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  • 99
    Qiagen rnease mini kit
    Quantitative RT-PCR analysis of PAI-1 mRNA levels in patients. The total <t>RNA</t> was harvested from the peripheral blood mononuclear cells using an <t>RNeasy</t> kit according to the manufacturer’s instructions. The RT-PCR experiments were repeated at least 3 times. RNA was reverse transcribed into cDNA using random primers in a Reverse Transcription II system according to the manufacturer’s instructions. The expression of PAI-1 mRNA was quantified by quantitative PCR using an ABI Prism Sequence Detection System. Template-negative and RT-negative conditions were used as controls. Amplification of the endogenous GAPDH cDNA was monitored. The levels (mean value) of PAI-1 transcripts in patients were calculated. RT-PCR, reverse-transcription-PCR, PAI-1, plasminogen activator inhibitor-1.
    Rnease Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 5374 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnease mini kit/product/Qiagen
    Average 99 stars, based on 5374 article reviews
    Price from $9.99 to $1999.99
    rnease mini kit - by Bioz Stars, 2020-08
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    99
    Qiagen rneazy micro kit
    Sorting small cell populations directly into the lysis buffer of the <t>RNA</t> isolation kit improves RNA quality and yield. ( a ) RQN values ( b ) and RNA yield of RNA samples isolated from a range of cell numbers (5000–200,000) when sorting directly into the lysis buffer of the RNA isolation kit or collecting the cells first into a collection medium. RQN values ( c ) and RNA yield ( d ) of RNA samples isolated from a range of cell numbers (5000–200,000) sorted directly into the lysis buffer or sorted into a collection buffer first. But here the volume of the lysis buffer was amended not to exceed its maximum dilution point caused by the sorting procedure. Left panels show samples isolated with the <t>RNeasy</t> plus micro kit, right panels with the RNAqueous micro kit. Average of two biological replicates is shown. Error bars indicate SEM
    Rneazy Micro Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneazy micro kit/product/Qiagen
    Average 99 stars, based on 126 article reviews
    Price from $9.99 to $1999.99
    rneazy micro kit - by Bioz Stars, 2020-08
    99/100 stars
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    99
    Qiagen rnease plus micro kit
    The decreased levels of <t>CCR5</t> expression by CCR5 shRNA (1005) correlates with that of CCR5 mRNA . huCCR5-293T cells were transduced with lentiviral vectors bearing either shRNA 1005 against CCR5 {CCR5shRNA (1005)} or a control shRNA against firefly luciferase (Luc shRNA). To monitor the expression levels of CCR5 on cell surface, the cells were cultured for 4 days and stained with either PE-Cy5 conjugated anti human CCR5 monoclonal antibody or isotype control. CCR5 and EGFP expression were analyzed by flow cytometry. The percentage number in each quadrant is indicated in each panel (A). To measure the levels of CCR5 mRNA, total RNA was isolated using Qiagen <t>RNeasy</t> extraction kit. Quantitative RT-PCR was performed using IQ5 with iScript one step RT-PCR kit using β-actin as an internal control (B).
    Rnease Plus Micro Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnease plus micro kit/product/Qiagen
    Average 99 stars, based on 165 article reviews
    Price from $9.99 to $1999.99
    rnease plus micro kit - by Bioz Stars, 2020-08
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    99
    Qiagen rnease minelute cleanup kit
    The decreased levels of <t>CCR5</t> expression by CCR5 shRNA (1005) correlates with that of CCR5 mRNA . huCCR5-293T cells were transduced with lentiviral vectors bearing either shRNA 1005 against CCR5 {CCR5shRNA (1005)} or a control shRNA against firefly luciferase (Luc shRNA). To monitor the expression levels of CCR5 on cell surface, the cells were cultured for 4 days and stained with either PE-Cy5 conjugated anti human CCR5 monoclonal antibody or isotype control. CCR5 and EGFP expression were analyzed by flow cytometry. The percentage number in each quadrant is indicated in each panel (A). To measure the levels of CCR5 mRNA, total RNA was isolated using Qiagen <t>RNeasy</t> extraction kit. Quantitative RT-PCR was performed using IQ5 with iScript one step RT-PCR kit using β-actin as an internal control (B).
    Rnease Minelute Cleanup Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnease minelute cleanup kit/product/Qiagen
    Average 99 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    rnease minelute cleanup kit - by Bioz Stars, 2020-08
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    99
    Qiagen rnease plant mini kit
    The decreased levels of <t>CCR5</t> expression by CCR5 shRNA (1005) correlates with that of CCR5 mRNA . huCCR5-293T cells were transduced with lentiviral vectors bearing either shRNA 1005 against CCR5 {CCR5shRNA (1005)} or a control shRNA against firefly luciferase (Luc shRNA). To monitor the expression levels of CCR5 on cell surface, the cells were cultured for 4 days and stained with either PE-Cy5 conjugated anti human CCR5 monoclonal antibody or isotype control. CCR5 and EGFP expression were analyzed by flow cytometry. The percentage number in each quadrant is indicated in each panel (A). To measure the levels of CCR5 mRNA, total RNA was isolated using Qiagen <t>RNeasy</t> extraction kit. Quantitative RT-PCR was performed using IQ5 with iScript one step RT-PCR kit using β-actin as an internal control (B).
    Rnease Plant Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnease plant mini kit/product/Qiagen
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    rnease plant mini kit - by Bioz Stars, 2020-08
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    99
    Qiagen rneasy mini kit rneasy mini
    The decreased levels of <t>CCR5</t> expression by CCR5 shRNA (1005) correlates with that of CCR5 mRNA . huCCR5-293T cells were transduced with lentiviral vectors bearing either shRNA 1005 against CCR5 {CCR5shRNA (1005)} or a control shRNA against firefly luciferase (Luc shRNA). To monitor the expression levels of CCR5 on cell surface, the cells were cultured for 4 days and stained with either PE-Cy5 conjugated anti human CCR5 monoclonal antibody or isotype control. CCR5 and EGFP expression were analyzed by flow cytometry. The percentage number in each quadrant is indicated in each panel (A). To measure the levels of CCR5 mRNA, total RNA was isolated using Qiagen <t>RNeasy</t> extraction kit. Quantitative RT-PCR was performed using IQ5 with iScript one step RT-PCR kit using β-actin as an internal control (B).
    Rneasy Mini Kit Rneasy Mini, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy mini kit rneasy mini/product/Qiagen
    Average 99 stars, based on 131 article reviews
    Price from $9.99 to $1999.99
    rneasy mini kit rneasy mini - by Bioz Stars, 2020-08
    99/100 stars
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    99
    Qiagen rneasy ffpe
    The decreased levels of <t>CCR5</t> expression by CCR5 shRNA (1005) correlates with that of CCR5 mRNA . huCCR5-293T cells were transduced with lentiviral vectors bearing either shRNA 1005 against CCR5 {CCR5shRNA (1005)} or a control shRNA against firefly luciferase (Luc shRNA). To monitor the expression levels of CCR5 on cell surface, the cells were cultured for 4 days and stained with either PE-Cy5 conjugated anti human CCR5 monoclonal antibody or isotype control. CCR5 and EGFP expression were analyzed by flow cytometry. The percentage number in each quadrant is indicated in each panel (A). To measure the levels of CCR5 mRNA, total RNA was isolated using Qiagen <t>RNeasy</t> extraction kit. Quantitative RT-PCR was performed using IQ5 with iScript one step RT-PCR kit using β-actin as an internal control (B).
    Rneasy Ffpe, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy ffpe/product/Qiagen
    Average 99 stars, based on 129 article reviews
    Price from $9.99 to $1999.99
    rneasy ffpe - by Bioz Stars, 2020-08
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    99
    Qiagen rneasy 96 kit
    Concentration-dependent reduction of HCV replicon RNA levels by VX-950. HCV replicon cells were incubated with various concentrations of VX-950 for 48 h. At the end of the 48-h incubation, total cellular RNA was extracted by <t>RNeasy-96.</t> The level of HCV replicon RNA remaining was then determined by QRT-PCR, as described in Materials and Methods, and was shown as a percentage of replicon RNA levels in control cells incubated with 0.5% DMSO. Each bar represents the average of results from 5 cell culture replicates with the SD. Data for one representative IC 50 determination is shown.
    Rneasy 96 Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy 96 kit/product/Qiagen
    Average 99 stars, based on 1115 article reviews
    Price from $9.99 to $1999.99
    rneasy 96 kit - by Bioz Stars, 2020-08
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    Quantitative RT-PCR analysis of PAI-1 mRNA levels in patients. The total RNA was harvested from the peripheral blood mononuclear cells using an RNeasy kit according to the manufacturer’s instructions. The RT-PCR experiments were repeated at least 3 times. RNA was reverse transcribed into cDNA using random primers in a Reverse Transcription II system according to the manufacturer’s instructions. The expression of PAI-1 mRNA was quantified by quantitative PCR using an ABI Prism Sequence Detection System. Template-negative and RT-negative conditions were used as controls. Amplification of the endogenous GAPDH cDNA was monitored. The levels (mean value) of PAI-1 transcripts in patients were calculated. RT-PCR, reverse-transcription-PCR, PAI-1, plasminogen activator inhibitor-1.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Therapeutic effects of calcium dobesilate on diabetic nephropathy mediated through reduction of expression of PAI-1

    doi: 10.3892/etm.2012.755

    Figure Lengend Snippet: Quantitative RT-PCR analysis of PAI-1 mRNA levels in patients. The total RNA was harvested from the peripheral blood mononuclear cells using an RNeasy kit according to the manufacturer’s instructions. The RT-PCR experiments were repeated at least 3 times. RNA was reverse transcribed into cDNA using random primers in a Reverse Transcription II system according to the manufacturer’s instructions. The expression of PAI-1 mRNA was quantified by quantitative PCR using an ABI Prism Sequence Detection System. Template-negative and RT-negative conditions were used as controls. Amplification of the endogenous GAPDH cDNA was monitored. The levels (mean value) of PAI-1 transcripts in patients were calculated. RT-PCR, reverse-transcription-PCR, PAI-1, plasminogen activator inhibitor-1.

    Article Snippet: The total RNA was harvested from peripheral blood mononuclear cells using the RNeasy kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions.

    Techniques: Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Expressing, Real-time Polymerase Chain Reaction, Sequencing, Amplification, Polymerase Chain Reaction

    MUC5B-AS1 increases the stability of MUC5B mRNA by forming a protective RNA duplex. a Schematic representation of the PCR amplification regions for overlapping (OL) and non-overlapping (non-OL) regions of MUC5B. We designed two pairs of primers to amplify the OL regions (OL1 and OL2) and non-OL (non-OL1 and non-OL2) regions of MUC5B, respectively. F forward primer, R reverse primer. b RT-PCR products of OL and non-OL regions of MUC5B. Total RNA samples were treated with RNAse A + T cocktail and then cleaned up RNA using RNeasy kits. RT-PCR was conducted using the primers to detect the OL and non-OL regions of the MUC5B mRNA. OL and non-OL regions of KRT7-AS were used as a positive control. c Stability of MUC5B mRNA over 12 h was measured by qRT-PCR relative to time 0 h after blocking new RNA synthesis with Actinomycin D (1 μg/mL; indicated with black arrow). H1299 cells with MUC5B-AS1 or empty vector stable expression were treated with 1 μg/mL ActD, and then harvested cells for RNA purification at 12 h after addition of ActD. Then, MUC5B mRNA stability were subsequently measured by qRT-PCR and were normalized against a synthesized exogenous reference λ polyA + RNA. Student’s t -test, * P

    Journal: Cell Death & Disease

    Article Title: Long non-coding RNA MUC5B-AS1 promotes metastasis through mutually regulating MUC5B expression in lung adenocarcinoma

    doi: 10.1038/s41419-018-0472-6

    Figure Lengend Snippet: MUC5B-AS1 increases the stability of MUC5B mRNA by forming a protective RNA duplex. a Schematic representation of the PCR amplification regions for overlapping (OL) and non-overlapping (non-OL) regions of MUC5B. We designed two pairs of primers to amplify the OL regions (OL1 and OL2) and non-OL (non-OL1 and non-OL2) regions of MUC5B, respectively. F forward primer, R reverse primer. b RT-PCR products of OL and non-OL regions of MUC5B. Total RNA samples were treated with RNAse A + T cocktail and then cleaned up RNA using RNeasy kits. RT-PCR was conducted using the primers to detect the OL and non-OL regions of the MUC5B mRNA. OL and non-OL regions of KRT7-AS were used as a positive control. c Stability of MUC5B mRNA over 12 h was measured by qRT-PCR relative to time 0 h after blocking new RNA synthesis with Actinomycin D (1 μg/mL; indicated with black arrow). H1299 cells with MUC5B-AS1 or empty vector stable expression were treated with 1 μg/mL ActD, and then harvested cells for RNA purification at 12 h after addition of ActD. Then, MUC5B mRNA stability were subsequently measured by qRT-PCR and were normalized against a synthesized exogenous reference λ polyA + RNA. Student’s t -test, * P

    Article Snippet: We then cleaned up RNA using RNeasy kits (QIAGEN, Madison, WI, USA) and carried out reverse transcription-polymerase chain reaction (RT-PCR) using the primers to detect the OL and non-OL regions of the MUC5B mRNA.

    Techniques: Polymerase Chain Reaction, Amplification, Reverse Transcription Polymerase Chain Reaction, Positive Control, Quantitative RT-PCR, Blocking Assay, Plasmid Preparation, Expressing, Purification, Synthesized

    SPP1 expression is up-regulated in glioma initiating cells ( A ) Representative examples of dot plots and histograms of Rhod 123(–) subpopulations from 5 glioma cell lines were acquired by flow cytometry. Glioma cells (1 × 10 7 ) were trypsinized and incubated with 0.1 μg/ml Rhod123 for 20 min in 37°C. After washing the cells were placed in 37°C for 90 min for Rhod123 exclusion in a dark compartment. Cells kept on ice to inhibit exclusion of Rhod123 were used as a positive control for gating in flow cytometry. Fractions of Rhod123(+) and Rhod123(–) cells were sorted using FACS Aria and a left panel shows a gating strategy. ( B ) Analysis of SPP1, OCT3/4 and NANOG gene expression in Rhod 123(–) subpopulations sorted from four human glioma cell lines. Total RNA was isolated from sorted cells using Qiagen RNeasy kit and the levels of SPP1, NANOG and OCT3/4 mRNA were determined by qPCR in Rhod 123(+) and Rhod 123(–) subpopulations; their expression in Rhod 123(+) subpopulations was taken as 1 (a red line). P values were calculated with a t-test and considered significant when *P ≤ 0.05 and * *P ≤ 0.01. ( C ) Quantification of the expression of Spp1, Oct3/4 and Nanog in Rhod 123(–) subpopulations isolated from rat C6 glioma cells versus their levels in Rhod 123(+) subpopulations taken as 1 (a red line); n = 3.

    Journal: Oncotarget

    Article Title: The embryonic type of SPP1 transcriptional regulation is re-activated in glioblastoma

    doi: 10.18632/oncotarget.14092

    Figure Lengend Snippet: SPP1 expression is up-regulated in glioma initiating cells ( A ) Representative examples of dot plots and histograms of Rhod 123(–) subpopulations from 5 glioma cell lines were acquired by flow cytometry. Glioma cells (1 × 10 7 ) were trypsinized and incubated with 0.1 μg/ml Rhod123 for 20 min in 37°C. After washing the cells were placed in 37°C for 90 min for Rhod123 exclusion in a dark compartment. Cells kept on ice to inhibit exclusion of Rhod123 were used as a positive control for gating in flow cytometry. Fractions of Rhod123(+) and Rhod123(–) cells were sorted using FACS Aria and a left panel shows a gating strategy. ( B ) Analysis of SPP1, OCT3/4 and NANOG gene expression in Rhod 123(–) subpopulations sorted from four human glioma cell lines. Total RNA was isolated from sorted cells using Qiagen RNeasy kit and the levels of SPP1, NANOG and OCT3/4 mRNA were determined by qPCR in Rhod 123(+) and Rhod 123(–) subpopulations; their expression in Rhod 123(+) subpopulations was taken as 1 (a red line). P values were calculated with a t-test and considered significant when *P ≤ 0.05 and * *P ≤ 0.01. ( C ) Quantification of the expression of Spp1, Oct3/4 and Nanog in Rhod 123(–) subpopulations isolated from rat C6 glioma cells versus their levels in Rhod 123(+) subpopulations taken as 1 (a red line); n = 3.

    Article Snippet: Real- time PCR Total RNA was isolated from glioma cells using Qiagen RNeasy kit, 1 μg was used as a template.

    Techniques: Expressing, Flow Cytometry, Cytometry, Incubation, Positive Control, FACS, Isolation, Real-time Polymerase Chain Reaction

    Sorting small cell populations directly into the lysis buffer of the RNA isolation kit improves RNA quality and yield. ( a ) RQN values ( b ) and RNA yield of RNA samples isolated from a range of cell numbers (5000–200,000) when sorting directly into the lysis buffer of the RNA isolation kit or collecting the cells first into a collection medium. RQN values ( c ) and RNA yield ( d ) of RNA samples isolated from a range of cell numbers (5000–200,000) sorted directly into the lysis buffer or sorted into a collection buffer first. But here the volume of the lysis buffer was amended not to exceed its maximum dilution point caused by the sorting procedure. Left panels show samples isolated with the RNeasy plus micro kit, right panels with the RNAqueous micro kit. Average of two biological replicates is shown. Error bars indicate SEM

    Journal: BMC Genomics

    Article Title: Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes

    doi: 10.1186/s12864-019-5608-2

    Figure Lengend Snippet: Sorting small cell populations directly into the lysis buffer of the RNA isolation kit improves RNA quality and yield. ( a ) RQN values ( b ) and RNA yield of RNA samples isolated from a range of cell numbers (5000–200,000) when sorting directly into the lysis buffer of the RNA isolation kit or collecting the cells first into a collection medium. RQN values ( c ) and RNA yield ( d ) of RNA samples isolated from a range of cell numbers (5000–200,000) sorted directly into the lysis buffer or sorted into a collection buffer first. But here the volume of the lysis buffer was amended not to exceed its maximum dilution point caused by the sorting procedure. Left panels show samples isolated with the RNeasy plus micro kit, right panels with the RNAqueous micro kit. Average of two biological replicates is shown. Error bars indicate SEM

    Article Snippet: RNA isolation and quality control RNA from sorted cells was isolated with the use of the RNeasy plus micro kit (Qiagen, 74,034) or the RNAqueous-Micro Total RNA Isolation Kit (Ambion, AM1931).

    Techniques: Lysis, Isolation

    Sorting cells directly into the lysis buffer preserves and protects RNA from degradation. RQN values of individual RNA samples isolated at specific time points after FACS sorting of 20,000 fli1a:EGFP cells. Cells were sorted directly in the lysis buffer of the kit or first captured into a collection medium. Left panel shows samples isolated with the RNAqueous micro kit, right panel with the RNeasy plus micro kit

    Journal: BMC Genomics

    Article Title: Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes

    doi: 10.1186/s12864-019-5608-2

    Figure Lengend Snippet: Sorting cells directly into the lysis buffer preserves and protects RNA from degradation. RQN values of individual RNA samples isolated at specific time points after FACS sorting of 20,000 fli1a:EGFP cells. Cells were sorted directly in the lysis buffer of the kit or first captured into a collection medium. Left panel shows samples isolated with the RNAqueous micro kit, right panel with the RNeasy plus micro kit

    Article Snippet: RNA isolation and quality control RNA from sorted cells was isolated with the use of the RNeasy plus micro kit (Qiagen, 74,034) or the RNAqueous-Micro Total RNA Isolation Kit (Ambion, AM1931).

    Techniques: Lysis, Isolation, FACS

    Comparative qualitative analysis of RNA purified with the Rnaqeuous and Rneasy micro kit. ( a ) Boxplots showing RQN values and ( b ) RNA yield of 10 RNA samples purified from 20,000 FACS sorted fli1a:EGFP zebrafish cells with the RNAqueous micro ( left ) and the RNeasy plus micro ( right ) kit. Red dots represent RQN values of individual samples. ( c ) Boxplots showing 5′-3′ delta-Cq (dCq) values calculated from a 5′ and a 3′ RT-qPCR assay of 7 RNA samples isolated from 20,000 FACS sorted fli1a:EGFP zebrafish cells with the RNAqueous micro ( left ) and the RNeasy plus micro ( right ) kit. Red dots represent 5′-3′ dCq values of individual samples. * P

    Journal: BMC Genomics

    Article Title: Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes

    doi: 10.1186/s12864-019-5608-2

    Figure Lengend Snippet: Comparative qualitative analysis of RNA purified with the Rnaqeuous and Rneasy micro kit. ( a ) Boxplots showing RQN values and ( b ) RNA yield of 10 RNA samples purified from 20,000 FACS sorted fli1a:EGFP zebrafish cells with the RNAqueous micro ( left ) and the RNeasy plus micro ( right ) kit. Red dots represent RQN values of individual samples. ( c ) Boxplots showing 5′-3′ delta-Cq (dCq) values calculated from a 5′ and a 3′ RT-qPCR assay of 7 RNA samples isolated from 20,000 FACS sorted fli1a:EGFP zebrafish cells with the RNAqueous micro ( left ) and the RNeasy plus micro ( right ) kit. Red dots represent 5′-3′ dCq values of individual samples. * P

    Article Snippet: RNA isolation and quality control RNA from sorted cells was isolated with the use of the RNeasy plus micro kit (Qiagen, 74,034) or the RNAqueous-Micro Total RNA Isolation Kit (Ambion, AM1931).

    Techniques: Purification, FACS, Quantitative RT-PCR, Isolation

    Comparison of RNA quality using different LCM methods. (A) Graph comparing RNA quality (RIN) from LCM RNA samples captured using the MMI CellCut or Arcturus PixCell Instrument and extracted with either the Arcturus PicoPure Isolation kit or QIAGEN Micro RNeasy kit. An overall significant effect was found for both conditions using a two-way analyses of variance (ANOVA; CellCut vs. PixCell F (1,119) = 114.6; PicoPure vs. QIAGEN F (1,119) = 732.5). Although, it is important to note that two groups (Pixcell PicoPure and CellCut QIAGEN) were solely represented by one tissue type (see Experimental Summary in Table 1 ). There was also a significant interaction between the two conditions (Interaction F (1,119) = 9.177, p = 0.003). (B) The same data shown in A plotted by tissue type. Each tissue (Hippocampus, Midbrain and Liver) showed a significant increase in RIN with the QIAGEN kits vs. PicoPure kits using Sidak’s multiple comparisons post hoc test. All data were normally distributed (passed KS normality test) and had similar variances as tested by Brown-Forsythe test. (C,D) Representative Bioanalyzer gel (top) and electropherogram traces (bottom) from PixCell LCM RNA samples extracted using either the (C) Arcturus PicoPure Isolation kit or (D) QIAGEN Micro RNeasy kit. Note that these LCM samples were acquired simultaneously from different brain regions (CA1 vs. CA2) on the same sections from three mouse brains (#2, #4 or #6). Graphs are plotted min to max with a line at the mean. Numbers in parentheses indicate technical replicates. #### Overall group effect; **** post hoc result p

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Optimized Method for Robust Transcriptome Profiling of Minute Tissues Using Laser Capture Microdissection and Low-Input RNA-Seq

    doi: 10.3389/fnmol.2017.00185

    Figure Lengend Snippet: Comparison of RNA quality using different LCM methods. (A) Graph comparing RNA quality (RIN) from LCM RNA samples captured using the MMI CellCut or Arcturus PixCell Instrument and extracted with either the Arcturus PicoPure Isolation kit or QIAGEN Micro RNeasy kit. An overall significant effect was found for both conditions using a two-way analyses of variance (ANOVA; CellCut vs. PixCell F (1,119) = 114.6; PicoPure vs. QIAGEN F (1,119) = 732.5). Although, it is important to note that two groups (Pixcell PicoPure and CellCut QIAGEN) were solely represented by one tissue type (see Experimental Summary in Table 1 ). There was also a significant interaction between the two conditions (Interaction F (1,119) = 9.177, p = 0.003). (B) The same data shown in A plotted by tissue type. Each tissue (Hippocampus, Midbrain and Liver) showed a significant increase in RIN with the QIAGEN kits vs. PicoPure kits using Sidak’s multiple comparisons post hoc test. All data were normally distributed (passed KS normality test) and had similar variances as tested by Brown-Forsythe test. (C,D) Representative Bioanalyzer gel (top) and electropherogram traces (bottom) from PixCell LCM RNA samples extracted using either the (C) Arcturus PicoPure Isolation kit or (D) QIAGEN Micro RNeasy kit. Note that these LCM samples were acquired simultaneously from different brain regions (CA1 vs. CA2) on the same sections from three mouse brains (#2, #4 or #6). Graphs are plotted min to max with a line at the mean. Numbers in parentheses indicate technical replicates. #### Overall group effect; **** post hoc result p

    Article Snippet: In addition, we also tested whether different methods of RNA isolation had an impact on RNA quality and found that kits with minimal digestion time and temperature, such as 5 min at room temperature in the QIAGEN Micro RNeasy kit, resulted in superior quality RNA (RNA Integrity Number, RIN, 9–10, 10 being intact RNA) compared to longer digestion times at higher temperature, such as 30 min at 42°C in the Arcturus PicoPure RNA Isolation Kit (RIN 6–7), which is the current standard kit used in studies using LCM.

    Techniques: Laser Capture Microdissection, Isolation

    The decreased levels of CCR5 expression by CCR5 shRNA (1005) correlates with that of CCR5 mRNA . huCCR5-293T cells were transduced with lentiviral vectors bearing either shRNA 1005 against CCR5 {CCR5shRNA (1005)} or a control shRNA against firefly luciferase (Luc shRNA). To monitor the expression levels of CCR5 on cell surface, the cells were cultured for 4 days and stained with either PE-Cy5 conjugated anti human CCR5 monoclonal antibody or isotype control. CCR5 and EGFP expression were analyzed by flow cytometry. The percentage number in each quadrant is indicated in each panel (A). To measure the levels of CCR5 mRNA, total RNA was isolated using Qiagen RNeasy extraction kit. Quantitative RT-PCR was performed using IQ5 with iScript one step RT-PCR kit using β-actin as an internal control (B).

    Journal: Genetic Vaccines and Therapy

    Article Title: Characterization of a potent non-cytotoxic shRNA directed to the HIV-1 co-receptor CCR5

    doi: 10.1186/1479-0556-7-8

    Figure Lengend Snippet: The decreased levels of CCR5 expression by CCR5 shRNA (1005) correlates with that of CCR5 mRNA . huCCR5-293T cells were transduced with lentiviral vectors bearing either shRNA 1005 against CCR5 {CCR5shRNA (1005)} or a control shRNA against firefly luciferase (Luc shRNA). To monitor the expression levels of CCR5 on cell surface, the cells were cultured for 4 days and stained with either PE-Cy5 conjugated anti human CCR5 monoclonal antibody or isotype control. CCR5 and EGFP expression were analyzed by flow cytometry. The percentage number in each quadrant is indicated in each panel (A). To measure the levels of CCR5 mRNA, total RNA was isolated using Qiagen RNeasy extraction kit. Quantitative RT-PCR was performed using IQ5 with iScript one step RT-PCR kit using β-actin as an internal control (B).

    Article Snippet: Quantitative RT-PCR for CCR5 mRNA Total RNAs from the infected CEM NKR-CCR5 cells were isolated using the Qiagen RNeasy extraction kit following manufacture's instruction.

    Techniques: Expressing, shRNA, Transduction, Luciferase, Cell Culture, Staining, Flow Cytometry, Cytometry, Isolation, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction

    Concentration-dependent reduction of HCV replicon RNA levels by VX-950. HCV replicon cells were incubated with various concentrations of VX-950 for 48 h. At the end of the 48-h incubation, total cellular RNA was extracted by RNeasy-96. The level of HCV replicon RNA remaining was then determined by QRT-PCR, as described in Materials and Methods, and was shown as a percentage of replicon RNA levels in control cells incubated with 0.5% DMSO. Each bar represents the average of results from 5 cell culture replicates with the SD. Data for one representative IC 50 determination is shown.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Preclinical Profile of VX-950, a Potent, Selective, and Orally Bioavailable Inhibitor of Hepatitis C Virus NS3-4A Serine Protease

    doi: 10.1128/AAC.50.3.899-909.2006

    Figure Lengend Snippet: Concentration-dependent reduction of HCV replicon RNA levels by VX-950. HCV replicon cells were incubated with various concentrations of VX-950 for 48 h. At the end of the 48-h incubation, total cellular RNA was extracted by RNeasy-96. The level of HCV replicon RNA remaining was then determined by QRT-PCR, as described in Materials and Methods, and was shown as a percentage of replicon RNA levels in control cells incubated with 0.5% DMSO. Each bar represents the average of results from 5 cell culture replicates with the SD. Data for one representative IC 50 determination is shown.

    Article Snippet: Total cellular RNA was extracted using an RNeasy-96 kit (QIAGEN, Valencia, CA), and the copy number of the HCV RNA was determined in a quantitative, real-time, multiplex reverse transcription-PCR (QRT-PCR, or Taqman) assay ( ).

    Techniques: Concentration Assay, Incubation, Quantitative RT-PCR, Cell Culture