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  • 99
    ATCC rpmi1640
    Rpmi1640, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi1640/product/ATCC
    Average 99 stars, based on 80 article reviews
    Price from $9.99 to $1999.99
    rpmi1640 - by Bioz Stars, 2020-08
    99/100 stars
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    99
    Thermo Fisher rpmi1640 basic
    Construction of S. oralis spxB deletion mutant. (A) Black arrow indicates the gene encoding pyruvate oxidase (SMSK23_0092 spxB). A targeted deletion mutant lacking this region was constructed by allelic exchange using the temperature-sensitive shuttle vector pSET4s. (B) S. oralis ATCC35037 wild-type (WT), spxB-deletion mutant (KO), or reverse mutant (Rev) was cultured in BHI broth or 5% <t>RPMI1640</t> medium at 37°C for 18 h in a 5% CO 2 atmosphere. Concentrations of H 2 O 2 in culture supernatants were quantitatively determined using a hydrogen peroxide colorimetric detection kit. Data are shown as the mean ± SD of triplicate samples. *p
    Rpmi1640 Basic, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5459 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi1640 basic/product/Thermo Fisher
    Average 99 stars, based on 5459 article reviews
    Price from $9.99 to $1999.99
    rpmi1640 basic - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher rpmi1640 media
    Construction of S. oralis spxB deletion mutant. (A) Black arrow indicates the gene encoding pyruvate oxidase (SMSK23_0092 spxB). A targeted deletion mutant lacking this region was constructed by allelic exchange using the temperature-sensitive shuttle vector pSET4s. (B) S. oralis ATCC35037 wild-type (WT), spxB-deletion mutant (KO), or reverse mutant (Rev) was cultured in BHI broth or 5% <t>RPMI1640</t> medium at 37°C for 18 h in a 5% CO 2 atmosphere. Concentrations of H 2 O 2 in culture supernatants were quantitatively determined using a hydrogen peroxide colorimetric detection kit. Data are shown as the mean ± SD of triplicate samples. *p
    Rpmi1640 Media, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 787 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi1640 media/product/Thermo Fisher
    Average 99 stars, based on 787 article reviews
    Price from $9.99 to $1999.99
    rpmi1640 media - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Millipore rpmi1640
    Foam cell formation from macrophages by PLA2G3, PLA2G5 and PLA2G10. A , LDL (2 mg/ml) was incubated with or without recombinant human PLA2G3 (sPLA 2 -III) and PLA2G5 (sPLA 2 -V) for 24 h at 37 °C. Then the NEFA released was quantified ( n = 2). B and C , J774 cells were cultured for 24 h in the presence or absence of LDL (200 μg/ml) that had been incubated for 24 h with or without PLA2G3 or PLA2G5. Then the cells were homogenized, and cholesteryl ester contents were quantified ( n = 2) ( B ). Replicate cells were fixed and stained with oil red O ( C ). Ac-LDL (50 μg/ml) was used as a positive control for foam cell formation. Magnification was ×200. D , peritoneal macrophages obtained from PLA2G3 Tg ( III-Tg ) mice and PLA2G10 Tg ( X-Tg ) mice as well as WT mice were placed in <t>RPMI1640</t> containing 10% lipoprotein-deficient fetal bovine serum and were incubated with or without 200 μg/ml LDL or 50 μg/ml Ac-LDL. After 24 h, the cells were stained with oil red O (magnification, ×200). Magnified views of individual macrophages are shown in the inset. E , PGE 2 production by macrophages from PLA2G3 Tg ( left ) and PLA2G10 Tg ( right ) mice in comparison with WT mice; macrophages were incubated for 24 h in the presence (+) or absence (-) of 200 μg/ml LDL (mean ± S.E., n = 3–5; * , p
    Rpmi1640, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1998 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi1640/product/Millipore
    Average 99 stars, based on 1998 article reviews
    Price from $9.99 to $1999.99
    rpmi1640 - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Construction of S. oralis spxB deletion mutant. (A) Black arrow indicates the gene encoding pyruvate oxidase (SMSK23_0092 spxB). A targeted deletion mutant lacking this region was constructed by allelic exchange using the temperature-sensitive shuttle vector pSET4s. (B) S. oralis ATCC35037 wild-type (WT), spxB-deletion mutant (KO), or reverse mutant (Rev) was cultured in BHI broth or 5% RPMI1640 medium at 37°C for 18 h in a 5% CO 2 atmosphere. Concentrations of H 2 O 2 in culture supernatants were quantitatively determined using a hydrogen peroxide colorimetric detection kit. Data are shown as the mean ± SD of triplicate samples. *p

    Journal: PLoS ONE

    Article Title: Hydrogen Peroxide Produced by Oral Streptococci Induces Macrophage Cell Death

    doi: 10.1371/journal.pone.0062563

    Figure Lengend Snippet: Construction of S. oralis spxB deletion mutant. (A) Black arrow indicates the gene encoding pyruvate oxidase (SMSK23_0092 spxB). A targeted deletion mutant lacking this region was constructed by allelic exchange using the temperature-sensitive shuttle vector pSET4s. (B) S. oralis ATCC35037 wild-type (WT), spxB-deletion mutant (KO), or reverse mutant (Rev) was cultured in BHI broth or 5% RPMI1640 medium at 37°C for 18 h in a 5% CO 2 atmosphere. Concentrations of H 2 O 2 in culture supernatants were quantitatively determined using a hydrogen peroxide colorimetric detection kit. Data are shown as the mean ± SD of triplicate samples. *p

    Article Snippet: Cell culture The human monocyte cell line THP-1 cells were purchased from RIKEN Bioresource Center and cultured in RPMI1640 medium (Invitrogen, Carlsbad, CA, USA) supplemented with 5% fetal bovine serum (FBS) (Invitrogen) (5% FBS RPMI1640), penicillin (100 U/ml), and streptomycin (100 µg/ml) at 37°C in a 5% CO2 atmosphere.

    Techniques: Mutagenesis, Construct, Plasmid Preparation, Cell Culture

    Dose response curve of U937 cells and PBMC after treatment with F2. Log phase U937 cells and PBMC (2.5×10 4 /100 µl of RPMI 1640 medium/well) were treated with F2 fraction of n-butanol extract [(0–50 µg/ml; 24, 48, 72 h for U937), and (0–100 µg/ml; 48 h for PBMC)] and cell viability was measured by MTT assay as described in Materials and methods . Each data point represents the Mean ± SEM of at least three independent experiments in duplicate.

    Journal: PLoS ONE

    Article Title: Apoptotic and Autophagic Effects of Sesbania grandiflora Flowers in Human Leukemic Cells

    doi: 10.1371/journal.pone.0071672

    Figure Lengend Snippet: Dose response curve of U937 cells and PBMC after treatment with F2. Log phase U937 cells and PBMC (2.5×10 4 /100 µl of RPMI 1640 medium/well) were treated with F2 fraction of n-butanol extract [(0–50 µg/ml; 24, 48, 72 h for U937), and (0–100 µg/ml; 48 h for PBMC)] and cell viability was measured by MTT assay as described in Materials and methods . Each data point represents the Mean ± SEM of at least three independent experiments in duplicate.

    Article Snippet: Pen strep, RPMI 1640, DMEM, Heat inactivated Fetal Bovine Serum (FBS), 5, 5′, 6, 6′-tetrachloro-1,1′, 3, 3′-tetraethylbenzimidazolylcarbocyanine iodide (JC-1), and 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate (CM-H2 DCFDA) were obtained from Invitrogen (Carlsbad, CA, USA).

    Techniques: MTT Assay

    Involvement of caspase-dependent and caspase-independent modes of cell death. (A) Caspase activation. Activity of caspase-3, -8, -9 was measured in control and F2 (18.6 µg/ml) treated U937 cells (2.5×10 5 /ml) using colorimetric tetrapeptide substrates. Results are expressed as mean ± SEM from three independent experiments. (B) Cleavage of PARP by F2. Cell lysates were prepared and subjected to western blot analysis to check the cleavage of PARP. Data shown are from one of the three experiments. (C) Effect of pan caspase inhibitor on F2 induced cytotoxicity. Cells (2.5×10 4 /100 µl of RPMI 1640 medium/well) were pre-incubated with pan caspase inhibitor Z-VAD-FMK (20 µM; 4 h) followed by treatment with F2 (0–100 µg/ml; 48 h). Cell viability was evaluated by MTT assay. Histograms represent percentage cell viability (Mean ± SEM) obtained at the IC 50 concentration of F2 (18.6 µg/ml) and has been derived from at least three experiments in duplicate. (D) Effect of pan caspase inhibitor on F2 induced apoptosis. Cells were treated with F2 (18.6 µg/ml; 48 h) with or without pre-incubation using Z-VAD-FMK (20 µM; 4 h). They were co-stained with Annexin V-FITC and PI followed by analysis using flow cytometry. Histograms represent percentage of apoptotic cells and have been derived from at least three experiments (***p

    Journal: PLoS ONE

    Article Title: Apoptotic and Autophagic Effects of Sesbania grandiflora Flowers in Human Leukemic Cells

    doi: 10.1371/journal.pone.0071672

    Figure Lengend Snippet: Involvement of caspase-dependent and caspase-independent modes of cell death. (A) Caspase activation. Activity of caspase-3, -8, -9 was measured in control and F2 (18.6 µg/ml) treated U937 cells (2.5×10 5 /ml) using colorimetric tetrapeptide substrates. Results are expressed as mean ± SEM from three independent experiments. (B) Cleavage of PARP by F2. Cell lysates were prepared and subjected to western blot analysis to check the cleavage of PARP. Data shown are from one of the three experiments. (C) Effect of pan caspase inhibitor on F2 induced cytotoxicity. Cells (2.5×10 4 /100 µl of RPMI 1640 medium/well) were pre-incubated with pan caspase inhibitor Z-VAD-FMK (20 µM; 4 h) followed by treatment with F2 (0–100 µg/ml; 48 h). Cell viability was evaluated by MTT assay. Histograms represent percentage cell viability (Mean ± SEM) obtained at the IC 50 concentration of F2 (18.6 µg/ml) and has been derived from at least three experiments in duplicate. (D) Effect of pan caspase inhibitor on F2 induced apoptosis. Cells were treated with F2 (18.6 µg/ml; 48 h) with or without pre-incubation using Z-VAD-FMK (20 µM; 4 h). They were co-stained with Annexin V-FITC and PI followed by analysis using flow cytometry. Histograms represent percentage of apoptotic cells and have been derived from at least three experiments (***p

    Article Snippet: Pen strep, RPMI 1640, DMEM, Heat inactivated Fetal Bovine Serum (FBS), 5, 5′, 6, 6′-tetrachloro-1,1′, 3, 3′-tetraethylbenzimidazolylcarbocyanine iodide (JC-1), and 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate (CM-H2 DCFDA) were obtained from Invitrogen (Carlsbad, CA, USA).

    Techniques: Activation Assay, Activity Assay, Western Blot, Incubation, MTT Assay, Concentration Assay, Derivative Assay, Staining, Flow Cytometry, Cytometry

    Foam cell formation from macrophages by PLA2G3, PLA2G5 and PLA2G10. A , LDL (2 mg/ml) was incubated with or without recombinant human PLA2G3 (sPLA 2 -III) and PLA2G5 (sPLA 2 -V) for 24 h at 37 °C. Then the NEFA released was quantified ( n = 2). B and C , J774 cells were cultured for 24 h in the presence or absence of LDL (200 μg/ml) that had been incubated for 24 h with or without PLA2G3 or PLA2G5. Then the cells were homogenized, and cholesteryl ester contents were quantified ( n = 2) ( B ). Replicate cells were fixed and stained with oil red O ( C ). Ac-LDL (50 μg/ml) was used as a positive control for foam cell formation. Magnification was ×200. D , peritoneal macrophages obtained from PLA2G3 Tg ( III-Tg ) mice and PLA2G10 Tg ( X-Tg ) mice as well as WT mice were placed in RPMI1640 containing 10% lipoprotein-deficient fetal bovine serum and were incubated with or without 200 μg/ml LDL or 50 μg/ml Ac-LDL. After 24 h, the cells were stained with oil red O (magnification, ×200). Magnified views of individual macrophages are shown in the inset. E , PGE 2 production by macrophages from PLA2G3 Tg ( left ) and PLA2G10 Tg ( right ) mice in comparison with WT mice; macrophages were incubated for 24 h in the presence (+) or absence (-) of 200 μg/ml LDL (mean ± S.E., n = 3–5; * , p

    Journal: The Journal of Biological Chemistry

    Article Title: Analyses of Group III Secreted Phospholipase A2 Transgenic Mice Reveal Potential Participation of This Enzyme in Plasma Lipoprotein Modification, Macrophage Foam Cell Formation, and Atherosclerosis * Transgenic Mice Reveal Potential Participation of This Enzyme in Plasma Lipoprotein Modification, Macrophage Foam Cell Formation, and Atherosclerosis * S⃞

    doi: 10.1074/jbc.M804628200

    Figure Lengend Snippet: Foam cell formation from macrophages by PLA2G3, PLA2G5 and PLA2G10. A , LDL (2 mg/ml) was incubated with or without recombinant human PLA2G3 (sPLA 2 -III) and PLA2G5 (sPLA 2 -V) for 24 h at 37 °C. Then the NEFA released was quantified ( n = 2). B and C , J774 cells were cultured for 24 h in the presence or absence of LDL (200 μg/ml) that had been incubated for 24 h with or without PLA2G3 or PLA2G5. Then the cells were homogenized, and cholesteryl ester contents were quantified ( n = 2) ( B ). Replicate cells were fixed and stained with oil red O ( C ). Ac-LDL (50 μg/ml) was used as a positive control for foam cell formation. Magnification was ×200. D , peritoneal macrophages obtained from PLA2G3 Tg ( III-Tg ) mice and PLA2G10 Tg ( X-Tg ) mice as well as WT mice were placed in RPMI1640 containing 10% lipoprotein-deficient fetal bovine serum and were incubated with or without 200 μg/ml LDL or 50 μg/ml Ac-LDL. After 24 h, the cells were stained with oil red O (magnification, ×200). Magnified views of individual macrophages are shown in the inset. E , PGE 2 production by macrophages from PLA2G3 Tg ( left ) and PLA2G10 Tg ( right ) mice in comparison with WT mice; macrophages were incubated for 24 h in the presence (+) or absence (-) of 200 μg/ml LDL (mean ± S.E., n = 3–5; * , p

    Article Snippet: Peritoneal cells isolated from mice were seeded in 2 × 106 cells/well in 12-well plates (Iwaki) for 2 h in RPMI1640 containing 10% lipoprotein-deficient fetal bovine serum (Sigma), and adherent cells were used as primary macrophages.

    Techniques: Incubation, Recombinant, Cell Culture, Staining, Positive Control, Mouse Assay