rnai mediated knockdown Search Results


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  • 99
    Thermo Fisher silencer select negative control no 1 sirna
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    SLIT2 LTD rnai mediated knockdown
    Effects of silencing <t>SLIT2</t> on SRGAP1 expression and the GTPase activity of CDC42 and RAC1 in GCs. The granulosa cells were transfected with specific siRNAs targeting the SLIT2 gene, scrambled siRNA (negative control) or no siRNA (blank control). ( A ) The expression of the SRGAP1 gene before and after the GCs were transfected with the specific siRNA for 48 h was examined by qRT-PCR. The mRNA expression was normalized to that of the 18S rRNA gene; the values on the bar graphs represent the mean ± SEM of 10 hens (n = 10) from a representative experiment. ( B ) The expression levels of the SRGAP1 protein in the GCs with and without specific siRNA interference <t>(RNAi)</t> was detected by western blotting. β-actin was used as a loading control. ( C ) The immunoprecipitation (IP) with the PBD antibody was revealed by immunoblotting (IB) using monoclonal anti-CDC42 or anti-RAC1 antibodies (in the right-hand column). −, negative control; +, SLIT2 silencing group. All blots were cropped, and the gels were run under the same experimental conditions. ( D ) The expression levels of GTP-bound CDC42 and RAC1 under SLIT2 silencing were determined by western blotting. β-actin was used as a loading control. For each group, the different superscript above the bar indicates that the difference was significant (P
    Rnai Mediated Knockdown, supplied by SLIT2 LTD, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Syntaxin rnai mediated knockdown
    Knockdown of Vti1a, <t>syntaxin</t> 6, and Vti1b by <t>RNAi.</t> HeLa cells were treated with a pool of siRNAs for Vti1a ( A–G ), syntaxin 6 ( A and H–M ), or Vti1b ( A and N–S ), or control siRNAs ( A and T–Y ), as described under “Experimental
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    GE Healthcare rnai mediated knockdown rnai reagents
    Knockdown of Vti1a, <t>syntaxin</t> 6, and Vti1b by <t>RNAi.</t> HeLa cells were treated with a pool of siRNAs for Vti1a ( A–G ), syntaxin 6 ( A and H–M ), or Vti1b ( A and N–S ), or control siRNAs ( A and T–Y ), as described under “Experimental
    Rnai Mediated Knockdown Rnai Reagents, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore rna interference mediated knockdown
    Knockdown of Vti1a, <t>syntaxin</t> 6, and Vti1b by <t>RNAi.</t> HeLa cells were treated with a pool of siRNAs for Vti1a ( A–G ), syntaxin 6 ( A and H–M ), or Vti1b ( A and N–S ), or control siRNAs ( A and T–Y ), as described under “Experimental
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    Horizon Discovery rnai mediated knockdowns
    Knockdown of Vti1a, <t>syntaxin</t> 6, and Vti1b by <t>RNAi.</t> HeLa cells were treated with a pool of siRNAs for Vti1a ( A–G ), syntaxin 6 ( A and H–M ), or Vti1b ( A and N–S ), or control siRNAs ( A and T–Y ), as described under “Experimental
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    Shanghai Genechem shrna mediated fgfr2 knockdown lentivirus fgfr2 rnai vector
    Knockdown of Vti1a, <t>syntaxin</t> 6, and Vti1b by <t>RNAi.</t> HeLa cells were treated with a pool of siRNAs for Vti1a ( A–G ), syntaxin 6 ( A and H–M ), or Vti1b ( A and N–S ), or control siRNAs ( A and T–Y ), as described under “Experimental
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    Thermo Fisher sirna mediated knockdown experiments lipofectamine rnai max
    Knockdown of Vti1a, <t>syntaxin</t> 6, and Vti1b by <t>RNAi.</t> HeLa cells were treated with a pool of siRNAs for Vti1a ( A–G ), syntaxin 6 ( A and H–M ), or Vti1b ( A and N–S ), or control siRNAs ( A and T–Y ), as described under “Experimental
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    Shanghai Genechem short hairpin rnas shrnas mediated arid1a knockdown lentivirus arid1a rnai vector
    Knockdown of Vti1a, <t>syntaxin</t> 6, and Vti1b by <t>RNAi.</t> HeLa cells were treated with a pool of siRNAs for Vti1a ( A–G ), syntaxin 6 ( A and H–M ), or Vti1b ( A and N–S ), or control siRNAs ( A and T–Y ), as described under “Experimental
    Short Hairpin Rnas Shrnas Mediated Arid1a Knockdown Lentivirus Arid1a Rnai Vector, supplied by Shanghai Genechem, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effects of silencing SLIT2 on SRGAP1 expression and the GTPase activity of CDC42 and RAC1 in GCs. The granulosa cells were transfected with specific siRNAs targeting the SLIT2 gene, scrambled siRNA (negative control) or no siRNA (blank control). ( A ) The expression of the SRGAP1 gene before and after the GCs were transfected with the specific siRNA for 48 h was examined by qRT-PCR. The mRNA expression was normalized to that of the 18S rRNA gene; the values on the bar graphs represent the mean ± SEM of 10 hens (n = 10) from a representative experiment. ( B ) The expression levels of the SRGAP1 protein in the GCs with and without specific siRNA interference (RNAi) was detected by western blotting. β-actin was used as a loading control. ( C ) The immunoprecipitation (IP) with the PBD antibody was revealed by immunoblotting (IB) using monoclonal anti-CDC42 or anti-RAC1 antibodies (in the right-hand column). −, negative control; +, SLIT2 silencing group. All blots were cropped, and the gels were run under the same experimental conditions. ( D ) The expression levels of GTP-bound CDC42 and RAC1 under SLIT2 silencing were determined by western blotting. β-actin was used as a loading control. For each group, the different superscript above the bar indicates that the difference was significant (P

    Journal: Scientific Reports

    Article Title: Inhibitory effect of SLIT2 on granulosa cell proliferation mediated by the CDC42-PAKs-ERK1/2 MAPK pathway in the prehierarchical follicles of the chicken ovary

    doi: 10.1038/s41598-018-27601-z

    Figure Lengend Snippet: Effects of silencing SLIT2 on SRGAP1 expression and the GTPase activity of CDC42 and RAC1 in GCs. The granulosa cells were transfected with specific siRNAs targeting the SLIT2 gene, scrambled siRNA (negative control) or no siRNA (blank control). ( A ) The expression of the SRGAP1 gene before and after the GCs were transfected with the specific siRNA for 48 h was examined by qRT-PCR. The mRNA expression was normalized to that of the 18S rRNA gene; the values on the bar graphs represent the mean ± SEM of 10 hens (n = 10) from a representative experiment. ( B ) The expression levels of the SRGAP1 protein in the GCs with and without specific siRNA interference (RNAi) was detected by western blotting. β-actin was used as a loading control. ( C ) The immunoprecipitation (IP) with the PBD antibody was revealed by immunoblotting (IB) using monoclonal anti-CDC42 or anti-RAC1 antibodies (in the right-hand column). −, negative control; +, SLIT2 silencing group. All blots were cropped, and the gels were run under the same experimental conditions. ( D ) The expression levels of GTP-bound CDC42 and RAC1 under SLIT2 silencing were determined by western blotting. β-actin was used as a loading control. For each group, the different superscript above the bar indicates that the difference was significant (P

    Article Snippet: Conversely, the RNAi-mediated knockdown of SLIT2 resulted in significantly enhanced phosphorylation levels of the B-RAF, RAF1, MEK1/2 and ERK1/2 kinases in the Cs (P < 0.01, Fig. ).

    Techniques: Expressing, Activity Assay, Transfection, Negative Control, Quantitative RT-PCR, Western Blot, Immunoprecipitation

    Knockdown of Vti1a, syntaxin 6, and Vti1b by RNAi. HeLa cells were treated with a pool of siRNAs for Vti1a ( A–G ), syntaxin 6 ( A and H–M ), or Vti1b ( A and N–S ), or control siRNAs ( A and T–Y ), as described under “Experimental

    Journal: The Journal of Biological Chemistry

    Article Title: Differential Effects of Depletion of ARL1 and ARFRP1 on Membrane Trafficking between the trans-Golgi Network and Endosomes

    doi: 10.1074/jbc.M900847200

    Figure Lengend Snippet: Knockdown of Vti1a, syntaxin 6, and Vti1b by RNAi. HeLa cells were treated with a pool of siRNAs for Vti1a ( A–G ), syntaxin 6 ( A and H–M ), or Vti1b ( A and N–S ), or control siRNAs ( A and T–Y ), as described under “Experimental

    Article Snippet: More recently, RNAi-mediated knockdown of syntaxin 16 was shown to inhibit StxB transport ( , ).

    Techniques: