rnase free dnase i Roche Search Results


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  • 97
    Roche rnase free dnase i
    Rnase Free Dnase I, supplied by Roche, used in various techniques. Bioz Stars score: 97/100, based on 3548 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche rq1 rnase free dnase i
    Rq1 Rnase Free Dnase I, supplied by Roche, used in various techniques. Bioz Stars score: 87/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche recombinant rnase free dnase i
    Uncapped, polyadenylated viral transcript induced IFNB gene expression. (A-C) Total RNA of mock treated or NDV-infected (12 hpi, MOI = 1) HeLa cells (A), RSV-infected (60 hpi, MOI = 1) HEp-2 cells (B), or VSV-infected (12 hpi, MOI = 1) HeLa cells (C) was separated into poly(A) - and poly(A) + RNA fractions by oligo(dT)-combined latex beads and transfected into MEFs (1×10 5 cells were transfected with 200 ng RNA). Ifnb mRNA expression levels were measured by RT-qPCR. Data are represented as means ±SD. (D and E) The Poly(A) + RNA fraction from NDV-infected (12 hpi, MOI = 1) HeLa cells was mock treated (NT) or treated with <t>RNase</t> III, DNase I, CIAP (D), or 5’-capping enzyme of Vaccinia virus (E) and then transfected to MEFs (1×10 5 cells were transfected with 200 ng RNA). Ifnb mRNA expression levels were measured by RT-qPCR. Data are represented as means ±SD (t-test: **p
    Recombinant Rnase Free Dnase I, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche ribonuclease free deoxyribonuclease dnase i
    Identification and functional analysis of an evolutionarily conserved GRE/MRE located at −5.3 kb in the mouse (−4.6 kb in the human) Klf9 gene. A, <t>DNAse</t> I protection assay with the hGR-DBD of the evolutionary conserved 179-bp fragment
    Ribonuclease Free Deoxyribonuclease Dnase I, supplied by Roche, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche rnase free dnase i recombinant kit
    Identification and functional analysis of an evolutionarily conserved GRE/MRE located at −5.3 kb in the mouse (−4.6 kb in the human) Klf9 gene. A, <t>DNAse</t> I protection assay with the hGR-DBD of the evolutionary conserved 179-bp fragment
    Rnase Free Dnase I Recombinant Kit, supplied by Roche, used in various techniques. Bioz Stars score: 83/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche rnase free bovine pancreatic dnase i
    Identification and functional analysis of an evolutionarily conserved GRE/MRE located at −5.3 kb in the mouse (−4.6 kb in the human) Klf9 gene. A, <t>DNAse</t> I protection assay with the hGR-DBD of the evolutionary conserved 179-bp fragment
    Rnase Free Bovine Pancreatic Dnase I, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche dnase i
    <t>DNase</t> I footprinting of the yefM-yoeB promoter-operator region. Footprinting reactions were performed as outlined in Materials and Methods using PCR fragments biotinylated at the 5′ ends of either upper or lower strands. YefM concentrations (μM, left to right): 0, 0.01, 0.025, 0.05, 0.1, 0.25, 0.5, 1.0, 2.5 and 5.0. YefM–YoeB–His 6 concentrations (μM, left to right): 0, 0.007, 0.018, 0.036, 0.072, 0.18, 0.36, 0.72, 1.8 and 3.6. The locations of the L and S repeats are marked by inverted arrows. Shaded boxes denote the regions protected from DNase I digestion by YefM and YefM–YoeB–His 6 . A + G, Maxam–Gilbert sequencing reactions. A position on the lower strand that is hypersenstive to DNase I cleavage in the presence of YefM and YefM–YoeB–His 6 is highlighted by the star. The relative dispositions of regions on the upper and lower strands that are protected from DNase I digestion and other features of the yefM-yoeB promoter–operator region are illustrated in the lower panel.
    Dnase I, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 25266 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Uncapped, polyadenylated viral transcript induced IFNB gene expression. (A-C) Total RNA of mock treated or NDV-infected (12 hpi, MOI = 1) HeLa cells (A), RSV-infected (60 hpi, MOI = 1) HEp-2 cells (B), or VSV-infected (12 hpi, MOI = 1) HeLa cells (C) was separated into poly(A) - and poly(A) + RNA fractions by oligo(dT)-combined latex beads and transfected into MEFs (1×10 5 cells were transfected with 200 ng RNA). Ifnb mRNA expression levels were measured by RT-qPCR. Data are represented as means ±SD. (D and E) The Poly(A) + RNA fraction from NDV-infected (12 hpi, MOI = 1) HeLa cells was mock treated (NT) or treated with RNase III, DNase I, CIAP (D), or 5’-capping enzyme of Vaccinia virus (E) and then transfected to MEFs (1×10 5 cells were transfected with 200 ng RNA). Ifnb mRNA expression levels were measured by RT-qPCR. Data are represented as means ±SD (t-test: **p

    Journal: PLoS Pathogens

    Article Title: Leader-Containing Uncapped Viral Transcript Activates RIG-I in Antiviral Stress Granules

    doi: 10.1371/journal.ppat.1005444

    Figure Lengend Snippet: Uncapped, polyadenylated viral transcript induced IFNB gene expression. (A-C) Total RNA of mock treated or NDV-infected (12 hpi, MOI = 1) HeLa cells (A), RSV-infected (60 hpi, MOI = 1) HEp-2 cells (B), or VSV-infected (12 hpi, MOI = 1) HeLa cells (C) was separated into poly(A) - and poly(A) + RNA fractions by oligo(dT)-combined latex beads and transfected into MEFs (1×10 5 cells were transfected with 200 ng RNA). Ifnb mRNA expression levels were measured by RT-qPCR. Data are represented as means ±SD. (D and E) The Poly(A) + RNA fraction from NDV-infected (12 hpi, MOI = 1) HeLa cells was mock treated (NT) or treated with RNase III, DNase I, CIAP (D), or 5’-capping enzyme of Vaccinia virus (E) and then transfected to MEFs (1×10 5 cells were transfected with 200 ng RNA). Ifnb mRNA expression levels were measured by RT-qPCR. Data are represented as means ±SD (t-test: **p

    Article Snippet: RNA samples were treated with 1 U of ShortCut RNase III (New England Biolabs), 1 U of RNase-free DNase I recombinant (Roche), and 15 U of Calf Intestine Alkaline Phosphatase (Takara) at 37°C for 30 minutes, or 10 U of Vaccinia Capping Enzyme (New England Biolabs) according to the manufacturer’s instruction.

    Techniques: Expressing, Infection, Transfection, Quantitative RT-PCR, T-Test

    Identification and functional analysis of an evolutionarily conserved GRE/MRE located at −5.3 kb in the mouse (−4.6 kb in the human) Klf9 gene. A, DNAse I protection assay with the hGR-DBD of the evolutionary conserved 179-bp fragment

    Journal: Endocrinology

    Article Title: Molecular Basis for Glucocorticoid Induction of the Kr?ppel-Like Factor 9 Gene in Hippocampal Neurons

    doi: 10.1210/en.2012-1303

    Figure Lengend Snippet: Identification and functional analysis of an evolutionarily conserved GRE/MRE located at −5.3 kb in the mouse (−4.6 kb in the human) Klf9 gene. A, DNAse I protection assay with the hGR-DBD of the evolutionary conserved 179-bp fragment

    Article Snippet: We treated 1 μg of total RNA with 20 U of ribonuclease-free deoxyribonuclease (DNAse) I (Roche, Indianapolis, IN) ( ) before cDNA synthesis with the High Capacity Reverse Transcription kit (Invitrogen) with or without the addition of RT.

    Techniques: Functional Assay

    DNase I footprinting of the yefM-yoeB promoter-operator region. Footprinting reactions were performed as outlined in Materials and Methods using PCR fragments biotinylated at the 5′ ends of either upper or lower strands. YefM concentrations (μM, left to right): 0, 0.01, 0.025, 0.05, 0.1, 0.25, 0.5, 1.0, 2.5 and 5.0. YefM–YoeB–His 6 concentrations (μM, left to right): 0, 0.007, 0.018, 0.036, 0.072, 0.18, 0.36, 0.72, 1.8 and 3.6. The locations of the L and S repeats are marked by inverted arrows. Shaded boxes denote the regions protected from DNase I digestion by YefM and YefM–YoeB–His 6 . A + G, Maxam–Gilbert sequencing reactions. A position on the lower strand that is hypersenstive to DNase I cleavage in the presence of YefM and YefM–YoeB–His 6 is highlighted by the star. The relative dispositions of regions on the upper and lower strands that are protected from DNase I digestion and other features of the yefM-yoeB promoter–operator region are illustrated in the lower panel.

    Journal: Nucleic Acids Research

    Article Title: Toxin-antitoxin regulation: bimodal interaction of YefM-YoeB with paired DNA palindromes exerts transcriptional autorepression

    doi: 10.1093/nar/gkl1028

    Figure Lengend Snippet: DNase I footprinting of the yefM-yoeB promoter-operator region. Footprinting reactions were performed as outlined in Materials and Methods using PCR fragments biotinylated at the 5′ ends of either upper or lower strands. YefM concentrations (μM, left to right): 0, 0.01, 0.025, 0.05, 0.1, 0.25, 0.5, 1.0, 2.5 and 5.0. YefM–YoeB–His 6 concentrations (μM, left to right): 0, 0.007, 0.018, 0.036, 0.072, 0.18, 0.36, 0.72, 1.8 and 3.6. The locations of the L and S repeats are marked by inverted arrows. Shaded boxes denote the regions protected from DNase I digestion by YefM and YefM–YoeB–His 6 . A + G, Maxam–Gilbert sequencing reactions. A position on the lower strand that is hypersenstive to DNase I cleavage in the presence of YefM and YefM–YoeB–His 6 is highlighted by the star. The relative dispositions of regions on the upper and lower strands that are protected from DNase I digestion and other features of the yefM-yoeB promoter–operator region are illustrated in the lower panel.

    Article Snippet: Each reaction was treated with 0.0075 U of DNase I (Roche, RNase free, 10 U/μl diluted in buffer [20 mM Tris–HCl (pH 7.5), 50 mM NaCl, 7.5 mM MgCl2 and 5 mM CaCl2 ]) for 45 s at 22°C.

    Techniques: Footprinting, Polymerase Chain Reaction, Sequencing