rt-pcr Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher reverse transcription polymerase chain reaction rt pcr
    Reverse Transcription Polymerase Chain Reaction Rt Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 587 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription polymerase chain reaction rt pcr/product/Thermo Fisher
    Average 99 stars, based on 587 article reviews
    Price from $9.99 to $1999.99
    reverse transcription polymerase chain reaction rt pcr - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Millipore reverse transcription polymerase chain reaction rt pcr
    Reverse Transcription Polymerase Chain Reaction Rt Pcr, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription polymerase chain reaction rt pcr/product/Millipore
    Average 99 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    reverse transcription polymerase chain reaction rt pcr - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    90
    TaKaRa reverse transcription polymerase chain reaction rt pcr kit
    Changes in BCL-2 family expression caused by rapamycin treatment and FAK down-regulation. REH-empty vector or REH-FAK shRNA cells were cultured with or without rapamycin (100 nM). After 10 h, <t>RNA</t> was extracted from these cells and mRNA expression was detected using quantitative real-time <t>PCR.</t> The expression of pro-apoptosis genes, such as BIK, PUMA, BMF, BAX, and MCL-1S, was higher in the combination treatment group than in the rapamycin-only group. The expression of anti-apoptosis genes, such as BCL-2, was decreased in the combination treatment group compared with the rapamycin-only group. The results represent the mean ± S.D. of three experiments performed in triplicate. * p
    Reverse Transcription Polymerase Chain Reaction Rt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription polymerase chain reaction rt pcr kit/product/TaKaRa
    Average 90 stars, based on 136 article reviews
    Price from $9.99 to $1999.99
    reverse transcription polymerase chain reaction rt pcr kit - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    93
    Thermo Fisher reverse transcription polymerase chain reaction rt pcr kits
    Changes in SpSOS1 and <t>SpAHA1</t> mRNAs in S . portulacastrum exposed to salinity. Seedlings were treated with 400, 600 or 800 mM NaCl. The expression of SpSOS1 (A, B) and SpAHA1 (C, D) was analyzed in roots (A, C) and leaves (B, D) at different time intervals (0, 3, 6, 9, 12, 24 and 48 h) under salt treatment using real-time <t>PCR.</t> The expression value at the initial time (0 h) was set to 1, and the expression levels at the other time points under salinity treatment were calculated as the fold of the value at 0 h. Values are expressed as the means±SE (n = 3).
    Reverse Transcription Polymerase Chain Reaction Rt Pcr Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription polymerase chain reaction rt pcr kits/product/Thermo Fisher
    Average 93 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    reverse transcription polymerase chain reaction rt pcr kits - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    92
    Eppendorf AG reverse transcription polymerase chain reaction rt pcr mastercycler
    Changes in SpSOS1 and <t>SpAHA1</t> mRNAs in S . portulacastrum exposed to salinity. Seedlings were treated with 400, 600 or 800 mM NaCl. The expression of SpSOS1 (A, B) and SpAHA1 (C, D) was analyzed in roots (A, C) and leaves (B, D) at different time intervals (0, 3, 6, 9, 12, 24 and 48 h) under salt treatment using real-time <t>PCR.</t> The expression value at the initial time (0 h) was set to 1, and the expression levels at the other time points under salinity treatment were calculated as the fold of the value at 0 h. Values are expressed as the means±SE (n = 3).
    Reverse Transcription Polymerase Chain Reaction Rt Pcr Mastercycler, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription polymerase chain reaction rt pcr mastercycler/product/Eppendorf AG
    Average 92 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    reverse transcription polymerase chain reaction rt pcr mastercycler - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    86
    TaKaRa reverse transcription polymerase chain reaction rt pcr protocol
    ID proteins inhibit E-cadherin expression in breast cells. Notes: ( A ) Western blot analysis of MCF10A cells with stable expression of <t>ID1,</t> ID2, or ID3. ( B ) Real-time <t>PCR</t> analysis of E-cadherin mRNA abundance in cell lines in ( A ). ( C ) Western blot analysis of E-cadherin protein abundance in cell lines in ( A ). ( D ) Assessment of E-cadherin promoter activity, using luciferase reporter assays, in HEK293T cells. The 1.38 kb DNA sequence containing E-cadherin promoter region was cloned upstream of the luciferase gene in a reporter construct (top image). The relative luciferase activities of luciferase reporters with E-cadherin promoter were determined in HEK293T cells, which were cotransfected with the ID1, ID2, ID3, and control vectors. For ( B ) and ( D , bottom image), data represent mean values, with error bars indicating SEM. ** P
    Reverse Transcription Polymerase Chain Reaction Rt Pcr Protocol, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription polymerase chain reaction rt pcr protocol/product/TaKaRa
    Average 86 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    reverse transcription polymerase chain reaction rt pcr protocol - by Bioz Stars, 2020-07
    86/100 stars
      Buy from Supplier

    92
    Promega reverse transcription polymerase chain reaction rt pcr system
    ID proteins inhibit E-cadherin expression in breast cells. Notes: ( A ) Western blot analysis of MCF10A cells with stable expression of <t>ID1,</t> ID2, or ID3. ( B ) Real-time <t>PCR</t> analysis of E-cadherin mRNA abundance in cell lines in ( A ). ( C ) Western blot analysis of E-cadherin protein abundance in cell lines in ( A ). ( D ) Assessment of E-cadherin promoter activity, using luciferase reporter assays, in HEK293T cells. The 1.38 kb DNA sequence containing E-cadherin promoter region was cloned upstream of the luciferase gene in a reporter construct (top image). The relative luciferase activities of luciferase reporters with E-cadherin promoter were determined in HEK293T cells, which were cotransfected with the ID1, ID2, ID3, and control vectors. For ( B ) and ( D , bottom image), data represent mean values, with error bars indicating SEM. ** P
    Reverse Transcription Polymerase Chain Reaction Rt Pcr System, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription polymerase chain reaction rt pcr system/product/Promega
    Average 92 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    reverse transcription polymerase chain reaction rt pcr system - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    91
    Thermo Fisher reverse transcription polymerase chain reaction rt pcr trizol
    ID proteins inhibit E-cadherin expression in breast cells. Notes: ( A ) Western blot analysis of MCF10A cells with stable expression of <t>ID1,</t> ID2, or ID3. ( B ) Real-time <t>PCR</t> analysis of E-cadherin mRNA abundance in cell lines in ( A ). ( C ) Western blot analysis of E-cadherin protein abundance in cell lines in ( A ). ( D ) Assessment of E-cadherin promoter activity, using luciferase reporter assays, in HEK293T cells. The 1.38 kb DNA sequence containing E-cadherin promoter region was cloned upstream of the luciferase gene in a reporter construct (top image). The relative luciferase activities of luciferase reporters with E-cadherin promoter were determined in HEK293T cells, which were cotransfected with the ID1, ID2, ID3, and control vectors. For ( B ) and ( D , bottom image), data represent mean values, with error bars indicating SEM. ** P
    Reverse Transcription Polymerase Chain Reaction Rt Pcr Trizol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription polymerase chain reaction rt pcr trizol/product/Thermo Fisher
    Average 91 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    reverse transcription polymerase chain reaction rt pcr trizol - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    91
    iNtRON Biotechnology conventional reverse transcription polymerase chain reaction rt pcr
    ID proteins inhibit E-cadherin expression in breast cells. Notes: ( A ) Western blot analysis of MCF10A cells with stable expression of <t>ID1,</t> ID2, or ID3. ( B ) Real-time <t>PCR</t> analysis of E-cadherin mRNA abundance in cell lines in ( A ). ( C ) Western blot analysis of E-cadherin protein abundance in cell lines in ( A ). ( D ) Assessment of E-cadherin promoter activity, using luciferase reporter assays, in HEK293T cells. The 1.38 kb DNA sequence containing E-cadherin promoter region was cloned upstream of the luciferase gene in a reporter construct (top image). The relative luciferase activities of luciferase reporters with E-cadherin promoter were determined in HEK293T cells, which were cotransfected with the ID1, ID2, ID3, and control vectors. For ( B ) and ( D , bottom image), data represent mean values, with error bars indicating SEM. ** P
    Conventional Reverse Transcription Polymerase Chain Reaction Rt Pcr, supplied by iNtRON Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/conventional reverse transcription polymerase chain reaction rt pcr/product/iNtRON Biotechnology
    Average 91 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    conventional reverse transcription polymerase chain reaction rt pcr - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    90
    Labnet reverse transcription polymerase chain reaction rt pcr machine
    ID proteins inhibit E-cadherin expression in breast cells. Notes: ( A ) Western blot analysis of MCF10A cells with stable expression of <t>ID1,</t> ID2, or ID3. ( B ) Real-time <t>PCR</t> analysis of E-cadherin mRNA abundance in cell lines in ( A ). ( C ) Western blot analysis of E-cadherin protein abundance in cell lines in ( A ). ( D ) Assessment of E-cadherin promoter activity, using luciferase reporter assays, in HEK293T cells. The 1.38 kb DNA sequence containing E-cadherin promoter region was cloned upstream of the luciferase gene in a reporter construct (top image). The relative luciferase activities of luciferase reporters with E-cadherin promoter were determined in HEK293T cells, which were cotransfected with the ID1, ID2, ID3, and control vectors. For ( B ) and ( D , bottom image), data represent mean values, with error bars indicating SEM. ** P
    Reverse Transcription Polymerase Chain Reaction Rt Pcr Machine, supplied by Labnet, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription polymerase chain reaction rt pcr machine/product/Labnet
    Average 90 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    reverse transcription polymerase chain reaction rt pcr machine - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    91
    Promega reverse transcription polymerase chain reaction rt pcr kit
    ID proteins inhibit E-cadherin expression in breast cells. Notes: ( A ) Western blot analysis of MCF10A cells with stable expression of <t>ID1,</t> ID2, or ID3. ( B ) Real-time <t>PCR</t> analysis of E-cadherin mRNA abundance in cell lines in ( A ). ( C ) Western blot analysis of E-cadherin protein abundance in cell lines in ( A ). ( D ) Assessment of E-cadherin promoter activity, using luciferase reporter assays, in HEK293T cells. The 1.38 kb DNA sequence containing E-cadherin promoter region was cloned upstream of the luciferase gene in a reporter construct (top image). The relative luciferase activities of luciferase reporters with E-cadherin promoter were determined in HEK293T cells, which were cotransfected with the ID1, ID2, ID3, and control vectors. For ( B ) and ( D , bottom image), data represent mean values, with error bars indicating SEM. ** P
    Reverse Transcription Polymerase Chain Reaction Rt Pcr Kit, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription polymerase chain reaction rt pcr kit/product/Promega
    Average 91 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    reverse transcription polymerase chain reaction rt pcr kit - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    92
    tiangen biotech co reverse transcription polymerase chain reaction rt pcr kit
    ID proteins inhibit E-cadherin expression in breast cells. Notes: ( A ) Western blot analysis of MCF10A cells with stable expression of <t>ID1,</t> ID2, or ID3. ( B ) Real-time <t>PCR</t> analysis of E-cadherin mRNA abundance in cell lines in ( A ). ( C ) Western blot analysis of E-cadherin protein abundance in cell lines in ( A ). ( D ) Assessment of E-cadherin promoter activity, using luciferase reporter assays, in HEK293T cells. The 1.38 kb DNA sequence containing E-cadherin promoter region was cloned upstream of the luciferase gene in a reporter construct (top image). The relative luciferase activities of luciferase reporters with E-cadherin promoter were determined in HEK293T cells, which were cotransfected with the ID1, ID2, ID3, and control vectors. For ( B ) and ( D , bottom image), data represent mean values, with error bars indicating SEM. ** P
    Reverse Transcription Polymerase Chain Reaction Rt Pcr Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription polymerase chain reaction rt pcr kit/product/tiangen biotech co
    Average 92 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    reverse transcription polymerase chain reaction rt pcr kit - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Toyobo reverse transcription polymerase chain reaction rt pcr kit
    ID proteins inhibit E-cadherin expression in breast cells. Notes: ( A ) Western blot analysis of MCF10A cells with stable expression of <t>ID1,</t> ID2, or ID3. ( B ) Real-time <t>PCR</t> analysis of E-cadherin mRNA abundance in cell lines in ( A ). ( C ) Western blot analysis of E-cadherin protein abundance in cell lines in ( A ). ( D ) Assessment of E-cadherin promoter activity, using luciferase reporter assays, in HEK293T cells. The 1.38 kb DNA sequence containing E-cadherin promoter region was cloned upstream of the luciferase gene in a reporter construct (top image). The relative luciferase activities of luciferase reporters with E-cadherin promoter were determined in HEK293T cells, which were cotransfected with the ID1, ID2, ID3, and control vectors. For ( B ) and ( D , bottom image), data represent mean values, with error bars indicating SEM. ** P
    Reverse Transcription Polymerase Chain Reaction Rt Pcr Kit, supplied by Toyobo, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription polymerase chain reaction rt pcr kit/product/Toyobo
    Average 92 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    reverse transcription polymerase chain reaction rt pcr kit - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    88
    Thermo Fisher reverse transcription polymerase chain reaction rt pcr semi quantitative rt pcr
    ID proteins inhibit E-cadherin expression in breast cells. Notes: ( A ) Western blot analysis of MCF10A cells with stable expression of <t>ID1,</t> ID2, or ID3. ( B ) Real-time <t>PCR</t> analysis of E-cadherin mRNA abundance in cell lines in ( A ). ( C ) Western blot analysis of E-cadherin protein abundance in cell lines in ( A ). ( D ) Assessment of E-cadherin promoter activity, using luciferase reporter assays, in HEK293T cells. The 1.38 kb DNA sequence containing E-cadherin promoter region was cloned upstream of the luciferase gene in a reporter construct (top image). The relative luciferase activities of luciferase reporters with E-cadherin promoter were determined in HEK293T cells, which were cotransfected with the ID1, ID2, ID3, and control vectors. For ( B ) and ( D , bottom image), data represent mean values, with error bars indicating SEM. ** P
    Reverse Transcription Polymerase Chain Reaction Rt Pcr Semi Quantitative Rt Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription polymerase chain reaction rt pcr semi quantitative rt pcr/product/Thermo Fisher
    Average 88 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    reverse transcription polymerase chain reaction rt pcr semi quantitative rt pcr - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    90
    TaKaRa reverse transcription polymerase chain reaction rt pcr analysis
    <t>Pluripotency</t> of patient-derived iPSCs. (A) Embryoid bodies expressed differentiation markers specific to all three germ layers. To examine the differentiation potential of iPSCs, we evaluated their ability to form embryoid bodies by using floating culture assays. <t>RT-PCR</t> was used to examine the expression of differentiation markers in the embryoid bodies. AFP was used as an endodermal marker, MSX1 as a mesodermal marker, and MAP as an ectodermal marker, with β-actin used as an internal control. (B) Teratoma formation in G-OFiPSCs. Patient-derived iPSCs (G-OF1iPS clones 1 and 6, and G-OF2iPS clones 5 and 8) were injected at a concentration of 1 × 10 6 cells in 20 μl of PBS into 8–10-week-old male CB17 SCID mice. Tumors were collected 9–12 weeks after the injections. Histological examination demonstrated that tissues originating from all three embryonic germ layers were present, including cartilage (mesoderm), gut-like epithelium (endoderm), and neural tube-like structures (ectoderm).
    Reverse Transcription Polymerase Chain Reaction Rt Pcr Analysis, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription polymerase chain reaction rt pcr analysis/product/TaKaRa
    Average 90 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    reverse transcription polymerase chain reaction rt pcr analysis - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    93
    Roche reverse transcription polymerase chain reaction rt pcr kit
    <t>Pluripotency</t> of patient-derived iPSCs. (A) Embryoid bodies expressed differentiation markers specific to all three germ layers. To examine the differentiation potential of iPSCs, we evaluated their ability to form embryoid bodies by using floating culture assays. <t>RT-PCR</t> was used to examine the expression of differentiation markers in the embryoid bodies. AFP was used as an endodermal marker, MSX1 as a mesodermal marker, and MAP as an ectodermal marker, with β-actin used as an internal control. (B) Teratoma formation in G-OFiPSCs. Patient-derived iPSCs (G-OF1iPS clones 1 and 6, and G-OF2iPS clones 5 and 8) were injected at a concentration of 1 × 10 6 cells in 20 μl of PBS into 8–10-week-old male CB17 SCID mice. Tumors were collected 9–12 weeks after the injections. Histological examination demonstrated that tissues originating from all three embryonic germ layers were present, including cartilage (mesoderm), gut-like epithelium (endoderm), and neural tube-like structures (ectoderm).
    Reverse Transcription Polymerase Chain Reaction Rt Pcr Kit, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription polymerase chain reaction rt pcr kit/product/Roche
    Average 93 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    reverse transcription polymerase chain reaction rt pcr kit - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    88
    Thermo Fisher reverse transcription polymerase chain reaction rt pcr primers
    <t>Pluripotency</t> of patient-derived iPSCs. (A) Embryoid bodies expressed differentiation markers specific to all three germ layers. To examine the differentiation potential of iPSCs, we evaluated their ability to form embryoid bodies by using floating culture assays. <t>RT-PCR</t> was used to examine the expression of differentiation markers in the embryoid bodies. AFP was used as an endodermal marker, MSX1 as a mesodermal marker, and MAP as an ectodermal marker, with β-actin used as an internal control. (B) Teratoma formation in G-OFiPSCs. Patient-derived iPSCs (G-OF1iPS clones 1 and 6, and G-OF2iPS clones 5 and 8) were injected at a concentration of 1 × 10 6 cells in 20 μl of PBS into 8–10-week-old male CB17 SCID mice. Tumors were collected 9–12 weeks after the injections. Histological examination demonstrated that tissues originating from all three embryonic germ layers were present, including cartilage (mesoderm), gut-like epithelium (endoderm), and neural tube-like structures (ectoderm).
    Reverse Transcription Polymerase Chain Reaction Rt Pcr Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription polymerase chain reaction rt pcr primers/product/Thermo Fisher
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    reverse transcription polymerase chain reaction rt pcr primers - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    88
    iNtRON Biotechnology reverse transcription polymerase chain reaction rt pcr kits
    <t>Pluripotency</t> of patient-derived iPSCs. (A) Embryoid bodies expressed differentiation markers specific to all three germ layers. To examine the differentiation potential of iPSCs, we evaluated their ability to form embryoid bodies by using floating culture assays. <t>RT-PCR</t> was used to examine the expression of differentiation markers in the embryoid bodies. AFP was used as an endodermal marker, MSX1 as a mesodermal marker, and MAP as an ectodermal marker, with β-actin used as an internal control. (B) Teratoma formation in G-OFiPSCs. Patient-derived iPSCs (G-OF1iPS clones 1 and 6, and G-OF2iPS clones 5 and 8) were injected at a concentration of 1 × 10 6 cells in 20 μl of PBS into 8–10-week-old male CB17 SCID mice. Tumors were collected 9–12 weeks after the injections. Histological examination demonstrated that tissues originating from all three embryonic germ layers were present, including cartilage (mesoderm), gut-like epithelium (endoderm), and neural tube-like structures (ectoderm).
    Reverse Transcription Polymerase Chain Reaction Rt Pcr Kits, supplied by iNtRON Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription polymerase chain reaction rt pcr kits/product/iNtRON Biotechnology
    Average 88 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    reverse transcription polymerase chain reaction rt pcr kits - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    92
    Lonza reverse transcription polymerase chain reaction rt pcr huvecs
    Expression of ENHO (adropin gene) and GPR19 in human vascular cells and the effect of adropin on foam cell formation and inflammatory phenotype in THP1 monocyte-derived macrophages. ( A ) mRNA expression levels of ENHO and GPR19 in THP1 monocytes, their derived macrophages, HASMCs, <t>HUVECs,</t> and HAECs were analyzed by <t>RT-PCR.</t> Glyceraldehyde-3-dehydrogenase ( GAPDH ) served as a loading control. Independent experiments were repeated twice to assure reproducibility. ( B ) THP1 monocytes were incubated for 6 days with the indicated concentrations of adropin, followed by a 19-h incubation with 50 μg/mL oxidized LDL in the presence of 100 μmol/L [ 3 H]oleate. Foam cell formation was determined from reads of the intracellular radioactivity of cholesterol-[ 3 H]oleate ( n = 5). The results from 5 independent experiments are shown in different colors. ( C ) THP1 monocyte-derived macrophages cultured for 6 days were harvested before the addition of oxidized LDL for immunoblot for SRA, ACAT1, NCEH, ABCA1, and β-actin ( n = 5–6). ( D ) THP1 monocytes were incubated for the indicated times with or without adropin (10 ng/mL). Cells were subjected to immunoblot for CD68 (a macrophage differentiation marker), MARCO (an M1 macrophage marker), arginase 1 (an M2 macrophage marker), p-NFκB, PPARγ, or β-actin ( n = 3–4). The graph shows the expressions on day 6. * p
    Reverse Transcription Polymerase Chain Reaction Rt Pcr Huvecs, supplied by Lonza, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription polymerase chain reaction rt pcr huvecs/product/Lonza
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    reverse transcription polymerase chain reaction rt pcr huvecs - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    88
    TaKaRa reverse transcription polymerase chain reaction rt pcr reagents
    Expression of ENHO (adropin gene) and GPR19 in human vascular cells and the effect of adropin on foam cell formation and inflammatory phenotype in THP1 monocyte-derived macrophages. ( A ) mRNA expression levels of ENHO and GPR19 in THP1 monocytes, their derived macrophages, HASMCs, <t>HUVECs,</t> and HAECs were analyzed by <t>RT-PCR.</t> Glyceraldehyde-3-dehydrogenase ( GAPDH ) served as a loading control. Independent experiments were repeated twice to assure reproducibility. ( B ) THP1 monocytes were incubated for 6 days with the indicated concentrations of adropin, followed by a 19-h incubation with 50 μg/mL oxidized LDL in the presence of 100 μmol/L [ 3 H]oleate. Foam cell formation was determined from reads of the intracellular radioactivity of cholesterol-[ 3 H]oleate ( n = 5). The results from 5 independent experiments are shown in different colors. ( C ) THP1 monocyte-derived macrophages cultured for 6 days were harvested before the addition of oxidized LDL for immunoblot for SRA, ACAT1, NCEH, ABCA1, and β-actin ( n = 5–6). ( D ) THP1 monocytes were incubated for the indicated times with or without adropin (10 ng/mL). Cells were subjected to immunoblot for CD68 (a macrophage differentiation marker), MARCO (an M1 macrophage marker), arginase 1 (an M2 macrophage marker), p-NFκB, PPARγ, or β-actin ( n = 3–4). The graph shows the expressions on day 6. * p
    Reverse Transcription Polymerase Chain Reaction Rt Pcr Reagents, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription polymerase chain reaction rt pcr reagents/product/TaKaRa
    Average 88 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    reverse transcription polymerase chain reaction rt pcr reagents - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    92
    Agilent technologies mx3005p reverse transcription polymerase chain reaction rt pcr instrument
    Expression of ENHO (adropin gene) and GPR19 in human vascular cells and the effect of adropin on foam cell formation and inflammatory phenotype in THP1 monocyte-derived macrophages. ( A ) mRNA expression levels of ENHO and GPR19 in THP1 monocytes, their derived macrophages, HASMCs, <t>HUVECs,</t> and HAECs were analyzed by <t>RT-PCR.</t> Glyceraldehyde-3-dehydrogenase ( GAPDH ) served as a loading control. Independent experiments were repeated twice to assure reproducibility. ( B ) THP1 monocytes were incubated for 6 days with the indicated concentrations of adropin, followed by a 19-h incubation with 50 μg/mL oxidized LDL in the presence of 100 μmol/L [ 3 H]oleate. Foam cell formation was determined from reads of the intracellular radioactivity of cholesterol-[ 3 H]oleate ( n = 5). The results from 5 independent experiments are shown in different colors. ( C ) THP1 monocyte-derived macrophages cultured for 6 days were harvested before the addition of oxidized LDL for immunoblot for SRA, ACAT1, NCEH, ABCA1, and β-actin ( n = 5–6). ( D ) THP1 monocytes were incubated for the indicated times with or without adropin (10 ng/mL). Cells were subjected to immunoblot for CD68 (a macrophage differentiation marker), MARCO (an M1 macrophage marker), arginase 1 (an M2 macrophage marker), p-NFκB, PPARγ, or β-actin ( n = 3–4). The graph shows the expressions on day 6. * p
    Mx3005p Reverse Transcription Polymerase Chain Reaction Rt Pcr Instrument, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mx3005p reverse transcription polymerase chain reaction rt pcr instrument/product/Agilent technologies
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    mx3005p reverse transcription polymerase chain reaction rt pcr instrument - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    Changes in BCL-2 family expression caused by rapamycin treatment and FAK down-regulation. REH-empty vector or REH-FAK shRNA cells were cultured with or without rapamycin (100 nM). After 10 h, RNA was extracted from these cells and mRNA expression was detected using quantitative real-time PCR. The expression of pro-apoptosis genes, such as BIK, PUMA, BMF, BAX, and MCL-1S, was higher in the combination treatment group than in the rapamycin-only group. The expression of anti-apoptosis genes, such as BCL-2, was decreased in the combination treatment group compared with the rapamycin-only group. The results represent the mean ± S.D. of three experiments performed in triplicate. * p

    Journal: Journal of Hematology & Oncology

    Article Title: Synergism between the mTOR inhibitor rapamycin and FAK down-regulation in the treatment of acute lymphoblastic leukemia

    doi: 10.1186/s13045-016-0241-x

    Figure Lengend Snippet: Changes in BCL-2 family expression caused by rapamycin treatment and FAK down-regulation. REH-empty vector or REH-FAK shRNA cells were cultured with or without rapamycin (100 nM). After 10 h, RNA was extracted from these cells and mRNA expression was detected using quantitative real-time PCR. The expression of pro-apoptosis genes, such as BIK, PUMA, BMF, BAX, and MCL-1S, was higher in the combination treatment group than in the rapamycin-only group. The expression of anti-apoptosis genes, such as BCL-2, was decreased in the combination treatment group compared with the rapamycin-only group. The results represent the mean ± S.D. of three experiments performed in triplicate. * p

    Article Snippet: Total RNA was extracted using the TRIzol reagent (Invitrogen, USA) according to the manufacturer’s protocols. cDNA was prepared from 1 μg of total RNA using a reverse transcription-polymerase chain reaction (RT-PCR) kit (Takara, Japan) with oligodT according to the manufacturer’s instructions. cDNA samples were then analyzed via quantitative real-time PCR using SYBR Green (Takara, Japan) in an ABI Step One Real-Time PCR machine (Applied Biosystems, Foster City, CA), with 40 cycles of 95 °C for 15 s and 60 °C for 30 s. The efficiency of cDNA synthesis was estimated using hGAPDH as a house-keeping gene.

    Techniques: Expressing, Plasmid Preparation, shRNA, Cell Culture, Real-time Polymerase Chain Reaction

    Changes in SpSOS1 and SpAHA1 mRNAs in S . portulacastrum exposed to salinity. Seedlings were treated with 400, 600 or 800 mM NaCl. The expression of SpSOS1 (A, B) and SpAHA1 (C, D) was analyzed in roots (A, C) and leaves (B, D) at different time intervals (0, 3, 6, 9, 12, 24 and 48 h) under salt treatment using real-time PCR. The expression value at the initial time (0 h) was set to 1, and the expression levels at the other time points under salinity treatment were calculated as the fold of the value at 0 h. Values are expressed as the means±SE (n = 3).

    Journal: PLoS ONE

    Article Title: SpAHA1 and SpSOS1 Coordinate in Transgenic Yeast to Improve Salt Tolerance

    doi: 10.1371/journal.pone.0137447

    Figure Lengend Snippet: Changes in SpSOS1 and SpAHA1 mRNAs in S . portulacastrum exposed to salinity. Seedlings were treated with 400, 600 or 800 mM NaCl. The expression of SpSOS1 (A, B) and SpAHA1 (C, D) was analyzed in roots (A, C) and leaves (B, D) at different time intervals (0, 3, 6, 9, 12, 24 and 48 h) under salt treatment using real-time PCR. The expression value at the initial time (0 h) was set to 1, and the expression levels at the other time points under salinity treatment were calculated as the fold of the value at 0 h. Values are expressed as the means±SE (n = 3).

    Article Snippet: Cloning of SpSOS1 and SpAHA1 Total RNA isolation and cDNA synthesis were performed using a reverse transcription polymerase chain reaction (RT-PCR) kit (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol.

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    ID proteins inhibit E-cadherin expression in breast cells. Notes: ( A ) Western blot analysis of MCF10A cells with stable expression of ID1, ID2, or ID3. ( B ) Real-time PCR analysis of E-cadherin mRNA abundance in cell lines in ( A ). ( C ) Western blot analysis of E-cadherin protein abundance in cell lines in ( A ). ( D ) Assessment of E-cadherin promoter activity, using luciferase reporter assays, in HEK293T cells. The 1.38 kb DNA sequence containing E-cadherin promoter region was cloned upstream of the luciferase gene in a reporter construct (top image). The relative luciferase activities of luciferase reporters with E-cadherin promoter were determined in HEK293T cells, which were cotransfected with the ID1, ID2, ID3, and control vectors. For ( B ) and ( D , bottom image), data represent mean values, with error bars indicating SEM. ** P

    Journal: OncoTargets and therapy

    Article Title: ID2 predicts poor prognosis in breast cancer, especially in triple-negative breast cancer, and inhibits E-cadherin expression

    doi: 10.2147/OTT.S64759

    Figure Lengend Snippet: ID proteins inhibit E-cadherin expression in breast cells. Notes: ( A ) Western blot analysis of MCF10A cells with stable expression of ID1, ID2, or ID3. ( B ) Real-time PCR analysis of E-cadherin mRNA abundance in cell lines in ( A ). ( C ) Western blot analysis of E-cadherin protein abundance in cell lines in ( A ). ( D ) Assessment of E-cadherin promoter activity, using luciferase reporter assays, in HEK293T cells. The 1.38 kb DNA sequence containing E-cadherin promoter region was cloned upstream of the luciferase gene in a reporter construct (top image). The relative luciferase activities of luciferase reporters with E-cadherin promoter were determined in HEK293T cells, which were cotransfected with the ID1, ID2, ID3, and control vectors. For ( B ) and ( D , bottom image), data represent mean values, with error bars indicating SEM. ** P

    Article Snippet: Plasmid constructs The human full-length coding sequences of ID1, ID2, and ID3 were cloned using a standard reverse-transcription polymerase chain reaction (RT-PCR) protocol (Takara Bio Company, Dalian, Japan).

    Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Activity Assay, Luciferase, Sequencing, Clone Assay, Construct

    Pluripotency of patient-derived iPSCs. (A) Embryoid bodies expressed differentiation markers specific to all three germ layers. To examine the differentiation potential of iPSCs, we evaluated their ability to form embryoid bodies by using floating culture assays. RT-PCR was used to examine the expression of differentiation markers in the embryoid bodies. AFP was used as an endodermal marker, MSX1 as a mesodermal marker, and MAP as an ectodermal marker, with β-actin used as an internal control. (B) Teratoma formation in G-OFiPSCs. Patient-derived iPSCs (G-OF1iPS clones 1 and 6, and G-OF2iPS clones 5 and 8) were injected at a concentration of 1 × 10 6 cells in 20 μl of PBS into 8–10-week-old male CB17 SCID mice. Tumors were collected 9–12 weeks after the injections. Histological examination demonstrated that tissues originating from all three embryonic germ layers were present, including cartilage (mesoderm), gut-like epithelium (endoderm), and neural tube-like structures (ectoderm).

    Journal: PLoS ONE

    Article Title: Gorlin syndrome-derived induced pluripotent stem cells are hypersensitive to hedgehog-mediated osteogenic induction

    doi: 10.1371/journal.pone.0186879

    Figure Lengend Snippet: Pluripotency of patient-derived iPSCs. (A) Embryoid bodies expressed differentiation markers specific to all three germ layers. To examine the differentiation potential of iPSCs, we evaluated their ability to form embryoid bodies by using floating culture assays. RT-PCR was used to examine the expression of differentiation markers in the embryoid bodies. AFP was used as an endodermal marker, MSX1 as a mesodermal marker, and MAP as an ectodermal marker, with β-actin used as an internal control. (B) Teratoma formation in G-OFiPSCs. Patient-derived iPSCs (G-OF1iPS clones 1 and 6, and G-OF2iPS clones 5 and 8) were injected at a concentration of 1 × 10 6 cells in 20 μl of PBS into 8–10-week-old male CB17 SCID mice. Tumors were collected 9–12 weeks after the injections. Histological examination demonstrated that tissues originating from all three embryonic germ layers were present, including cartilage (mesoderm), gut-like epithelium (endoderm), and neural tube-like structures (ectoderm).

    Article Snippet: The stemness and pluripotency of the iPSCs were analyzed using reverse transcription polymerase chain reaction (RT-PCR) analysis with ExTaq DNA polymerase (Takara Biotechnology, Shiga, Japan).

    Techniques: Derivative Assay, Reverse Transcription Polymerase Chain Reaction, Expressing, Marker, Clone Assay, Injection, Concentration Assay, Mouse Assay

    Hh-dependent induction of osteogenic marker ALP in patient-derived iPSCs. (A) Control iPSCs (KD iPSCs, 201B7iPS cells, and Nips B2 iPSCs) and patient-derived iPSCs (G-OF1iPS1, and G-OF2iPS5) were cultured in OBM, OBM with FIT (bFGF, IGF-1, and TGF-β1), or OBM with SAG. FIT- and SAG-induced ALP activity significantly increased in patient-derived iPSCs compared with control iPSCs. (B) A sample of each supernatant was transferred to tubes for measurement of ALP activity as the hydrolysis of ρ-nitrophenyl phosphate using a LabAssay ALP kit and protein concentration using a Protein assay rapid kit. G-OF1iPS1 was showed a high ALP activity compared with 201B7,especially cultured in OBM with SAG. (C) KDiPSCs and G-OF1iPS1 cells were cultured in OBM (control), or OBM supplemented with 100 ng/ml BMP2/7 for 24 h. qRT-PCR analysis of RUNX2 was conducted using Premix Ex Taq reagent according to the manufacturer’s instructions. RUNX2 levels were normalized to those of GAPDH. A significant difference was observed in G-OF1iPS1. Especially, BMP2/7-induced RUNX2 expression promoted an approximate 2-fold increase in the number of G-OFiPSCs compared with KDiPSCs. Values represent the mean ± SD. Bonferroni correction for multiple comparisons was applied, * p

    Journal: PLoS ONE

    Article Title: Gorlin syndrome-derived induced pluripotent stem cells are hypersensitive to hedgehog-mediated osteogenic induction

    doi: 10.1371/journal.pone.0186879

    Figure Lengend Snippet: Hh-dependent induction of osteogenic marker ALP in patient-derived iPSCs. (A) Control iPSCs (KD iPSCs, 201B7iPS cells, and Nips B2 iPSCs) and patient-derived iPSCs (G-OF1iPS1, and G-OF2iPS5) were cultured in OBM, OBM with FIT (bFGF, IGF-1, and TGF-β1), or OBM with SAG. FIT- and SAG-induced ALP activity significantly increased in patient-derived iPSCs compared with control iPSCs. (B) A sample of each supernatant was transferred to tubes for measurement of ALP activity as the hydrolysis of ρ-nitrophenyl phosphate using a LabAssay ALP kit and protein concentration using a Protein assay rapid kit. G-OF1iPS1 was showed a high ALP activity compared with 201B7,especially cultured in OBM with SAG. (C) KDiPSCs and G-OF1iPS1 cells were cultured in OBM (control), or OBM supplemented with 100 ng/ml BMP2/7 for 24 h. qRT-PCR analysis of RUNX2 was conducted using Premix Ex Taq reagent according to the manufacturer’s instructions. RUNX2 levels were normalized to those of GAPDH. A significant difference was observed in G-OF1iPS1. Especially, BMP2/7-induced RUNX2 expression promoted an approximate 2-fold increase in the number of G-OFiPSCs compared with KDiPSCs. Values represent the mean ± SD. Bonferroni correction for multiple comparisons was applied, * p

    Article Snippet: The stemness and pluripotency of the iPSCs were analyzed using reverse transcription polymerase chain reaction (RT-PCR) analysis with ExTaq DNA polymerase (Takara Biotechnology, Shiga, Japan).

    Techniques: Marker, ALP Assay, Derivative Assay, Cell Culture, Activity Assay, Protein Concentration, Quantitative RT-PCR, Expressing

    Distinct expression patterns of Hh-associated genes in control and patient-derived iPSCs under osteogenic conditions. We investigated two controls (KDiPS and 201B7) and four Gorlin iPS cases derived from different patients. Because we used single RT 2 Profiler PCR Array plate for each case, we did not performed statistical analysis. (A) Osteogeic induction promoted characteristic changes in the expression levels of the following genes associated with the canonical Hh pathway: GLI1 , GLI2 , GLI3 , and SHH . Compared with the basal conditions, GLI1 and GLI2 expression levels decreased in both controls (KDiPSCs and 201B7) and G-OFiPSCs grown in OBM, whereas GLI3 expression increased only in G-OFiPSCs grown in OBM. Basal expression levels of SHH was significantly lower in G-OFiPSCs compared with controls (KDiPSCs and 201B7). However, OBM enhanced SHH expression levels in G-OFiPSCs but not in control cells. (B) The basal expression levels of many Wnt genes were lower in patient-derived iPSCs than in control iPSCs. With the exception of WNT2 , all of the Wnt genes evaluated were upregulated in G-OFiPSCs grown in OBM and downregulated in control cells (KDiPSCs and 201B7) grown in OBM. (C) Changes in BMP2 , BMP6 , and RUNX2 expression levels were greater in patient-derived iPSCs than in controls (KD iPSCs and 201B7). The ΔΔC t value 10 days after OBM treatment (Post) was determined, and the fold-change relative to pre-induction (Pre) KDiPSCs was calculated for each gene.

    Journal: PLoS ONE

    Article Title: Gorlin syndrome-derived induced pluripotent stem cells are hypersensitive to hedgehog-mediated osteogenic induction

    doi: 10.1371/journal.pone.0186879

    Figure Lengend Snippet: Distinct expression patterns of Hh-associated genes in control and patient-derived iPSCs under osteogenic conditions. We investigated two controls (KDiPS and 201B7) and four Gorlin iPS cases derived from different patients. Because we used single RT 2 Profiler PCR Array plate for each case, we did not performed statistical analysis. (A) Osteogeic induction promoted characteristic changes in the expression levels of the following genes associated with the canonical Hh pathway: GLI1 , GLI2 , GLI3 , and SHH . Compared with the basal conditions, GLI1 and GLI2 expression levels decreased in both controls (KDiPSCs and 201B7) and G-OFiPSCs grown in OBM, whereas GLI3 expression increased only in G-OFiPSCs grown in OBM. Basal expression levels of SHH was significantly lower in G-OFiPSCs compared with controls (KDiPSCs and 201B7). However, OBM enhanced SHH expression levels in G-OFiPSCs but not in control cells. (B) The basal expression levels of many Wnt genes were lower in patient-derived iPSCs than in control iPSCs. With the exception of WNT2 , all of the Wnt genes evaluated were upregulated in G-OFiPSCs grown in OBM and downregulated in control cells (KDiPSCs and 201B7) grown in OBM. (C) Changes in BMP2 , BMP6 , and RUNX2 expression levels were greater in patient-derived iPSCs than in controls (KD iPSCs and 201B7). The ΔΔC t value 10 days after OBM treatment (Post) was determined, and the fold-change relative to pre-induction (Pre) KDiPSCs was calculated for each gene.

    Article Snippet: The stemness and pluripotency of the iPSCs were analyzed using reverse transcription polymerase chain reaction (RT-PCR) analysis with ExTaq DNA polymerase (Takara Biotechnology, Shiga, Japan).

    Techniques: Expressing, Derivative Assay, Polymerase Chain Reaction

    Generation of iPSCs from Gorlin syndrome fibroblasts (G-OFs). (A) We collected fibroblasts from surgically resected unaffected oral skin areas. Typical spindle shape fibroblasts were obtained (a). Fibroblasts (1 × 10 5 /well) were transfected with the Sendai virus vector SeVdp(KOSM)302L. The resulting iPSC colonies were selected 21–25 days after transfection and several clones were subsequently expanded (b). Stable clones (two clones of Patient 1, four clones of Patient 2, two clones of Patient 3, and six clones of Patient 4) exhibited the characteristic morphology of human iPSCs. Representative iPSCs (from passage number 6) are shown (c) (scale bar: 400 μm). (B) We confirmed the infection and silencing of SeVdp using RT-PCR. Cells expressed SeVdp mRNA one day after infection (TF). SeVdp was not detected in any G-OFs (F) or G-OFiPSCs. (C) RT-PCR analysis of embryonic stem cell marker genes in patient-derived iPSCs (G-OF1iPS clones 1 and 6; G-OF2iPS clones 3, 5, 8, and 10; G-OF3iPS clones 25 and 26; and G-OF4iPS clones 3, 8, 12, 15, 18, and 23) and control iPSCs (KDiPSCs). β-actin was used as a loading control. The target genes evaluated included OCT3/4 , SOX2 , NANOG , REX1 , c-MYC , and KLF4 .

    Journal: PLoS ONE

    Article Title: Gorlin syndrome-derived induced pluripotent stem cells are hypersensitive to hedgehog-mediated osteogenic induction

    doi: 10.1371/journal.pone.0186879

    Figure Lengend Snippet: Generation of iPSCs from Gorlin syndrome fibroblasts (G-OFs). (A) We collected fibroblasts from surgically resected unaffected oral skin areas. Typical spindle shape fibroblasts were obtained (a). Fibroblasts (1 × 10 5 /well) were transfected with the Sendai virus vector SeVdp(KOSM)302L. The resulting iPSC colonies were selected 21–25 days after transfection and several clones were subsequently expanded (b). Stable clones (two clones of Patient 1, four clones of Patient 2, two clones of Patient 3, and six clones of Patient 4) exhibited the characteristic morphology of human iPSCs. Representative iPSCs (from passage number 6) are shown (c) (scale bar: 400 μm). (B) We confirmed the infection and silencing of SeVdp using RT-PCR. Cells expressed SeVdp mRNA one day after infection (TF). SeVdp was not detected in any G-OFs (F) or G-OFiPSCs. (C) RT-PCR analysis of embryonic stem cell marker genes in patient-derived iPSCs (G-OF1iPS clones 1 and 6; G-OF2iPS clones 3, 5, 8, and 10; G-OF3iPS clones 25 and 26; and G-OF4iPS clones 3, 8, 12, 15, 18, and 23) and control iPSCs (KDiPSCs). β-actin was used as a loading control. The target genes evaluated included OCT3/4 , SOX2 , NANOG , REX1 , c-MYC , and KLF4 .

    Article Snippet: The stemness and pluripotency of the iPSCs were analyzed using reverse transcription polymerase chain reaction (RT-PCR) analysis with ExTaq DNA polymerase (Takara Biotechnology, Shiga, Japan).

    Techniques: Transfection, Plasmid Preparation, Clone Assay, Infection, Reverse Transcription Polymerase Chain Reaction, Marker, Derivative Assay

    Expression of ENHO (adropin gene) and GPR19 in human vascular cells and the effect of adropin on foam cell formation and inflammatory phenotype in THP1 monocyte-derived macrophages. ( A ) mRNA expression levels of ENHO and GPR19 in THP1 monocytes, their derived macrophages, HASMCs, HUVECs, and HAECs were analyzed by RT-PCR. Glyceraldehyde-3-dehydrogenase ( GAPDH ) served as a loading control. Independent experiments were repeated twice to assure reproducibility. ( B ) THP1 monocytes were incubated for 6 days with the indicated concentrations of adropin, followed by a 19-h incubation with 50 μg/mL oxidized LDL in the presence of 100 μmol/L [ 3 H]oleate. Foam cell formation was determined from reads of the intracellular radioactivity of cholesterol-[ 3 H]oleate ( n = 5). The results from 5 independent experiments are shown in different colors. ( C ) THP1 monocyte-derived macrophages cultured for 6 days were harvested before the addition of oxidized LDL for immunoblot for SRA, ACAT1, NCEH, ABCA1, and β-actin ( n = 5–6). ( D ) THP1 monocytes were incubated for the indicated times with or without adropin (10 ng/mL). Cells were subjected to immunoblot for CD68 (a macrophage differentiation marker), MARCO (an M1 macrophage marker), arginase 1 (an M2 macrophage marker), p-NFκB, PPARγ, or β-actin ( n = 3–4). The graph shows the expressions on day 6. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Adropin Contributes to Anti-Atherosclerosis by Suppressing Monocyte-Endothelial Cell Adhesion and Smooth Muscle Cell Proliferation

    doi: 10.3390/ijms19051293

    Figure Lengend Snippet: Expression of ENHO (adropin gene) and GPR19 in human vascular cells and the effect of adropin on foam cell formation and inflammatory phenotype in THP1 monocyte-derived macrophages. ( A ) mRNA expression levels of ENHO and GPR19 in THP1 monocytes, their derived macrophages, HASMCs, HUVECs, and HAECs were analyzed by RT-PCR. Glyceraldehyde-3-dehydrogenase ( GAPDH ) served as a loading control. Independent experiments were repeated twice to assure reproducibility. ( B ) THP1 monocytes were incubated for 6 days with the indicated concentrations of adropin, followed by a 19-h incubation with 50 μg/mL oxidized LDL in the presence of 100 μmol/L [ 3 H]oleate. Foam cell formation was determined from reads of the intracellular radioactivity of cholesterol-[ 3 H]oleate ( n = 5). The results from 5 independent experiments are shown in different colors. ( C ) THP1 monocyte-derived macrophages cultured for 6 days were harvested before the addition of oxidized LDL for immunoblot for SRA, ACAT1, NCEH, ABCA1, and β-actin ( n = 5–6). ( D ) THP1 monocytes were incubated for the indicated times with or without adropin (10 ng/mL). Cells were subjected to immunoblot for CD68 (a macrophage differentiation marker), MARCO (an M1 macrophage marker), arginase 1 (an M2 macrophage marker), p-NFκB, PPARγ, or β-actin ( n = 3–4). The graph shows the expressions on day 6. * p

    Article Snippet: Reverse Transcription Polymerase Chain Reaction (RT-PCR) HUVECs (Lonza, Walkersville, MD, USA) were incubated at 37 °C in 5% CO2 for 30 min with the indicated concentrations of adropin in EGM-2 (Lonza).

    Techniques: Expressing, Derivative Assay, Reverse Transcription Polymerase Chain Reaction, Incubation, Radioactivity, Cell Culture, Marker

    Effects of adropin on inflammatory response and monocyte adhesion in HUVECs. ( A ) mRNA expression of ICAM1 , VCAM1 , and SELE (selectin E gene) was analyzed by RT-PCR. HUVECs were pre-treated with adropin (0, 10, 100 ng/mL) for 30 min and then incubated with adropin (0, 10, 100 ng/mL) + TNFα (0, 10 ng/mL) for 4 h. Representative images are shown; the graph on the right side indicates densitometry data following normalization relative to GAPDH ( n = 3–4). ( B – D ) HUVECs treated as described above were harvested and subjected to immunoblot to evaluate ICAM1, VCAM1, and selectin E protein expression ( n = 4–5). Upper panels show representative immunoblots with densitometry data after normalization relative to β-actin shown beneath. ( E ) Confluent HUVECs were incubated in 0.5% fetal bovine serum (FBS)-EGM-2 for 16 h, and then pre-treated for 30 min with the indicated concentrations of adropin, followed by a 4-h incubation in the presence or absence of TNFα (10 ng/mL). Subsequently, calcein red-orange-labeled THP1 monocytes were plated on the HUVEC monolayer and incubated for 1 h. After washing, the adherent cells were observed by fluorescence microscopy ( n = 4). Scale bar = 100 μm. Baseline (1 fold) = 66318.5 ± 4599.2 pixels. ( B – E ) * p

    Journal: International Journal of Molecular Sciences

    Article Title: Adropin Contributes to Anti-Atherosclerosis by Suppressing Monocyte-Endothelial Cell Adhesion and Smooth Muscle Cell Proliferation

    doi: 10.3390/ijms19051293

    Figure Lengend Snippet: Effects of adropin on inflammatory response and monocyte adhesion in HUVECs. ( A ) mRNA expression of ICAM1 , VCAM1 , and SELE (selectin E gene) was analyzed by RT-PCR. HUVECs were pre-treated with adropin (0, 10, 100 ng/mL) for 30 min and then incubated with adropin (0, 10, 100 ng/mL) + TNFα (0, 10 ng/mL) for 4 h. Representative images are shown; the graph on the right side indicates densitometry data following normalization relative to GAPDH ( n = 3–4). ( B – D ) HUVECs treated as described above were harvested and subjected to immunoblot to evaluate ICAM1, VCAM1, and selectin E protein expression ( n = 4–5). Upper panels show representative immunoblots with densitometry data after normalization relative to β-actin shown beneath. ( E ) Confluent HUVECs were incubated in 0.5% fetal bovine serum (FBS)-EGM-2 for 16 h, and then pre-treated for 30 min with the indicated concentrations of adropin, followed by a 4-h incubation in the presence or absence of TNFα (10 ng/mL). Subsequently, calcein red-orange-labeled THP1 monocytes were plated on the HUVEC monolayer and incubated for 1 h. After washing, the adherent cells were observed by fluorescence microscopy ( n = 4). Scale bar = 100 μm. Baseline (1 fold) = 66318.5 ± 4599.2 pixels. ( B – E ) * p

    Article Snippet: Reverse Transcription Polymerase Chain Reaction (RT-PCR) HUVECs (Lonza, Walkersville, MD, USA) were incubated at 37 °C in 5% CO2 for 30 min with the indicated concentrations of adropin in EGM-2 (Lonza).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Incubation, Western Blot, Labeling, Fluorescence, Microscopy