rq1 dnase Promega Search Results


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  • 99
    Promega rq1 promega dnase
    C. heterostrophus secretes DNases, and secretion is induced by plant tissue. WT, nuc1 mutant, nuc2 mutant, and nuc1 nuc2 double-mutant filtrates degrade intact λ DNA in the presence of plant material. The nuc1 single mutant and nuc1 nuc2 double mutant degrade lambda DNA less well than the WT or the nuc2 single mutant. This indicates that <t>DNase(s)</t> are secreted by the fungus, that the nuc1 mutant secretes a DNase that is important in DNA degradation, and that secretion is induced by host material. Left, size markers in kilobases. +, addition of corn leaf (CL) fragments, lambda DNA, or purified <t>RQ1</t> RNase-free DNase. Culture filtrates examined were from WT strain C4, nuc1 mutant strains 144206-3-1 and 8-1, nuc2 mutant strains 149183-2-1 and 3-1, and nuc1 nuc2 double-mutant strains 144206/149183-4-1 and 8-1. The negative-control reaction with λ DNA did not degrade the DNA, while the positive-control reaction with λ DNA plus DNase did. Also note that λ DNA was not degraded by the CL material.
    Rq1 Promega Dnase, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1799 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rq1 promega dnase/product/Promega
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    rq1 promega dnase - by Bioz Stars, 2020-05
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    85
    Promega promega s rq1 dnase
    C. heterostrophus secretes DNases, and secretion is induced by plant tissue. WT, nuc1 mutant, nuc2 mutant, and nuc1 nuc2 double-mutant filtrates degrade intact λ DNA in the presence of plant material. The nuc1 single mutant and nuc1 nuc2 double mutant degrade lambda DNA less well than the WT or the nuc2 single mutant. This indicates that <t>DNase(s)</t> are secreted by the fungus, that the nuc1 mutant secretes a DNase that is important in DNA degradation, and that secretion is induced by host material. Left, size markers in kilobases. +, addition of corn leaf (CL) fragments, lambda DNA, or purified <t>RQ1</t> RNase-free DNase. Culture filtrates examined were from WT strain C4, nuc1 mutant strains 144206-3-1 and 8-1, nuc2 mutant strains 149183-2-1 and 3-1, and nuc1 nuc2 double-mutant strains 144206/149183-4-1 and 8-1. The negative-control reaction with λ DNA did not degrade the DNA, while the positive-control reaction with λ DNA plus DNase did. Also note that λ DNA was not degraded by the CL material.
    Promega S Rq1 Dnase, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/promega s rq1 dnase/product/Promega
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    promega s rq1 dnase - by Bioz Stars, 2020-05
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    88
    Promega rq1 dnase kit
    C. heterostrophus secretes DNases, and secretion is induced by plant tissue. WT, nuc1 mutant, nuc2 mutant, and nuc1 nuc2 double-mutant filtrates degrade intact λ DNA in the presence of plant material. The nuc1 single mutant and nuc1 nuc2 double mutant degrade lambda DNA less well than the WT or the nuc2 single mutant. This indicates that <t>DNase(s)</t> are secreted by the fungus, that the nuc1 mutant secretes a DNase that is important in DNA degradation, and that secretion is induced by host material. Left, size markers in kilobases. +, addition of corn leaf (CL) fragments, lambda DNA, or purified <t>RQ1</t> RNase-free DNase. Culture filtrates examined were from WT strain C4, nuc1 mutant strains 144206-3-1 and 8-1, nuc2 mutant strains 149183-2-1 and 3-1, and nuc1 nuc2 double-mutant strains 144206/149183-4-1 and 8-1. The negative-control reaction with λ DNA did not degrade the DNA, while the positive-control reaction with λ DNA plus DNase did. Also note that λ DNA was not degraded by the CL material.
    Rq1 Dnase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rq1 dnase kit/product/Promega
    Average 88 stars, based on 78 article reviews
    Price from $9.99 to $1999.99
    rq1 dnase kit - by Bioz Stars, 2020-05
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    93
    Promega rq1 dnase buffer
    C. heterostrophus secretes DNases, and secretion is induced by plant tissue. WT, nuc1 mutant, nuc2 mutant, and nuc1 nuc2 double-mutant filtrates degrade intact λ DNA in the presence of plant material. The nuc1 single mutant and nuc1 nuc2 double mutant degrade lambda DNA less well than the WT or the nuc2 single mutant. This indicates that <t>DNase(s)</t> are secreted by the fungus, that the nuc1 mutant secretes a DNase that is important in DNA degradation, and that secretion is induced by host material. Left, size markers in kilobases. +, addition of corn leaf (CL) fragments, lambda DNA, or purified <t>RQ1</t> RNase-free DNase. Culture filtrates examined were from WT strain C4, nuc1 mutant strains 144206-3-1 and 8-1, nuc2 mutant strains 149183-2-1 and 3-1, and nuc1 nuc2 double-mutant strains 144206/149183-4-1 and 8-1. The negative-control reaction with λ DNA did not degrade the DNA, while the positive-control reaction with λ DNA plus DNase did. Also note that λ DNA was not degraded by the CL material.
    Rq1 Dnase Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rq1 dnase buffer/product/Promega
    Average 93 stars, based on 79 article reviews
    Price from $9.99 to $1999.99
    rq1 dnase buffer - by Bioz Stars, 2020-05
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    88
    Promega rq1 dnase enzyme
    C. heterostrophus secretes DNases, and secretion is induced by plant tissue. WT, nuc1 mutant, nuc2 mutant, and nuc1 nuc2 double-mutant filtrates degrade intact λ DNA in the presence of plant material. The nuc1 single mutant and nuc1 nuc2 double mutant degrade lambda DNA less well than the WT or the nuc2 single mutant. This indicates that <t>DNase(s)</t> are secreted by the fungus, that the nuc1 mutant secretes a DNase that is important in DNA degradation, and that secretion is induced by host material. Left, size markers in kilobases. +, addition of corn leaf (CL) fragments, lambda DNA, or purified <t>RQ1</t> RNase-free DNase. Culture filtrates examined were from WT strain C4, nuc1 mutant strains 144206-3-1 and 8-1, nuc2 mutant strains 149183-2-1 and 3-1, and nuc1 nuc2 double-mutant strains 144206/149183-4-1 and 8-1. The negative-control reaction with λ DNA did not degrade the DNA, while the positive-control reaction with λ DNA plus DNase did. Also note that λ DNA was not degraded by the CL material.
    Rq1 Dnase Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rq1 dnase enzyme/product/Promega
    Average 88 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    rq1 dnase enzyme - by Bioz Stars, 2020-05
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    85
    Promega rq1 dnase 1
    C. heterostrophus secretes DNases, and secretion is induced by plant tissue. WT, nuc1 mutant, nuc2 mutant, and nuc1 nuc2 double-mutant filtrates degrade intact λ DNA in the presence of plant material. The nuc1 single mutant and nuc1 nuc2 double mutant degrade lambda DNA less well than the WT or the nuc2 single mutant. This indicates that <t>DNase(s)</t> are secreted by the fungus, that the nuc1 mutant secretes a DNase that is important in DNA degradation, and that secretion is induced by host material. Left, size markers in kilobases. +, addition of corn leaf (CL) fragments, lambda DNA, or purified <t>RQ1</t> RNase-free DNase. Culture filtrates examined were from WT strain C4, nuc1 mutant strains 144206-3-1 and 8-1, nuc2 mutant strains 149183-2-1 and 3-1, and nuc1 nuc2 double-mutant strains 144206/149183-4-1 and 8-1. The negative-control reaction with λ DNA did not degrade the DNA, while the positive-control reaction with λ DNA plus DNase did. Also note that λ DNA was not degraded by the CL material.
    Rq1 Dnase 1, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rq1 dnase 1/product/Promega
    Average 85 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    rq1 dnase 1 - by Bioz Stars, 2020-05
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    93
    Promega rq1 rnaase free dnaase kit
    C. heterostrophus secretes DNases, and secretion is induced by plant tissue. WT, nuc1 mutant, nuc2 mutant, and nuc1 nuc2 double-mutant filtrates degrade intact λ DNA in the presence of plant material. The nuc1 single mutant and nuc1 nuc2 double mutant degrade lambda DNA less well than the WT or the nuc2 single mutant. This indicates that <t>DNase(s)</t> are secreted by the fungus, that the nuc1 mutant secretes a DNase that is important in DNA degradation, and that secretion is induced by host material. Left, size markers in kilobases. +, addition of corn leaf (CL) fragments, lambda DNA, or purified <t>RQ1</t> RNase-free DNase. Culture filtrates examined were from WT strain C4, nuc1 mutant strains 144206-3-1 and 8-1, nuc2 mutant strains 149183-2-1 and 3-1, and nuc1 nuc2 double-mutant strains 144206/149183-4-1 and 8-1. The negative-control reaction with λ DNA did not degrade the DNA, while the positive-control reaction with λ DNA plus DNase did. Also note that λ DNA was not degraded by the CL material.
    Rq1 Rnaase Free Dnaase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rq1 rnaase free dnaase kit/product/Promega
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    rq1 rnaase free dnaase kit - by Bioz Stars, 2020-05
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    Image Search Results


    C. heterostrophus secretes DNases, and secretion is induced by plant tissue. WT, nuc1 mutant, nuc2 mutant, and nuc1 nuc2 double-mutant filtrates degrade intact λ DNA in the presence of plant material. The nuc1 single mutant and nuc1 nuc2 double mutant degrade lambda DNA less well than the WT or the nuc2 single mutant. This indicates that DNase(s) are secreted by the fungus, that the nuc1 mutant secretes a DNase that is important in DNA degradation, and that secretion is induced by host material. Left, size markers in kilobases. +, addition of corn leaf (CL) fragments, lambda DNA, or purified RQ1 RNase-free DNase. Culture filtrates examined were from WT strain C4, nuc1 mutant strains 144206-3-1 and 8-1, nuc2 mutant strains 149183-2-1 and 3-1, and nuc1 nuc2 double-mutant strains 144206/149183-4-1 and 8-1. The negative-control reaction with λ DNA did not degrade the DNA, while the positive-control reaction with λ DNA plus DNase did. Also note that λ DNA was not degraded by the CL material.

    Journal: mBio

    Article Title: A DNase from a Fungal Phytopathogen Is a Virulence Factor Likely Deployed as Counter Defense against Host-Secreted Extracellular DNA

    doi: 10.1128/mBio.02805-18

    Figure Lengend Snippet: C. heterostrophus secretes DNases, and secretion is induced by plant tissue. WT, nuc1 mutant, nuc2 mutant, and nuc1 nuc2 double-mutant filtrates degrade intact λ DNA in the presence of plant material. The nuc1 single mutant and nuc1 nuc2 double mutant degrade lambda DNA less well than the WT or the nuc2 single mutant. This indicates that DNase(s) are secreted by the fungus, that the nuc1 mutant secretes a DNase that is important in DNA degradation, and that secretion is induced by host material. Left, size markers in kilobases. +, addition of corn leaf (CL) fragments, lambda DNA, or purified RQ1 RNase-free DNase. Culture filtrates examined were from WT strain C4, nuc1 mutant strains 144206-3-1 and 8-1, nuc2 mutant strains 149183-2-1 and 3-1, and nuc1 nuc2 double-mutant strains 144206/149183-4-1 and 8-1. The negative-control reaction with λ DNA did not degrade the DNA, while the positive-control reaction with λ DNA plus DNase did. Also note that λ DNA was not degraded by the CL material.

    Article Snippet: Reactions were run on 1% agarose gels at 100 V for 15 min. As a positive control for λ DNA degradation, 1 unit of RQ1 DNase (catalog no. M610A; Promega) was used.

    Techniques: Mutagenesis, Lambda DNA Preparation, Purification, Negative Control, Positive Control

    Discrete radiolabelled RNA bands are detected after incubation of the axolotl and Xenopus extracts in the presence of [α– 32 P] CTP or [α– 32 P] UTP. Polymerization reactions were performed using axolotl or Xenopus LSE with either [α– 32 P] CTP or [α– 32 P] UTP (lane 4) and in the presence (lane 6) or not of 200 µg/mL α-amanitine, an RNA pol II and pol III inhibitor. After the indicated incubation time, samples were treated at 37°C during 1 hr with RNase A (lane 5) or during 15 min with RQ1 DNase (lane 8) or not (lanes 1–4, 6, 7, 9–11). RNA was extracted, electrophorezed through a denaturating agarose gel and visualized after ethidium bromide staining and UV illumination; the gel was dried and autoradiographied. Migration lengths of RNA size markers are indicated in bases (b).

    Journal: PLoS ONE

    Article Title: Evidence for an RNA Polymerization Activity in Axolotl and Xenopus Egg Extracts

    doi: 10.1371/journal.pone.0014411

    Figure Lengend Snippet: Discrete radiolabelled RNA bands are detected after incubation of the axolotl and Xenopus extracts in the presence of [α– 32 P] CTP or [α– 32 P] UTP. Polymerization reactions were performed using axolotl or Xenopus LSE with either [α– 32 P] CTP or [α– 32 P] UTP (lane 4) and in the presence (lane 6) or not of 200 µg/mL α-amanitine, an RNA pol II and pol III inhibitor. After the indicated incubation time, samples were treated at 37°C during 1 hr with RNase A (lane 5) or during 15 min with RQ1 DNase (lane 8) or not (lanes 1–4, 6, 7, 9–11). RNA was extracted, electrophorezed through a denaturating agarose gel and visualized after ethidium bromide staining and UV illumination; the gel was dried and autoradiographied. Migration lengths of RNA size markers are indicated in bases (b).

    Article Snippet: RNase A (DNase and protease free) was purchased from Boehringer Mannheim, DNase RQ1 from Promega, α-amanitin, cordycepin and cordycepin 5′-triphosphate from Sigma and RiboRuler RNA ladders from Fermentas.

    Techniques: Incubation, Agarose Gel Electrophoresis, Staining, Migration

    Polymerase sub-complexes lacking Rpb1 accumulate in not5Δ . A and B . Total extracts from cells expressing the indicated Tap-tagged (TT) polymerase subunits were separated on native gels (upper panels) or SDS-PAGE (lower panels) and analyzed by western blotting with anti- CBP antibodies. C . Rpb9-TT was purified by single step affinity and the purified proteins were analyzed on native gels (upper panels) or SDS-PAGE (lower panels) and western blotting with anti-CBP antibodies (left panel) or anti-Rpb1 antibodies (right panel). D . Total extracts from cells expressing Rpb11-TT were either untreated (-) or treated with DNase or RNase as indicated and separated by Native-PAGE, and analyzed by western blotting with PAP antibodies.

    Journal: PLoS Genetics

    Article Title: The Not5 Subunit of the Ccr4-Not Complex Connects Transcription and Translation

    doi: 10.1371/journal.pgen.1004569

    Figure Lengend Snippet: Polymerase sub-complexes lacking Rpb1 accumulate in not5Δ . A and B . Total extracts from cells expressing the indicated Tap-tagged (TT) polymerase subunits were separated on native gels (upper panels) or SDS-PAGE (lower panels) and analyzed by western blotting with anti- CBP antibodies. C . Rpb9-TT was purified by single step affinity and the purified proteins were analyzed on native gels (upper panels) or SDS-PAGE (lower panels) and western blotting with anti-CBP antibodies (left panel) or anti-Rpb1 antibodies (right panel). D . Total extracts from cells expressing Rpb11-TT were either untreated (-) or treated with DNase or RNase as indicated and separated by Native-PAGE, and analyzed by western blotting with PAP antibodies.

    Article Snippet: Pellets were resuspended in H2 O and were DNaseI treated (RQ1 RNase-free DNase, Promega).

    Techniques: Expressing, SDS Page, Western Blot, Purification, Clear Native PAGE

    Measurement of PrP expression in the developing murine embryo by RT-PCR. First-strand cDNA synthesis was performed on 5 μg of DNase-treated total RNA extracted from murine embryos at E9, E11, E13, E15, and E17 stages of development [with (+) or without (−) RT]. A control in which template cDNA was omitted was also included (N). (a) Ethidium bromide-stained agarose gel following electrophoresis of RT-PCR products of exon 3 of the Prnp gene. (b) Southern analysis of gel depicted in (a), probed with a radiolabeled 930-bp KpnI-Eco RI PrP DNA. (c) Ethidium bromide-stained agarose gel following electrophoresis of RT-PCR products of β-actin.

    Journal: Gene Expression

    Article Title: Embryonic Activation and Developmental Expression of the Murine Prion Protein Gene

    doi:

    Figure Lengend Snippet: Measurement of PrP expression in the developing murine embryo by RT-PCR. First-strand cDNA synthesis was performed on 5 μg of DNase-treated total RNA extracted from murine embryos at E9, E11, E13, E15, and E17 stages of development [with (+) or without (−) RT]. A control in which template cDNA was omitted was also included (N). (a) Ethidium bromide-stained agarose gel following electrophoresis of RT-PCR products of exon 3 of the Prnp gene. (b) Southern analysis of gel depicted in (a), probed with a radiolabeled 930-bp KpnI-Eco RI PrP DNA. (c) Ethidium bromide-stained agarose gel following electrophoresis of RT-PCR products of β-actin.

    Article Snippet: First-strand cDNA was prepared using a cDNA Synthesis Kit and a Not I-d(T)18 primer according to the manufacturer’s instructions (Amersham Pharmacia Biotech, Bucks, UK) and 5 μg of total RNA (prepared from embryos pooled from several pregnancies) pretreated with DNase (RQ1 RNase-free DNase; Promega, Southampton, UK).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Staining, Agarose Gel Electrophoresis, Electrophoresis

    Quality of root-extracted RNA. The analysis of the total RNA extracted from inoculated roots was performed using an Agilent Bioanalyser 2100. One microlitre of RQ1 DNase-treated total RNA was analysed for each sample. The days post-inoculation with Gaeumannomyces graminis var. tritici ( Ggt ) are indicated. M, RNA size marker; lanes 1, 3, 6, 9, RNA from uninfected wheat roots; lanes 2, 4, 7, 10, RNA from wheat roots infected with Ggt ; lanes 5, 8, 11, RNA from wheat roots infected with Ggt and Pf29Arp; lane 12, RNA from soil-borne pathogenic fungus IV-26/00.

    Journal: Molecular Plant Pathology

    Article Title: The biocontrol bacterium Pseudomonas fluorescens Pf29Arp strain affects the pathogenesis-related gene expression of the take-all fungus Gaeumannomyces graminis var. tritici on wheat roots

    doi: 10.1111/j.1364-3703.2011.00715.x

    Figure Lengend Snippet: Quality of root-extracted RNA. The analysis of the total RNA extracted from inoculated roots was performed using an Agilent Bioanalyser 2100. One microlitre of RQ1 DNase-treated total RNA was analysed for each sample. The days post-inoculation with Gaeumannomyces graminis var. tritici ( Ggt ) are indicated. M, RNA size marker; lanes 1, 3, 6, 9, RNA from uninfected wheat roots; lanes 2, 4, 7, 10, RNA from wheat roots infected with Ggt ; lanes 5, 8, 11, RNA from wheat roots infected with Ggt and Pf29Arp; lane 12, RNA from soil-borne pathogenic fungus IV-26/00.

    Article Snippet: Total RNA was extracted from ground tissue in 1 mL of Trizol (Invitrogen, Paisley, Renfrewshire, UK), and contaminating DNA was removed using the RNase-free RQ1 DNase (Promega Corp., Madison, WI, USA) according to the manufacturer's instructions.

    Techniques: Marker, Infection