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  • 99
    Qiagen rnease mini kit
    Rnease Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 282 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnease mini kit/product/Qiagen
    Average 99 stars, based on 282 article reviews
    Price from $9.99 to $1999.99
    rnease mini kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Qiagen rneasy mini
    (a) Gel electrophoresis (polyacrylamide 15% stained with ethidium bromide) of LMW RNA samples (LCL) extracted with TRIzol reagent (lane 1), <t>RNEasy</t> kit (lane 2), and small RNA fraction enriched with MirVana kit (lane 3). LMW profile obtained with MirVana kit extraction is similar to that obtained with TRIzol reagent which is not shown for clarity. (b) Agilent 2100 Bioanalyzer electropherogram profile of LMW <t>RNAs</t> (LCL) extracted with TRIzol (black) superimposed on 5.8S (red), 5S (green), and tRNA (blue) bands eluted from polyacrylamide gel. (c) Lymphoblastoid (LCL) LMW RNA profile obtained after plotting the exported raw data from Agilent electropherogram ( ) together with the PeakFit fitted curve (solid line) and the component peak functions. Seven peaks below the LMW RNA profile were fitted by the software ( r 2 = 0.998693).
    Rneasy Mini, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 39424 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy mini/product/Qiagen
    Average 99 stars, based on 39424 article reviews
    Price from $9.99 to $1999.99
    rneasy mini - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    (a) Gel electrophoresis (polyacrylamide 15% stained with ethidium bromide) of LMW RNA samples (LCL) extracted with TRIzol reagent (lane 1), RNEasy kit (lane 2), and small RNA fraction enriched with MirVana kit (lane 3). LMW profile obtained with MirVana kit extraction is similar to that obtained with TRIzol reagent which is not shown for clarity. (b) Agilent 2100 Bioanalyzer electropherogram profile of LMW RNAs (LCL) extracted with TRIzol (black) superimposed on 5.8S (red), 5S (green), and tRNA (blue) bands eluted from polyacrylamide gel. (c) Lymphoblastoid (LCL) LMW RNA profile obtained after plotting the exported raw data from Agilent electropherogram ( ) together with the PeakFit fitted curve (solid line) and the component peak functions. Seven peaks below the LMW RNA profile were fitted by the software ( r 2 = 0.998693).

    Journal: Journal of Biomedicine and Biotechnology

    Article Title: Quantification of Small Non-Coding RNAs Allows an Accurate Comparison of miRNA Expression Profiles

    doi: 10.1155/2009/659028

    Figure Lengend Snippet: (a) Gel electrophoresis (polyacrylamide 15% stained with ethidium bromide) of LMW RNA samples (LCL) extracted with TRIzol reagent (lane 1), RNEasy kit (lane 2), and small RNA fraction enriched with MirVana kit (lane 3). LMW profile obtained with MirVana kit extraction is similar to that obtained with TRIzol reagent which is not shown for clarity. (b) Agilent 2100 Bioanalyzer electropherogram profile of LMW RNAs (LCL) extracted with TRIzol (black) superimposed on 5.8S (red), 5S (green), and tRNA (blue) bands eluted from polyacrylamide gel. (c) Lymphoblastoid (LCL) LMW RNA profile obtained after plotting the exported raw data from Agilent electropherogram ( ) together with the PeakFit fitted curve (solid line) and the component peak functions. Seven peaks below the LMW RNA profile were fitted by the software ( r 2 = 0.998693).

    Article Snippet: Total RNA Extraction and Small RNAs Enrichment Protocols Total RNA was extracted using three different methods: an acid phenol/guanidine isothiocyanate solution (TRIzol Reagent, Invitrogen), a glass-fiber filtration protocol (MirVana miRNA Isolation Kit, Ambion) that provides also a procedure to isolate and enrich low molecular weight (LMW) RNAs from higher molecular weight (HMW) RNAs, and another common glass-fiber purification protocol (RNEasy Mini Kit, Qiagen).

    Techniques: Nucleic Acid Electrophoresis, Staining, Software

    CD63 mRNA down regulation by siRNA in human MDMs which are challenged with different concentration of HIV-1. A. MDMs (5 × 10 5 cells/well) were plated in 24-well plates and transfected with 50 nM siRNAs (CD63, CD4, and ERBB2IP). For controls, cells were treated with AZT (1 mM) or raltegravir (20 mM). Controls also included untreated cells or cells infected with HIV-1 SX (m.o.i. = 0.02) only. Total mRNA was isolated from each well using the Qiagen RNeasy kit 48 h after transfection. Quantitative RT-PCR was used to determine relative CD63 expression levels, normalizing to GAPDH expression as an internal control. B. MDMs were challenged with different concentration of HIV-1 SX (m.o.i. = 0.6, 0.2. 0.06 and 0.02, respectively). Supernatants were harvested for p24 detection on day 7 post-infection for MDMs using p24 Capture ELISA kit (ImmunoDiagnostics, Woburn, MA).

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Tetraspanin CD63 is a regulator of HIV-1 replication

    doi:

    Figure Lengend Snippet: CD63 mRNA down regulation by siRNA in human MDMs which are challenged with different concentration of HIV-1. A. MDMs (5 × 10 5 cells/well) were plated in 24-well plates and transfected with 50 nM siRNAs (CD63, CD4, and ERBB2IP). For controls, cells were treated with AZT (1 mM) or raltegravir (20 mM). Controls also included untreated cells or cells infected with HIV-1 SX (m.o.i. = 0.02) only. Total mRNA was isolated from each well using the Qiagen RNeasy kit 48 h after transfection. Quantitative RT-PCR was used to determine relative CD63 expression levels, normalizing to GAPDH expression as an internal control. B. MDMs were challenged with different concentration of HIV-1 SX (m.o.i. = 0.6, 0.2. 0.06 and 0.02, respectively). Supernatants were harvested for p24 detection on day 7 post-infection for MDMs using p24 Capture ELISA kit (ImmunoDiagnostics, Woburn, MA).

    Article Snippet: Total mRNA was isolated from siRNA-transfected cells and MDMs using RNeasy Mini Kits (Qiagen).

    Techniques: Concentration Assay, Transfection, Infection, Isolation, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay

    Effects of CD63 silencing on HIV-1 replication in human PBLs and DCs. A. PBLs (1 × 10 5 cells/well) were plated in triplicate in 24-well plates and transfected with 50 nM siRNAs (CD63, CD4, and ERBB2IP). Controls included untreated cells or cells treated with virus infection only. Total mRNA was isolated from each well using the Qiagen RNeasy kit 48 h after transfection. Quantitative RT-PCR was used to determine relative CD63 expression levels, normalizing to GAPDH expression as an internal control. B. PBLs cells (1 × 10 5 cells/well) or (C) DCs cells (5 × 10 5 cells/well) were plated in triplicate in 24-well plates and transfected with 50 nM siRNAs (CD63, CD4, and ERBB2IP). Controls included untreated cells or cells treated with virus infection only. Forty eight hours post-transfection, cells were infected with HIV-1 89.6 (m.o.i. = 0.02). Supernatants were harvested for p24 detection on day 5 post-infection for PBLs cells, and on day 7 post-infection for DCs using p24 Capture ELISA kit (ImmunoDiagnostics, Woburn, MA). C. Cell lysates from MDMs on day 7 post-infection were also harvested for detection of intracellular p24. *P

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Tetraspanin CD63 is a regulator of HIV-1 replication

    doi:

    Figure Lengend Snippet: Effects of CD63 silencing on HIV-1 replication in human PBLs and DCs. A. PBLs (1 × 10 5 cells/well) were plated in triplicate in 24-well plates and transfected with 50 nM siRNAs (CD63, CD4, and ERBB2IP). Controls included untreated cells or cells treated with virus infection only. Total mRNA was isolated from each well using the Qiagen RNeasy kit 48 h after transfection. Quantitative RT-PCR was used to determine relative CD63 expression levels, normalizing to GAPDH expression as an internal control. B. PBLs cells (1 × 10 5 cells/well) or (C) DCs cells (5 × 10 5 cells/well) were plated in triplicate in 24-well plates and transfected with 50 nM siRNAs (CD63, CD4, and ERBB2IP). Controls included untreated cells or cells treated with virus infection only. Forty eight hours post-transfection, cells were infected with HIV-1 89.6 (m.o.i. = 0.02). Supernatants were harvested for p24 detection on day 5 post-infection for PBLs cells, and on day 7 post-infection for DCs using p24 Capture ELISA kit (ImmunoDiagnostics, Woburn, MA). C. Cell lysates from MDMs on day 7 post-infection were also harvested for detection of intracellular p24. *P

    Article Snippet: Total mRNA was isolated from siRNA-transfected cells and MDMs using RNeasy Mini Kits (Qiagen).

    Techniques: Transfection, Infection, Isolation, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay

    Replication of MHV in mice. Four-week old B6 mice were infected intracranially with 2000 pfu of MHV-A59 and sacrificed at the indicated days post infection. A) Brains were harvested and placed in gelatin saline, homogenized and infectious virus titered on mouse L2 fibroblasts. Titers shown are averages from five mice per group. B) Brains and C) spinal cords were harvested and RNA isolated on Qiagen RNeasy columns. qRT-PCR was performed to quantify relative abundance of MHV-A59 genomic and subgenomic RNA mRNA7. Data are plotted as means with SEM of 5-8 mice.

    Journal: PLoS ONE

    Article Title: Analysis of the Host Transcriptome from Demyelinating Spinal Cord of Murine Coronavirus-Infected Mice

    doi: 10.1371/journal.pone.0075346

    Figure Lengend Snippet: Replication of MHV in mice. Four-week old B6 mice were infected intracranially with 2000 pfu of MHV-A59 and sacrificed at the indicated days post infection. A) Brains were harvested and placed in gelatin saline, homogenized and infectious virus titered on mouse L2 fibroblasts. Titers shown are averages from five mice per group. B) Brains and C) spinal cords were harvested and RNA isolated on Qiagen RNeasy columns. qRT-PCR was performed to quantify relative abundance of MHV-A59 genomic and subgenomic RNA mRNA7. Data are plotted as means with SEM of 5-8 mice.

    Article Snippet: Real time quantitative reverse transcriptase-PCR (qRT-PCR) Total RNA was isolated from homogenized tissue or cultured cells using either RNeasy mini kit columns (Qiagen) or TRIzol (Gibco) according to the manufacturer’s instructions and depleted of DNA using Turbo DNase (Ambion). qRT-PCR was performed as described previously [ ] on each biological replicate.

    Techniques: Mouse Assay, Infection, Isolation, Quantitative RT-PCR

    Regulation of Eno expression by RNase III and/or RNase G. (a) Effects of rnc and/or rng deletion on the expression level of eno . Escherichia coli MG1655 strains (WT, Δ rng , Δ rnc , and Δ rnc rng ) were grown in LB medium at 37 °C to mid-log phase and harvested for western blot analysis of Eno, Rng, and Rnc using protein-specific polyclonal antibodies. The expression levels of Eno, Rng, and Rnc were compared by setting those of WT to 1. (b) Independent modulation of Eno expression levels by RNase G and RNase III. Western blotting was performed as described for (a) using Δ rnc rng strains harbouring pPM30, pRNG3, or pRNC3. The expression levels of Eno, Rng, and Rnc were compared by setting those of Δ rnc rng harbouring pPM30 to 1. (c) Effects of rnc and/or rng deletion on the eno mRNA abundance. Total cellular RNA was extracted from cultures grown to an OD 600 of 0.6 using an RNeasy mini prep kit. The number of amplicons of enolase and other rnpB mRNA amplified from the cDNAs of the (left) WT, Δ rnc . Δ rng and Δ rnc rng strains (right) harbouring pPM30, pRNC3, or pRNG3. The eno mRNA expression levels were compared by setting those of WT or Δ rnc rng harbouring pPM30 to 1. PCR products were resolved in an 1.5% agarose gel. (d) Identification of the regulatory DNA region that affected the eno expression levels. Top: Schematic diagram of the eno-cat reporter. Bottom: Effects of RNase G and RNase III expression levels on the degree of chloramphenicol resistance of MG1655 cells. MG1655 WT, Δ rng , and Δ rnc cells harbouring pERS1 were transformed with pPM30, pRNG3 (RNase G), or pRNC3 (RNase III). The transformants were grown in LB containing 1 mM IPTG to an OD 600 of 0.6, diluted, and spotted on LB agar containing 0 (Cm 0) or 75 (Cm 75) μg ml −1 chloramphenicol. For (a,b) , the S1 protein was used as an internal standard to evaluate the amount of cell extract in each lane. For (c) , the rnpB mRNA was used as an internal standard to evaluate the amount of cell extract in each lane. For (a–c) , the data are presented as means ± s. e. m. of at least three independent experiments.

    Journal: Scientific Reports

    Article Title: The coordinated action of RNase III and RNase G controls enolase expression in response to oxygen availability in Escherichia coli

    doi: 10.1038/s41598-019-53883-y

    Figure Lengend Snippet: Regulation of Eno expression by RNase III and/or RNase G. (a) Effects of rnc and/or rng deletion on the expression level of eno . Escherichia coli MG1655 strains (WT, Δ rng , Δ rnc , and Δ rnc rng ) were grown in LB medium at 37 °C to mid-log phase and harvested for western blot analysis of Eno, Rng, and Rnc using protein-specific polyclonal antibodies. The expression levels of Eno, Rng, and Rnc were compared by setting those of WT to 1. (b) Independent modulation of Eno expression levels by RNase G and RNase III. Western blotting was performed as described for (a) using Δ rnc rng strains harbouring pPM30, pRNG3, or pRNC3. The expression levels of Eno, Rng, and Rnc were compared by setting those of Δ rnc rng harbouring pPM30 to 1. (c) Effects of rnc and/or rng deletion on the eno mRNA abundance. Total cellular RNA was extracted from cultures grown to an OD 600 of 0.6 using an RNeasy mini prep kit. The number of amplicons of enolase and other rnpB mRNA amplified from the cDNAs of the (left) WT, Δ rnc . Δ rng and Δ rnc rng strains (right) harbouring pPM30, pRNC3, or pRNG3. The eno mRNA expression levels were compared by setting those of WT or Δ rnc rng harbouring pPM30 to 1. PCR products were resolved in an 1.5% agarose gel. (d) Identification of the regulatory DNA region that affected the eno expression levels. Top: Schematic diagram of the eno-cat reporter. Bottom: Effects of RNase G and RNase III expression levels on the degree of chloramphenicol resistance of MG1655 cells. MG1655 WT, Δ rng , and Δ rnc cells harbouring pERS1 were transformed with pPM30, pRNG3 (RNase G), or pRNC3 (RNase III). The transformants were grown in LB containing 1 mM IPTG to an OD 600 of 0.6, diluted, and spotted on LB agar containing 0 (Cm 0) or 75 (Cm 75) μg ml −1 chloramphenicol. For (a,b) , the S1 protein was used as an internal standard to evaluate the amount of cell extract in each lane. For (c) , the rnpB mRNA was used as an internal standard to evaluate the amount of cell extract in each lane. For (a–c) , the data are presented as means ± s. e. m. of at least three independent experiments.

    Article Snippet: Total RNA samples were prepared from the cultures using an RNeasy mini prep kit (Qiagen).

    Techniques: Expressing, Western Blot, Amplification, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Transformation Assay