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  • 99
    Qiagen rnease plus micro kit
    Rnease Plus Micro Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnease plus micro kit/product/Qiagen
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
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    99
    Qiagen rneasy kit
    Gel electrophoresis of total <t>RNA</t> that was obtained from sessile cells with sheared whole-cell lysis coupled to the <t>RNeasy</t> Mini Kit. Lanes 1 , 2 , and 3 represent three independent experiments using the methicillin-resistant S. aureus (MRSA) isolate BMB9393 ( A ) and the methicillin-susceptible S. aureus (MSSA) isolate HC474 ( B ).
    Rneasy Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 98889 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy kit/product/Qiagen
    Average 99 stars, based on 98889 article reviews
    Price from $9.99 to $1999.99
    rneasy kit - by Bioz Stars, 2020-04
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    99
    Qiagen rneasy kits
    Bland-Altman plots for investigating the level of agreement between RNA extraction kits. Each plot shows the differences between the two kits against the averages of the two kits. The lines represent the mean differences and upper and lower limits of agreement (LOA, mean differences ±1.96SD). a Comparison of RNA yield (ng/μl) of samples extracted with High Pure <t>FFPE</t> RNA Micro Kit and <t>RNeasy®</t> FFPE kit. b Comparison of purity (A260/A280) of samples extracted with High Pure FFPE RNA Micro kit and RNeasy® FFPE kit. c Comparison of RIN-values of samples extracted with High Pure FFPE RNA Micro kit and RNeasy® FFPE kit. d Comparison of RNA yield (ng/μl) of samples extracted with RNeasy® FFPE kit and AllPrep® DNA/RNA FFPE kit. e Comparison of purity (A260/A280) of samples extracted with RNeasy® FFPE kit and AllPrep® DNA/RNA FFPE kit. f Comparison of RIN-values of samples extracted with RNeasy® FFPE kit and AllPrep® DNA/RNA FFPE kit
    Rneasy Kits, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 5791 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy kits/product/Qiagen
    Average 99 stars, based on 5791 article reviews
    Price from $9.99 to $1999.99
    rneasy kits - by Bioz Stars, 2020-04
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    99
    Qiagen rnease minelute cleanup kit
    Bland-Altman plots for investigating the level of agreement between RNA extraction kits. Each plot shows the differences between the two kits against the averages of the two kits. The lines represent the mean differences and upper and lower limits of agreement (LOA, mean differences ±1.96SD). a Comparison of RNA yield (ng/μl) of samples extracted with High Pure <t>FFPE</t> RNA Micro Kit and <t>RNeasy®</t> FFPE kit. b Comparison of purity (A260/A280) of samples extracted with High Pure FFPE RNA Micro kit and RNeasy® FFPE kit. c Comparison of RIN-values of samples extracted with High Pure FFPE RNA Micro kit and RNeasy® FFPE kit. d Comparison of RNA yield (ng/μl) of samples extracted with RNeasy® FFPE kit and AllPrep® DNA/RNA FFPE kit. e Comparison of purity (A260/A280) of samples extracted with RNeasy® FFPE kit and AllPrep® DNA/RNA FFPE kit. f Comparison of RIN-values of samples extracted with RNeasy® FFPE kit and AllPrep® DNA/RNA FFPE kit
    Rnease Minelute Cleanup Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnease minelute cleanup kit/product/Qiagen
    Average 99 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
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    99
    Qiagen 96 rneasy kits
    Concentration-dependent reduction of HCV replicon RNA levels by VX-950. HCV replicon cells were incubated with various concentrations of VX-950 for 48 h. At the end of the 48-h incubation, total cellular RNA was extracted by <t>RNeasy-96.</t> The level of HCV replicon RNA remaining was then determined by QRT-PCR, as described in Materials and Methods, and was shown as a percentage of replicon RNA levels in control cells incubated with 0.5% DMSO. Each bar represents the average of results from 5 cell culture replicates with the SD. Data for one representative IC 50 determination is shown.
    96 Rneasy Kits, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/96 rneasy kits/product/Qiagen
    Average 99 stars, based on 52 article reviews
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    Qiagen maxi rneasy kit
    Indel Formation in CD34+ HSPCs Nucleofected with Modified Cas9 <t>mRNAs</t> CD34+ HSPCs were nucleofected with 3 μg of the indicated Cas9 mRNA and 2 μg IL2RGlocus MS-sgRNA. 6 μg Cas9 RNP complexed to 3.2 μg IL2RGlocus MS-sgRNA was nucleofected for comparison. ARCA 5meC/Ψ is our previously published Cas9 mRNA 6 and was also included for comparison. Cells were isolated after 4 days, and indel formation was assessed by TIDE analysis. Bars represent mean ± SEM of at least 5 independent transfections. White and gray bars indicate <t>RNeasy</t> and HPLC-purified mRNAs, respectively. sgRNA complexed to Cas9 RNP was included as a control. ***p
    Maxi Rneasy Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/maxi rneasy kit/product/Qiagen
    Average 99 stars, based on 83 article reviews
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    Qiagen micro rneasy kit
    Comparison of RNA quality using different LCM methods. (A) Graph comparing RNA quality (RIN) from LCM RNA samples captured using the MMI CellCut or Arcturus PixCell Instrument and extracted with either the Arcturus PicoPure Isolation kit or <t>QIAGEN</t> Micro <t>RNeasy</t> kit. An overall significant effect was found for both conditions using a two-way analyses of variance (ANOVA; CellCut vs. PixCell F (1,119) = 114.6; PicoPure vs. QIAGEN F (1,119) = 732.5). Although, it is important to note that two groups (Pixcell PicoPure and CellCut QIAGEN) were solely represented by one tissue type (see Experimental Summary in Table 1 ). There was also a significant interaction between the two conditions (Interaction F (1,119) = 9.177, p = 0.003). (B) The same data shown in A plotted by tissue type. Each tissue (Hippocampus, Midbrain and Liver) showed a significant increase in RIN with the QIAGEN kits vs. PicoPure kits using Sidak’s multiple comparisons post hoc test. All data were normally distributed (passed KS normality test) and had similar variances as tested by Brown-Forsythe test. (C,D) Representative Bioanalyzer gel (top) and electropherogram traces (bottom) from PixCell LCM RNA samples extracted using either the (C) Arcturus PicoPure Isolation kit or (D) QIAGEN Micro RNeasy kit. Note that these LCM samples were acquired simultaneously from different brain regions (CA1 vs. CA2) on the same sections from three mouse brains (#2, #4 or #6). Graphs are plotted min to max with a line at the mean. Numbers in parentheses indicate technical replicates. #### Overall group effect; **** post hoc result p
    Micro Rneasy Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2814 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/micro rneasy kit/product/Qiagen
    Average 99 stars, based on 2814 article reviews
    Price from $9.99 to $1999.99
    micro rneasy kit - by Bioz Stars, 2020-04
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    99
    Qiagen rneasy midi kit
    Image showing nucleotide size distribution from CTAB (panel A) and CTAB + <t>RNeasy</t> <t>RNA</t> isolations (panel B) . Nucleotides have been resolved by denaturing gel electrophoresis and have been stained using ethidium bromide and viewed under a UV transilluminator. In both samples, clear rRNA bands can be seen, suggesting the integrity of the RNAs. In the CTAB gel, a band of high-molecular weight DNA can be seen, this is absent from the CTAB + RNeasy extractions.
    Rneasy Midi Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 3646 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy midi kit/product/Qiagen
    Average 99 stars, based on 3646 article reviews
    Price from $9.99 to $1999.99
    rneasy midi kit - by Bioz Stars, 2020-04
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    Qiagen rnease plus mini kit
    Image showing nucleotide size distribution from CTAB (panel A) and CTAB + <t>RNeasy</t> <t>RNA</t> isolations (panel B) . Nucleotides have been resolved by denaturing gel electrophoresis and have been stained using ethidium bromide and viewed under a UV transilluminator. In both samples, clear rRNA bands can be seen, suggesting the integrity of the RNAs. In the CTAB gel, a band of high-molecular weight DNA can be seen, this is absent from the CTAB + RNeasy extractions.
    Rnease Plus Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnease plus mini kit/product/Qiagen
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    rnease plus mini kit - by Bioz Stars, 2020-04
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    99
    Qiagen rneasy powerbiofilm kit
    Image showing nucleotide size distribution from CTAB (panel A) and CTAB + <t>RNeasy</t> <t>RNA</t> isolations (panel B) . Nucleotides have been resolved by denaturing gel electrophoresis and have been stained using ethidium bromide and viewed under a UV transilluminator. In both samples, clear rRNA bands can be seen, suggesting the integrity of the RNAs. In the CTAB gel, a band of high-molecular weight DNA can be seen, this is absent from the CTAB + RNeasy extractions.
    Rneasy Powerbiofilm Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy powerbiofilm kit/product/Qiagen
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    rneasy powerbiofilm kit - by Bioz Stars, 2020-04
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    Qiagen rneasy powermicrobiome kit
    Image showing nucleotide size distribution from CTAB (panel A) and CTAB + <t>RNeasy</t> <t>RNA</t> isolations (panel B) . Nucleotides have been resolved by denaturing gel electrophoresis and have been stained using ethidium bromide and viewed under a UV transilluminator. In both samples, clear rRNA bands can be seen, suggesting the integrity of the RNAs. In the CTAB gel, a band of high-molecular weight DNA can be seen, this is absent from the CTAB + RNeasy extractions.
    Rneasy Powermicrobiome Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy powermicrobiome kit/product/Qiagen
    Average 99 stars, based on 109 article reviews
    Price from $9.99 to $1999.99
    rneasy powermicrobiome kit - by Bioz Stars, 2020-04
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    Image Search Results


    Gel electrophoresis of total RNA that was obtained from sessile cells with sheared whole-cell lysis coupled to the RNeasy Mini Kit. Lanes 1 , 2 , and 3 represent three independent experiments using the methicillin-resistant S. aureus (MRSA) isolate BMB9393 ( A ) and the methicillin-susceptible S. aureus (MSSA) isolate HC474 ( B ).

    Journal: Brazilian Journal of Medical and Biological Research

    Article Title: Optimization of the RNeasy Mini Kit to obtain high-quality total RNA from sessile cells of Staphylococcus aureus

    doi: 10.1590/1414-431X20154734

    Figure Lengend Snippet: Gel electrophoresis of total RNA that was obtained from sessile cells with sheared whole-cell lysis coupled to the RNeasy Mini Kit. Lanes 1 , 2 , and 3 represent three independent experiments using the methicillin-resistant S. aureus (MRSA) isolate BMB9393 ( A ) and the methicillin-susceptible S. aureus (MSSA) isolate HC474 ( B ).

    Article Snippet: Therefore, we designed a simple optimized protocol using the RNeasy Mini Kit that ensures high-quality RNA preparations from planktonic and sessile cells of MRSA, compatible with experiments that require integrity and good quantity of this molecule.

    Techniques: Nucleic Acid Electrophoresis, Lysis

    CD63 mRNA down regulation by siRNA in human MDMs which are challenged with different concentration of HIV-1. A. MDMs (5 × 10 5 cells/well) were plated in 24-well plates and transfected with 50 nM siRNAs (CD63, CD4, and ERBB2IP). For controls, cells were treated with AZT (1 mM) or raltegravir (20 mM). Controls also included untreated cells or cells infected with HIV-1 SX (m.o.i. = 0.02) only. Total mRNA was isolated from each well using the Qiagen RNeasy kit 48 h after transfection. Quantitative RT-PCR was used to determine relative CD63 expression levels, normalizing to GAPDH expression as an internal control. B. MDMs were challenged with different concentration of HIV-1 SX (m.o.i. = 0.6, 0.2. 0.06 and 0.02, respectively). Supernatants were harvested for p24 detection on day 7 post-infection for MDMs using p24 Capture ELISA kit (ImmunoDiagnostics, Woburn, MA).

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Tetraspanin CD63 is a regulator of HIV-1 replication

    doi:

    Figure Lengend Snippet: CD63 mRNA down regulation by siRNA in human MDMs which are challenged with different concentration of HIV-1. A. MDMs (5 × 10 5 cells/well) were plated in 24-well plates and transfected with 50 nM siRNAs (CD63, CD4, and ERBB2IP). For controls, cells were treated with AZT (1 mM) or raltegravir (20 mM). Controls also included untreated cells or cells infected with HIV-1 SX (m.o.i. = 0.02) only. Total mRNA was isolated from each well using the Qiagen RNeasy kit 48 h after transfection. Quantitative RT-PCR was used to determine relative CD63 expression levels, normalizing to GAPDH expression as an internal control. B. MDMs were challenged with different concentration of HIV-1 SX (m.o.i. = 0.6, 0.2. 0.06 and 0.02, respectively). Supernatants were harvested for p24 detection on day 7 post-infection for MDMs using p24 Capture ELISA kit (ImmunoDiagnostics, Woburn, MA).

    Article Snippet: Total mRNA was isolated from siRNA-transfected cells and MDMs using RNeasy Mini Kits (Qiagen).

    Techniques: Concentration Assay, Transfection, Infection, Isolation, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay

    Effects of CD63 silencing on HIV-1 replication in human PBLs and DCs. A. PBLs (1 × 10 5 cells/well) were plated in triplicate in 24-well plates and transfected with 50 nM siRNAs (CD63, CD4, and ERBB2IP). Controls included untreated cells or cells treated with virus infection only. Total mRNA was isolated from each well using the Qiagen RNeasy kit 48 h after transfection. Quantitative RT-PCR was used to determine relative CD63 expression levels, normalizing to GAPDH expression as an internal control. B. PBLs cells (1 × 10 5 cells/well) or (C) DCs cells (5 × 10 5 cells/well) were plated in triplicate in 24-well plates and transfected with 50 nM siRNAs (CD63, CD4, and ERBB2IP). Controls included untreated cells or cells treated with virus infection only. Forty eight hours post-transfection, cells were infected with HIV-1 89.6 (m.o.i. = 0.02). Supernatants were harvested for p24 detection on day 5 post-infection for PBLs cells, and on day 7 post-infection for DCs using p24 Capture ELISA kit (ImmunoDiagnostics, Woburn, MA). C. Cell lysates from MDMs on day 7 post-infection were also harvested for detection of intracellular p24. *P

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Tetraspanin CD63 is a regulator of HIV-1 replication

    doi:

    Figure Lengend Snippet: Effects of CD63 silencing on HIV-1 replication in human PBLs and DCs. A. PBLs (1 × 10 5 cells/well) were plated in triplicate in 24-well plates and transfected with 50 nM siRNAs (CD63, CD4, and ERBB2IP). Controls included untreated cells or cells treated with virus infection only. Total mRNA was isolated from each well using the Qiagen RNeasy kit 48 h after transfection. Quantitative RT-PCR was used to determine relative CD63 expression levels, normalizing to GAPDH expression as an internal control. B. PBLs cells (1 × 10 5 cells/well) or (C) DCs cells (5 × 10 5 cells/well) were plated in triplicate in 24-well plates and transfected with 50 nM siRNAs (CD63, CD4, and ERBB2IP). Controls included untreated cells or cells treated with virus infection only. Forty eight hours post-transfection, cells were infected with HIV-1 89.6 (m.o.i. = 0.02). Supernatants were harvested for p24 detection on day 5 post-infection for PBLs cells, and on day 7 post-infection for DCs using p24 Capture ELISA kit (ImmunoDiagnostics, Woburn, MA). C. Cell lysates from MDMs on day 7 post-infection were also harvested for detection of intracellular p24. *P

    Article Snippet: Total mRNA was isolated from siRNA-transfected cells and MDMs using RNeasy Mini Kits (Qiagen).

    Techniques: Transfection, Infection, Isolation, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay

    Electropherogram of RNA elutes obtained using three different RNA extraction methods. a RNA extracted from glucose-supplemented cells using TRI Reagent; b RNA extracted from PE-supplemented cells using TRI Reagent; c RNA extracted from glucose-supplemented cells using RNeasy kit; d RNA extracted from PE-supplemented cells using RNeasy kit; e RNA extracted from glucose-supplemented cells using Phenol-free kit; f RNA extracted from glucose-supplemented cells using Phenol-free kit. NA not available

    Journal: BMC Research Notes

    Article Title: Efficient extraction of small and large RNAs in bacteria for excellent total RNA sequencing and comprehensive transcriptome analysis

    doi: 10.1186/s13104-015-1726-3

    Figure Lengend Snippet: Electropherogram of RNA elutes obtained using three different RNA extraction methods. a RNA extracted from glucose-supplemented cells using TRI Reagent; b RNA extracted from PE-supplemented cells using TRI Reagent; c RNA extracted from glucose-supplemented cells using RNeasy kit; d RNA extracted from PE-supplemented cells using RNeasy kit; e RNA extracted from glucose-supplemented cells using Phenol-free kit; f RNA extracted from glucose-supplemented cells using Phenol-free kit. NA not available

    Article Snippet: With this in mind, we evaluated the performance of Phenol-free total RNA purification kit (Amresco) in comparison with TRI Reagent (MRC) and RNeasy Mini (Qiagen) for the extraction of total RNA of Pseudomonas aeruginosa which was grown in glucose-supplemented (control) and polyethylene-supplemented (growth-limiting condition) minimal medium.

    Techniques: RNA Extraction

    Replication of MHV in mice. Four-week old B6 mice were infected intracranially with 2000 pfu of MHV-A59 and sacrificed at the indicated days post infection. A) Brains were harvested and placed in gelatin saline, homogenized and infectious virus titered on mouse L2 fibroblasts. Titers shown are averages from five mice per group. B) Brains and C) spinal cords were harvested and RNA isolated on Qiagen RNeasy columns. qRT-PCR was performed to quantify relative abundance of MHV-A59 genomic and subgenomic RNA mRNA7. Data are plotted as means with SEM of 5-8 mice.

    Journal: PLoS ONE

    Article Title: Analysis of the Host Transcriptome from Demyelinating Spinal Cord of Murine Coronavirus-Infected Mice

    doi: 10.1371/journal.pone.0075346

    Figure Lengend Snippet: Replication of MHV in mice. Four-week old B6 mice were infected intracranially with 2000 pfu of MHV-A59 and sacrificed at the indicated days post infection. A) Brains were harvested and placed in gelatin saline, homogenized and infectious virus titered on mouse L2 fibroblasts. Titers shown are averages from five mice per group. B) Brains and C) spinal cords were harvested and RNA isolated on Qiagen RNeasy columns. qRT-PCR was performed to quantify relative abundance of MHV-A59 genomic and subgenomic RNA mRNA7. Data are plotted as means with SEM of 5-8 mice.

    Article Snippet: Real time quantitative reverse transcriptase-PCR (qRT-PCR) Total RNA was isolated from homogenized tissue or cultured cells using either RNeasy mini kit columns (Qiagen) or TRIzol (Gibco) according to the manufacturer’s instructions and depleted of DNA using Turbo DNase (Ambion). qRT-PCR was performed as described previously [ ] on each biological replicate.

    Techniques: Mouse Assay, Infection, Isolation, Quantitative RT-PCR

    Morphine Suppresses O 2 -dependent HIF-1α RNA Expression Mouse macrophage RAW-264 cells were pretreated with 1 μM morphine 16 hours prior to administration of 1 μM LPS. Cells were subjected to a hypoxic state (95% N 2 , 5%CO 2 ) for 6 hours. RNA preparations, using the RNeasy Qiagen Mini kit, were reverse transcribed and PCR amplified using HIF-1α primers. Inductions of HIF-1α RNA levels were present under hypoxia (control) and LPS stimuli. Morphine significantly suppressed both hypoxia- and LPS-induced HIF-1α expression.

    Journal: Cellular immunology

    Article Title: Chronic Morphine Treatment Inhibits LPS Induced Angiogenesis: Implications in Wound healing

    doi: 10.1016/j.cellimm.2010.08.002

    Figure Lengend Snippet: Morphine Suppresses O 2 -dependent HIF-1α RNA Expression Mouse macrophage RAW-264 cells were pretreated with 1 μM morphine 16 hours prior to administration of 1 μM LPS. Cells were subjected to a hypoxic state (95% N 2 , 5%CO 2 ) for 6 hours. RNA preparations, using the RNeasy Qiagen Mini kit, were reverse transcribed and PCR amplified using HIF-1α primers. Inductions of HIF-1α RNA levels were present under hypoxia (control) and LPS stimuli. Morphine significantly suppressed both hypoxia- and LPS-induced HIF-1α expression.

    Article Snippet: RNA preparations, using the RNeasy Qiagen Mini kit, were reverse transcribed and PCR amplified using HIF-1α primers.

    Techniques: RNA Expression, Polymerase Chain Reaction, Amplification, Expressing

    Bland-Altman plots for investigating the level of agreement between RNA extraction kits. Each plot shows the differences between the two kits against the averages of the two kits. The lines represent the mean differences and upper and lower limits of agreement (LOA, mean differences ±1.96SD). a Comparison of RNA yield (ng/μl) of samples extracted with High Pure FFPE RNA Micro Kit and RNeasy® FFPE kit. b Comparison of purity (A260/A280) of samples extracted with High Pure FFPE RNA Micro kit and RNeasy® FFPE kit. c Comparison of RIN-values of samples extracted with High Pure FFPE RNA Micro kit and RNeasy® FFPE kit. d Comparison of RNA yield (ng/μl) of samples extracted with RNeasy® FFPE kit and AllPrep® DNA/RNA FFPE kit. e Comparison of purity (A260/A280) of samples extracted with RNeasy® FFPE kit and AllPrep® DNA/RNA FFPE kit. f Comparison of RIN-values of samples extracted with RNeasy® FFPE kit and AllPrep® DNA/RNA FFPE kit

    Journal: BMC Medical Research Methodology

    Article Title: Quantity and quality of nucleic acids extracted from archival formalin fixed paraffin embedded prostate biopsies

    doi: 10.1186/s12874-018-0628-1

    Figure Lengend Snippet: Bland-Altman plots for investigating the level of agreement between RNA extraction kits. Each plot shows the differences between the two kits against the averages of the two kits. The lines represent the mean differences and upper and lower limits of agreement (LOA, mean differences ±1.96SD). a Comparison of RNA yield (ng/μl) of samples extracted with High Pure FFPE RNA Micro Kit and RNeasy® FFPE kit. b Comparison of purity (A260/A280) of samples extracted with High Pure FFPE RNA Micro kit and RNeasy® FFPE kit. c Comparison of RIN-values of samples extracted with High Pure FFPE RNA Micro kit and RNeasy® FFPE kit. d Comparison of RNA yield (ng/μl) of samples extracted with RNeasy® FFPE kit and AllPrep® DNA/RNA FFPE kit. e Comparison of purity (A260/A280) of samples extracted with RNeasy® FFPE kit and AllPrep® DNA/RNA FFPE kit. f Comparison of RIN-values of samples extracted with RNeasy® FFPE kit and AllPrep® DNA/RNA FFPE kit

    Article Snippet: The macro-dissected tumor area from one tissue section was used for RNA isolation using the RNeasy® FFPE kit (Qiagen, Hilden, Germany) and the tumor area from the second section was used for DNA isolation using the QIAamp® DNA FFPE Tissue kit (Qiagen, Hilden, Germany), following the manufacturers’ instructions.

    Techniques: RNA Extraction, Formalin-fixed Paraffin-Embedded

    Concentration-dependent reduction of HCV replicon RNA levels by VX-950. HCV replicon cells were incubated with various concentrations of VX-950 for 48 h. At the end of the 48-h incubation, total cellular RNA was extracted by RNeasy-96. The level of HCV replicon RNA remaining was then determined by QRT-PCR, as described in Materials and Methods, and was shown as a percentage of replicon RNA levels in control cells incubated with 0.5% DMSO. Each bar represents the average of results from 5 cell culture replicates with the SD. Data for one representative IC 50 determination is shown.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Preclinical Profile of VX-950, a Potent, Selective, and Orally Bioavailable Inhibitor of Hepatitis C Virus NS3-4A Serine Protease

    doi: 10.1128/AAC.50.3.899-909.2006

    Figure Lengend Snippet: Concentration-dependent reduction of HCV replicon RNA levels by VX-950. HCV replicon cells were incubated with various concentrations of VX-950 for 48 h. At the end of the 48-h incubation, total cellular RNA was extracted by RNeasy-96. The level of HCV replicon RNA remaining was then determined by QRT-PCR, as described in Materials and Methods, and was shown as a percentage of replicon RNA levels in control cells incubated with 0.5% DMSO. Each bar represents the average of results from 5 cell culture replicates with the SD. Data for one representative IC 50 determination is shown.

    Article Snippet: Total cellular RNA was extracted using an RNeasy-96 kit (QIAGEN, Valencia, CA), and the copy number of the HCV RNA was determined in a quantitative, real-time, multiplex reverse transcription-PCR (QRT-PCR, or Taqman) assay ( ).

    Techniques: Concentration Assay, Incubation, Quantitative RT-PCR, Cell Culture

    Indel Formation in CD34+ HSPCs Nucleofected with Modified Cas9 mRNAs CD34+ HSPCs were nucleofected with 3 μg of the indicated Cas9 mRNA and 2 μg IL2RGlocus MS-sgRNA. 6 μg Cas9 RNP complexed to 3.2 μg IL2RGlocus MS-sgRNA was nucleofected for comparison. ARCA 5meC/Ψ is our previously published Cas9 mRNA 6 and was also included for comparison. Cells were isolated after 4 days, and indel formation was assessed by TIDE analysis. Bars represent mean ± SEM of at least 5 independent transfections. White and gray bars indicate RNeasy and HPLC-purified mRNAs, respectively. sgRNA complexed to Cas9 RNP was included as a control. ***p

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification

    doi: 10.1016/j.omtn.2018.06.010

    Figure Lengend Snippet: Indel Formation in CD34+ HSPCs Nucleofected with Modified Cas9 mRNAs CD34+ HSPCs were nucleofected with 3 μg of the indicated Cas9 mRNA and 2 μg IL2RGlocus MS-sgRNA. 6 μg Cas9 RNP complexed to 3.2 μg IL2RGlocus MS-sgRNA was nucleofected for comparison. ARCA 5meC/Ψ is our previously published Cas9 mRNA 6 and was also included for comparison. Cells were isolated after 4 days, and indel formation was assessed by TIDE analysis. Bars represent mean ± SEM of at least 5 independent transfections. White and gray bars indicate RNeasy and HPLC-purified mRNAs, respectively. sgRNA complexed to Cas9 RNP was included as a control. ***p

    Article Snippet: We initially made anti-reverse CleanCap trimers with a 3′-O-methyl group on the sugar of the m7 G to prevent incorporation in the opposite orientation, but we found this to be unnecessary, as the 3′-O-methyl version functioned equivalently to CleanCap with a 3′ OH. mRNAs were purified by RNeasy Maxi (QIAGEN, 75162), phosphatase treated for 1 hr with final 0.25 U/μg Antarctic phosphatase (M0289L) in 1× Antarctic phosphatase buffer, and then re-purified by RNeasy.

    Techniques: Modification, Mass Spectrometry, Isolation, Transfection, High Performance Liquid Chromatography, Purification

    Comparison of RNA quality using different LCM methods. (A) Graph comparing RNA quality (RIN) from LCM RNA samples captured using the MMI CellCut or Arcturus PixCell Instrument and extracted with either the Arcturus PicoPure Isolation kit or QIAGEN Micro RNeasy kit. An overall significant effect was found for both conditions using a two-way analyses of variance (ANOVA; CellCut vs. PixCell F (1,119) = 114.6; PicoPure vs. QIAGEN F (1,119) = 732.5). Although, it is important to note that two groups (Pixcell PicoPure and CellCut QIAGEN) were solely represented by one tissue type (see Experimental Summary in Table 1 ). There was also a significant interaction between the two conditions (Interaction F (1,119) = 9.177, p = 0.003). (B) The same data shown in A plotted by tissue type. Each tissue (Hippocampus, Midbrain and Liver) showed a significant increase in RIN with the QIAGEN kits vs. PicoPure kits using Sidak’s multiple comparisons post hoc test. All data were normally distributed (passed KS normality test) and had similar variances as tested by Brown-Forsythe test. (C,D) Representative Bioanalyzer gel (top) and electropherogram traces (bottom) from PixCell LCM RNA samples extracted using either the (C) Arcturus PicoPure Isolation kit or (D) QIAGEN Micro RNeasy kit. Note that these LCM samples were acquired simultaneously from different brain regions (CA1 vs. CA2) on the same sections from three mouse brains (#2, #4 or #6). Graphs are plotted min to max with a line at the mean. Numbers in parentheses indicate technical replicates. #### Overall group effect; **** post hoc result p

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Optimized Method for Robust Transcriptome Profiling of Minute Tissues Using Laser Capture Microdissection and Low-Input RNA-Seq

    doi: 10.3389/fnmol.2017.00185

    Figure Lengend Snippet: Comparison of RNA quality using different LCM methods. (A) Graph comparing RNA quality (RIN) from LCM RNA samples captured using the MMI CellCut or Arcturus PixCell Instrument and extracted with either the Arcturus PicoPure Isolation kit or QIAGEN Micro RNeasy kit. An overall significant effect was found for both conditions using a two-way analyses of variance (ANOVA; CellCut vs. PixCell F (1,119) = 114.6; PicoPure vs. QIAGEN F (1,119) = 732.5). Although, it is important to note that two groups (Pixcell PicoPure and CellCut QIAGEN) were solely represented by one tissue type (see Experimental Summary in Table 1 ). There was also a significant interaction between the two conditions (Interaction F (1,119) = 9.177, p = 0.003). (B) The same data shown in A plotted by tissue type. Each tissue (Hippocampus, Midbrain and Liver) showed a significant increase in RIN with the QIAGEN kits vs. PicoPure kits using Sidak’s multiple comparisons post hoc test. All data were normally distributed (passed KS normality test) and had similar variances as tested by Brown-Forsythe test. (C,D) Representative Bioanalyzer gel (top) and electropherogram traces (bottom) from PixCell LCM RNA samples extracted using either the (C) Arcturus PicoPure Isolation kit or (D) QIAGEN Micro RNeasy kit. Note that these LCM samples were acquired simultaneously from different brain regions (CA1 vs. CA2) on the same sections from three mouse brains (#2, #4 or #6). Graphs are plotted min to max with a line at the mean. Numbers in parentheses indicate technical replicates. #### Overall group effect; **** post hoc result p

    Article Snippet: In addition, we also tested whether different methods of RNA isolation had an impact on RNA quality and found that kits with minimal digestion time and temperature, such as 5 min at room temperature in the QIAGEN Micro RNeasy kit, resulted in superior quality RNA (RNA Integrity Number, RIN, 9–10, 10 being intact RNA) compared to longer digestion times at higher temperature, such as 30 min at 42°C in the Arcturus PicoPure RNA Isolation Kit (RIN 6–7), which is the current standard kit used in studies using LCM.

    Techniques: Laser Capture Microdissection, Isolation

    Characterization of endogenous pluripotent makers in selected iPS cell lines. Panel A. Total RNA was isolated using RNeasy Micro Kit from selected iPS cell lines (G1–G3, G5, G6), hES H9 cells (H9), and human primary fibroblasts (F). Total RNA (500 ng) was reverse-transcribed using Superscript III Reverse Transcriptase primed with oligo(dT) 12–18 and used as template in subsequent PCR with Taq DNA Polymerase. PCR analysis examined the expression of endogenous Oct4, Nanog, Sox2, as well as ABCG2, Rex1, DNMT3B and hTERT. GAPDH was used as an internal control. N, no template control (N). PCR products were analyzed on a 10% polyacrylamide TBE Precast Gel. Panel B. TRAP assay for telomerase activity. Selected iPS cells (G1–G3, G6), hES H9 cells (H9), and human primary fibroblasts (F) were analyzed for telomerase activity using the TRAPEZE RT Telomerase Detection Kit as described in M M. PCR products were separated on 10% polyacrylamide TBE Precast Gel. Individual samples are as indicated.

    Journal: PLoS ONE

    Article Title: Antibody-Directed Lentiviral Gene Transduction for Live-Cell Monitoring and Selection of Human iPS and hES Cells

    doi: 10.1371/journal.pone.0034778

    Figure Lengend Snippet: Characterization of endogenous pluripotent makers in selected iPS cell lines. Panel A. Total RNA was isolated using RNeasy Micro Kit from selected iPS cell lines (G1–G3, G5, G6), hES H9 cells (H9), and human primary fibroblasts (F). Total RNA (500 ng) was reverse-transcribed using Superscript III Reverse Transcriptase primed with oligo(dT) 12–18 and used as template in subsequent PCR with Taq DNA Polymerase. PCR analysis examined the expression of endogenous Oct4, Nanog, Sox2, as well as ABCG2, Rex1, DNMT3B and hTERT. GAPDH was used as an internal control. N, no template control (N). PCR products were analyzed on a 10% polyacrylamide TBE Precast Gel. Panel B. TRAP assay for telomerase activity. Selected iPS cells (G1–G3, G6), hES H9 cells (H9), and human primary fibroblasts (F) were analyzed for telomerase activity using the TRAPEZE RT Telomerase Detection Kit as described in M M. PCR products were separated on 10% polyacrylamide TBE Precast Gel. Individual samples are as indicated.

    Article Snippet: RT-PCR and PCR Total RNA was harvested using RNeasy Micro Kit (Qiagen) and quantified by spectrophotometer.

    Techniques: Isolation, Polymerase Chain Reaction, Expressing, TRAP Assay, Activity Assay

    Evaluation of total RNA integrity in snap-frozen pancreatic tissues immersed in RNA-later for 24 h at -80ºC that were isolated with the Qiagen kit. Lane 1 shows the status of RNA extracted from rat liver tissue as the control using the same protocol. Lanes 2-4 show the status of RNA extracted after tissues were immersed in RNA-later and immediately placed in liquid nitrogen for 24 h at -80ºC, followed by RNA extraction with Qiagen RNeasy Micro Kits.

    Journal: Iranian Journal of Medical Sciences

    Article Title: Optimization of RNA Extraction from Rat Pancreatic Tissue

    doi:

    Figure Lengend Snippet: Evaluation of total RNA integrity in snap-frozen pancreatic tissues immersed in RNA-later for 24 h at -80ºC that were isolated with the Qiagen kit. Lane 1 shows the status of RNA extracted from rat liver tissue as the control using the same protocol. Lanes 2-4 show the status of RNA extracted after tissues were immersed in RNA-later and immediately placed in liquid nitrogen for 24 h at -80ºC, followed by RNA extraction with Qiagen RNeasy Micro Kits.

    Article Snippet: The tubes that contained pancreatic tissue and RNA-later were processed for extraction by using the RNX-plus solution, TriPure, and RNeasy Micro Kits (Qiagen, USA) according to the manufacturers’ instructions after either 30 min, overnight in 4ºC, or following storage at -80ºC for one, three or seven days in order to compare the effect of preservation time on RNA integrity.

    Techniques: Isolation, RNA Extraction

    Image showing nucleotide size distribution from CTAB (panel A) and CTAB + RNeasy RNA isolations (panel B) . Nucleotides have been resolved by denaturing gel electrophoresis and have been stained using ethidium bromide and viewed under a UV transilluminator. In both samples, clear rRNA bands can be seen, suggesting the integrity of the RNAs. In the CTAB gel, a band of high-molecular weight DNA can be seen, this is absent from the CTAB + RNeasy extractions.

    Journal: BMC Research Notes

    Article Title: Optimization and comparison of different methods for RNA isolation for cDNA library construction from the reindeer lichen Cladonia rangiferina

    doi: 10.1186/1756-0500-2-204

    Figure Lengend Snippet: Image showing nucleotide size distribution from CTAB (panel A) and CTAB + RNeasy RNA isolations (panel B) . Nucleotides have been resolved by denaturing gel electrophoresis and have been stained using ethidium bromide and viewed under a UV transilluminator. In both samples, clear rRNA bands can be seen, suggesting the integrity of the RNAs. In the CTAB gel, a band of high-molecular weight DNA can be seen, this is absent from the CTAB + RNeasy extractions.

    Article Snippet: The extracted total RNA was cleaned using the RNeasy Midi Kit (Qiagen, Germany) according to the manufacturer's instructions.

    Techniques: Nucleic Acid Electrophoresis, Staining, Molecular Weight