s enterica subsp enterica serotype enteritidis Search Results


s enterica serotype enteritidis  (Millipore)
86
Millipore s enterica serotype enteritidis
Evans blue (EB) permeability into the brains of LPS-treated mice. Two different kinds of lipopolysaccharides (LPSs) derived from <t>Salmonella</t> <t>enterica</t> [LPS(S)] and Escherichia coli [LPS(E)] were tested in two different strains of mice (C57BL/6 [B6] and ICR). The intraperitoneally administered EB (4 mL/kg, 2% [w/v] in phosphate buffered saline [PBS]; pH 7.4) appeared in the homogenized brains following the injection of LPS(S) or LPS(E) in the different mice strains. Compared to the control group administered with vehicle (PBS) only, the amount of EB that penetrated the brain was significantly higher in both mouse strains after injection with both LPSs. Interestingly, for both LPSs the amounts of EB penetrating ICR mouse brains were greater than those of B6 mouse brains, and for both mouse strains the amount of EB penetrance was greater in LPS(S) than in LPS(E). * p < 0.05 ICR/LPS(S) vs . ICR/LPS(E), † p < 0.005 vs . vehicle group (PBS), ‡ p < 0.001 vs . vehicle group (PBS).
S Enterica Serotype Enteritidis, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s enterica serotype enteritidis  (Tubex GmbH)
86
Tubex GmbH s enterica serotype enteritidis
Evans blue (EB) permeability into the brains of LPS-treated mice. Two different kinds of lipopolysaccharides (LPSs) derived from <t>Salmonella</t> <t>enterica</t> [LPS(S)] and Escherichia coli [LPS(E)] were tested in two different strains of mice (C57BL/6 [B6] and ICR). The intraperitoneally administered EB (4 mL/kg, 2% [w/v] in phosphate buffered saline [PBS]; pH 7.4) appeared in the homogenized brains following the injection of LPS(S) or LPS(E) in the different mice strains. Compared to the control group administered with vehicle (PBS) only, the amount of EB that penetrated the brain was significantly higher in both mouse strains after injection with both LPSs. Interestingly, for both LPSs the amounts of EB penetrating ICR mouse brains were greater than those of B6 mouse brains, and for both mouse strains the amount of EB penetrance was greater in LPS(S) than in LPS(E). * p < 0.05 ICR/LPS(S) vs . ICR/LPS(E), † p < 0.005 vs . vehicle group (PBS), ‡ p < 0.001 vs . vehicle group (PBS).
S Enterica Serotype Enteritidis, supplied by Tubex GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s enterica serovar enteritidis lps  (Millipore)
86
Millipore s enterica serovar enteritidis lps
Evans blue (EB) permeability into the brains of LPS-treated mice. Two different kinds of lipopolysaccharides (LPSs) derived from <t>Salmonella</t> <t>enterica</t> [LPS(S)] and Escherichia coli [LPS(E)] were tested in two different strains of mice (C57BL/6 [B6] and ICR). The intraperitoneally administered EB (4 mL/kg, 2% [w/v] in phosphate buffered saline [PBS]; pH 7.4) appeared in the homogenized brains following the injection of LPS(S) or LPS(E) in the different mice strains. Compared to the control group administered with vehicle (PBS) only, the amount of EB that penetrated the brain was significantly higher in both mouse strains after injection with both LPSs. Interestingly, for both LPSs the amounts of EB penetrating ICR mouse brains were greater than those of B6 mouse brains, and for both mouse strains the amount of EB penetrance was greater in LPS(S) than in LPS(E). * p < 0.05 ICR/LPS(S) vs . ICR/LPS(E), † p < 0.005 vs . vehicle group (PBS), ‡ p < 0.001 vs . vehicle group (PBS).
S Enterica Serovar Enteritidis Lps, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s enterica serotype enteritidis e beckman coulter ceq  (Beckman Coulter)
86
Beckman Coulter s enterica serotype enteritidis e beckman coulter ceq
Evans blue (EB) permeability into the brains of LPS-treated mice. Two different kinds of lipopolysaccharides (LPSs) derived from <t>Salmonella</t> <t>enterica</t> [LPS(S)] and Escherichia coli [LPS(E)] were tested in two different strains of mice (C57BL/6 [B6] and ICR). The intraperitoneally administered EB (4 mL/kg, 2% [w/v] in phosphate buffered saline [PBS]; pH 7.4) appeared in the homogenized brains following the injection of LPS(S) or LPS(E) in the different mice strains. Compared to the control group administered with vehicle (PBS) only, the amount of EB that penetrated the brain was significantly higher in both mouse strains after injection with both LPSs. Interestingly, for both LPSs the amounts of EB penetrating ICR mouse brains were greater than those of B6 mouse brains, and for both mouse strains the amount of EB penetrance was greater in LPS(S) than in LPS(E). * p < 0.05 ICR/LPS(S) vs . ICR/LPS(E), † p < 0.005 vs . vehicle group (PBS), ‡ p < 0.001 vs . vehicle group (PBS).
S Enterica Serotype Enteritidis E Beckman Coulter Ceq, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Evans blue (EB) permeability into the brains of LPS-treated mice. Two different kinds of lipopolysaccharides (LPSs) derived from Salmonella enterica [LPS(S)] and Escherichia coli [LPS(E)] were tested in two different strains of mice (C57BL/6 [B6] and ICR). The intraperitoneally administered EB (4 mL/kg, 2% [w/v] in phosphate buffered saline [PBS]; pH 7.4) appeared in the homogenized brains following the injection of LPS(S) or LPS(E) in the different mice strains. Compared to the control group administered with vehicle (PBS) only, the amount of EB that penetrated the brain was significantly higher in both mouse strains after injection with both LPSs. Interestingly, for both LPSs the amounts of EB penetrating ICR mouse brains were greater than those of B6 mouse brains, and for both mouse strains the amount of EB penetrance was greater in LPS(S) than in LPS(E). * p < 0.05 ICR/LPS(S) vs . ICR/LPS(E), † p < 0.005 vs . vehicle group (PBS), ‡ p < 0.001 vs . vehicle group (PBS).

Journal: Journal of Veterinary Science

Article Title: Establishment of minimal positive-control conditions to ensure brain safety during rapid development of emergency vaccines

doi: 10.4142/jvs.2017.18.S1.371

Figure Lengend Snippet: Evans blue (EB) permeability into the brains of LPS-treated mice. Two different kinds of lipopolysaccharides (LPSs) derived from Salmonella enterica [LPS(S)] and Escherichia coli [LPS(E)] were tested in two different strains of mice (C57BL/6 [B6] and ICR). The intraperitoneally administered EB (4 mL/kg, 2% [w/v] in phosphate buffered saline [PBS]; pH 7.4) appeared in the homogenized brains following the injection of LPS(S) or LPS(E) in the different mice strains. Compared to the control group administered with vehicle (PBS) only, the amount of EB that penetrated the brain was significantly higher in both mouse strains after injection with both LPSs. Interestingly, for both LPSs the amounts of EB penetrating ICR mouse brains were greater than those of B6 mouse brains, and for both mouse strains the amount of EB penetrance was greater in LPS(S) than in LPS(E). * p < 0.05 ICR/LPS(S) vs . ICR/LPS(E), † p < 0.005 vs . vehicle group (PBS), ‡ p < 0.001 vs . vehicle group (PBS).

Article Snippet: The LPSs from S. enterica serotype enteritidis (L6011; Sigma, USA) and E. coli O55:B5 (L2880; Sigma) were separately dissolved in phosphate buffered saline (PBS; pH 7.4) to a concentration of 1 mg/4 mL.

Techniques: Permeability, Derivative Assay, Injection

How to apply the lipopolysaccharide (LPS) positive-control protocol to verify vaccine safety at the blood-brain barrier (BBB). Based on the results of our experiments, we have established a positive-control protocol for determining vaccine safety at the BBB. Salmonella enterica -derived LPS and vaccine are intraperitoneally injected into 7-week-old ICR mice (1.0 mg LPS/4 mL PBS/kg body weight) and brain hemispheres are removed after 4 h. The mRNA is extracted, followed by quantitative real-time polymerase chain reaction (PCR) using zonula occludens-1 (ZO-1), occludin, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) primers. By comparing the tight junction mRNA levels of the vaccine-injected group with those of the LPS-injected group, the potential danger to the BBB of the vaccine is evaluated.

Journal: Journal of Veterinary Science

Article Title: Establishment of minimal positive-control conditions to ensure brain safety during rapid development of emergency vaccines

doi: 10.4142/jvs.2017.18.S1.371

Figure Lengend Snippet: How to apply the lipopolysaccharide (LPS) positive-control protocol to verify vaccine safety at the blood-brain barrier (BBB). Based on the results of our experiments, we have established a positive-control protocol for determining vaccine safety at the BBB. Salmonella enterica -derived LPS and vaccine are intraperitoneally injected into 7-week-old ICR mice (1.0 mg LPS/4 mL PBS/kg body weight) and brain hemispheres are removed after 4 h. The mRNA is extracted, followed by quantitative real-time polymerase chain reaction (PCR) using zonula occludens-1 (ZO-1), occludin, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) primers. By comparing the tight junction mRNA levels of the vaccine-injected group with those of the LPS-injected group, the potential danger to the BBB of the vaccine is evaluated.

Article Snippet: The LPSs from S. enterica serotype enteritidis (L6011; Sigma, USA) and E. coli O55:B5 (L2880; Sigma) were separately dissolved in phosphate buffered saline (PBS; pH 7.4) to a concentration of 1 mg/4 mL.

Techniques: Positive Control, Derivative Assay, Injection, Real-time Polymerase Chain Reaction