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  • 90
    Thermo Fisher qiagen rneasy kit
    Qiagen Rneasy Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Avantor qiagen rneasy kit
    Qiagen Rneasy Kit, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiagen rneasy kit/product/Avantor
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    qiagen rneasy kit - by Bioz Stars, 2020-08
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    99
    Qiagen qiagen rneasy kit
    SPP1 expression is up-regulated in glioma initiating cells ( A ) Representative examples of dot plots and histograms of Rhod 123(–) subpopulations from 5 glioma cell lines were acquired by flow cytometry. Glioma cells (1 × 10 7 ) were trypsinized and incubated with 0.1 μg/ml Rhod123 for 20 min in 37°C. After washing the cells were placed in 37°C for 90 min for Rhod123 exclusion in a dark compartment. Cells kept on ice to inhibit exclusion of Rhod123 were used as a positive control for gating in flow cytometry. Fractions of Rhod123(+) and Rhod123(–) cells were sorted using FACS Aria and a left panel shows a gating strategy. ( B ) Analysis of SPP1, OCT3/4 and NANOG gene expression in Rhod 123(–) subpopulations sorted from four human glioma cell lines. Total RNA was isolated from sorted cells using <t>Qiagen</t> <t>RNeasy</t> kit and the levels of SPP1, NANOG and OCT3/4 mRNA were determined by qPCR in Rhod 123(+) and Rhod 123(–) subpopulations; their expression in Rhod 123(+) subpopulations was taken as 1 (a red line). P values were calculated with a t-test and considered significant when *P ≤ 0.05 and * *P ≤ 0.01. ( C ) Quantification of the expression of Spp1, Oct3/4 and Nanog in Rhod 123(–) subpopulations isolated from rat C6 glioma cells versus their levels in Rhod 123(+) subpopulations taken as 1 (a red line); n = 3.
    Qiagen Rneasy Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 22507 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiagen rneasy kit/product/Qiagen
    Average 99 stars, based on 22507 article reviews
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    qiagen rneasy kit - by Bioz Stars, 2020-08
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    Qiagen kits qiagen rneasy mini kit qiagen gmbh hilden germany
    Morphine Suppresses O 2 -dependent HIF-1α RNA Expression Mouse macrophage RAW-264 cells were pretreated with 1 μM morphine 16 hours prior to administration of 1 μM LPS. Cells were subjected to a hypoxic state (95% N 2 , 5%CO 2 ) for 6 hours. RNA preparations, using the <t>RNeasy</t> <t>Qiagen</t> Mini kit, were reverse transcribed and PCR amplified using HIF-1α primers. Inductions of HIF-1α RNA levels were present under hypoxia (control) and LPS stimuli. Morphine significantly suppressed both hypoxia- and LPS-induced HIF-1α expression.
    Kits Qiagen Rneasy Mini Kit Qiagen Gmbh Hilden Germany, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 438 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen qiagen rneasy
    Morphine Suppresses O 2 -dependent HIF-1α RNA Expression Mouse macrophage RAW-264 cells were pretreated with 1 μM morphine 16 hours prior to administration of 1 μM LPS. Cells were subjected to a hypoxic state (95% N 2 , 5%CO 2 ) for 6 hours. RNA preparations, using the <t>RNeasy</t> <t>Qiagen</t> Mini kit, were reverse transcribed and PCR amplified using HIF-1α primers. Inductions of HIF-1α RNA levels were present under hypoxia (control) and LPS stimuli. Morphine significantly suppressed both hypoxia- and LPS-induced HIF-1α expression.
    Qiagen Rneasy, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2303 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiagen rneasy/product/Qiagen
    Average 99 stars, based on 2303 article reviews
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    Operon Biotech qiagen rneasy kit
    Morphine Suppresses O 2 -dependent HIF-1α RNA Expression Mouse macrophage RAW-264 cells were pretreated with 1 μM morphine 16 hours prior to administration of 1 μM LPS. Cells were subjected to a hypoxic state (95% N 2 , 5%CO 2 ) for 6 hours. RNA preparations, using the <t>RNeasy</t> <t>Qiagen</t> Mini kit, were reverse transcribed and PCR amplified using HIF-1α primers. Inductions of HIF-1α RNA levels were present under hypoxia (control) and LPS stimuli. Morphine significantly suppressed both hypoxia- and LPS-induced HIF-1α expression.
    Qiagen Rneasy Kit, supplied by Operon Biotech, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiagen rneasy kit/product/Operon Biotech
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    Qiagen qiagen rneasy midi kit
    Morphine Suppresses O 2 -dependent HIF-1α RNA Expression Mouse macrophage RAW-264 cells were pretreated with 1 μM morphine 16 hours prior to administration of 1 μM LPS. Cells were subjected to a hypoxic state (95% N 2 , 5%CO 2 ) for 6 hours. RNA preparations, using the <t>RNeasy</t> <t>Qiagen</t> Mini kit, were reverse transcribed and PCR amplified using HIF-1α primers. Inductions of HIF-1α RNA levels were present under hypoxia (control) and LPS stimuli. Morphine significantly suppressed both hypoxia- and LPS-induced HIF-1α expression.
    Qiagen Rneasy Midi Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiagen rneasy midi kit/product/Qiagen
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    Qiagen qiagen kits
    Morphine Suppresses O 2 -dependent HIF-1α RNA Expression Mouse macrophage RAW-264 cells were pretreated with 1 μM morphine 16 hours prior to administration of 1 μM LPS. Cells were subjected to a hypoxic state (95% N 2 , 5%CO 2 ) for 6 hours. RNA preparations, using the <t>RNeasy</t> <t>Qiagen</t> Mini kit, were reverse transcribed and PCR amplified using HIF-1α primers. Inductions of HIF-1α RNA levels were present under hypoxia (control) and LPS stimuli. Morphine significantly suppressed both hypoxia- and LPS-induced HIF-1α expression.
    Qiagen Kits, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2384 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 2384 article reviews
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    89
    Qiagen qiagen rneasy mini kits
    Morphine Suppresses O 2 -dependent HIF-1α RNA Expression Mouse macrophage RAW-264 cells were pretreated with 1 μM morphine 16 hours prior to administration of 1 μM LPS. Cells were subjected to a hypoxic state (95% N 2 , 5%CO 2 ) for 6 hours. RNA preparations, using the <t>RNeasy</t> <t>Qiagen</t> Mini kit, were reverse transcribed and PCR amplified using HIF-1α primers. Inductions of HIF-1α RNA levels were present under hypoxia (control) and LPS stimuli. Morphine significantly suppressed both hypoxia- and LPS-induced HIF-1α expression.
    Qiagen Rneasy Mini Kits, supplied by Qiagen, used in various techniques. Bioz Stars score: 89/100, based on 168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiagen rneasy mini kits/product/Qiagen
    Average 89 stars, based on 168 article reviews
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    Qiagen rneasy ffpe kit qiagen
    Morphine Suppresses O 2 -dependent HIF-1α RNA Expression Mouse macrophage RAW-264 cells were pretreated with 1 μM morphine 16 hours prior to administration of 1 μM LPS. Cells were subjected to a hypoxic state (95% N 2 , 5%CO 2 ) for 6 hours. RNA preparations, using the <t>RNeasy</t> <t>Qiagen</t> Mini kit, were reverse transcribed and PCR amplified using HIF-1α primers. Inductions of HIF-1α RNA levels were present under hypoxia (control) and LPS stimuli. Morphine significantly suppressed both hypoxia- and LPS-induced HIF-1α expression.
    Rneasy Ffpe Kit Qiagen, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    SPP1 expression is up-regulated in glioma initiating cells ( A ) Representative examples of dot plots and histograms of Rhod 123(–) subpopulations from 5 glioma cell lines were acquired by flow cytometry. Glioma cells (1 × 10 7 ) were trypsinized and incubated with 0.1 μg/ml Rhod123 for 20 min in 37°C. After washing the cells were placed in 37°C for 90 min for Rhod123 exclusion in a dark compartment. Cells kept on ice to inhibit exclusion of Rhod123 were used as a positive control for gating in flow cytometry. Fractions of Rhod123(+) and Rhod123(–) cells were sorted using FACS Aria and a left panel shows a gating strategy. ( B ) Analysis of SPP1, OCT3/4 and NANOG gene expression in Rhod 123(–) subpopulations sorted from four human glioma cell lines. Total RNA was isolated from sorted cells using Qiagen RNeasy kit and the levels of SPP1, NANOG and OCT3/4 mRNA were determined by qPCR in Rhod 123(+) and Rhod 123(–) subpopulations; their expression in Rhod 123(+) subpopulations was taken as 1 (a red line). P values were calculated with a t-test and considered significant when *P ≤ 0.05 and * *P ≤ 0.01. ( C ) Quantification of the expression of Spp1, Oct3/4 and Nanog in Rhod 123(–) subpopulations isolated from rat C6 glioma cells versus their levels in Rhod 123(+) subpopulations taken as 1 (a red line); n = 3.

    Journal: Oncotarget

    Article Title: The embryonic type of SPP1 transcriptional regulation is re-activated in glioblastoma

    doi: 10.18632/oncotarget.14092

    Figure Lengend Snippet: SPP1 expression is up-regulated in glioma initiating cells ( A ) Representative examples of dot plots and histograms of Rhod 123(–) subpopulations from 5 glioma cell lines were acquired by flow cytometry. Glioma cells (1 × 10 7 ) were trypsinized and incubated with 0.1 μg/ml Rhod123 for 20 min in 37°C. After washing the cells were placed in 37°C for 90 min for Rhod123 exclusion in a dark compartment. Cells kept on ice to inhibit exclusion of Rhod123 were used as a positive control for gating in flow cytometry. Fractions of Rhod123(+) and Rhod123(–) cells were sorted using FACS Aria and a left panel shows a gating strategy. ( B ) Analysis of SPP1, OCT3/4 and NANOG gene expression in Rhod 123(–) subpopulations sorted from four human glioma cell lines. Total RNA was isolated from sorted cells using Qiagen RNeasy kit and the levels of SPP1, NANOG and OCT3/4 mRNA were determined by qPCR in Rhod 123(+) and Rhod 123(–) subpopulations; their expression in Rhod 123(+) subpopulations was taken as 1 (a red line). P values were calculated with a t-test and considered significant when *P ≤ 0.05 and * *P ≤ 0.01. ( C ) Quantification of the expression of Spp1, Oct3/4 and Nanog in Rhod 123(–) subpopulations isolated from rat C6 glioma cells versus their levels in Rhod 123(+) subpopulations taken as 1 (a red line); n = 3.

    Article Snippet: Real- time PCR Total RNA was isolated from glioma cells using Qiagen RNeasy kit, 1 μg was used as a template.

    Techniques: Expressing, Flow Cytometry, Cytometry, Incubation, Positive Control, FACS, Isolation, Real-time Polymerase Chain Reaction

    Morphine Suppresses O 2 -dependent HIF-1α RNA Expression Mouse macrophage RAW-264 cells were pretreated with 1 μM morphine 16 hours prior to administration of 1 μM LPS. Cells were subjected to a hypoxic state (95% N 2 , 5%CO 2 ) for 6 hours. RNA preparations, using the RNeasy Qiagen Mini kit, were reverse transcribed and PCR amplified using HIF-1α primers. Inductions of HIF-1α RNA levels were present under hypoxia (control) and LPS stimuli. Morphine significantly suppressed both hypoxia- and LPS-induced HIF-1α expression.

    Journal: Cellular immunology

    Article Title: Chronic Morphine Treatment Inhibits LPS Induced Angiogenesis: Implications in Wound healing

    doi: 10.1016/j.cellimm.2010.08.002

    Figure Lengend Snippet: Morphine Suppresses O 2 -dependent HIF-1α RNA Expression Mouse macrophage RAW-264 cells were pretreated with 1 μM morphine 16 hours prior to administration of 1 μM LPS. Cells were subjected to a hypoxic state (95% N 2 , 5%CO 2 ) for 6 hours. RNA preparations, using the RNeasy Qiagen Mini kit, were reverse transcribed and PCR amplified using HIF-1α primers. Inductions of HIF-1α RNA levels were present under hypoxia (control) and LPS stimuli. Morphine significantly suppressed both hypoxia- and LPS-induced HIF-1α expression.

    Article Snippet: RNA preparations, using the RNeasy Qiagen Mini kit, were reverse transcribed and PCR amplified using HIF-1α primers.

    Techniques: RNA Expression, Polymerase Chain Reaction, Amplification, Expressing