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  • 99
    Thermo Fisher rpmi 1640medium
    Construction of S. oralis spxB deletion mutant. (A) Black arrow indicates the gene encoding pyruvate oxidase (SMSK23_0092 spxB). A targeted deletion mutant lacking this region was constructed by allelic exchange using the temperature-sensitive shuttle vector pSET4s. (B) S. oralis ATCC35037 wild-type (WT), spxB-deletion mutant (KO), or reverse mutant (Rev) was cultured in BHI broth or 5% <t>RPMI1640</t> medium at 37°C for 18 h in a 5% CO 2 atmosphere. Concentrations of H 2 O 2 in culture supernatants were quantitatively determined using a hydrogen peroxide colorimetric detection kit. Data are shown as the mean ± SD of triplicate samples. *p
    Rpmi 1640medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 240 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi 1640medium/product/Thermo Fisher
    Average 99 stars, based on 240 article reviews
    Price from $9.99 to $1999.99
    rpmi 1640medium - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher medium rpmi1640
    Construction of S. oralis spxB deletion mutant. (A) Black arrow indicates the gene encoding pyruvate oxidase (SMSK23_0092 spxB). A targeted deletion mutant lacking this region was constructed by allelic exchange using the temperature-sensitive shuttle vector pSET4s. (B) S. oralis ATCC35037 wild-type (WT), spxB-deletion mutant (KO), or reverse mutant (Rev) was cultured in BHI broth or 5% <t>RPMI1640</t> medium at 37°C for 18 h in a 5% CO 2 atmosphere. Concentrations of H 2 O 2 in culture supernatants were quantitatively determined using a hydrogen peroxide colorimetric detection kit. Data are shown as the mean ± SD of triplicate samples. *p
    Medium Rpmi1640, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/medium rpmi1640/product/Thermo Fisher
    Average 99 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    medium rpmi1640 - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Construction of S. oralis spxB deletion mutant. (A) Black arrow indicates the gene encoding pyruvate oxidase (SMSK23_0092 spxB). A targeted deletion mutant lacking this region was constructed by allelic exchange using the temperature-sensitive shuttle vector pSET4s. (B) S. oralis ATCC35037 wild-type (WT), spxB-deletion mutant (KO), or reverse mutant (Rev) was cultured in BHI broth or 5% RPMI1640 medium at 37°C for 18 h in a 5% CO 2 atmosphere. Concentrations of H 2 O 2 in culture supernatants were quantitatively determined using a hydrogen peroxide colorimetric detection kit. Data are shown as the mean ± SD of triplicate samples. *p

    Journal: PLoS ONE

    Article Title: Hydrogen Peroxide Produced by Oral Streptococci Induces Macrophage Cell Death

    doi: 10.1371/journal.pone.0062563

    Figure Lengend Snippet: Construction of S. oralis spxB deletion mutant. (A) Black arrow indicates the gene encoding pyruvate oxidase (SMSK23_0092 spxB). A targeted deletion mutant lacking this region was constructed by allelic exchange using the temperature-sensitive shuttle vector pSET4s. (B) S. oralis ATCC35037 wild-type (WT), spxB-deletion mutant (KO), or reverse mutant (Rev) was cultured in BHI broth or 5% RPMI1640 medium at 37°C for 18 h in a 5% CO 2 atmosphere. Concentrations of H 2 O 2 in culture supernatants were quantitatively determined using a hydrogen peroxide colorimetric detection kit. Data are shown as the mean ± SD of triplicate samples. *p

    Article Snippet: Cell culture The human monocyte cell line THP-1 cells were purchased from RIKEN Bioresource Center and cultured in RPMI1640 medium (Invitrogen, Carlsbad, CA, USA) supplemented with 5% fetal bovine serum (FBS) (Invitrogen) (5% FBS RPMI1640), penicillin (100 U/ml), and streptomycin (100 µg/ml) at 37°C in a 5% CO2 atmosphere.

    Techniques: Mutagenesis, Construct, Plasmid Preparation, Cell Culture