rnase a Millipore Search Results


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  • 99
    Millipore disulfide scrambled rnase a
    Profile fitting to the hkl = −8 1 0 peak from <t>ribonuclease</t> A crystal using four asymmetric functions. ( a ) Gaussian convolved with two back-to-back exponentials fit. ( b ) Pseudo-Voigt function convolved with two back-to-back exponentials fit. ( c ) Gaussian convolved with Ikeda–Carpenter function fit. ( d ) Gaussian convolved with Landau function fit. In panels ( a–c ), SciPy was used to fit the functions and results were plotted by Gnuplot. In panel ( d ), because Gaussian convolved with Landau function contains convolution part in the equation, ROOT was used to fit the function and plot the results. Both four points of the outside regions of the integration region were used as the background region.
    Disulfide Scrambled Rnase A, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
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    99
    Millipore ribonuclease a rnase a
    The binding isotherm of <t>RNase</t> A and 3′-CMP. RNase A (55 μM) in 0.2 M sodium acetate buffer, pH 6.0 containing 0.2 M NaCl, was titrated with small injections of 3′-CMP solution in the same buffer. The enthalpy data were fitted to a single site binding model. ( A ) Binding isotherm for refolded RNase A; ( B ) binding isotherm for Sigma RNase A.
    Ribonuclease A Rnase A, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 360 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribonuclease a rnase a/product/Millipore
    Average 99 stars, based on 360 article reviews
    Price from $9.99 to $1999.99
    ribonuclease a rnase a - by Bioz Stars, 2020-07
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    99
    Millipore ribonuclease a rnasea
    Validation of RIP-seq data using qRT-PCR. MeCP2-RNA candidate interactions were validated in mouse primary cortical neurons using qRT PCR. (A) Data presented as percent of input (total chromatin RNA) bound by MeCP2 and normalized to the percent input bound in no antibody control samples. (B) MicroRNA miR-375 qRT PCR with and without RNase treatment. (C) MicroRNA miR-126 qRT PCR after <t>RNase</t> A treatment of MeCP2 bound RNAs in mouse primary cortical neurons. RNase A treatment of RNA isolated after MeCP2-RIP decreased amplification of miR-126 from both S1 and S2 chromatin fractions. (D) Let-7i qRT PCR after RNase A treatment of MeCP2 bound RNAs in mouse primary cortical neurons. RNase A treatment of RNA isolated after MeCP2-RIP decreased amplification of let-7i from both S1 and S2 chromatin fractions; S1, MNase sensitive chromatin fraction; S2, MNase resistant chromatin fraction; n = 3; t test * P
    Ribonuclease A Rnasea, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribonuclease a rnasea/product/Millipore
    Average 99 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    ribonuclease a rnasea - by Bioz Stars, 2020-07
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    94
    Millipore ribonuclease a rnase
    Validation of RIP-seq data using qRT-PCR. MeCP2-RNA candidate interactions were validated in mouse primary cortical neurons using qRT PCR. (A) Data presented as percent of input (total chromatin RNA) bound by MeCP2 and normalized to the percent input bound in no antibody control samples. (B) MicroRNA miR-375 qRT PCR with and without RNase treatment. (C) MicroRNA miR-126 qRT PCR after <t>RNase</t> A treatment of MeCP2 bound RNAs in mouse primary cortical neurons. RNase A treatment of RNA isolated after MeCP2-RIP decreased amplification of miR-126 from both S1 and S2 chromatin fractions. (D) Let-7i qRT PCR after RNase A treatment of MeCP2 bound RNAs in mouse primary cortical neurons. RNase A treatment of RNA isolated after MeCP2-RIP decreased amplification of let-7i from both S1 and S2 chromatin fractions; S1, MNase sensitive chromatin fraction; S2, MNase resistant chromatin fraction; n = 3; t test * P
    Ribonuclease A Rnase, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribonuclease a rnase/product/Millipore
    Average 94 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    Profile fitting to the hkl = −8 1 0 peak from ribonuclease A crystal using four asymmetric functions. ( a ) Gaussian convolved with two back-to-back exponentials fit. ( b ) Pseudo-Voigt function convolved with two back-to-back exponentials fit. ( c ) Gaussian convolved with Ikeda–Carpenter function fit. ( d ) Gaussian convolved with Landau function fit. In panels ( a–c ), SciPy was used to fit the functions and results were plotted by Gnuplot. In panel ( d ), because Gaussian convolved with Landau function contains convolution part in the equation, ROOT was used to fit the function and plot the results. Both four points of the outside regions of the integration region were used as the background region.

    Journal: Scientific Reports

    Article Title: Application of profile fitting method to neutron time-of-flight protein single crystal diffraction data collected at the iBIX

    doi: 10.1038/srep36628

    Figure Lengend Snippet: Profile fitting to the hkl = −8 1 0 peak from ribonuclease A crystal using four asymmetric functions. ( a ) Gaussian convolved with two back-to-back exponentials fit. ( b ) Pseudo-Voigt function convolved with two back-to-back exponentials fit. ( c ) Gaussian convolved with Ikeda–Carpenter function fit. ( d ) Gaussian convolved with Landau function fit. In panels ( a–c ), SciPy was used to fit the functions and results were plotted by Gnuplot. In panel ( d ), because Gaussian convolved with Landau function contains convolution part in the equation, ROOT was used to fit the function and plot the results. Both four points of the outside regions of the integration region were used as the background region.

    Article Snippet: Preparation of ribonuclease A crystal and neutron and X-ray diffraction experiments Bovine pancreatic ribonuclease A was purchased from Sigma-Aldrich and crystalized as previously described .

    Techniques:

    Plots of parameters related to peak profile against TOF in ribonuclease A neutron diffraction data. The peaks whose I/σ(I ) is over 5 and obtained by one detector located at 51° in 2 θ center and one crystal orientation were used. ( a ) Plot of parameter β against TOF. The peaks whose β errors are less than 1 were used. ( b ) Plot of parameter σ . The peaks whose σ errors are less than 10000 were used.

    Journal: Scientific Reports

    Article Title: Application of profile fitting method to neutron time-of-flight protein single crystal diffraction data collected at the iBIX

    doi: 10.1038/srep36628

    Figure Lengend Snippet: Plots of parameters related to peak profile against TOF in ribonuclease A neutron diffraction data. The peaks whose I/σ(I ) is over 5 and obtained by one detector located at 51° in 2 θ center and one crystal orientation were used. ( a ) Plot of parameter β against TOF. The peaks whose β errors are less than 1 were used. ( b ) Plot of parameter σ . The peaks whose σ errors are less than 10000 were used.

    Article Snippet: Preparation of ribonuclease A crystal and neutron and X-ray diffraction experiments Bovine pancreatic ribonuclease A was purchased from Sigma-Aldrich and crystalized as previously described .

    Techniques:

    Two examples of profile fitting to the weak peaks from ribonuclease A crystal using Gaussian convolved with two back-to-back exponentials. Red solid line: fitting function. Black points and line: weak peak profile. These peaks were obtained by detector located at 118° in 2 θ center and one crystal orientation. Both four points of outside regions of the integration region were used as the background region. ( a ) Peak hkl is 11 3 16. ( b ) Peak hkl is 11 3 20.

    Journal: Scientific Reports

    Article Title: Application of profile fitting method to neutron time-of-flight protein single crystal diffraction data collected at the iBIX

    doi: 10.1038/srep36628

    Figure Lengend Snippet: Two examples of profile fitting to the weak peaks from ribonuclease A crystal using Gaussian convolved with two back-to-back exponentials. Red solid line: fitting function. Black points and line: weak peak profile. These peaks were obtained by detector located at 118° in 2 θ center and one crystal orientation. Both four points of outside regions of the integration region were used as the background region. ( a ) Peak hkl is 11 3 16. ( b ) Peak hkl is 11 3 20.

    Article Snippet: Preparation of ribonuclease A crystal and neutron and X-ray diffraction experiments Bovine pancreatic ribonuclease A was purchased from Sigma-Aldrich and crystalized as previously described .

    Techniques:

    The binding isotherm of RNase A and 3′-CMP. RNase A (55 μM) in 0.2 M sodium acetate buffer, pH 6.0 containing 0.2 M NaCl, was titrated with small injections of 3′-CMP solution in the same buffer. The enthalpy data were fitted to a single site binding model. ( A ) Binding isotherm for refolded RNase A; ( B ) binding isotherm for Sigma RNase A.

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: Refolding and simultaneous purification by three-phase partitioning of recombinant proteins from inclusion bodies

    doi: 10.1110/ps.036939.108

    Figure Lengend Snippet: The binding isotherm of RNase A and 3′-CMP. RNase A (55 μM) in 0.2 M sodium acetate buffer, pH 6.0 containing 0.2 M NaCl, was titrated with small injections of 3′-CMP solution in the same buffer. The enthalpy data were fitted to a single site binding model. ( A ) Binding isotherm for refolded RNase A; ( B ) binding isotherm for Sigma RNase A.

    Article Snippet: The second interfacial precipitate (lane 4) shows a single band of RNase A having a purity comparable to that of commercially available RNase A (type XIIA) from Sigma Chemical Co. (lane 3).

    Techniques: Binding Assay

    Spectroscopic characterization of refolded RNase A. ( A ) Fluorescence emission spectra of RNase A. The samples at a concentration of 100 μg/mL −1 (∼7 μM) were excited at 278 nm and the emission spectra from 290 to 400 nm were recorded, using excitation and emission slit widths of 2 nm and 5 nm, respectively. Fluorescence spectra of unfolded RNase A (3), refolded RNase A (2), Sigma RNase A (1) are shown after correction for buffer contribution. ( B ) The far-UV CD spectra of refolded RNase A (○) and Sigma RNase A (△) for 0.5 mg/mL −1 protein recorded in a 1-mm path length cuvette from 200 to 250 nm. ( C ) The near-UV CD spectra of the two samples recorded for 0.5 mg/mL −1 protein in a 1-cm path length cuvette from 250 to 350 nm. The symbols are the same as in B .

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: Refolding and simultaneous purification by three-phase partitioning of recombinant proteins from inclusion bodies

    doi: 10.1110/ps.036939.108

    Figure Lengend Snippet: Spectroscopic characterization of refolded RNase A. ( A ) Fluorescence emission spectra of RNase A. The samples at a concentration of 100 μg/mL −1 (∼7 μM) were excited at 278 nm and the emission spectra from 290 to 400 nm were recorded, using excitation and emission slit widths of 2 nm and 5 nm, respectively. Fluorescence spectra of unfolded RNase A (3), refolded RNase A (2), Sigma RNase A (1) are shown after correction for buffer contribution. ( B ) The far-UV CD spectra of refolded RNase A (○) and Sigma RNase A (△) for 0.5 mg/mL −1 protein recorded in a 1-mm path length cuvette from 200 to 250 nm. ( C ) The near-UV CD spectra of the two samples recorded for 0.5 mg/mL −1 protein in a 1-cm path length cuvette from 250 to 350 nm. The symbols are the same as in B .

    Article Snippet: The second interfacial precipitate (lane 4) shows a single band of RNase A having a purity comparable to that of commercially available RNase A (type XIIA) from Sigma Chemical Co. (lane 3).

    Techniques: Fluorescence, Concentration Assay

    TPP purification monitored by SDS-PAGE. ( A ) Fifteen percent SDS-PAGE analysis of RNase A inclusion bodies at different steps of TPP; (lane 1 ) unwashed inclusion bodies; (lane 2 ) washed inclusion bodies with 50 mM PBS/pH 7.4/1 mM EDTA containing 2 M urea; (lane 3 ) commercial RNase A (Sigma Chemical Co.); (lane 4 ) refolded RNase A obtained from interfacial precipitate of second TPP; (lane 5 ) interfacial precipitate of first TPP; (lane 6 ) aqueous phase of second TPP. ( B ) Fifteen percent SDS-PAGE analysis of inclusion bodies of CcdB mutants subjected to TPP; (lane 1 ) unwashed inclusion bodies of CcdB-F17P; (lane 2 ) washed inclusion bodies of CcdB-F17P with 50 mM PBS/pH 7.4/0.5% Triton X-100; (lane 3 ) interfacial precipitate of first TPP; (lane 4 ) aqueous phase of second TPP; (lane 5 ) refolded and purified CcdB-F17P by TPP; (lane 6 ) molecular weight marker; (lane 7 ) unwashed inclusion bodies of CcdB-M97K; (lane 8 ) washed inclusion bodies of CcdB-M97K; (lane 9 ) interfacial precipitate of first TPP; (lane 10 ) aqueous phase of second TPP; (lane 11 ) refolded and purified CcdB-M97K by TPP. ( C ) Fifteen percent SDS-PAGE analysis of inclusion bodies of CD4D12 subjected to TPP CD4D12; (lane 1 ) molecular weight marker; (lane 2 ) unwashed inclusion bodies of CD4D12; (lane 3 ) washed inclusion bodies of CD4D12 with 50 mM PBS/pH 7.4/0.5% Triton X-100; (lane 4 ) interfacial precipitate of first TPP; (lane 5 ) aqueous phase of second TPP; (lane 6 ) refolded and purified CD4D12 by TPP; (lane 7 ) refolded and purified CD4D12 after subjected to the 50-kDa polyethersulfone membrane (PALL Lifesciences) once; (lane 8 ) refolded and purified CD4D12 after subjected to the 50-kDa polyethersulfone membrane (PALL Lifesciences) twice; (lane 9 ) refolded and purified CD4D12 in the absence of DTT.

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: Refolding and simultaneous purification by three-phase partitioning of recombinant proteins from inclusion bodies

    doi: 10.1110/ps.036939.108

    Figure Lengend Snippet: TPP purification monitored by SDS-PAGE. ( A ) Fifteen percent SDS-PAGE analysis of RNase A inclusion bodies at different steps of TPP; (lane 1 ) unwashed inclusion bodies; (lane 2 ) washed inclusion bodies with 50 mM PBS/pH 7.4/1 mM EDTA containing 2 M urea; (lane 3 ) commercial RNase A (Sigma Chemical Co.); (lane 4 ) refolded RNase A obtained from interfacial precipitate of second TPP; (lane 5 ) interfacial precipitate of first TPP; (lane 6 ) aqueous phase of second TPP. ( B ) Fifteen percent SDS-PAGE analysis of inclusion bodies of CcdB mutants subjected to TPP; (lane 1 ) unwashed inclusion bodies of CcdB-F17P; (lane 2 ) washed inclusion bodies of CcdB-F17P with 50 mM PBS/pH 7.4/0.5% Triton X-100; (lane 3 ) interfacial precipitate of first TPP; (lane 4 ) aqueous phase of second TPP; (lane 5 ) refolded and purified CcdB-F17P by TPP; (lane 6 ) molecular weight marker; (lane 7 ) unwashed inclusion bodies of CcdB-M97K; (lane 8 ) washed inclusion bodies of CcdB-M97K; (lane 9 ) interfacial precipitate of first TPP; (lane 10 ) aqueous phase of second TPP; (lane 11 ) refolded and purified CcdB-M97K by TPP. ( C ) Fifteen percent SDS-PAGE analysis of inclusion bodies of CD4D12 subjected to TPP CD4D12; (lane 1 ) molecular weight marker; (lane 2 ) unwashed inclusion bodies of CD4D12; (lane 3 ) washed inclusion bodies of CD4D12 with 50 mM PBS/pH 7.4/0.5% Triton X-100; (lane 4 ) interfacial precipitate of first TPP; (lane 5 ) aqueous phase of second TPP; (lane 6 ) refolded and purified CD4D12 by TPP; (lane 7 ) refolded and purified CD4D12 after subjected to the 50-kDa polyethersulfone membrane (PALL Lifesciences) once; (lane 8 ) refolded and purified CD4D12 after subjected to the 50-kDa polyethersulfone membrane (PALL Lifesciences) twice; (lane 9 ) refolded and purified CD4D12 in the absence of DTT.

    Article Snippet: The second interfacial precipitate (lane 4) shows a single band of RNase A having a purity comparable to that of commercially available RNase A (type XIIA) from Sigma Chemical Co. (lane 3).

    Techniques: Purification, SDS Page, Molecular Weight, Marker

    Structure determination of the anti-RNase A VHH#24/RNase A complex

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: A Combinatorial Histidine Scanning Library Approach to Engineer Highly pH-Dependent Protein Switches

    doi: 10.1002/pro.696

    Figure Lengend Snippet: Structure determination of the anti-RNase A VHH#24/RNase A complex

    Article Snippet: Bovine RNase A (Sigma-Aldrich) was biotinylated using EZ-Link Sulfo-NHS-SS-Biotin (Pierce, Rockford) according to the manufacturer's protocol (Promega, Madison WI).

    Techniques:

    The pH dependence of the observed binding constant,  K obs , for the consensus VHH#10 (A) and VHH#24 (B) variants. For reference, the wild-type VHH/RNase A binding data is presented, as well as simulated curves for a single ionizable group undergoing a range

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: A Combinatorial Histidine Scanning Library Approach to Engineer Highly pH-Dependent Protein Switches

    doi: 10.1002/pro.696

    Figure Lengend Snippet: The pH dependence of the observed binding constant, K obs , for the consensus VHH#10 (A) and VHH#24 (B) variants. For reference, the wild-type VHH/RNase A binding data is presented, as well as simulated curves for a single ionizable group undergoing a range

    Article Snippet: Bovine RNase A (Sigma-Aldrich) was biotinylated using EZ-Link Sulfo-NHS-SS-Biotin (Pierce, Rockford) according to the manufacturer's protocol (Promega, Madison WI).

    Techniques: Binding Assay

    (A) Amino acid sequences of pH sensitive VHH variants. (B) Top and (C) side views of the VHH interface side chain residues color coded by histidine hot-spot incorporation frequency. Color map provided in legend. RNase A-white sticks.

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: A Combinatorial Histidine Scanning Library Approach to Engineer Highly pH-Dependent Protein Switches

    doi: 10.1002/pro.696

    Figure Lengend Snippet: (A) Amino acid sequences of pH sensitive VHH variants. (B) Top and (C) side views of the VHH interface side chain residues color coded by histidine hot-spot incorporation frequency. Color map provided in legend. RNase A-white sticks.

    Article Snippet: Bovine RNase A (Sigma-Aldrich) was biotinylated using EZ-Link Sulfo-NHS-SS-Biotin (Pierce, Rockford) according to the manufacturer's protocol (Promega, Madison WI).

    Techniques:

    Validation of RIP-seq data using qRT-PCR. MeCP2-RNA candidate interactions were validated in mouse primary cortical neurons using qRT PCR. (A) Data presented as percent of input (total chromatin RNA) bound by MeCP2 and normalized to the percent input bound in no antibody control samples. (B) MicroRNA miR-375 qRT PCR with and without RNase treatment. (C) MicroRNA miR-126 qRT PCR after RNase A treatment of MeCP2 bound RNAs in mouse primary cortical neurons. RNase A treatment of RNA isolated after MeCP2-RIP decreased amplification of miR-126 from both S1 and S2 chromatin fractions. (D) Let-7i qRT PCR after RNase A treatment of MeCP2 bound RNAs in mouse primary cortical neurons. RNase A treatment of RNA isolated after MeCP2-RIP decreased amplification of let-7i from both S1 and S2 chromatin fractions; S1, MNase sensitive chromatin fraction; S2, MNase resistant chromatin fraction; n = 3; t test * P

    Journal: Epigenetics

    Article Title: MeCP2 interacts with chromosomal microRNAs in brain

    doi: 10.1080/15592294.2017.1391429

    Figure Lengend Snippet: Validation of RIP-seq data using qRT-PCR. MeCP2-RNA candidate interactions were validated in mouse primary cortical neurons using qRT PCR. (A) Data presented as percent of input (total chromatin RNA) bound by MeCP2 and normalized to the percent input bound in no antibody control samples. (B) MicroRNA miR-375 qRT PCR with and without RNase treatment. (C) MicroRNA miR-126 qRT PCR after RNase A treatment of MeCP2 bound RNAs in mouse primary cortical neurons. RNase A treatment of RNA isolated after MeCP2-RIP decreased amplification of miR-126 from both S1 and S2 chromatin fractions. (D) Let-7i qRT PCR after RNase A treatment of MeCP2 bound RNAs in mouse primary cortical neurons. RNase A treatment of RNA isolated after MeCP2-RIP decreased amplification of let-7i from both S1 and S2 chromatin fractions; S1, MNase sensitive chromatin fraction; S2, MNase resistant chromatin fraction; n = 3; t test * P

    Article Snippet: For RNase digestion, immunopurified RNA was digested with RNase A solution (Sigma, R4642) at a final concentration of 10 µg/ml and incubated at RT for 10 min prior to RNA purification.

    Techniques: Quantitative RT-PCR, Isolation, Amplification

    Example of TOF diffraction patterns of RNase A obtained by one detector located at 54° in 2θ.

    Journal: Journal of Synchrotron Radiation

    Article Title: Evaluation of performance for IBARAKI biological crystal diffractometer iBIX with new detectors

    doi: 10.1107/S0909049513021845

    Figure Lengend Snippet: Example of TOF diffraction patterns of RNase A obtained by one detector located at 54° in 2θ.

    Article Snippet: Bovine pancreatic ribonuclease A (type XII-A: R5500, Lot No. 055K7695) was purchased from Sigma-Aldrich.

    Techniques:

    Effect of CID pre-activation event on sequence coverage and observed product ion types for reduced and alkylated RNase A and RNase B. The coverage and number of different ion types obtained from pre-AIETD (CID)  and pre-AIECD (CID)  experiments at different

    Journal: Proteomics

    Article Title: Top-Down Tandem Mass Spectrometry on RNase A and B Using a Qh/FT-ICR Hybrid Mass Spectrometer

    doi: 10.1002/pmic.201300433

    Figure Lengend Snippet: Effect of CID pre-activation event on sequence coverage and observed product ion types for reduced and alkylated RNase A and RNase B. The coverage and number of different ion types obtained from pre-AIETD (CID) and pre-AIECD (CID) experiments at different

    Article Snippet: Bovine pancreas ribonuclease A and B (RNase A and B), dithiothreitol (DTT) and ammonium bicarbonate were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Activation Assay, Sequencing

    Sequence coverage obtained from CID (collision energy 8 eV, 40 scans), IRMPD (IR time 800 ms, 40 scans), ETD and ECD experiments for reduced (200 scans) (a) RNase A, (b) RNase B. Reproducible sequence coverage was achieved from triplicate analyses.

    Journal: Proteomics

    Article Title: Top-Down Tandem Mass Spectrometry on RNase A and B Using a Qh/FT-ICR Hybrid Mass Spectrometer

    doi: 10.1002/pmic.201300433

    Figure Lengend Snippet: Sequence coverage obtained from CID (collision energy 8 eV, 40 scans), IRMPD (IR time 800 ms, 40 scans), ETD and ECD experiments for reduced (200 scans) (a) RNase A, (b) RNase B. Reproducible sequence coverage was achieved from triplicate analyses.

    Article Snippet: Bovine pancreas ribonuclease A and B (RNase A and B), dithiothreitol (DTT) and ammonium bicarbonate were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Sequencing, Mass Spectrometry

    Sequence obtained from pre- or post-activation event after ETD or ECD (CID/IRMPD) AIETD (IRMPD-300ms)  for reduced and alkylated (a) RNase A and (b) RNase B. Grey highlights indicated complementary ions observed in these experiments, as compared to those

    Journal: Proteomics

    Article Title: Top-Down Tandem Mass Spectrometry on RNase A and B Using a Qh/FT-ICR Hybrid Mass Spectrometer

    doi: 10.1002/pmic.201300433

    Figure Lengend Snippet: Sequence obtained from pre- or post-activation event after ETD or ECD (CID/IRMPD) AIETD (IRMPD-300ms) for reduced and alkylated (a) RNase A and (b) RNase B. Grey highlights indicated complementary ions observed in these experiments, as compared to those

    Article Snippet: Bovine pancreas ribonuclease A and B (RNase A and B), dithiothreitol (DTT) and ammonium bicarbonate were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Sequencing, Activation Assay