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  • 99
    New England Biolabs e coli rnap core enzyme
    Phi14:2 <t>RNAP</t> <t>gp66</t> is related to single- and multi-subunit RNAPs. a, b , and c , Crystal structures of phi14:2 gp66, QDE-1 from N. crassa (PDB 2J7N 6 ), and T. thermophilus RNAP (PDB ID 2O5J 17 ) are shown as ribbon diagrams, respectively. Conserved structural elements are colored according to the color code given above the top panels. Dissimilar domains of QDE-1 and gp66 are shown in gray color. Each of the five subunits comprising the T. thermophilus RNAP (αI, αII, β, β′, and ω) is rendered in a distinct color.
    E Coli Rnap Core Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli rnap core enzyme/product/New England Biolabs
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    88
    Pasteur Institute core rnap
    Phi14:2 <t>RNAP</t> <t>gp66</t> is related to single- and multi-subunit RNAPs. a, b , and c , Crystal structures of phi14:2 gp66, QDE-1 from N. crassa (PDB 2J7N 6 ), and T. thermophilus RNAP (PDB ID 2O5J 17 ) are shown as ribbon diagrams, respectively. Conserved structural elements are colored according to the color code given above the top panels. Dissimilar domains of QDE-1 and gp66 are shown in gray color. Each of the five subunits comprising the T. thermophilus RNAP (αI, αII, β, β′, and ω) is rendered in a distinct color.
    Core Rnap, supplied by Pasteur Institute, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/core rnap/product/Pasteur Institute
    Average 88 stars, based on 2 article reviews
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    core rnap - by Bioz Stars, 2020-09
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    85
    Epicentre Biotechnologies escherichia coli core rnap
    Phi14:2 <t>RNAP</t> <t>gp66</t> is related to single- and multi-subunit RNAPs. a, b , and c , Crystal structures of phi14:2 gp66, QDE-1 from N. crassa (PDB 2J7N 6 ), and T. thermophilus RNAP (PDB ID 2O5J 17 ) are shown as ribbon diagrams, respectively. Conserved structural elements are colored according to the color code given above the top panels. Dissimilar domains of QDE-1 and gp66 are shown in gray color. Each of the five subunits comprising the T. thermophilus RNAP (αI, αII, β, β′, and ω) is rendered in a distinct color.
    Escherichia Coli Core Rnap, supplied by Epicentre Biotechnologies, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli core rnap/product/Epicentre Biotechnologies
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    Price from $9.99 to $1999.99
    escherichia coli core rnap - by Bioz Stars, 2020-09
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    85
    Epicentre Technologies Corp escherichia coli core rnap
    Phi14:2 <t>RNAP</t> <t>gp66</t> is related to single- and multi-subunit RNAPs. a, b , and c , Crystal structures of phi14:2 gp66, QDE-1 from N. crassa (PDB 2J7N 6 ), and T. thermophilus RNAP (PDB ID 2O5J 17 ) are shown as ribbon diagrams, respectively. Conserved structural elements are colored according to the color code given above the top panels. Dissimilar domains of QDE-1 and gp66 are shown in gray color. Each of the five subunits comprising the T. thermophilus RNAP (αI, αII, β, β′, and ω) is rendered in a distinct color.
    Escherichia Coli Core Rnap, supplied by Epicentre Technologies Corp, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli core rnap/product/Epicentre Technologies Corp
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    escherichia coli core rnap - by Bioz Stars, 2020-09
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    88
    Epicentre Biotechnologies e coli rnap core enzyme
    Phi14:2 <t>RNAP</t> <t>gp66</t> is related to single- and multi-subunit RNAPs. a, b , and c , Crystal structures of phi14:2 gp66, QDE-1 from N. crassa (PDB 2J7N 6 ), and T. thermophilus RNAP (PDB ID 2O5J 17 ) are shown as ribbon diagrams, respectively. Conserved structural elements are colored according to the color code given above the top panels. Dissimilar domains of QDE-1 and gp66 are shown in gray color. Each of the five subunits comprising the T. thermophilus RNAP (αI, αII, β, β′, and ω) is rendered in a distinct color.
    E Coli Rnap Core Enzyme, supplied by Epicentre Biotechnologies, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli rnap core enzyme/product/Epicentre Biotechnologies
    Average 88 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    e coli rnap core enzyme - by Bioz Stars, 2020-09
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    88
    Epicentre Biotechnologies rnap core
    Phi14:2 <t>RNAP</t> <t>gp66</t> is related to single- and multi-subunit RNAPs. a, b , and c , Crystal structures of phi14:2 gp66, QDE-1 from N. crassa (PDB 2J7N 6 ), and T. thermophilus RNAP (PDB ID 2O5J 17 ) are shown as ribbon diagrams, respectively. Conserved structural elements are colored according to the color code given above the top panels. Dissimilar domains of QDE-1 and gp66 are shown in gray color. Each of the five subunits comprising the T. thermophilus RNAP (αI, αII, β, β′, and ω) is rendered in a distinct color.
    Rnap Core, supplied by Epicentre Biotechnologies, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnap core/product/Epicentre Biotechnologies
    Average 88 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    rnap core - by Bioz Stars, 2020-09
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    Image Search Results


    Phi14:2 RNAP gp66 is related to single- and multi-subunit RNAPs. a, b , and c , Crystal structures of phi14:2 gp66, QDE-1 from N. crassa (PDB 2J7N 6 ), and T. thermophilus RNAP (PDB ID 2O5J 17 ) are shown as ribbon diagrams, respectively. Conserved structural elements are colored according to the color code given above the top panels. Dissimilar domains of QDE-1 and gp66 are shown in gray color. Each of the five subunits comprising the T. thermophilus RNAP (αI, αII, β, β′, and ω) is rendered in a distinct color.

    Journal: bioRxiv

    Article Title: Structure and function of virion RNA polymerase of crAss-like phage

    doi: 10.1101/2020.03.07.982082

    Figure Lengend Snippet: Phi14:2 RNAP gp66 is related to single- and multi-subunit RNAPs. a, b , and c , Crystal structures of phi14:2 gp66, QDE-1 from N. crassa (PDB 2J7N 6 ), and T. thermophilus RNAP (PDB ID 2O5J 17 ) are shown as ribbon diagrams, respectively. Conserved structural elements are colored according to the color code given above the top panels. Dissimilar domains of QDE-1 and gp66 are shown in gray color. Each of the five subunits comprising the T. thermophilus RNAP (αI, αII, β, β′, and ω) is rendered in a distinct color.

    Article Snippet: Transcription reactions from RNA-DNA scaffolds were set at the same buffer as above transcription reactions and contained 15 nM RNA-DNA scaffold and 15 nM of gp66, T7 RNAP or E. coli RNAP core (New England Biolabs).

    Techniques:

    Cleft-blocking domain occupies the RNA-DNA hybrid binding site in phi14:2 RNAP gp66. a, b , and c , The structure of the active site of QDE-1 from N. crassa (PDB 2J7N 6 ), phi14:2 gp66, and T. thermophilus RNAP (PDB ID 2O5J 17 ), respectively. The active site of phi14:2 RNAP gp66 ( b ) is in a conformation incompatible with Mg binding.

    Journal: bioRxiv

    Article Title: Structure and function of virion RNA polymerase of crAss-like phage

    doi: 10.1101/2020.03.07.982082

    Figure Lengend Snippet: Cleft-blocking domain occupies the RNA-DNA hybrid binding site in phi14:2 RNAP gp66. a, b , and c , The structure of the active site of QDE-1 from N. crassa (PDB 2J7N 6 ), phi14:2 gp66, and T. thermophilus RNAP (PDB ID 2O5J 17 ), respectively. The active site of phi14:2 RNAP gp66 ( b ) is in a conformation incompatible with Mg binding.

    Article Snippet: Transcription reactions from RNA-DNA scaffolds were set at the same buffer as above transcription reactions and contained 15 nM RNA-DNA scaffold and 15 nM of gp66, T7 RNAP or E. coli RNAP core (New England Biolabs).

    Techniques: Blocking Assay, Binding Assay

    Identification of putative promoters in phi14:2 genome. a , Schematic of the phi14:2 genome (see the legend of Fig. 2a for details) with putative promoters marked by black arrows. b , Primer extension and sequencing reactions for eleven putative promoters (Supplementary Table 4). Major primer extension products are marked with black asterisks. c , Sequences flanking the primer extension endpoints are shown; nucleotides, corresponding to primer extension endpoints are colored red. Conserved nucleotides of putative middle promoters are shown in violet. d , Left panel: In vitro transcription of PCR-fragments containing the T7 A1 promoter and predicted phi14:2 P070 and P075 promoters by E. coli RNAP; Right panel: Primer extension reactions of RNA synthesized in vitro by E. coli RNAP from PCR-fragments containing phi14:2 P070 and P075 promoters.

    Journal: bioRxiv

    Article Title: Structure and function of virion RNA polymerase of crAss-like phage

    doi: 10.1101/2020.03.07.982082

    Figure Lengend Snippet: Identification of putative promoters in phi14:2 genome. a , Schematic of the phi14:2 genome (see the legend of Fig. 2a for details) with putative promoters marked by black arrows. b , Primer extension and sequencing reactions for eleven putative promoters (Supplementary Table 4). Major primer extension products are marked with black asterisks. c , Sequences flanking the primer extension endpoints are shown; nucleotides, corresponding to primer extension endpoints are colored red. Conserved nucleotides of putative middle promoters are shown in violet. d , Left panel: In vitro transcription of PCR-fragments containing the T7 A1 promoter and predicted phi14:2 P070 and P075 promoters by E. coli RNAP; Right panel: Primer extension reactions of RNA synthesized in vitro by E. coli RNAP from PCR-fragments containing phi14:2 P070 and P075 promoters.

    Article Snippet: Transcription reactions from RNA-DNA scaffolds were set at the same buffer as above transcription reactions and contained 15 nM RNA-DNA scaffold and 15 nM of gp66, T7 RNAP or E. coli RNAP core (New England Biolabs).

    Techniques: Sequencing, In Vitro, Polymerase Chain Reaction, Synthesized

    Global analysis of phi14:2 transcription during phi14:2 infection. a , Schematics of the phi14:2 genome 3 . ORFs are marked as arrows and numbered according to the study by Yutin et al 2 (Supplementary Table 1). Intergenic regions larger than 50 base pairs are shown as grey rectangles. Replicative, gene expression, and capsid gene modules are marked by green, violet, and blue dashed frames, correspondingly 2 . Early, middle, and late genes are colored green, purple, and blue, correspondingly. Heat maps indicating the temporal pattern of phi14:2 transcripts and relative abundance of phi14:2 transcripts are shown below the genome. In the top heat map, the transcript abundance for each gene or intergenic region longer than 50 base pairs is normalized to the maximum transcript abundance for this particular gene/intergenic region; In the bottom heat map, the same quantities were normalized to the absolute maximum that corresponded to the abundance of the late gene g091 (major capsid protein) at 190 min post infection. b , Time courses of accumulation of individual phi14:2 transcripts divided into three temporal classes during infection; the y axis shows abundance of individual genes transcripts normalized to the maximal value for this gene obtained in Rif-libraries. c , Transcription by gp66 of denatured phi14:2 DNA and by C. baltica RNAP of a PCR-fragment containing the T7 A1 promoter in the absence and in the presence of rifampicin. d , Time courses of accumulation of early, middle, and late phi14:2 transcripts during infection in the presence of rifampicin; the y axis shows abundance of individual genes transcripts in Rif+ libraries normalized to maximal value for this gene obtained in Rif-libraries. e , WebLogos of phi14:2 middle promoters located upstream of middle genes g070, g075, g108, and g110 (top panel) and cumulative consensus of 126 crAss-like phage middle/late promoters (bottom panel, Supplementary file 1).

    Journal: bioRxiv

    Article Title: Structure and function of virion RNA polymerase of crAss-like phage

    doi: 10.1101/2020.03.07.982082

    Figure Lengend Snippet: Global analysis of phi14:2 transcription during phi14:2 infection. a , Schematics of the phi14:2 genome 3 . ORFs are marked as arrows and numbered according to the study by Yutin et al 2 (Supplementary Table 1). Intergenic regions larger than 50 base pairs are shown as grey rectangles. Replicative, gene expression, and capsid gene modules are marked by green, violet, and blue dashed frames, correspondingly 2 . Early, middle, and late genes are colored green, purple, and blue, correspondingly. Heat maps indicating the temporal pattern of phi14:2 transcripts and relative abundance of phi14:2 transcripts are shown below the genome. In the top heat map, the transcript abundance for each gene or intergenic region longer than 50 base pairs is normalized to the maximum transcript abundance for this particular gene/intergenic region; In the bottom heat map, the same quantities were normalized to the absolute maximum that corresponded to the abundance of the late gene g091 (major capsid protein) at 190 min post infection. b , Time courses of accumulation of individual phi14:2 transcripts divided into three temporal classes during infection; the y axis shows abundance of individual genes transcripts normalized to the maximal value for this gene obtained in Rif-libraries. c , Transcription by gp66 of denatured phi14:2 DNA and by C. baltica RNAP of a PCR-fragment containing the T7 A1 promoter in the absence and in the presence of rifampicin. d , Time courses of accumulation of early, middle, and late phi14:2 transcripts during infection in the presence of rifampicin; the y axis shows abundance of individual genes transcripts in Rif+ libraries normalized to maximal value for this gene obtained in Rif-libraries. e , WebLogos of phi14:2 middle promoters located upstream of middle genes g070, g075, g108, and g110 (top panel) and cumulative consensus of 126 crAss-like phage middle/late promoters (bottom panel, Supplementary file 1).

    Article Snippet: Transcription reactions from RNA-DNA scaffolds were set at the same buffer as above transcription reactions and contained 15 nM RNA-DNA scaffold and 15 nM of gp66, T7 RNAP or E. coli RNAP core (New England Biolabs).

    Techniques: Infection, Expressing, Polymerase Chain Reaction

    In vitro transcription activity of the phi14:2 RNAP gp66. a , Transcription by gp66 of genomic DNA of phages phi14:2 and M13 (double- and single-stranded forms) at 30, 22, and 10°C; the reaction products were resolved by electrophoresis in 5 % (w/v) denaturing 8 M urea polyacrylamide gel and revealed by autoradiography. b , Transcription by gp66 of native and denatured genomic DNA of phage phi14:2. c , Completed transcription reactions of phi14:2 genomic DNA were treated with DNase RQ1 or RNase T1 prior to loading on the gel. d , Activity of gp66 requires Mg ions and ATP. Denatured genomic DNA of phi14:2 phage has been used as a template. e , Transcription of phi14:2 denatured genomic DNA by wild-type gp66 and gp66 mutants carrying single alanine substitutions of each aspartate in the DFDID motif.

    Journal: bioRxiv

    Article Title: Structure and function of virion RNA polymerase of crAss-like phage

    doi: 10.1101/2020.03.07.982082

    Figure Lengend Snippet: In vitro transcription activity of the phi14:2 RNAP gp66. a , Transcription by gp66 of genomic DNA of phages phi14:2 and M13 (double- and single-stranded forms) at 30, 22, and 10°C; the reaction products were resolved by electrophoresis in 5 % (w/v) denaturing 8 M urea polyacrylamide gel and revealed by autoradiography. b , Transcription by gp66 of native and denatured genomic DNA of phage phi14:2. c , Completed transcription reactions of phi14:2 genomic DNA were treated with DNase RQ1 or RNase T1 prior to loading on the gel. d , Activity of gp66 requires Mg ions and ATP. Denatured genomic DNA of phi14:2 phage has been used as a template. e , Transcription of phi14:2 denatured genomic DNA by wild-type gp66 and gp66 mutants carrying single alanine substitutions of each aspartate in the DFDID motif.

    Article Snippet: Transcription reactions from RNA-DNA scaffolds were set at the same buffer as above transcription reactions and contained 15 nM RNA-DNA scaffold and 15 nM of gp66, T7 RNAP or E. coli RNAP core (New England Biolabs).

    Techniques: In Vitro, Activity Assay, Electrophoresis, Autoradiography