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    Qiagen rneasy mini kit rneasy mini
    Gel electrophoresis of total <t>RNA</t> that was obtained from sessile cells with sheared whole-cell lysis coupled to the <t>RNeasy</t> Mini Kit. Lanes 1 , 2 , and 3 represent three independent experiments using the methicillin-resistant S. aureus (MRSA) isolate BMB9393 ( A ) and the methicillin-susceptible S. aureus (MSSA) isolate HC474 ( B ).
    Rneasy Mini Kit Rneasy Mini, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 17577 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy mini kit rneasy mini/product/Qiagen
    Average 99 stars, based on 17577 article reviews
    Price from $9.99 to $1999.99
    rneasy mini kit rneasy mini - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Qiagen rneasy plus mini kit
    Representative gel electrophoresis of PCR reactions using universal bacterial primers and sputum <t>RNA</t> isolated with the <t>RNeasy</t> Plus Mini kit. Sputum cells were either subjected or not to bead vortexing (bashing or glass beads) prior to RNA isolation. Bands representing contaminating bacterial DNA are indicated. M: molecular weight marker.
    Rneasy Plus Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1267 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy plus mini kit/product/Qiagen
    Average 99 stars, based on 1267 article reviews
    Price from $9.99 to $1999.99
    rneasy plus mini kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Gel electrophoresis of total RNA that was obtained from sessile cells with sheared whole-cell lysis coupled to the RNeasy Mini Kit. Lanes 1 , 2 , and 3 represent three independent experiments using the methicillin-resistant S. aureus (MRSA) isolate BMB9393 ( A ) and the methicillin-susceptible S. aureus (MSSA) isolate HC474 ( B ).

    Journal: Brazilian Journal of Medical and Biological Research

    Article Title: Optimization of the RNeasy Mini Kit to obtain high-quality total RNA from sessile cells of Staphylococcus aureus

    doi: 10.1590/1414-431X20154734

    Figure Lengend Snippet: Gel electrophoresis of total RNA that was obtained from sessile cells with sheared whole-cell lysis coupled to the RNeasy Mini Kit. Lanes 1 , 2 , and 3 represent three independent experiments using the methicillin-resistant S. aureus (MRSA) isolate BMB9393 ( A ) and the methicillin-susceptible S. aureus (MSSA) isolate HC474 ( B ).

    Article Snippet: Therefore, we designed a simple optimized protocol using the RNeasy Mini Kit that ensures high-quality RNA preparations from planktonic and sessile cells of MRSA, compatible with experiments that require integrity and good quantity of this molecule.

    Techniques: Nucleic Acid Electrophoresis, Lysis

    CD63 mRNA down regulation by siRNA in human MDMs which are challenged with different concentration of HIV-1. A. MDMs (5 × 10 5 cells/well) were plated in 24-well plates and transfected with 50 nM siRNAs (CD63, CD4, and ERBB2IP). For controls, cells were treated with AZT (1 mM) or raltegravir (20 mM). Controls also included untreated cells or cells infected with HIV-1 SX (m.o.i. = 0.02) only. Total mRNA was isolated from each well using the Qiagen RNeasy kit 48 h after transfection. Quantitative RT-PCR was used to determine relative CD63 expression levels, normalizing to GAPDH expression as an internal control. B. MDMs were challenged with different concentration of HIV-1 SX (m.o.i. = 0.6, 0.2. 0.06 and 0.02, respectively). Supernatants were harvested for p24 detection on day 7 post-infection for MDMs using p24 Capture ELISA kit (ImmunoDiagnostics, Woburn, MA).

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Tetraspanin CD63 is a regulator of HIV-1 replication

    doi:

    Figure Lengend Snippet: CD63 mRNA down regulation by siRNA in human MDMs which are challenged with different concentration of HIV-1. A. MDMs (5 × 10 5 cells/well) were plated in 24-well plates and transfected with 50 nM siRNAs (CD63, CD4, and ERBB2IP). For controls, cells were treated with AZT (1 mM) or raltegravir (20 mM). Controls also included untreated cells or cells infected with HIV-1 SX (m.o.i. = 0.02) only. Total mRNA was isolated from each well using the Qiagen RNeasy kit 48 h after transfection. Quantitative RT-PCR was used to determine relative CD63 expression levels, normalizing to GAPDH expression as an internal control. B. MDMs were challenged with different concentration of HIV-1 SX (m.o.i. = 0.6, 0.2. 0.06 and 0.02, respectively). Supernatants were harvested for p24 detection on day 7 post-infection for MDMs using p24 Capture ELISA kit (ImmunoDiagnostics, Woburn, MA).

    Article Snippet: Total mRNA was isolated from siRNA-transfected cells and MDMs using RNeasy Mini Kits (Qiagen).

    Techniques: Concentration Assay, Transfection, Infection, Isolation, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay

    Effects of CD63 silencing on HIV-1 replication in human PBLs and DCs. A. PBLs (1 × 10 5 cells/well) were plated in triplicate in 24-well plates and transfected with 50 nM siRNAs (CD63, CD4, and ERBB2IP). Controls included untreated cells or cells treated with virus infection only. Total mRNA was isolated from each well using the Qiagen RNeasy kit 48 h after transfection. Quantitative RT-PCR was used to determine relative CD63 expression levels, normalizing to GAPDH expression as an internal control. B. PBLs cells (1 × 10 5 cells/well) or (C) DCs cells (5 × 10 5 cells/well) were plated in triplicate in 24-well plates and transfected with 50 nM siRNAs (CD63, CD4, and ERBB2IP). Controls included untreated cells or cells treated with virus infection only. Forty eight hours post-transfection, cells were infected with HIV-1 89.6 (m.o.i. = 0.02). Supernatants were harvested for p24 detection on day 5 post-infection for PBLs cells, and on day 7 post-infection for DCs using p24 Capture ELISA kit (ImmunoDiagnostics, Woburn, MA). C. Cell lysates from MDMs on day 7 post-infection were also harvested for detection of intracellular p24. *P

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Tetraspanin CD63 is a regulator of HIV-1 replication

    doi:

    Figure Lengend Snippet: Effects of CD63 silencing on HIV-1 replication in human PBLs and DCs. A. PBLs (1 × 10 5 cells/well) were plated in triplicate in 24-well plates and transfected with 50 nM siRNAs (CD63, CD4, and ERBB2IP). Controls included untreated cells or cells treated with virus infection only. Total mRNA was isolated from each well using the Qiagen RNeasy kit 48 h after transfection. Quantitative RT-PCR was used to determine relative CD63 expression levels, normalizing to GAPDH expression as an internal control. B. PBLs cells (1 × 10 5 cells/well) or (C) DCs cells (5 × 10 5 cells/well) were plated in triplicate in 24-well plates and transfected with 50 nM siRNAs (CD63, CD4, and ERBB2IP). Controls included untreated cells or cells treated with virus infection only. Forty eight hours post-transfection, cells were infected with HIV-1 89.6 (m.o.i. = 0.02). Supernatants were harvested for p24 detection on day 5 post-infection for PBLs cells, and on day 7 post-infection for DCs using p24 Capture ELISA kit (ImmunoDiagnostics, Woburn, MA). C. Cell lysates from MDMs on day 7 post-infection were also harvested for detection of intracellular p24. *P

    Article Snippet: Total mRNA was isolated from siRNA-transfected cells and MDMs using RNeasy Mini Kits (Qiagen).

    Techniques: Transfection, Infection, Isolation, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay

    Electropherogram of RNA elutes obtained using three different RNA extraction methods. a RNA extracted from glucose-supplemented cells using TRI Reagent; b RNA extracted from PE-supplemented cells using TRI Reagent; c RNA extracted from glucose-supplemented cells using RNeasy kit; d RNA extracted from PE-supplemented cells using RNeasy kit; e RNA extracted from glucose-supplemented cells using Phenol-free kit; f RNA extracted from glucose-supplemented cells using Phenol-free kit. NA not available

    Journal: BMC Research Notes

    Article Title: Efficient extraction of small and large RNAs in bacteria for excellent total RNA sequencing and comprehensive transcriptome analysis

    doi: 10.1186/s13104-015-1726-3

    Figure Lengend Snippet: Electropherogram of RNA elutes obtained using three different RNA extraction methods. a RNA extracted from glucose-supplemented cells using TRI Reagent; b RNA extracted from PE-supplemented cells using TRI Reagent; c RNA extracted from glucose-supplemented cells using RNeasy kit; d RNA extracted from PE-supplemented cells using RNeasy kit; e RNA extracted from glucose-supplemented cells using Phenol-free kit; f RNA extracted from glucose-supplemented cells using Phenol-free kit. NA not available

    Article Snippet: With this in mind, we evaluated the performance of Phenol-free total RNA purification kit (Amresco) in comparison with TRI Reagent (MRC) and RNeasy Mini (Qiagen) for the extraction of total RNA of Pseudomonas aeruginosa which was grown in glucose-supplemented (control) and polyethylene-supplemented (growth-limiting condition) minimal medium.

    Techniques: RNA Extraction

    Replication of MHV in mice. Four-week old B6 mice were infected intracranially with 2000 pfu of MHV-A59 and sacrificed at the indicated days post infection. A) Brains were harvested and placed in gelatin saline, homogenized and infectious virus titered on mouse L2 fibroblasts. Titers shown are averages from five mice per group. B) Brains and C) spinal cords were harvested and RNA isolated on Qiagen RNeasy columns. qRT-PCR was performed to quantify relative abundance of MHV-A59 genomic and subgenomic RNA mRNA7. Data are plotted as means with SEM of 5-8 mice.

    Journal: PLoS ONE

    Article Title: Analysis of the Host Transcriptome from Demyelinating Spinal Cord of Murine Coronavirus-Infected Mice

    doi: 10.1371/journal.pone.0075346

    Figure Lengend Snippet: Replication of MHV in mice. Four-week old B6 mice were infected intracranially with 2000 pfu of MHV-A59 and sacrificed at the indicated days post infection. A) Brains were harvested and placed in gelatin saline, homogenized and infectious virus titered on mouse L2 fibroblasts. Titers shown are averages from five mice per group. B) Brains and C) spinal cords were harvested and RNA isolated on Qiagen RNeasy columns. qRT-PCR was performed to quantify relative abundance of MHV-A59 genomic and subgenomic RNA mRNA7. Data are plotted as means with SEM of 5-8 mice.

    Article Snippet: Real time quantitative reverse transcriptase-PCR (qRT-PCR) Total RNA was isolated from homogenized tissue or cultured cells using either RNeasy mini kit columns (Qiagen) or TRIzol (Gibco) according to the manufacturer’s instructions and depleted of DNA using Turbo DNase (Ambion). qRT-PCR was performed as described previously [ ] on each biological replicate.

    Techniques: Mouse Assay, Infection, Isolation, Quantitative RT-PCR

    Morphine Suppresses O 2 -dependent HIF-1α RNA Expression Mouse macrophage RAW-264 cells were pretreated with 1 μM morphine 16 hours prior to administration of 1 μM LPS. Cells were subjected to a hypoxic state (95% N 2 , 5%CO 2 ) for 6 hours. RNA preparations, using the RNeasy Qiagen Mini kit, were reverse transcribed and PCR amplified using HIF-1α primers. Inductions of HIF-1α RNA levels were present under hypoxia (control) and LPS stimuli. Morphine significantly suppressed both hypoxia- and LPS-induced HIF-1α expression.

    Journal: Cellular immunology

    Article Title: Chronic Morphine Treatment Inhibits LPS Induced Angiogenesis: Implications in Wound healing

    doi: 10.1016/j.cellimm.2010.08.002

    Figure Lengend Snippet: Morphine Suppresses O 2 -dependent HIF-1α RNA Expression Mouse macrophage RAW-264 cells were pretreated with 1 μM morphine 16 hours prior to administration of 1 μM LPS. Cells were subjected to a hypoxic state (95% N 2 , 5%CO 2 ) for 6 hours. RNA preparations, using the RNeasy Qiagen Mini kit, were reverse transcribed and PCR amplified using HIF-1α primers. Inductions of HIF-1α RNA levels were present under hypoxia (control) and LPS stimuli. Morphine significantly suppressed both hypoxia- and LPS-induced HIF-1α expression.

    Article Snippet: RNA preparations, using the RNeasy Qiagen Mini kit, were reverse transcribed and PCR amplified using HIF-1α primers.

    Techniques: RNA Expression, Polymerase Chain Reaction, Amplification, Expressing

    Representative gel electrophoresis of PCR reactions using universal bacterial primers and sputum RNA isolated with the RNeasy Plus Mini kit. Sputum cells were either subjected or not to bead vortexing (bashing or glass beads) prior to RNA isolation. Bands representing contaminating bacterial DNA are indicated. M: molecular weight marker.

    Journal: PLoS ONE

    Article Title: Elimination of bacterial DNA during RNA isolation from sputum: Bashing bead vortexing is preferable over prolonged DNase treatment

    doi: 10.1371/journal.pone.0214609

    Figure Lengend Snippet: Representative gel electrophoresis of PCR reactions using universal bacterial primers and sputum RNA isolated with the RNeasy Plus Mini kit. Sputum cells were either subjected or not to bead vortexing (bashing or glass beads) prior to RNA isolation. Bands representing contaminating bacterial DNA are indicated. M: molecular weight marker.

    Article Snippet: Optimizing RNA isolation using the RNeasy Plus Mini kit The RNeasy Plus Mini kit offers two alternative protocols; the standard protocol provides enrichment of the sample with intact mRNAs by eliminating RNAs shorter than 200 nucleotides; the second protocol is designed for the purification of total RNA.

    Techniques: Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Isolation, Molecular Weight, Marker

    Cycle threshold (Ct) values for real-time PCR assays with different base pair (bp) long amplicons of glyceraldehyde 3-phophate dehydrogenase on RNA isolated from sputum samples. Samples were subjected either to repeated digestion process (up to 6 times) with the Turbo DNA-free kit or bead vortexing prior to RNA isolation using the RNeasy Plus Mini kit equipped with the gDNA eliminator spin column (n = 6 for each group). Error bars indicate SEM. # p

    Journal: PLoS ONE

    Article Title: Elimination of bacterial DNA during RNA isolation from sputum: Bashing bead vortexing is preferable over prolonged DNase treatment

    doi: 10.1371/journal.pone.0214609

    Figure Lengend Snippet: Cycle threshold (Ct) values for real-time PCR assays with different base pair (bp) long amplicons of glyceraldehyde 3-phophate dehydrogenase on RNA isolated from sputum samples. Samples were subjected either to repeated digestion process (up to 6 times) with the Turbo DNA-free kit or bead vortexing prior to RNA isolation using the RNeasy Plus Mini kit equipped with the gDNA eliminator spin column (n = 6 for each group). Error bars indicate SEM. # p

    Article Snippet: Optimizing RNA isolation using the RNeasy Plus Mini kit The RNeasy Plus Mini kit offers two alternative protocols; the standard protocol provides enrichment of the sample with intact mRNAs by eliminating RNAs shorter than 200 nucleotides; the second protocol is designed for the purification of total RNA.

    Techniques: Real-time Polymerase Chain Reaction, Isolation