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  • 88
    Qiagen qiagen ribonuclease a
    Qiagen Ribonuclease A, supplied by Qiagen, used in various techniques. Bioz Stars score: 88/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Gentra Systems rnase a
    Rnase A, supplied by Gentra Systems, used in various techniques. Bioz Stars score: 93/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Qiagen rnase a
    Rnase A, supplied by Qiagen, used in various techniques. Bioz Stars score: 98/100, based on 7734 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Qiagen rnase a inhibitor
    Construction of recombinant E . coli expressing dsRNA specific to SeCHY2 (dsSeCHY2). (A) Location of dsSeCHY2 in cDNA of SeCHY2. (B) Directional cloning of dsSeCHY2 into L4440 vector using two restriction sites of Hind III and Spe I. The cloning site was located between two opposite T7 promoters. Recombinant vector was screened after transformation into E . coli in the presence of ampicillin antibiotics. LacZ promoter (ʻlacZʼ) was then induced by IPTG to express T7 RNA polymerase (ʻT7 RPʼ) which recognized two T7 promoters and transcribed dsSeCHY2 in both directions. (C) Resulting dsSeCHY2 and its resistance to <t>RNase</t> A treatment. ʻWildʼ and ʻRCʼ represent non-recombinant and recombinant HT115 bacteria, respectively. ʻMʼ represents DNA marker.
    Rnase A Inhibitor, supplied by Qiagen, used in various techniques. Bioz Stars score: 82/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Qiagen rnase a 25units
    Construction of recombinant E . coli expressing dsRNA specific to SeCHY2 (dsSeCHY2). (A) Location of dsSeCHY2 in cDNA of SeCHY2. (B) Directional cloning of dsSeCHY2 into L4440 vector using two restriction sites of Hind III and Spe I. The cloning site was located between two opposite T7 promoters. Recombinant vector was screened after transformation into E . coli in the presence of ampicillin antibiotics. LacZ promoter (ʻlacZʼ) was then induced by IPTG to express T7 RNA polymerase (ʻT7 RPʼ) which recognized two T7 promoters and transcribed dsSeCHY2 in both directions. (C) Resulting dsSeCHY2 and its resistance to <t>RNase</t> A treatment. ʻWildʼ and ʻRCʼ represent non-recombinant and recombinant HT115 bacteria, respectively. ʻMʼ represents DNA marker.
    Rnase A 25units, supplied by Qiagen, used in various techniques. Bioz Stars score: 82/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Qiagen dnase free rnase a qiaquick pcr purification kit
    Construction of recombinant E . coli expressing dsRNA specific to SeCHY2 (dsSeCHY2). (A) Location of dsSeCHY2 in cDNA of SeCHY2. (B) Directional cloning of dsSeCHY2 into L4440 vector using two restriction sites of Hind III and Spe I. The cloning site was located between two opposite T7 promoters. Recombinant vector was screened after transformation into E . coli in the presence of ampicillin antibiotics. LacZ promoter (ʻlacZʼ) was then induced by IPTG to express T7 RNA polymerase (ʻT7 RPʼ) which recognized two T7 promoters and transcribed dsSeCHY2 in both directions. (C) Resulting dsSeCHY2 and its resistance to <t>RNase</t> A treatment. ʻWildʼ and ʻRCʼ represent non-recombinant and recombinant HT115 bacteria, respectively. ʻMʼ represents DNA marker.
    Dnase Free Rnase A Qiaquick Pcr Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Qiagen p1 buffer
    Construction of recombinant E . coli expressing dsRNA specific to SeCHY2 (dsSeCHY2). (A) Location of dsSeCHY2 in cDNA of SeCHY2. (B) Directional cloning of dsSeCHY2 into L4440 vector using two restriction sites of Hind III and Spe I. The cloning site was located between two opposite T7 promoters. Recombinant vector was screened after transformation into E . coli in the presence of ampicillin antibiotics. LacZ promoter (ʻlacZʼ) was then induced by IPTG to express T7 RNA polymerase (ʻT7 RPʼ) which recognized two T7 promoters and transcribed dsSeCHY2 in both directions. (C) Resulting dsSeCHY2 and its resistance to <t>RNase</t> A treatment. ʻWildʼ and ʻRCʼ represent non-recombinant and recombinant HT115 bacteria, respectively. ʻMʼ represents DNA marker.
    P1 Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 93/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen dneasy plant kit
    Construction of recombinant E . coli expressing dsRNA specific to SeCHY2 (dsSeCHY2). (A) Location of dsSeCHY2 in cDNA of SeCHY2. (B) Directional cloning of dsSeCHY2 into L4440 vector using two restriction sites of Hind III and Spe I. The cloning site was located between two opposite T7 promoters. Recombinant vector was screened after transformation into E . coli in the presence of ampicillin antibiotics. LacZ promoter (ʻlacZʼ) was then induced by IPTG to express T7 RNA polymerase (ʻT7 RPʼ) which recognized two T7 promoters and transcribed dsSeCHY2 in both directions. (C) Resulting dsSeCHY2 and its resistance to <t>RNase</t> A treatment. ʻWildʼ and ʻRCʼ represent non-recombinant and recombinant HT115 bacteria, respectively. ʻMʼ represents DNA marker.
    Dneasy Plant Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1071 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen dneasy plant mini kit
    Construction of recombinant E . coli expressing dsRNA specific to SeCHY2 (dsSeCHY2). (A) Location of dsSeCHY2 in cDNA of SeCHY2. (B) Directional cloning of dsSeCHY2 into L4440 vector using two restriction sites of Hind III and Spe I. The cloning site was located between two opposite T7 promoters. Recombinant vector was screened after transformation into E . coli in the presence of ampicillin antibiotics. LacZ promoter (ʻlacZʼ) was then induced by IPTG to express T7 RNA polymerase (ʻT7 RPʼ) which recognized two T7 promoters and transcribed dsSeCHY2 in both directions. (C) Resulting dsSeCHY2 and its resistance to <t>RNase</t> A treatment. ʻWildʼ and ʻRCʼ represent non-recombinant and recombinant HT115 bacteria, respectively. ʻMʼ represents DNA marker.
    Dneasy Plant Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 13920 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen dneasy tissue kit
    Construction of recombinant E . coli expressing dsRNA specific to SeCHY2 (dsSeCHY2). (A) Location of dsSeCHY2 in cDNA of SeCHY2. (B) Directional cloning of dsSeCHY2 into L4440 vector using two restriction sites of Hind III and Spe I. The cloning site was located between two opposite T7 promoters. Recombinant vector was screened after transformation into E . coli in the presence of ampicillin antibiotics. LacZ promoter (ʻlacZʼ) was then induced by IPTG to express T7 RNA polymerase (ʻT7 RPʼ) which recognized two T7 promoters and transcribed dsSeCHY2 in both directions. (C) Resulting dsSeCHY2 and its resistance to <t>RNase</t> A treatment. ʻWildʼ and ʻRCʼ represent non-recombinant and recombinant HT115 bacteria, respectively. ʻMʼ represents DNA marker.
    Dneasy Tissue Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 14264 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen puregene cell kit
    Construction of recombinant E . coli expressing dsRNA specific to SeCHY2 (dsSeCHY2). (A) Location of dsSeCHY2 in cDNA of SeCHY2. (B) Directional cloning of dsSeCHY2 into L4440 vector using two restriction sites of Hind III and Spe I. The cloning site was located between two opposite T7 promoters. Recombinant vector was screened after transformation into E . coli in the presence of ampicillin antibiotics. LacZ promoter (ʻlacZʼ) was then induced by IPTG to express T7 RNA polymerase (ʻT7 RPʼ) which recognized two T7 promoters and transcribed dsSeCHY2 in both directions. (C) Resulting dsSeCHY2 and its resistance to <t>RNase</t> A treatment. ʻWildʼ and ʻRCʼ represent non-recombinant and recombinant HT115 bacteria, respectively. ʻMʼ represents DNA marker.
    Puregene Cell Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 917 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen minelute pcr purification kit
    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and <t>PCR</t> Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, <t>MinElute</t> PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Minelute Pcr Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 15587 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Construction of recombinant E . coli expressing dsRNA specific to SeCHY2 (dsSeCHY2). (A) Location of dsSeCHY2 in cDNA of SeCHY2. (B) Directional cloning of dsSeCHY2 into L4440 vector using two restriction sites of Hind III and Spe I. The cloning site was located between two opposite T7 promoters. Recombinant vector was screened after transformation into E . coli in the presence of ampicillin antibiotics. LacZ promoter (ʻlacZʼ) was then induced by IPTG to express T7 RNA polymerase (ʻT7 RPʼ) which recognized two T7 promoters and transcribed dsSeCHY2 in both directions. (C) Resulting dsSeCHY2 and its resistance to RNase A treatment. ʻWildʼ and ʻRCʼ represent non-recombinant and recombinant HT115 bacteria, respectively. ʻMʼ represents DNA marker.

    Journal: PLoS ONE

    Article Title: Optimization of recombinant bacteria expressing dsRNA to enhance insecticidal activity against a lepidopteran insect, Spodoptera exigua

    doi: 10.1371/journal.pone.0183054

    Figure Lengend Snippet: Construction of recombinant E . coli expressing dsRNA specific to SeCHY2 (dsSeCHY2). (A) Location of dsSeCHY2 in cDNA of SeCHY2. (B) Directional cloning of dsSeCHY2 into L4440 vector using two restriction sites of Hind III and Spe I. The cloning site was located between two opposite T7 promoters. Recombinant vector was screened after transformation into E . coli in the presence of ampicillin antibiotics. LacZ promoter (ʻlacZʼ) was then induced by IPTG to express T7 RNA polymerase (ʻT7 RPʼ) which recognized two T7 promoters and transcribed dsSeCHY2 in both directions. (C) Resulting dsSeCHY2 and its resistance to RNase A treatment. ʻWildʼ and ʻRCʼ represent non-recombinant and recombinant HT115 bacteria, respectively. ʻMʼ represents DNA marker.

    Article Snippet: For each reaction, 1 μL (4 U/μL) of RNase A inhibitor (QIAGEN, Hilden, Germany) was used.

    Techniques: Recombinant, Expressing, Clone Assay, Plasmid Preparation, Transformation Assay, Marker

    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and PCR Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown

    Journal: BMC Genomics

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application

    doi: 10.1186/s12864-017-4371-5

    Figure Lengend Snippet: DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and PCR Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown

    Article Snippet: After treatment with RNase A and proteinase K, DNA was purified by Qiagen MinElute PCR Purification Kit (Cat. # 28006, Valencia, CA) and quantified using Qubit dsDNA High Sensitivity assay (Invitrogen, Q32851).

    Techniques: DNA Purification, Chromatin Immunoprecipitation, Purification, Generated, Derivative Assay, Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction