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    Qiagen rnaprotect
    Growth and bile acid metabolism by Clostridium scindens ATCC 35704. (A) Growth profiles after a minimum of three sequential transfers under each culture condition: ♦, brain heart infusion (BHI) broth; ○, undefined medium (UM; DM with 0.1% yeast extract); Δ, defined medium (DM); ▲, PO 4 -buffered DM; ■, minimal medium (MM); and □, PO 4 -buffered MM. (B) Bile acid metabolism in DM and MM (left to right): lane 1, cholate (CA) standard; lane 2, deoxycholate (DCA) standard; lane 3, DM and C. scindens 35704; lane 4, DM with CA, and C. scindens 35704; lane 5, DM modified to contain tryptophan as the sole amino acid plus CA and C. scindens 35704; lane 6, DM modified to contain riboflavin, pantothenic acid, and pyridoxal HCl as sole vitamins plus CA and C. scindens 35704; and lane 7, MM with CA and C. scindens 35704. The initial concentration of CA and DCA in standards and CA in cultures was 100 µM. (C) Growth profiles in PO 4 -buffered DM in the absence (control, ▲) and presence of cholic acid (CA, □) or deoxycholic acid (DCA, ●). Arrows indicate addition of 50 μM bile acid, and the star indicates addition of <t>RNAprotect</t> for RNA-Seq analysis as well as removal of a 1-ml aliquot for the [24- 14 C]CA conversion assay. (D) Determination of the rate of conversion of [24- 14 C]CA to [24- 14 C]DCA. The inset is the autoradiograph of TLC-separated [24- 14 C]CA metabolites. CA and 7-oxo-DCA were scraped and counted, and DCA and 3-oxo-DCA were scraped and counted.
    Rnaprotect, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 23517 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnaprotect/product/Qiagen
    Average 99 stars, based on 23517 article reviews
    Price from $9.99 to $1999.99
    rnaprotect - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    91
    Becton Dickinson rnaprotect cell reagent
    Growth and bile acid metabolism by Clostridium scindens ATCC 35704. (A) Growth profiles after a minimum of three sequential transfers under each culture condition: ♦, brain heart infusion (BHI) broth; ○, undefined medium (UM; DM with 0.1% yeast extract); Δ, defined medium (DM); ▲, PO 4 -buffered DM; ■, minimal medium (MM); and □, PO 4 -buffered MM. (B) Bile acid metabolism in DM and MM (left to right): lane 1, cholate (CA) standard; lane 2, deoxycholate (DCA) standard; lane 3, DM and C. scindens 35704; lane 4, DM with CA, and C. scindens 35704; lane 5, DM modified to contain tryptophan as the sole amino acid plus CA and C. scindens 35704; lane 6, DM modified to contain riboflavin, pantothenic acid, and pyridoxal HCl as sole vitamins plus CA and C. scindens 35704; and lane 7, MM with CA and C. scindens 35704. The initial concentration of CA and DCA in standards and CA in cultures was 100 µM. (C) Growth profiles in PO 4 -buffered DM in the absence (control, ▲) and presence of cholic acid (CA, □) or deoxycholic acid (DCA, ●). Arrows indicate addition of 50 μM bile acid, and the star indicates addition of <t>RNAprotect</t> for RNA-Seq analysis as well as removal of a 1-ml aliquot for the [24- 14 C]CA conversion assay. (D) Determination of the rate of conversion of [24- 14 C]CA to [24- 14 C]DCA. The inset is the autoradiograph of TLC-separated [24- 14 C]CA metabolites. CA and 7-oxo-DCA were scraped and counted, and DCA and 3-oxo-DCA were scraped and counted.
    Rnaprotect Cell Reagent, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnaprotect cell reagent/product/Becton Dickinson
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rnaprotect cell reagent - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    Image Search Results


    Growth and bile acid metabolism by Clostridium scindens ATCC 35704. (A) Growth profiles after a minimum of three sequential transfers under each culture condition: ♦, brain heart infusion (BHI) broth; ○, undefined medium (UM; DM with 0.1% yeast extract); Δ, defined medium (DM); ▲, PO 4 -buffered DM; ■, minimal medium (MM); and □, PO 4 -buffered MM. (B) Bile acid metabolism in DM and MM (left to right): lane 1, cholate (CA) standard; lane 2, deoxycholate (DCA) standard; lane 3, DM and C. scindens 35704; lane 4, DM with CA, and C. scindens 35704; lane 5, DM modified to contain tryptophan as the sole amino acid plus CA and C. scindens 35704; lane 6, DM modified to contain riboflavin, pantothenic acid, and pyridoxal HCl as sole vitamins plus CA and C. scindens 35704; and lane 7, MM with CA and C. scindens 35704. The initial concentration of CA and DCA in standards and CA in cultures was 100 µM. (C) Growth profiles in PO 4 -buffered DM in the absence (control, ▲) and presence of cholic acid (CA, □) or deoxycholic acid (DCA, ●). Arrows indicate addition of 50 μM bile acid, and the star indicates addition of RNAprotect for RNA-Seq analysis as well as removal of a 1-ml aliquot for the [24- 14 C]CA conversion assay. (D) Determination of the rate of conversion of [24- 14 C]CA to [24- 14 C]DCA. The inset is the autoradiograph of TLC-separated [24- 14 C]CA metabolites. CA and 7-oxo-DCA were scraped and counted, and DCA and 3-oxo-DCA were scraped and counted.

    Journal: Applied and Environmental Microbiology

    Article Title: Clostridium scindens ATCC 35704: Integration of Nutritional Requirements, the Complete Genome Sequence, and Global Transcriptional Responses to Bile Acids

    doi: 10.1128/AEM.00052-19

    Figure Lengend Snippet: Growth and bile acid metabolism by Clostridium scindens ATCC 35704. (A) Growth profiles after a minimum of three sequential transfers under each culture condition: ♦, brain heart infusion (BHI) broth; ○, undefined medium (UM; DM with 0.1% yeast extract); Δ, defined medium (DM); ▲, PO 4 -buffered DM; ■, minimal medium (MM); and □, PO 4 -buffered MM. (B) Bile acid metabolism in DM and MM (left to right): lane 1, cholate (CA) standard; lane 2, deoxycholate (DCA) standard; lane 3, DM and C. scindens 35704; lane 4, DM with CA, and C. scindens 35704; lane 5, DM modified to contain tryptophan as the sole amino acid plus CA and C. scindens 35704; lane 6, DM modified to contain riboflavin, pantothenic acid, and pyridoxal HCl as sole vitamins plus CA and C. scindens 35704; and lane 7, MM with CA and C. scindens 35704. The initial concentration of CA and DCA in standards and CA in cultures was 100 µM. (C) Growth profiles in PO 4 -buffered DM in the absence (control, ▲) and presence of cholic acid (CA, □) or deoxycholic acid (DCA, ●). Arrows indicate addition of 50 μM bile acid, and the star indicates addition of RNAprotect for RNA-Seq analysis as well as removal of a 1-ml aliquot for the [24- 14 C]CA conversion assay. (D) Determination of the rate of conversion of [24- 14 C]CA to [24- 14 C]DCA. The inset is the autoradiograph of TLC-separated [24- 14 C]CA metabolites. CA and 7-oxo-DCA were scraped and counted, and DCA and 3-oxo-DCA were scraped and counted.

    Article Snippet: Cells were quenched with RNAprotect (Qiagen) and stored at −80°C until further processing.

    Techniques: Modification, Concentration Assay, RNA Sequencing Assay, Autoradiography, Thin Layer Chromatography

    Comparison of two blood sampling strategies for measuring gametocyte prevalence rates. (A) P. falciparum , (B) P. vivax . Gametocyte positivity (left panel) and transcript copy numbers (right panel) are shown for RNAprotect solution versus filter paper soaked in TRIzol. Only samples were compared for which both measurements were available.

    Journal: PLoS ONE

    Article Title: Strategies for Detection of Plasmodium species Gametocytes

    doi: 10.1371/journal.pone.0076316

    Figure Lengend Snippet: Comparison of two blood sampling strategies for measuring gametocyte prevalence rates. (A) P. falciparum , (B) P. vivax . Gametocyte positivity (left panel) and transcript copy numbers (right panel) are shown for RNAprotect solution versus filter paper soaked in TRIzol. Only samples were compared for which both measurements were available.

    Article Snippet: Substantial costs for the recommended RNA processing buffer and our RNA yields severely compromised by the necessity for DNase digestion of extracted RNA prompted us to discontinue this approach. (iii) RNAprotect® cell reagent: RNA was extracted from 300 µl total volume (50µl whole blood plus 250µl RNAprotect reagent) using the RNeasy® plus mini kit protocol for spin column followed by on-column DNase digestion (all from Qiagen).

    Techniques: Sampling

    NAM effectively reduces production of complement factors (A) Altered expression of several genes in the complement pathway detected by RNA-seq analysis of NAM treatment (n=7 donors; 4 AMD and 3 control; 7 lines). ; each sample is color matched across NAM and vehicle treatment. (C) ELISA measurement of secretion of C3 into the culture supernatant 60–72 hours after the last medium change in vehicle, 10mM NAM and C3shRNA treated hiPSC-RPE. Data are expressed as mean± SEM of absorbance from ELISA assay (n=3 donors; 2 AMD and 1 control; 4 lines). (D) qPCR analysis of AMD/drusen associated protein transcripts in C3shRNA and 10mM NAM treated hiPSC-RPE relative to vehicle (baseline defined as 100%). Data are expressed as mean± SEM (n=3 donors; 2 AMD and 1 control; 4 lines). (E) ELISA measurement of secretion of VEGF-A and APOJ into the culture supernatant 60–72 hours after the last medium change in vehicle, 10mM NAM and C3shRNA treated hiPSC-RPE. Data are expressed as mean± SEM of absorbance from ELISA assay (n=3 donors; 2 AMD and 1 control; 4 lines). (F) qPCR analysis of complement and inflammation associated protein transcripts in C3shRNA and 10mM NAM treated hiPSC-RPE relative to vehicle (baseline defined as 100%). Data are expressed as mean± SEM (n=3 donors; 2 AMD and 1 control; 4 lines). (G) LDH release into the culture supernatant of vehicle, 10mM NAM and C3shRNA treated hiPSC-RPE. Data are expressed as mean± SEM (n=3 donors; 2 AMD and 1 control; 4 lines). (H) qPCR analysis of TP53 and RPE genes expression in C3shRNA and 10mM NAM treated hiPSC-RPE relative to vehicle (baseline defined as 100%). Data are expressed as mean± SEM (n=3 donors; 2 AMD and 1 control; 4 lines). Paired Student’s t test (two-tailed) was used for statistical analysis. Paired Student’s t test (one-tailed) was used for statistical analysis, unless stated otherwise above (*= p

    Journal: Cell stem cell

    Article Title: Nicotinamide Ameliorates Disease Phenotypes in a Human iPSC Model of Age-related Macular Degeneration

    doi: 10.1016/j.stem.2016.12.015

    Figure Lengend Snippet: NAM effectively reduces production of complement factors (A) Altered expression of several genes in the complement pathway detected by RNA-seq analysis of NAM treatment (n=7 donors; 4 AMD and 3 control; 7 lines). ; each sample is color matched across NAM and vehicle treatment. (C) ELISA measurement of secretion of C3 into the culture supernatant 60–72 hours after the last medium change in vehicle, 10mM NAM and C3shRNA treated hiPSC-RPE. Data are expressed as mean± SEM of absorbance from ELISA assay (n=3 donors; 2 AMD and 1 control; 4 lines). (D) qPCR analysis of AMD/drusen associated protein transcripts in C3shRNA and 10mM NAM treated hiPSC-RPE relative to vehicle (baseline defined as 100%). Data are expressed as mean± SEM (n=3 donors; 2 AMD and 1 control; 4 lines). (E) ELISA measurement of secretion of VEGF-A and APOJ into the culture supernatant 60–72 hours after the last medium change in vehicle, 10mM NAM and C3shRNA treated hiPSC-RPE. Data are expressed as mean± SEM of absorbance from ELISA assay (n=3 donors; 2 AMD and 1 control; 4 lines). (F) qPCR analysis of complement and inflammation associated protein transcripts in C3shRNA and 10mM NAM treated hiPSC-RPE relative to vehicle (baseline defined as 100%). Data are expressed as mean± SEM (n=3 donors; 2 AMD and 1 control; 4 lines). (G) LDH release into the culture supernatant of vehicle, 10mM NAM and C3shRNA treated hiPSC-RPE. Data are expressed as mean± SEM (n=3 donors; 2 AMD and 1 control; 4 lines). (H) qPCR analysis of TP53 and RPE genes expression in C3shRNA and 10mM NAM treated hiPSC-RPE relative to vehicle (baseline defined as 100%). Data are expressed as mean± SEM (n=3 donors; 2 AMD and 1 control; 4 lines). Paired Student’s t test (two-tailed) was used for statistical analysis. Paired Student’s t test (one-tailed) was used for statistical analysis, unless stated otherwise above (*= p

    Article Snippet: To extract RNA, hiPSC-RPE cells were scraped from the culture wells and collected in RNA protect (Cat. # 76526; Qiagen).

    Techniques: Expressing, RNA Sequencing Assay, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Two Tailed Test, One-tailed Test

    RNA-seq analysis of action of NAM on hiPSC-RPE (A) Gene interaction network (confidence level=0.9) of the differentially expressed genes in NAM treated hiPSC-RPE compared to vehicle from RNA-seq analysis, using the STRING database (n=7 donors; 4 AMD and 3 control; 7 lines). Subnetworks (Neighborhoods) are colored and annotated with enriched functional categories. Gray lines: connections within a neighborhood; Red lines: connections between neighborhoods; Squares: Upregulated genes; Circles: Downregulated genes. (B-C) GO enrichment for KEGG pathways (B) and disease associations (C) of the differentially expressed genes (n=7 donors; 4 AMD and 3 control; 7 lines). .

    Journal: Cell stem cell

    Article Title: Nicotinamide Ameliorates Disease Phenotypes in a Human iPSC Model of Age-related Macular Degeneration

    doi: 10.1016/j.stem.2016.12.015

    Figure Lengend Snippet: RNA-seq analysis of action of NAM on hiPSC-RPE (A) Gene interaction network (confidence level=0.9) of the differentially expressed genes in NAM treated hiPSC-RPE compared to vehicle from RNA-seq analysis, using the STRING database (n=7 donors; 4 AMD and 3 control; 7 lines). Subnetworks (Neighborhoods) are colored and annotated with enriched functional categories. Gray lines: connections within a neighborhood; Red lines: connections between neighborhoods; Squares: Upregulated genes; Circles: Downregulated genes. (B-C) GO enrichment for KEGG pathways (B) and disease associations (C) of the differentially expressed genes (n=7 donors; 4 AMD and 3 control; 7 lines). .

    Article Snippet: To extract RNA, hiPSC-RPE cells were scraped from the culture wells and collected in RNA protect (Cat. # 76526; Qiagen).

    Techniques: RNA Sequencing Assay, Functional Assay

    URT cells express ALDH1A mRNA. RNA was extracted from cells and cDNA was synthesized using oligo-dT 20 primers. Serial 1∶10 dilutions of the cDNA were used for PCR amplifications. Gels were loaded from left to right with PCR products from serially diluted cDNA. The left-most columns were representative of products from ∼1×10 3 cells. Panel A. Results are shown for NT cells (see Materials and Methods), cervical lymph nodes (CLN) and mesenteric lymph nodes (MesLN) of naïve C57BL/6 mice, separated into CD11c Hi and CD11c Lo/neg populations and tested for ALDH1A2 and GAPDH mRNA. Panel B. CD11c Hi and CD11c Lo/neg NT populations were tested for ALDH1A1, ALDH1A2, ALDH1A3 and GAPDH mRNA. Panel C. Cells were tested for ALDH1A2 mRNA. Samples included NT cells that had been FACS-sorted for the F4/80 + CD11c - CD11b + phenotype (abbreviated ‘F4/80+CD11c-’), two macrophage lines MAC INF4.29 and LIE 13–14, NT cells or lung cells enriched for epithelium by negative selection and short-term culture (see Materials and Methods), and LET cells. On a per-cell basis, the highest ALDH1A expression levels were among CD11c Lo/neg cell populations.

    Journal: PLoS ONE

    Article Title: Respiratory Tract Epithelial Cells Express Retinaldehyde Dehydrogenase ALDH1A and Enhance IgA Production by Stimulated B Cells in the Presence of Vitamin A

    doi: 10.1371/journal.pone.0086554

    Figure Lengend Snippet: URT cells express ALDH1A mRNA. RNA was extracted from cells and cDNA was synthesized using oligo-dT 20 primers. Serial 1∶10 dilutions of the cDNA were used for PCR amplifications. Gels were loaded from left to right with PCR products from serially diluted cDNA. The left-most columns were representative of products from ∼1×10 3 cells. Panel A. Results are shown for NT cells (see Materials and Methods), cervical lymph nodes (CLN) and mesenteric lymph nodes (MesLN) of naïve C57BL/6 mice, separated into CD11c Hi and CD11c Lo/neg populations and tested for ALDH1A2 and GAPDH mRNA. Panel B. CD11c Hi and CD11c Lo/neg NT populations were tested for ALDH1A1, ALDH1A2, ALDH1A3 and GAPDH mRNA. Panel C. Cells were tested for ALDH1A2 mRNA. Samples included NT cells that had been FACS-sorted for the F4/80 + CD11c - CD11b + phenotype (abbreviated ‘F4/80+CD11c-’), two macrophage lines MAC INF4.29 and LIE 13–14, NT cells or lung cells enriched for epithelium by negative selection and short-term culture (see Materials and Methods), and LET cells. On a per-cell basis, the highest ALDH1A expression levels were among CD11c Lo/neg cell populations.

    Article Snippet: PCR Analysis for ALDH1A mRNA Cells were stored in RNA Protect Cell Reagent (Qiagen, Cat#76526) at −20°C and RNA was extracted from 1×105 cells using RNeasy Plus Mini Kits (Qiagen, Cat# 74134).

    Techniques: Synthesized, Polymerase Chain Reaction, Mouse Assay, FACS, Selection, Expressing