rr1 bacterial cells Search Results


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  • 96
    ATCC hybridoma cell line
    Hybridoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 426 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher competent cells
    Competent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1082 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    ATCC genome sequencing escherichia coli strain rr1
    Pedigree of <t>Escherichia</t> coli <t>RR1</t> and other related strains . Newly introduced mutations are denoted after a plus sign. Lineage from wild-type E. coli K-12 to W2961 was based on Bachmann (1972 , 1996 ). Lineage from W2961 to HB101 was based on Boyer and Roulland-Dussoix (1969) , and Rothen (1997) . Linage from wild-type K-12 to MG1655 was based on Bachmann (1996) and Hayashi et al. (2006) . Lineage from wild-type B to AC2516 was based on Boyer (1964) and Rothen (1997) . Conjugal transfer of genes is denoted by dashed arrows. AC2515 is a Salmonella typhosa strain carrying F- lac from E. coli ( Johnson et al., 1964 ). KLF4 is an F′ factor ( Low, 1968 ) that was derived from AB259, an Hfr (Hayes) strain. Note that rpoS (Am) and rph-1 mutations do not appear in Bachmann (1972 , 1996 ). AO and EMB indicate acridine orange and eosin methylene blue agar, respectively.
    Genome Sequencing Escherichia Coli Strain Rr1, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen maxi extraction kit
    Pedigree of <t>Escherichia</t> coli <t>RR1</t> and other related strains . Newly introduced mutations are denoted after a plus sign. Lineage from wild-type E. coli K-12 to W2961 was based on Bachmann (1972 , 1996 ). Lineage from W2961 to HB101 was based on Boyer and Roulland-Dussoix (1969) , and Rothen (1997) . Linage from wild-type K-12 to MG1655 was based on Bachmann (1996) and Hayashi et al. (2006) . Lineage from wild-type B to AC2516 was based on Boyer (1964) and Rothen (1997) . Conjugal transfer of genes is denoted by dashed arrows. AC2515 is a Salmonella typhosa strain carrying F- lac from E. coli ( Johnson et al., 1964 ). KLF4 is an F′ factor ( Low, 1968 ) that was derived from AB259, an Hfr (Hayes) strain. Note that rpoS (Am) and rph-1 mutations do not appear in Bachmann (1972 , 1996 ). AO and EMB indicate acridine orange and eosin methylene blue agar, respectively.
    Maxi Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Qiagen max extraction kit
    Pedigree of <t>Escherichia</t> coli <t>RR1</t> and other related strains . Newly introduced mutations are denoted after a plus sign. Lineage from wild-type E. coli K-12 to W2961 was based on Bachmann (1972 , 1996 ). Lineage from W2961 to HB101 was based on Boyer and Roulland-Dussoix (1969) , and Rothen (1997) . Linage from wild-type K-12 to MG1655 was based on Bachmann (1996) and Hayashi et al. (2006) . Lineage from wild-type B to AC2516 was based on Boyer (1964) and Rothen (1997) . Conjugal transfer of genes is denoted by dashed arrows. AC2515 is a Salmonella typhosa strain carrying F- lac from E. coli ( Johnson et al., 1964 ). KLF4 is an F′ factor ( Low, 1968 ) that was derived from AB259, an Hfr (Hayes) strain. Note that rpoS (Am) and rph-1 mutations do not appear in Bachmann (1972 , 1996 ). AO and EMB indicate acridine orange and eosin methylene blue agar, respectively.
    Max Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Bio-Rad nylon membrane
    Pedigree of <t>Escherichia</t> coli <t>RR1</t> and other related strains . Newly introduced mutations are denoted after a plus sign. Lineage from wild-type E. coli K-12 to W2961 was based on Bachmann (1972 , 1996 ). Lineage from W2961 to HB101 was based on Boyer and Roulland-Dussoix (1969) , and Rothen (1997) . Linage from wild-type K-12 to MG1655 was based on Bachmann (1996) and Hayashi et al. (2006) . Lineage from wild-type B to AC2516 was based on Boyer (1964) and Rothen (1997) . Conjugal transfer of genes is denoted by dashed arrows. AC2515 is a Salmonella typhosa strain carrying F- lac from E. coli ( Johnson et al., 1964 ). KLF4 is an F′ factor ( Low, 1968 ) that was derived from AB259, an Hfr (Hayes) strain. Note that rpoS (Am) and rph-1 mutations do not appear in Bachmann (1972 , 1996 ). AO and EMB indicate acridine orange and eosin methylene blue agar, respectively.
    Nylon Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 2350 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio-Rad zeta probe membrane
    Pedigree of <t>Escherichia</t> coli <t>RR1</t> and other related strains . Newly introduced mutations are denoted after a plus sign. Lineage from wild-type E. coli K-12 to W2961 was based on Bachmann (1972 , 1996 ). Lineage from W2961 to HB101 was based on Boyer and Roulland-Dussoix (1969) , and Rothen (1997) . Linage from wild-type K-12 to MG1655 was based on Bachmann (1996) and Hayashi et al. (2006) . Lineage from wild-type B to AC2516 was based on Boyer (1964) and Rothen (1997) . Conjugal transfer of genes is denoted by dashed arrows. AC2515 is a Salmonella typhosa strain carrying F- lac from E. coli ( Johnson et al., 1964 ). KLF4 is an F′ factor ( Low, 1968 ) that was derived from AB259, an Hfr (Hayes) strain. Note that rpoS (Am) and rph-1 mutations do not appear in Bachmann (1972 , 1996 ). AO and EMB indicate acridine orange and eosin methylene blue agar, respectively.
    Zeta Probe Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1651 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Bio-Rad agarose gel
    Pedigree of <t>Escherichia</t> coli <t>RR1</t> and other related strains . Newly introduced mutations are denoted after a plus sign. Lineage from wild-type E. coli K-12 to W2961 was based on Bachmann (1972 , 1996 ). Lineage from W2961 to HB101 was based on Boyer and Roulland-Dussoix (1969) , and Rothen (1997) . Linage from wild-type K-12 to MG1655 was based on Bachmann (1996) and Hayashi et al. (2006) . Lineage from wild-type B to AC2516 was based on Boyer (1964) and Rothen (1997) . Conjugal transfer of genes is denoted by dashed arrows. AC2515 is a Salmonella typhosa strain carrying F- lac from E. coli ( Johnson et al., 1964 ). KLF4 is an F′ factor ( Low, 1968 ) that was derived from AB259, an Hfr (Hayes) strain. Note that rpoS (Am) and rph-1 mutations do not appear in Bachmann (1972 , 1996 ). AO and EMB indicate acridine orange and eosin methylene blue agar, respectively.
    Agarose Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 5921 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher lennox lb
    Pedigree of <t>Escherichia</t> coli <t>RR1</t> and other related strains . Newly introduced mutations are denoted after a plus sign. Lineage from wild-type E. coli K-12 to W2961 was based on Bachmann (1972 , 1996 ). Lineage from W2961 to HB101 was based on Boyer and Roulland-Dussoix (1969) , and Rothen (1997) . Linage from wild-type K-12 to MG1655 was based on Bachmann (1996) and Hayashi et al. (2006) . Lineage from wild-type B to AC2516 was based on Boyer (1964) and Rothen (1997) . Conjugal transfer of genes is denoted by dashed arrows. AC2515 is a Salmonella typhosa strain carrying F- lac from E. coli ( Johnson et al., 1964 ). KLF4 is an F′ factor ( Low, 1968 ) that was derived from AB259, an Hfr (Hayes) strain. Note that rpoS (Am) and rph-1 mutations do not appear in Bachmann (1972 , 1996 ). AO and EMB indicate acridine orange and eosin methylene blue agar, respectively.
    Lennox Lb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Becton Dickinson vcam 1
    Flow cytometric analysis of the species- and strain-dependent expression of surface adhesion molecules on bacterium-infected EC. Monolayers of EC were incubated for 24 h with culture medium alone (no stimulus), with 5 ng of rIL-1/ ml (positive control), or with the indicated bacteria and then analyzed for surface expression of ICAM-1 and <t>VCAM-1</t> molecules. Data from one representative experiment are shown. Boxed numbers represent the mean fluorescence intensities expressed in arbitrary units of all analyzed EC. Mean background fluorescence, established by incubation of EC with the phycoerythrin-conjugated MAb only (PE-conjugate), ranged from 13 to 19 arbitrary units.
    Vcam 1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 688 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Endogen Inc mab cbr ic2 2
    Flow cytometric analysis of the species- and strain-dependent expression of surface adhesion molecules on bacterium-infected EC. Monolayers of EC were incubated for 24 h with culture medium alone (no stimulus), with 5 ng of rIL-1/ ml (positive control), or with the indicated bacteria and then analyzed for surface expression of ICAM-1 and <t>VCAM-1</t> molecules. Data from one representative experiment are shown. Boxed numbers represent the mean fluorescence intensities expressed in arbitrary units of all analyzed EC. Mean background fluorescence, established by incubation of EC with the phycoerythrin-conjugated MAb only (PE-conjugate), ranged from 13 to 19 arbitrary units.
    Mab Cbr Ic2 2, supplied by Endogen Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies mab 2lpm19c
    TF dependency of PCA of cocultures of monocytes and bacterium-infected EC. Monolayers of ≈2 × 10 5 S. aureus -, S. sanguis -, or S. epidermidis -infected EC or noninfected EC/well were cocultured for 6 h with 2.5 × 10 5 monocytes in normal or FVII-depleted human plasma (A) and in the absence (no MAb) or presence of 10 μg of anti-CD11b MAb <t>2LPM19C/ml.</t> Then, PCA was determined. Values are the means ± standard deviations for three (A) and six (B) separate experiments with EC from different donors.
    Mab 2lpm19c, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson mab l133 1
    TF dependency of PCA of cocultures of monocytes and bacterium-infected EC. Monolayers of ≈2 × 10 5 S. aureus -, S. sanguis -, or S. epidermidis -infected EC or noninfected EC/well were cocultured for 6 h with 2.5 × 10 5 monocytes in normal or FVII-depleted human plasma (A) and in the absence (no MAb) or presence of 10 μg of anti-CD11b MAb <t>2LPM19C/ml.</t> Then, PCA was determined. Values are the means ± standard deviations for three (A) and six (B) separate experiments with EC from different donors.
    Mab L133 1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson mab e1 6
    TF dependency of PCA of cocultures of monocytes and bacterium-infected EC. Monolayers of ≈2 × 10 5 S. aureus -, S. sanguis -, or S. epidermidis -infected EC or noninfected EC/well were cocultured for 6 h with 2.5 × 10 5 monocytes in normal or FVII-depleted human plasma (A) and in the absence (no MAb) or presence of 10 μg of anti-CD11b MAb <t>2LPM19C/ml.</t> Then, PCA was determined. Values are the means ± standard deviations for three (A) and six (B) separate experiments with EC from different donors.
    Mab E1 6, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MONOSAN monoclonal antibodies mabs
    TF dependency of PCA of cocultures of monocytes and bacterium-infected EC. Monolayers of ≈2 × 10 5 S. aureus -, S. sanguis -, or S. epidermidis -infected EC or noninfected EC/well were cocultured for 6 h with 2.5 × 10 5 monocytes in normal or FVII-depleted human plasma (A) and in the absence (no MAb) or presence of 10 μg of anti-CD11b MAb <t>2LPM19C/ml.</t> Then, PCA was determined. Values are the means ± standard deviations for three (A) and six (B) separate experiments with EC from different donors.
    Monoclonal Antibodies Mabs, supplied by MONOSAN, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MONOSAN mab ena 1
    TF dependency of PCA of cocultures of monocytes and bacterium-infected EC. Monolayers of ≈2 × 10 5 S. aureus -, S. sanguis -, or S. epidermidis -infected EC or noninfected EC/well were cocultured for 6 h with 2.5 × 10 5 monocytes in normal or FVII-depleted human plasma (A) and in the absence (no MAb) or presence of 10 μg of anti-CD11b MAb <t>2LPM19C/ml.</t> Then, PCA was determined. Values are the means ± standard deviations for three (A) and six (B) separate experiments with EC from different donors.
    Mab Ena 1, supplied by MONOSAN, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher e coli strain dh5α
    Evaluation of OmpT-mediated release and small-scale purification of displayed Affibody molecule. A . Flow-cytometric analysis for comparison of surface expression of recombinant proteins between p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli <t>DH5α.</t> B . SDS-PAGE showing IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Supernatants from non-induced samples, indicated with (−), were included as controls. C . Mass spectrum from ESI-MS analysis of IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Theoretical molecular weight of the OmpT-cleaved Z IgG -His 6 is 9712 Da. D . SPR-based biosensor analysis on the IMAC-purified Z IgG -His 6 . Response units (RU) on the y-axis and time on the x-axis. Representative sensorgrams from injection of Z IgG -His 6 at three different concentrations over human IgG immobilized on the chip surface. Injections were performed in duplicates.
    E Coli Strain Dh5α, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 918 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs δ mcrc mrr
    Evaluation of OmpT-mediated release and small-scale purification of displayed Affibody molecule. A . Flow-cytometric analysis for comparison of surface expression of recombinant proteins between p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli <t>DH5α.</t> B . SDS-PAGE showing IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Supernatants from non-induced samples, indicated with (−), were included as controls. C . Mass spectrum from ESI-MS analysis of IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Theoretical molecular weight of the OmpT-cleaved Z IgG -His 6 is 9712 Da. D . SPR-based biosensor analysis on the IMAC-purified Z IgG -His 6 . Response units (RU) on the y-axis and time on the x-axis. Representative sensorgrams from injection of Z IgG -His 6 at three different concentrations over human IgG immobilized on the chip surface. Injections were performed in duplicates.
    δ Mcrc Mrr, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore cytidine 5
    Evaluation of OmpT-mediated release and small-scale purification of displayed Affibody molecule. A . Flow-cytometric analysis for comparison of surface expression of recombinant proteins between p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli <t>DH5α.</t> B . SDS-PAGE showing IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Supernatants from non-induced samples, indicated with (−), were included as controls. C . Mass spectrum from ESI-MS analysis of IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Theoretical molecular weight of the OmpT-cleaved Z IgG -His 6 is 9712 Da. D . SPR-based biosensor analysis on the IMAC-purified Z IgG -His 6 . Response units (RU) on the y-axis and time on the x-axis. Representative sensorgrams from injection of Z IgG -His 6 at three different concentrations over human IgG immobilized on the chip surface. Injections were performed in duplicates.
    Cytidine 5, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore f3648
    Evaluation of OmpT-mediated release and small-scale purification of displayed Affibody molecule. A . Flow-cytometric analysis for comparison of surface expression of recombinant proteins between p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli <t>DH5α.</t> B . SDS-PAGE showing IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Supernatants from non-induced samples, indicated with (−), were included as controls. C . Mass spectrum from ESI-MS analysis of IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Theoretical molecular weight of the OmpT-cleaved Z IgG -His 6 is 9712 Da. D . SPR-based biosensor analysis on the IMAC-purified Z IgG -His 6 . Response units (RU) on the y-axis and time on the x-axis. Representative sensorgrams from injection of Z IgG -His 6 at three different concentrations over human IgG immobilized on the chip surface. Injections were performed in duplicates.
    F3648, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA e coli strain bl21 de3
    Evaluation of OmpT-mediated release and small-scale purification of displayed Affibody molecule. A . Flow-cytometric analysis for comparison of surface expression of recombinant proteins between p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli <t>DH5α.</t> B . SDS-PAGE showing IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Supernatants from non-induced samples, indicated with (−), were included as controls. C . Mass spectrum from ESI-MS analysis of IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Theoretical molecular weight of the OmpT-cleaved Z IgG -His 6 is 9712 Da. D . SPR-based biosensor analysis on the IMAC-purified Z IgG -His 6 . Response units (RU) on the y-axis and time on the x-axis. Representative sensorgrams from injection of Z IgG -His 6 at three different concentrations over human IgG immobilized on the chip surface. Injections were performed in duplicates.
    E Coli Strain Bl21 De3, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore shrna
    Evaluation of OmpT-mediated release and small-scale purification of displayed Affibody molecule. A . Flow-cytometric analysis for comparison of surface expression of recombinant proteins between p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli <t>DH5α.</t> B . SDS-PAGE showing IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Supernatants from non-induced samples, indicated with (−), were included as controls. C . Mass spectrum from ESI-MS analysis of IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Theoretical molecular weight of the OmpT-cleaved Z IgG -His 6 is 9712 Da. D . SPR-based biosensor analysis on the IMAC-purified Z IgG -His 6 . Response units (RU) on the y-axis and time on the x-axis. Representative sensorgrams from injection of Z IgG -His 6 at three different concentrations over human IgG immobilized on the chip surface. Injections were performed in duplicates.
    Shrna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1724 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad hybridization procedures
    Evaluation of OmpT-mediated release and small-scale purification of displayed Affibody molecule. A . Flow-cytometric analysis for comparison of surface expression of recombinant proteins between p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli <t>DH5α.</t> B . SDS-PAGE showing IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Supernatants from non-induced samples, indicated with (−), were included as controls. C . Mass spectrum from ESI-MS analysis of IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Theoretical molecular weight of the OmpT-cleaved Z IgG -His 6 is 9712 Da. D . SPR-based biosensor analysis on the IMAC-purified Z IgG -His 6 . Response units (RU) on the y-axis and time on the x-axis. Representative sensorgrams from injection of Z IgG -His 6 at three different concentrations over human IgG immobilized on the chip surface. Injections were performed in duplicates.
    Hybridization Procedures, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems mab against human tumor necrosis factor alpha tnf α
    Contribution of endogenous IL-1 or <t>TNF-α</t> to enhanced TFA of monocyte-EC cocultures. Monolayers of 2 × 10 5 EC/well were incubated for 24 h with culture medium alone (no bacteria) or with ∼4 × 10 7 cells of the indicated bacteria, washed, and incubated for about 15 min with culture medium with (+) or without (−) 1 μg of anti-human TNF-α MAb/ml and/or 30 ng of rIL-1ra/ml. Then, these cells were cocultured for 6 h with 2 × 10 5 monocytes in the absence (−) or presence (+) of anti-human TNF-α MAb and/or rIL-1ra. TFA was determined as described in Materials and Methods. Values are the means ± standard deviations for eight separate experiments with EC from different donors. P values were
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    Millipore bacterial lps
    Contribution of endogenous IL-1 or <t>TNF-α</t> to enhanced TFA of monocyte-EC cocultures. Monolayers of 2 × 10 5 EC/well were incubated for 24 h with culture medium alone (no bacteria) or with ∼4 × 10 7 cells of the indicated bacteria, washed, and incubated for about 15 min with culture medium with (+) or without (−) 1 μg of anti-human TNF-α MAb/ml and/or 30 ng of rIL-1ra/ml. Then, these cells were cocultured for 6 h with 2 × 10 5 monocytes in the absence (−) or presence (+) of anti-human TNF-α MAb and/or rIL-1ra. TFA was determined as described in Materials and Methods. Values are the means ± standard deviations for eight separate experiments with EC from different donors. P values were
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    Image Search Results


    Pedigree of Escherichia coli RR1 and other related strains . Newly introduced mutations are denoted after a plus sign. Lineage from wild-type E. coli K-12 to W2961 was based on Bachmann (1972 , 1996 ). Lineage from W2961 to HB101 was based on Boyer and Roulland-Dussoix (1969) , and Rothen (1997) . Linage from wild-type K-12 to MG1655 was based on Bachmann (1996) and Hayashi et al. (2006) . Lineage from wild-type B to AC2516 was based on Boyer (1964) and Rothen (1997) . Conjugal transfer of genes is denoted by dashed arrows. AC2515 is a Salmonella typhosa strain carrying F- lac from E. coli ( Johnson et al., 1964 ). KLF4 is an F′ factor ( Low, 1968 ) that was derived from AB259, an Hfr (Hayes) strain. Note that rpoS (Am) and rph-1 mutations do not appear in Bachmann (1972 , 1996 ). AO and EMB indicate acridine orange and eosin methylene blue agar, respectively.

    Journal: Frontiers in Microbiology

    Article Title: Unveiling the Hybrid Genome Structure of Escherichia coli RR1 (HB101 RecA+)

    doi: 10.3389/fmicb.2017.00585

    Figure Lengend Snippet: Pedigree of Escherichia coli RR1 and other related strains . Newly introduced mutations are denoted after a plus sign. Lineage from wild-type E. coli K-12 to W2961 was based on Bachmann (1972 , 1996 ). Lineage from W2961 to HB101 was based on Boyer and Roulland-Dussoix (1969) , and Rothen (1997) . Linage from wild-type K-12 to MG1655 was based on Bachmann (1996) and Hayashi et al. (2006) . Lineage from wild-type B to AC2516 was based on Boyer (1964) and Rothen (1997) . Conjugal transfer of genes is denoted by dashed arrows. AC2515 is a Salmonella typhosa strain carrying F- lac from E. coli ( Johnson et al., 1964 ). KLF4 is an F′ factor ( Low, 1968 ) that was derived from AB259, an Hfr (Hayes) strain. Note that rpoS (Am) and rph-1 mutations do not appear in Bachmann (1972 , 1996 ). AO and EMB indicate acridine orange and eosin methylene blue agar, respectively.

    Article Snippet: Bacterial Strains and Genome Sequencing Escherichia coli strain RR1 (= KCTC 2134 or ATCC 31343) for genome sequencing was purchased from the Korean Collection for Type Cultures (Jeongeup, Jeollabuk-do, South Korea).

    Techniques: Derivative Assay

    Flow cytometric analysis of the species- and strain-dependent expression of surface adhesion molecules on bacterium-infected EC. Monolayers of EC were incubated for 24 h with culture medium alone (no stimulus), with 5 ng of rIL-1/ ml (positive control), or with the indicated bacteria and then analyzed for surface expression of ICAM-1 and VCAM-1 molecules. Data from one representative experiment are shown. Boxed numbers represent the mean fluorescence intensities expressed in arbitrary units of all analyzed EC. Mean background fluorescence, established by incubation of EC with the phycoerythrin-conjugated MAb only (PE-conjugate), ranged from 13 to 19 arbitrary units.

    Journal: Infection and Immunity

    Article Title: Monocytes Augment Bacterial Species- and Strain-Dependent Induction of Tissue Factor Activity in Bacterium-Infected Human Vascular Endothelial Cells

    doi: 10.1128/IAI.69.5.2797-2807.2001

    Figure Lengend Snippet: Flow cytometric analysis of the species- and strain-dependent expression of surface adhesion molecules on bacterium-infected EC. Monolayers of EC were incubated for 24 h with culture medium alone (no stimulus), with 5 ng of rIL-1/ ml (positive control), or with the indicated bacteria and then analyzed for surface expression of ICAM-1 and VCAM-1 molecules. Data from one representative experiment are shown. Boxed numbers represent the mean fluorescence intensities expressed in arbitrary units of all analyzed EC. Mean background fluorescence, established by incubation of EC with the phycoerythrin-conjugated MAb only (PE-conjugate), ranged from 13 to 19 arbitrary units.

    Article Snippet: The following monoclonal antibodies (MAbs) against surface molecules on human EC or blood leukocytes were used: MAb ENA-1 (immunoglobulin G1 [IgG1]; Monosan, Uden, The Netherlands) against E-selectin (CD62E) ( ); MAb RUU-SP 2.17 (IgG1) against P-selectin (CD62P) , a gift from K. Nieuwenhuizen (Department of Hematology, University Hospital, Utrecht, The Netherlands); MAb RR1/1 (IgG1) against ICAM-1 (CD54) , kindly donated by T. A. Springer (Dana Farber Cancer Institute, Boston, Mass.); MAb CBR-IC2/2 (IgG2a; Endogen, Inc., Boston, Mass.) against ICAM-2 (CD102) ( ); MAb E1/6 (IgG1; Becton Dickinson Benelux S.A., Aalst, Belgium) against VCAM-1 (CD106) ( ); MAb L133.1 (IgG1; Becton Dickinson) against platelet-endothelial cell adhesion molecule-1 (PECAM-1; CD31) ( ); MAb IB4 (IgG2a) against CD18 (β2 -integrin) , obtained as a supernatant of the hybridoma cell line (American Type Culture Collection, Manassas, Va.); MAb 15A8 (IgG1; Central Laboratory for Blood Transfusion, Amsterdam, The Netherlands) against VLA-4α (CD49d) ( ); MAb 2LPM19C (IgG1; Dako, Glostrup, Denmark) against the I-domain of CR3α (CD11b) ( ); and TF9-10H10 (IgG1; OMNILabo International B.V., Breda, The Netherlands) against TF (CD142) ( ).

    Techniques: Flow Cytometry, Expressing, Infection, Incubation, Positive Control, Fluorescence

    TF dependency of PCA of cocultures of monocytes and bacterium-infected EC. Monolayers of ≈2 × 10 5 S. aureus -, S. sanguis -, or S. epidermidis -infected EC or noninfected EC/well were cocultured for 6 h with 2.5 × 10 5 monocytes in normal or FVII-depleted human plasma (A) and in the absence (no MAb) or presence of 10 μg of anti-CD11b MAb 2LPM19C/ml. Then, PCA was determined. Values are the means ± standard deviations for three (A) and six (B) separate experiments with EC from different donors.

    Journal: Infection and Immunity

    Article Title: Monocytes Augment Bacterial Species- and Strain-Dependent Induction of Tissue Factor Activity in Bacterium-Infected Human Vascular Endothelial Cells

    doi: 10.1128/IAI.69.5.2797-2807.2001

    Figure Lengend Snippet: TF dependency of PCA of cocultures of monocytes and bacterium-infected EC. Monolayers of ≈2 × 10 5 S. aureus -, S. sanguis -, or S. epidermidis -infected EC or noninfected EC/well were cocultured for 6 h with 2.5 × 10 5 monocytes in normal or FVII-depleted human plasma (A) and in the absence (no MAb) or presence of 10 μg of anti-CD11b MAb 2LPM19C/ml. Then, PCA was determined. Values are the means ± standard deviations for three (A) and six (B) separate experiments with EC from different donors.

    Article Snippet: The following monoclonal antibodies (MAbs) against surface molecules on human EC or blood leukocytes were used: MAb ENA-1 (immunoglobulin G1 [IgG1]; Monosan, Uden, The Netherlands) against E-selectin (CD62E) ( ); MAb RUU-SP 2.17 (IgG1) against P-selectin (CD62P) , a gift from K. Nieuwenhuizen (Department of Hematology, University Hospital, Utrecht, The Netherlands); MAb RR1/1 (IgG1) against ICAM-1 (CD54) , kindly donated by T. A. Springer (Dana Farber Cancer Institute, Boston, Mass.); MAb CBR-IC2/2 (IgG2a; Endogen, Inc., Boston, Mass.) against ICAM-2 (CD102) ( ); MAb E1/6 (IgG1; Becton Dickinson Benelux S.A., Aalst, Belgium) against VCAM-1 (CD106) ( ); MAb L133.1 (IgG1; Becton Dickinson) against platelet-endothelial cell adhesion molecule-1 (PECAM-1; CD31) ( ); MAb IB4 (IgG2a) against CD18 (β2 -integrin) , obtained as a supernatant of the hybridoma cell line (American Type Culture Collection, Manassas, Va.); MAb 15A8 (IgG1; Central Laboratory for Blood Transfusion, Amsterdam, The Netherlands) against VLA-4α (CD49d) ( ); MAb 2LPM19C (IgG1; Dako, Glostrup, Denmark) against the I-domain of CR3α (CD11b) ( ); and TF9-10H10 (IgG1; OMNILabo International B.V., Breda, The Netherlands) against TF (CD142) ( ).

    Techniques: Infection

    Evaluation of OmpT-mediated release and small-scale purification of displayed Affibody molecule. A . Flow-cytometric analysis for comparison of surface expression of recombinant proteins between p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. B . SDS-PAGE showing IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Supernatants from non-induced samples, indicated with (−), were included as controls. C . Mass spectrum from ESI-MS analysis of IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Theoretical molecular weight of the OmpT-cleaved Z IgG -His 6 is 9712 Da. D . SPR-based biosensor analysis on the IMAC-purified Z IgG -His 6 . Response units (RU) on the y-axis and time on the x-axis. Representative sensorgrams from injection of Z IgG -His 6 at three different concentrations over human IgG immobilized on the chip surface. Injections were performed in duplicates.

    Journal: Microbial Cell Factories

    Article Title: An engineered autotransporter-based surface expression vector enables efficient display of Affibody molecules on OmpT-negative E. coli as well as protease-mediated secretion in OmpT-positive strains

    doi: 10.1186/s12934-014-0179-z

    Figure Lengend Snippet: Evaluation of OmpT-mediated release and small-scale purification of displayed Affibody molecule. A . Flow-cytometric analysis for comparison of surface expression of recombinant proteins between p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. B . SDS-PAGE showing IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Supernatants from non-induced samples, indicated with (−), were included as controls. C . Mass spectrum from ESI-MS analysis of IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Theoretical molecular weight of the OmpT-cleaved Z IgG -His 6 is 9712 Da. D . SPR-based biosensor analysis on the IMAC-purified Z IgG -His 6 . Response units (RU) on the y-axis and time on the x-axis. Representative sensorgrams from injection of Z IgG -His 6 at three different concentrations over human IgG immobilized on the chip surface. Injections were performed in duplicates.

    Article Snippet: Bacterial strains Escherichia coli strain RR1∆M15 [ ] and E. coli strain DH5α (Invitrogen, Carlsbad, CA) were used for subcloning work.

    Techniques: Purification, Flow Cytometry, Expressing, Recombinant, SDS Page, Mass Spectrometry, Molecular Weight, SPR Assay, Injection, Chromatin Immunoprecipitation

    Contribution of endogenous IL-1 or TNF-α to enhanced TFA of monocyte-EC cocultures. Monolayers of 2 × 10 5 EC/well were incubated for 24 h with culture medium alone (no bacteria) or with ∼4 × 10 7 cells of the indicated bacteria, washed, and incubated for about 15 min with culture medium with (+) or without (−) 1 μg of anti-human TNF-α MAb/ml and/or 30 ng of rIL-1ra/ml. Then, these cells were cocultured for 6 h with 2 × 10 5 monocytes in the absence (−) or presence (+) of anti-human TNF-α MAb and/or rIL-1ra. TFA was determined as described in Materials and Methods. Values are the means ± standard deviations for eight separate experiments with EC from different donors. P values were

    Journal: Infection and Immunity

    Article Title: Monocytes Augment Bacterial Species- and Strain-Dependent Induction of Tissue Factor Activity in Bacterium-Infected Human Vascular Endothelial Cells

    doi: 10.1128/IAI.69.5.2797-2807.2001

    Figure Lengend Snippet: Contribution of endogenous IL-1 or TNF-α to enhanced TFA of monocyte-EC cocultures. Monolayers of 2 × 10 5 EC/well were incubated for 24 h with culture medium alone (no bacteria) or with ∼4 × 10 7 cells of the indicated bacteria, washed, and incubated for about 15 min with culture medium with (+) or without (−) 1 μg of anti-human TNF-α MAb/ml and/or 30 ng of rIL-1ra/ml. Then, these cells were cocultured for 6 h with 2 × 10 5 monocytes in the absence (−) or presence (+) of anti-human TNF-α MAb and/or rIL-1ra. TFA was determined as described in Materials and Methods. Values are the means ± standard deviations for eight separate experiments with EC from different donors. P values were

    Article Snippet: Neutralizing MAb against human tumor necrosis factor alpha (TNF-α) was purchased from R & D Systems.

    Techniques: Incubation