immunofluorescence, Binding Assay

    Opsonophagocytic activity of IgG1 and IgG2 MAbs. Target strain is S. aureus Mn8. Bars represent means; error bars represent the standard errors of the means for three to eight replicates. Percent reduction in CFU was calculated compared to controls containing

    Journal:

    Article Title: Characterization of the Opsonic and Protective Activity against Staphylococcus aureus of Fully Human Monoclonal Antibodies Specific for the Bacterial Surface Polysaccharide Poly-N-Acetylglucosamine

    doi: 10.1128/IAI.74.5.2742-2750.2006

    Figure Lengend Snippet: Opsonophagocytic activity of IgG1 and IgG2 MAbs. Target strain is S. aureus Mn8. Bars represent means; error bars represent the standard errors of the means for three to eight replicates. Percent reduction in CFU was calculated compared to controls containing

    Article Snippet: S. aureus strains MN8 (capsular type 8 [CP8]), NCTC 10833 (ATCC 25904; CP untypable), Reynolds (CP5), and Newman (CP5) and S. epidermidis strain M187 were obtained and propagated as previously described ( ).

    Techniques: Activity Assay

    Protection against S. aureus MN8 lethal infection with IgG1 MAbs to PNAG (eight mice per group). (A) A 400-μg volume of each MAb was administered 4 h before challenge. Strain Mn8 challenge dose, 5 × 10 8 . (B) A 200-μg volume of

    Journal:

    Article Title: Characterization of the Opsonic and Protective Activity against Staphylococcus aureus of Fully Human Monoclonal Antibodies Specific for the Bacterial Surface Polysaccharide Poly-N-Acetylglucosamine

    doi: 10.1128/IAI.74.5.2742-2750.2006

    Figure Lengend Snippet: Protection against S. aureus MN8 lethal infection with IgG1 MAbs to PNAG (eight mice per group). (A) A 400-μg volume of each MAb was administered 4 h before challenge. Strain Mn8 challenge dose, 5 × 10 8 . (B) A 200-μg volume of

    Article Snippet: S. aureus strains MN8 (capsular type 8 [CP8]), NCTC 10833 (ATCC 25904; CP untypable), Reynolds (CP5), and Newman (CP5) and S. epidermidis strain M187 were obtained and propagated as previously described ( ).

    Techniques: Infection, Mouse Assay

    Comparison of the orders of the genes that surround the chromosomal replication origins of S. carnosus and S. aureus N315. Sections of the oriC regions of S. aureus and S. carnosus illustrating the gene inversion observed in the staphylococcal genomes

    Journal: Applied and Environmental Microbiology

    Article Title: Genome Analysis of the Meat Starter Culture Bacterium Staphylococcus carnosus TM300 ▿ TM300 ▿ †

    doi: 10.1128/AEM.01982-08

    Figure Lengend Snippet: Comparison of the orders of the genes that surround the chromosomal replication origins of S. carnosus and S. aureus N315. Sections of the oriC regions of S. aureus and S. carnosus illustrating the gene inversion observed in the staphylococcal genomes

    Article Snippet: Based on BlastP analysis, we compared the derived gene products of S. carnosus with the proteins encoded by S. aureus strains N315, Mu50, MW2, COL, MSSA476, MRSA252, and RF122; S. epidermidis strains RP62A and ATCC 12228; S. haemolyticus JCSC 1435; and S. saprophyticus ATCC 15305.

    Techniques:

    Relative virulence factor production by S. aureus strains. (A) Basal level of virulence factor mRNA levels in a panel of S. aureus strains. The expression level in strain PDJ22 (Δ saeR ) was then set to 1, and the expression levels in the indicated strains were compared to the levels in the saeR -knockout strain. The differences between strains PDJ22 and USA300 were not significant. Strains Wood46, RN4220, and Newman exhibited significantly higher levels of virulence factor mRNA than did strains PDJ22 and USA300. *, P

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Perturbation of Staphylococcus aureus Gene Expression by the Enoyl-Acyl Carrier Protein Reductase Inhibitor AFN-1252

    doi: 10.1128/AAC.02307-12

    Figure Lengend Snippet: Relative virulence factor production by S. aureus strains. (A) Basal level of virulence factor mRNA levels in a panel of S. aureus strains. The expression level in strain PDJ22 (Δ saeR ) was then set to 1, and the expression levels in the indicated strains were compared to the levels in the saeR -knockout strain. The differences between strains PDJ22 and USA300 were not significant. Strains Wood46, RN4220, and Newman exhibited significantly higher levels of virulence factor mRNA than did strains PDJ22 and USA300. *, P

    Article Snippet: S. aureus strain RN4220 was obtained from the American Type Culture Collection.

    Techniques: Expressing, Knock-Out

    The binding of epidermis-derived IgG ( A ) and IgA ( B ) to E. coli strains DH10B and BL21, S. aureus strains Cowan I and ATCC 25923, clinical isolated strain of S. epidermidis , and C. albicans strain SC5314 was analyzed by ELISA. ( A ) Binding of IgG ( a ) and IgA ( b ) in epidermis tissue lysates from five healthy donors to microbes was analyzed by ELISA. 50μg/mL microbes were coated for testing Ig binding, vehicle as negative control. Data are presented as mean ± SD. Student’s t -tests were performed when mean of microbes-coated group was higher than vehicle control. Statistical significance: ns means no significant difference, * p

    Journal: International Journal of Molecular Sciences

    Article Title: IgG and IgA with Potential Microbial-Binding Activity Are Expressed by Normal Human Skin Epidermal Cells

    doi: 10.3390/ijms16022574

    Figure Lengend Snippet: The binding of epidermis-derived IgG ( A ) and IgA ( B ) to E. coli strains DH10B and BL21, S. aureus strains Cowan I and ATCC 25923, clinical isolated strain of S. epidermidis , and C. albicans strain SC5314 was analyzed by ELISA. ( A ) Binding of IgG ( a ) and IgA ( b ) in epidermis tissue lysates from five healthy donors to microbes was analyzed by ELISA. 50μg/mL microbes were coated for testing Ig binding, vehicle as negative control. Data are presented as mean ± SD. Student’s t -tests were performed when mean of microbes-coated group was higher than vehicle control. Statistical significance: ns means no significant difference, * p

    Article Snippet: We tested the binding of IgG and IgA extracted from epidermis of five donors to E. coli strains DH10B and BL21, S. aureus strains Cowan I and ATCC 25923, a clinical isolate of S. epidermidis , and Candida albicans (C. albicans ) strain SC5314, by ELISA.

    Techniques: Binding Assay, Derivative Assay, Isolation, Enzyme-linked Immunosorbent Assay, Negative Control

    Lysostaphin did not disrupt biofilms formed by lysostaphin-resistant S. aureus variants. Tissue culture wells (three for each sample) of a microtiter plate were inoculated with ∼10 8 CFU of S. aureus strain SA113, SA5 LysoR, MBT 5040, or MBT 5040 LysoR (as indicated). After we allowed 48 h for biofilm formation, the wells were washed twice and then incubated with (+) or without (−) 50 μg of lysostaphin/ml in PBS for 3 h. Following incubation, the wells were washed again and stained with safranin to visualize biofilms.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Lysostaphin Disrupts Staphylococcus aureus and Staphylococcus epidermidis Biofilms on Artificial Surfaces

    doi: 10.1128/AAC.47.11.3407-3414.2003

    Figure Lengend Snippet: Lysostaphin did not disrupt biofilms formed by lysostaphin-resistant S. aureus variants. Tissue culture wells (three for each sample) of a microtiter plate were inoculated with ∼10 8 CFU of S. aureus strain SA113, SA5 LysoR, MBT 5040, or MBT 5040 LysoR (as indicated). After we allowed 48 h for biofilm formation, the wells were washed twice and then incubated with (+) or without (−) 50 μg of lysostaphin/ml in PBS for 3 h. Following incubation, the wells were washed again and stained with safranin to visualize biofilms.

    Article Snippet: In similar polycarbonate transwell experiments observed by SEM, the biofilms produced by S. aureus strains MBT 5040, Col, and ATCC 35556 were also found to be eradicated; i.e., no sessile cells or glycocalyx remained after treatment with 100 μg of lysostaphin/ml in all wells examined (data not shown).

    Techniques: Incubation, Staining

    Lysostaphin-disrupted biofilms of MRSA strains Col and MBT 5040. Ninety-six-well polystyrene tissue culture wells (four for each sample) were inoculated with ∼10 8 CFU of S. aureus strain Col or MBT 5040 (as indicated). After we allowed 48 h for biofilm formation, the wells were washed twice and then incubated with (+) or without (−) 50 μg of lysostaphin/ml in PBS for 3 h. Following incubation, the wells were washed again and stained with safranin to visualize biofilms.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Lysostaphin Disrupts Staphylococcus aureus and Staphylococcus epidermidis Biofilms on Artificial Surfaces

    doi: 10.1128/AAC.47.11.3407-3414.2003

    Figure Lengend Snippet: Lysostaphin-disrupted biofilms of MRSA strains Col and MBT 5040. Ninety-six-well polystyrene tissue culture wells (four for each sample) were inoculated with ∼10 8 CFU of S. aureus strain Col or MBT 5040 (as indicated). After we allowed 48 h for biofilm formation, the wells were washed twice and then incubated with (+) or without (−) 50 μg of lysostaphin/ml in PBS for 3 h. Following incubation, the wells were washed again and stained with safranin to visualize biofilms.

    Article Snippet: In similar polycarbonate transwell experiments observed by SEM, the biofilms produced by S. aureus strains MBT 5040, Col, and ATCC 35556 were also found to be eradicated; i.e., no sessile cells or glycocalyx remained after treatment with 100 μg of lysostaphin/ml in all wells examined (data not shown).

    Techniques: Incubation, Staining

    Binding and activity of CF-301. (A) Effects of serum and medium pretreatments on CF-301 binding to S. aureus MW2 determined by differential interference contrast (DIC) and fluorescence microscopy. Exposures of 3 ms (human, rabbit, and mouse plus HSA samples) and 3 s (rat, mouse, and caMHB samples) were used to visualize CF-301 RHOD . MIC values for each condition are indicated (micrograms per milliliter). (B) Effect of HSA on CF-301 binding to S. aureus ATCC 700699 determined by deconvolution microscopy. Maximum-intensity projections are shown. CF-301 RHOD , red; DAPI, blue (scale bar, 2 μm). (C) Effect of CF-301 on HuLYZ binding to S. aureus ATCC 700699 determined by deconvolution microscopy. Maximum-intensity projections are shown. HuLYZ AF , green; DAPI, blue (scale bar, 4 μm). (D) Bactericidal activity of CF-301 in human serum and caMHB determined by TEM. CF-301 was tested at 10×, 1×, and 0.1× MIC levels for each environment. Direct magnification values: control, ×13,000; 10× MIC, ×2,600; 1× and 0.1× MIC, ×6,600. Scale bars, 1 μm.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: The Antistaphylococcal Lysin, CF-301, Activates Key Host Factors in Human Blood To Potentiate Methicillin-Resistant Staphylococcus aureus Bacteriolysis

    doi: 10.1128/AAC.02291-18

    Figure Lengend Snippet: Binding and activity of CF-301. (A) Effects of serum and medium pretreatments on CF-301 binding to S. aureus MW2 determined by differential interference contrast (DIC) and fluorescence microscopy. Exposures of 3 ms (human, rabbit, and mouse plus HSA samples) and 3 s (rat, mouse, and caMHB samples) were used to visualize CF-301 RHOD . MIC values for each condition are indicated (micrograms per milliliter). (B) Effect of HSA on CF-301 binding to S. aureus ATCC 700699 determined by deconvolution microscopy. Maximum-intensity projections are shown. CF-301 RHOD , red; DAPI, blue (scale bar, 2 μm). (C) Effect of CF-301 on HuLYZ binding to S. aureus ATCC 700699 determined by deconvolution microscopy. Maximum-intensity projections are shown. HuLYZ AF , green; DAPI, blue (scale bar, 4 μm). (D) Bactericidal activity of CF-301 in human serum and caMHB determined by TEM. CF-301 was tested at 10×, 1×, and 0.1× MIC levels for each environment. Direct magnification values: control, ×13,000; 10× MIC, ×2,600; 1× and 0.1× MIC, ×6,600. Scale bars, 1 μm.

    Article Snippet: The impact of pretreatment with HSA (at 40 mg/ml) on the surface labeling of S. aureus strain ATCC 700699 with CF-301RHOD was next examined by deconvolution microscopy.

    Techniques: Binding Assay, Activity Assay, Fluorescence, Microscopy, Mass Spectrometry, Transmission Electron Microscopy

    SEM images of S. aureus ATCC 700699 cells left untreated (A) or treated with polymyxin B (PMB; 99.5 mg/liter) (B), FADDI-019 (1× MIC) (C), FADDI-019 (4× MIC) (D), and FADDI-019 (8× MIC) (E) for 1 h. The white scale bar in each figure represents 500 nm.

    Journal: mSphere

    Article Title: Transcriptomic Analysis of the Activity of a Novel Polymyxin against Staphylococcus aureus

    doi: 10.1128/mSphere.00119-16

    Figure Lengend Snippet: SEM images of S. aureus ATCC 700699 cells left untreated (A) or treated with polymyxin B (PMB; 99.5 mg/liter) (B), FADDI-019 (1× MIC) (C), FADDI-019 (4× MIC) (D), and FADDI-019 (8× MIC) (E) for 1 h. The white scale bar in each figure represents 500 nm.

    Article Snippet: The MICs of FADDI-019 against S. aureus strains ATCC 700699 and ATCC 700698 were both 16 mg/liter, consistent with our previous report ( ).

    Techniques:

    Time-kill kinetics of FADDI-019 against S. aureus ATCC 700699. The error bars show the standard deviations of the results from three independent biological repeats.

    Journal: mSphere

    Article Title: Transcriptomic Analysis of the Activity of a Novel Polymyxin against Staphylococcus aureus

    doi: 10.1128/mSphere.00119-16

    Figure Lengend Snippet: Time-kill kinetics of FADDI-019 against S. aureus ATCC 700699. The error bars show the standard deviations of the results from three independent biological repeats.

    Article Snippet: The MICs of FADDI-019 against S. aureus strains ATCC 700699 and ATCC 700698 were both 16 mg/liter, consistent with our previous report ( ).

    Techniques: