rnase h Search Results


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  • 95
    New England Biolabs ribonuclease h
    Ribonuclease H, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Thermo Fisher rnaase h
    Rnaase H, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Millipore rnaase h
    Rnaase H, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    TaKaRa rnase h
    Determination of specificity of binding of oligonucleotides designed based upon RT-ROL results to viral RNA by cleavage with RNase H. HIV-2 1-561 RNA was incubated in monomer buffer with the oligonucleotides indicated and RNase H. Digestion products were loaded onto a denaturing polyacrylamide gel and fragments were visualized by ethidium bromide staining. Lanes L: RNA size ladder (Ambion); Lane 1: complete reaction except with no oligonucleotide added; Lanes 2-8: complete reactions with oligonucleotide asROD32, asROD99, asROD172, asROD258, asROD309, asRODPBS, and asROD344 added, respectively; Lane 9: control reaction with the non-complementary sense oligonucleotide sROD228.
    Rnase H, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 452 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Beyotime ribonuclease h
    Determination of specificity of binding of oligonucleotides designed based upon RT-ROL results to viral RNA by cleavage with RNase H. HIV-2 1-561 RNA was incubated in monomer buffer with the oligonucleotides indicated and RNase H. Digestion products were loaded onto a denaturing polyacrylamide gel and fragments were visualized by ethidium bromide staining. Lanes L: RNA size ladder (Ambion); Lane 1: complete reaction except with no oligonucleotide added; Lanes 2-8: complete reactions with oligonucleotide asROD32, asROD99, asROD172, asROD258, asROD309, asRODPBS, and asROD344 added, respectively; Lane 9: control reaction with the non-complementary sense oligonucleotide sROD228.
    Ribonuclease H, supplied by Beyotime, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Roche ribonuclease h
    Nrl1 prevents R-loop accumulation. ( A ) Immunofluorescence analysis of RNA–DNA hybrids in chromosome spreads from WT (TH8342) and nrl1Δ (16581) using the mouse monoclonal S6.9 antibody. As negative control, the spreads were pre-treated with <t>RNase</t> H (+RNase H) before immunostaining as previously described ( 34 ). +IR: The cells were exposed to 100 Gy of IR before immunostaining. (B) Quantification of the R-loop positive nuclei in A. Mean and standard deviation were scored from triplicate experiments, n > 200. The asterisks (*) indicate significant differences compared with WT as determined by paired T-test (* P = 0.01, ** P = 0.003). ( C ) nrl1Δ becomes hypersensitive to bleocin in the absence of Rnh1 and Rnh201. Fivefold serial dilution of nrl1Δ (TH8341) rnh1Δ rnh201Δ (TH8743) and nrl1Δ rnh1Δ rnh201Δ (TH8904) in the absence and presence of bleocin (0.2 μg/ml).
    Ribonuclease H, supplied by Roche, used in various techniques. Bioz Stars score: 87/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Promega ribonuclease h
    Nrl1 prevents R-loop accumulation. ( A ) Immunofluorescence analysis of RNA–DNA hybrids in chromosome spreads from WT (TH8342) and nrl1Δ (16581) using the mouse monoclonal S6.9 antibody. As negative control, the spreads were pre-treated with <t>RNase</t> H (+RNase H) before immunostaining as previously described ( 34 ). +IR: The cells were exposed to 100 Gy of IR before immunostaining. (B) Quantification of the R-loop positive nuclei in A. Mean and standard deviation were scored from triplicate experiments, n > 200. The asterisks (*) indicate significant differences compared with WT as determined by paired T-test (* P = 0.01, ** P = 0.003). ( C ) nrl1Δ becomes hypersensitive to bleocin in the absence of Rnh1 and Rnh201. Fivefold serial dilution of nrl1Δ (TH8341) rnh1Δ rnh201Δ (TH8743) and nrl1Δ rnh1Δ rnh201Δ (TH8904) in the absence and presence of bleocin (0.2 μg/ml).
    Ribonuclease H, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher ribonuclease h
    Two regions in  T. brucei  vtRNA are accessible for base-pairing interactions. A , diagram depicting the antisense oligodeoxyribonucleotides used in the RNase H cleavage assay. Shown is the antisense probe used for Northern blotting.  B , Northern blot analysis detecting vtRNA in the samples treated with RNase H and the indicated oligos. Controls without oligo (+ RNase H ) and without both oligo and RNase H (− RNase H ) are loaded at the beginning. Marker is a  32 P-labeled pBR322 DNA MspI digest.
    Ribonuclease H, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 435 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Boehringer Mannheim rnase h
    Two regions in  T. brucei  vtRNA are accessible for base-pairing interactions. A , diagram depicting the antisense oligodeoxyribonucleotides used in the RNase H cleavage assay. Shown is the antisense probe used for Northern blotting.  B , Northern blot analysis detecting vtRNA in the samples treated with RNase H and the indicated oligos. Controls without oligo (+ RNase H ) and without both oligo and RNase H (− RNase H ) are loaded at the beginning. Marker is a  32 P-labeled pBR322 DNA MspI digest.
    Rnase H, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 91/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare rnase h
    Two regions in  T. brucei  vtRNA are accessible for base-pairing interactions. A , diagram depicting the antisense oligodeoxyribonucleotides used in the RNase H cleavage assay. Shown is the antisense probe used for Northern blotting.  B , Northern blot analysis detecting vtRNA in the samples treated with RNase H and the indicated oligos. Controls without oligo (+ RNase H ) and without both oligo and RNase H (− RNase H ) are loaded at the beginning. Marker is a  32 P-labeled pBR322 DNA MspI digest.
    Rnase H, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 95/100, based on 241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore rnase h
    Impaired EMCV replication in untreated Krebs-2 S10 extract programmed with the poly(A) tail-deficient EMCV RNA. ( A ) RNase H/oligo(dT) treatment of EMCV RNA. EMCV RNA that was treated with RNase H/oligo(dT) and blocked at the 3′ end with cordycepin
    Rnase H, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene rnase h
    Impaired EMCV replication in untreated Krebs-2 S10 extract programmed with the poly(A) tail-deficient EMCV RNA. ( A ) RNase H/oligo(dT) treatment of EMCV RNA. EMCV RNA that was treated with RNase H/oligo(dT) and blocked at the 3′ end with cordycepin
    Rnase H, supplied by Stratagene, used in various techniques. Bioz Stars score: 91/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rnase h
    Impaired EMCV replication in untreated Krebs-2 S10 extract programmed with the poly(A) tail-deficient EMCV RNA. ( A ) RNase H/oligo(dT) treatment of EMCV RNA. EMCV RNA that was treated with RNase H/oligo(dT) and blocked at the 3′ end with cordycepin
    Rnase H, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7011 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Epicentre Biotechnologies rnase h
    Impaired EMCV replication in untreated Krebs-2 S10 extract programmed with the poly(A) tail-deficient EMCV RNA. ( A ) RNase H/oligo(dT) treatment of EMCV RNA. EMCV RNA that was treated with RNase H/oligo(dT) and blocked at the 3′ end with cordycepin
    Rnase H, supplied by Epicentre Biotechnologies, used in various techniques. Bioz Stars score: 91/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Toyobo rnase h
    Impaired EMCV replication in untreated Krebs-2 S10 extract programmed with the poly(A) tail-deficient EMCV RNA. ( A ) RNase H/oligo(dT) treatment of EMCV RNA. EMCV RNA that was treated with RNase H/oligo(dT) and blocked at the 3′ end with cordycepin
    Rnase H, supplied by Toyobo, used in various techniques. Bioz Stars score: 91/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Enzymatics rnase h
    Impaired EMCV replication in untreated Krebs-2 S10 extract programmed with the poly(A) tail-deficient EMCV RNA. ( A ) RNase H/oligo(dT) treatment of EMCV RNA. EMCV RNA that was treated with RNase H/oligo(dT) and blocked at the 3′ end with cordycepin
    Rnase H, supplied by Enzymatics, used in various techniques. Bioz Stars score: 94/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc rnase h
    Impaired EMCV replication in untreated Krebs-2 S10 extract programmed with the poly(A) tail-deficient EMCV RNA. ( A ) RNase H/oligo(dT) treatment of EMCV RNA. EMCV RNA that was treated with RNase H/oligo(dT) and blocked at the 3′ end with cordycepin
    Rnase H, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 96/100, based on 572 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam rnase h
    Impaired EMCV replication in untreated Krebs-2 S10 extract programmed with the poly(A) tail-deficient EMCV RNA. ( A ) RNase H/oligo(dT) treatment of EMCV RNA. EMCV RNA that was treated with RNase H/oligo(dT) and blocked at the 3′ end with cordycepin
    Rnase H, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nugen rnase h
    Impaired EMCV replication in untreated Krebs-2 S10 extract programmed with the poly(A) tail-deficient EMCV RNA. ( A ) RNase H/oligo(dT) treatment of EMCV RNA. EMCV RNA that was treated with RNase H/oligo(dT) and blocked at the 3′ end with cordycepin
    Rnase H, supplied by Nugen, used in various techniques. Bioz Stars score: 91/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega superscript rnase h
    Impaired EMCV replication in untreated Krebs-2 S10 extract programmed with the poly(A) tail-deficient EMCV RNA. ( A ) RNase H/oligo(dT) treatment of EMCV RNA. EMCV RNA that was treated with RNase H/oligo(dT) and blocked at the 3′ end with cordycepin
    Superscript Rnase H, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript rnase h
    Impaired EMCV replication in untreated Krebs-2 S10 extract programmed with the poly(A) tail-deficient EMCV RNA. ( A ) RNase H/oligo(dT) treatment of EMCV RNA. EMCV RNA that was treated with RNase H/oligo(dT) and blocked at the 3′ end with cordycepin
    Superscript Rnase H, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rnase h buffer
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Rnase H Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Thermo Fisher thermoscript rnase h
    HPLC separation of products of miR191 cleavage by <t>RNase</t> H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).
    Thermoscript Rnase H, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    70
    Epicentre Biotechnologies thermostable rnase h
    SNP genotyping of 702 NOD2/CARD15 gene PCR products. Cleavage reactions contained 10 μL of PCR product and either 5 pmol (▲) of R702WPM or 5 pmol (●) of R702PW, and 2.5U of thermostable <t>RNase</t> H in 20 μL of cleavage buffer.
    Thermostable Rnase H, supplied by Epicentre Biotechnologies, used in various techniques. Bioz Stars score: 70/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    New England Biolabs hybridase thermostable rnase h
    SNP genotyping of 702 NOD2/CARD15 gene PCR products. Cleavage reactions contained 10 μL of PCR product and either 5 pmol (▲) of R702WPM or 5 pmol (●) of R702PW, and 2.5U of thermostable <t>RNase</t> H in 20 μL of cleavage buffer.
    Hybridase Thermostable Rnase H, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Roche rnase h endonuclease
    SNP genotyping of 702 NOD2/CARD15 gene PCR products. Cleavage reactions contained 10 μL of PCR product and either 5 pmol (▲) of R702WPM or 5 pmol (●) of R702PW, and 2.5U of thermostable <t>RNase</t> H in 20 μL of cleavage buffer.
    Rnase H Endonuclease, supplied by Roche, used in various techniques. Bioz Stars score: 83/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Horizon Discovery rnase h protection assays
    Prp43p is required for snoRNA-mediated methylation of C 2337 in 25S rRNA at the 27S pre-rRNA stage. 2′- O -methylation by the box C/D snoRNA snR64 at C 2337 ). The unmodified 5.8S residue U 68 was assayed as a positive control for cleavage. The first two lanes represent negative controls. RNA from PRP43 or prp43-Q423N strains, shifted 2 h at 15°C, was <t>RNase</t> H digested and analyzed by Northern using probe o6. The schematic shown at the top indicates the position of C 2337 , U 68 , and the o6 probe in 35S; the other schematics symbolize the cleavage products, marked by arrows and labeled with the pre-rRNA from which it derives. The asterisk marks nonspecific hybridization.
    Rnase H Protection Assays, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 75/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega rnase h digestion step
    Prp43p is required for snoRNA-mediated methylation of C 2337 in 25S rRNA at the 27S pre-rRNA stage. 2′- O -methylation by the box C/D snoRNA snR64 at C 2337 ). The unmodified 5.8S residue U 68 was assayed as a positive control for cleavage. The first two lanes represent negative controls. RNA from PRP43 or prp43-Q423N strains, shifted 2 h at 15°C, was <t>RNase</t> H digested and analyzed by Northern using probe o6. The schematic shown at the top indicates the position of C 2337 , U 68 , and the o6 probe in 35S; the other schematics symbolize the cleavage products, marked by arrows and labeled with the pre-rRNA from which it derives. The asterisk marks nonspecific hybridization.
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    70
    Meridian Life Science bioscript rnase h minus
    Prp43p is required for snoRNA-mediated methylation of C 2337 in 25S rRNA at the 27S pre-rRNA stage. 2′- O -methylation by the box C/D snoRNA snR64 at C 2337 ). The unmodified 5.8S residue U 68 was assayed as a positive control for cleavage. The first two lanes represent negative controls. RNA from PRP43 or prp43-Q423N strains, shifted 2 h at 15°C, was <t>RNase</t> H digested and analyzed by Northern using probe o6. The schematic shown at the top indicates the position of C 2337 , U 68 , and the o6 probe in 35S; the other schematics symbolize the cleavage products, marked by arrows and labeled with the pre-rRNA from which it derives. The asterisk marks nonspecific hybridization.
    Bioscript Rnase H Minus, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 70/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Determination of specificity of binding of oligonucleotides designed based upon RT-ROL results to viral RNA by cleavage with RNase H. HIV-2 1-561 RNA was incubated in monomer buffer with the oligonucleotides indicated and RNase H. Digestion products were loaded onto a denaturing polyacrylamide gel and fragments were visualized by ethidium bromide staining. Lanes L: RNA size ladder (Ambion); Lane 1: complete reaction except with no oligonucleotide added; Lanes 2-8: complete reactions with oligonucleotide asROD32, asROD99, asROD172, asROD258, asROD309, asRODPBS, and asROD344 added, respectively; Lane 9: control reaction with the non-complementary sense oligonucleotide sROD228.

    Journal: Antisense & nucleic acid drug development

    Article Title: Elucidation and characterization of oligonucleotide-accessible sites on HIV-2 leader region RNA

    doi: 10.1089/108729003764097331

    Figure Lengend Snippet: Determination of specificity of binding of oligonucleotides designed based upon RT-ROL results to viral RNA by cleavage with RNase H. HIV-2 1-561 RNA was incubated in monomer buffer with the oligonucleotides indicated and RNase H. Digestion products were loaded onto a denaturing polyacrylamide gel and fragments were visualized by ethidium bromide staining. Lanes L: RNA size ladder (Ambion); Lane 1: complete reaction except with no oligonucleotide added; Lanes 2-8: complete reactions with oligonucleotide asROD32, asROD99, asROD172, asROD258, asROD309, asRODPBS, and asROD344 added, respectively; Lane 9: control reaction with the non-complementary sense oligonucleotide sROD228.

    Article Snippet: Then RNase H (1U; Takara) was added and allowed to incubate at 24°C for 5 minutes.

    Techniques: Binding Assay, Incubation, Staining

    Nrl1 prevents R-loop accumulation. ( A ) Immunofluorescence analysis of RNA–DNA hybrids in chromosome spreads from WT (TH8342) and nrl1Δ (16581) using the mouse monoclonal S6.9 antibody. As negative control, the spreads were pre-treated with RNase H (+RNase H) before immunostaining as previously described ( 34 ). +IR: The cells were exposed to 100 Gy of IR before immunostaining. (B) Quantification of the R-loop positive nuclei in A. Mean and standard deviation were scored from triplicate experiments, n > 200. The asterisks (*) indicate significant differences compared with WT as determined by paired T-test (* P = 0.01, ** P = 0.003). ( C ) nrl1Δ becomes hypersensitive to bleocin in the absence of Rnh1 and Rnh201. Fivefold serial dilution of nrl1Δ (TH8341) rnh1Δ rnh201Δ (TH8743) and nrl1Δ rnh1Δ rnh201Δ (TH8904) in the absence and presence of bleocin (0.2 μg/ml).

    Journal: Nucleic Acids Research

    Article Title: The spliceosome-associated protein Nrl1 suppresses homologous recombination-dependent R-loop formation in fission yeast

    doi: 10.1093/nar/gkv1473

    Figure Lengend Snippet: Nrl1 prevents R-loop accumulation. ( A ) Immunofluorescence analysis of RNA–DNA hybrids in chromosome spreads from WT (TH8342) and nrl1Δ (16581) using the mouse monoclonal S6.9 antibody. As negative control, the spreads were pre-treated with RNase H (+RNase H) before immunostaining as previously described ( 34 ). +IR: The cells were exposed to 100 Gy of IR before immunostaining. (B) Quantification of the R-loop positive nuclei in A. Mean and standard deviation were scored from triplicate experiments, n > 200. The asterisks (*) indicate significant differences compared with WT as determined by paired T-test (* P = 0.01, ** P = 0.003). ( C ) nrl1Δ becomes hypersensitive to bleocin in the absence of Rnh1 and Rnh201. Fivefold serial dilution of nrl1Δ (TH8341) rnh1Δ rnh201Δ (TH8743) and nrl1Δ rnh1Δ rnh201Δ (TH8904) in the absence and presence of bleocin (0.2 μg/ml).

    Article Snippet: For RNase H controls, slides were incubated with 2 U of RNase H (Roche) and 1 μg RNase A (Roche) in PBS buffer for 2 h prior to antibody treatment.

    Techniques: Immunofluorescence, Negative Control, Immunostaining, Standard Deviation, Serial Dilution

    Two regions in  T. brucei  vtRNA are accessible for base-pairing interactions. A , diagram depicting the antisense oligodeoxyribonucleotides used in the RNase H cleavage assay. Shown is the antisense probe used for Northern blotting.  B , Northern blot analysis detecting vtRNA in the samples treated with RNase H and the indicated oligos. Controls without oligo (+ RNase H ) and without both oligo and RNase H (− RNase H ) are loaded at the beginning. Marker is a  32 P-labeled pBR322 DNA MspI digest.

    Journal: The Journal of Biological Chemistry

    Article Title: The vault RNA of Trypanosoma brucei plays a role in the production of trans-spliced mRNA

    doi: 10.1074/jbc.RA119.008580

    Figure Lengend Snippet: Two regions in T. brucei vtRNA are accessible for base-pairing interactions. A , diagram depicting the antisense oligodeoxyribonucleotides used in the RNase H cleavage assay. Shown is the antisense probe used for Northern blotting. B , Northern blot analysis detecting vtRNA in the samples treated with RNase H and the indicated oligos. Controls without oligo (+ RNase H ) and without both oligo and RNase H (− RNase H ) are loaded at the beginning. Marker is a 32 P-labeled pBR322 DNA MspI digest.

    Article Snippet: After incubation of the mixtures for 10 min at room temperature, 2 units of RNase H (Invitrogen, catalog no. 18021071) were added, and the reactions were incubated at the same temperature for 1 h. TRIzol reagent (Invitrogen, catalog no. 15596026) was used to isolate the RNA from the reactions according to the manufacturer's instructions.

    Techniques: Cleavage Assay, Northern Blot, Marker, Labeling

    Impaired EMCV replication in untreated Krebs-2 S10 extract programmed with the poly(A) tail-deficient EMCV RNA. ( A ) RNase H/oligo(dT) treatment of EMCV RNA. EMCV RNA that was treated with RNase H/oligo(dT) and blocked at the 3′ end with cordycepin

    Journal:

    Article Title: Stimulation of picornavirus replication by the poly(A) tail in a cell-free extract is largely independent of the poly(A) binding protein (PABP)

    doi: 10.1261/rna.606407

    Figure Lengend Snippet: Impaired EMCV replication in untreated Krebs-2 S10 extract programmed with the poly(A) tail-deficient EMCV RNA. ( A ) RNase H/oligo(dT) treatment of EMCV RNA. EMCV RNA that was treated with RNase H/oligo(dT) and blocked at the 3′ end with cordycepin

    Article Snippet: Ten microliters of 5× RNase H buffer (100 mM Tris-HCl, pH 7.8, 40 mM MgCl2 , 5 mM DTT, and 10% glycerol) and 1 μL (2 U) of RNase H (Sigma) was added at room temperature, and the reaction mixture was incubated for 20 min at 37°C.

    Techniques:

    HPLC separation of products of miR191 cleavage by RNase H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).

    Journal: Nucleic Acids Research

    Article Title: 5?-O-Methylphosphonate nucleic acids--new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes

    doi: 10.1093/nar/gku125

    Figure Lengend Snippet: HPLC separation of products of miR191 cleavage by RNase H E. coli . miR191 was hybridized with various AOs (Amir-0–Amir-4). The enzymatic reaction was stopped at specific times by the addition of EDTA to the solution. The numbers above selected peaks indicate products identified with MALDI TOF spectroscopy: ‘a’ indicates fragments of the same molecular mass, and any combination of them is possible. Sections of chromatograms corresponding to Amir and hybrid duplexes are shown in Supplementary materials ( Supplementary Figure S3 ).

    Article Snippet: RNase H from E. coli and RNase H buffer were purchased from Invitrogen (Coralville, IA, USA).

    Techniques: High Performance Liquid Chromatography, Spectroscopy

    Design of the SPR experiment for the study of RNase H activity. Oligonucleotide immobilization, which gave the reference level (shown as a dashed line), was followed by hybridization of the injected AO and immobilized probe. The solution of RNase H and the AO was injected immediately after the AO hybridization. Hydrolysis of the probe was observed as a decrease in the sensor response. The difference between the initial level and level after the RNase H injection was proportional to the amount of cleaved substrate.

    Journal: Nucleic Acids Research

    Article Title: 5?-O-Methylphosphonate nucleic acids--new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes

    doi: 10.1093/nar/gku125

    Figure Lengend Snippet: Design of the SPR experiment for the study of RNase H activity. Oligonucleotide immobilization, which gave the reference level (shown as a dashed line), was followed by hybridization of the injected AO and immobilized probe. The solution of RNase H and the AO was injected immediately after the AO hybridization. Hydrolysis of the probe was observed as a decrease in the sensor response. The difference between the initial level and level after the RNase H injection was proportional to the amount of cleaved substrate.

    Article Snippet: RNase H from E. coli and RNase H buffer were purchased from Invitrogen (Coralville, IA, USA).

    Techniques: SPR Assay, Activity Assay, Hybridization, Injection

    SPR measurements of the RNase H activity on the Amir*miR-191 complex. The sensor response to the hybridization of Amir to the immobilized Pr191 probe containing the miR-191 RNA sequence and the resulting Pr191 cleavage by RNase H is shown. Arrows indicate the injection of the respective solutions.

    Journal: Nucleic Acids Research

    Article Title: 5?-O-Methylphosphonate nucleic acids--new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes

    doi: 10.1093/nar/gku125

    Figure Lengend Snippet: SPR measurements of the RNase H activity on the Amir*miR-191 complex. The sensor response to the hybridization of Amir to the immobilized Pr191 probe containing the miR-191 RNA sequence and the resulting Pr191 cleavage by RNase H is shown. Arrows indicate the injection of the respective solutions.

    Article Snippet: RNase H from E. coli and RNase H buffer were purchased from Invitrogen (Coralville, IA, USA).

    Techniques: SPR Assay, Activity Assay, Hybridization, Sequencing, Injection

    SNP genotyping of 702 NOD2/CARD15 gene PCR products. Cleavage reactions contained 10 μL of PCR product and either 5 pmol (▲) of R702WPM or 5 pmol (●) of R702PW, and 2.5U of thermostable RNase H in 20 μL of cleavage buffer.

    Journal:

    Article Title: SNP Analysis Using CataCleave Probes

    doi: 10.1002/jcla.20240

    Figure Lengend Snippet: SNP genotyping of 702 NOD2/CARD15 gene PCR products. Cleavage reactions contained 10 μL of PCR product and either 5 pmol (▲) of R702WPM or 5 pmol (●) of R702PW, and 2.5U of thermostable RNase H in 20 μL of cleavage buffer.

    Article Snippet: Thermostable RNase H was purchased from Epicentre Biotechnologies (Madison, WI).

    Techniques: Polymerase Chain Reaction

    Prp43p is required for snoRNA-mediated methylation of C 2337 in 25S rRNA at the 27S pre-rRNA stage. 2′- O -methylation by the box C/D snoRNA snR64 at C 2337 ). The unmodified 5.8S residue U 68 was assayed as a positive control for cleavage. The first two lanes represent negative controls. RNA from PRP43 or prp43-Q423N strains, shifted 2 h at 15°C, was RNase H digested and analyzed by Northern using probe o6. The schematic shown at the top indicates the position of C 2337 , U 68 , and the o6 probe in 35S; the other schematics symbolize the cleavage products, marked by arrows and labeled with the pre-rRNA from which it derives. The asterisk marks nonspecific hybridization.

    Journal: Molecular and Cellular Biology

    Article Title: The Splicing Factor Prp43p, a DEAH Box ATPase, Functions in Ribosome Biogenesis

    doi: 10.1128/MCB.26.2.513-522.2006

    Figure Lengend Snippet: Prp43p is required for snoRNA-mediated methylation of C 2337 in 25S rRNA at the 27S pre-rRNA stage. 2′- O -methylation by the box C/D snoRNA snR64 at C 2337 ). The unmodified 5.8S residue U 68 was assayed as a positive control for cleavage. The first two lanes represent negative controls. RNA from PRP43 or prp43-Q423N strains, shifted 2 h at 15°C, was RNase H digested and analyzed by Northern using probe o6. The schematic shown at the top indicates the position of C 2337 , U 68 , and the o6 probe in 35S; the other schematics symbolize the cleavage products, marked by arrows and labeled with the pre-rRNA from which it derives. The asterisk marks nonspecific hybridization.

    Article Snippet: Chimeric 2′ O -methyl oligonucleotides for RNase H protection assays (Dharmacon) were as follows: C2337 , 5′mGmAmUmAmGdGdGdAdCmAmGmUmGmGmGmAmAmUmCmUmC mG; and U68 , 5′mUmUmCmAdCdAdTdTmAmCmGmUmAmUmCmGmCmAmUmUmU.

    Techniques: Methylation, Positive Control, Northern Blot, Labeling, Hybridization