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  • 92
    Thermo Fisher qiagen rneasy minikit
    Qiagen Rneasy Minikit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiagen rneasy minikit/product/Thermo Fisher
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    qiagen rneasy minikit - by Bioz Stars, 2020-07
    92/100 stars
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    99
    Qiagen qiagen rneasy minikit
    Validation of gene silencing in HEp-2C and Vero cells by quantitative PCR (qPCR). siRNAs targeting selected hit genes were transfected into HEp-2C or Vero cells. At 48 h posttransfection, cells were harvested for RNA isolation using a Qiagen <t>RNeasy</t> <t>minikit.</t>
    Qiagen Rneasy Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 5649 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiagen rneasy minikit/product/Qiagen
    Average 99 stars, based on 5649 article reviews
    Price from $9.99 to $1999.99
    qiagen rneasy minikit - by Bioz Stars, 2020-07
    99/100 stars
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    84
    Qiagen pn10 ovaries qiagen rneasy minikit
    Validation of gene silencing in HEp-2C and Vero cells by quantitative PCR (qPCR). siRNAs targeting selected hit genes were transfected into HEp-2C or Vero cells. At 48 h posttransfection, cells were harvested for RNA isolation using a Qiagen <t>RNeasy</t> <t>minikit.</t>
    Pn10 Ovaries Qiagen Rneasy Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pn10 ovaries qiagen rneasy minikit/product/Qiagen
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pn10 ovaries qiagen rneasy minikit - by Bioz Stars, 2020-07
    84/100 stars
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    99
    Qiagen qiagen easy minikit
    Validation of gene silencing in HEp-2C and Vero cells by quantitative PCR (qPCR). siRNAs targeting selected hit genes were transfected into HEp-2C or Vero cells. At 48 h posttransfection, cells were harvested for RNA isolation using a Qiagen <t>RNeasy</t> <t>minikit.</t>
    Qiagen Easy Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 202 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiagen easy minikit/product/Qiagen
    Average 99 stars, based on 202 article reviews
    Price from $9.99 to $1999.99
    qiagen easy minikit - by Bioz Stars, 2020-07
    99/100 stars
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    99
    Qiagen rneasy plant minikit
    Autoradiogram of 5% PAGE analysis of self-cleavage experiments using either 2′,5′- or 3′,5′-C-PLMVd. Radioactive transcripts (C-PLMVd) were added to the ground leaf powder, and the RNA was extracted using the <t>RNeasy</t> plant <t>minikit</t> and incubated under self-cleavage conditions with or without prior heat denaturation and snap-cooling treatments. Lanes 1 and 6, untreated L-PLMVd (control); lanes 2 to 5 and 7 to 10, 3′,5′- and 2′,5′-C-PLMVd, respectively; lanes 2 and 7, C-PLMVd extracted and incubated under self-cleavage conditions without heat denaturation and snap-cooling steps; lanes 3 and 8, like lanes 2 and 7, except that the extraction was performed in the presence of additional 5 mM EDTA in all buffers; lanes 4 and 9, like lanes 2 and 7, except that the samples were heat denatured and snap-cooled prior to incubation under self-cleavage conditions; lanes 5 and 10, like lanes 4 and 9, except that the extraction was performed in the presence of additional 5 mM EDTA. Adjacent to the gel, the positions of the C-PLMVd and L-PLMVd transcripts are used as size references.
    Rneasy Plant Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1675 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy plant minikit/product/Qiagen
    Average 99 stars, based on 1675 article reviews
    Price from $9.99 to $1999.99
    rneasy plant minikit - by Bioz Stars, 2020-07
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    99
    Qiagen rneasy protect minikits
    Autoradiogram of 5% PAGE analysis of self-cleavage experiments using either 2′,5′- or 3′,5′-C-PLMVd. Radioactive transcripts (C-PLMVd) were added to the ground leaf powder, and the RNA was extracted using the <t>RNeasy</t> plant <t>minikit</t> and incubated under self-cleavage conditions with or without prior heat denaturation and snap-cooling treatments. Lanes 1 and 6, untreated L-PLMVd (control); lanes 2 to 5 and 7 to 10, 3′,5′- and 2′,5′-C-PLMVd, respectively; lanes 2 and 7, C-PLMVd extracted and incubated under self-cleavage conditions without heat denaturation and snap-cooling steps; lanes 3 and 8, like lanes 2 and 7, except that the extraction was performed in the presence of additional 5 mM EDTA in all buffers; lanes 4 and 9, like lanes 2 and 7, except that the samples were heat denatured and snap-cooled prior to incubation under self-cleavage conditions; lanes 5 and 10, like lanes 4 and 9, except that the extraction was performed in the presence of additional 5 mM EDTA. Adjacent to the gel, the positions of the C-PLMVd and L-PLMVd transcripts are used as size references.
    Rneasy Protect Minikits, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy protect minikits/product/Qiagen
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    rneasy protect minikits - by Bioz Stars, 2020-07
    99/100 stars
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    99
    Qiagen rneasy lipid tissue minikit
    Autoradiogram of 5% PAGE analysis of self-cleavage experiments using either 2′,5′- or 3′,5′-C-PLMVd. Radioactive transcripts (C-PLMVd) were added to the ground leaf powder, and the RNA was extracted using the <t>RNeasy</t> plant <t>minikit</t> and incubated under self-cleavage conditions with or without prior heat denaturation and snap-cooling treatments. Lanes 1 and 6, untreated L-PLMVd (control); lanes 2 to 5 and 7 to 10, 3′,5′- and 2′,5′-C-PLMVd, respectively; lanes 2 and 7, C-PLMVd extracted and incubated under self-cleavage conditions without heat denaturation and snap-cooling steps; lanes 3 and 8, like lanes 2 and 7, except that the extraction was performed in the presence of additional 5 mM EDTA in all buffers; lanes 4 and 9, like lanes 2 and 7, except that the samples were heat denatured and snap-cooled prior to incubation under self-cleavage conditions; lanes 5 and 10, like lanes 4 and 9, except that the extraction was performed in the presence of additional 5 mM EDTA. Adjacent to the gel, the positions of the C-PLMVd and L-PLMVd transcripts are used as size references.
    Rneasy Lipid Tissue Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 336 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy lipid tissue minikit/product/Qiagen
    Average 99 stars, based on 336 article reviews
    Price from $9.99 to $1999.99
    rneasy lipid tissue minikit - by Bioz Stars, 2020-07
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    Image Search Results


    Validation of gene silencing in HEp-2C and Vero cells by quantitative PCR (qPCR). siRNAs targeting selected hit genes were transfected into HEp-2C or Vero cells. At 48 h posttransfection, cells were harvested for RNA isolation using a Qiagen RNeasy minikit.

    Journal: Journal of Virology

    Article Title: Engineering Enhanced Vaccine Cell Lines To Eradicate Vaccine-Preventable Diseases: the Polio End Game

    doi: 10.1128/JVI.01464-15

    Figure Lengend Snippet: Validation of gene silencing in HEp-2C and Vero cells by quantitative PCR (qPCR). siRNAs targeting selected hit genes were transfected into HEp-2C or Vero cells. At 48 h posttransfection, cells were harvested for RNA isolation using a Qiagen RNeasy minikit.

    Article Snippet: Forty-eight hours after siRNA transfection, total RNA was isolated from mock-infected and transfected HEp-2C cells and Vero cells using an RNeasy minikit (Qiagen) according to the manufacturer's protocol.

    Techniques: Real-time Polymerase Chain Reaction, Transfection, Isolation

    Autoradiogram of 5% PAGE analysis of self-cleavage experiments using either 2′,5′- or 3′,5′-C-PLMVd. Radioactive transcripts (C-PLMVd) were added to the ground leaf powder, and the RNA was extracted using the RNeasy plant minikit and incubated under self-cleavage conditions with or without prior heat denaturation and snap-cooling treatments. Lanes 1 and 6, untreated L-PLMVd (control); lanes 2 to 5 and 7 to 10, 3′,5′- and 2′,5′-C-PLMVd, respectively; lanes 2 and 7, C-PLMVd extracted and incubated under self-cleavage conditions without heat denaturation and snap-cooling steps; lanes 3 and 8, like lanes 2 and 7, except that the extraction was performed in the presence of additional 5 mM EDTA in all buffers; lanes 4 and 9, like lanes 2 and 7, except that the samples were heat denatured and snap-cooled prior to incubation under self-cleavage conditions; lanes 5 and 10, like lanes 4 and 9, except that the extraction was performed in the presence of additional 5 mM EDTA. Adjacent to the gel, the positions of the C-PLMVd and L-PLMVd transcripts are used as size references.

    Journal: Journal of Virology

    Article Title: Natural 2?,5?-Phosphodiester Bonds Found at the Ligation Sites of Peach Latent Mosaic Viroid

    doi: 10.1128/JVI.75.1.19-25.2001

    Figure Lengend Snippet: Autoradiogram of 5% PAGE analysis of self-cleavage experiments using either 2′,5′- or 3′,5′-C-PLMVd. Radioactive transcripts (C-PLMVd) were added to the ground leaf powder, and the RNA was extracted using the RNeasy plant minikit and incubated under self-cleavage conditions with or without prior heat denaturation and snap-cooling treatments. Lanes 1 and 6, untreated L-PLMVd (control); lanes 2 to 5 and 7 to 10, 3′,5′- and 2′,5′-C-PLMVd, respectively; lanes 2 and 7, C-PLMVd extracted and incubated under self-cleavage conditions without heat denaturation and snap-cooling steps; lanes 3 and 8, like lanes 2 and 7, except that the extraction was performed in the presence of additional 5 mM EDTA in all buffers; lanes 4 and 9, like lanes 2 and 7, except that the samples were heat denatured and snap-cooled prior to incubation under self-cleavage conditions; lanes 5 and 10, like lanes 4 and 9, except that the extraction was performed in the presence of additional 5 mM EDTA. Adjacent to the gel, the positions of the C-PLMVd and L-PLMVd transcripts are used as size references.

    Article Snippet: The first procedure used an RNeasy plant minikit (Qiagen) to prepare RNA from 50 to 100 mg of tissue as specified by the manufacturer.

    Techniques: Polyacrylamide Gel Electrophoresis, Incubation