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  • 93
    CLS Cell Lines Service GmbH rt112 cells
    Rt112 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rt112 cells/product/CLS Cell Lines Service GmbH
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rt112 cells - by Bioz Stars, 2024-06
    93/100 stars
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    86
    Millipore rt112 cells
    ( A and B ) Total proteins extracted from BC cell line T24 that was stably transduced with control shRNA (shCT) or with shRNA of PKM2 (shPKM2) were incubated with solid-phase shikonin. After pull-down (PD), bound proteins were eluted with SDS-sample buffer, resolved on SDS-PAGE and stained with silver nitrate or immuno-blotted with various antibodies as indicated. Note, upon Western blotting (WB) of GAPDH, the equal amounts of input total proteins in the shCT cells and shPKM2 cells ( A , top panel); the presence of a 55-kDa protein species ( A , middle panel; arrow) only in the shCT cells; and the strong reactivity of this protein with anti-PKM2 antibody on Western blotting (lower panel). The result was reproducible in another pull-down experiment followed by Western blotting ( B ) showing the pull-down of PKM2, but not PKM1, MAPK or AKT, by solid-phase shikonin. Some gel and blot images were cropped to save space and their full-length versions are available upon request. ( C ) BC cell lines T24 and <t>RT112</t> were incubated in the culture media containing various concentrations of shikonin and their proliferation status was assessed by WST-1 assay at the time points indicated. Note the dose- and time-dependent inhibition of cell proliferation by shikonin. ( D ) T24 cells stably transduced with shCT, shPKM1 and shPKM2 were incubated with the culture media containing 0.5 μM shikonin for 24 hours and assayed by WST-1. Note that the down-regulation of PKM2, but not that of PKM1, blunted the inhibitory effect of shikonin. ( E ) Cultured T24 and RT112 cells were treated with DMSO, 0.5 μM shikonin or 5 μM shikonin and the equal amounts of cell lysates were subject to pyruvate kinase activity assay. Note that, at the concentration that markedly inhibited cell proliferation (0.5 μM; panel C ), shikonin did not significantly affect the pyruvate kinase activity ( E ).
    Rt112 Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rt112 cells/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rt112 cells - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Thermo Fisher rt112 cells
    ( A and B ) Total proteins extracted from BC cell line T24 that was stably transduced with control shRNA (shCT) or with shRNA of PKM2 (shPKM2) were incubated with solid-phase shikonin. After pull-down (PD), bound proteins were eluted with SDS-sample buffer, resolved on SDS-PAGE and stained with silver nitrate or immuno-blotted with various antibodies as indicated. Note, upon Western blotting (WB) of GAPDH, the equal amounts of input total proteins in the shCT cells and shPKM2 cells ( A , top panel); the presence of a 55-kDa protein species ( A , middle panel; arrow) only in the shCT cells; and the strong reactivity of this protein with anti-PKM2 antibody on Western blotting (lower panel). The result was reproducible in another pull-down experiment followed by Western blotting ( B ) showing the pull-down of PKM2, but not PKM1, MAPK or AKT, by solid-phase shikonin. Some gel and blot images were cropped to save space and their full-length versions are available upon request. ( C ) BC cell lines T24 and <t>RT112</t> were incubated in the culture media containing various concentrations of shikonin and their proliferation status was assessed by WST-1 assay at the time points indicated. Note the dose- and time-dependent inhibition of cell proliferation by shikonin. ( D ) T24 cells stably transduced with shCT, shPKM1 and shPKM2 were incubated with the culture media containing 0.5 μM shikonin for 24 hours and assayed by WST-1. Note that the down-regulation of PKM2, but not that of PKM1, blunted the inhibitory effect of shikonin. ( E ) Cultured T24 and RT112 cells were treated with DMSO, 0.5 μM shikonin or 5 μM shikonin and the equal amounts of cell lysates were subject to pyruvate kinase activity assay. Note that, at the concentration that markedly inhibited cell proliferation (0.5 μM; panel C ), shikonin did not significantly affect the pyruvate kinase activity ( E ).
    Rt112 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rt112 cells/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rt112 cells - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    DSMZ rt112 cells
    ( A and B ) Total proteins extracted from BC cell line T24 that was stably transduced with control shRNA (shCT) or with shRNA of PKM2 (shPKM2) were incubated with solid-phase shikonin. After pull-down (PD), bound proteins were eluted with SDS-sample buffer, resolved on SDS-PAGE and stained with silver nitrate or immuno-blotted with various antibodies as indicated. Note, upon Western blotting (WB) of GAPDH, the equal amounts of input total proteins in the shCT cells and shPKM2 cells ( A , top panel); the presence of a 55-kDa protein species ( A , middle panel; arrow) only in the shCT cells; and the strong reactivity of this protein with anti-PKM2 antibody on Western blotting (lower panel). The result was reproducible in another pull-down experiment followed by Western blotting ( B ) showing the pull-down of PKM2, but not PKM1, MAPK or AKT, by solid-phase shikonin. Some gel and blot images were cropped to save space and their full-length versions are available upon request. ( C ) BC cell lines T24 and <t>RT112</t> were incubated in the culture media containing various concentrations of shikonin and their proliferation status was assessed by WST-1 assay at the time points indicated. Note the dose- and time-dependent inhibition of cell proliferation by shikonin. ( D ) T24 cells stably transduced with shCT, shPKM1 and shPKM2 were incubated with the culture media containing 0.5 μM shikonin for 24 hours and assayed by WST-1. Note that the down-regulation of PKM2, but not that of PKM1, blunted the inhibitory effect of shikonin. ( E ) Cultured T24 and RT112 cells were treated with DMSO, 0.5 μM shikonin or 5 μM shikonin and the equal amounts of cell lysates were subject to pyruvate kinase activity assay. Note that, at the concentration that markedly inhibited cell proliferation (0.5 μM; panel C ), shikonin did not significantly affect the pyruvate kinase activity ( E ).
    Rt112 Cells, supplied by DSMZ, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rt112 cells/product/DSMZ
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rt112 cells - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Galectin Therapeutics rt112 cells
    Anticancer activities of pectin extracted from different sources.
    Rt112 Cells, supplied by Galectin Therapeutics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rt112 cells/product/Galectin Therapeutics
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rt112 cells - by Bioz Stars, 2024-06
    86/100 stars
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    Image Search Results


    ( A and B ) Total proteins extracted from BC cell line T24 that was stably transduced with control shRNA (shCT) or with shRNA of PKM2 (shPKM2) were incubated with solid-phase shikonin. After pull-down (PD), bound proteins were eluted with SDS-sample buffer, resolved on SDS-PAGE and stained with silver nitrate or immuno-blotted with various antibodies as indicated. Note, upon Western blotting (WB) of GAPDH, the equal amounts of input total proteins in the shCT cells and shPKM2 cells ( A , top panel); the presence of a 55-kDa protein species ( A , middle panel; arrow) only in the shCT cells; and the strong reactivity of this protein with anti-PKM2 antibody on Western blotting (lower panel). The result was reproducible in another pull-down experiment followed by Western blotting ( B ) showing the pull-down of PKM2, but not PKM1, MAPK or AKT, by solid-phase shikonin. Some gel and blot images were cropped to save space and their full-length versions are available upon request. ( C ) BC cell lines T24 and RT112 were incubated in the culture media containing various concentrations of shikonin and their proliferation status was assessed by WST-1 assay at the time points indicated. Note the dose- and time-dependent inhibition of cell proliferation by shikonin. ( D ) T24 cells stably transduced with shCT, shPKM1 and shPKM2 were incubated with the culture media containing 0.5 μM shikonin for 24 hours and assayed by WST-1. Note that the down-regulation of PKM2, but not that of PKM1, blunted the inhibitory effect of shikonin. ( E ) Cultured T24 and RT112 cells were treated with DMSO, 0.5 μM shikonin or 5 μM shikonin and the equal amounts of cell lysates were subject to pyruvate kinase activity assay. Note that, at the concentration that markedly inhibited cell proliferation (0.5 μM; panel C ), shikonin did not significantly affect the pyruvate kinase activity ( E ).

    Journal: Scientific Reports

    Article Title: Inhibition of Pyruvate Kinase M2 Markedly Reduces Chemoresistance of Advanced Bladder Cancer to Cisplatin

    doi: 10.1038/srep45983

    Figure Lengend Snippet: ( A and B ) Total proteins extracted from BC cell line T24 that was stably transduced with control shRNA (shCT) or with shRNA of PKM2 (shPKM2) were incubated with solid-phase shikonin. After pull-down (PD), bound proteins were eluted with SDS-sample buffer, resolved on SDS-PAGE and stained with silver nitrate or immuno-blotted with various antibodies as indicated. Note, upon Western blotting (WB) of GAPDH, the equal amounts of input total proteins in the shCT cells and shPKM2 cells ( A , top panel); the presence of a 55-kDa protein species ( A , middle panel; arrow) only in the shCT cells; and the strong reactivity of this protein with anti-PKM2 antibody on Western blotting (lower panel). The result was reproducible in another pull-down experiment followed by Western blotting ( B ) showing the pull-down of PKM2, but not PKM1, MAPK or AKT, by solid-phase shikonin. Some gel and blot images were cropped to save space and their full-length versions are available upon request. ( C ) BC cell lines T24 and RT112 were incubated in the culture media containing various concentrations of shikonin and their proliferation status was assessed by WST-1 assay at the time points indicated. Note the dose- and time-dependent inhibition of cell proliferation by shikonin. ( D ) T24 cells stably transduced with shCT, shPKM1 and shPKM2 were incubated with the culture media containing 0.5 μM shikonin for 24 hours and assayed by WST-1. Note that the down-regulation of PKM2, but not that of PKM1, blunted the inhibitory effect of shikonin. ( E ) Cultured T24 and RT112 cells were treated with DMSO, 0.5 μM shikonin or 5 μM shikonin and the equal amounts of cell lysates were subject to pyruvate kinase activity assay. Note that, at the concentration that markedly inhibited cell proliferation (0.5 μM; panel C ), shikonin did not significantly affect the pyruvate kinase activity ( E ).

    Article Snippet: The pyruvate kinase activity of shikonin-treated T24 and RT112 cells was measured using a kit from Sigma-Aldrich (St Louis, MO).

    Techniques: Stable Transfection, Transduction, shRNA, Incubation, SDS Page, Staining, Western Blot, WST-1 Assay, Inhibition, Cell Culture, Kinase Assay, Concentration Assay, Activity Assay

    ( A ) T24 cells were incubated with culture media containing DMSO as control (CT), 1 μg/ml cisplatin (CP), 1 μM shikonin (SK) or both for 72 hours and then subjected to WST-1 cell proliferation assay. ( B ) RT112 cells were treated similarly with the exception of shikonin concentration used at 0.25 μM. Note the marked inhibition of cell proliferation of both cell lines with the two-agent regimen compared to the one-agent regimen. Asterisks indicated the statistical significance between multi-group comparisons. ( C ) Western blotting showing the levels of PKM2 and PKM1 in T24 cells treated with DMSO, cisplatin (1 μg/ml), shikonin (1 μM) or both. Note the comparable levels of the proteins in different treatment groups. ( D ) Western blotting of treated T24 cells showing the upregulated levels of cleaved PARP and BiP (GRP78) in cisplatin-treated cells; cleaved caspase 3, cleaved caspase 7 and LC3-I in shikonin-treated cells; and all of these proteins in the dual-agent treatment group. The blot images were cropped to save space and their full-length versions are available upon request.

    Journal: Scientific Reports

    Article Title: Inhibition of Pyruvate Kinase M2 Markedly Reduces Chemoresistance of Advanced Bladder Cancer to Cisplatin

    doi: 10.1038/srep45983

    Figure Lengend Snippet: ( A ) T24 cells were incubated with culture media containing DMSO as control (CT), 1 μg/ml cisplatin (CP), 1 μM shikonin (SK) or both for 72 hours and then subjected to WST-1 cell proliferation assay. ( B ) RT112 cells were treated similarly with the exception of shikonin concentration used at 0.25 μM. Note the marked inhibition of cell proliferation of both cell lines with the two-agent regimen compared to the one-agent regimen. Asterisks indicated the statistical significance between multi-group comparisons. ( C ) Western blotting showing the levels of PKM2 and PKM1 in T24 cells treated with DMSO, cisplatin (1 μg/ml), shikonin (1 μM) or both. Note the comparable levels of the proteins in different treatment groups. ( D ) Western blotting of treated T24 cells showing the upregulated levels of cleaved PARP and BiP (GRP78) in cisplatin-treated cells; cleaved caspase 3, cleaved caspase 7 and LC3-I in shikonin-treated cells; and all of these proteins in the dual-agent treatment group. The blot images were cropped to save space and their full-length versions are available upon request.

    Article Snippet: The pyruvate kinase activity of shikonin-treated T24 and RT112 cells was measured using a kit from Sigma-Aldrich (St Louis, MO).

    Techniques: Incubation, Proliferation Assay, Concentration Assay, Inhibition, Western Blot

    BC cell lines NTUB1 ( A ), T24 ( B ) and RT112 ( C ) were passaged in media containing escalating concentrations of cisplatin as indicated. Both parental cell lines and cisplatin-resistant cell lines (indicated as/CP) obtained from the various concentrations of cisplatin were then assessed for their proliferation status by WST-1 assay (left panels of ( A – C )) and the levels of PKM2 by Western blotting (right panels of ( A – C )) at the end of 72 hours of subculture at 1 μg/ml cisplatin concentration point for all the three cell lines. Note the generation of cisplatin-resistant cell lines that were more viable in high concentrations of cisplatin for each of the three parental cell lines and the markedly increased PKM2 expression in these cisplatin-resistant cells compared to parental cells. The blot images were cropped to save space and their full-length versions are available upon request.

    Journal: Scientific Reports

    Article Title: Inhibition of Pyruvate Kinase M2 Markedly Reduces Chemoresistance of Advanced Bladder Cancer to Cisplatin

    doi: 10.1038/srep45983

    Figure Lengend Snippet: BC cell lines NTUB1 ( A ), T24 ( B ) and RT112 ( C ) were passaged in media containing escalating concentrations of cisplatin as indicated. Both parental cell lines and cisplatin-resistant cell lines (indicated as/CP) obtained from the various concentrations of cisplatin were then assessed for their proliferation status by WST-1 assay (left panels of ( A – C )) and the levels of PKM2 by Western blotting (right panels of ( A – C )) at the end of 72 hours of subculture at 1 μg/ml cisplatin concentration point for all the three cell lines. Note the generation of cisplatin-resistant cell lines that were more viable in high concentrations of cisplatin for each of the three parental cell lines and the markedly increased PKM2 expression in these cisplatin-resistant cells compared to parental cells. The blot images were cropped to save space and their full-length versions are available upon request.

    Article Snippet: The pyruvate kinase activity of shikonin-treated T24 and RT112 cells was measured using a kit from Sigma-Aldrich (St Louis, MO).

    Techniques: WST-1 Assay, Western Blot, Concentration Assay, Expressing

    Anticancer activities of pectin extracted from different sources.

    Journal: Heliyon

    Article Title: Pectin a multifaceted biopolymer in the management of cancer: A review

    doi: 10.1016/j.heliyon.2023.e22236

    Figure Lengend Snippet: Anticancer activities of pectin extracted from different sources.

    Article Snippet: Olive by-product , Uronic acid: 5.80–45.43 g/100 g sample, molecular weight: 500 and 2 kDa , Human bladder tumour cell lines (non-muscle invasive-RT112) and squamous-SCaBER) , SCaBER cells were the most sensitive to pectin samples (80 % reduction in cell proliferation) as compared to RT112 cells (20–40 % reduction in cell proliferation), slightly suppressed galectin-1 and galectin-3 protein expression, galectin inhibitory activity was evaluated using the hemagglutination assay and olive pectin samples showed potent agglutination inhibition at a minimum inhibitory concentration of 4–8 mg/mL. , The presence of phenols (16.80–72.30 g/100 g sample) could be related to the antiproliferative activity and hemagglutination inhibitory activity. , [ ] .

    Techniques: Activity Assay, Molecular Weight, Knock-Out, Inhibition, Concentration Assay, Modification, Activation Assay, Irradiation, Expressing, Isolation, Hemagglutination Assay, Agglutination, Methylation, Binding Assay, Cell Attachment Assay, Recombinant