Journal: PLoS ONE
Article Title: Free Extracellular miRNA Functionally Targets Cells by Transfecting Exosomes from Their Companion Cells
Figure Lengend Snippet: Enzymatic treatment of nucleic acids and associated proteins from PCE and QRNA of TNP Ts Sup eliminates suppressive activity. a . TNP Ts Sup-derived PCE suppression is sensitive to RNase A (Sigma 4375), but not DNase treatment (Group D vs C). b . Purer RNase A (Sigma 5250, Group E) and RNase III (Group H), as well as proteinase K with and without SDS (Groups F and G) treatment of QRNA from Sup of TNP Ts, eliminates its suppressive activity.
Article Snippet: Reagents Picryl chloride (PCl/ trinitrophenyl (TNP)-Cl), oxazolone (OX), EDTA sodium salt, 2-mercaptoethanol (2ME), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), trinitrobenzene sulfonic acid (TNBSA), cacodylic acid, sodium acetate, trypan blue, phenol-chloroform pH 4.7, phenol equilibrated with Tris-HCl buffer pH = 8.0, RNase A (4375 and purer 5250), DNase (D7231, Lot K1815), Proteinase K (P2308, Lot 65u8603), human recombinant IL-2, all were from [Sigma, St. Louis, MO].
Techniques: Activity Assay, Derivative Assay