rsa i Promega Search Results


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  • 95
    Promega rsa i
    Diagrammatic representation of the TMDH process. (A) A library of transposon mutants is obtained using a custom transposon with outward-facing T7 and SP6 promoters. Genomic <t>DNA</t> is extracted from the library and digested using a restriction endonuclease ( Rsa I). Labelled RNA run-offs are obtained from the T7 and SP6 promoters by in vitro transcription. (B) The labelled RNA run-offs are hybridised to genome-wide tiling microarrays. By examining the distribution of microarray signals between <t>Rsa</t> I restriction sites (vertical black lines) it is possible to infer the location of the transposon (green triangle). Comparison of the library grown in vitro (input) with the library obtained after passage through a mouse (output) allows attenuating mutants to be identified, since they give lower signals in the output.
    Rsa I, supplied by Promega, used in various techniques. Bioz Stars score: 95/100, based on 484 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rsa i/product/Promega
    Average 95 stars, based on 484 article reviews
    Price from $9.99 to $1999.99
    rsa i - by Bioz Stars, 2020-02
    95/100 stars
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    79
    Promega enzyme rsa i
    Diagrammatic representation of the TMDH process. (A) A library of transposon mutants is obtained using a custom transposon with outward-facing T7 and SP6 promoters. Genomic <t>DNA</t> is extracted from the library and digested using a restriction endonuclease ( Rsa I). Labelled RNA run-offs are obtained from the T7 and SP6 promoters by in vitro transcription. (B) The labelled RNA run-offs are hybridised to genome-wide tiling microarrays. By examining the distribution of microarray signals between <t>Rsa</t> I restriction sites (vertical black lines) it is possible to infer the location of the transposon (green triangle). Comparison of the library grown in vitro (input) with the library obtained after passage through a mouse (output) allows attenuating mutants to be identified, since they give lower signals in the output.
    Enzyme Rsa I, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzyme rsa i/product/Promega
    Average 79 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    enzyme rsa i - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    78
    Promega rsa i restriction enzyme
    Diagrammatic representation of the TMDH process. (A) A library of transposon mutants is obtained using a custom transposon with outward-facing T7 and SP6 promoters. Genomic <t>DNA</t> is extracted from the library and digested using a restriction endonuclease ( Rsa I). Labelled RNA run-offs are obtained from the T7 and SP6 promoters by in vitro transcription. (B) The labelled RNA run-offs are hybridised to genome-wide tiling microarrays. By examining the distribution of microarray signals between <t>Rsa</t> I restriction sites (vertical black lines) it is possible to infer the location of the transposon (green triangle). Comparison of the library grown in vitro (input) with the library obtained after passage through a mouse (output) allows attenuating mutants to be identified, since they give lower signals in the output.
    Rsa I Restriction Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rsa i restriction enzyme/product/Promega
    Average 78 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    rsa i restriction enzyme - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    81
    Promega rsa i restriction endonucleases
    Diagrammatic representation of the TMDH process. (A) A library of transposon mutants is obtained using a custom transposon with outward-facing T7 and SP6 promoters. Genomic <t>DNA</t> is extracted from the library and digested using a restriction endonuclease ( Rsa I). Labelled RNA run-offs are obtained from the T7 and SP6 promoters by in vitro transcription. (B) The labelled RNA run-offs are hybridised to genome-wide tiling microarrays. By examining the distribution of microarray signals between <t>Rsa</t> I restriction sites (vertical black lines) it is possible to infer the location of the transposon (green triangle). Comparison of the library grown in vitro (input) with the library obtained after passage through a mouse (output) allows attenuating mutants to be identified, since they give lower signals in the output.
    Rsa I Restriction Endonucleases, supplied by Promega, used in various techniques. Bioz Stars score: 81/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rsa i restriction endonucleases/product/Promega
    Average 81 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    rsa i restriction endonucleases - by Bioz Stars, 2020-02
    81/100 stars
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    78
    Promega rsa i restriction enzymes
    Diagrammatic representation of the TMDH process. (A) A library of transposon mutants is obtained using a custom transposon with outward-facing T7 and SP6 promoters. Genomic <t>DNA</t> is extracted from the library and digested using a restriction endonuclease ( Rsa I). Labelled RNA run-offs are obtained from the T7 and SP6 promoters by in vitro transcription. (B) The labelled RNA run-offs are hybridised to genome-wide tiling microarrays. By examining the distribution of microarray signals between <t>Rsa</t> I restriction sites (vertical black lines) it is possible to infer the location of the transposon (green triangle). Comparison of the library grown in vitro (input) with the library obtained after passage through a mouse (output) allows attenuating mutants to be identified, since they give lower signals in the output.
    Rsa I Restriction Enzymes, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rsa i restriction enzymes/product/Promega
    Average 78 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    rsa i restriction enzymes - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    77
    Promega rsa i digestion
    (A) ITS-PCR patterns of Leuconostoc species. (B) <t>Rsa</t> I digestion of the ITS-PCR fragments of Leuconostoc species. Lane 1, W. paramesenteroides ; lane 2, L. citreum ; lane 3, L. lactis ; lane 4, L. mesenteroides subsp. mesenteroides ; lane 5, L. mesenteroides subsp. cremoris ; lane 6, L. mesenteroides subsp. dextranicum ; lane 7, L. amelibiosum ; lane 8, L. fallax ; lanes M, molecular weight markers (100-bp DNA ladder).
    Rsa I Digestion, supplied by Promega, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rsa i digestion/product/Promega
    Average 77 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    rsa i digestion - by Bioz Stars, 2020-02
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    78
    Promega hinf i rsa i
    (A) ITS-PCR patterns of Leuconostoc species. (B) <t>Rsa</t> I digestion of the ITS-PCR fragments of Leuconostoc species. Lane 1, W. paramesenteroides ; lane 2, L. citreum ; lane 3, L. lactis ; lane 4, L. mesenteroides subsp. mesenteroides ; lane 5, L. mesenteroides subsp. cremoris ; lane 6, L. mesenteroides subsp. dextranicum ; lane 7, L. amelibiosum ; lane 8, L. fallax ; lanes M, molecular weight markers (100-bp DNA ladder).
    Hinf I Rsa I, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hinf i rsa i/product/Promega
    Average 78 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    hinf i rsa i - by Bioz Stars, 2020-02
    78/100 stars
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    Image Search Results


    Diagrammatic representation of the TMDH process. (A) A library of transposon mutants is obtained using a custom transposon with outward-facing T7 and SP6 promoters. Genomic DNA is extracted from the library and digested using a restriction endonuclease ( Rsa I). Labelled RNA run-offs are obtained from the T7 and SP6 promoters by in vitro transcription. (B) The labelled RNA run-offs are hybridised to genome-wide tiling microarrays. By examining the distribution of microarray signals between Rsa I restriction sites (vertical black lines) it is possible to infer the location of the transposon (green triangle). Comparison of the library grown in vitro (input) with the library obtained after passage through a mouse (output) allows attenuating mutants to be identified, since they give lower signals in the output.

    Journal: PLoS Pathogens

    Article Title: Comprehensive Identification of Salmonella enterica Serovar Typhimurium Genes Required for Infection of BALB/c Mice

    doi: 10.1371/journal.ppat.1000529

    Figure Lengend Snippet: Diagrammatic representation of the TMDH process. (A) A library of transposon mutants is obtained using a custom transposon with outward-facing T7 and SP6 promoters. Genomic DNA is extracted from the library and digested using a restriction endonuclease ( Rsa I). Labelled RNA run-offs are obtained from the T7 and SP6 promoters by in vitro transcription. (B) The labelled RNA run-offs are hybridised to genome-wide tiling microarrays. By examining the distribution of microarray signals between Rsa I restriction sites (vertical black lines) it is possible to infer the location of the transposon (green triangle). Comparison of the library grown in vitro (input) with the library obtained after passage through a mouse (output) allows attenuating mutants to be identified, since they give lower signals in the output.

    Article Snippet: Restriction digests and in vitro transcription 10 µg of genomic DNA from each TMDH input and output pool, and from the untransposed control DNA, was digested using Rsa I (Promega) overnight at 37°C.

    Techniques: In Vitro, Genome Wide, Microarray

    (A) ITS-PCR patterns of Leuconostoc species. (B) Rsa I digestion of the ITS-PCR fragments of Leuconostoc species. Lane 1, W. paramesenteroides ; lane 2, L. citreum ; lane 3, L. lactis ; lane 4, L. mesenteroides subsp. mesenteroides ; lane 5, L. mesenteroides subsp. cremoris ; lane 6, L. mesenteroides subsp. dextranicum ; lane 7, L. amelibiosum ; lane 8, L. fallax ; lanes M, molecular weight markers (100-bp DNA ladder).

    Journal: Applied and Environmental Microbiology

    Article Title: Identification and Characterization of Leuconostoc fallax Strains Isolated from an Industrial Sauerkraut Fermentation †

    doi: 10.1128/AEM.68.6.2877-2884.2002

    Figure Lengend Snippet: (A) ITS-PCR patterns of Leuconostoc species. (B) Rsa I digestion of the ITS-PCR fragments of Leuconostoc species. Lane 1, W. paramesenteroides ; lane 2, L. citreum ; lane 3, L. lactis ; lane 4, L. mesenteroides subsp. mesenteroides ; lane 5, L. mesenteroides subsp. cremoris ; lane 6, L. mesenteroides subsp. dextranicum ; lane 7, L. amelibiosum ; lane 8, L. fallax ; lanes M, molecular weight markers (100-bp DNA ladder).

    Article Snippet: The fragments obtained were subjected to Rsa I digestion by following the manufacturer's recommendations (Promega).

    Techniques: Polymerase Chain Reaction, Molecular Weight