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  • 99
    Thermo Fisher rq dnase
    Rq Dnase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rq dnase/product/Thermo Fisher
    Average 99 stars, based on 11 article reviews
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    rq dnase - by Bioz Stars, 2020-09
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    91
    Promega rq dnase i
    The E protein is essential for PRRSV replication. (A) Immunofluorescence of N protein in P129-ΔE inoculated cells. The ‘passage-1’ virus was prepared as culture supernatant harvested from BHK-21 cells transfected with P129-WT or P129-ΔE clone. Marc-145 cells were inoculated with ‘passage-1’ and fixed at the indicated times post-inoculation, followed by staining with N-specific MAb. (B) Detection of viral RNA in culture supernatants and in Marc-145 cells inoculated with ‘passage-1’ or ‘passage-2’ virus. Total RNA was extracted and treated with <t>DNase</t> I followed by RT-PCR for E gene. (C) Strand-specific detection of viral RNA in cells by RT-PCR. Marc-145 cells were inoculated with ‘passage-1’ P129-WT or ‘passage-1’ P129-ΔE, and total cellular RNA was extracted at 2 days post-inoculation. The RNA was treated by DNase I and RT-PCR was conducted to amplify the region as illustrated in the figure. Numbers in parenthesis indicate the 5′ most nucleotide position in each primer with respect to the PRRSV genome. Expected sizes of amplified products are indicated on the right of the gel.
    Rq Dnase I, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 432 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rq dnase i/product/Promega
    Average 91 stars, based on 432 article reviews
    Price from $9.99 to $1999.99
    rq dnase i - by Bioz Stars, 2020-09
    91/100 stars
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    88
    Promega rq rnase free dnase i
    The E protein is essential for PRRSV replication. (A) Immunofluorescence of N protein in P129-ΔE inoculated cells. The ‘passage-1’ virus was prepared as culture supernatant harvested from BHK-21 cells transfected with P129-WT or P129-ΔE clone. Marc-145 cells were inoculated with ‘passage-1’ and fixed at the indicated times post-inoculation, followed by staining with N-specific MAb. (B) Detection of viral RNA in culture supernatants and in Marc-145 cells inoculated with ‘passage-1’ or ‘passage-2’ virus. Total RNA was extracted and treated with <t>DNase</t> I followed by RT-PCR for E gene. (C) Strand-specific detection of viral RNA in cells by RT-PCR. Marc-145 cells were inoculated with ‘passage-1’ P129-WT or ‘passage-1’ P129-ΔE, and total cellular RNA was extracted at 2 days post-inoculation. The RNA was treated by DNase I and RT-PCR was conducted to amplify the region as illustrated in the figure. Numbers in parenthesis indicate the 5′ most nucleotide position in each primer with respect to the PRRSV genome. Expected sizes of amplified products are indicated on the right of the gel.
    Rq Rnase Free Dnase I, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rq rnase free dnase i/product/Promega
    Average 88 stars, based on 85 article reviews
    Price from $9.99 to $1999.99
    rq rnase free dnase i - by Bioz Stars, 2020-09
    88/100 stars
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    99
    Promega rq 1 rnase free dnase i
    The E protein is essential for PRRSV replication. (A) Immunofluorescence of N protein in P129-ΔE inoculated cells. The ‘passage-1’ virus was prepared as culture supernatant harvested from BHK-21 cells transfected with P129-WT or P129-ΔE clone. Marc-145 cells were inoculated with ‘passage-1’ and fixed at the indicated times post-inoculation, followed by staining with N-specific MAb. (B) Detection of viral RNA in culture supernatants and in Marc-145 cells inoculated with ‘passage-1’ or ‘passage-2’ virus. Total RNA was extracted and treated with <t>DNase</t> I followed by RT-PCR for E gene. (C) Strand-specific detection of viral RNA in cells by RT-PCR. Marc-145 cells were inoculated with ‘passage-1’ P129-WT or ‘passage-1’ P129-ΔE, and total cellular RNA was extracted at 2 days post-inoculation. The RNA was treated by DNase I and RT-PCR was conducted to amplify the region as illustrated in the figure. Numbers in parenthesis indicate the 5′ most nucleotide position in each primer with respect to the PRRSV genome. Expected sizes of amplified products are indicated on the right of the gel.
    Rq 1 Rnase Free Dnase I, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 624 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rq 1 rnase free dnase i/product/Promega
    Average 99 stars, based on 624 article reviews
    Price from $9.99 to $1999.99
    rq 1 rnase free dnase i - by Bioz Stars, 2020-09
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    88
    Promega rq 1 dnaase i
    The E protein is essential for PRRSV replication. (A) Immunofluorescence of N protein in P129-ΔE inoculated cells. The ‘passage-1’ virus was prepared as culture supernatant harvested from BHK-21 cells transfected with P129-WT or P129-ΔE clone. Marc-145 cells were inoculated with ‘passage-1’ and fixed at the indicated times post-inoculation, followed by staining with N-specific MAb. (B) Detection of viral RNA in culture supernatants and in Marc-145 cells inoculated with ‘passage-1’ or ‘passage-2’ virus. Total RNA was extracted and treated with <t>DNase</t> I followed by RT-PCR for E gene. (C) Strand-specific detection of viral RNA in cells by RT-PCR. Marc-145 cells were inoculated with ‘passage-1’ P129-WT or ‘passage-1’ P129-ΔE, and total cellular RNA was extracted at 2 days post-inoculation. The RNA was treated by DNase I and RT-PCR was conducted to amplify the region as illustrated in the figure. Numbers in parenthesis indicate the 5′ most nucleotide position in each primer with respect to the PRRSV genome. Expected sizes of amplified products are indicated on the right of the gel.
    Rq 1 Dnaase I, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rq 1 dnaase i/product/Promega
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    rq 1 dnaase i - by Bioz Stars, 2020-09
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    Image Search Results


    The E protein is essential for PRRSV replication. (A) Immunofluorescence of N protein in P129-ΔE inoculated cells. The ‘passage-1’ virus was prepared as culture supernatant harvested from BHK-21 cells transfected with P129-WT or P129-ΔE clone. Marc-145 cells were inoculated with ‘passage-1’ and fixed at the indicated times post-inoculation, followed by staining with N-specific MAb. (B) Detection of viral RNA in culture supernatants and in Marc-145 cells inoculated with ‘passage-1’ or ‘passage-2’ virus. Total RNA was extracted and treated with DNase I followed by RT-PCR for E gene. (C) Strand-specific detection of viral RNA in cells by RT-PCR. Marc-145 cells were inoculated with ‘passage-1’ P129-WT or ‘passage-1’ P129-ΔE, and total cellular RNA was extracted at 2 days post-inoculation. The RNA was treated by DNase I and RT-PCR was conducted to amplify the region as illustrated in the figure. Numbers in parenthesis indicate the 5′ most nucleotide position in each primer with respect to the PRRSV genome. Expected sizes of amplified products are indicated on the right of the gel.

    Journal: Virology

    Article Title: The small envelope protein of porcine reproductive and respiratory syndrome virus possesses ion channel protein-like properties

    doi: 10.1016/j.virol.2006.07.013

    Figure Lengend Snippet: The E protein is essential for PRRSV replication. (A) Immunofluorescence of N protein in P129-ΔE inoculated cells. The ‘passage-1’ virus was prepared as culture supernatant harvested from BHK-21 cells transfected with P129-WT or P129-ΔE clone. Marc-145 cells were inoculated with ‘passage-1’ and fixed at the indicated times post-inoculation, followed by staining with N-specific MAb. (B) Detection of viral RNA in culture supernatants and in Marc-145 cells inoculated with ‘passage-1’ or ‘passage-2’ virus. Total RNA was extracted and treated with DNase I followed by RT-PCR for E gene. (C) Strand-specific detection of viral RNA in cells by RT-PCR. Marc-145 cells were inoculated with ‘passage-1’ P129-WT or ‘passage-1’ P129-ΔE, and total cellular RNA was extracted at 2 days post-inoculation. The RNA was treated by DNase I and RT-PCR was conducted to amplify the region as illustrated in the figure. Numbers in parenthesis indicate the 5′ most nucleotide position in each primer with respect to the PRRSV genome. Expected sizes of amplified products are indicated on the right of the gel.

    Article Snippet: Seventy microliters of each sample suspension were added with 2 μl of RQ DNase I (1 U/μl; Promega) and 3.6 μl of RNase A (10 mg/ml; Sigma), and the mixture was incubated for 1 h at 37 °C in the presence or absence of detergents (1 μl of Triton X-100, and 3.5 μl of 10% SDS).

    Techniques: Immunofluorescence, Transfection, Staining, Reverse Transcription Polymerase Chain Reaction, Amplification