rneasy plant mini kit Search Results


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  • 99
    Qiagen rneasay plant mini kit
    Conventional <t>PCR</t> to check amplicon size prior to multiplex PCR. Amplicon sizes were checked on two percent agarose gel prior to performing multiplex PCR for A. fumigatus dsRNA mycoviruses. AfuCV, AfuPV-1 and AfuTmV-1 dsRNAs were extracted using LiCl extraction (Lanes 2, 4 and 6, respectively) and <t>RNeasy</t> Plant Mini kit (Lanes 3, 5 and 7, respectively) and used as templates for amplification with AfuCV, AfuPV-1 and AfuTmV-1 primers (2–3, 4–5, 6–7, respectively). Hyperladder-I was used as a marker to estimate the size of the amplicons (Lane 1).
    Rneasay Plant Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 6832 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasay plant mini kit/product/Qiagen
    Average 99 stars, based on 6832 article reviews
    Price from $9.99 to $1999.99
    rneasay plant mini kit - by Bioz Stars, 2020-04
    99/100 stars
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    92
    Bioteke Corporation rneasy plant mini kit
    Conventional <t>PCR</t> to check amplicon size prior to multiplex PCR. Amplicon sizes were checked on two percent agarose gel prior to performing multiplex PCR for A. fumigatus dsRNA mycoviruses. AfuCV, AfuPV-1 and AfuTmV-1 dsRNAs were extracted using LiCl extraction (Lanes 2, 4 and 6, respectively) and <t>RNeasy</t> Plant Mini kit (Lanes 3, 5 and 7, respectively) and used as templates for amplification with AfuCV, AfuPV-1 and AfuTmV-1 primers (2–3, 4–5, 6–7, respectively). Hyperladder-I was used as a marker to estimate the size of the amplicons (Lane 1).
    Rneasy Plant Mini Kit, supplied by Bioteke Corporation, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy plant mini kit/product/Bioteke Corporation
    Average 92 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    rneasy plant mini kit - by Bioz Stars, 2020-04
    92/100 stars
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    94
    Thermo Fisher rneasy plant mini kit
    Conventional <t>PCR</t> to check amplicon size prior to multiplex PCR. Amplicon sizes were checked on two percent agarose gel prior to performing multiplex PCR for A. fumigatus dsRNA mycoviruses. AfuCV, AfuPV-1 and AfuTmV-1 dsRNAs were extracted using LiCl extraction (Lanes 2, 4 and 6, respectively) and <t>RNeasy</t> Plant Mini kit (Lanes 3, 5 and 7, respectively) and used as templates for amplification with AfuCV, AfuPV-1 and AfuTmV-1 primers (2–3, 4–5, 6–7, respectively). Hyperladder-I was used as a marker to estimate the size of the amplicons (Lane 1).
    Rneasy Plant Mini Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy plant mini kit/product/Thermo Fisher
    Average 94 stars, based on 133 article reviews
    Price from $9.99 to $1999.99
    rneasy plant mini kit - by Bioz Stars, 2020-04
    94/100 stars
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    96
    tiangen biotech co rneasy plant mini kit
    Conventional <t>PCR</t> to check amplicon size prior to multiplex PCR. Amplicon sizes were checked on two percent agarose gel prior to performing multiplex PCR for A. fumigatus dsRNA mycoviruses. AfuCV, AfuPV-1 and AfuTmV-1 dsRNAs were extracted using LiCl extraction (Lanes 2, 4 and 6, respectively) and <t>RNeasy</t> Plant Mini kit (Lanes 3, 5 and 7, respectively) and used as templates for amplification with AfuCV, AfuPV-1 and AfuTmV-1 primers (2–3, 4–5, 6–7, respectively). Hyperladder-I was used as a marker to estimate the size of the amplicons (Lane 1).
    Rneasy Plant Mini Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 96/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy plant mini kit/product/tiangen biotech co
    Average 96 stars, based on 46 article reviews
    Price from $9.99 to $1999.99
    rneasy plant mini kit - by Bioz Stars, 2020-04
    96/100 stars
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    93
    Millipore plant rneasy mini kit
    Conventional <t>PCR</t> to check amplicon size prior to multiplex PCR. Amplicon sizes were checked on two percent agarose gel prior to performing multiplex PCR for A. fumigatus dsRNA mycoviruses. AfuCV, AfuPV-1 and AfuTmV-1 dsRNAs were extracted using LiCl extraction (Lanes 2, 4 and 6, respectively) and <t>RNeasy</t> Plant Mini kit (Lanes 3, 5 and 7, respectively) and used as templates for amplification with AfuCV, AfuPV-1 and AfuTmV-1 primers (2–3, 4–5, 6–7, respectively). Hyperladder-I was used as a marker to estimate the size of the amplicons (Lane 1).
    Plant Rneasy Mini Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plant rneasy mini kit/product/Millipore
    Average 93 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    plant rneasy mini kit - by Bioz Stars, 2020-04
    93/100 stars
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    95
    Roboklon rneasy plant mini kit
    Conventional <t>PCR</t> to check amplicon size prior to multiplex PCR. Amplicon sizes were checked on two percent agarose gel prior to performing multiplex PCR for A. fumigatus dsRNA mycoviruses. AfuCV, AfuPV-1 and AfuTmV-1 dsRNAs were extracted using LiCl extraction (Lanes 2, 4 and 6, respectively) and <t>RNeasy</t> Plant Mini kit (Lanes 3, 5 and 7, respectively) and used as templates for amplification with AfuCV, AfuPV-1 and AfuTmV-1 primers (2–3, 4–5, 6–7, respectively). Hyperladder-I was used as a marker to estimate the size of the amplicons (Lane 1).
    Rneasy Plant Mini Kit, supplied by Roboklon, used in various techniques. Bioz Stars score: 95/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy plant mini kit/product/Roboklon
    Average 95 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    rneasy plant mini kit - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    93
    MACHEREY NAGEL rneasy plant mini kit
    Conventional <t>PCR</t> to check amplicon size prior to multiplex PCR. Amplicon sizes were checked on two percent agarose gel prior to performing multiplex PCR for A. fumigatus dsRNA mycoviruses. AfuCV, AfuPV-1 and AfuTmV-1 dsRNAs were extracted using LiCl extraction (Lanes 2, 4 and 6, respectively) and <t>RNeasy</t> Plant Mini kit (Lanes 3, 5 and 7, respectively) and used as templates for amplification with AfuCV, AfuPV-1 and AfuTmV-1 primers (2–3, 4–5, 6–7, respectively). Hyperladder-I was used as a marker to estimate the size of the amplicons (Lane 1).
    Rneasy Plant Mini Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy plant mini kit/product/MACHEREY NAGEL
    Average 93 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    rneasy plant mini kit - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    93
    Bio-Rad rneasy plant mini kit
    Conventional <t>PCR</t> to check amplicon size prior to multiplex PCR. Amplicon sizes were checked on two percent agarose gel prior to performing multiplex PCR for A. fumigatus dsRNA mycoviruses. AfuCV, AfuPV-1 and AfuTmV-1 dsRNAs were extracted using LiCl extraction (Lanes 2, 4 and 6, respectively) and <t>RNeasy</t> Plant Mini kit (Lanes 3, 5 and 7, respectively) and used as templates for amplification with AfuCV, AfuPV-1 and AfuTmV-1 primers (2–3, 4–5, 6–7, respectively). Hyperladder-I was used as a marker to estimate the size of the amplicons (Lane 1).
    Rneasy Plant Mini Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy plant mini kit/product/Bio-Rad
    Average 93 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    rneasy plant mini kit - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    95
    TaKaRa rneasy plant mini kit
    Conventional <t>PCR</t> to check amplicon size prior to multiplex PCR. Amplicon sizes were checked on two percent agarose gel prior to performing multiplex PCR for A. fumigatus dsRNA mycoviruses. AfuCV, AfuPV-1 and AfuTmV-1 dsRNAs were extracted using LiCl extraction (Lanes 2, 4 and 6, respectively) and <t>RNeasy</t> Plant Mini kit (Lanes 3, 5 and 7, respectively) and used as templates for amplification with AfuCV, AfuPV-1 and AfuTmV-1 primers (2–3, 4–5, 6–7, respectively). Hyperladder-I was used as a marker to estimate the size of the amplicons (Lane 1).
    Rneasy Plant Mini Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy plant mini kit/product/TaKaRa
    Average 95 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    rneasy plant mini kit - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    Image Search Results


    Conventional PCR to check amplicon size prior to multiplex PCR. Amplicon sizes were checked on two percent agarose gel prior to performing multiplex PCR for A. fumigatus dsRNA mycoviruses. AfuCV, AfuPV-1 and AfuTmV-1 dsRNAs were extracted using LiCl extraction (Lanes 2, 4 and 6, respectively) and RNeasy Plant Mini kit (Lanes 3, 5 and 7, respectively) and used as templates for amplification with AfuCV, AfuPV-1 and AfuTmV-1 primers (2–3, 4–5, 6–7, respectively). Hyperladder-I was used as a marker to estimate the size of the amplicons (Lane 1).

    Journal: Viruses

    Article Title: Multiplex Detection of Aspergillus fumigatus Mycoviruses

    doi: 10.3390/v10050247

    Figure Lengend Snippet: Conventional PCR to check amplicon size prior to multiplex PCR. Amplicon sizes were checked on two percent agarose gel prior to performing multiplex PCR for A. fumigatus dsRNA mycoviruses. AfuCV, AfuPV-1 and AfuTmV-1 dsRNAs were extracted using LiCl extraction (Lanes 2, 4 and 6, respectively) and RNeasy Plant Mini kit (Lanes 3, 5 and 7, respectively) and used as templates for amplification with AfuCV, AfuPV-1 and AfuTmV-1 primers (2–3, 4–5, 6–7, respectively). Hyperladder-I was used as a marker to estimate the size of the amplicons (Lane 1).

    Article Snippet: The effect of the dsRNA extraction method on the efficiency of PCR was tested using LiCl extraction and the RNeasy Plant Mini kit (Qiagen).

    Techniques: Polymerase Chain Reaction, Amplification, Multiplex Assay, Agarose Gel Electrophoresis, Marker

    Total RNA integrity test on 1.0% agarose gel. Intact 28S and 18S total RNA bands can be observed on the agarose gel, indicating a good integrity of the total RNA after being extracted by different methods (lanes 1 and 2: modified CTAB method, lane 3, 4: RNAzol RT, and lane 5, 6: RNeasy Plant Mini kit).

    Journal: BioMed Research International

    Article Title: Molecular Cloning and Characterization of Novel Phytocystatin Gene from Turmeric, Curcuma longa

    doi: 10.1155/2014/973790

    Figure Lengend Snippet: Total RNA integrity test on 1.0% agarose gel. Intact 28S and 18S total RNA bands can be observed on the agarose gel, indicating a good integrity of the total RNA after being extracted by different methods (lanes 1 and 2: modified CTAB method, lane 3, 4: RNAzol RT, and lane 5, 6: RNeasy Plant Mini kit).

    Article Snippet: However in terms of the speed of extraction, RNAzol RT and Qiagen RNeasy Plant Mini kits were fast and able to extract total RNA in less than an hour whereas CTAB method required 3 days to achieve the same result.

    Techniques: Agarose Gel Electrophoresis, Modification

    The efficiency of filter paper for purification of nucleic acids from various sources using respective Qiagen kits. (A) Tomato genomic DNAs purified using Qiagen DNeasy plant mini kit. (B) Tomato total RNAs purified using Qiagen RNeasy plant mini kit. (C) PCR products of a GUS fragment purified using Qiagen QIAquick PCR purification kit. (D) PCR products of GUS fragment recovered from an agarose gel using a Qiagen QIAquick gel extraction kit. (E) pUC -19 plasmid DNAs purified using a Qiagen QIAprep spin miniprep kit. For each panel, from left to right are (Q) nucleic acid purified in experiments using original Qiagen spin column, (G) reassembled spin column using two layers of Whatman glass microfiber filters (Grade GF/F), and (P) reassembled spin column using two layers of Whatman qualitative filter paper, (Grade 3) respectively. Upper panel is quantification data based on three experimental replicates normalized according to performance of the Qiagen kit; lower panel is an image of agarose gel electrophoresis for the same volume of purified nucleic acids.

    Journal: PLoS ONE

    Article Title: Filter paper-based spin column method for cost-efficient DNA or RNA purification

    doi: 10.1371/journal.pone.0203011

    Figure Lengend Snippet: The efficiency of filter paper for purification of nucleic acids from various sources using respective Qiagen kits. (A) Tomato genomic DNAs purified using Qiagen DNeasy plant mini kit. (B) Tomato total RNAs purified using Qiagen RNeasy plant mini kit. (C) PCR products of a GUS fragment purified using Qiagen QIAquick PCR purification kit. (D) PCR products of GUS fragment recovered from an agarose gel using a Qiagen QIAquick gel extraction kit. (E) pUC -19 plasmid DNAs purified using a Qiagen QIAprep spin miniprep kit. For each panel, from left to right are (Q) nucleic acid purified in experiments using original Qiagen spin column, (G) reassembled spin column using two layers of Whatman glass microfiber filters (Grade GF/F), and (P) reassembled spin column using two layers of Whatman qualitative filter paper, (Grade 3) respectively. Upper panel is quantification data based on three experimental replicates normalized according to performance of the Qiagen kit; lower panel is an image of agarose gel electrophoresis for the same volume of purified nucleic acids.

    Article Snippet: Purification of tobacco total RNA using a filter paper-based spin column with homemade buffers is similar, or superior, to the performance of the Qiagen Plant RNeasy mini kit ( , ).

    Techniques: Purification, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Gel Extraction, Plasmid Preparation

    Evaluation of purification of tobacco genomic DNA and total RNA using filter paper-based spin columns with respective Qiagen kit buffers and homemade buffers. (A) Agarose gel electrophoresis for 2.5 μl tobacco genomic DNAs elution from purification experiments using Qiagen DNeasy plant mini kit buffers with Qiagen original spin column (Lane Q/Q), filter paper recharged used spin column (Lane Q/R) and filter paper-based homemade spin column (Lane Q/H*), followed by tobacco genomic DNAs purified using homemade buffer with Qiagen original spin column (Lane H/Q), filter paper recharged used spin column (Lane H/R) and filter paper-based homemade spin column (Lane H/H*). (B) UV spectrum curve of tobacco DNAs purified using Qiagen kit (Q/Q, black curve), filter paper recharged spin columns with Qiagen kit buffers (Q/R, blue curve) or homemade buffers (H/R, red curve) from the same amount leaf tissue. Y-axis is UV absorbance, and X-axis is wavelength (nM). (C) Amplification plots for three duplicated qPCR reactions contain 20 ng DNA purified using Qiagen kit (Q/Q, Blue curves) or DNA purified from filter paper recharged spin column with homemade buffer (H/R, Red curves) respectively. The x-axis is PCR cycle numbers, Y-axis is the level of SYBR fluorescence, and the green line is an arbitrary threshold to determine the Cq value (the fractional cycle number at which amplification curve meet threshold level). (D) MOPS-formaldehyde denaturing agarose gel electrophoresis separated 5 μl RNA purified using Qiagen RNeasy plant mini kit buffers with a Qiagen original spin column (Lane Q/Q), filter paper recharged used spin column (Lane Q/R) and homemade filter paper-based spin column (Lane Q/H*), followed total tobacco RNAs purified by using homemade buffer with Qiagen original spin column (Lane H/Q), filter paper recharged used spin column (Lane H/R) and filter paper-based homemade spin column (Lane H/H*). (E) UV spectrum of tobacco total RNA purified using Qiagen kit (Q/Q, black curve), filter paper recharged spin column with Qiagen RNeasy plant mini kit buffers (Q/R, blue curve) or homemade buffers (H/R, red curve). Y-axis is UV absorbance, and the X-axis is wavelength. (F) Amplification plots of three duplicated qRT-PCR reactions for 2.5 ng RNA purified using Qiagen kit (Q/Q, Blue curves) or RNA purified using filter paper recharged spin column with homemade buffer (H/R, Red curves) respectively. Note: * The starting material amount is 100 mg tobacco leaf tissue for experiments using a Qiagen spin column or filter paper recharged spin column, and half amount of plant sample (50 mg) used for homemade spin column purification. All DNAs or RNAs were eluted using 100 ul elution solution.

    Journal: PLoS ONE

    Article Title: Filter paper-based spin column method for cost-efficient DNA or RNA purification

    doi: 10.1371/journal.pone.0203011

    Figure Lengend Snippet: Evaluation of purification of tobacco genomic DNA and total RNA using filter paper-based spin columns with respective Qiagen kit buffers and homemade buffers. (A) Agarose gel electrophoresis for 2.5 μl tobacco genomic DNAs elution from purification experiments using Qiagen DNeasy plant mini kit buffers with Qiagen original spin column (Lane Q/Q), filter paper recharged used spin column (Lane Q/R) and filter paper-based homemade spin column (Lane Q/H*), followed by tobacco genomic DNAs purified using homemade buffer with Qiagen original spin column (Lane H/Q), filter paper recharged used spin column (Lane H/R) and filter paper-based homemade spin column (Lane H/H*). (B) UV spectrum curve of tobacco DNAs purified using Qiagen kit (Q/Q, black curve), filter paper recharged spin columns with Qiagen kit buffers (Q/R, blue curve) or homemade buffers (H/R, red curve) from the same amount leaf tissue. Y-axis is UV absorbance, and X-axis is wavelength (nM). (C) Amplification plots for three duplicated qPCR reactions contain 20 ng DNA purified using Qiagen kit (Q/Q, Blue curves) or DNA purified from filter paper recharged spin column with homemade buffer (H/R, Red curves) respectively. The x-axis is PCR cycle numbers, Y-axis is the level of SYBR fluorescence, and the green line is an arbitrary threshold to determine the Cq value (the fractional cycle number at which amplification curve meet threshold level). (D) MOPS-formaldehyde denaturing agarose gel electrophoresis separated 5 μl RNA purified using Qiagen RNeasy plant mini kit buffers with a Qiagen original spin column (Lane Q/Q), filter paper recharged used spin column (Lane Q/R) and homemade filter paper-based spin column (Lane Q/H*), followed total tobacco RNAs purified by using homemade buffer with Qiagen original spin column (Lane H/Q), filter paper recharged used spin column (Lane H/R) and filter paper-based homemade spin column (Lane H/H*). (E) UV spectrum of tobacco total RNA purified using Qiagen kit (Q/Q, black curve), filter paper recharged spin column with Qiagen RNeasy plant mini kit buffers (Q/R, blue curve) or homemade buffers (H/R, red curve). Y-axis is UV absorbance, and the X-axis is wavelength. (F) Amplification plots of three duplicated qRT-PCR reactions for 2.5 ng RNA purified using Qiagen kit (Q/Q, Blue curves) or RNA purified using filter paper recharged spin column with homemade buffer (H/R, Red curves) respectively. Note: * The starting material amount is 100 mg tobacco leaf tissue for experiments using a Qiagen spin column or filter paper recharged spin column, and half amount of plant sample (50 mg) used for homemade spin column purification. All DNAs or RNAs were eluted using 100 ul elution solution.

    Article Snippet: Purification of tobacco total RNA using a filter paper-based spin column with homemade buffers is similar, or superior, to the performance of the Qiagen Plant RNeasy mini kit ( , ).

    Techniques: Purification, Agarose Gel Electrophoresis, Amplification, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Fluorescence, Quantitative RT-PCR

    Representative end-point RT-PCR gene expression results for (a) 18S and (b) TfR from RNA isolated using the freeze grind+CTAB+RNeasy ® (FCR) method, the mince+CTAB+RNeasy ® (MCR) method, and the lysozyme+CTAB+RNeasy ® (LCR) method. Genomic contamination was detected following agarose gel electrophoresis in all minus-RT controls. In addition, as shown in the TfR results, where the primers were designed to span an intron–exon boundary, two products were formed during the PCR, corresponding to a genomic product size of 270 bp and an mRNA product size of 62 bp.

    Journal: Tissue Engineering. Part C, Methods

    Article Title: Techniques for the Isolation of High-Quality RNA from Cells Encapsulated in Chitosan Hydrogels

    doi: 10.1089/ten.tec.2012.0693

    Figure Lengend Snippet: Representative end-point RT-PCR gene expression results for (a) 18S and (b) TfR from RNA isolated using the freeze grind+CTAB+RNeasy ® (FCR) method, the mince+CTAB+RNeasy ® (MCR) method, and the lysozyme+CTAB+RNeasy ® (LCR) method. Genomic contamination was detected following agarose gel electrophoresis in all minus-RT controls. In addition, as shown in the TfR results, where the primers were designed to span an intron–exon boundary, two products were formed during the PCR, corresponding to a genomic product size of 270 bp and an mRNA product size of 62 bp.

    Article Snippet: Commercially available RNA extraction kits such as the Qiagen RNeasy® Mini Plant kit or the RNeasy® Mini kit have also been used as methods for extracting RNA from agarose and gellan, as well as alginate-based scaffolds., In general, most of these methods initially involve a form of mechanical disruption to help separate the RNA.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Isolation, Agarose Gel Electrophoresis, Polymerase Chain Reaction

    Relative expression levels of 9 stress-related genes in the WT and OsABA8ox3 RNAi transgenic plants under normal and PEG-treated conditions detected by qRT-PCR. Four-leaf stage seedlings were treated with 20% PEG for 2 h, and then the samples were quickly frozen with liquid nitrogen for RNA extraction. The genes include dehydration-responsive element-binding protein (Os06g03670), zinc finger protein (Os05g10670), late embryogenesis abundant proteins (Os05g46480, Os01g50910 and Os04g49980), dehydrin family proteins (Os11g26760 and Os01g50700) and heat shock protein (Os03g16920 and Os02g54140).

    Journal: PLoS ONE

    Article Title: A Key ABA Catabolic Gene, OsABA8ox3, Is Involved in Drought Stress Resistance in Rice

    doi: 10.1371/journal.pone.0116646

    Figure Lengend Snippet: Relative expression levels of 9 stress-related genes in the WT and OsABA8ox3 RNAi transgenic plants under normal and PEG-treated conditions detected by qRT-PCR. Four-leaf stage seedlings were treated with 20% PEG for 2 h, and then the samples were quickly frozen with liquid nitrogen for RNA extraction. The genes include dehydration-responsive element-binding protein (Os06g03670), zinc finger protein (Os05g10670), late embryogenesis abundant proteins (Os05g46480, Os01g50910 and Os04g49980), dehydrin family proteins (Os11g26760 and Os01g50700) and heat shock protein (Os03g16920 and Os02g54140).

    Article Snippet: Gene expression analysis by quantitative real-time PCR (qRT-PCR) Total RNA was extracted from rice seedlings using an RNA Easy Plant Mini Kit (Qiagen, CA) and then digested with DNase I (Amersham, USA) to eliminate genomic DNA contamination.

    Techniques: Expressing, Transgenic Assay, Quantitative RT-PCR, RNA Extraction, Binding Assay

    Agarose gel electrophoresis and the respective electropherograms of the total RNA samples extracted from (a) leaves, and (b) stems, of Stevia rebaudiana . Two distinct rRNA bands and peaks were clearly observed, for each sample, in both the gel and electropherograms respectively, signifying high RNA quality and minimal degradation. LT1-LT3: Replicates of RNA extracts from leaf tissues (400–600 ng); ST1-ST3: Replicates of RNA extracts from stem tissues (300–400 ng).

    Journal: Tropical Life Sciences Research

    Article Title: Efficient and High-Quality RNA Isolation from Metabolite-Rich Tissues of Stevia rebaudiana, an Important Commercial Crop

    doi: 10.21315/tlsr2019.30.1.9

    Figure Lengend Snippet: Agarose gel electrophoresis and the respective electropherograms of the total RNA samples extracted from (a) leaves, and (b) stems, of Stevia rebaudiana . Two distinct rRNA bands and peaks were clearly observed, for each sample, in both the gel and electropherograms respectively, signifying high RNA quality and minimal degradation. LT1-LT3: Replicates of RNA extracts from leaf tissues (400–600 ng); ST1-ST3: Replicates of RNA extracts from stem tissues (300–400 ng).

    Article Snippet: Comparison with Previously-Reported RNA Extraction Methods from Stevia rebaudiana Commercially-available kits, such as Qiagen RNA plant mini kit (Qiagen, Hilden, Germany) and Spectrum Plant Total RNA Kit (Sigma-Aldrich, Missouri, USA), had been successfully used to isolate RNA from the leaf tissues of Stevia rebaudiana ( ; ).

    Techniques: Agarose Gel Electrophoresis

    RNA separation on 1% agarose gel . A : RNA extracted from Arabidopsis leaves. Lane 1, RNA extracted using Qiagen RNeasy kit (kit) and lane 2, RNA extracted by LogSpin protocol. B : RNA extracted from B. cinerea (Botrytis) or A. brassicicola (Alternaria) mycelium. Lanes 1 and 3, RNA extracted using Norgen Plant/Fungi RNA Purification kit (kit). Lanes 2 and 4, RNA extracted using LogSpin protocol. C : Qualitative assessment of the integrity of a total RNA sample extracted using LogSpin protocol from tomato leaves by bioanalyzer. M (bp), DNA ladder in base pairs; kit, gel provided by the bioanalyzer kit.

    Journal: BMC Research Notes

    Article Title: LogSpin: a simple, economical and fast method for RNA isolation from infected or healthy plants and other eukaryotic tissues

    doi: 10.1186/1756-0500-5-45

    Figure Lengend Snippet: RNA separation on 1% agarose gel . A : RNA extracted from Arabidopsis leaves. Lane 1, RNA extracted using Qiagen RNeasy kit (kit) and lane 2, RNA extracted by LogSpin protocol. B : RNA extracted from B. cinerea (Botrytis) or A. brassicicola (Alternaria) mycelium. Lanes 1 and 3, RNA extracted using Norgen Plant/Fungi RNA Purification kit (kit). Lanes 2 and 4, RNA extracted using LogSpin protocol. C : Qualitative assessment of the integrity of a total RNA sample extracted using LogSpin protocol from tomato leaves by bioanalyzer. M (bp), DNA ladder in base pairs; kit, gel provided by the bioanalyzer kit.

    Article Snippet: The use of a plasmid DNA extraction column as an RNA-binding device for RNA extraction was first tested by comparing plasmid DNA extraction spin columns (QIAprep Spin Miniprep Kit, cat. no. 27106, Qiagen, Hilden, Germany) with RNA extraction spin columns from the commercial plant RNA extraction kit RNeasy (RNeasy Plant Mini Kit, Qiagen, cat. no. 74904).

    Techniques: Agarose Gel Electrophoresis, Purification