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  • 94
    Vector Laboratories normal horse serum blocking solution
    Normal Horse Serum Blocking Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal horse serum blocking solution/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    normal horse serum blocking solution - by Bioz Stars, 2024-02
    94/100 stars
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    94
    Vector Laboratories blocking solution
    Blocking Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/blocking solution/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    blocking solution - by Bioz Stars, 2024-02
    94/100 stars
      Buy from Supplier

    94
    Vector Laboratories horse serum blocking solution
    Horse Serum Blocking Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horse serum blocking solution/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horse serum blocking solution - by Bioz Stars, 2024-02
    94/100 stars
      Buy from Supplier

    93
    Echelon Biosciences sphingosine 1 phosphate
    Specific detection of PtdIns(3,4,5) P 3 in the nucleolus. A , PIP strip (Echelon Inc) schematic showing the position of the spotted lipids each with 100 pmol. B , validation of the anti-PtdIns(3,4,5) P 3 antibody specificity using PIP strips. C , validation of the specificity of the recombinant GST-EGFP-GRP1-PH WT versus binding mutant K273A. D and E , confocal microscopy of actively growing HeLa cells stained with the indicated antibodies ( D ) or by incubation with recombinant GST-EGFP-GRP1-PH WT or K273A mutant combined with anti-nucleophosmin staining ( E ). F , quantification of the detection of nucleolar PtdIns(3,4,5) P 3 expressed as the percentage of HeLa cells showing foci detected by the GST-EGFP-GRP1-PH probe WT or K273A mutant within the area delimited by nucleophosmin (mean + SDs, n = 3, ∗ p < 0.05 two-way unpaired Student’s t test). The scale bar represents 5 μm. GRP1, general receptor for phosphoinositides-1; LPA, lysophosphatidic acid; LPC, lysophosphatidylcholine; PA, phosphatidic acid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PH, pleckstrin homology; PI, phosphatidylinositol; PIP3, PtdIns(3,4,5) P 3 ; PS, phosphatidylserine; PtdIns(3,4,5) P 3 , phosphatidylinositol 3,4,5-trisphosphate; <t>S1P,</t> <t>sphingosine-1-phosphate;</t> UBF, upstream binding factor.
    Sphingosine 1 Phosphate, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sphingosine 1 phosphate/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sphingosine 1 phosphate - by Bioz Stars, 2024-02
    93/100 stars
      Buy from Supplier

    94
    Vector Laboratories horse serum nhs
    Specific detection of PtdIns(3,4,5) P 3 in the nucleolus. A , PIP strip (Echelon Inc) schematic showing the position of the spotted lipids each with 100 pmol. B , validation of the anti-PtdIns(3,4,5) P 3 antibody specificity using PIP strips. C , validation of the specificity of the recombinant GST-EGFP-GRP1-PH WT versus binding mutant K273A. D and E , confocal microscopy of actively growing HeLa cells stained with the indicated antibodies ( D ) or by incubation with recombinant GST-EGFP-GRP1-PH WT or K273A mutant combined with anti-nucleophosmin staining ( E ). F , quantification of the detection of nucleolar PtdIns(3,4,5) P 3 expressed as the percentage of HeLa cells showing foci detected by the GST-EGFP-GRP1-PH probe WT or K273A mutant within the area delimited by nucleophosmin (mean + SDs, n = 3, ∗ p < 0.05 two-way unpaired Student’s t test). The scale bar represents 5 μm. GRP1, general receptor for phosphoinositides-1; LPA, lysophosphatidic acid; LPC, lysophosphatidylcholine; PA, phosphatidic acid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PH, pleckstrin homology; PI, phosphatidylinositol; PIP3, PtdIns(3,4,5) P 3 ; PS, phosphatidylserine; PtdIns(3,4,5) P 3 , phosphatidylinositol 3,4,5-trisphosphate; <t>S1P,</t> <t>sphingosine-1-phosphate;</t> UBF, upstream binding factor.
    Horse Serum Nhs, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horse serum nhs/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horse serum nhs - by Bioz Stars, 2024-02
    94/100 stars
      Buy from Supplier

    Image Search Results


    Specific detection of PtdIns(3,4,5) P 3 in the nucleolus. A , PIP strip (Echelon Inc) schematic showing the position of the spotted lipids each with 100 pmol. B , validation of the anti-PtdIns(3,4,5) P 3 antibody specificity using PIP strips. C , validation of the specificity of the recombinant GST-EGFP-GRP1-PH WT versus binding mutant K273A. D and E , confocal microscopy of actively growing HeLa cells stained with the indicated antibodies ( D ) or by incubation with recombinant GST-EGFP-GRP1-PH WT or K273A mutant combined with anti-nucleophosmin staining ( E ). F , quantification of the detection of nucleolar PtdIns(3,4,5) P 3 expressed as the percentage of HeLa cells showing foci detected by the GST-EGFP-GRP1-PH probe WT or K273A mutant within the area delimited by nucleophosmin (mean + SDs, n = 3, ∗ p < 0.05 two-way unpaired Student’s t test). The scale bar represents 5 μm. GRP1, general receptor for phosphoinositides-1; LPA, lysophosphatidic acid; LPC, lysophosphatidylcholine; PA, phosphatidic acid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PH, pleckstrin homology; PI, phosphatidylinositol; PIP3, PtdIns(3,4,5) P 3 ; PS, phosphatidylserine; PtdIns(3,4,5) P 3 , phosphatidylinositol 3,4,5-trisphosphate; S1P, sphingosine-1-phosphate; UBF, upstream binding factor.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Nuclear Phosphatidylinositol 3,4,5-Trisphosphate Interactome Uncovers an Enrichment in Nucleolar Proteins

    doi: 10.1016/j.mcpro.2021.100102

    Figure Lengend Snippet: Specific detection of PtdIns(3,4,5) P 3 in the nucleolus. A , PIP strip (Echelon Inc) schematic showing the position of the spotted lipids each with 100 pmol. B , validation of the anti-PtdIns(3,4,5) P 3 antibody specificity using PIP strips. C , validation of the specificity of the recombinant GST-EGFP-GRP1-PH WT versus binding mutant K273A. D and E , confocal microscopy of actively growing HeLa cells stained with the indicated antibodies ( D ) or by incubation with recombinant GST-EGFP-GRP1-PH WT or K273A mutant combined with anti-nucleophosmin staining ( E ). F , quantification of the detection of nucleolar PtdIns(3,4,5) P 3 expressed as the percentage of HeLa cells showing foci detected by the GST-EGFP-GRP1-PH probe WT or K273A mutant within the area delimited by nucleophosmin (mean + SDs, n = 3, ∗ p < 0.05 two-way unpaired Student’s t test). The scale bar represents 5 μm. GRP1, general receptor for phosphoinositides-1; LPA, lysophosphatidic acid; LPC, lysophosphatidylcholine; PA, phosphatidic acid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PH, pleckstrin homology; PI, phosphatidylinositol; PIP3, PtdIns(3,4,5) P 3 ; PS, phosphatidylserine; PtdIns(3,4,5) P 3 , phosphatidylinositol 3,4,5-trisphosphate; S1P, sphingosine-1-phosphate; UBF, upstream binding factor.

    Article Snippet: B , schematic representation of lipids spotted (100 pmol) on PIP strips (Echelon Biosciences) including lysophosphatidic acid (LPA), lysophosphatidylcholine (LPC), phosphatidylinositol (PtdIns), PtdIns3 P , PtdIns4 P , PtdIns5 P , phosphatidylethanolamine (PE), phosphatidylcholine (PC), sphingosine-1-Phosphate (S1P), PtdIns(3,4) P 2 , PtdIns(3,5) P 2 , PtdIns(4,5) P 2 , PtdIns(3,4,5) P 3 , phosphatic acid (PA), phosphatidylserine (PS), and blank.

    Techniques: Stripping Membranes, Recombinant, Binding Assay, Mutagenesis, Confocal Microscopy, Staining, Incubation

    PARP1 binds to PPIn via three polybasic regions. A , domain structure of PARP1 and deletion constructs. B , schematic representation of lipids spotted (100 pmol) on PIP strips (Echelon Biosciences) including lysophosphatidic acid (LPA), lysophosphatidylcholine (LPC), phosphatidylinositol (PtdIns), PtdIns3 P , PtdIns4 P , PtdIns5 P , phosphatidylethanolamine (PE), phosphatidylcholine (PC), sphingosine-1-Phosphate (S1P), PtdIns(3,4) P 2 , PtdIns(3,5) P 2 , PtdIns(4,5) P 2 , PtdIns(3,4,5) P 3 , phosphatic acid (PA), phosphatidylserine (PS), and blank. C and D , lipid overlay assay using PIP strips incubated with recombinant GST-PARP1 deletion constructs WT and mutants and detection of protein–lipid interactions using an anti-GST-HRP–conjugated antibody. E , multiple sequence alignment of the polybasic regions located in the zinc finger III and the BRCT-WGR linker found in human PARP-1 compared with other vertebrate species performed using the online program MUSCLE. Accession number for Homo sapiens (P09874), Mus musculus (P11103), Bos taurus (P18493), Gallus gallus (P26446), Xenopus laevis (P31669), and Danio rerio (Q5RHR0). F , ribbon representation of the human PARP-1 zinc-finger III NMR structure (aa 233–357 PDB: 2JVN, ). The two polybasic regions found in the N-terminal (aa 233–236) and C-terminal (aa 346–352) parts of the zinc-finger III are colored in red . ART, (ADP-ribosyl) transferase domain (ART); BRCT, BRCA1 C-terminal domain; HD, helical subdomain; HRP, horse radish peroxidase; PARP1, poly(ADP-ribose) polymerase 1; ZnF 1 to 3, zinc-finger I-III; WGR, Trp-Gly-Arg.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Nuclear Phosphatidylinositol 3,4,5-Trisphosphate Interactome Uncovers an Enrichment in Nucleolar Proteins

    doi: 10.1016/j.mcpro.2021.100102

    Figure Lengend Snippet: PARP1 binds to PPIn via three polybasic regions. A , domain structure of PARP1 and deletion constructs. B , schematic representation of lipids spotted (100 pmol) on PIP strips (Echelon Biosciences) including lysophosphatidic acid (LPA), lysophosphatidylcholine (LPC), phosphatidylinositol (PtdIns), PtdIns3 P , PtdIns4 P , PtdIns5 P , phosphatidylethanolamine (PE), phosphatidylcholine (PC), sphingosine-1-Phosphate (S1P), PtdIns(3,4) P 2 , PtdIns(3,5) P 2 , PtdIns(4,5) P 2 , PtdIns(3,4,5) P 3 , phosphatic acid (PA), phosphatidylserine (PS), and blank. C and D , lipid overlay assay using PIP strips incubated with recombinant GST-PARP1 deletion constructs WT and mutants and detection of protein–lipid interactions using an anti-GST-HRP–conjugated antibody. E , multiple sequence alignment of the polybasic regions located in the zinc finger III and the BRCT-WGR linker found in human PARP-1 compared with other vertebrate species performed using the online program MUSCLE. Accession number for Homo sapiens (P09874), Mus musculus (P11103), Bos taurus (P18493), Gallus gallus (P26446), Xenopus laevis (P31669), and Danio rerio (Q5RHR0). F , ribbon representation of the human PARP-1 zinc-finger III NMR structure (aa 233–357 PDB: 2JVN, ). The two polybasic regions found in the N-terminal (aa 233–236) and C-terminal (aa 346–352) parts of the zinc-finger III are colored in red . ART, (ADP-ribosyl) transferase domain (ART); BRCT, BRCA1 C-terminal domain; HD, helical subdomain; HRP, horse radish peroxidase; PARP1, poly(ADP-ribose) polymerase 1; ZnF 1 to 3, zinc-finger I-III; WGR, Trp-Gly-Arg.

    Article Snippet: B , schematic representation of lipids spotted (100 pmol) on PIP strips (Echelon Biosciences) including lysophosphatidic acid (LPA), lysophosphatidylcholine (LPC), phosphatidylinositol (PtdIns), PtdIns3 P , PtdIns4 P , PtdIns5 P , phosphatidylethanolamine (PE), phosphatidylcholine (PC), sphingosine-1-Phosphate (S1P), PtdIns(3,4) P 2 , PtdIns(3,5) P 2 , PtdIns(4,5) P 2 , PtdIns(3,4,5) P 3 , phosphatic acid (PA), phosphatidylserine (PS), and blank.

    Techniques: Construct, Overlay Assay, Incubation, Recombinant, Sequencing