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  • 95
    Lonza rpmi 1640
    Rpmi 1640, supplied by Lonza, used in various techniques. Bioz Stars score: 95/100, based on 5862 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi 1640/product/Lonza
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    99
    ATCC rpmi 1640 medium
    Rpmi 1640 Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2163 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi 1640 medium/product/ATCC
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    rpmi 1640 medium - by Bioz Stars, 2021-02
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    99
    Thermo Fisher rpmi 1640 medium
    Rpmi 1640 Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 100450 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi 1640 medium/product/Thermo Fisher
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    99
    Thermo Fisher rpmi 1640
    Rpmi 1640, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 91082 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi 1640/product/Thermo Fisher
    Average 99 stars, based on 91082 article reviews
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    rpmi 1640 - by Bioz Stars, 2021-02
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    99
    Millipore rpmi 1640 medium
    Rpmi 1640 Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 30869 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi 1640 medium/product/Millipore
    Average 99 stars, based on 30869 article reviews
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    rpmi 1640 medium - by Bioz Stars, 2021-02
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    99
    Thermo Fisher rpmi
    Rpmi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 29265 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi/product/Thermo Fisher
    Average 99 stars, based on 29265 article reviews
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    rpmi - by Bioz Stars, 2021-02
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    99
    GE Healthcare rpmi 1640 medium
    Rpmi 1640 Medium, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 12494 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi 1640 medium/product/GE Healthcare
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    rpmi 1640 medium - by Bioz Stars, 2021-02
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    97
    GE Healthcare rpmi 1640
    Rpmi 1640, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 97/100, based on 9823 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi 1640/product/GE Healthcare
    Average 97 stars, based on 9823 article reviews
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    99
    Thermo Fisher rpmi1640
    Rpmi1640, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi1640/product/Thermo Fisher
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    94
    Lonza rpmi 1640 medium
    Rpmi 1640 Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 94/100, based on 5133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi 1640 medium/product/Lonza
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    rpmi 1640 medium - by Bioz Stars, 2021-02
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    92
    Mediatech rpmi 1640
    Effect of various QNC concentrations on  C. albicans  SC5314 filamentation on solid medium and 1,024 μg/ml QNC in liquid medium. (A) Growth of SC5314 colonies on YPD plus 10% fetal calf serum (FCS) solid medium, M199, and RPMI 1640, all with various
    Rpmi 1640, supplied by Mediatech, used in various techniques. Bioz Stars score: 92/100, based on 3945 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC rpmi 1640
    Effect of various QNC concentrations on  C. albicans  SC5314 filamentation on solid medium and 1,024 μg/ml QNC in liquid medium. (A) Growth of SC5314 colonies on YPD plus 10% fetal calf serum (FCS) solid medium, M199, and RPMI 1640, all with various
    Rpmi 1640, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1528 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore rpmi
    Effect of various QNC concentrations on  C. albicans  SC5314 filamentation on solid medium and 1,024 μg/ml QNC in liquid medium. (A) Growth of SC5314 colonies on YPD plus 10% fetal calf serum (FCS) solid medium, M199, and RPMI 1640, all with various
    Rpmi, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 12725 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi/product/Millipore
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    92
    Corning Life Sciences rpmi 1640
    Inhibition of IL-1β activity reverses macrophage-induced cytokine release by human adipocytes. Differentiated adipocytes were incubated with RPMI 1640 (control), MC medium, MC medium neutralized by IL-1β antibody (2 μg/ml), mouse IgG (2 μg/ml, as negative control), or MC medium alone (without cells) for 24 h. The release of IL-6 ( A ), MCP-1 ( B ), IL-8 ( C ) and RANTES ( D ) by adipocytes was measured as protein concentrations in cell culture medium by ELISAs. Data are means ± SD ( n  = 6 per group). *** P
    Rpmi 1640, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 2500 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi 1640/product/Corning Life Sciences
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    92
    Cellgro rpmi 1640
    Staining the cell wall of the tetR-SSO2 hyphae The tetR-SSO2 strain was grown in buffered <t>RPMI-1640,</t> in the absence (−DOX) and presence (+DOX) of doxycycline for 5 hours. Following incubation, the cells were stained with calcofluor white (0.5 μg μl −1 ) and subsequently imaged under fluorescence with a DAPI filter. Calcofluor white predominantly stains chitin in the fungal cell wall, a component that confers rigidity to the cell. The white arrow highlights the absence of chitin from the growing hyphal tip of the tetR-SSO2 strain grown under de-repressing conditions.
    Rpmi 1640, supplied by Cellgro, used in various techniques. Bioz Stars score: 92/100, based on 2551 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi 1640/product/Cellgro
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    92
    Mediatech rpmi 1640 medium
    Figure 6.  Cell cycle kinetics of cancer cells during seeding on Gelffoam®. FUCCI-expressing cancer cells (5 × 10 6 ) were placed on Gelfoam® (1 × 1 cm) in RPMI 1640 medium. ( A ) Low-magnification image of an overview
    Rpmi 1640 Medium, supplied by Mediatech, used in various techniques. Bioz Stars score: 92/100, based on 2810 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi 1640 medium/product/Mediatech
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    rpmi 1640 medium - by Bioz Stars, 2021-02
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    N/A
    established from the peripheral blood of a 16 year old woman with acute lymphoblastic leukemia ALL in 1972 Exome and RNA sequence data are available see Ref 18187 and Exome
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    N/A
    RPMI 8226 cell was lysed in RIPA buffer and mixed with equivalent volumes of 2X SDS PAGE loading buffer prior to lyophilization
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    N/A
    established from an involved lymph node of an 18 year old Caucasian woman with malignant melanoma in 1971
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    N/A
    established from the peripheral blood of a 61 year old man with multiple myeloma IgG lambda type at diagnosis in 1966 described in the literature to produce and secrete only
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    Image Search Results


    Effect of various QNC concentrations on  C. albicans  SC5314 filamentation on solid medium and 1,024 μg/ml QNC in liquid medium. (A) Growth of SC5314 colonies on YPD plus 10% fetal calf serum (FCS) solid medium, M199, and RPMI 1640, all with various

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Quinacrine Inhibits Candida albicans Growth and Filamentation at Neutral pH

    doi: 10.1128/AAC.03083-14

    Figure Lengend Snippet: Effect of various QNC concentrations on C. albicans SC5314 filamentation on solid medium and 1,024 μg/ml QNC in liquid medium. (A) Growth of SC5314 colonies on YPD plus 10% fetal calf serum (FCS) solid medium, M199, and RPMI 1640, all with various

    Article Snippet: For the biofilm studies, a standardized cell suspension was made in RPMI 1640 (Mediatech) supplemented with l -glutamine (Gibco) and buffered with 165 mM morpholinepropanesulfonic acid (MOPS) (Sigma-Aldrich) to pH 7.0, to attain a cell density of 1 × 106 cells/ml.

    Techniques:

    Effect of 1,024 μg/ml QNC on  C. albicans  SC5314 biofilm formation in pH-buffered RPMI 1640. The effects of various concentrations of QNC against the formation of  C. albicans  SC5314 biofilms were assessed in a 96-well static microplate model. The

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Quinacrine Inhibits Candida albicans Growth and Filamentation at Neutral pH

    doi: 10.1128/AAC.03083-14

    Figure Lengend Snippet: Effect of 1,024 μg/ml QNC on C. albicans SC5314 biofilm formation in pH-buffered RPMI 1640. The effects of various concentrations of QNC against the formation of C. albicans SC5314 biofilms were assessed in a 96-well static microplate model. The

    Article Snippet: For the biofilm studies, a standardized cell suspension was made in RPMI 1640 (Mediatech) supplemented with l -glutamine (Gibco) and buffered with 165 mM morpholinepropanesulfonic acid (MOPS) (Sigma-Aldrich) to pH 7.0, to attain a cell density of 1 × 106 cells/ml.

    Techniques:

    Inhibition of IL-1β activity reverses macrophage-induced cytokine release by human adipocytes. Differentiated adipocytes were incubated with RPMI 1640 (control), MC medium, MC medium neutralized by IL-1β antibody (2 μg/ml), mouse IgG (2 μg/ml, as negative control), or MC medium alone (without cells) for 24 h. The release of IL-6 ( A ), MCP-1 ( B ), IL-8 ( C ) and RANTES ( D ) by adipocytes was measured as protein concentrations in cell culture medium by ELISAs. Data are means ± SD ( n  = 6 per group). *** P

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Interleukin-1β mediates macrophage-induced impairment of insulin signaling in human primary adipocytes

    doi: 10.1152/ajpendo.00430.2013

    Figure Lengend Snippet: Inhibition of IL-1β activity reverses macrophage-induced cytokine release by human adipocytes. Differentiated adipocytes were incubated with RPMI 1640 (control), MC medium, MC medium neutralized by IL-1β antibody (2 μg/ml), mouse IgG (2 μg/ml, as negative control), or MC medium alone (without cells) for 24 h. The release of IL-6 ( A ), MCP-1 ( B ), IL-8 ( C ) and RANTES ( D ) by adipocytes was measured as protein concentrations in cell culture medium by ELISAs. Data are means ± SD ( n = 6 per group). *** P

    Article Snippet: The PBMCs (peripheral blood mononuclear cells) were isolated from the buffy layer and washed once with RPMI-1640 (without FBS or l -glutamine) by centrifuging at 350 g for 10 min. Monocytes were allowed to adhere to 25-cm2 tissue culture flasks (Corning, Amsterdam, The Netherlands) for 3 days, and then nonadherent cells were removed by several washes with primary macrophage medium (RPMI-1640 without phenol red, supplemented with 10% FCS, 2 mM l -glutamine, and 20 mM HEPES).

    Techniques: Inhibition, Activity Assay, Incubation, Negative Control, Cell Culture

    Inhibition of IL-1β activity abolishes the inhibitory effect of macrophages on protein expression of IRS-1, PI3K p85α, and GLUT4 by human adipocytes. Differentiated adipocytes were incubated with RPMI 1640 medium (control), MC medium, MC medium neutralized by IL-1β antibody (2 μg/ml), or mouse IgG (2 μg/ml, as negative control) for 24 h. Cell lysates were analyzed by Western blotting and densitometry using antibodies to IRS-1 ( A, B ), PI3K p85α ( C, D ), and GLUT4 ( E, F ). Separate groups of adipocytes were treated with various agents as above, followed by stimulation with or without insulin (167 nmol/l) for 5 min; Akt phosphorylation at Ser 473  (pAkt) was analyzed by Western blotting and densitometry. Total Akt and GAPDH were used as loading controls ( G, H ). Representative blots are shown; data are means ± SE ( n  = 3per group). * P

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Interleukin-1β mediates macrophage-induced impairment of insulin signaling in human primary adipocytes

    doi: 10.1152/ajpendo.00430.2013

    Figure Lengend Snippet: Inhibition of IL-1β activity abolishes the inhibitory effect of macrophages on protein expression of IRS-1, PI3K p85α, and GLUT4 by human adipocytes. Differentiated adipocytes were incubated with RPMI 1640 medium (control), MC medium, MC medium neutralized by IL-1β antibody (2 μg/ml), or mouse IgG (2 μg/ml, as negative control) for 24 h. Cell lysates were analyzed by Western blotting and densitometry using antibodies to IRS-1 ( A, B ), PI3K p85α ( C, D ), and GLUT4 ( E, F ). Separate groups of adipocytes were treated with various agents as above, followed by stimulation with or without insulin (167 nmol/l) for 5 min; Akt phosphorylation at Ser 473 (pAkt) was analyzed by Western blotting and densitometry. Total Akt and GAPDH were used as loading controls ( G, H ). Representative blots are shown; data are means ± SE ( n = 3per group). * P

    Article Snippet: The PBMCs (peripheral blood mononuclear cells) were isolated from the buffy layer and washed once with RPMI-1640 (without FBS or l -glutamine) by centrifuging at 350 g for 10 min. Monocytes were allowed to adhere to 25-cm2 tissue culture flasks (Corning, Amsterdam, The Netherlands) for 3 days, and then nonadherent cells were removed by several washes with primary macrophage medium (RPMI-1640 without phenol red, supplemented with 10% FCS, 2 mM l -glutamine, and 20 mM HEPES).

    Techniques: Inhibition, Activity Assay, Expressing, Incubation, Negative Control, Western Blot

    Inhibition of IL-1β production by THP-1 macrophages reduces the effect of MC medium on protein expression of IRS-1, PI3K p85α, and GLUT4 and cytokine release by adipocytes. THP-1 macrophages were incubated with RPMI 1640 (control) or caspase-1 inhibitor (50 μM) for 48 h (with freshly changed medium at 24 h), and the culture medium was collected. Differentiated adipocytes were then treated with RPMI 1640 (control), MC medium, or MC medium in the presence of caspase-1 inhibitor for 24 h. Cell lysates were analyzed by Western blotting and densitometry, using antibodies to IRS-1, PI3K p85α, and GLUT4  (A–D ). Data are expressed as means ± SE ( n  = 6 per group). Protein release of IL-6 ( E ), IL-8 ( F ), and RANTES ( G ) by adipocytes was measured by ELISAs. Data are expressed as means ± SD ( n  = 6 per group). * P

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Interleukin-1β mediates macrophage-induced impairment of insulin signaling in human primary adipocytes

    doi: 10.1152/ajpendo.00430.2013

    Figure Lengend Snippet: Inhibition of IL-1β production by THP-1 macrophages reduces the effect of MC medium on protein expression of IRS-1, PI3K p85α, and GLUT4 and cytokine release by adipocytes. THP-1 macrophages were incubated with RPMI 1640 (control) or caspase-1 inhibitor (50 μM) for 48 h (with freshly changed medium at 24 h), and the culture medium was collected. Differentiated adipocytes were then treated with RPMI 1640 (control), MC medium, or MC medium in the presence of caspase-1 inhibitor for 24 h. Cell lysates were analyzed by Western blotting and densitometry, using antibodies to IRS-1, PI3K p85α, and GLUT4 (A–D ). Data are expressed as means ± SE ( n = 6 per group). Protein release of IL-6 ( E ), IL-8 ( F ), and RANTES ( G ) by adipocytes was measured by ELISAs. Data are expressed as means ± SD ( n = 6 per group). * P

    Article Snippet: The PBMCs (peripheral blood mononuclear cells) were isolated from the buffy layer and washed once with RPMI-1640 (without FBS or l -glutamine) by centrifuging at 350 g for 10 min. Monocytes were allowed to adhere to 25-cm2 tissue culture flasks (Corning, Amsterdam, The Netherlands) for 3 days, and then nonadherent cells were removed by several washes with primary macrophage medium (RPMI-1640 without phenol red, supplemented with 10% FCS, 2 mM l -glutamine, and 20 mM HEPES).

    Techniques: Inhibition, Expressing, Incubation, Western Blot

    MC medium generated from human PBMC-derived macrophages decreases protein expression of insulin signaling molecules and induces cytokine release by human adipocytes and the effect of blocking IL-1β. Differentiated human adipocytes were treated with RPMI 1640 (control), MC medium, MC medium neutralized by IL-1β antibody (15 μg/ml), MC medium neutralized by IL-1β antibody (7.5 μg/ml), and TNFα (7.5 μg/ml), mouse IgG (15 μg/ml), or IL-1RA (1 μg/ml) for 24 h. Cell lysates were analyzed by Western blotting and densitometry, using antibodies to IRS-1 ( A, B ) and GLUT4 ( C ,  D ). For measuring basal and insulin-stimulated Akt phosphorylation, adipocytes were incubated with RPMI 1640 or MC medium for 24 h before being stimulated with insulin (167 nmol/l) for 5 min; Akt phosphorylation at Ser 473  (pAkt) was analyzed by Western blotting and densitometry; GAPDH was used as loading controls ( E, F ). Representative blots are shown. Data are means ± SE ( n  = 3 per group). * P

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Interleukin-1β mediates macrophage-induced impairment of insulin signaling in human primary adipocytes

    doi: 10.1152/ajpendo.00430.2013

    Figure Lengend Snippet: MC medium generated from human PBMC-derived macrophages decreases protein expression of insulin signaling molecules and induces cytokine release by human adipocytes and the effect of blocking IL-1β. Differentiated human adipocytes were treated with RPMI 1640 (control), MC medium, MC medium neutralized by IL-1β antibody (15 μg/ml), MC medium neutralized by IL-1β antibody (7.5 μg/ml), and TNFα (7.5 μg/ml), mouse IgG (15 μg/ml), or IL-1RA (1 μg/ml) for 24 h. Cell lysates were analyzed by Western blotting and densitometry, using antibodies to IRS-1 ( A, B ) and GLUT4 ( C , D ). For measuring basal and insulin-stimulated Akt phosphorylation, adipocytes were incubated with RPMI 1640 or MC medium for 24 h before being stimulated with insulin (167 nmol/l) for 5 min; Akt phosphorylation at Ser 473 (pAkt) was analyzed by Western blotting and densitometry; GAPDH was used as loading controls ( E, F ). Representative blots are shown. Data are means ± SE ( n = 3 per group). * P

    Article Snippet: The PBMCs (peripheral blood mononuclear cells) were isolated from the buffy layer and washed once with RPMI-1640 (without FBS or l -glutamine) by centrifuging at 350 g for 10 min. Monocytes were allowed to adhere to 25-cm2 tissue culture flasks (Corning, Amsterdam, The Netherlands) for 3 days, and then nonadherent cells were removed by several washes with primary macrophage medium (RPMI-1640 without phenol red, supplemented with 10% FCS, 2 mM l -glutamine, and 20 mM HEPES).

    Techniques: Generated, Derivative Assay, Expressing, Blocking Assay, Western Blot, Incubation

    IL-1β mediates macrophage-induced alteration of glucose and lipid metabolism in human adipocytes. Adipocytes were treated with RPMI 1640 (control), THP-1 MC medium, MC medium neutralized by IL-1β antibody (2 μg/ml), MC medium with IgG (2 μg/ml), RPMI 1640 only (control), or RPMI 1640 with IL-1β (2 ng/ml) for 24 h. Glucose consumption was measured as the glucose concentration in culture medium by glucose oxidase method ( A ). Lipolysis was determined as glycerol release into culture medium ( B, C ). Data are means ± SE ( n  = 6 per group). ** P

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Interleukin-1β mediates macrophage-induced impairment of insulin signaling in human primary adipocytes

    doi: 10.1152/ajpendo.00430.2013

    Figure Lengend Snippet: IL-1β mediates macrophage-induced alteration of glucose and lipid metabolism in human adipocytes. Adipocytes were treated with RPMI 1640 (control), THP-1 MC medium, MC medium neutralized by IL-1β antibody (2 μg/ml), MC medium with IgG (2 μg/ml), RPMI 1640 only (control), or RPMI 1640 with IL-1β (2 ng/ml) for 24 h. Glucose consumption was measured as the glucose concentration in culture medium by glucose oxidase method ( A ). Lipolysis was determined as glycerol release into culture medium ( B, C ). Data are means ± SE ( n = 6 per group). ** P

    Article Snippet: The PBMCs (peripheral blood mononuclear cells) were isolated from the buffy layer and washed once with RPMI-1640 (without FBS or l -glutamine) by centrifuging at 350 g for 10 min. Monocytes were allowed to adhere to 25-cm2 tissue culture flasks (Corning, Amsterdam, The Netherlands) for 3 days, and then nonadherent cells were removed by several washes with primary macrophage medium (RPMI-1640 without phenol red, supplemented with 10% FCS, 2 mM l -glutamine, and 20 mM HEPES).

    Techniques: Concentration Assay

    Macrophage-conditioned (MC) medium reduces protein expression of insulin signaling molecules in human adipocytes. Adipocytes (at  day 12  postdifferentiation) were treated with RPMI 1640 medium (control) or THP-1 MC medium (25%) for 24 h. Cell lysates were analyzed by Western blotting and densitometry, using antibodies to IRS-1 and PI3K p85α ( A, B, C ) and GLUT4 ( D, E ). For measuring basal and insulin-stimulated Akt phosphorylation, adipocytes were incubated with RPMI 1640 or MC medium for 24 h before being stimulated with insulin (167 nmol/l) for 5 min; Akt phosphorylation at Ser 473  (pAkt) was analyzed by Western blotting and densitometry. Total Akt and GAPDH were used as loading controls ( F, G ). Representative blots are shown; data are means ± SE ( n  = 3 per group). * P

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Interleukin-1β mediates macrophage-induced impairment of insulin signaling in human primary adipocytes

    doi: 10.1152/ajpendo.00430.2013

    Figure Lengend Snippet: Macrophage-conditioned (MC) medium reduces protein expression of insulin signaling molecules in human adipocytes. Adipocytes (at day 12 postdifferentiation) were treated with RPMI 1640 medium (control) or THP-1 MC medium (25%) for 24 h. Cell lysates were analyzed by Western blotting and densitometry, using antibodies to IRS-1 and PI3K p85α ( A, B, C ) and GLUT4 ( D, E ). For measuring basal and insulin-stimulated Akt phosphorylation, adipocytes were incubated with RPMI 1640 or MC medium for 24 h before being stimulated with insulin (167 nmol/l) for 5 min; Akt phosphorylation at Ser 473 (pAkt) was analyzed by Western blotting and densitometry. Total Akt and GAPDH were used as loading controls ( F, G ). Representative blots are shown; data are means ± SE ( n = 3 per group). * P

    Article Snippet: The PBMCs (peripheral blood mononuclear cells) were isolated from the buffy layer and washed once with RPMI-1640 (without FBS or l -glutamine) by centrifuging at 350 g for 10 min. Monocytes were allowed to adhere to 25-cm2 tissue culture flasks (Corning, Amsterdam, The Netherlands) for 3 days, and then nonadherent cells were removed by several washes with primary macrophage medium (RPMI-1640 without phenol red, supplemented with 10% FCS, 2 mM l -glutamine, and 20 mM HEPES).

    Techniques: Expressing, Western Blot, Incubation

    Inhibition of IL-1β activity reduces the effect of macrophages on expression profile of genes related to glucose metabolism, insulin signaling, and inflammation in human adipocytes. To block the activity of IL-1β, MC medium was preincubated with an IL-1β neutralizing antibody (2 μg/ml) for 1 h at 37°C. Differentiated adipocytes were then treated with RPMI 1640 (control), MC medium, or MC medium neutralized by IL-1β antibody for 24 h. Expression levels of genes involved in glucose metabolism, insulin signaling, lipid metabolism, insulin sensitivity ( A ), and inflammation ( B ) were determined using a PCR array. Data are expressed as fold changes ( n  = 3 per group). * P

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Interleukin-1β mediates macrophage-induced impairment of insulin signaling in human primary adipocytes

    doi: 10.1152/ajpendo.00430.2013

    Figure Lengend Snippet: Inhibition of IL-1β activity reduces the effect of macrophages on expression profile of genes related to glucose metabolism, insulin signaling, and inflammation in human adipocytes. To block the activity of IL-1β, MC medium was preincubated with an IL-1β neutralizing antibody (2 μg/ml) for 1 h at 37°C. Differentiated adipocytes were then treated with RPMI 1640 (control), MC medium, or MC medium neutralized by IL-1β antibody for 24 h. Expression levels of genes involved in glucose metabolism, insulin signaling, lipid metabolism, insulin sensitivity ( A ), and inflammation ( B ) were determined using a PCR array. Data are expressed as fold changes ( n = 3 per group). * P

    Article Snippet: The PBMCs (peripheral blood mononuclear cells) were isolated from the buffy layer and washed once with RPMI-1640 (without FBS or l -glutamine) by centrifuging at 350 g for 10 min. Monocytes were allowed to adhere to 25-cm2 tissue culture flasks (Corning, Amsterdam, The Netherlands) for 3 days, and then nonadherent cells were removed by several washes with primary macrophage medium (RPMI-1640 without phenol red, supplemented with 10% FCS, 2 mM l -glutamine, and 20 mM HEPES).

    Techniques: Inhibition, Activity Assay, Expressing, Blocking Assay, Polymerase Chain Reaction

    Blocking IL-1 receptor with IL-1 receptor antagonist reverses the effects of MC medium on protein expression of IRS-1, PI3K p85α, and GLUT4 and cytokine release by human adipocytes. Differentiated adipocytes were incubated with RPMI 1640 (control), MC medium, or MC medium with a recombinant IL-1 receptor antagonist (IL-1RA; 1 μg/ml) for 24 h. Cell lysates were analyzed by Western blotting and densitometry, using antibodies to IRS-1 ( A, B ), PI3K p85α ( C ,  D ), and GLUT4 ( E, F ). On the blot shown, there is an empty lane between MC and MC+IL-1RA groups ( C ). Data are expressed as means ± SE ( n  = 3 per group). * P

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Interleukin-1β mediates macrophage-induced impairment of insulin signaling in human primary adipocytes

    doi: 10.1152/ajpendo.00430.2013

    Figure Lengend Snippet: Blocking IL-1 receptor with IL-1 receptor antagonist reverses the effects of MC medium on protein expression of IRS-1, PI3K p85α, and GLUT4 and cytokine release by human adipocytes. Differentiated adipocytes were incubated with RPMI 1640 (control), MC medium, or MC medium with a recombinant IL-1 receptor antagonist (IL-1RA; 1 μg/ml) for 24 h. Cell lysates were analyzed by Western blotting and densitometry, using antibodies to IRS-1 ( A, B ), PI3K p85α ( C , D ), and GLUT4 ( E, F ). On the blot shown, there is an empty lane between MC and MC+IL-1RA groups ( C ). Data are expressed as means ± SE ( n = 3 per group). * P

    Article Snippet: The PBMCs (peripheral blood mononuclear cells) were isolated from the buffy layer and washed once with RPMI-1640 (without FBS or l -glutamine) by centrifuging at 350 g for 10 min. Monocytes were allowed to adhere to 25-cm2 tissue culture flasks (Corning, Amsterdam, The Netherlands) for 3 days, and then nonadherent cells were removed by several washes with primary macrophage medium (RPMI-1640 without phenol red, supplemented with 10% FCS, 2 mM l -glutamine, and 20 mM HEPES).

    Techniques: Blocking Assay, Expressing, Incubation, Recombinant, Western Blot

    Staining the cell wall of the tetR-SSO2 hyphae The tetR-SSO2 strain was grown in buffered RPMI-1640, in the absence (−DOX) and presence (+DOX) of doxycycline for 5 hours. Following incubation, the cells were stained with calcofluor white (0.5 μg μl −1 ) and subsequently imaged under fluorescence with a DAPI filter. Calcofluor white predominantly stains chitin in the fungal cell wall, a component that confers rigidity to the cell. The white arrow highlights the absence of chitin from the growing hyphal tip of the tetR-SSO2 strain grown under de-repressing conditions.

    Journal: FEMS yeast research

    Article Title: Secretion and filamentation are mediated by the Candida albicans t-SNAREs Sso2p and Sec9p

    doi: 10.1111/1567-1364.12165

    Figure Lengend Snippet: Staining the cell wall of the tetR-SSO2 hyphae The tetR-SSO2 strain was grown in buffered RPMI-1640, in the absence (−DOX) and presence (+DOX) of doxycycline for 5 hours. Following incubation, the cells were stained with calcofluor white (0.5 μg μl −1 ) and subsequently imaged under fluorescence with a DAPI filter. Calcofluor white predominantly stains chitin in the fungal cell wall, a component that confers rigidity to the cell. The white arrow highlights the absence of chitin from the growing hyphal tip of the tetR-SSO2 strain grown under de-repressing conditions.

    Article Snippet: Filamentation was assayed at 37 °C in liquid RPMI-1640 supplemented with L-glutamine (Cellgro, Manassas, VA) and buffered with 165 mM MOPS (Sigma, St. Louis, MO) to pH 7.0 (referred to as “buffered RPMI-1640” throughout the manuscript).

    Techniques: Staining, Incubation, Fluorescence

    Filamentation of tetR-SSO2 and tetR-SEC9 in liquid medium Cells at a concentration of 5×10 6 cells ml −1 were grown in buffered RPMI-1640 at 37 °C with shaking at 200rpm. Filamentation was followed at hourly intervals by DIC microscopy. Representative DIC images using a 63X oil objective, taken at T=5 hr, are shown. Formation of the bulbous tip in the tetR-SSO2 strain grown in the presence of DOX is typical (arrow). Scale bar shown represents 5 μm.

    Journal: FEMS yeast research

    Article Title: Secretion and filamentation are mediated by the Candida albicans t-SNAREs Sso2p and Sec9p

    doi: 10.1111/1567-1364.12165

    Figure Lengend Snippet: Filamentation of tetR-SSO2 and tetR-SEC9 in liquid medium Cells at a concentration of 5×10 6 cells ml −1 were grown in buffered RPMI-1640 at 37 °C with shaking at 200rpm. Filamentation was followed at hourly intervals by DIC microscopy. Representative DIC images using a 63X oil objective, taken at T=5 hr, are shown. Formation of the bulbous tip in the tetR-SSO2 strain grown in the presence of DOX is typical (arrow). Scale bar shown represents 5 μm.

    Article Snippet: Filamentation was assayed at 37 °C in liquid RPMI-1640 supplemented with L-glutamine (Cellgro, Manassas, VA) and buffered with 165 mM MOPS (Sigma, St. Louis, MO) to pH 7.0 (referred to as “buffered RPMI-1640” throughout the manuscript).

    Techniques: Concentration Assay, Microscopy

    Effect of loss-of-function of SSO2 or SEC9 on the C. albicans Spitzenkörper C. albicans MLC1 -GFP was used to visualize the C. albicans Spitzenkörper in background strains THE1-CIp10, tetR-SSO2, and tetR-SEC9. Filamentation was induced in buffered RPMI-1640, in the presence (+) or absence (−) of DOX, and the fate of the Spitzenkörper was assessed over 5 hours of incubation. Fluorescent images at the tip of the growing hyphae at the 5-hr timepoint are shown, with the corresponding DIC image. C. albicans Mlc1p-GFP localized to septin rings in the tetR-SSO2 cell grown under repressing conditions (arrow heads); the same structure stains with calcofluor white (data not shown).

    Journal: FEMS yeast research

    Article Title: Secretion and filamentation are mediated by the Candida albicans t-SNAREs Sso2p and Sec9p

    doi: 10.1111/1567-1364.12165

    Figure Lengend Snippet: Effect of loss-of-function of SSO2 or SEC9 on the C. albicans Spitzenkörper C. albicans MLC1 -GFP was used to visualize the C. albicans Spitzenkörper in background strains THE1-CIp10, tetR-SSO2, and tetR-SEC9. Filamentation was induced in buffered RPMI-1640, in the presence (+) or absence (−) of DOX, and the fate of the Spitzenkörper was assessed over 5 hours of incubation. Fluorescent images at the tip of the growing hyphae at the 5-hr timepoint are shown, with the corresponding DIC image. C. albicans Mlc1p-GFP localized to septin rings in the tetR-SSO2 cell grown under repressing conditions (arrow heads); the same structure stains with calcofluor white (data not shown).

    Article Snippet: Filamentation was assayed at 37 °C in liquid RPMI-1640 supplemented with L-glutamine (Cellgro, Manassas, VA) and buffered with 165 mM MOPS (Sigma, St. Louis, MO) to pH 7.0 (referred to as “buffered RPMI-1640” throughout the manuscript).

    Techniques: Incubation

    Cell morphology and cytokinesis defect in the absence of SSO2 and SEC9 Overnight cultures of strains grown in YPD were shifted to fresh media, with or without DOX. Cells were fixed and processed for ultrastructural analysis using thin-section electron microscopy. Note that images for hyphae are representative cross-sections; full length hyphae were not visualized in these TEMs due to curvature of the hyphae in and out of the z-axis. (A) Following incubation in YPD, tetR-SSO2 and tetR-SEC9 cells grown in the presence of DOX accumulate secretory vesicles (arrows) and produce wide-necked buds during cell division. Partial inward growth at the bud neck is also evident in tetR-SSO2 in the presence of DOX (double arrows). Bars represent a length of 500nm. tetR-SSO2 micrographs were imaged under a magnification of 20kX; tetR-SEC9 micrographs were imaged under a magnification of 30kX. (B) Following incubation in buffered RPMI-1640 to induce filamentation, tetR-SSO2 hyphal cells also accumulated secretory vesicles when grown in the presence of DOX (arrows), in contrast to tetR-SEC9 cells which appear like the wild-type controls (de-repressed state). Bars indicate 2 μm. All micrographs were imaged under a magnification of 12kX, except the tetR-SEC9 with DOX micrograph which was imaged under 6kX magnification.

    Journal: FEMS yeast research

    Article Title: Secretion and filamentation are mediated by the Candida albicans t-SNAREs Sso2p and Sec9p

    doi: 10.1111/1567-1364.12165

    Figure Lengend Snippet: Cell morphology and cytokinesis defect in the absence of SSO2 and SEC9 Overnight cultures of strains grown in YPD were shifted to fresh media, with or without DOX. Cells were fixed and processed for ultrastructural analysis using thin-section electron microscopy. Note that images for hyphae are representative cross-sections; full length hyphae were not visualized in these TEMs due to curvature of the hyphae in and out of the z-axis. (A) Following incubation in YPD, tetR-SSO2 and tetR-SEC9 cells grown in the presence of DOX accumulate secretory vesicles (arrows) and produce wide-necked buds during cell division. Partial inward growth at the bud neck is also evident in tetR-SSO2 in the presence of DOX (double arrows). Bars represent a length of 500nm. tetR-SSO2 micrographs were imaged under a magnification of 20kX; tetR-SEC9 micrographs were imaged under a magnification of 30kX. (B) Following incubation in buffered RPMI-1640 to induce filamentation, tetR-SSO2 hyphal cells also accumulated secretory vesicles when grown in the presence of DOX (arrows), in contrast to tetR-SEC9 cells which appear like the wild-type controls (de-repressed state). Bars indicate 2 μm. All micrographs were imaged under a magnification of 12kX, except the tetR-SEC9 with DOX micrograph which was imaged under 6kX magnification.

    Article Snippet: Filamentation was assayed at 37 °C in liquid RPMI-1640 supplemented with L-glutamine (Cellgro, Manassas, VA) and buffered with 165 mM MOPS (Sigma, St. Louis, MO) to pH 7.0 (referred to as “buffered RPMI-1640” throughout the manuscript).

    Techniques: Electron Microscopy, Incubation

    Figure 6.  Cell cycle kinetics of cancer cells during seeding on Gelffoam®. FUCCI-expressing cancer cells (5 × 10 6 ) were placed on Gelfoam® (1 × 1 cm) in RPMI 1640 medium. ( A ) Low-magnification image of an overview

    Journal: Cell Cycle

    Article Title: Invading cancer cells are predominantly in G0/G1 resulting in chemoresistance demonstrated by real-time FUCCI imaging

    doi: 10.4161/cc.27818

    Figure Lengend Snippet: Figure 6. Cell cycle kinetics of cancer cells during seeding on Gelffoam®. FUCCI-expressing cancer cells (5 × 10 6 ) were placed on Gelfoam® (1 × 1 cm) in RPMI 1640 medium. ( A ) Low-magnification image of an overview

    Article Snippet: FUCCI-expressing MKN45 cells were seeded on plastic plates for 2-dimensional culture in RPMI-1640 medium (Mediatech).

    Techniques: Expressing

    Figure 5.  Comparison of migration velocity of cancer cells in G 0 /G 1  phase and S/G 2 /M phases during cell division. FUCCI-expressing cancer cells (5 × 10 6 ) were placed on Gelfoam® (1 × 1 cm) in RPMI 1640 medium. ( A ) Cancer

    Journal: Cell Cycle

    Article Title: Invading cancer cells are predominantly in G0/G1 resulting in chemoresistance demonstrated by real-time FUCCI imaging

    doi: 10.4161/cc.27818

    Figure Lengend Snippet: Figure 5. Comparison of migration velocity of cancer cells in G 0 /G 1 phase and S/G 2 /M phases during cell division. FUCCI-expressing cancer cells (5 × 10 6 ) were placed on Gelfoam® (1 × 1 cm) in RPMI 1640 medium. ( A ) Cancer

    Article Snippet: FUCCI-expressing MKN45 cells were seeded on plastic plates for 2-dimensional culture in RPMI-1640 medium (Mediatech).

    Techniques: Migration, Expressing

    Figure 7.  Chemotherapy does not kill or inhibit the movement of single invading cancer cells in G 0 /G 1  phase. FUCCI-expressing cancer cells (2 × 10 6 ) were placed on Gelfoam® (1 × 1 cm) in RPMI 1640 medium. Three days after

    Journal: Cell Cycle

    Article Title: Invading cancer cells are predominantly in G0/G1 resulting in chemoresistance demonstrated by real-time FUCCI imaging

    doi: 10.4161/cc.27818

    Figure Lengend Snippet: Figure 7. Chemotherapy does not kill or inhibit the movement of single invading cancer cells in G 0 /G 1 phase. FUCCI-expressing cancer cells (2 × 10 6 ) were placed on Gelfoam® (1 × 1 cm) in RPMI 1640 medium. Three days after

    Article Snippet: FUCCI-expressing MKN45 cells were seeded on plastic plates for 2-dimensional culture in RPMI-1640 medium (Mediatech).

    Techniques: Expressing

    Figure 4.  Behavior of individual FUCCI-expressing cancer cells cultured on Gelfoam®. FUCCI-expressing cancer cells (5 × 10 6 ) were placed on Gelfoam® (1 × 1 cm) in RPMI 1640 medium. ( A ) Low-magnification image of

    Journal: Cell Cycle

    Article Title: Invading cancer cells are predominantly in G0/G1 resulting in chemoresistance demonstrated by real-time FUCCI imaging

    doi: 10.4161/cc.27818

    Figure Lengend Snippet: Figure 4. Behavior of individual FUCCI-expressing cancer cells cultured on Gelfoam®. FUCCI-expressing cancer cells (5 × 10 6 ) were placed on Gelfoam® (1 × 1 cm) in RPMI 1640 medium. ( A ) Low-magnification image of

    Article Snippet: FUCCI-expressing MKN45 cells were seeded on plastic plates for 2-dimensional culture in RPMI-1640 medium (Mediatech).

    Techniques: Expressing, Cell Culture

    Figure 2.  Cell cycle distribution of invasive and noninvasive cancer cells. FUCCI-expressing cancer cells (1 × 10 7 ) were placed on Gelfoam® (1 × 1 cm) in RPMI 1640 medium. ( A ) High-magnification real-time images of a small

    Journal: Cell Cycle

    Article Title: Invading cancer cells are predominantly in G0/G1 resulting in chemoresistance demonstrated by real-time FUCCI imaging

    doi: 10.4161/cc.27818

    Figure Lengend Snippet: Figure 2. Cell cycle distribution of invasive and noninvasive cancer cells. FUCCI-expressing cancer cells (1 × 10 7 ) were placed on Gelfoam® (1 × 1 cm) in RPMI 1640 medium. ( A ) High-magnification real-time images of a small

    Article Snippet: FUCCI-expressing MKN45 cells were seeded on plastic plates for 2-dimensional culture in RPMI-1640 medium (Mediatech).

    Techniques: Expressing

    Figure 3.  Invasive cancer cells are predominantly in G 0 /G 1 . FUCCI-expressing cancer cells (5 × 10 6 ) were placed on Gelfoam® (1 × 1 cm) in RPMI 1640 medium. ( A ) High-magnification real-time images of invading cancer cells

    Journal: Cell Cycle

    Article Title: Invading cancer cells are predominantly in G0/G1 resulting in chemoresistance demonstrated by real-time FUCCI imaging

    doi: 10.4161/cc.27818

    Figure Lengend Snippet: Figure 3. Invasive cancer cells are predominantly in G 0 /G 1 . FUCCI-expressing cancer cells (5 × 10 6 ) were placed on Gelfoam® (1 × 1 cm) in RPMI 1640 medium. ( A ) High-magnification real-time images of invading cancer cells

    Article Snippet: FUCCI-expressing MKN45 cells were seeded on plastic plates for 2-dimensional culture in RPMI-1640 medium (Mediatech).

    Techniques: Expressing