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  • 99
    New England Biolabs rnaseh
    H1-TKO cells display impaired transcription dynamics. a Diagram of the experimental design to measure transcription elongation rates by transient inhibition of initiating RNAPIIs with DRB. Three hours after DRB incubation, the drug was washed-off to resume transcription elongation and total RNA was extracted from identical number of cells at the indicated time-points (open triangles). Global nascent transcription was evaluated by 1h-EU labelling at the indicated time points (red lines). b Time course transcription elongation measurements at the Med13l and Inpp5a genes in WT mES (upper panels) and H1-TKO mES cells (lower panels). Levels of pre-mRNA at the indicated times were determined by RT-qPCR at the positions marked in the gene maps above the graphs. Pre-mRNA values were normalized to the values of the non-DRB-treated sample. Results are shown as means ± s.d. from two independent experiments ( n = 2). c Representative images of EU staining (top) and distribution of EU nuclear intensity during DRB treatment and upon drug-release at the time points shown in the experimental scheme in a . d Representative images of S9.6 immunostaining <t>±RNAseA</t> or <t>+-RNAseH</t> incubation (top) and distribution of S9.6 nuclear intensity (bottom) in WT and H1-TKO cells. Scale bar, 10 μm. Nuclear segmentation (white lines) was based on DAPI staining. Median values are indicated ( n c-d for S-phase distribution of S9.6 and γH2AX nuclear intensities. Differences between distributions were assessed with the Mann–Whitney rank sum test. **** P
    Rnaseh, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1140 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript ii rnaseh reverse transcriptase
    H1-TKO cells display impaired transcription dynamics. a Diagram of the experimental design to measure transcription elongation rates by transient inhibition of initiating RNAPIIs with DRB. Three hours after DRB incubation, the drug was washed-off to resume transcription elongation and total RNA was extracted from identical number of cells at the indicated time-points (open triangles). Global nascent transcription was evaluated by 1h-EU labelling at the indicated time points (red lines). b Time course transcription elongation measurements at the Med13l and Inpp5a genes in WT mES (upper panels) and H1-TKO mES cells (lower panels). Levels of pre-mRNA at the indicated times were determined by RT-qPCR at the positions marked in the gene maps above the graphs. Pre-mRNA values were normalized to the values of the non-DRB-treated sample. Results are shown as means ± s.d. from two independent experiments ( n = 2). c Representative images of EU staining (top) and distribution of EU nuclear intensity during DRB treatment and upon drug-release at the time points shown in the experimental scheme in a . d Representative images of S9.6 immunostaining <t>±RNAseA</t> or <t>+-RNAseH</t> incubation (top) and distribution of S9.6 nuclear intensity (bottom) in WT and H1-TKO cells. Scale bar, 10 μm. Nuclear segmentation (white lines) was based on DAPI staining. Median values are indicated ( n c-d for S-phase distribution of S9.6 and γH2AX nuclear intensities. Differences between distributions were assessed with the Mann–Whitney rank sum test. **** P
    Superscript Ii Rnaseh Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 767 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher rnaseh
    Depletion of Tb RAP1 led to increased amount of telomeric <t>RNA:DNA</t> hybrids, which was suppressed by ectopic expression of Tb RNaseH1. ( A ) Dot blot Southern analysis of input (diluted 20-fold), IgG and S9.6 immunoprecipitated DNA samples in S/RAP1i cells (top) and S/RAP1i+RNaseH1-2HA cells (bottom) before (–Dox) and after (+Dox) adding doxycycline for 24 h. Samples were treated with or without <t>RNaseH</t> (Thermo Fisher Scientific) before IP. A (TTAGGG) n probe was used in the hybridization. ( B ) Quantification of the dot blot hybridization signals. Average values were calculated from at least four independent experiments. Standard deviations are shown as error bars. Unpaired t -test P values are indicated.
    Rnaseh, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1962 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii rnaseh reverse transcriptase
    Depletion of Tb RAP1 led to increased amount of telomeric <t>RNA:DNA</t> hybrids, which was suppressed by ectopic expression of Tb RNaseH1. ( A ) Dot blot Southern analysis of input (diluted 20-fold), IgG and S9.6 immunoprecipitated DNA samples in S/RAP1i cells (top) and S/RAP1i+RNaseH1-2HA cells (bottom) before (–Dox) and after (+Dox) adding doxycycline for 24 h. Samples were treated with or without <t>RNaseH</t> (Thermo Fisher Scientific) before IP. A (TTAGGG) n probe was used in the hybridization. ( B ) Quantification of the dot blot hybridization signals. Average values were calculated from at least four independent experiments. Standard deviations are shown as error bars. Unpaired t -test P values are indicated.
    Superscript Iii Rnaseh Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 336 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript rnaseh reverse transcriptase
    Depletion of Tb RAP1 led to increased amount of telomeric <t>RNA:DNA</t> hybrids, which was suppressed by ectopic expression of Tb RNaseH1. ( A ) Dot blot Southern analysis of input (diluted 20-fold), IgG and S9.6 immunoprecipitated DNA samples in S/RAP1i cells (top) and S/RAP1i+RNaseH1-2HA cells (bottom) before (–Dox) and after (+Dox) adding doxycycline for 24 h. Samples were treated with or without <t>RNaseH</t> (Thermo Fisher Scientific) before IP. A (TTAGGG) n probe was used in the hybridization. ( B ) Quantification of the dot blot hybridization signals. Average values were calculated from at least four independent experiments. Standard deviations are shown as error bars. Unpaired t -test P values are indicated.
    Superscript Rnaseh Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Illumina Inc rnaseh
    Depletion of Tb RAP1 led to increased amount of telomeric <t>RNA:DNA</t> hybrids, which was suppressed by ectopic expression of Tb RNaseH1. ( A ) Dot blot Southern analysis of input (diluted 20-fold), IgG and S9.6 immunoprecipitated DNA samples in S/RAP1i cells (top) and S/RAP1i+RNaseH1-2HA cells (bottom) before (–Dox) and after (+Dox) adding doxycycline for 24 h. Samples were treated with or without <t>RNaseH</t> (Thermo Fisher Scientific) before IP. A (TTAGGG) n probe was used in the hybridization. ( B ) Quantification of the dot blot hybridization signals. Average values were calculated from at least four independent experiments. Standard deviations are shown as error bars. Unpaired t -test P values are indicated.
    Rnaseh, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 198 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Promega rnaseh
    Depletion of Tb RAP1 led to increased amount of telomeric <t>RNA:DNA</t> hybrids, which was suppressed by ectopic expression of Tb RNaseH1. ( A ) Dot blot Southern analysis of input (diluted 20-fold), IgG and S9.6 immunoprecipitated DNA samples in S/RAP1i cells (top) and S/RAP1i+RNaseH1-2HA cells (bottom) before (–Dox) and after (+Dox) adding doxycycline for 24 h. Samples were treated with or without <t>RNaseH</t> (Thermo Fisher Scientific) before IP. A (TTAGGG) n probe was used in the hybridization. ( B ) Quantification of the dot blot hybridization signals. Average values were calculated from at least four independent experiments. Standard deviations are shown as error bars. Unpaired t -test P values are indicated.
    Rnaseh, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher superscript ii rnaseh
    Depletion of Tb RAP1 led to increased amount of telomeric <t>RNA:DNA</t> hybrids, which was suppressed by ectopic expression of Tb RNaseH1. ( A ) Dot blot Southern analysis of input (diluted 20-fold), IgG and S9.6 immunoprecipitated DNA samples in S/RAP1i cells (top) and S/RAP1i+RNaseH1-2HA cells (bottom) before (–Dox) and after (+Dox) adding doxycycline for 24 h. Samples were treated with or without <t>RNaseH</t> (Thermo Fisher Scientific) before IP. A (TTAGGG) n probe was used in the hybridization. ( B ) Quantification of the dot blot hybridization signals. Average values were calculated from at least four independent experiments. Standard deviations are shown as error bars. Unpaired t -test P values are indicated.
    Superscript Ii Rnaseh, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Enzymatics rnaseh
    Depletion of Tb RAP1 led to increased amount of telomeric <t>RNA:DNA</t> hybrids, which was suppressed by ectopic expression of Tb RNaseH1. ( A ) Dot blot Southern analysis of input (diluted 20-fold), IgG and S9.6 immunoprecipitated DNA samples in S/RAP1i cells (top) and S/RAP1i+RNaseH1-2HA cells (bottom) before (–Dox) and after (+Dox) adding doxycycline for 24 h. Samples were treated with or without <t>RNaseH</t> (Thermo Fisher Scientific) before IP. A (TTAGGG) n probe was used in the hybridization. ( B ) Quantification of the dot blot hybridization signals. Average values were calculated from at least four independent experiments. Standard deviations are shown as error bars. Unpaired t -test P values are indicated.
    Rnaseh, supplied by Enzymatics, used in various techniques. Bioz Stars score: 92/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher superscript ii rnaseh reverse transcriptase kit
    Depletion of Tb RAP1 led to increased amount of telomeric <t>RNA:DNA</t> hybrids, which was suppressed by ectopic expression of Tb RNaseH1. ( A ) Dot blot Southern analysis of input (diluted 20-fold), IgG and S9.6 immunoprecipitated DNA samples in S/RAP1i cells (top) and S/RAP1i+RNaseH1-2HA cells (bottom) before (–Dox) and after (+Dox) adding doxycycline for 24 h. Samples were treated with or without <t>RNaseH</t> (Thermo Fisher Scientific) before IP. A (TTAGGG) n probe was used in the hybridization. ( B ) Quantification of the dot blot hybridization signals. Average values were calculated from at least four independent experiments. Standard deviations are shown as error bars. Unpaired t -test P values are indicated.
    Superscript Ii Rnaseh Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa sybr premix ex taq tli rnaseh plus
    Depletion of Tb RAP1 led to increased amount of telomeric <t>RNA:DNA</t> hybrids, which was suppressed by ectopic expression of Tb RNaseH1. ( A ) Dot blot Southern analysis of input (diluted 20-fold), IgG and S9.6 immunoprecipitated DNA samples in S/RAP1i cells (top) and S/RAP1i+RNaseH1-2HA cells (bottom) before (–Dox) and after (+Dox) adding doxycycline for 24 h. Samples were treated with or without <t>RNaseH</t> (Thermo Fisher Scientific) before IP. A (TTAGGG) n probe was used in the hybridization. ( B ) Quantification of the dot blot hybridization signals. Average values were calculated from at least four independent experiments. Standard deviations are shown as error bars. Unpaired t -test P values are indicated.
    Sybr Premix Ex Taq Tli Rnaseh Plus, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Toyobo m mlv reverse transcriptase rnaseh
    Depletion of Tb RAP1 led to increased amount of telomeric <t>RNA:DNA</t> hybrids, which was suppressed by ectopic expression of Tb RNaseH1. ( A ) Dot blot Southern analysis of input (diluted 20-fold), IgG and S9.6 immunoprecipitated DNA samples in S/RAP1i cells (top) and S/RAP1i+RNaseH1-2HA cells (bottom) before (–Dox) and after (+Dox) adding doxycycline for 24 h. Samples were treated with or without <t>RNaseH</t> (Thermo Fisher Scientific) before IP. A (TTAGGG) n probe was used in the hybridization. ( B ) Quantification of the dot blot hybridization signals. Average values were calculated from at least four independent experiments. Standard deviations are shown as error bars. Unpaired t -test P values are indicated.
    M Mlv Reverse Transcriptase Rnaseh, supplied by Toyobo, used in various techniques. Bioz Stars score: 88/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher rnaseh treatment in vitro
    DRIP-qPCR signal is eliminated by <t>RNaseH</t> treatment in vitro. ( A ) Diagram of qPCR primer locations in the RPL13A gene. DRIP-qPCR was performed in wild-type and CtIP-depleted cells as in Figure 6 . Half of the sample was treated with RNaseH in vitro before the addition of the <t>S9.6</t> antibody, ‘+RNaseH’ as indicated. n = 3, error bars represent standard deviation. * denotes p
    Rnaseh Treatment In Vitro, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa sybr premix ex taq ii tli rnaseh plus
    DRIP-qPCR signal is eliminated by <t>RNaseH</t> treatment in vitro. ( A ) Diagram of qPCR primer locations in the RPL13A gene. DRIP-qPCR was performed in wild-type and CtIP-depleted cells as in Figure 6 . Half of the sample was treated with RNaseH in vitro before the addition of the <t>S9.6</t> antibody, ‘+RNaseH’ as indicated. n = 3, error bars represent standard deviation. * denotes p
    Sybr Premix Ex Taq Ii Tli Rnaseh Plus, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher ribonuclease h
    DRIP-qPCR signal is eliminated by <t>RNaseH</t> treatment in vitro. ( A ) Diagram of qPCR primer locations in the RPL13A gene. DRIP-qPCR was performed in wild-type and CtIP-depleted cells as in Figure 6 . Half of the sample was treated with RNaseH in vitro before the addition of the <t>S9.6</t> antibody, ‘+RNaseH’ as indicated. n = 3, error bars represent standard deviation. * denotes p
    Ribonuclease H, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 648 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Merck & Co hiv 1 rnaseh
    p66/p51 <t>HIV-1</t> reverse transcriptase containing an RNA/DNA hybrid. (A) Fingers, palm, thumb and connection subdomains are color coded blue, red, green and yellow, respectively, with the darker and lighter colors representing the p66 and p51 subunits, respectively. The p66 C-terminal <t>RNaseH</t> domain is depicted in gold. RNA and DNA strands of the hybrid are depicted as magenta and sand-colored spheres, respectively. (B) Close-up of the p66 RNaseH domain containing portions of the RNA/DNA hybrid described by Lapkouski et al. ]. Structural elements have been outlined, and catalytic residues (cyan) are: D 1 : Asp498; D 2 : Asp549; D 3 : Asp443; E: Glu478. RNA and DNA strands of the RNA/DNA hybrid are depicted in red and blue, respectively. RNaseH: Ribonuclease H.
    Hiv 1 Rnaseh, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher rnaseh reverse transcriptase
    p66/p51 <t>HIV-1</t> reverse transcriptase containing an RNA/DNA hybrid. (A) Fingers, palm, thumb and connection subdomains are color coded blue, red, green and yellow, respectively, with the darker and lighter colors representing the p66 and p51 subunits, respectively. The p66 C-terminal <t>RNaseH</t> domain is depicted in gold. RNA and DNA strands of the hybrid are depicted as magenta and sand-colored spheres, respectively. (B) Close-up of the p66 RNaseH domain containing portions of the RNA/DNA hybrid described by Lapkouski et al. ]. Structural elements have been outlined, and catalytic residues (cyan) are: D 1 : Asp498; D 2 : Asp549; D 3 : Asp443; E: Glu478. RNA and DNA strands of the RNA/DNA hybrid are depicted in red and blue, respectively. RNaseH: Ribonuclease H.
    Rnaseh Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    H1-TKO cells display impaired transcription dynamics. a Diagram of the experimental design to measure transcription elongation rates by transient inhibition of initiating RNAPIIs with DRB. Three hours after DRB incubation, the drug was washed-off to resume transcription elongation and total RNA was extracted from identical number of cells at the indicated time-points (open triangles). Global nascent transcription was evaluated by 1h-EU labelling at the indicated time points (red lines). b Time course transcription elongation measurements at the Med13l and Inpp5a genes in WT mES (upper panels) and H1-TKO mES cells (lower panels). Levels of pre-mRNA at the indicated times were determined by RT-qPCR at the positions marked in the gene maps above the graphs. Pre-mRNA values were normalized to the values of the non-DRB-treated sample. Results are shown as means ± s.d. from two independent experiments ( n = 2). c Representative images of EU staining (top) and distribution of EU nuclear intensity during DRB treatment and upon drug-release at the time points shown in the experimental scheme in a . d Representative images of S9.6 immunostaining ±RNAseA or +-RNAseH incubation (top) and distribution of S9.6 nuclear intensity (bottom) in WT and H1-TKO cells. Scale bar, 10 μm. Nuclear segmentation (white lines) was based on DAPI staining. Median values are indicated ( n c-d for S-phase distribution of S9.6 and γH2AX nuclear intensities. Differences between distributions were assessed with the Mann–Whitney rank sum test. **** P

    Journal: Nature Communications

    Article Title: Chromatin conformation regulates the coordination between DNA replication and transcription

    doi: 10.1038/s41467-018-03539-8

    Figure Lengend Snippet: H1-TKO cells display impaired transcription dynamics. a Diagram of the experimental design to measure transcription elongation rates by transient inhibition of initiating RNAPIIs with DRB. Three hours after DRB incubation, the drug was washed-off to resume transcription elongation and total RNA was extracted from identical number of cells at the indicated time-points (open triangles). Global nascent transcription was evaluated by 1h-EU labelling at the indicated time points (red lines). b Time course transcription elongation measurements at the Med13l and Inpp5a genes in WT mES (upper panels) and H1-TKO mES cells (lower panels). Levels of pre-mRNA at the indicated times were determined by RT-qPCR at the positions marked in the gene maps above the graphs. Pre-mRNA values were normalized to the values of the non-DRB-treated sample. Results are shown as means ± s.d. from two independent experiments ( n = 2). c Representative images of EU staining (top) and distribution of EU nuclear intensity during DRB treatment and upon drug-release at the time points shown in the experimental scheme in a . d Representative images of S9.6 immunostaining ±RNAseA or +-RNAseH incubation (top) and distribution of S9.6 nuclear intensity (bottom) in WT and H1-TKO cells. Scale bar, 10 μm. Nuclear segmentation (white lines) was based on DAPI staining. Median values are indicated ( n c-d for S-phase distribution of S9.6 and γH2AX nuclear intensities. Differences between distributions were assessed with the Mann–Whitney rank sum test. **** P

    Article Snippet: Samples were treated either with 1 mg/ml RNAseA (Sigma), 30 U RNAseH (New England Biolabs), or both RNAseA and RNAseH, for 18 h at 37 °C before immunoprecipitation.

    Techniques: Inhibition, Incubation, Quantitative RT-PCR, Staining, Immunostaining, MANN-WHITNEY

    Depletion of Tb RAP1 led to increased amount of telomeric RNA:DNA hybrids, which was suppressed by ectopic expression of Tb RNaseH1. ( A ) Dot blot Southern analysis of input (diluted 20-fold), IgG and S9.6 immunoprecipitated DNA samples in S/RAP1i cells (top) and S/RAP1i+RNaseH1-2HA cells (bottom) before (–Dox) and after (+Dox) adding doxycycline for 24 h. Samples were treated with or without RNaseH (Thermo Fisher Scientific) before IP. A (TTAGGG) n probe was used in the hybridization. ( B ) Quantification of the dot blot hybridization signals. Average values were calculated from at least four independent experiments. Standard deviations are shown as error bars. Unpaired t -test P values are indicated.

    Journal: Nucleic Acids Research

    Article Title: Trypanosoma brucei RAP1 maintains telomere and subtelomere integrity by suppressing TERRA and telomeric RNA:DNA hybrids

    doi: 10.1093/nar/gkx184

    Figure Lengend Snippet: Depletion of Tb RAP1 led to increased amount of telomeric RNA:DNA hybrids, which was suppressed by ectopic expression of Tb RNaseH1. ( A ) Dot blot Southern analysis of input (diluted 20-fold), IgG and S9.6 immunoprecipitated DNA samples in S/RAP1i cells (top) and S/RAP1i+RNaseH1-2HA cells (bottom) before (–Dox) and after (+Dox) adding doxycycline for 24 h. Samples were treated with or without RNaseH (Thermo Fisher Scientific) before IP. A (TTAGGG) n probe was used in the hybridization. ( B ) Quantification of the dot blot hybridization signals. Average values were calculated from at least four independent experiments. Standard deviations are shown as error bars. Unpaired t -test P values are indicated.

    Article Snippet: Treating genomic DNA with RNaseH (Thermo Fisher Scientific) before S9.6 IP reduced the precipitated telomeric signals to the background level, confirming that the detected signals are from an RNA:DNA hybrid structure.

    Techniques: Expressing, Dot Blot, Immunoprecipitation, Hybridization

    DRIP-qPCR signal is eliminated by RNaseH treatment in vitro. ( A ) Diagram of qPCR primer locations in the RPL13A gene. DRIP-qPCR was performed in wild-type and CtIP-depleted cells as in Figure 6 . Half of the sample was treated with RNaseH in vitro before the addition of the S9.6 antibody, ‘+RNaseH’ as indicated. n = 3, error bars represent standard deviation. * denotes p

    Journal: eLife

    Article Title: Sae2/CtIP prevents R-loop accumulation in eukaryotic cells

    doi: 10.7554/eLife.42733

    Figure Lengend Snippet: DRIP-qPCR signal is eliminated by RNaseH treatment in vitro. ( A ) Diagram of qPCR primer locations in the RPL13A gene. DRIP-qPCR was performed in wild-type and CtIP-depleted cells as in Figure 6 . Half of the sample was treated with RNaseH in vitro before the addition of the S9.6 antibody, ‘+RNaseH’ as indicated. n = 3, error bars represent standard deviation. * denotes p

    Article Snippet: Several experiments require additional control; all studies with S9.6 should provide the control with RNaseH treatment in vitro to show that the signal is removed (S9.6 also recognizes dsRNA and data must assure that the signal detected corresponds to RNA-DNA hybrid).

    Techniques: Real-time Polymerase Chain Reaction, In Vitro, Standard Deviation

    p66/p51 HIV-1 reverse transcriptase containing an RNA/DNA hybrid. (A) Fingers, palm, thumb and connection subdomains are color coded blue, red, green and yellow, respectively, with the darker and lighter colors representing the p66 and p51 subunits, respectively. The p66 C-terminal RNaseH domain is depicted in gold. RNA and DNA strands of the hybrid are depicted as magenta and sand-colored spheres, respectively. (B) Close-up of the p66 RNaseH domain containing portions of the RNA/DNA hybrid described by Lapkouski et al. ]. Structural elements have been outlined, and catalytic residues (cyan) are: D 1 : Asp498; D 2 : Asp549; D 3 : Asp443; E: Glu478. RNA and DNA strands of the RNA/DNA hybrid are depicted in red and blue, respectively. RNaseH: Ribonuclease H.

    Journal: Future medicinal chemistry

    Article Title: Active site and allosteric inhibitors of the ribonuclease H activity of HIV reverse transcriptase

    doi: 10.4155/fmc.13.178

    Figure Lengend Snippet: p66/p51 HIV-1 reverse transcriptase containing an RNA/DNA hybrid. (A) Fingers, palm, thumb and connection subdomains are color coded blue, red, green and yellow, respectively, with the darker and lighter colors representing the p66 and p51 subunits, respectively. The p66 C-terminal RNaseH domain is depicted in gold. RNA and DNA strands of the hybrid are depicted as magenta and sand-colored spheres, respectively. (B) Close-up of the p66 RNaseH domain containing portions of the RNA/DNA hybrid described by Lapkouski et al. ]. Structural elements have been outlined, and catalytic residues (cyan) are: D 1 : Asp498; D 2 : Asp549; D 3 : Asp443; E: Glu478. RNA and DNA strands of the RNA/DNA hybrid are depicted in red and blue, respectively. RNaseH: Ribonuclease H.

    Article Snippet: The naphthyridine pharmacophore was originally described as an effective HIV-1 IN inhibitor by coordinating divalent ions at the active site [ ], and subsequently shown to inhibit HIV-1 RNaseH by Merck Research Laboratories [ ].

    Techniques: