s. aureus Search Results


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  • 99
    ATCC s aureus
    TEM micrographs of S. aureus <t>ATCC</t> 29213 treated with the Negative control ( A ), Nano-BA PLGA ( B ), Nano-BA P85 with an incubation time of 30 min ( C ), 1 h ( D ), 2 h ( E ) and Polymyxin B ( F )
    S Aureus, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6722 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore s aureus
    TEM micrographs of S. aureus <t>ATCC</t> 29213 treated with the Negative control ( A ), Nano-BA PLGA ( B ), Nano-BA P85 with an incubation time of 30 min ( C ), 1 h ( D ), 2 h ( E ) and Polymyxin B ( F )
    S Aureus, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1360 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC s aureus atcc 25923
    TEM micrographs of S. aureus <t>ATCC</t> 29213 treated with the Negative control ( A ), Nano-BA PLGA ( B ), Nano-BA P85 with an incubation time of 30 min ( C ), 1 h ( D ), 2 h ( E ) and Polymyxin B ( F )
    S Aureus Atcc 25923, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC s aureus american type culture collection 6538
    TEM micrographs of S. aureus <t>ATCC</t> 29213 treated with the Negative control ( A ), Nano-BA PLGA ( B ), Nano-BA P85 with an incubation time of 30 min ( C ), 1 h ( D ), 2 h ( E ) and Polymyxin B ( F )
    S Aureus American Type Culture Collection 6538, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC s aureus atcc 43300
    Membrane effects. a SPR binding of vancomycin, 17 and 24 to L1 chips coated with lipid bilayers formed from DMPC or DMPG (errors are mean ± S.D, n = 3). b % haemolysis of human red blood cells in whole blood induced by a set of derivatives at 1600 µg mL −1 relative to complete lysis, compared with their calculated lipophilicity ( n = 2). c Membrane depolarisation: change in fluorescence intensity of the reporter dye diSC3(5) over 30 min following treatment of S. aureus <t>ATCC</t> 43300 (early exponential phase) with test compounds at 16 µg mL −1 (errors are mean ± S.D, n = 4). d Membrane permeabilisation: maximal fluorescence intensity of the reporter dye propidium iodide after 2 h following incubation of S. aureus ATCC 43300 (early exponential phase) with test compounds (16 µg mL −1 ) for 1 h at 37 °C (errors are mean ± S.D, n = 4)
    S Aureus Atcc 43300, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 761 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson s aureus
    Membrane effects. a SPR binding of vancomycin, 17 and 24 to L1 chips coated with lipid bilayers formed from DMPC or DMPG (errors are mean ± S.D, n = 3). b % haemolysis of human red blood cells in whole blood induced by a set of derivatives at 1600 µg mL −1 relative to complete lysis, compared with their calculated lipophilicity ( n = 2). c Membrane depolarisation: change in fluorescence intensity of the reporter dye diSC3(5) over 30 min following treatment of S. aureus <t>ATCC</t> 43300 (early exponential phase) with test compounds at 16 µg mL −1 (errors are mean ± S.D, n = 4). d Membrane permeabilisation: maximal fluorescence intensity of the reporter dye propidium iodide after 2 h following incubation of S. aureus ATCC 43300 (early exponential phase) with test compounds (16 µg mL −1 ) for 1 h at 37 °C (errors are mean ± S.D, n = 4)
    S Aureus, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 641 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC s aureus atcc
    Membrane effects. a SPR binding of vancomycin, 17 and 24 to L1 chips coated with lipid bilayers formed from DMPC or DMPG (errors are mean ± S.D, n = 3). b % haemolysis of human red blood cells in whole blood induced by a set of derivatives at 1600 µg mL −1 relative to complete lysis, compared with their calculated lipophilicity ( n = 2). c Membrane depolarisation: change in fluorescence intensity of the reporter dye diSC3(5) over 30 min following treatment of S. aureus <t>ATCC</t> 43300 (early exponential phase) with test compounds at 16 µg mL −1 (errors are mean ± S.D, n = 4). d Membrane permeabilisation: maximal fluorescence intensity of the reporter dye propidium iodide after 2 h following incubation of S. aureus ATCC 43300 (early exponential phase) with test compounds (16 µg mL −1 ) for 1 h at 37 °C (errors are mean ± S.D, n = 4)
    S Aureus Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 284 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Difco s aureus
    Membrane effects. a SPR binding of vancomycin, 17 and 24 to L1 chips coated with lipid bilayers formed from DMPC or DMPG (errors are mean ± S.D, n = 3). b % haemolysis of human red blood cells in whole blood induced by a set of derivatives at 1600 µg mL −1 relative to complete lysis, compared with their calculated lipophilicity ( n = 2). c Membrane depolarisation: change in fluorescence intensity of the reporter dye diSC3(5) over 30 min following treatment of S. aureus <t>ATCC</t> 43300 (early exponential phase) with test compounds at 16 µg mL −1 (errors are mean ± S.D, n = 4). d Membrane permeabilisation: maximal fluorescence intensity of the reporter dye propidium iodide after 2 h following incubation of S. aureus ATCC 43300 (early exponential phase) with test compounds (16 µg mL −1 ) for 1 h at 37 °C (errors are mean ± S.D, n = 4)
    S Aureus, supplied by Difco, used in various techniques. Bioz Stars score: 92/100, based on 401 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC s aureus atcc 12600
    (A) Micrographs of S. aureus <t>ATCC</t> 12600 adhering after 4 h in a parallel plate flow chamber. Scale bar is 40 μm. (B) Number of bacteria adhering after 4 h on glass, PEI-coated glass, microgel ( R h ≅ 100 nm each) coated glass with 1.5 mol % BIS, 5 mol % BIS, 15 mol % BIS cross-linking density. Statistically significant differences are indicated with **** ( p
    S Aureus Atcc 12600, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 432 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC s aureus atcc 6538p
    Effect of increasing concentrations of teicoplanin, NP-TEICO, IONPs, and NP-APTES on S. aureus <t>ATCC</t> <t>6538P</t> biofilm formation. In the case of NP preparations, the amounts to be added were defined considering the teicoplanin loaded on IONPs under the conditions defined in the Materials and Methods. Effect on adherent biomass following crystal violet staining (A) . Effect on planktonic (B) and adherent (C) cells exposed to teicoplanin (orange), IONPs (red), NP-APTES (green), and NP-TEICO (violet) on viability assay. The values are expressed as mean ± SD of three independent experiments. One-way ANOVA analyses, ∗ p
    S Aureus Atcc 6538p, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 277 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC s aureus atcc 33591
    Aspidinol is effective in a mouse model of MRSA septicemic infection. (A) Ten mice per group were infected (i.p.) with a lethal dose of S. aureus <t>ATCC</t> 33591 and intravenously injected with aspidinol, vancomycin (5, 15, or 25 mg/kg), or the vehicle alone for 5 days (one dose per day). Mice were monitored for 5 days and the percentage survival was calculated. The statistical significance was calculated in order to compare treated to control groups. (B) Six mice per group were infected (i.p.) with a non-lethal dose of S. aureus ATCC 33591 and intravenously injected with aspidinol, vancomycin (25 mg/kg) or the vehicle alone for 6 days (one dose per day). Twenty-four hours after the last treatment, the mice were euthanized, and their organs were excised and homogenized in TSB to count viable MRSA colonies. The number of CFUs from each mouse is plotted as individual points. Values are the mean of triplicate samples with standard deviation bars. (C) Histological evaluation of lung and liver of mice infected with S. aureus ATCC 33591 receiving no treatment or a treatment with aspidinol. Both lung and liver in the control group demonstrated acute inflammation; no apparent pathological changes were observed in the treatment group.
    S Aureus Atcc 33591, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 398 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher s aureus
    Aspidinol is effective in a mouse model of MRSA septicemic infection. (A) Ten mice per group were infected (i.p.) with a lethal dose of S. aureus <t>ATCC</t> 33591 and intravenously injected with aspidinol, vancomycin (5, 15, or 25 mg/kg), or the vehicle alone for 5 days (one dose per day). Mice were monitored for 5 days and the percentage survival was calculated. The statistical significance was calculated in order to compare treated to control groups. (B) Six mice per group were infected (i.p.) with a non-lethal dose of S. aureus ATCC 33591 and intravenously injected with aspidinol, vancomycin (25 mg/kg) or the vehicle alone for 6 days (one dose per day). Twenty-four hours after the last treatment, the mice were euthanized, and their organs were excised and homogenized in TSB to count viable MRSA colonies. The number of CFUs from each mouse is plotted as individual points. Values are the mean of triplicate samples with standard deviation bars. (C) Histological evaluation of lung and liver of mice infected with S. aureus ATCC 33591 receiving no treatment or a treatment with aspidinol. Both lung and liver in the control group demonstrated acute inflammation; no apparent pathological changes were observed in the treatment group.
    S Aureus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1887 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Clinical and Laboratory Standards Institute s aureus
    Aspidinol is effective in a mouse model of MRSA septicemic infection. (A) Ten mice per group were infected (i.p.) with a lethal dose of S. aureus <t>ATCC</t> 33591 and intravenously injected with aspidinol, vancomycin (5, 15, or 25 mg/kg), or the vehicle alone for 5 days (one dose per day). Mice were monitored for 5 days and the percentage survival was calculated. The statistical significance was calculated in order to compare treated to control groups. (B) Six mice per group were infected (i.p.) with a non-lethal dose of S. aureus ATCC 33591 and intravenously injected with aspidinol, vancomycin (25 mg/kg) or the vehicle alone for 6 days (one dose per day). Twenty-four hours after the last treatment, the mice were euthanized, and their organs were excised and homogenized in TSB to count viable MRSA colonies. The number of CFUs from each mouse is plotted as individual points. Values are the mean of triplicate samples with standard deviation bars. (C) Histological evaluation of lung and liver of mice infected with S. aureus ATCC 33591 receiving no treatment or a treatment with aspidinol. Both lung and liver in the control group demonstrated acute inflammation; no apparent pathological changes were observed in the treatment group.
    S Aureus, supplied by Clinical and Laboratory Standards Institute, used in various techniques. Bioz Stars score: 92/100, based on 229 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Bio-Rad s aureus
    Aspidinol is effective in a mouse model of MRSA septicemic infection. (A) Ten mice per group were infected (i.p.) with a lethal dose of S. aureus <t>ATCC</t> 33591 and intravenously injected with aspidinol, vancomycin (5, 15, or 25 mg/kg), or the vehicle alone for 5 days (one dose per day). Mice were monitored for 5 days and the percentage survival was calculated. The statistical significance was calculated in order to compare treated to control groups. (B) Six mice per group were infected (i.p.) with a non-lethal dose of S. aureus ATCC 33591 and intravenously injected with aspidinol, vancomycin (25 mg/kg) or the vehicle alone for 6 days (one dose per day). Twenty-four hours after the last treatment, the mice were euthanized, and their organs were excised and homogenized in TSB to count viable MRSA colonies. The number of CFUs from each mouse is plotted as individual points. Values are the mean of triplicate samples with standard deviation bars. (C) Histological evaluation of lung and liver of mice infected with S. aureus ATCC 33591 receiving no treatment or a treatment with aspidinol. Both lung and liver in the control group demonstrated acute inflammation; no apparent pathological changes were observed in the treatment group.
    S Aureus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 369 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC s aureus atcc 700699
    Metabolic activity and viability of biofilms after treatment with honey (mean and standard deviation). Metabolic activity was determined by TTC metabolism and viability was determined by viable counts. Metabolic activity of ( a ) Pseudomonas aeruginosa BAA-47 ( b ) Enterococcus faecalis NCTC 775 and ( c ) Staphylococcus aureus ATCC 700699, and viable counts of ( d ) Pseudomonas aeruginosa BAA-47 ( e ) Enterococcus faecalis NCTC 775 and ( f ) Staphylococcus aureus <t>ATCC</t> 700699 recovered from multispecies biofilms. Metabolic activity data were analysed by 2-way ANOVA and Tukey’s multiple comparisons post-hoc test. All honey-treated biofilms ( a – c ) differed significantly from the untreated control (significance not shown on graphs). Viable count data ( d , e , f ) was analysed by one-way ANOVA followed by Tukey’s post-hoc test and all honey-treated biofilms differed significantly from the untreated control. Asterisks indicate significant differences compared to the control ( d – f ), or between treatments ( b ) (* P
    S Aureus Atcc 700699, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore s aureus strains
    Metabolic activity and viability of biofilms after treatment with honey (mean and standard deviation). Metabolic activity was determined by TTC metabolism and viability was determined by viable counts. Metabolic activity of ( a ) Pseudomonas aeruginosa BAA-47 ( b ) Enterococcus faecalis NCTC 775 and ( c ) Staphylococcus aureus ATCC 700699, and viable counts of ( d ) Pseudomonas aeruginosa BAA-47 ( e ) Enterococcus faecalis NCTC 775 and ( f ) Staphylococcus aureus <t>ATCC</t> 700699 recovered from multispecies biofilms. Metabolic activity data were analysed by 2-way ANOVA and Tukey’s multiple comparisons post-hoc test. All honey-treated biofilms ( a – c ) differed significantly from the untreated control (significance not shown on graphs). Viable count data ( d , e , f ) was analysed by one-way ANOVA followed by Tukey’s post-hoc test and all honey-treated biofilms differed significantly from the untreated control. Asterisks indicate significant differences compared to the control ( d – f ), or between treatments ( b ) (* P
    S Aureus Strains, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    N/A
    Staphylococcus aureus Enterotoxin I SEI is a mouse monoclonal antibody to infectious agent toxin Isotype Note IgG1 Host Note Mouse Conjugation Note Unconjugated Application Note ELISA WB Purification
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    Rabbit Anti S Aureus S Aureus Delta Hemolysin Antibody 400 µl
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    Staphylococcus aureus Enterotoxin G SEG is a mouse monoclonal antibody to infectious agent toxin Isotype Note IgG1 Host Note Mouse Conjugation Note Unconjugated Application Note ELISA WB Purification
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    Mouse monoclonal Staphylococcus aureus antibody
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    Image Search Results


    TEM micrographs of S. aureus ATCC 29213 treated with the Negative control ( A ), Nano-BA PLGA ( B ), Nano-BA P85 with an incubation time of 30 min ( C ), 1 h ( D ), 2 h ( E ) and Polymyxin B ( F )

    Journal: Journal of Nanobiotechnology

    Article Title: Pluronic-based nano-self-assemblies of bacitracin A with a new mechanism of action for an efficient in vivo therapeutic effect against bacterial peritonitis

    doi: 10.1186/s12951-018-0397-3

    Figure Lengend Snippet: TEM micrographs of S. aureus ATCC 29213 treated with the Negative control ( A ), Nano-BA PLGA ( B ), Nano-BA P85 with an incubation time of 30 min ( C ), 1 h ( D ), 2 h ( E ) and Polymyxin B ( F )

    Article Snippet: Mice were randomly divided into 12 groups (n = 6) and intraperitoneally injected with 109 colony-forming units (CFU)/mL of E. coli ATCC 25922, S. aureus ATCC 29213 and a mixture of E. coli ATCC 25922 and S. aureus ATCC 29213 in 200 μL of sterile isotonic saline [ ].

    Techniques: Transmission Electron Microscopy, Negative Control, Incubation

    Release of intracellular K +  in the presence of paenipeptin C′ as detected using a potassium-sensitive fluorescent probe, PBFI.  a Pseudomonas aeruginosa  ATCC 27853 ( b )  Staphylococcus aureus  ATCC 29213. Means with different letters are significantly different between groups ( p

    Journal: BMC Microbiology

    Article Title: Novel linear lipopeptide paenipeptin C′ binds to lipopolysaccharides and lipoteichoic acid and exerts bactericidal activity by the disruption of cytoplasmic membrane

    doi: 10.1186/s12866-018-1381-7

    Figure Lengend Snippet: Release of intracellular K + in the presence of paenipeptin C′ as detected using a potassium-sensitive fluorescent probe, PBFI. a Pseudomonas aeruginosa ATCC 27853 ( b ) Staphylococcus aureus ATCC 29213. Means with different letters are significantly different between groups ( p

    Article Snippet: Similarly, significant potassium ion leakage from S. aureus ATCC 29213 cells was observed after treatment with paenipeptin C' at 64 μg/mL (Fig. b).

    Techniques:

    Examination of the cell morphology change after paenipeptin C′ treatment using transmission electron microscopy (TEM). a Pseudomonas aeruginosa ATCC 27853 cells were treated by paenipeptin C′ at 16 μg/mL for 2 h. b Non-treated P. aeruginosa ATCC 27853 cells. c Staphylococcus aureus ATCC 29213 cells were treated by paenipeptin C' at 32 μg/mL for 2 h. d Non-treated S. aureus ATCC 29213 cells

    Journal: BMC Microbiology

    Article Title: Novel linear lipopeptide paenipeptin C′ binds to lipopolysaccharides and lipoteichoic acid and exerts bactericidal activity by the disruption of cytoplasmic membrane

    doi: 10.1186/s12866-018-1381-7

    Figure Lengend Snippet: Examination of the cell morphology change after paenipeptin C′ treatment using transmission electron microscopy (TEM). a Pseudomonas aeruginosa ATCC 27853 cells were treated by paenipeptin C′ at 16 μg/mL for 2 h. b Non-treated P. aeruginosa ATCC 27853 cells. c Staphylococcus aureus ATCC 29213 cells were treated by paenipeptin C' at 32 μg/mL for 2 h. d Non-treated S. aureus ATCC 29213 cells

    Article Snippet: Similarly, significant potassium ion leakage from S. aureus ATCC 29213 cells was observed after treatment with paenipeptin C' at 64 μg/mL (Fig. b).

    Techniques: Transmission Assay, Electron Microscopy, Transmission Electron Microscopy

    Concentration-dependent bactericidal activity of lipopeptide paenipeptin C′ against ( a ) Pseudomonas aeruginosa ATCC 27853 and ( b ) Staphylococcus aureus ATCC 29213

    Journal: BMC Microbiology

    Article Title: Novel linear lipopeptide paenipeptin C′ binds to lipopolysaccharides and lipoteichoic acid and exerts bactericidal activity by the disruption of cytoplasmic membrane

    doi: 10.1186/s12866-018-1381-7

    Figure Lengend Snippet: Concentration-dependent bactericidal activity of lipopeptide paenipeptin C′ against ( a ) Pseudomonas aeruginosa ATCC 27853 and ( b ) Staphylococcus aureus ATCC 29213

    Article Snippet: Similarly, significant potassium ion leakage from S. aureus ATCC 29213 cells was observed after treatment with paenipeptin C' at 64 μg/mL (Fig. b).

    Techniques: Concentration Assay, Activity Assay

    Changes in bacterial membrane potential in the presence of paenipeptin C′ as determined using a membrane potential-sensitive fluorescent probe, DiSC 3 (5).  a Pseudomonas aeruginosa  ATCC 27853 ( b )  Staphylococcus aureus  ATCC 29213. Means with different letters are significantly different between groups ( p

    Journal: BMC Microbiology

    Article Title: Novel linear lipopeptide paenipeptin C′ binds to lipopolysaccharides and lipoteichoic acid and exerts bactericidal activity by the disruption of cytoplasmic membrane

    doi: 10.1186/s12866-018-1381-7

    Figure Lengend Snippet: Changes in bacterial membrane potential in the presence of paenipeptin C′ as determined using a membrane potential-sensitive fluorescent probe, DiSC 3 (5). a Pseudomonas aeruginosa ATCC 27853 ( b ) Staphylococcus aureus ATCC 29213. Means with different letters are significantly different between groups ( p

    Article Snippet: Similarly, significant potassium ion leakage from S. aureus ATCC 29213 cells was observed after treatment with paenipeptin C' at 64 μg/mL (Fig. b).

    Techniques:

    Impact of lipopolysaccharides (LPS) and lipoteichoic acid (LTA) on antimicrobial activity of paenipeptin C′ against ( a )  Pseudomonas aeruginosa  ATCC 27853 and ( b )  Staphylococcus aureus  ATCC 29213. The nontreated cells (Non) without antibiotics and LPS/LTA were used as control groups. Means with different letters are significantly different between groups ( p

    Journal: BMC Microbiology

    Article Title: Novel linear lipopeptide paenipeptin C′ binds to lipopolysaccharides and lipoteichoic acid and exerts bactericidal activity by the disruption of cytoplasmic membrane

    doi: 10.1186/s12866-018-1381-7

    Figure Lengend Snippet: Impact of lipopolysaccharides (LPS) and lipoteichoic acid (LTA) on antimicrobial activity of paenipeptin C′ against ( a ) Pseudomonas aeruginosa ATCC 27853 and ( b ) Staphylococcus aureus ATCC 29213. The nontreated cells (Non) without antibiotics and LPS/LTA were used as control groups. Means with different letters are significantly different between groups ( p

    Article Snippet: Similarly, significant potassium ion leakage from S. aureus ATCC 29213 cells was observed after treatment with paenipeptin C' at 64 μg/mL (Fig. b).

    Techniques: Activity Assay

    Membrane effects. a SPR binding of vancomycin, 17 and 24 to L1 chips coated with lipid bilayers formed from DMPC or DMPG (errors are mean ± S.D, n = 3). b % haemolysis of human red blood cells in whole blood induced by a set of derivatives at 1600 µg mL −1 relative to complete lysis, compared with their calculated lipophilicity ( n = 2). c Membrane depolarisation: change in fluorescence intensity of the reporter dye diSC3(5) over 30 min following treatment of S. aureus ATCC 43300 (early exponential phase) with test compounds at 16 µg mL −1 (errors are mean ± S.D, n = 4). d Membrane permeabilisation: maximal fluorescence intensity of the reporter dye propidium iodide after 2 h following incubation of S. aureus ATCC 43300 (early exponential phase) with test compounds (16 µg mL −1 ) for 1 h at 37 °C (errors are mean ± S.D, n = 4)

    Journal: Nature Communications

    Article Title: Protein-inspired antibiotics active against vancomycin- and daptomycin-resistant bacteria

    doi: 10.1038/s41467-017-02123-w

    Figure Lengend Snippet: Membrane effects. a SPR binding of vancomycin, 17 and 24 to L1 chips coated with lipid bilayers formed from DMPC or DMPG (errors are mean ± S.D, n = 3). b % haemolysis of human red blood cells in whole blood induced by a set of derivatives at 1600 µg mL −1 relative to complete lysis, compared with their calculated lipophilicity ( n = 2). c Membrane depolarisation: change in fluorescence intensity of the reporter dye diSC3(5) over 30 min following treatment of S. aureus ATCC 43300 (early exponential phase) with test compounds at 16 µg mL −1 (errors are mean ± S.D, n = 4). d Membrane permeabilisation: maximal fluorescence intensity of the reporter dye propidium iodide after 2 h following incubation of S. aureus ATCC 43300 (early exponential phase) with test compounds (16 µg mL −1 ) for 1 h at 37 °C (errors are mean ± S.D, n = 4)

    Article Snippet: S. aureus ATCC® 43300 ™ was grown in Mueller Hinton broth supplemented with 50 µg mL−1 of CaCl2 .

    Techniques: SPR Assay, Binding Assay, Lysis, Fluorescence, Incubation

    Time kill assay. Bactericidal activity of vancomycin 1 and vancapticin 24 against S. aureus ATCC 43300 as measured by agar plate dilution colony measurement of wells from broth microdilution MIC measurement over time. Data are n = 1

    Journal: Nature Communications

    Article Title: Protein-inspired antibiotics active against vancomycin- and daptomycin-resistant bacteria

    doi: 10.1038/s41467-017-02123-w

    Figure Lengend Snippet: Time kill assay. Bactericidal activity of vancomycin 1 and vancapticin 24 against S. aureus ATCC 43300 as measured by agar plate dilution colony measurement of wells from broth microdilution MIC measurement over time. Data are n = 1

    Article Snippet: S. aureus ATCC® 43300 ™ was grown in Mueller Hinton broth supplemented with 50 µg mL−1 of CaCl2 .

    Techniques: Time-Kill Assay, Activity Assay

    (A) Micrographs of S. aureus ATCC 12600 adhering after 4 h in a parallel plate flow chamber. Scale bar is 40 μm. (B) Number of bacteria adhering after 4 h on glass, PEI-coated glass, microgel ( R h ≅ 100 nm each) coated glass with 1.5 mol % BIS, 5 mol % BIS, 15 mol % BIS cross-linking density. Statistically significant differences are indicated with **** ( p

    Journal: Biomacromolecules

    Article Title: Inhibiting Bacterial Adhesion by Mechanically Modulated Microgel Coatings

    doi: 10.1021/acs.biomac.8b01378

    Figure Lengend Snippet: (A) Micrographs of S. aureus ATCC 12600 adhering after 4 h in a parallel plate flow chamber. Scale bar is 40 μm. (B) Number of bacteria adhering after 4 h on glass, PEI-coated glass, microgel ( R h ≅ 100 nm each) coated glass with 1.5 mol % BIS, 5 mol % BIS, 15 mol % BIS cross-linking density. Statistically significant differences are indicated with **** ( p

    Article Snippet: The results show that the initial adhesion rate of S. aureus ATCC 12600 is extremely reduced on the microgel coated surfaces.

    Techniques: Flow Cytometry

    Initial deposition rates ( j 0 ) of S. aureus ATCC 12600 on uncoated, PEI coated and microgel coated-glass surfaces. Statistically significant differences are indicated with **** ( p

    Journal: Biomacromolecules

    Article Title: Inhibiting Bacterial Adhesion by Mechanically Modulated Microgel Coatings

    doi: 10.1021/acs.biomac.8b01378

    Figure Lengend Snippet: Initial deposition rates ( j 0 ) of S. aureus ATCC 12600 on uncoated, PEI coated and microgel coated-glass surfaces. Statistically significant differences are indicated with **** ( p

    Article Snippet: The results show that the initial adhesion rate of S. aureus ATCC 12600 is extremely reduced on the microgel coated surfaces.

    Techniques:

    (A) Micrographs of S. aureus ATCC 12600 adhering after 4 h in a parallel plate flow chamber. Scale bar is 40 μm. (B) Number of bacteria adhering after 4 h on glass, PEI-coated glass, microgel coated glass with a cross-linking density of 1.5 mol % BIS ( R h = 650 nm), 5 mol % BIS ( R h = 301 nm) and 15 mol % BIS ( R h =101 nm), and a thickness h = 54−59 nm. Statistically significant differences are indicated with * ( p

    Journal: Biomacromolecules

    Article Title: Inhibiting Bacterial Adhesion by Mechanically Modulated Microgel Coatings

    doi: 10.1021/acs.biomac.8b01378

    Figure Lengend Snippet: (A) Micrographs of S. aureus ATCC 12600 adhering after 4 h in a parallel plate flow chamber. Scale bar is 40 μm. (B) Number of bacteria adhering after 4 h on glass, PEI-coated glass, microgel coated glass with a cross-linking density of 1.5 mol % BIS ( R h = 650 nm), 5 mol % BIS ( R h = 301 nm) and 15 mol % BIS ( R h =101 nm), and a thickness h = 54−59 nm. Statistically significant differences are indicated with * ( p

    Article Snippet: The results show that the initial adhesion rate of S. aureus ATCC 12600 is extremely reduced on the microgel coated surfaces.

    Techniques: Flow Cytometry

    ((A) Micrographs of S. aureus ATCC 12600 adhering after 4 h in a parallel plate flow chamber. Scale bar is 40 μm. (B) Number of bacteria adhering after 4 h on glass, PEI-coated glass, microgel coated glass with the same cross-linking density (1.5 mol % BIS) but with different coating thicknesses as h = 10, 54 and 602 nm. Statistically significant differences are indicated with ** ( p

    Journal: Biomacromolecules

    Article Title: Inhibiting Bacterial Adhesion by Mechanically Modulated Microgel Coatings

    doi: 10.1021/acs.biomac.8b01378

    Figure Lengend Snippet: ((A) Micrographs of S. aureus ATCC 12600 adhering after 4 h in a parallel plate flow chamber. Scale bar is 40 μm. (B) Number of bacteria adhering after 4 h on glass, PEI-coated glass, microgel coated glass with the same cross-linking density (1.5 mol % BIS) but with different coating thicknesses as h = 10, 54 and 602 nm. Statistically significant differences are indicated with ** ( p

    Article Snippet: The results show that the initial adhesion rate of S. aureus ATCC 12600 is extremely reduced on the microgel coated surfaces.

    Techniques: Flow Cytometry

    Effect of increasing concentrations of teicoplanin, NP-TEICO, IONPs, and NP-APTES on S. aureus ATCC 6538P biofilm formation. In the case of NP preparations, the amounts to be added were defined considering the teicoplanin loaded on IONPs under the conditions defined in the Materials and Methods. Effect on adherent biomass following crystal violet staining (A) . Effect on planktonic (B) and adherent (C) cells exposed to teicoplanin (orange), IONPs (red), NP-APTES (green), and NP-TEICO (violet) on viability assay. The values are expressed as mean ± SD of three independent experiments. One-way ANOVA analyses, ∗ p

    Journal: Frontiers in Microbiology

    Article Title: Magnetic Nanoconjugated Teicoplanin: A Novel Tool for Bacterial Infection Site Targeting

    doi: 10.3389/fmicb.2018.02270

    Figure Lengend Snippet: Effect of increasing concentrations of teicoplanin, NP-TEICO, IONPs, and NP-APTES on S. aureus ATCC 6538P biofilm formation. In the case of NP preparations, the amounts to be added were defined considering the teicoplanin loaded on IONPs under the conditions defined in the Materials and Methods. Effect on adherent biomass following crystal violet staining (A) . Effect on planktonic (B) and adherent (C) cells exposed to teicoplanin (orange), IONPs (red), NP-APTES (green), and NP-TEICO (violet) on viability assay. The values are expressed as mean ± SD of three independent experiments. One-way ANOVA analyses, ∗ p

    Article Snippet: Consistently, the antimicrobial activity of NP-TEICO – measured by the antimicrobial susceptibility test versus S. aureus ATCC 6538P and B. subtilis ATCC 6633 (see below) – was also maintained.

    Techniques: Staining, Viability Assay

    TEM images of S. aureus ATCC 6538P (A–C) exposed to NP-APTES (A) , NP-TEICO (B) , teicoplanin (C) , and B. subtilis ATCC 6633 (D,E) exposed to NP-TEICO (D) and teicoplanin (E) . Scale bar: 500 nm. indicates ghost cells; ∗ indicates lysed cells; white arrows indicate mesosome-like structures.

    Journal: Frontiers in Microbiology

    Article Title: Magnetic Nanoconjugated Teicoplanin: A Novel Tool for Bacterial Infection Site Targeting

    doi: 10.3389/fmicb.2018.02270

    Figure Lengend Snippet: TEM images of S. aureus ATCC 6538P (A–C) exposed to NP-APTES (A) , NP-TEICO (B) , teicoplanin (C) , and B. subtilis ATCC 6633 (D,E) exposed to NP-TEICO (D) and teicoplanin (E) . Scale bar: 500 nm. indicates ghost cells; ∗ indicates lysed cells; white arrows indicate mesosome-like structures.

    Article Snippet: Consistently, the antimicrobial activity of NP-TEICO – measured by the antimicrobial susceptibility test versus S. aureus ATCC 6538P and B. subtilis ATCC 6633 (see below) – was also maintained.

    Techniques: Transmission Electron Microscopy

    Population growth kinetics of S. aureus ATCC 6538P (A) , B. subtilis ATCC 6633 (B) , and E. coli ATCC 35218 (C) exposed to teicoplanin (orange), IONPs (red), NP-APTES (green), and NP-TEICO (violet). Cultures without any addition (blue) were used as controls. Growth was recorded for 5 h. Black arrows indicate the addition (after 1 h of growth) of NP preparations and of teicoplanin to the bacterial populations. Triplicate experiments were conducted for each condition: standard errors were lower than 5%.

    Journal: Frontiers in Microbiology

    Article Title: Magnetic Nanoconjugated Teicoplanin: A Novel Tool for Bacterial Infection Site Targeting

    doi: 10.3389/fmicb.2018.02270

    Figure Lengend Snippet: Population growth kinetics of S. aureus ATCC 6538P (A) , B. subtilis ATCC 6633 (B) , and E. coli ATCC 35218 (C) exposed to teicoplanin (orange), IONPs (red), NP-APTES (green), and NP-TEICO (violet). Cultures without any addition (blue) were used as controls. Growth was recorded for 5 h. Black arrows indicate the addition (after 1 h of growth) of NP preparations and of teicoplanin to the bacterial populations. Triplicate experiments were conducted for each condition: standard errors were lower than 5%.

    Article Snippet: Consistently, the antimicrobial activity of NP-TEICO – measured by the antimicrobial susceptibility test versus S. aureus ATCC 6538P and B. subtilis ATCC 6633 (see below) – was also maintained.

    Techniques:

    Fluorescence microscopy images of live and dead cells of S. aureus ATCC 6538P [first column on the left: (A,D,G,J,M) ], B. subtilis ATCC 6633 [middle column: (B,E,H,K,N) ], and E. coli ATCC 35218 [column on the right: (C,F,I,L,O) ] in the absence and presence of different NP preparations and of teicoplanin. (A–C) untreated cells; (D–F) cells treated with IONPs; (G–I) cells treated with NP-APTES; (J–L) cells treated with NP-TEICO; (M–O) cells treated with teicoplanin. Scale bar: 12 μm.

    Journal: Frontiers in Microbiology

    Article Title: Magnetic Nanoconjugated Teicoplanin: A Novel Tool for Bacterial Infection Site Targeting

    doi: 10.3389/fmicb.2018.02270

    Figure Lengend Snippet: Fluorescence microscopy images of live and dead cells of S. aureus ATCC 6538P [first column on the left: (A,D,G,J,M) ], B. subtilis ATCC 6633 [middle column: (B,E,H,K,N) ], and E. coli ATCC 35218 [column on the right: (C,F,I,L,O) ] in the absence and presence of different NP preparations and of teicoplanin. (A–C) untreated cells; (D–F) cells treated with IONPs; (G–I) cells treated with NP-APTES; (J–L) cells treated with NP-TEICO; (M–O) cells treated with teicoplanin. Scale bar: 12 μm.

    Article Snippet: Consistently, the antimicrobial activity of NP-TEICO – measured by the antimicrobial susceptibility test versus S. aureus ATCC 6538P and B. subtilis ATCC 6633 (see below) – was also maintained.

    Techniques: Fluorescence, Microscopy

    TEM images of S. aureus ATCC 6538P [first column on the left: (A,D,G,J,M) ], B. subtilis ATCC 6633 [middle column: (B,E,H,K,N) ], and E. coli ATCC 35218 [column on the right: (C,F,I,L,O) ] cells in the absence and presence of different NP preparations and of teicoplanin. (A–C) untreated cells; (D–F) cells treated with IONPs; (G–I) cells exposed to NP-APTES; (J–L) cells exposed to NP-TEICO; (M–O) cells treated with teicoplanin. Scale bars: 1 μm.

    Journal: Frontiers in Microbiology

    Article Title: Magnetic Nanoconjugated Teicoplanin: A Novel Tool for Bacterial Infection Site Targeting

    doi: 10.3389/fmicb.2018.02270

    Figure Lengend Snippet: TEM images of S. aureus ATCC 6538P [first column on the left: (A,D,G,J,M) ], B. subtilis ATCC 6633 [middle column: (B,E,H,K,N) ], and E. coli ATCC 35218 [column on the right: (C,F,I,L,O) ] cells in the absence and presence of different NP preparations and of teicoplanin. (A–C) untreated cells; (D–F) cells treated with IONPs; (G–I) cells exposed to NP-APTES; (J–L) cells exposed to NP-TEICO; (M–O) cells treated with teicoplanin. Scale bars: 1 μm.

    Article Snippet: Consistently, the antimicrobial activity of NP-TEICO – measured by the antimicrobial susceptibility test versus S. aureus ATCC 6538P and B. subtilis ATCC 6633 (see below) – was also maintained.

    Techniques: Transmission Electron Microscopy

    Aspidinol is effective in a mouse model of MRSA septicemic infection. (A) Ten mice per group were infected (i.p.) with a lethal dose of S. aureus ATCC 33591 and intravenously injected with aspidinol, vancomycin (5, 15, or 25 mg/kg), or the vehicle alone for 5 days (one dose per day). Mice were monitored for 5 days and the percentage survival was calculated. The statistical significance was calculated in order to compare treated to control groups. (B) Six mice per group were infected (i.p.) with a non-lethal dose of S. aureus ATCC 33591 and intravenously injected with aspidinol, vancomycin (25 mg/kg) or the vehicle alone for 6 days (one dose per day). Twenty-four hours after the last treatment, the mice were euthanized, and their organs were excised and homogenized in TSB to count viable MRSA colonies. The number of CFUs from each mouse is plotted as individual points. Values are the mean of triplicate samples with standard deviation bars. (C) Histological evaluation of lung and liver of mice infected with S. aureus ATCC 33591 receiving no treatment or a treatment with aspidinol. Both lung and liver in the control group demonstrated acute inflammation; no apparent pathological changes were observed in the treatment group.

    Journal: Frontiers in Pharmacology

    Article Title: Antibacterial Activity and Mechanism of Action of Aspidinol Against Multi-Drug-Resistant Methicillin-Resistant Staphylococcus aureus

    doi: 10.3389/fphar.2018.00619

    Figure Lengend Snippet: Aspidinol is effective in a mouse model of MRSA septicemic infection. (A) Ten mice per group were infected (i.p.) with a lethal dose of S. aureus ATCC 33591 and intravenously injected with aspidinol, vancomycin (5, 15, or 25 mg/kg), or the vehicle alone for 5 days (one dose per day). Mice were monitored for 5 days and the percentage survival was calculated. The statistical significance was calculated in order to compare treated to control groups. (B) Six mice per group were infected (i.p.) with a non-lethal dose of S. aureus ATCC 33591 and intravenously injected with aspidinol, vancomycin (25 mg/kg) or the vehicle alone for 6 days (one dose per day). Twenty-four hours after the last treatment, the mice were euthanized, and their organs were excised and homogenized in TSB to count viable MRSA colonies. The number of CFUs from each mouse is plotted as individual points. Values are the mean of triplicate samples with standard deviation bars. (C) Histological evaluation of lung and liver of mice infected with S. aureus ATCC 33591 receiving no treatment or a treatment with aspidinol. Both lung and liver in the control group demonstrated acute inflammation; no apparent pathological changes were observed in the treatment group.

    Article Snippet: Compared to linezolid and vancomycin, aspidinol was not able to significantly reduce adherent biofilms of S. aureus ATCC 33591.

    Techniques: Infection, Mouse Assay, Injection, Standard Deviation

    Anti-biofilm activity of aspidinol. (A) The effects of aspidinol and antibiotics (linezolid and vancomycin) on established biofilms of S. aureus ATCC 33591 were evaluated. The pre-formed biofilms were treated with linezolid, vancomycin, or aspidinol and then stained with crystal violet. The optical density of the dissolved crystal violet was measured using a spectrophotometer. The values are the mean of the triplicate samples with standard deviation bars. The results are given as the mean ± SD ( n = 3). Two-tailed Student’s t -test was employed, and ∗∗∗ means p -values ≤ 0.005. (B) Scanning electron microscopy images showing the structure of MRSA biofilms treated with aspidinol and antibiotics at 24 h. Magnifications, ×2000.

    Journal: Frontiers in Pharmacology

    Article Title: Antibacterial Activity and Mechanism of Action of Aspidinol Against Multi-Drug-Resistant Methicillin-Resistant Staphylococcus aureus

    doi: 10.3389/fphar.2018.00619

    Figure Lengend Snippet: Anti-biofilm activity of aspidinol. (A) The effects of aspidinol and antibiotics (linezolid and vancomycin) on established biofilms of S. aureus ATCC 33591 were evaluated. The pre-formed biofilms were treated with linezolid, vancomycin, or aspidinol and then stained with crystal violet. The optical density of the dissolved crystal violet was measured using a spectrophotometer. The values are the mean of the triplicate samples with standard deviation bars. The results are given as the mean ± SD ( n = 3). Two-tailed Student’s t -test was employed, and ∗∗∗ means p -values ≤ 0.005. (B) Scanning electron microscopy images showing the structure of MRSA biofilms treated with aspidinol and antibiotics at 24 h. Magnifications, ×2000.

    Article Snippet: Compared to linezolid and vancomycin, aspidinol was not able to significantly reduce adherent biofilms of S. aureus ATCC 33591.

    Techniques: Activity Assay, Staining, Spectrophotometry, Standard Deviation, Two Tailed Test, Electron Microscopy

    RNA-Seq gene expression results for S. aureus ATCC 33591 cells treated and not treated with aspidinol. (A) A heatmap generated comparing aspidinol-treated cells to untreated control S. aureus ATCC 33591 cells is shown. Triplicate samples were used for each group. (B) Volcano plot analyses of unigenes in aspidinol and the ATCC 33591 group. (C) Differentially expressed genes enriched in the KEGG pathway.

    Journal: Frontiers in Pharmacology

    Article Title: Antibacterial Activity and Mechanism of Action of Aspidinol Against Multi-Drug-Resistant Methicillin-Resistant Staphylococcus aureus

    doi: 10.3389/fphar.2018.00619

    Figure Lengend Snippet: RNA-Seq gene expression results for S. aureus ATCC 33591 cells treated and not treated with aspidinol. (A) A heatmap generated comparing aspidinol-treated cells to untreated control S. aureus ATCC 33591 cells is shown. Triplicate samples were used for each group. (B) Volcano plot analyses of unigenes in aspidinol and the ATCC 33591 group. (C) Differentially expressed genes enriched in the KEGG pathway.

    Article Snippet: Compared to linezolid and vancomycin, aspidinol was not able to significantly reduce adherent biofilms of S. aureus ATCC 33591.

    Techniques: RNA Sequencing Assay, Expressing, Generated

    Time-kill analysis and the intracellular bacterial killing activity of aspidinol.  (A)  Time-kill kinetics of aspidinol against  S. aureus  ATCC 33591. The error bars show the standard deviations of the results from three independent biological repeats.  (B) S. aureus  ATCC 33591-infected RAW264.7 cells were treated with aspidinol and control antibiotics (vancomycin or linezolid) for 24 h, and the percent bacterial reduction was calculated compared to that of the untreated control groups. The results are given as the mean ± SD ( n  = 3). Two-tailed Student’s  t -test was employed, and  ∗ p -values of ≤0.05 are considered to be significant.

    Journal: Frontiers in Pharmacology

    Article Title: Antibacterial Activity and Mechanism of Action of Aspidinol Against Multi-Drug-Resistant Methicillin-Resistant Staphylococcus aureus

    doi: 10.3389/fphar.2018.00619

    Figure Lengend Snippet: Time-kill analysis and the intracellular bacterial killing activity of aspidinol. (A) Time-kill kinetics of aspidinol against S. aureus ATCC 33591. The error bars show the standard deviations of the results from three independent biological repeats. (B) S. aureus ATCC 33591-infected RAW264.7 cells were treated with aspidinol and control antibiotics (vancomycin or linezolid) for 24 h, and the percent bacterial reduction was calculated compared to that of the untreated control groups. The results are given as the mean ± SD ( n = 3). Two-tailed Student’s t -test was employed, and ∗ p -values of ≤0.05 are considered to be significant.

    Article Snippet: Compared to linezolid and vancomycin, aspidinol was not able to significantly reduce adherent biofilms of S. aureus ATCC 33591.

    Techniques: Activity Assay, Infection, Two Tailed Test

    Metabolic activity and viability of biofilms after treatment with honey (mean and standard deviation). Metabolic activity was determined by TTC metabolism and viability was determined by viable counts. Metabolic activity of ( a ) Pseudomonas aeruginosa BAA-47 ( b ) Enterococcus faecalis NCTC 775 and ( c ) Staphylococcus aureus ATCC 700699, and viable counts of ( d ) Pseudomonas aeruginosa BAA-47 ( e ) Enterococcus faecalis NCTC 775 and ( f ) Staphylococcus aureus ATCC 700699 recovered from multispecies biofilms. Metabolic activity data were analysed by 2-way ANOVA and Tukey’s multiple comparisons post-hoc test. All honey-treated biofilms ( a – c ) differed significantly from the untreated control (significance not shown on graphs). Viable count data ( d , e , f ) was analysed by one-way ANOVA followed by Tukey’s post-hoc test and all honey-treated biofilms differed significantly from the untreated control. Asterisks indicate significant differences compared to the control ( d – f ), or between treatments ( b ) (* P

    Journal: Scientific Reports

    Article Title: Anti-biofilm effects and characterisation of the hydrogen peroxide activity of a range of Western Australian honeys compared to Manuka and multifloral honeys

    doi: 10.1038/s41598-019-54217-8

    Figure Lengend Snippet: Metabolic activity and viability of biofilms after treatment with honey (mean and standard deviation). Metabolic activity was determined by TTC metabolism and viability was determined by viable counts. Metabolic activity of ( a ) Pseudomonas aeruginosa BAA-47 ( b ) Enterococcus faecalis NCTC 775 and ( c ) Staphylococcus aureus ATCC 700699, and viable counts of ( d ) Pseudomonas aeruginosa BAA-47 ( e ) Enterococcus faecalis NCTC 775 and ( f ) Staphylococcus aureus ATCC 700699 recovered from multispecies biofilms. Metabolic activity data were analysed by 2-way ANOVA and Tukey’s multiple comparisons post-hoc test. All honey-treated biofilms ( a – c ) differed significantly from the untreated control (significance not shown on graphs). Viable count data ( d , e , f ) was analysed by one-way ANOVA followed by Tukey’s post-hoc test and all honey-treated biofilms differed significantly from the untreated control. Asterisks indicate significant differences compared to the control ( d – f ), or between treatments ( b ) (* P

    Article Snippet: MICs of Jarrah 2 with and without the accumulation of hydrogen peroxide differed significantly (P = 0.025) against S. aureus ATCC 700699 only, whereas for Marri 2 MICs differed significantly for both S. aureus ATCC 700699 (P = 0.012) and E. faecalis NCTC 775 (P = 0.026).

    Techniques: Activity Assay, Standard Deviation

    Bacterial growth after 24 h in the presence of several concentrations of honey, determined by optical density at 600 nm (mean and standard deviation). ( a )  Pseudomonas aeruginosa  ATCC BAA-47 ( b )  Enterococcus faecalis  NCTC 775 and  (c) Staphylococcus aureus  ATCC 700699. Data were analysed by 2-way ANOVA and Tukey’s multiple comparisons post-hoc test. Asterisks indicate significant differences compared to the untreated control (* P

    Journal: Scientific Reports

    Article Title: Anti-biofilm effects and characterisation of the hydrogen peroxide activity of a range of Western Australian honeys compared to Manuka and multifloral honeys

    doi: 10.1038/s41598-019-54217-8

    Figure Lengend Snippet: Bacterial growth after 24 h in the presence of several concentrations of honey, determined by optical density at 600 nm (mean and standard deviation). ( a ) Pseudomonas aeruginosa ATCC BAA-47 ( b ) Enterococcus faecalis NCTC 775 and (c) Staphylococcus aureus ATCC 700699. Data were analysed by 2-way ANOVA and Tukey’s multiple comparisons post-hoc test. Asterisks indicate significant differences compared to the untreated control (* P

    Article Snippet: MICs of Jarrah 2 with and without the accumulation of hydrogen peroxide differed significantly (P = 0.025) against S. aureus ATCC 700699 only, whereas for Marri 2 MICs differed significantly for both S. aureus ATCC 700699 (P = 0.012) and E. faecalis NCTC 775 (P = 0.026).

    Techniques: Standard Deviation

    Biofilm formation after 24 h in the presence of several concentrations of honey, determined by crystal violet staining (mean and standard deviation). ( a )  Pseudomonas aeruginosa  ATCC BAA-47 ( b )  Enterococcus faecalis  NCTC 775 and ( c )  Staphylococcus aureus  ATCC 700699. Data were analysed by 2-way ANOVA and Tukey’s multiple comparisons post-hoc test. Asterisks indicate significant differences compared to the control (* P

    Journal: Scientific Reports

    Article Title: Anti-biofilm effects and characterisation of the hydrogen peroxide activity of a range of Western Australian honeys compared to Manuka and multifloral honeys

    doi: 10.1038/s41598-019-54217-8

    Figure Lengend Snippet: Biofilm formation after 24 h in the presence of several concentrations of honey, determined by crystal violet staining (mean and standard deviation). ( a ) Pseudomonas aeruginosa ATCC BAA-47 ( b ) Enterococcus faecalis NCTC 775 and ( c ) Staphylococcus aureus ATCC 700699. Data were analysed by 2-way ANOVA and Tukey’s multiple comparisons post-hoc test. Asterisks indicate significant differences compared to the control (* P

    Article Snippet: MICs of Jarrah 2 with and without the accumulation of hydrogen peroxide differed significantly (P = 0.025) against S. aureus ATCC 700699 only, whereas for Marri 2 MICs differed significantly for both S. aureus ATCC 700699 (P = 0.012) and E. faecalis NCTC 775 (P = 0.026).

    Techniques: Staining, Standard Deviation