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    Qiagen rneasey mini plus kit
    Representative gel electrophoresis of PCR reactions using universal bacterial primers and sputum <t>RNA</t> isolated with the <t>RNeasy</t> Plus Mini kit. Sputum cells were either subjected or not to bead vortexing (bashing or glass beads) prior to RNA isolation. Bands representing contaminating bacterial DNA are indicated. M: molecular weight marker.
    Rneasey Mini Plus Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 35861 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasey mini plus kit/product/Qiagen
    Average 99 stars, based on 35861 article reviews
    Price from $9.99 to $1999.99
    rneasey mini plus kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Qiagen rneasy mini plus kit
    Representative gel electrophoresis of PCR reactions using universal bacterial primers and sputum <t>RNA</t> isolated with the <t>RNeasy</t> Plus Mini kit. Sputum cells were either subjected or not to bead vortexing (bashing or glass beads) prior to RNA isolation. Bands representing contaminating bacterial DNA are indicated. M: molecular weight marker.
    Rneasy Mini Plus Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1681 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy mini plus kit/product/Qiagen
    Average 99 stars, based on 1681 article reviews
    Price from $9.99 to $1999.99
    rneasy mini plus kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Representative gel electrophoresis of PCR reactions using universal bacterial primers and sputum RNA isolated with the RNeasy Plus Mini kit. Sputum cells were either subjected or not to bead vortexing (bashing or glass beads) prior to RNA isolation. Bands representing contaminating bacterial DNA are indicated. M: molecular weight marker.

    Journal: PLoS ONE

    Article Title: Elimination of bacterial DNA during RNA isolation from sputum: Bashing bead vortexing is preferable over prolonged DNase treatment

    doi: 10.1371/journal.pone.0214609

    Figure Lengend Snippet: Representative gel electrophoresis of PCR reactions using universal bacterial primers and sputum RNA isolated with the RNeasy Plus Mini kit. Sputum cells were either subjected or not to bead vortexing (bashing or glass beads) prior to RNA isolation. Bands representing contaminating bacterial DNA are indicated. M: molecular weight marker.

    Article Snippet: Optimizing RNA isolation using the RNeasy Plus Mini kit The RNeasy Plus Mini kit offers two alternative protocols; the standard protocol provides enrichment of the sample with intact mRNAs by eliminating RNAs shorter than 200 nucleotides; the second protocol is designed for the purification of total RNA.

    Techniques: Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Isolation, Molecular Weight, Marker

    Cycle threshold (Ct) values for real-time PCR assays with different base pair (bp) long amplicons of glyceraldehyde 3-phophate dehydrogenase on RNA isolated from sputum samples. Samples were subjected either to repeated digestion process (up to 6 times) with the Turbo DNA-free kit or bead vortexing prior to RNA isolation using the RNeasy Plus Mini kit equipped with the gDNA eliminator spin column (n = 6 for each group). Error bars indicate SEM. # p

    Journal: PLoS ONE

    Article Title: Elimination of bacterial DNA during RNA isolation from sputum: Bashing bead vortexing is preferable over prolonged DNase treatment

    doi: 10.1371/journal.pone.0214609

    Figure Lengend Snippet: Cycle threshold (Ct) values for real-time PCR assays with different base pair (bp) long amplicons of glyceraldehyde 3-phophate dehydrogenase on RNA isolated from sputum samples. Samples were subjected either to repeated digestion process (up to 6 times) with the Turbo DNA-free kit or bead vortexing prior to RNA isolation using the RNeasy Plus Mini kit equipped with the gDNA eliminator spin column (n = 6 for each group). Error bars indicate SEM. # p

    Article Snippet: Optimizing RNA isolation using the RNeasy Plus Mini kit The RNeasy Plus Mini kit offers two alternative protocols; the standard protocol provides enrichment of the sample with intact mRNAs by eliminating RNAs shorter than 200 nucleotides; the second protocol is designed for the purification of total RNA.

    Techniques: Real-time Polymerase Chain Reaction, Isolation

    Replication Factors Are Misregulated in set2 Δ Cells (A) Transcript levels of cdc18 + , cdc22 + , and cdt1 + were established in exponentially growing wild-type and set2 Δ cells. RNA was extracted with Qiagen RNeasy kit and relative transcript levels of cdc18 + , cdc22 + , and cdt1 + were established by RT-qPCR. Error bars represent SD of three biological repeats. The asterisk ( ∗ ) represents significant difference compared with wild-type and set2 Δ. (B) Cdc18, Cdt1, or Cdc22 protein levels of exponentially growing wild-type and set2 Δ cells. Cell extracts were prepared from vegetative cells and processed for western blot. Immunoblots of total cell lysates were probed with PAP or GFP antibody. α-Tubulin is shown as a loading control. (C) cdc18-TAP or cdc18-TAP set2 Δ cells were arrested in G1 by nitrogen starvation and released, and samples were taken at time points indicated and subjected to FACS analysis. (D) In parallel, cdc18-TAP and cdc18-TAP set2 Δ cells were processed for western blotting at indicated times. (E) cdt1-TAP or cdt1-TAP set2 Δ cells were arrested in G1 by nitrogen starvation, released, and samples taken at time points indicated and subjected to FACS analysis. (F) In parallel, cdt1-TAP and cdt1-TAP set2 Δ cells were processed for western blotting at indicated times. (G) A similar experiment to that described in (C) was carried out using cdc22-CFP or cdc22-CFP set2 Δ cells. (H) A similar experiment to that described in (D) was carried out using cdc22-CFP or cdc22-CFP set2 Δ cells. Immunoblots of total cell extracts were probed with GFP antibody. α-Tubulin is shown as a loading control.

    Journal: Cell Reports

    Article Title: Set2 Methyltransferase Facilitates DNA Replication and Promotes Genotoxic Stress Responses through MBF-Dependent Transcription

    doi: 10.1016/j.celrep.2017.08.058

    Figure Lengend Snippet: Replication Factors Are Misregulated in set2 Δ Cells (A) Transcript levels of cdc18 + , cdc22 + , and cdt1 + were established in exponentially growing wild-type and set2 Δ cells. RNA was extracted with Qiagen RNeasy kit and relative transcript levels of cdc18 + , cdc22 + , and cdt1 + were established by RT-qPCR. Error bars represent SD of three biological repeats. The asterisk ( ∗ ) represents significant difference compared with wild-type and set2 Δ. (B) Cdc18, Cdt1, or Cdc22 protein levels of exponentially growing wild-type and set2 Δ cells. Cell extracts were prepared from vegetative cells and processed for western blot. Immunoblots of total cell lysates were probed with PAP or GFP antibody. α-Tubulin is shown as a loading control. (C) cdc18-TAP or cdc18-TAP set2 Δ cells were arrested in G1 by nitrogen starvation and released, and samples were taken at time points indicated and subjected to FACS analysis. (D) In parallel, cdc18-TAP and cdc18-TAP set2 Δ cells were processed for western blotting at indicated times. (E) cdt1-TAP or cdt1-TAP set2 Δ cells were arrested in G1 by nitrogen starvation, released, and samples taken at time points indicated and subjected to FACS analysis. (F) In parallel, cdt1-TAP and cdt1-TAP set2 Δ cells were processed for western blotting at indicated times. (G) A similar experiment to that described in (C) was carried out using cdc22-CFP or cdc22-CFP set2 Δ cells. (H) A similar experiment to that described in (D) was carried out using cdc22-CFP or cdc22-CFP set2 Δ cells. Immunoblots of total cell extracts were probed with GFP antibody. α-Tubulin is shown as a loading control.

    Article Snippet: Total RNA was prepared using the RNeasy Plus Kit (Qiagen) as indicated in the manufacturer’s manual.

    Techniques: Quantitative RT-PCR, Western Blot, FACS