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  • 94
    ATCC ruminococcus flavefaciens
    Sequences used for the design of the cel5 , cel48 , hydA , dsrA , and mcrA primer sets
    Ruminococcus Flavefaciens, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ruminococcus flavefaciens/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    86
    Addgene inc ruminococcus flavefaciens
    pgRNAs can robustly suppress viral spread in higher organisms ( Nicotiana benthamiana ). (A) A pgRNA for <t>RfxCas13d</t> was designed to target two sequences in a tobacco mosaic virus (TMV) variant replicon (TRBO-GFP) genome. The targets had 30% (6 out of 23) sequence divergence. MP: movement protein. GFP: green fluorescent protein. (B) Leaves were infiltrated with a suspension of A. tumerfaciens harbouring plasmids for the transient expression of RfxCas13d; a pgRNA, its two “monovalent” counterpart gRNAs, or a non-targeting (NT) gRNA; and replication-competent TRBO-GFP. Representative images of leaves illuminated under UV light three days after infiltration show the extent of viral spread by GFP expression; see Figure S9 for additional leaf images. Viral spread is suppressed by Cas13 RNPs with gRNAs and strongly by Cas13 RNPs with pgRNAs, but not Cas13 RNPs with a non-targeting (NT) gRNA. (C) Quantitative reverse-transcription PCR (qRT-PCR) of leaf RNA after transient expression demonstrates that pgRNAs are capable of more effectively reducing viral RNA levels than their monovalent counterparts in a higher organism (two-sided T-test; * p < 0.05, ** p < 0.01). N = 4 leaves each for gRNA.
    Ruminococcus Flavefaciens, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ruminococcus flavefaciens/product/Addgene inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ruminococcus flavefaciens - by Bioz Stars, 2023-02
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    86
    DSMZ ruminococcus flavefaciens
    pgRNAs can robustly suppress viral spread in higher organisms ( Nicotiana benthamiana ). (A) A pgRNA for <t>RfxCas13d</t> was designed to target two sequences in a tobacco mosaic virus (TMV) variant replicon (TRBO-GFP) genome. The targets had 30% (6 out of 23) sequence divergence. MP: movement protein. GFP: green fluorescent protein. (B) Leaves were infiltrated with a suspension of A. tumerfaciens harbouring plasmids for the transient expression of RfxCas13d; a pgRNA, its two “monovalent” counterpart gRNAs, or a non-targeting (NT) gRNA; and replication-competent TRBO-GFP. Representative images of leaves illuminated under UV light three days after infiltration show the extent of viral spread by GFP expression; see Figure S9 for additional leaf images. Viral spread is suppressed by Cas13 RNPs with gRNAs and strongly by Cas13 RNPs with pgRNAs, but not Cas13 RNPs with a non-targeting (NT) gRNA. (C) Quantitative reverse-transcription PCR (qRT-PCR) of leaf RNA after transient expression demonstrates that pgRNAs are capable of more effectively reducing viral RNA levels than their monovalent counterparts in a higher organism (two-sided T-test; * p < 0.05, ** p < 0.01). N = 4 leaves each for gRNA.
    Ruminococcus Flavefaciens, supplied by DSMZ, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ruminococcus flavefaciens/product/DSMZ
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ruminococcus flavefaciens - by Bioz Stars, 2023-02
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    Image Search Results


    Sequences used for the design of the cel5 , cel48 , hydA , dsrA , and mcrA primer sets

    Journal: Applied and Environmental Microbiology

    Article Title: Detection and Quantification of Functional Genes of Cellulose- Degrading, Fermentative, and Sulfate-Reducing Bacteria and Methanogenic Archaea

    doi: 10.1128/AEM.01285-09

    Figure Lengend Snippet: Sequences used for the design of the cel5 , cel48 , hydA , dsrA , and mcrA primer sets

    Article Snippet: Pure cultures of the following microorganisms were grown in the laboratory, and their genomic DNAs were used as positive or negative controls for PCR: Escherichia coli , Clostridium cellulovorans (ATCC 35296), Ruminococcus flavefaciens (ATCC 49949), and Fibrobacter succinogenes strain B1 (ATCC 51214).

    Techniques:

    pgRNAs can robustly suppress viral spread in higher organisms ( Nicotiana benthamiana ). (A) A pgRNA for RfxCas13d was designed to target two sequences in a tobacco mosaic virus (TMV) variant replicon (TRBO-GFP) genome. The targets had 30% (6 out of 23) sequence divergence. MP: movement protein. GFP: green fluorescent protein. (B) Leaves were infiltrated with a suspension of A. tumerfaciens harbouring plasmids for the transient expression of RfxCas13d; a pgRNA, its two “monovalent” counterpart gRNAs, or a non-targeting (NT) gRNA; and replication-competent TRBO-GFP. Representative images of leaves illuminated under UV light three days after infiltration show the extent of viral spread by GFP expression; see Figure S9 for additional leaf images. Viral spread is suppressed by Cas13 RNPs with gRNAs and strongly by Cas13 RNPs with pgRNAs, but not Cas13 RNPs with a non-targeting (NT) gRNA. (C) Quantitative reverse-transcription PCR (qRT-PCR) of leaf RNA after transient expression demonstrates that pgRNAs are capable of more effectively reducing viral RNA levels than their monovalent counterparts in a higher organism (two-sided T-test; * p < 0.05, ** p < 0.01). N = 4 leaves each for gRNA.

    Journal: bioRxiv

    Article Title: Polyvalent Guide RNAs for CRISPR Antivirals

    doi: 10.1101/2021.02.25.430352

    Figure Lengend Snippet: pgRNAs can robustly suppress viral spread in higher organisms ( Nicotiana benthamiana ). (A) A pgRNA for RfxCas13d was designed to target two sequences in a tobacco mosaic virus (TMV) variant replicon (TRBO-GFP) genome. The targets had 30% (6 out of 23) sequence divergence. MP: movement protein. GFP: green fluorescent protein. (B) Leaves were infiltrated with a suspension of A. tumerfaciens harbouring plasmids for the transient expression of RfxCas13d; a pgRNA, its two “monovalent” counterpart gRNAs, or a non-targeting (NT) gRNA; and replication-competent TRBO-GFP. Representative images of leaves illuminated under UV light three days after infiltration show the extent of viral spread by GFP expression; see Figure S9 for additional leaf images. Viral spread is suppressed by Cas13 RNPs with gRNAs and strongly by Cas13 RNPs with pgRNAs, but not Cas13 RNPs with a non-targeting (NT) gRNA. (C) Quantitative reverse-transcription PCR (qRT-PCR) of leaf RNA after transient expression demonstrates that pgRNAs are capable of more effectively reducing viral RNA levels than their monovalent counterparts in a higher organism (two-sided T-test; * p < 0.05, ** p < 0.01). N = 4 leaves each for gRNA.

    Article Snippet: The DNA sequences of the plant codon optimized Cas13d-EGFP with the Cas13d from Ruminococcus flavefaciens (RfxCas13d) flanked by two nuclear localization signal (NLS) was amplified from plasmid pXR001 (Addgene #109049) using Q5 high fidelity of DNA polymerase (NEB).

    Techniques: Variant Assay, Sequencing, Expressing, Quantitative RT-PCR