rsai restriction endonucleases Search Results


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  • 99
    New England Biolabs restriction endonuclease rsai
    Restriction Endonuclease Rsai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher rsai restriction endonucleases
    1% agarose gel loaded with B. anthracis <t>DNA</t> before and after amplification using REPLI-g genomic DNA amplification kit and <t>RsaI</t> digestion. Lanes 1 and 6, : 1 kb molecular weight markers, lane 2: Hind III molecular weight marker (23130, 9416, 6557, 4361, 2322, 2027, and 546 bp), lane 3, : 5 μ L of 20 ng/ μ L B. anthracis Ba 179 genomic DNA, lane 4, : 5 μ L of REPLI-g amplified DNA (355 ng/ μ L), and lane 5: RsaI digested DNA.
    Rsai Restriction Endonucleases, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Boehringer Mannheim restriction endonuclease rsai
    1% agarose gel loaded with B. anthracis <t>DNA</t> before and after amplification using REPLI-g genomic DNA amplification kit and <t>RsaI</t> digestion. Lanes 1 and 6, : 1 kb molecular weight markers, lane 2: Hind III molecular weight marker (23130, 9416, 6557, 4361, 2322, 2027, and 546 bp), lane 3, : 5 μ L of 20 ng/ μ L B. anthracis Ba 179 genomic DNA, lane 4, : 5 μ L of REPLI-g amplified DNA (355 ng/ μ L), and lane 5: RsaI digested DNA.
    Restriction Endonuclease Rsai, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonuclease rsai/product/Boehringer Mannheim
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    99
    New England Biolabs rsai restriction endonucleases
    Selected areas of DNA methylation in control or arsenic-exposed RWPE-1 cells at 4 weeks of exposure using arbitrarily primed PCR after restriction enzyme digestions. Shown are details of a representative gel with an area of distinct DNA hypomethylation in arsenic-treated DNA ( left panels, two-headed arrows ) and densitometric analysis ( n = 3) of this band ( right panel ). See methods for details. An asterisk indicates a significant difference ( p ≤ 0.05) from control. Unmethylated DNA is not protected from digestion by methylation-sensitive <t>HpaII</t> restriction enzyme resulting in a loss of DNA for PCR amplification. Digestion with <t>RsaI</t> + MspI would also result in no PCR amplification regardless of methylation status. Digestion with RsaI alone and RsaI + MspI were used as controls to determine whether there was differential methylation.
    Rsai Restriction Endonucleases, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 300 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Roche restriction enzyme rsai
    Selected areas of DNA methylation in control or arsenic-exposed RWPE-1 cells at 4 weeks of exposure using arbitrarily primed PCR after restriction enzyme digestions. Shown are details of a representative gel with an area of distinct DNA hypomethylation in arsenic-treated DNA ( left panels, two-headed arrows ) and densitometric analysis ( n = 3) of this band ( right panel ). See methods for details. An asterisk indicates a significant difference ( p ≤ 0.05) from control. Unmethylated DNA is not protected from digestion by methylation-sensitive <t>HpaII</t> restriction enzyme resulting in a loss of DNA for PCR amplification. Digestion with <t>RsaI</t> + MspI would also result in no PCR amplification regardless of methylation status. Digestion with RsaI alone and RsaI + MspI were used as controls to determine whether there was differential methylation.
    Restriction Enzyme Rsai, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore rhodopseudomonas sphaeroides
    Selected areas of DNA methylation in control or arsenic-exposed RWPE-1 cells at 4 weeks of exposure using arbitrarily primed PCR after restriction enzyme digestions. Shown are details of a representative gel with an area of distinct DNA hypomethylation in arsenic-treated DNA ( left panels, two-headed arrows ) and densitometric analysis ( n = 3) of this band ( right panel ). See methods for details. An asterisk indicates a significant difference ( p ≤ 0.05) from control. Unmethylated DNA is not protected from digestion by methylation-sensitive <t>HpaII</t> restriction enzyme resulting in a loss of DNA for PCR amplification. Digestion with <t>RsaI</t> + MspI would also result in no PCR amplification regardless of methylation status. Digestion with RsaI alone and RsaI + MspI were used as controls to determine whether there was differential methylation.
    Rhodopseudomonas Sphaeroides, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    1% agarose gel loaded with B. anthracis DNA before and after amplification using REPLI-g genomic DNA amplification kit and RsaI digestion. Lanes 1 and 6, : 1 kb molecular weight markers, lane 2: Hind III molecular weight marker (23130, 9416, 6557, 4361, 2322, 2027, and 546 bp), lane 3, : 5 μ L of 20 ng/ μ L B. anthracis Ba 179 genomic DNA, lane 4, : 5 μ L of REPLI-g amplified DNA (355 ng/ μ L), and lane 5: RsaI digested DNA.

    Journal: Journal of Pathogens

    Article Title: A New Generation Microarray for the Simultaneous Detection and Identification of Yersinia pestis and Bacillus anthracis in Food

    doi: 10.1155/2012/627036

    Figure Lengend Snippet: 1% agarose gel loaded with B. anthracis DNA before and after amplification using REPLI-g genomic DNA amplification kit and RsaI digestion. Lanes 1 and 6, : 1 kb molecular weight markers, lane 2: Hind III molecular weight marker (23130, 9416, 6557, 4361, 2322, 2027, and 546 bp), lane 3, : 5 μ L of 20 ng/ μ L B. anthracis Ba 179 genomic DNA, lane 4, : 5 μ L of REPLI-g amplified DNA (355 ng/ μ L), and lane 5: RsaI digested DNA.

    Article Snippet: For labeling, 2 μ g of amplified DNA was digested with RsaI (Life Technologies, Carlsbad, CA, USA) at 37°C for 4 hours and subsequently labeled with Alexa Fluor 555 and/or 647 florescent dyes using BioPrime Plus Array CGH Genomic Labeling Kit (Life Technologies, Carlsbad, CA, USA) following the manufacturer's instructions.

    Techniques: Agarose Gel Electrophoresis, Amplification, Molecular Weight, Marker

    Selected areas of DNA methylation in control or arsenic-exposed RWPE-1 cells at 4 weeks of exposure using arbitrarily primed PCR after restriction enzyme digestions. Shown are details of a representative gel with an area of distinct DNA hypomethylation in arsenic-treated DNA ( left panels, two-headed arrows ) and densitometric analysis ( n = 3) of this band ( right panel ). See methods for details. An asterisk indicates a significant difference ( p ≤ 0.05) from control. Unmethylated DNA is not protected from digestion by methylation-sensitive HpaII restriction enzyme resulting in a loss of DNA for PCR amplification. Digestion with RsaI + MspI would also result in no PCR amplification regardless of methylation status. Digestion with RsaI alone and RsaI + MspI were used as controls to determine whether there was differential methylation.

    Journal: The Journal of Biological Chemistry

    Article Title: Interplay between Cellular Methyl Metabolism and Adaptive Efflux during Oncogenic Transformation from Chronic Arsenic Exposure in Human Cells *Interplay between Cellular Methyl Metabolism and Adaptive Efflux during Oncogenic Transformation from Chronic Arsenic Exposure in Human Cells * S⃞

    doi: 10.1074/jbc.M802942200

    Figure Lengend Snippet: Selected areas of DNA methylation in control or arsenic-exposed RWPE-1 cells at 4 weeks of exposure using arbitrarily primed PCR after restriction enzyme digestions. Shown are details of a representative gel with an area of distinct DNA hypomethylation in arsenic-treated DNA ( left panels, two-headed arrows ) and densitometric analysis ( n = 3) of this band ( right panel ). See methods for details. An asterisk indicates a significant difference ( p ≤ 0.05) from control. Unmethylated DNA is not protected from digestion by methylation-sensitive HpaII restriction enzyme resulting in a loss of DNA for PCR amplification. Digestion with RsaI + MspI would also result in no PCR amplification regardless of methylation status. Digestion with RsaI alone and RsaI + MspI were used as controls to determine whether there was differential methylation.

    Article Snippet: Restriction Enzyme Digestions of Genomic DNA —One μg of DNA from control and arsenic-treated cells at 4 weeks of arsenic exposure was separately digested with either 10 units of RsaI, 10 units each of RsaI and the methylation-sensitive restriction enzyme HpaII, or 10 units each of RsaI and MspI (New England BioLabs, Ipswich, MA) at 37 °C for 16 h. This was followed by heat inactivation of the enzymes at 65 °C for 20 min.

    Techniques: DNA Methylation Assay, Polymerase Chain Reaction, Methylation, Amplification

    Schematic of cDNA library preparation. PolyA-selected mRNA (in red) is reverse transcribed using a polyT primer tailed with a universal primer (A). See Table S3 for primer sequences. MMLV reverse transcriptase adds cytosines to the 3′ end of the 1st strand cDNA (in black), allowing for template switching and addition of the 3′ universal primer (B). PCR amplification of the library is followed by RsaI and AluI enzymatic digestion of cDNAs (C), followed by the standard Illumina library preparation steps of end-repair, a single adenine addition, Y-adapter ligation (D), PCR enrichment, and size selection (mock gel shown in E with yellow box indicating area of gel removed for DNA extraction), prior to flowcell generation and sequencing.

    Journal: PLoS ONE

    Article Title: Gene Expression Analysis of Zebrafish Melanocytes, Iridophores, and Retinal Pigmented Epithelium Reveals Indicators of Biological Function and Developmental Origin

    doi: 10.1371/journal.pone.0067801

    Figure Lengend Snippet: Schematic of cDNA library preparation. PolyA-selected mRNA (in red) is reverse transcribed using a polyT primer tailed with a universal primer (A). See Table S3 for primer sequences. MMLV reverse transcriptase adds cytosines to the 3′ end of the 1st strand cDNA (in black), allowing for template switching and addition of the 3′ universal primer (B). PCR amplification of the library is followed by RsaI and AluI enzymatic digestion of cDNAs (C), followed by the standard Illumina library preparation steps of end-repair, a single adenine addition, Y-adapter ligation (D), PCR enrichment, and size selection (mock gel shown in E with yellow box indicating area of gel removed for DNA extraction), prior to flowcell generation and sequencing.

    Article Snippet: Following PCR amplification using the high fidelity polymerase LA Taq (TaKaRa, PCR cycle: 95C for 1 minute, followed by 20 cycles of 98C for 25 seconds, 60C for 1 minute, 68C for 20 minutes), cDNA was digested with AluI and RsaI restriction enzymes (NEB).

    Techniques: cDNA Library Assay, Polymerase Chain Reaction, Amplification, Ligation, Selection, DNA Extraction, Sequencing

    Klenow treatment prior to Bal31 incubation preserves the telomeric signal. Bal31 degradation of 10 μg of RsaI/HinfI-digested genomic DNA from VA-13 cells resulted in a faint signal after (TTAGGG) 4 hybridization. A Klenow fill-in reaction prior

    Journal: DNA and cell biology

    Article Title: Detection of circular telomeric DNA without 2-D gel electrophoresis

    doi: 10.1089/dna.2008.0741

    Figure Lengend Snippet: Klenow treatment prior to Bal31 incubation preserves the telomeric signal. Bal31 degradation of 10 μg of RsaI/HinfI-digested genomic DNA from VA-13 cells resulted in a faint signal after (TTAGGG) 4 hybridization. A Klenow fill-in reaction prior

    Article Snippet: For telomere length analysis 2 μg of genomic DNA was digested over night with the enzymes RsaI and HinfI (1x NEB 2, total volume 25μl; New England Biolabs, Beverly, MA, USA) at 37°C.

    Techniques: Incubation, Hybridization

    Klenwow/Bal31 treatment does not generate a product from linear telomeric DNA. After digesting 40μg of genomic DNA with RsaI and HinfI a biotin-labeled C-rich oligo was annealed to the 3′single-stranded overhang. Pulling down oligo-bound

    Journal: DNA and cell biology

    Article Title: Detection of circular telomeric DNA without 2-D gel electrophoresis

    doi: 10.1089/dna.2008.0741

    Figure Lengend Snippet: Klenwow/Bal31 treatment does not generate a product from linear telomeric DNA. After digesting 40μg of genomic DNA with RsaI and HinfI a biotin-labeled C-rich oligo was annealed to the 3′single-stranded overhang. Pulling down oligo-bound

    Article Snippet: For telomere length analysis 2 μg of genomic DNA was digested over night with the enzymes RsaI and HinfI (1x NEB 2, total volume 25μl; New England Biolabs, Beverly, MA, USA) at 37°C.

    Techniques: Labeling

    The Klenow/Bal31 treatment of ALT cell DNA generates molecules that run as a single arc in 2D gel electrophoresis. 20 μg of RsaI/HinfI-digested genomic DNA from telomerase-positive SW39 (upper part of the figure) and VA13 ALT cells (lower part

    Journal: DNA and cell biology

    Article Title: Detection of circular telomeric DNA without 2-D gel electrophoresis

    doi: 10.1089/dna.2008.0741

    Figure Lengend Snippet: The Klenow/Bal31 treatment of ALT cell DNA generates molecules that run as a single arc in 2D gel electrophoresis. 20 μg of RsaI/HinfI-digested genomic DNA from telomerase-positive SW39 (upper part of the figure) and VA13 ALT cells (lower part

    Article Snippet: For telomere length analysis 2 μg of genomic DNA was digested over night with the enzymes RsaI and HinfI (1x NEB 2, total volume 25μl; New England Biolabs, Beverly, MA, USA) at 37°C.

    Techniques: Two-Dimensional Gel Electrophoresis, Electrophoresis

    DNA-PKcs-deficient MEFs exhibit similar telomere lengths and telomerase activities when compared with wild-type MEFs. ( A ) Southern analysis of MEFs. Early passage MEFs from independently isolated littermates were prepared from wild-type (lanes 7 and 8), DNA-PKcs+/− (lanes 1, 2, 5, and 6), and DNA-PKcs−/− (lanes 3 and 4). Gel plugs containing genomic DNAs were digested with Rsa I and Hin fI (odd number lanes) or undigested (even number lanes), fractionated by pulse-field gel electrophoresis, and hybridized with the telomeric specific [TTAGGG] 3 probe. The approximate sizes of the products (kb) are indicated based on molecular weight markers. The Southern hybridization signal observed with the [TTAGGG] 3 probe under these conditions was sensitive to BAL-31 exonuclease digestion, suggesting that this is telomeric DNA (data not shown). ( B ) PCR analysis for genotyping. Using the specific primer pairs (see Materials and Methods ), wild-type and targeted alleles were amplified as products of 450 bp and 360 bp, respectively. Lanes 2 and 4 show the DNA-PKcs+/− pattern, lane 3 shows the DNA-PKcs−/− pattern, and lane 5 shows the wild-type pattern. Lane 1 contains a size marker. ( C ) Telomerase activity in DNA-PKcs-deficient MEFs. TRAP assay was performed after 30 PCR cycles on cell extracts (10, 10 2 , and 10 3 cells) prepared from DNA-PKcs−/− (lanes 1–3), DNA-PKcs+/− (lanes 4–6), and wild-type (lanes 7–9) MEFs. In lanes 10–12, a serial dilution of HeLa cell lysate was run as a positive control for quantitating relative telomerase activity levels. Lane 13 contains a negative control without cell lysate. IC denotes a standard internal control for PCR efficiency.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: DNA-PKcs is critical for telomere capping

    doi: 10.1073/pnas.261574698

    Figure Lengend Snippet: DNA-PKcs-deficient MEFs exhibit similar telomere lengths and telomerase activities when compared with wild-type MEFs. ( A ) Southern analysis of MEFs. Early passage MEFs from independently isolated littermates were prepared from wild-type (lanes 7 and 8), DNA-PKcs+/− (lanes 1, 2, 5, and 6), and DNA-PKcs−/− (lanes 3 and 4). Gel plugs containing genomic DNAs were digested with Rsa I and Hin fI (odd number lanes) or undigested (even number lanes), fractionated by pulse-field gel electrophoresis, and hybridized with the telomeric specific [TTAGGG] 3 probe. The approximate sizes of the products (kb) are indicated based on molecular weight markers. The Southern hybridization signal observed with the [TTAGGG] 3 probe under these conditions was sensitive to BAL-31 exonuclease digestion, suggesting that this is telomeric DNA (data not shown). ( B ) PCR analysis for genotyping. Using the specific primer pairs (see Materials and Methods ), wild-type and targeted alleles were amplified as products of 450 bp and 360 bp, respectively. Lanes 2 and 4 show the DNA-PKcs+/− pattern, lane 3 shows the DNA-PKcs−/− pattern, and lane 5 shows the wild-type pattern. Lane 1 contains a size marker. ( C ) Telomerase activity in DNA-PKcs-deficient MEFs. TRAP assay was performed after 30 PCR cycles on cell extracts (10, 10 2 , and 10 3 cells) prepared from DNA-PKcs−/− (lanes 1–3), DNA-PKcs+/− (lanes 4–6), and wild-type (lanes 7–9) MEFs. In lanes 10–12, a serial dilution of HeLa cell lysate was run as a positive control for quantitating relative telomerase activity levels. Lane 13 contains a negative control without cell lysate. IC denotes a standard internal control for PCR efficiency.

    Article Snippet: DNA was digested with Hin fI and Rsa I (New England Biolabs) and electrophoresed on a 1% TBE agarose gel at 14°C, using a CHEF DR-II pulsed-field apparatus (Bio-Rad).

    Techniques: Isolation, Nucleic Acid Electrophoresis, Molecular Weight, Hybridization, Polymerase Chain Reaction, Amplification, Marker, Activity Assay, TRAP Assay, Serial Dilution, Positive Control, Negative Control