Journal: International Journal of Molecular Sciences

Article Title: Production of Spinocerebellar Ataxia Type 3 Model Mice by Intravenous Injection of AAV-PHP.B Vectors

doi: 10.3390/ijms25137205

Figure Lengend Snippet: Schema showing the experimental protocol. ( A ) Expression cassettes of the AAV genomes. The ubiquitous CBh promoter drives the expression of GFP-P2A-ATXN3(Q89) or -ATXN3(Q15). HA-tag was attached at the 5′-terminal of ATXN3 for immunolabeling the expressed ATXN3 protein by anti-HA antibody. ( B ) Schema showing experimental groups. Four-five-week wild-type mice were intravenously injected with high doses of BBB-permeable AAV-PHP.B encoding ATXN3(Q89), low-dose ATXN3(Q89) or high-dose ATXN3(Q15), or with PBS. ( C ) Diagram depicting the experimental protocol. A rotarod test was completed just before and every week up to 12 weeks after the AAV injection. After footprint analysis at 12 weeks, mice were sacrificed and subjected to immunohistochemistry (IHC).

Article Snippet: The motor coordination ability for SCA3 model mice was evaluated using a rotarod test (MK-610A/RKZ, Muromachi Kikai, Tokyo, Japan) and footprint analysis.

Techniques: Expressing, Immunolabeling, Injection, Immunohistochemistry

Journal: International Journal of Molecular Sciences

Article Title: Production of Spinocerebellar Ataxia Type 3 Model Mice by Intravenous Injection of AAV-PHP.B Vectors

doi: 10.3390/ijms25137205

Figure Lengend Snippet: Progressive motor deficits of mice injected with low doses of AAV-PHP.B encoding ATXN3(Q89). ( A ) Time course of rotarod performance of mice injected with AAV or PBS. Rotarod test was completed just before and every week post-injection. Mice were placed on a rod while accelerating from 4 to 40 rpm in 5 min, and the time it took for the mice to fall off the rod was measured. The average latency to fall of 4 trials was recorded. n = 7 [PBS, Q89(H)] or 8 [Q15, Q89(L)] mice, respectively. * p < 0.05, ** p < 0.01, *** p < 0.001 [vs. PBS], † p < 0.05, †† p < 0.01, ††† p < 0.001, †††† p < 0.0001 [vs. Q15(H)] by one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test at each time point. ( B , C ) Footprint analysis performed 12 weeks after AAV injection. Mice with their hind limbs painted with a blue dye walked on a white paper for 30 cm ( B ). The average stride length was measured, excluding the beginning and end of the footprints ( C ). n = 4 mice, respectively. **, †† p < 0.01 by one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test. n.s., not significant.

Article Snippet: The motor coordination ability for SCA3 model mice was evaluated using a rotarod test (MK-610A/RKZ, Muromachi Kikai, Tokyo, Japan) and footprint analysis.

Techniques: Injection

Journal: Frontiers in Pharmacology

Article Title: Ramipril Alleviates Oxaliplatin-Induced Acute Pain Syndrome in Mice

doi: 10.3389/fphar.2021.712442

Figure Lengend Snippet: Effects of OXP on motor performance and the heat nociceptive response.

Article Snippet: Motor coordination was assessed using the rotarod test (Bioseb, France) ( ).

Techniques: Hot Plate Test