s. aureus sa113 Search Results


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  • 95
    ATCC s aureus pcx19ωhemb s aureus sa113
    S Aureus Pcx19ωhemb S Aureus Sa113, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s aureus pcx19ωhemb s aureus sa113/product/ATCC
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    99
    Millipore s aureus sa113
    Expression of IsdA and IsdB by S. aureus <t>SA113</t> after PGG treatment. S. aureus SA113 cells were cultured in TSBg (A), TSBg-PGG (B) medium, or TSBg-PGG medium containing 100 µM FeSO 4 (C) for 24 h. Proteins extracted from bacterial surface were analyzed by two-dimensional gel electrophoresis and by silver staining. The IsdA and IsdB spots (circles) were identified by MALDI-TOF spectrometry.
    S Aureus Sa113, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s aureus sa113/product/Millipore
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    95
    ATCC s aureus sa113
    Effects of PGG on bacterial growth and biofilm formation. (A) S. aureus <t>SA113</t> was cultured in TSBg broth that contains PGG in 96-well microtiter plates at 37°C for 6 h (▪) and 24 h (□). The cell density was determined at A 578 .
    S Aureus Sa113, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s aureus sa113/product/ATCC
    Average 95 stars, based on 68 article reviews
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    96
    ATCC chemicals s aureus sa113
    Effects of PGG on bacterial growth and biofilm formation. (A) S. aureus <t>SA113</t> was cultured in TSBg broth that contains PGG in 96-well microtiter plates at 37°C for 6 h (▪) and 24 h (□). The cell density was determined at A 578 .
    Chemicals S Aureus Sa113, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Inserm Transfert s aureus sa113 δ spa
    Alpha-toxin is not sufficient to disrupt phagolysosomal membranes. (A) Alpha-toxin immunofluorescence analyses demonstrate that staphylococci (green, S. carnosus ; blue, S. aureus <t>SA113</t> <t>Δ</t> spa ) expressing alpha-toxin (red) are found exclusively within
    S Aureus Sa113 δ Spa, supplied by Inserm Transfert, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Expression of IsdA and IsdB by S. aureus SA113 after PGG treatment. S. aureus SA113 cells were cultured in TSBg (A), TSBg-PGG (B) medium, or TSBg-PGG medium containing 100 µM FeSO 4 (C) for 24 h. Proteins extracted from bacterial surface were analyzed by two-dimensional gel electrophoresis and by silver staining. The IsdA and IsdB spots (circles) were identified by MALDI-TOF spectrometry.

    Journal: PLoS ONE

    Article Title: Involvement of Iron in Biofilm Formation by Staphylococcus aureus

    doi: 10.1371/journal.pone.0034388

    Figure Lengend Snippet: Expression of IsdA and IsdB by S. aureus SA113 after PGG treatment. S. aureus SA113 cells were cultured in TSBg (A), TSBg-PGG (B) medium, or TSBg-PGG medium containing 100 µM FeSO 4 (C) for 24 h. Proteins extracted from bacterial surface were analyzed by two-dimensional gel electrophoresis and by silver staining. The IsdA and IsdB spots (circles) were identified by MALDI-TOF spectrometry.

    Article Snippet: Detection of PIA The PIA extracted from S. aureus SA113 was blotted onto PVDF membrane (Millipore, Billerica, MA) using a 96-well dot-blot apparatus according to a method described elsewhere .

    Techniques: Expressing, Cell Culture, Two-Dimensional Gel Electrophoresis, Electrophoresis, Silver Staining

    Effect of iron on biofilm formation. S. aureus SA113 was cultured in TSBg, TSBg-PGG and TSBg-PGG medium containing FeSO 4 in wells in a 96-well microtiter plate. After incubation at 37°C for 24 h, biofilm formation in the well was measured by safranin-staining method (A). The amount of biofilm formed by the control cells that was treated with DMSO was set to 100%. Biofilm formation is presented as percentage of that in the control cells. Experiments were performed three times, and each sample in the experiment was prepared in six wells. Error bars represent standard error. (B) Cells were cultured in TSBg, TSBg-PGG, and TSBg-PGG containing 100 µM FeSO 4 . After incubation for 24 h, the biofilm that had formed on the coverslips was stained using a LIVE/DEAD Bac Light Bacterial Viability kit (Invitrogen). Cells not treated with PGG were used as the control. The biofilm structure was examined under a confocal laser-scanning microscope. (a–c) Images reconstructed from a series of Z sections. (d–f) Images reconstructed from average intensity projection through confocal image stacks of series of X-Z (bottom) and Y-Z (right) sections. The number at the bottom represents the thickness (µm) of the biofilm. The bar represents 25 µm.

    Journal: PLoS ONE

    Article Title: Involvement of Iron in Biofilm Formation by Staphylococcus aureus

    doi: 10.1371/journal.pone.0034388

    Figure Lengend Snippet: Effect of iron on biofilm formation. S. aureus SA113 was cultured in TSBg, TSBg-PGG and TSBg-PGG medium containing FeSO 4 in wells in a 96-well microtiter plate. After incubation at 37°C for 24 h, biofilm formation in the well was measured by safranin-staining method (A). The amount of biofilm formed by the control cells that was treated with DMSO was set to 100%. Biofilm formation is presented as percentage of that in the control cells. Experiments were performed three times, and each sample in the experiment was prepared in six wells. Error bars represent standard error. (B) Cells were cultured in TSBg, TSBg-PGG, and TSBg-PGG containing 100 µM FeSO 4 . After incubation for 24 h, the biofilm that had formed on the coverslips was stained using a LIVE/DEAD Bac Light Bacterial Viability kit (Invitrogen). Cells not treated with PGG were used as the control. The biofilm structure was examined under a confocal laser-scanning microscope. (a–c) Images reconstructed from a series of Z sections. (d–f) Images reconstructed from average intensity projection through confocal image stacks of series of X-Z (bottom) and Y-Z (right) sections. The number at the bottom represents the thickness (µm) of the biofilm. The bar represents 25 µm.

    Article Snippet: Detection of PIA The PIA extracted from S. aureus SA113 was blotted onto PVDF membrane (Millipore, Billerica, MA) using a 96-well dot-blot apparatus according to a method described elsewhere .

    Techniques: Cell Culture, Incubation, Staining, BAC Assay, Laser-Scanning Microscopy

    Iron restores biofilm formation in iron-restricted medium. S. aureus SA113 was cultured in BM medium that contained PGG (A, B, C) or 2-DP (D, E, F) in wells in a 96-well microtiter plate. Following incubation at 37°C for 24 h, the cell density was determined at A578 (A, D). The amount of biofilm formation in the well was determined at A490 after safranin staining (B, C, D, E). After FeSO4 was added to BM medium that contained 12.5 µM PGG (C) or 1 mM 2-DP (F) and incubated at 37°C for 24 h, the biofilm that was formed in the well was washed and stained by safranin. The cell density and biofilm formation by the cells that were treated with either DMSO or distilled water were used as controls and set to 100%. Experiments were performed three times, and each sample in each experiment was prepared in six wells. The error bar represents the standard error.

    Journal: PLoS ONE

    Article Title: Involvement of Iron in Biofilm Formation by Staphylococcus aureus

    doi: 10.1371/journal.pone.0034388

    Figure Lengend Snippet: Iron restores biofilm formation in iron-restricted medium. S. aureus SA113 was cultured in BM medium that contained PGG (A, B, C) or 2-DP (D, E, F) in wells in a 96-well microtiter plate. Following incubation at 37°C for 24 h, the cell density was determined at A578 (A, D). The amount of biofilm formation in the well was determined at A490 after safranin staining (B, C, D, E). After FeSO4 was added to BM medium that contained 12.5 µM PGG (C) or 1 mM 2-DP (F) and incubated at 37°C for 24 h, the biofilm that was formed in the well was washed and stained by safranin. The cell density and biofilm formation by the cells that were treated with either DMSO or distilled water were used as controls and set to 100%. Experiments were performed three times, and each sample in each experiment was prepared in six wells. The error bar represents the standard error.

    Article Snippet: Detection of PIA The PIA extracted from S. aureus SA113 was blotted onto PVDF membrane (Millipore, Billerica, MA) using a 96-well dot-blot apparatus according to a method described elsewhere .

    Techniques: Cell Culture, Incubation, Staining

    Effect of iron on adherence of S. aureus to solid surfaces and PIA synthesis. S. aureus SA113 (A) and clinical strains, i.e. , SA130, SA148, SA229, SA435 (C) were inoculated in TSBg that contained 0 µM and 25 µM of PGG (TSBg-PGG) or supplemented with FeSO 4 in 9-cm petri dishes. After 24 h incubation, biofilm that formed on the plate was photographed. The plates were tilted and photographed to show the attachment of cells to the petri plate and the clumping of cells in the medium in the lower half of the plate. (B) PIA was extracted from S. aureus SA113 that had been cultured for 24 h in TSBg or TSBg-PGG medium containing 0, 50 and 100 µM FeSO 4 . PIA was detected using WGA-biotin. After incubation with HRP-streptavidin, the spots were visualized by chemiluminescence detection.

    Journal: PLoS ONE

    Article Title: Involvement of Iron in Biofilm Formation by Staphylococcus aureus

    doi: 10.1371/journal.pone.0034388

    Figure Lengend Snippet: Effect of iron on adherence of S. aureus to solid surfaces and PIA synthesis. S. aureus SA113 (A) and clinical strains, i.e. , SA130, SA148, SA229, SA435 (C) were inoculated in TSBg that contained 0 µM and 25 µM of PGG (TSBg-PGG) or supplemented with FeSO 4 in 9-cm petri dishes. After 24 h incubation, biofilm that formed on the plate was photographed. The plates were tilted and photographed to show the attachment of cells to the petri plate and the clumping of cells in the medium in the lower half of the plate. (B) PIA was extracted from S. aureus SA113 that had been cultured for 24 h in TSBg or TSBg-PGG medium containing 0, 50 and 100 µM FeSO 4 . PIA was detected using WGA-biotin. After incubation with HRP-streptavidin, the spots were visualized by chemiluminescence detection.

    Article Snippet: Detection of PIA The PIA extracted from S. aureus SA113 was blotted onto PVDF membrane (Millipore, Billerica, MA) using a 96-well dot-blot apparatus according to a method described elsewhere .

    Techniques: Incubation, Cell Culture, Whole Genome Amplification

    Effects of PGG on bacterial growth and biofilm formation. (A) S. aureus SA113 was cultured in TSBg broth that contains PGG in 96-well microtiter plates at 37°C for 6 h (▪) and 24 h (□). The cell density was determined at A 578 .

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Inhibitory Effects of 1,2,3,4,6-Penta-O-Galloyl-?-d-Glucopyranose on Biofilm Formation by Staphylococcus aureus ▿ ▿ †

    doi: 10.1128/AAC.00843-10

    Figure Lengend Snippet: Effects of PGG on bacterial growth and biofilm formation. (A) S. aureus SA113 was cultured in TSBg broth that contains PGG in 96-well microtiter plates at 37°C for 6 h (▪) and 24 h (□). The cell density was determined at A 578 .

    Article Snippet: A total of 48 compounds isolated from plants commonly used in Chinese medicine were screened for their activity to prevent biofilm formation by S. aureus SA113 at 6 h after seeding the bacteria in a 96-well polystyrene microtiter plate.

    Techniques: Cell Culture

    Kinetics of the antiadherence activity of PGG. (A) S. aureus SA113 was seeded in a 96-well microtiter plate. PGG at 6.25 μM (▪) and 12.5 μM (□) was added to the culturing medium at 0, 0.5, 1.0, 1.5, and 2 h after inoculation.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Inhibitory Effects of 1,2,3,4,6-Penta-O-Galloyl-?-d-Glucopyranose on Biofilm Formation by Staphylococcus aureus ▿ ▿ †

    doi: 10.1128/AAC.00843-10

    Figure Lengend Snippet: Kinetics of the antiadherence activity of PGG. (A) S. aureus SA113 was seeded in a 96-well microtiter plate. PGG at 6.25 μM (▪) and 12.5 μM (□) was added to the culturing medium at 0, 0.5, 1.0, 1.5, and 2 h after inoculation.

    Article Snippet: A total of 48 compounds isolated from plants commonly used in Chinese medicine were screened for their activity to prevent biofilm formation by S. aureus SA113 at 6 h after seeding the bacteria in a 96-well polystyrene microtiter plate.

    Techniques: Activity Assay

    Inhibition of PIA expression by PGG. SEM images of S. aureus SA113 cultured on polycarbonate discs in the absence (A) or presence (B) of 3.13 μM PGG for 6 h. The images shown were taken at a magnification of ×30,000. Bar, 1.0 μm.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Inhibitory Effects of 1,2,3,4,6-Penta-O-Galloyl-?-d-Glucopyranose on Biofilm Formation by Staphylococcus aureus ▿ ▿ †

    doi: 10.1128/AAC.00843-10

    Figure Lengend Snippet: Inhibition of PIA expression by PGG. SEM images of S. aureus SA113 cultured on polycarbonate discs in the absence (A) or presence (B) of 3.13 μM PGG for 6 h. The images shown were taken at a magnification of ×30,000. Bar, 1.0 μm.

    Article Snippet: A total of 48 compounds isolated from plants commonly used in Chinese medicine were screened for their activity to prevent biofilm formation by S. aureus SA113 at 6 h after seeding the bacteria in a 96-well polystyrene microtiter plate.

    Techniques: Inhibition, Expressing, Cell Culture

    Alpha-toxin is not sufficient to disrupt phagolysosomal membranes. (A) Alpha-toxin immunofluorescence analyses demonstrate that staphylococci (green, S. carnosus ; blue, S. aureus SA113 Δ spa ) expressing alpha-toxin (red) are found exclusively within

    Journal: Infection and Immunity

    Article Title: Staphylococcal Alpha-Toxin Is Not Sufficient To Mediate Escape from Phagolysosomes in Upper-Airway Epithelial Cells

    doi: 10.1128/IAI.01478-08

    Figure Lengend Snippet: Alpha-toxin is not sufficient to disrupt phagolysosomal membranes. (A) Alpha-toxin immunofluorescence analyses demonstrate that staphylococci (green, S. carnosus ; blue, S. aureus SA113 Δ spa ) expressing alpha-toxin (red) are found exclusively within

    Article Snippet: We are indebted to following colleagues: Ambrose Cheung (Dartmouth, NH) for pALC2084, Susanne Engelmann (Greifswald, Germany) for purified alpha-toxin as well as S. aureus strains GP269 and RN4220, Fritz Götz (Tübingen, Germany) for S. carnosus TM300, Jörg Mostertz (Greifswald, Germany) for human cDNA, Jan Panné-Farré (Greifswald, Germany) for Listeria monocytogenes DNA and anti-ClpL antiserum, Christiane Wolz (Tübingen, Germany) for S. aureus SA113 Δ spa , and Olivier Tabary and Jacky Jacquot (Inserm, Paris, France) for upper-airway epithelial cells.

    Techniques: Immunofluorescence, Expressing