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  • 94
    PerkinElmer s methionine
    S Methionine, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 94/100, based on 2603 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer s radiolabeled methionine
    The degradation of ELK-1 is proteasome-dependent and mediated by FBXO25. The methionine chase labeling experiments were performed using HEK293T cells transfected with the indicated plasmids. After the indicated times, the supernatants were obtained and submitted to immunoprecipitation ( IP ) with anti-ELK-1 antibody. The immunoprecipitated proteins were visualized by Coomassie Blue, and dried gels were film-exposed to protein visualization. A , FBXO25 and not pcDNA3 mediated ELK-1 degradation. B , 35 S-labeled ELK-1-FLAG-His 6 is degraded in the presence of FBXO25 after 12 h of transfection but not by pcDNA3 as indicated in densitometry graphics. C , 35 S-labeled ELK-1 degradation mediated by FBXO25 is proteasome-dependent. D , the proteasome inhibitor epoxomicin protected 35 S-labeled ELK-1-FLAG-His 6 of degradation by FBXO25 as indicated in densitometry graphics. For all experiments n = 2. H.C ., heavy chain. *, the apparent molecular mass of the <t>radiolabeled</t> band ELK-1-FLAG-His 6 on the gels was ascertained by comparison with the mobility of FLAG affinity-purified ELK-1-FLAG-His 6 (data not shown).
    S Radiolabeled Methionine, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 90/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    PerkinElmer s methionine cysteine
    The degradation of ELK-1 is proteasome-dependent and mediated by FBXO25. The methionine chase labeling experiments were performed using HEK293T cells transfected with the indicated plasmids. After the indicated times, the supernatants were obtained and submitted to immunoprecipitation ( IP ) with anti-ELK-1 antibody. The immunoprecipitated proteins were visualized by Coomassie Blue, and dried gels were film-exposed to protein visualization. A , FBXO25 and not pcDNA3 mediated ELK-1 degradation. B , 35 S-labeled ELK-1-FLAG-His 6 is degraded in the presence of FBXO25 after 12 h of transfection but not by pcDNA3 as indicated in densitometry graphics. C , 35 S-labeled ELK-1 degradation mediated by FBXO25 is proteasome-dependent. D , the proteasome inhibitor epoxomicin protected 35 S-labeled ELK-1-FLAG-His 6 of degradation by FBXO25 as indicated in densitometry graphics. For all experiments n = 2. H.C ., heavy chain. *, the apparent molecular mass of the <t>radiolabeled</t> band ELK-1-FLAG-His 6 on the gels was ascertained by comparison with the mobility of FLAG affinity-purified ELK-1-FLAG-His 6 (data not shown).
    S Methionine Cysteine, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 93/100, based on 1506 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    PerkinElmer s labeled methionine
    The degradation of ELK-1 is proteasome-dependent and mediated by FBXO25. The methionine chase labeling experiments were performed using HEK293T cells transfected with the indicated plasmids. After the indicated times, the supernatants were obtained and submitted to immunoprecipitation ( IP ) with anti-ELK-1 antibody. The immunoprecipitated proteins were visualized by Coomassie Blue, and dried gels were film-exposed to protein visualization. A , FBXO25 and not pcDNA3 mediated ELK-1 degradation. B , 35 S-labeled ELK-1-FLAG-His 6 is degraded in the presence of FBXO25 after 12 h of transfection but not by pcDNA3 as indicated in densitometry graphics. C , 35 S-labeled ELK-1 degradation mediated by FBXO25 is proteasome-dependent. D , the proteasome inhibitor epoxomicin protected 35 S-labeled ELK-1-FLAG-His 6 of degradation by FBXO25 as indicated in densitometry graphics. For all experiments n = 2. H.C ., heavy chain. *, the apparent molecular mass of the <t>radiolabeled</t> band ELK-1-FLAG-His 6 on the gels was ascertained by comparison with the mobility of FLAG affinity-purified ELK-1-FLAG-His 6 (data not shown).
    S Labeled Methionine, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 91/100, based on 351 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer s35 methionine mixture
    The degradation of ELK-1 is proteasome-dependent and mediated by FBXO25. The methionine chase labeling experiments were performed using HEK293T cells transfected with the indicated plasmids. After the indicated times, the supernatants were obtained and submitted to immunoprecipitation ( IP ) with anti-ELK-1 antibody. The immunoprecipitated proteins were visualized by Coomassie Blue, and dried gels were film-exposed to protein visualization. A , FBXO25 and not pcDNA3 mediated ELK-1 degradation. B , 35 S-labeled ELK-1-FLAG-His 6 is degraded in the presence of FBXO25 after 12 h of transfection but not by pcDNA3 as indicated in densitometry graphics. C , 35 S-labeled ELK-1 degradation mediated by FBXO25 is proteasome-dependent. D , the proteasome inhibitor epoxomicin protected 35 S-labeled ELK-1-FLAG-His 6 of degradation by FBXO25 as indicated in densitometry graphics. For all experiments n = 2. H.C ., heavy chain. *, the apparent molecular mass of the <t>radiolabeled</t> band ELK-1-FLAG-His 6 on the gels was ascertained by comparison with the mobility of FLAG affinity-purified ELK-1-FLAG-His 6 (data not shown).
    S35 Methionine Mixture, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    PerkinElmer s methionine 35 s cysteine expre35 s35 s protein labeling mix
    The degradation of ELK-1 is proteasome-dependent and mediated by FBXO25. The methionine chase labeling experiments were performed using HEK293T cells transfected with the indicated plasmids. After the indicated times, the supernatants were obtained and submitted to immunoprecipitation ( IP ) with anti-ELK-1 antibody. The immunoprecipitated proteins were visualized by Coomassie Blue, and dried gels were film-exposed to protein visualization. A , FBXO25 and not pcDNA3 mediated ELK-1 degradation. B , 35 S-labeled ELK-1-FLAG-His 6 is degraded in the presence of FBXO25 after 12 h of transfection but not by pcDNA3 as indicated in densitometry graphics. C , 35 S-labeled ELK-1 degradation mediated by FBXO25 is proteasome-dependent. D , the proteasome inhibitor epoxomicin protected 35 S-labeled ELK-1-FLAG-His 6 of degradation by FBXO25 as indicated in densitometry graphics. For all experiments n = 2. H.C ., heavy chain. *, the apparent molecular mass of the <t>radiolabeled</t> band ELK-1-FLAG-His 6 on the gels was ascertained by comparison with the mobility of FLAG affinity-purified ELK-1-FLAG-His 6 (data not shown).
    S Methionine 35 S Cysteine Expre35 S35 S Protein Labeling Mix, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    PerkinElmer easytag express 35 s
    The degradation of ELK-1 is proteasome-dependent and mediated by FBXO25. The methionine chase labeling experiments were performed using HEK293T cells transfected with the indicated plasmids. After the indicated times, the supernatants were obtained and submitted to immunoprecipitation ( IP ) with anti-ELK-1 antibody. The immunoprecipitated proteins were visualized by Coomassie Blue, and dried gels were film-exposed to protein visualization. A , FBXO25 and not pcDNA3 mediated ELK-1 degradation. B , 35 S-labeled ELK-1-FLAG-His 6 is degraded in the presence of FBXO25 after 12 h of transfection but not by pcDNA3 as indicated in densitometry graphics. C , 35 S-labeled ELK-1 degradation mediated by FBXO25 is proteasome-dependent. D , the proteasome inhibitor epoxomicin protected 35 S-labeled ELK-1-FLAG-His 6 of degradation by FBXO25 as indicated in densitometry graphics. For all experiments n = 2. H.C ., heavy chain. *, the apparent molecular mass of the <t>radiolabeled</t> band ELK-1-FLAG-His 6 on the gels was ascertained by comparison with the mobility of FLAG affinity-purified ELK-1-FLAG-His 6 (data not shown).
    Easytag Express 35 S, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 98/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    PerkinElmer s cysteine
    The degradation of ELK-1 is proteasome-dependent and mediated by FBXO25. The methionine chase labeling experiments were performed using HEK293T cells transfected with the indicated plasmids. After the indicated times, the supernatants were obtained and submitted to immunoprecipitation ( IP ) with anti-ELK-1 antibody. The immunoprecipitated proteins were visualized by Coomassie Blue, and dried gels were film-exposed to protein visualization. A , FBXO25 and not pcDNA3 mediated ELK-1 degradation. B , 35 S-labeled ELK-1-FLAG-His 6 is degraded in the presence of FBXO25 after 12 h of transfection but not by pcDNA3 as indicated in densitometry graphics. C , 35 S-labeled ELK-1 degradation mediated by FBXO25 is proteasome-dependent. D , the proteasome inhibitor epoxomicin protected 35 S-labeled ELK-1-FLAG-His 6 of degradation by FBXO25 as indicated in densitometry graphics. For all experiments n = 2. H.C ., heavy chain. *, the apparent molecular mass of the <t>radiolabeled</t> band ELK-1-FLAG-His 6 on the gels was ascertained by comparison with the mobility of FLAG affinity-purified ELK-1-FLAG-His 6 (data not shown).
    S Cysteine, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 93/100, based on 658 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    The degradation of ELK-1 is proteasome-dependent and mediated by FBXO25. The methionine chase labeling experiments were performed using HEK293T cells transfected with the indicated plasmids. After the indicated times, the supernatants were obtained and submitted to immunoprecipitation ( IP ) with anti-ELK-1 antibody. The immunoprecipitated proteins were visualized by Coomassie Blue, and dried gels were film-exposed to protein visualization. A , FBXO25 and not pcDNA3 mediated ELK-1 degradation. B , 35 S-labeled ELK-1-FLAG-His 6 is degraded in the presence of FBXO25 after 12 h of transfection but not by pcDNA3 as indicated in densitometry graphics. C , 35 S-labeled ELK-1 degradation mediated by FBXO25 is proteasome-dependent. D , the proteasome inhibitor epoxomicin protected 35 S-labeled ELK-1-FLAG-His 6 of degradation by FBXO25 as indicated in densitometry graphics. For all experiments n = 2. H.C ., heavy chain. *, the apparent molecular mass of the radiolabeled band ELK-1-FLAG-His 6 on the gels was ascertained by comparison with the mobility of FLAG affinity-purified ELK-1-FLAG-His 6 (data not shown).

    Journal: The Journal of Biological Chemistry

    Article Title: The F-box Protein FBXO25 Promotes the Proteasome-dependent Degradation of ELK-1 Protein *

    doi: 10.1074/jbc.M113.504308

    Figure Lengend Snippet: The degradation of ELK-1 is proteasome-dependent and mediated by FBXO25. The methionine chase labeling experiments were performed using HEK293T cells transfected with the indicated plasmids. After the indicated times, the supernatants were obtained and submitted to immunoprecipitation ( IP ) with anti-ELK-1 antibody. The immunoprecipitated proteins were visualized by Coomassie Blue, and dried gels were film-exposed to protein visualization. A , FBXO25 and not pcDNA3 mediated ELK-1 degradation. B , 35 S-labeled ELK-1-FLAG-His 6 is degraded in the presence of FBXO25 after 12 h of transfection but not by pcDNA3 as indicated in densitometry graphics. C , 35 S-labeled ELK-1 degradation mediated by FBXO25 is proteasome-dependent. D , the proteasome inhibitor epoxomicin protected 35 S-labeled ELK-1-FLAG-His 6 of degradation by FBXO25 as indicated in densitometry graphics. For all experiments n = 2. H.C ., heavy chain. *, the apparent molecular mass of the radiolabeled band ELK-1-FLAG-His 6 on the gels was ascertained by comparison with the mobility of FLAG affinity-purified ELK-1-FLAG-His 6 (data not shown).

    Article Snippet: After 24 h, the cells were washed with PBS and DMEM without methionine (Sigma-Aldrich) and kept in this medium for 2 h. Then the cells were pulsed for 1 h with 150 μCi/well 35 S-radiolabeled methionine (Express labeling mix 35 S; PerkinElmer Life Sciences).

    Techniques: Labeling, Transfection, Immunoprecipitation, Affinity Purification