rsa i Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs rsa i
    F36VFGFR1 supports the survival of long-term repopulating HSCs during growth factor deprivation. Marrow cells (5 × 10 5 ) transduced with the F36VFGFR1 vector were transplanted into lethally irradiated recipients either immediately following transduction (A) or after 1 (B) or 5 days (C) of culture in the presence (solid symbols) or absence (open symbols) of 100 nM AP20187. Mice that were given transplants of cultured cells received all the progeny generated in cultures initiated with 5 × 10 5 transduced cells at day 0. Each line depicts results from a single mouse. One mouse (6281) that was given transplants of cells cultured for 5 days in AP20187 was killed at day 174 after transplantation and 5 × 10 6 marrow cells from this mouse were transplanted into 2 lethally irradiated secondary recipients (symbols × and +). E indicates erythroid; P, platelets, G, granulocytes; M, monocytes; B, B cells; and T, T cells. (C; inserts) LAM-PCR using <t>Rsa</t> I confirms common provirus insertion patterns in the granulocytes (G), monocytes (M), B cells (B), and T cells (T) of mouse 6281. MW indicates DNA ladder. Similar results were obtained using 2 other restriction enzymes, <t>Tsp509</t> I and Hae III. Sequencing of LAM-PCR products from mouse 6281 (panel D) revealed provirus insertion sites at the indicated positions in chromosomes 4 and 16, and their presence in the indicated lineages was confirmed by PCR using using an LTR primer, a host genomic primer, and 38 cycles of amplification. A second independent experiment performed in serum-free conditions showed a similar trend at 64 days after transplantation.
    Rsa I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1005 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rsa i/product/New England Biolabs
    Average 99 stars, based on 1005 article reviews
    Price from $9.99 to $1999.99
    rsa i - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher rsa i restriction endonuclease
    <t>PCR-RFLP</t> (polymerase chain reaction-restriction fragment length polymorphism) <t>Rsa</t> I restriction patterns of amplified fliC genes of E. coli strains tested—example of results for 26 isolates. The numbers indicate the numbers of particular isolates. M: 100 bp DNA ladder.
    Rsa I Restriction Endonuclease, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rsa i restriction endonuclease/product/Thermo Fisher
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    rsa i restriction endonuclease - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    92
    Promega rsa i
    Representative diversity revealed by M46 PCR-RFLP in M. catarrhalis . (A) Amplimer B; (B) amplimer C. Samples were treated with Hae <t>III</t> (lanes 1 to 7) or with <t>Rsa</t> I (lanes 8 to 14) or are undigested amplimers (lanes 15 to 21). Each lane contained 10 μl of PCR product. Lanes 1, 8, and 15, sample ATCC 25238; lanes 2, 9, and 16, sample TN16a; lanes 3, 10, and 17, sample BL2; lanes 4, 11, and 18, sample NY1a; lanes 5, 12, and 19, sample TN17a; lanes 6, 13, and 20, sample TN7a; lanes 7, 14, and 21, sample TN13a. Lanes M are 100-to-1,500-bp ladders, with double-bright bands at 600 bp and additional fragments of 2,000 bp.
    Rsa I, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 569 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rsa i/product/Promega
    Average 92 stars, based on 569 article reviews
    Price from $9.99 to $1999.99
    rsa i - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    93
    Thermo Fisher rsa i
    A PCR-RFLP assay of the ITS2 region applied to pearls from Pinctada margaritifera , P. maxima and P. radiata . (A) PCR products of 575 bp ( P. margaritifera ), 571 bp ( P. maxima ) and 590–91 bp ( P. radiata ) obtained with ITS2 universal primers (5.8S-F and 28S-R) and (B) RFLP patterns of ITS2 amplicons (from A) obtained after digestion with <t>Rsa</t> I. MW: molecular weight size marker, 100-bp DNA ladder; lanes 1–3: P. maxima (PMX) pearls; lane 4: P. margaritifera (PMR) pearl; lanes 5–10: P. radiata (PR) pearls; lanes 11–16: P. margaritifera pearls; lane 17: PCR negative control; lanes 18 and 19: P. radiata and P. margaritifera positive controls. Note: The P. maxima positive control is shown in Figure 4 .
    Rsa I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 482 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rsa i/product/Thermo Fisher
    Average 93 stars, based on 482 article reviews
    Price from $9.99 to $1999.99
    rsa i - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    94
    Millipore rsa
    In vivo biodistribution of <t>ABD-fused</t> (Z HER2:342 ) 2 in a preclinical rat model. A , ELISA measurements showing pH-dependent binding of srFcRn to <t>RSA</t> in complex with ABD-(Z HER2:342 ) 2 . The numbers given represent the mean of triplicates. The blood, skin, and muscle biodistribution of 111 In-labeled-RSA ( B ) and 177 Lu-labeled-ABD-(Z HER2:342 ) 2 ( C ) in Sprague-Dawley rats (three rats/group and time point). Organ uptake is expressed as percent of injected radioactivity/g (% IA/g ), and error bars and corrected for interstitial volume as described under “Experimental Procedures.”
    Rsa, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 197 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rsa/product/Millipore
    Average 94 stars, based on 197 article reviews
    Price from $9.99 to $1999.99
    rsa - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    92
    Boehringer Mannheim rsa i
    In vivo biodistribution of <t>ABD-fused</t> (Z HER2:342 ) 2 in a preclinical rat model. A , ELISA measurements showing pH-dependent binding of srFcRn to <t>RSA</t> in complex with ABD-(Z HER2:342 ) 2 . The numbers given represent the mean of triplicates. The blood, skin, and muscle biodistribution of 111 In-labeled-RSA ( B ) and 177 Lu-labeled-ABD-(Z HER2:342 ) 2 ( C ) in Sprague-Dawley rats (three rats/group and time point). Organ uptake is expressed as percent of injected radioactivity/g (% IA/g ), and error bars and corrected for interstitial volume as described under “Experimental Procedures.”
    Rsa I, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rsa i/product/Boehringer Mannheim
    Average 92 stars, based on 53 article reviews
    Price from $9.99 to $1999.99
    rsa i - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    88
    Thermo Fisher restriction enzyme rsa i
    In vivo biodistribution of <t>ABD-fused</t> (Z HER2:342 ) 2 in a preclinical rat model. A , ELISA measurements showing pH-dependent binding of srFcRn to <t>RSA</t> in complex with ABD-(Z HER2:342 ) 2 . The numbers given represent the mean of triplicates. The blood, skin, and muscle biodistribution of 111 In-labeled-RSA ( B ) and 177 Lu-labeled-ABD-(Z HER2:342 ) 2 ( C ) in Sprague-Dawley rats (three rats/group and time point). Organ uptake is expressed as percent of injected radioactivity/g (% IA/g ), and error bars and corrected for interstitial volume as described under “Experimental Procedures.”
    Restriction Enzyme Rsa I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzyme rsa i/product/Thermo Fisher
    Average 88 stars, based on 85 article reviews
    Price from $9.99 to $1999.99
    restriction enzyme rsa i - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    88
    Promega rsa i restriction enzyme
    In vivo biodistribution of <t>ABD-fused</t> (Z HER2:342 ) 2 in a preclinical rat model. A , ELISA measurements showing pH-dependent binding of srFcRn to <t>RSA</t> in complex with ABD-(Z HER2:342 ) 2 . The numbers given represent the mean of triplicates. The blood, skin, and muscle biodistribution of 111 In-labeled-RSA ( B ) and 177 Lu-labeled-ABD-(Z HER2:342 ) 2 ( C ) in Sprague-Dawley rats (three rats/group and time point). Organ uptake is expressed as percent of injected radioactivity/g (% IA/g ), and error bars and corrected for interstitial volume as described under “Experimental Procedures.”
    Rsa I Restriction Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rsa i restriction enzyme/product/Promega
    Average 88 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    rsa i restriction enzyme - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    86
    Promega rsa i restriction endonucleases
    In vivo biodistribution of <t>ABD-fused</t> (Z HER2:342 ) 2 in a preclinical rat model. A , ELISA measurements showing pH-dependent binding of srFcRn to <t>RSA</t> in complex with ABD-(Z HER2:342 ) 2 . The numbers given represent the mean of triplicates. The blood, skin, and muscle biodistribution of 111 In-labeled-RSA ( B ) and 177 Lu-labeled-ABD-(Z HER2:342 ) 2 ( C ) in Sprague-Dawley rats (three rats/group and time point). Organ uptake is expressed as percent of injected radioactivity/g (% IA/g ), and error bars and corrected for interstitial volume as described under “Experimental Procedures.”
    Rsa I Restriction Endonucleases, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rsa i restriction endonucleases/product/Promega
    Average 86 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    rsa i restriction endonucleases - by Bioz Stars, 2020-09
    86/100 stars
      Buy from Supplier

    88
    Thermo Fisher rsa i restriction enzymes
    HRGβ2 regulates telomere length in human breast cancer cells A. , B. <t>DNA</t> from MCF-7 cells engineered to stably overexpress full-length human HRGβ 2 cDNA (MCF-7/T clones) and equivalent empty-vector (MCF-7/V control cells), and DNA from HRGβ 2 -overexpressing MDA-MB-231 and Hs578T cells and equivalent antisense AS-HRGβ 2 cells was isolated from collected cell pellets with inclusion of an RNase-A digestion step. Genomic DNA was digested with Hin fI/ Rsa I, and genomic blotting for telomeric fragments was carried out according to standard protocols (see “Materials and Methods” section for details). In-gel hybridization was performed using an end-labeled (TTAGGG) 3 probe following in situ denaturation of the DNA. Telomere gels were exposed to a Phosphorimager screen for 24 h and signals were quantified using ImageQuant software. Red bars indicate the median size of telomere length. The numbers on the left show the positions of a DNA-sizing ladder (in kb).
    Rsa I Restriction Enzymes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rsa i restriction enzymes/product/Thermo Fisher
    Average 88 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    rsa i restriction enzymes - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    86
    Thermo Fisher rsa i enzyme
    HRGβ2 regulates telomere length in human breast cancer cells A. , B. <t>DNA</t> from MCF-7 cells engineered to stably overexpress full-length human HRGβ 2 cDNA (MCF-7/T clones) and equivalent empty-vector (MCF-7/V control cells), and DNA from HRGβ 2 -overexpressing MDA-MB-231 and Hs578T cells and equivalent antisense AS-HRGβ 2 cells was isolated from collected cell pellets with inclusion of an RNase-A digestion step. Genomic DNA was digested with Hin fI/ Rsa I, and genomic blotting for telomeric fragments was carried out according to standard protocols (see “Materials and Methods” section for details). In-gel hybridization was performed using an end-labeled (TTAGGG) 3 probe following in situ denaturation of the DNA. Telomere gels were exposed to a Phosphorimager screen for 24 h and signals were quantified using ImageQuant software. Red bars indicate the median size of telomere length. The numbers on the left show the positions of a DNA-sizing ladder (in kb).
    Rsa I Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rsa i enzyme/product/Thermo Fisher
    Average 86 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    rsa i enzyme - by Bioz Stars, 2020-09
    86/100 stars
      Buy from Supplier

    99
    GE Healthcare rsa i
    Neighbour-joining similarity tree for 16S rRNA gene sequences of most mycobacterial species. Legend: N. asteroides ATCC 49872 (Genbank Z82229) was used as the outgroup. Table 1 lists the GenBank accession numbers of the sequences used to construct this tree. ARDRA patterns for Hha I, <t>Mbo</t> I, <t>Rsa</t> I and Bst UI are listed after the species name. a: GenBank AF028712. Erroneously listed in GenBank as M. peregrinum (see also legend of Table 1 ). b: M. gastri clusters below 100% with M. kansasii, although it is generally agreed that the 16S rRNA gene sequences for M. kansasii and M. gastri are identical. This can be explained by the fact that the only available GenBank M. gastri sequence (X52919) contained several ambiguities. c. M. lentiflavum, initially not included in the manuscript is not presented in this tree. It clusters close to the branch including M. heidelbergense, M. simiae, M. triplex and M. genavense.
    Rsa I, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rsa i/product/GE Healthcare
    Average 99 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    rsa i - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    86
    Promega enzyme rsa i
    Neighbour-joining similarity tree for 16S rRNA gene sequences of most mycobacterial species. Legend: N. asteroides ATCC 49872 (Genbank Z82229) was used as the outgroup. Table 1 lists the GenBank accession numbers of the sequences used to construct this tree. ARDRA patterns for Hha I, <t>Mbo</t> I, <t>Rsa</t> I and Bst UI are listed after the species name. a: GenBank AF028712. Erroneously listed in GenBank as M. peregrinum (see also legend of Table 1 ). b: M. gastri clusters below 100% with M. kansasii, although it is generally agreed that the 16S rRNA gene sequences for M. kansasii and M. gastri are identical. This can be explained by the fact that the only available GenBank M. gastri sequence (X52919) contained several ambiguities. c. M. lentiflavum, initially not included in the manuscript is not presented in this tree. It clusters close to the branch including M. heidelbergense, M. simiae, M. triplex and M. genavense.
    Enzyme Rsa I, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzyme rsa i/product/Promega
    Average 86 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    enzyme rsa i - by Bioz Stars, 2020-09
    86/100 stars
      Buy from Supplier

    88
    Promega rsa i restriction enzymes
    Neighbour-joining similarity tree for 16S rRNA gene sequences of most mycobacterial species. Legend: N. asteroides ATCC 49872 (Genbank Z82229) was used as the outgroup. Table 1 lists the GenBank accession numbers of the sequences used to construct this tree. ARDRA patterns for Hha I, <t>Mbo</t> I, <t>Rsa</t> I and Bst UI are listed after the species name. a: GenBank AF028712. Erroneously listed in GenBank as M. peregrinum (see also legend of Table 1 ). b: M. gastri clusters below 100% with M. kansasii, although it is generally agreed that the 16S rRNA gene sequences for M. kansasii and M. gastri are identical. This can be explained by the fact that the only available GenBank M. gastri sequence (X52919) contained several ambiguities. c. M. lentiflavum, initially not included in the manuscript is not presented in this tree. It clusters close to the branch including M. heidelbergense, M. simiae, M. triplex and M. genavense.
    Rsa I Restriction Enzymes, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rsa i restriction enzymes/product/Promega
    Average 88 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    rsa i restriction enzymes - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    92
    Toyobo rsa i
    Amplified products of the 16S rDNA of various Alex-CFPB (A) and digestion of the PCR products with <t>Rsa</t> I (B), Bst UI (C), <t>Mbo</t> I (D), and Hha I (E). Lane M, 100-bp ladder markers; lane 1, CFPB-A9; lane 2, CFPB-B1; lane 3, CFPB-C1; lane 4, CFPB-D1; lane 5, CFPB-E1; lane 6, CFPB-F1; lane 7, CFPB-G1; lane 8, CFPB-H1; lane 9, CFPB-I1; lane 10, CFPB-J1; lane 11, CFPB-K1; lane 12, CFPB-L1; lane 13, CFPB-M1; lane 14, CFPB-N1.
    Rsa I, supplied by Toyobo, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rsa i/product/Toyobo
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    rsa i - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    F36VFGFR1 supports the survival of long-term repopulating HSCs during growth factor deprivation. Marrow cells (5 × 10 5 ) transduced with the F36VFGFR1 vector were transplanted into lethally irradiated recipients either immediately following transduction (A) or after 1 (B) or 5 days (C) of culture in the presence (solid symbols) or absence (open symbols) of 100 nM AP20187. Mice that were given transplants of cultured cells received all the progeny generated in cultures initiated with 5 × 10 5 transduced cells at day 0. Each line depicts results from a single mouse. One mouse (6281) that was given transplants of cells cultured for 5 days in AP20187 was killed at day 174 after transplantation and 5 × 10 6 marrow cells from this mouse were transplanted into 2 lethally irradiated secondary recipients (symbols × and +). E indicates erythroid; P, platelets, G, granulocytes; M, monocytes; B, B cells; and T, T cells. (C; inserts) LAM-PCR using Rsa I confirms common provirus insertion patterns in the granulocytes (G), monocytes (M), B cells (B), and T cells (T) of mouse 6281. MW indicates DNA ladder. Similar results were obtained using 2 other restriction enzymes, Tsp509 I and Hae III. Sequencing of LAM-PCR products from mouse 6281 (panel D) revealed provirus insertion sites at the indicated positions in chromosomes 4 and 16, and their presence in the indicated lineages was confirmed by PCR using using an LTR primer, a host genomic primer, and 38 cycles of amplification. A second independent experiment performed in serum-free conditions showed a similar trend at 64 days after transplantation.

    Journal: Blood

    Article Title: Growth factor receptors as regulators of hematopoiesis

    doi: 10.1182/blood-2006-01-012278

    Figure Lengend Snippet: F36VFGFR1 supports the survival of long-term repopulating HSCs during growth factor deprivation. Marrow cells (5 × 10 5 ) transduced with the F36VFGFR1 vector were transplanted into lethally irradiated recipients either immediately following transduction (A) or after 1 (B) or 5 days (C) of culture in the presence (solid symbols) or absence (open symbols) of 100 nM AP20187. Mice that were given transplants of cultured cells received all the progeny generated in cultures initiated with 5 × 10 5 transduced cells at day 0. Each line depicts results from a single mouse. One mouse (6281) that was given transplants of cells cultured for 5 days in AP20187 was killed at day 174 after transplantation and 5 × 10 6 marrow cells from this mouse were transplanted into 2 lethally irradiated secondary recipients (symbols × and +). E indicates erythroid; P, platelets, G, granulocytes; M, monocytes; B, B cells; and T, T cells. (C; inserts) LAM-PCR using Rsa I confirms common provirus insertion patterns in the granulocytes (G), monocytes (M), B cells (B), and T cells (T) of mouse 6281. MW indicates DNA ladder. Similar results were obtained using 2 other restriction enzymes, Tsp509 I and Hae III. Sequencing of LAM-PCR products from mouse 6281 (panel D) revealed provirus insertion sites at the indicated positions in chromosomes 4 and 16, and their presence in the indicated lineages was confirmed by PCR using using an LTR primer, a host genomic primer, and 38 cycles of amplification. A second independent experiment performed in serum-free conditions showed a similar trend at 64 days after transplantation.

    Article Snippet: Products were divided into 3 equal arms and digested with Tsp509 I, Hae III, or Rsa I (New England BioLabs).

    Techniques: Transduction, Plasmid Preparation, Irradiation, Mouse Assay, Cell Culture, Generated, Transplantation Assay, Laser Capture Microdissection, Polymerase Chain Reaction, Sequencing, Amplification

    PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) Rsa I restriction patterns of amplified fliC genes of E. coli strains tested—example of results for 26 isolates. The numbers indicate the numbers of particular isolates. M: 100 bp DNA ladder.

    Journal: Pathogens

    Article Title: New Approaches for Escherichia coli Genotyping

    doi: 10.3390/pathogens9020073

    Figure Lengend Snippet: PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) Rsa I restriction patterns of amplified fliC genes of E. coli strains tested—example of results for 26 isolates. The numbers indicate the numbers of particular isolates. M: 100 bp DNA ladder.

    Article Snippet: Final elongation was performed at 72 °C for 5 min. PCR products were digested with Rsa I restriction endonuclease (Thermo Fischer Scientific, Waltham, MA, USA) according to the manufacturer’s instruction.

    Techniques: Polymerase Chain Reaction, Amplification

    Representative diversity revealed by M46 PCR-RFLP in M. catarrhalis . (A) Amplimer B; (B) amplimer C. Samples were treated with Hae III (lanes 1 to 7) or with Rsa I (lanes 8 to 14) or are undigested amplimers (lanes 15 to 21). Each lane contained 10 μl of PCR product. Lanes 1, 8, and 15, sample ATCC 25238; lanes 2, 9, and 16, sample TN16a; lanes 3, 10, and 17, sample BL2; lanes 4, 11, and 18, sample NY1a; lanes 5, 12, and 19, sample TN17a; lanes 6, 13, and 20, sample TN7a; lanes 7, 14, and 21, sample TN13a. Lanes M are 100-to-1,500-bp ladders, with double-bright bands at 600 bp and additional fragments of 2,000 bp.

    Journal: Journal of Clinical Microbiology

    Article Title: Genetic Diversity among Strains of Moraxella catarrhalis: Analysis Using Multiple DNA Probes and a Single-Locus PCR-Restriction Fragment Length Polymorphism Method

    doi:

    Figure Lengend Snippet: Representative diversity revealed by M46 PCR-RFLP in M. catarrhalis . (A) Amplimer B; (B) amplimer C. Samples were treated with Hae III (lanes 1 to 7) or with Rsa I (lanes 8 to 14) or are undigested amplimers (lanes 15 to 21). Each lane contained 10 μl of PCR product. Lanes 1, 8, and 15, sample ATCC 25238; lanes 2, 9, and 16, sample TN16a; lanes 3, 10, and 17, sample BL2; lanes 4, 11, and 18, sample NY1a; lanes 5, 12, and 19, sample TN17a; lanes 6, 13, and 20, sample TN7a; lanes 7, 14, and 21, sample TN13a. Lanes M are 100-to-1,500-bp ladders, with double-bright bands at 600 bp and additional fragments of 2,000 bp.

    Article Snippet: To detect RFLP variation within PCR-amplified regions encompassed by M46 DNA, 10-μl aliquots of PCR products were subjected to separate restriction enzyme digestion with Hae III and Rsa I (Promega Corp.) according to the manufacturer’s instructions.

    Techniques: Polymerase Chain Reaction

    A PCR-RFLP assay of the ITS2 region applied to pearls from Pinctada margaritifera , P. maxima and P. radiata . (A) PCR products of 575 bp ( P. margaritifera ), 571 bp ( P. maxima ) and 590–91 bp ( P. radiata ) obtained with ITS2 universal primers (5.8S-F and 28S-R) and (B) RFLP patterns of ITS2 amplicons (from A) obtained after digestion with Rsa I. MW: molecular weight size marker, 100-bp DNA ladder; lanes 1–3: P. maxima (PMX) pearls; lane 4: P. margaritifera (PMR) pearl; lanes 5–10: P. radiata (PR) pearls; lanes 11–16: P. margaritifera pearls; lane 17: PCR negative control; lanes 18 and 19: P. radiata and P. margaritifera positive controls. Note: The P. maxima positive control is shown in Figure 4 .

    Journal: PLoS ONE

    Article Title: DNA Fingerprinting of Pearls to Determine Their Origins

    doi: 10.1371/journal.pone.0075606

    Figure Lengend Snippet: A PCR-RFLP assay of the ITS2 region applied to pearls from Pinctada margaritifera , P. maxima and P. radiata . (A) PCR products of 575 bp ( P. margaritifera ), 571 bp ( P. maxima ) and 590–91 bp ( P. radiata ) obtained with ITS2 universal primers (5.8S-F and 28S-R) and (B) RFLP patterns of ITS2 amplicons (from A) obtained after digestion with Rsa I. MW: molecular weight size marker, 100-bp DNA ladder; lanes 1–3: P. maxima (PMX) pearls; lane 4: P. margaritifera (PMR) pearl; lanes 5–10: P. radiata (PR) pearls; lanes 11–16: P. margaritifera pearls; lane 17: PCR negative control; lanes 18 and 19: P. radiata and P. margaritifera positive controls. Note: The P. maxima positive control is shown in Figure 4 .

    Article Snippet: Restriction analysis was done in 12 µl reaction mixtures with 5 µl of amplified product, 100 µg/ml bovine serum albumin (New England Biolabs, Inc.), 1.2 µl enzyme buffer (New England Biolabs, Inc.) and 0.5 units of Rsa I (Fermentas GmbH).

    Techniques: Polymerase Chain Reaction, RFLP Assay, Molecular Weight, Marker, Negative Control, Positive Control

    Blind PCR-RFLP assay with eighteen pearls of unknown identity. (A) PCR products of 575 bp ( Pinctada margaritifera ), 571 bp ( P. maxima ) and 590–91 bp ( P. radiata ) obtained with ITS2 universal primers (5.8S-F and 28S-R) and (B) of 335 bp obtained with 28S-R and the P. margaritifera specific primer ITS2-Marg-F. (C) RFLP patterns of ITS2 gene fragments (from A) obtained after digestion with Rsa I. MW: molecular weight size marker, 100 bp DNA ladder; lanes 1–18: pearl isolates; lanes 19–20: DNA extraction negative controls; lane 21: PCR negative control; lanes 22–23: P. radiata and P. margaritifera positive controls; lanes 24–26: P. radiata , P. margaritifera and P. maxima positive controls showing ITS2 PCR products (upper gel) and ITS2-RFLP patterns (lower gel).

    Journal: PLoS ONE

    Article Title: DNA Fingerprinting of Pearls to Determine Their Origins

    doi: 10.1371/journal.pone.0075606

    Figure Lengend Snippet: Blind PCR-RFLP assay with eighteen pearls of unknown identity. (A) PCR products of 575 bp ( Pinctada margaritifera ), 571 bp ( P. maxima ) and 590–91 bp ( P. radiata ) obtained with ITS2 universal primers (5.8S-F and 28S-R) and (B) of 335 bp obtained with 28S-R and the P. margaritifera specific primer ITS2-Marg-F. (C) RFLP patterns of ITS2 gene fragments (from A) obtained after digestion with Rsa I. MW: molecular weight size marker, 100 bp DNA ladder; lanes 1–18: pearl isolates; lanes 19–20: DNA extraction negative controls; lane 21: PCR negative control; lanes 22–23: P. radiata and P. margaritifera positive controls; lanes 24–26: P. radiata , P. margaritifera and P. maxima positive controls showing ITS2 PCR products (upper gel) and ITS2-RFLP patterns (lower gel).

    Article Snippet: Restriction analysis was done in 12 µl reaction mixtures with 5 µl of amplified product, 100 µg/ml bovine serum albumin (New England Biolabs, Inc.), 1.2 µl enzyme buffer (New England Biolabs, Inc.) and 0.5 units of Rsa I (Fermentas GmbH).

    Techniques: Polymerase Chain Reaction, RFLP Assay, Molecular Weight, Marker, DNA Extraction, Negative Control

    In vivo biodistribution of ABD-fused (Z HER2:342 ) 2 in a preclinical rat model. A , ELISA measurements showing pH-dependent binding of srFcRn to RSA in complex with ABD-(Z HER2:342 ) 2 . The numbers given represent the mean of triplicates. The blood, skin, and muscle biodistribution of 111 In-labeled-RSA ( B ) and 177 Lu-labeled-ABD-(Z HER2:342 ) 2 ( C ) in Sprague-Dawley rats (three rats/group and time point). Organ uptake is expressed as percent of injected radioactivity/g (% IA/g ), and error bars and corrected for interstitial volume as described under “Experimental Procedures.”

    Journal: The Journal of Biological Chemistry

    Article Title: Extending Half-life by Indirect Targeting of the Neonatal Fc Receptor (FcRn) Using a Minimal Albumin Binding Domain *

    doi: 10.1074/jbc.M110.164848

    Figure Lengend Snippet: In vivo biodistribution of ABD-fused (Z HER2:342 ) 2 in a preclinical rat model. A , ELISA measurements showing pH-dependent binding of srFcRn to RSA in complex with ABD-(Z HER2:342 ) 2 . The numbers given represent the mean of triplicates. The blood, skin, and muscle biodistribution of 111 In-labeled-RSA ( B ) and 177 Lu-labeled-ABD-(Z HER2:342 ) 2 ( C ) in Sprague-Dawley rats (three rats/group and time point). Organ uptake is expressed as percent of injected radioactivity/g (% IA/g ), and error bars and corrected for interstitial volume as described under “Experimental Procedures.”

    Article Snippet: The chelator CHX-A″/DTPA (Macrocyclics, Dallas, TX) was coupled to RSA (Sigma) or ABD-(ZHER2:342 )2 at a 1:1 molar ratio to avoid overmodification; 200 μl of RSA (5 mg/ml in 0.07 m borate buffer, pH 9.2) was mixed with 10 μl of a freshly prepared solution of CHX-A″/DTPA (1 mg/ml in 0.07 m borate buffer, pH 9.2), and 290 μl of 0.07 m borate buffer, pH 9.2, was added to the mixture.

    Techniques: In Vivo, Enzyme-linked Immunosorbent Assay, Binding Assay, Labeling, Injection, Radioactivity, IA

    ( A ) Image of 96 Rsa I-digested HFE amplicons analyzed in two sequential 48-sample separations. ( B and C ) Expanded views of the separation image from the first and second injection, respectively. The total time for the two separations was less than 7.6 min. Samples were separated on 0.75% (wt/vol) hydroxyethylcellulose in 1× TBE buffer with 1 μM ethidium bromide in the running buffer. The channels were 10 cm in length from the injection to the detection region. The injection was performed as described in the text and the applied fields for injection and separation were 300 V/cm.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: High-throughput genetic analysis using microfabricated 96-sample capillary array electrophoresis microplates

    doi:

    Figure Lengend Snippet: ( A ) Image of 96 Rsa I-digested HFE amplicons analyzed in two sequential 48-sample separations. ( B and C ) Expanded views of the separation image from the first and second injection, respectively. The total time for the two separations was less than 7.6 min. Samples were separated on 0.75% (wt/vol) hydroxyethylcellulose in 1× TBE buffer with 1 μM ethidium bromide in the running buffer. The channels were 10 cm in length from the injection to the detection region. The injection was performed as described in the text and the applied fields for injection and separation were 300 V/cm.

    Article Snippet: The restriction digestion of amplified product was carried out by adding 4 μl of each amplified sample to 6 μl of buffer containing 2 units of Rsa I (Sigma) and digesting for 90 min at 37°C.

    Techniques: Injection

    Spatial evolution of phylotype richness ( S ) and Shannon-Weiner indexes ( H ) for eubacterial ( S eub , H eub ; MspI digestion) and archaeal ( S arch , H arch ; RsaI digestion) communities.

    Journal: Applied and Environmental Microbiology

    Article Title: Anaerobic Microbial Communities in Lake Pavin, a Unique Meromictic Lake in France †

    doi: 10.1128/AEM.71.11.7389-7400.2005

    Figure Lengend Snippet: Spatial evolution of phylotype richness ( S ) and Shannon-Weiner indexes ( H ) for eubacterial ( S eub , H eub ; MspI digestion) and archaeal ( S arch , H arch ; RsaI digestion) communities.

    Article Snippet: The 25-μl reaction mixtures contained 20 U of RsaI, MspI, and HhaI (Sigma) in the manufacturer's recommended reaction buffer.

    Techniques:

    Relative abundances of TRFs from bacterial (A) and archaeal (B) 16S rRNA genes. Diagrams show the results after cleavage with MspI (bacteria) and RsaI (archaea). Numbers in the keys indicate the lengths of the TRFs, in base pairs, for fragments with a relative abundance of > 1%.

    Journal: Applied and Environmental Microbiology

    Article Title: Anaerobic Microbial Communities in Lake Pavin, a Unique Meromictic Lake in France †

    doi: 10.1128/AEM.71.11.7389-7400.2005

    Figure Lengend Snippet: Relative abundances of TRFs from bacterial (A) and archaeal (B) 16S rRNA genes. Diagrams show the results after cleavage with MspI (bacteria) and RsaI (archaea). Numbers in the keys indicate the lengths of the TRFs, in base pairs, for fragments with a relative abundance of > 1%.

    Article Snippet: The 25-μl reaction mixtures contained 20 U of RsaI, MspI, and HhaI (Sigma) in the manufacturer's recommended reaction buffer.

    Techniques:

    COA analyses of TRF relationships between sample locations. (a) Eubacterial TRFs from MspI cleavages. (b) Archaeal TRFs from RsaI cleavages.

    Journal: Applied and Environmental Microbiology

    Article Title: Anaerobic Microbial Communities in Lake Pavin, a Unique Meromictic Lake in France †

    doi: 10.1128/AEM.71.11.7389-7400.2005

    Figure Lengend Snippet: COA analyses of TRF relationships between sample locations. (a) Eubacterial TRFs from MspI cleavages. (b) Archaeal TRFs from RsaI cleavages.

    Article Snippet: The 25-μl reaction mixtures contained 20 U of RsaI, MspI, and HhaI (Sigma) in the manufacturer's recommended reaction buffer.

    Techniques:

    MMP-2 activity, AGE accumulation and RAGE expression in the renal tubules of diabetic or AGE-RSA-infused rats. (A) MMP-2 activity in 32-week Ctrl or diabetic renal tubules with or without ramipril treatment was determined by gelatin zymography ( n = 4). (B) AGE protein levels in 32-week Ctrl or diabetic renal tubules with or without ramipril treatment were evaluated by enzyme-linked immunosorbent assay for carboxymethyllisine ( n = 7). (C) RAGE protein expression in 32-week Ctrl or diabetic renal tubules with or without ramipril treatment was determined by western blot. Data were normalized by the intensity of α-tubulin bands. ( n = 4). (D) RAGE gene expression in total kidney cortex of AGE-RSA- or RSA-infused rats was evaluated by quantitative real-time RT-PCR (n = 7). (E) MMP-2 activity in the tubules of AGE-RSA- or RSA-infused rats was determined by gelatin zymography (n = 4). Data are shown as mean ± SEM.

    Journal: Diabetology & Metabolic Syndrome

    Article Title: Ramipril inhibits AGE-RAGE-induced matrix metalloproteinase-2 activation in experimental diabetic nephropathy

    doi: 10.1186/1758-5996-6-86

    Figure Lengend Snippet: MMP-2 activity, AGE accumulation and RAGE expression in the renal tubules of diabetic or AGE-RSA-infused rats. (A) MMP-2 activity in 32-week Ctrl or diabetic renal tubules with or without ramipril treatment was determined by gelatin zymography ( n = 4). (B) AGE protein levels in 32-week Ctrl or diabetic renal tubules with or without ramipril treatment were evaluated by enzyme-linked immunosorbent assay for carboxymethyllisine ( n = 7). (C) RAGE protein expression in 32-week Ctrl or diabetic renal tubules with or without ramipril treatment was determined by western blot. Data were normalized by the intensity of α-tubulin bands. ( n = 4). (D) RAGE gene expression in total kidney cortex of AGE-RSA- or RSA-infused rats was evaluated by quantitative real-time RT-PCR (n = 7). (E) MMP-2 activity in the tubules of AGE-RSA- or RSA-infused rats was determined by gelatin zymography (n = 4). Data are shown as mean ± SEM.

    Article Snippet: Preparation of AGE-RSA and AGE-modified bovine serum albumin (AGE-BSA) AGE-RSA and AGE-BSA were prepared by incubating RSA or BSA (Fraction V, Sigma Chemical Co, St. Louis, MO, USA) with 0.5 M D-glucose in PBS at 37°C for 3 months as previously described [ ].

    Techniques: Activity Assay, Expressing, Zymography, Enzyme-linked Immunosorbent Assay, Western Blot, Quantitative RT-PCR

    MMP-2 activity and gene expression, and intracellular ROS generation in RPTCs. (A, B) RPTCs were stimulated with 100 μg/ml AGE-BSA or non-glycated BSA with or without 10 -7 M ramiprilat or 100 nM BAY11-7082 for 48 hr, and MMP-2 activity in the supernatant was determined by gelatin zymography (n = 4). (C) Intracellular ROS generation in RPTCs. Cells were stimulated with 100 μg/ml AGE-BSA or BSA with or without 10 -7 M ramiprilat for 24 hr, then the intracellular ROS generation in RPTCs was evaluated using fluorescent probe 10 μM CM-H 2 DCFDA. (D) Effects of Ang II on MMP-2 gene expression in RPTCs. The cells were stimulated with 10 -6 M Ang II with or without 100 nM BAY11-7082 for 24 hr and MMP-2 gene expression was evaluated by quantitative real-time RT-PCR (n = 6). Data are shown as mean ± SEM.

    Journal: Diabetology & Metabolic Syndrome

    Article Title: Ramipril inhibits AGE-RAGE-induced matrix metalloproteinase-2 activation in experimental diabetic nephropathy

    doi: 10.1186/1758-5996-6-86

    Figure Lengend Snippet: MMP-2 activity and gene expression, and intracellular ROS generation in RPTCs. (A, B) RPTCs were stimulated with 100 μg/ml AGE-BSA or non-glycated BSA with or without 10 -7 M ramiprilat or 100 nM BAY11-7082 for 48 hr, and MMP-2 activity in the supernatant was determined by gelatin zymography (n = 4). (C) Intracellular ROS generation in RPTCs. Cells were stimulated with 100 μg/ml AGE-BSA or BSA with or without 10 -7 M ramiprilat for 24 hr, then the intracellular ROS generation in RPTCs was evaluated using fluorescent probe 10 μM CM-H 2 DCFDA. (D) Effects of Ang II on MMP-2 gene expression in RPTCs. The cells were stimulated with 10 -6 M Ang II with or without 100 nM BAY11-7082 for 24 hr and MMP-2 gene expression was evaluated by quantitative real-time RT-PCR (n = 6). Data are shown as mean ± SEM.

    Article Snippet: Preparation of AGE-RSA and AGE-modified bovine serum albumin (AGE-BSA) AGE-RSA and AGE-BSA were prepared by incubating RSA or BSA (Fraction V, Sigma Chemical Co, St. Louis, MO, USA) with 0.5 M D-glucose in PBS at 37°C for 3 months as previously described [ ].

    Techniques: Activity Assay, Expressing, Zymography, Quantitative RT-PCR

    HRGβ2 regulates telomere length in human breast cancer cells A. , B. DNA from MCF-7 cells engineered to stably overexpress full-length human HRGβ 2 cDNA (MCF-7/T clones) and equivalent empty-vector (MCF-7/V control cells), and DNA from HRGβ 2 -overexpressing MDA-MB-231 and Hs578T cells and equivalent antisense AS-HRGβ 2 cells was isolated from collected cell pellets with inclusion of an RNase-A digestion step. Genomic DNA was digested with Hin fI/ Rsa I, and genomic blotting for telomeric fragments was carried out according to standard protocols (see “Materials and Methods” section for details). In-gel hybridization was performed using an end-labeled (TTAGGG) 3 probe following in situ denaturation of the DNA. Telomere gels were exposed to a Phosphorimager screen for 24 h and signals were quantified using ImageQuant software. Red bars indicate the median size of telomere length. The numbers on the left show the positions of a DNA-sizing ladder (in kb).

    Journal: Oncotarget

    Article Title: Heregulin, a new regulator of telomere length in human cells

    doi:

    Figure Lengend Snippet: HRGβ2 regulates telomere length in human breast cancer cells A. , B. DNA from MCF-7 cells engineered to stably overexpress full-length human HRGβ 2 cDNA (MCF-7/T clones) and equivalent empty-vector (MCF-7/V control cells), and DNA from HRGβ 2 -overexpressing MDA-MB-231 and Hs578T cells and equivalent antisense AS-HRGβ 2 cells was isolated from collected cell pellets with inclusion of an RNase-A digestion step. Genomic DNA was digested with Hin fI/ Rsa I, and genomic blotting for telomeric fragments was carried out according to standard protocols (see “Materials and Methods” section for details). In-gel hybridization was performed using an end-labeled (TTAGGG) 3 probe following in situ denaturation of the DNA. Telomere gels were exposed to a Phosphorimager screen for 24 h and signals were quantified using ImageQuant software. Red bars indicate the median size of telomere length. The numbers on the left show the positions of a DNA-sizing ladder (in kb).

    Article Snippet: Protein-free DNA was cleaved with Hinf I and Rsa I restriction enzymes (Gibco/BRL), extracted once with phenol/chloroform/isoamyl alcohol, ethanol precipitated, and resuspended in Tris-EDTA to generate the TRF.

    Techniques: Stable Transfection, Plasmid Preparation, Multiple Displacement Amplification, Isolation, Hybridization, Labeling, In Situ, Software

    A. Retrovirally-induced HRGβ2 regulates TRF2 and RAP1 expression and telomere length in MCF-7 breast cancer cells. Left. Representative immunoblot ( n = 3 ) showing upregulation of TRF2 and RAP1 in MCF-7 cells retrovirally engineered to overexpress HRGβ 2 . PD 0 represents the first sub-passage after selection for retroviral infection. At the indicated PDs, cells were washed with cold PBS and solubilized in lysis buffer containing phosphatase and protease inhibitors. Fifty μg of protein per sample was resolved by SDS-PAGE and subjected to Western blotting for TRF2 and hRap1 as described above (see “Materials and Methods” for details). Middle panel. MCF-7/HRGβ 2 cells do not exhibit significant changes in telomerase activity as assessed by the TRAP assay. Three thousand cell equivalents were used for each reaction and a representative experiment is shown ( n = 3 ). Right panel. Telomere length changes in MCF-7 cells upon retrovirally-induced HRGβ 2 overexpression. The panel shows a representative genomic blotting analysis of telomeric restriction fragments in Hin fI/ Rsa I-digested genomic DNA from retrovirally-infected MCF-7 cells probed with a TTAGGG repeat fragment. B. HRG knockdown reduces the presence of TRF2 and RAP1 on telomeres and promotes telomere lengthening. Top left. Depletion of HRG with siRNA significantly affects TRF2 and RAP1 protein levels. A representative Western blot of MDA-MB-231 cell lysates 72 h after transfection of siRNA to HRG or siRNA control is shown ( n = 3 ). Top right. TRF length analysis in HRG knockdown MDA-MB-231 cells. The panel shows a representative genomic blotting analysis of telomeric restriction fragments in Hin fI/ Rsa I-digested genomic DNA from MDA-MB-231 cells transiently transfected with control siRNA or graded concentrations of HRG siRNA (PD 4) probed with a TTAGGG repeat fragment ( n = 3 ). Bottom. Reduced TRF2 and RAP1 telomeric signals after HRG siRNA treatment. Western blot analysis showing the significant depletion of HRGβ 2 protein with a specific siRNA. The panel shows representative immunofluorescence images of MDA-MB-231 cells 48 h after introduction of HRG siRNA: staining with anti-TRF2 ( red ) and anti-RAP1 ( green ) antibodies is shown ( n = 3 ). DAPI was used to visualize nuclear DNA ( blue ).

    Journal: Oncotarget

    Article Title: Heregulin, a new regulator of telomere length in human cells

    doi:

    Figure Lengend Snippet: A. Retrovirally-induced HRGβ2 regulates TRF2 and RAP1 expression and telomere length in MCF-7 breast cancer cells. Left. Representative immunoblot ( n = 3 ) showing upregulation of TRF2 and RAP1 in MCF-7 cells retrovirally engineered to overexpress HRGβ 2 . PD 0 represents the first sub-passage after selection for retroviral infection. At the indicated PDs, cells were washed with cold PBS and solubilized in lysis buffer containing phosphatase and protease inhibitors. Fifty μg of protein per sample was resolved by SDS-PAGE and subjected to Western blotting for TRF2 and hRap1 as described above (see “Materials and Methods” for details). Middle panel. MCF-7/HRGβ 2 cells do not exhibit significant changes in telomerase activity as assessed by the TRAP assay. Three thousand cell equivalents were used for each reaction and a representative experiment is shown ( n = 3 ). Right panel. Telomere length changes in MCF-7 cells upon retrovirally-induced HRGβ 2 overexpression. The panel shows a representative genomic blotting analysis of telomeric restriction fragments in Hin fI/ Rsa I-digested genomic DNA from retrovirally-infected MCF-7 cells probed with a TTAGGG repeat fragment. B. HRG knockdown reduces the presence of TRF2 and RAP1 on telomeres and promotes telomere lengthening. Top left. Depletion of HRG with siRNA significantly affects TRF2 and RAP1 protein levels. A representative Western blot of MDA-MB-231 cell lysates 72 h after transfection of siRNA to HRG or siRNA control is shown ( n = 3 ). Top right. TRF length analysis in HRG knockdown MDA-MB-231 cells. The panel shows a representative genomic blotting analysis of telomeric restriction fragments in Hin fI/ Rsa I-digested genomic DNA from MDA-MB-231 cells transiently transfected with control siRNA or graded concentrations of HRG siRNA (PD 4) probed with a TTAGGG repeat fragment ( n = 3 ). Bottom. Reduced TRF2 and RAP1 telomeric signals after HRG siRNA treatment. Western blot analysis showing the significant depletion of HRGβ 2 protein with a specific siRNA. The panel shows representative immunofluorescence images of MDA-MB-231 cells 48 h after introduction of HRG siRNA: staining with anti-TRF2 ( red ) and anti-RAP1 ( green ) antibodies is shown ( n = 3 ). DAPI was used to visualize nuclear DNA ( blue ).

    Article Snippet: Protein-free DNA was cleaved with Hinf I and Rsa I restriction enzymes (Gibco/BRL), extracted once with phenol/chloroform/isoamyl alcohol, ethanol precipitated, and resuspended in Tris-EDTA to generate the TRF.

    Techniques: Expressing, Selection, Infection, Lysis, SDS Page, Western Blot, Activity Assay, TRAP Assay, Over Expression, Multiple Displacement Amplification, Transfection, Immunofluorescence, Staining

    Neighbour-joining similarity tree for 16S rRNA gene sequences of most mycobacterial species. Legend: N. asteroides ATCC 49872 (Genbank Z82229) was used as the outgroup. Table 1 lists the GenBank accession numbers of the sequences used to construct this tree. ARDRA patterns for Hha I, Mbo I, Rsa I and Bst UI are listed after the species name. a: GenBank AF028712. Erroneously listed in GenBank as M. peregrinum (see also legend of Table 1 ). b: M. gastri clusters below 100% with M. kansasii, although it is generally agreed that the 16S rRNA gene sequences for M. kansasii and M. gastri are identical. This can be explained by the fact that the only available GenBank M. gastri sequence (X52919) contained several ambiguities. c. M. lentiflavum, initially not included in the manuscript is not presented in this tree. It clusters close to the branch including M. heidelbergense, M. simiae, M. triplex and M. genavense.

    Journal: BMC Microbiology

    Article Title: Evaluation of amplified rDNA restriction analysis (ARDRA) for the identification of cultured mycobacteria in a diagnostic laboratory

    doi: 10.1186/1471-2180-2-4

    Figure Lengend Snippet: Neighbour-joining similarity tree for 16S rRNA gene sequences of most mycobacterial species. Legend: N. asteroides ATCC 49872 (Genbank Z82229) was used as the outgroup. Table 1 lists the GenBank accession numbers of the sequences used to construct this tree. ARDRA patterns for Hha I, Mbo I, Rsa I and Bst UI are listed after the species name. a: GenBank AF028712. Erroneously listed in GenBank as M. peregrinum (see also legend of Table 1 ). b: M. gastri clusters below 100% with M. kansasii, although it is generally agreed that the 16S rRNA gene sequences for M. kansasii and M. gastri are identical. This can be explained by the fact that the only available GenBank M. gastri sequence (X52919) contained several ambiguities. c. M. lentiflavum, initially not included in the manuscript is not presented in this tree. It clusters close to the branch including M. heidelbergense, M. simiae, M. triplex and M. genavense.

    Article Snippet: Restriction digestion The restriction enzymes used were Hha I (isoschizomer of CfoI)(Amersham Pharmacia Biotech Benelux, Roosendaal, the Netherlands), Mbo I (Fermentas, Vilnius, Lithuania), Rsa I (Amersham Pharmacia).

    Techniques: Construct, Sequencing

    Rsa I restriction patterns of amplified mycobacterial 16S rRNA genes. Legend: M: marker (100 base pair ladder, Fermentas, Vilnius, Lithuania)

    Journal: BMC Microbiology

    Article Title: Evaluation of amplified rDNA restriction analysis (ARDRA) for the identification of cultured mycobacteria in a diagnostic laboratory

    doi: 10.1186/1471-2180-2-4

    Figure Lengend Snippet: Rsa I restriction patterns of amplified mycobacterial 16S rRNA genes. Legend: M: marker (100 base pair ladder, Fermentas, Vilnius, Lithuania)

    Article Snippet: Restriction digestion The restriction enzymes used were Hha I (isoschizomer of CfoI)(Amersham Pharmacia Biotech Benelux, Roosendaal, the Netherlands), Mbo I (Fermentas, Vilnius, Lithuania), Rsa I (Amersham Pharmacia).

    Techniques: Amplification, Marker

    Rsa I restriction patterns of mycobacterial 16S rRNA genes, theoretically calculated using RFLP (Applied Maths) and published GenBank sequences. Graphical representation and table of restriction fragment lengths for each of the possible patterns.

    Journal: BMC Microbiology

    Article Title: Evaluation of amplified rDNA restriction analysis (ARDRA) for the identification of cultured mycobacteria in a diagnostic laboratory

    doi: 10.1186/1471-2180-2-4

    Figure Lengend Snippet: Rsa I restriction patterns of mycobacterial 16S rRNA genes, theoretically calculated using RFLP (Applied Maths) and published GenBank sequences. Graphical representation and table of restriction fragment lengths for each of the possible patterns.

    Article Snippet: Restriction digestion The restriction enzymes used were Hha I (isoschizomer of CfoI)(Amersham Pharmacia Biotech Benelux, Roosendaal, the Netherlands), Mbo I (Fermentas, Vilnius, Lithuania), Rsa I (Amersham Pharmacia).

    Techniques:

    Amplified products of the 16S rDNA of various Alex-CFPB (A) and digestion of the PCR products with Rsa I (B), Bst UI (C), Mbo I (D), and Hha I (E). Lane M, 100-bp ladder markers; lane 1, CFPB-A9; lane 2, CFPB-B1; lane 3, CFPB-C1; lane 4, CFPB-D1; lane 5, CFPB-E1; lane 6, CFPB-F1; lane 7, CFPB-G1; lane 8, CFPB-H1; lane 9, CFPB-I1; lane 10, CFPB-J1; lane 11, CFPB-K1; lane 12, CFPB-L1; lane 13, CFPB-M1; lane 14, CFPB-N1.

    Journal: Applied and Environmental Microbiology

    Article Title: Population Structure of Alexandrium (Dinophyceae) Cyst Formation-Promoting Bacteria in Hiroshima Bay, Japan

    doi: 10.1128/AEM.69.11.6560-6568.2003

    Figure Lengend Snippet: Amplified products of the 16S rDNA of various Alex-CFPB (A) and digestion of the PCR products with Rsa I (B), Bst UI (C), Mbo I (D), and Hha I (E). Lane M, 100-bp ladder markers; lane 1, CFPB-A9; lane 2, CFPB-B1; lane 3, CFPB-C1; lane 4, CFPB-D1; lane 5, CFPB-E1; lane 6, CFPB-F1; lane 7, CFPB-G1; lane 8, CFPB-H1; lane 9, CFPB-I1; lane 10, CFPB-J1; lane 11, CFPB-K1; lane 12, CFPB-L1; lane 13, CFPB-M1; lane 14, CFPB-N1.

    Article Snippet: Two micrograms of the DNA was then digested with one of four restriction endonucleases, Mbo I, Hha I (TaKaRa Biomedicals, Osaka, Japan), Rsa I (Toyobo, Osaka, Japan), and Bst UI (New England Biolabs, Inc., Beverly, Mass.) according to the protocols of each manufacturer.

    Techniques: Amplification, Polymerase Chain Reaction