rnase h Thermo Fisher Search Results


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  • 84
    Thermo Fisher rnaase h
    Rnaase H, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher ribonuclease h
    Two regions in  T. brucei  vtRNA are accessible for base-pairing interactions. A , diagram depicting the antisense oligodeoxyribonucleotides used in the RNase H cleavage assay. Shown is the antisense probe used for Northern blotting.  B , Northern blot analysis detecting vtRNA in the samples treated with RNase H and the indicated oligos. Controls without oligo (+ RNase H ) and without both oligo and RNase H (− RNase H ) are loaded at the beginning. Marker is a  32 P-labeled pBR322 DNA MspI digest.
    Ribonuclease H, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 643 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rnase h
    Two regions in  T. brucei  vtRNA are accessible for base-pairing interactions. A , diagram depicting the antisense oligodeoxyribonucleotides used in the RNase H cleavage assay. Shown is the antisense probe used for Northern blotting.  B , Northern blot analysis detecting vtRNA in the samples treated with RNase H and the indicated oligos. Controls without oligo (+ RNase H ) and without both oligo and RNase H (− RNase H ) are loaded at the beginning. Marker is a  32 P-labeled pBR322 DNA MspI digest.
    Rnase H, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7011 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript rnase h
    Two regions in  T. brucei  vtRNA are accessible for base-pairing interactions. A , diagram depicting the antisense oligodeoxyribonucleotides used in the RNase H cleavage assay. Shown is the antisense probe used for Northern blotting.  B , Northern blot analysis detecting vtRNA in the samples treated with RNase H and the indicated oligos. Controls without oligo (+ RNase H ) and without both oligo and RNase H (− RNase H ) are loaded at the beginning. Marker is a  32 P-labeled pBR322 DNA MspI digest.
    Superscript Rnase H, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rnase h buffer
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Rnase H Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Thermo Fisher thermoscript rnase h
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Thermoscript Rnase H, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ii rnase h
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Ii Rnase H, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher revertaid rnase h mbi fermentas
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Revertaid Rnase H Mbi Fermentas, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rnase h reverse transcriptase
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Rnase H Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 414 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rnase h reverse transcriptaseii kit
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Rnase H Reverse Transcriptaseii Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ambion rnase h
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Ambion Rnase H, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript ii rnaase h reverse transcriptase kit
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Superscript Ii Rnaase H Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 70/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript ii rnase h system
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Superscript Ii Rnase H System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher thermoscript rnase h reverse transcriptase
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Thermoscript Rnase H Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript rnase h reverse transcriptase
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Superscript Rnase H Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1531 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher thermoscripttm rnase h reverse transcriptase
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Thermoscripttm Rnase H Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher supercriptii rnase h reverse transcriptase
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Supercriptii Rnase H Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscriptiii rnase h reverse transcriptase
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Superscriptiii Rnase H Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher supersriptiii rnase h reverse transcriptase
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Supersriptiii Rnase H Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript ii rnase h kit
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Superscript Ii Rnase H Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher transcriptase mmulv rnase h minus
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Transcriptase Mmulv Rnase H Minus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher superscript ii rnase h negative
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Superscript Ii Rnase H Negative, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscripttm rnase h free reverse transcriptase
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Superscripttm Rnase H Free Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher superscript rnase h reverse transcriptase kit
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Superscript Rnase H Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii ribonuclease h reverse transcriptase kit
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Superscript Iii Ribonuclease H Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher novascript iii rnase h
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Novascript Iii Rnase H, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher surperscripttm ii rnase h reverse transcriptase
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Surperscripttm Ii Rnase H Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 76/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscripttm ii rnase h reverse transcriptase
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Superscripttm Ii Rnase H Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Thermo Fisher superscipt ii rnase h reverse transcriptase
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Superscipt Ii Rnase H Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher supersript ii rnase h reverse transcriptase
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Supersript Ii Rnase H Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/supersript ii rnase h reverse transcriptase/product/Thermo Fisher
    Average 79 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    supersript ii rnase h reverse transcriptase - by Bioz Stars, 2020-01
    79/100 stars
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    78
    Thermo Fisher super scripttm rnase h reverse transcriptase kit
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Super Scripttm Rnase H Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/super scripttm rnase h reverse transcriptase kit/product/Thermo Fisher
    Average 78 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    super scripttm rnase h reverse transcriptase kit - by Bioz Stars, 2020-01
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    Image Search Results


    Two regions in  T. brucei  vtRNA are accessible for base-pairing interactions. A , diagram depicting the antisense oligodeoxyribonucleotides used in the RNase H cleavage assay. Shown is the antisense probe used for Northern blotting.  B , Northern blot analysis detecting vtRNA in the samples treated with RNase H and the indicated oligos. Controls without oligo (+ RNase H ) and without both oligo and RNase H (− RNase H ) are loaded at the beginning. Marker is a  32 P-labeled pBR322 DNA MspI digest.

    Journal: The Journal of Biological Chemistry

    Article Title: The vault RNA of Trypanosoma brucei plays a role in the production of trans-spliced mRNA

    doi: 10.1074/jbc.RA119.008580

    Figure Lengend Snippet: Two regions in T. brucei vtRNA are accessible for base-pairing interactions. A , diagram depicting the antisense oligodeoxyribonucleotides used in the RNase H cleavage assay. Shown is the antisense probe used for Northern blotting. B , Northern blot analysis detecting vtRNA in the samples treated with RNase H and the indicated oligos. Controls without oligo (+ RNase H ) and without both oligo and RNase H (− RNase H ) are loaded at the beginning. Marker is a 32 P-labeled pBR322 DNA MspI digest.

    Article Snippet: After incubation of the mixtures for 10 min at room temperature, 2 units of RNase H (Invitrogen, catalog no. 18021071) were added, and the reactions were incubated at the same temperature for 1 h. TRIzol reagent (Invitrogen, catalog no. 15596026) was used to isolate the RNA from the reactions according to the manufacturer's instructions.

    Techniques: Cleavage Assay, Northern Blot, Marker, Labeling

    HPLC separation of products of miR191 cleavage by RNase H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).

    Journal: Nucleic Acids Research

    Article Title: 5?-O-Methylphosphonate nucleic acids--new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes

    doi: 10.1093/nar/gku125

    Figure Lengend Snippet: HPLC separation of products of miR191 cleavage by RNase H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).

    Article Snippet: RNase H from E. coli and RNase H buffer were purchased from Invitrogen (Coralville, IA, USA).

    Techniques: High Performance Liquid Chromatography, Spectroscopy

    Design of the SPR experiment for the study of RNase H activity. Oligonucleotide immobilization, which gave the reference level (shown as a dashed line), was followed by hybridization of the injected AO and immobilized probe. The solution of RNase H and the AO was injected immediately after the AO hybridization. Hydrolysis of the probe was observed as a decrease in the sensor response. The difference between the initial level and level after the RNase H injection was proportional to the amount of cleaved substrate.

    Journal: Nucleic Acids Research

    Article Title: 5?-O-Methylphosphonate nucleic acids--new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes

    doi: 10.1093/nar/gku125

    Figure Lengend Snippet: Design of the SPR experiment for the study of RNase H activity. Oligonucleotide immobilization, which gave the reference level (shown as a dashed line), was followed by hybridization of the injected AO and immobilized probe. The solution of RNase H and the AO was injected immediately after the AO hybridization. Hydrolysis of the probe was observed as a decrease in the sensor response. The difference between the initial level and level after the RNase H injection was proportional to the amount of cleaved substrate.

    Article Snippet: RNase H from E. coli and RNase H buffer were purchased from Invitrogen (Coralville, IA, USA).

    Techniques: SPR Assay, Activity Assay, Hybridization, Injection

    SPR measurements of the RNase H activity on the Amir*miR-191 complex. The sensor response to the hybridization of Amir to the immobilized Pr191 probe containing the miR-191 RNA sequence and the resulting Pr191 cleavage by RNase H is shown. Arrows indicate the injection of the respective solutions.

    Journal: Nucleic Acids Research

    Article Title: 5?-O-Methylphosphonate nucleic acids--new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes

    doi: 10.1093/nar/gku125

    Figure Lengend Snippet: SPR measurements of the RNase H activity on the Amir*miR-191 complex. The sensor response to the hybridization of Amir to the immobilized Pr191 probe containing the miR-191 RNA sequence and the resulting Pr191 cleavage by RNase H is shown. Arrows indicate the injection of the respective solutions.

    Article Snippet: RNase H from E. coli and RNase H buffer were purchased from Invitrogen (Coralville, IA, USA).

    Techniques: SPR Assay, Activity Assay, Hybridization, Sequencing, Injection