Article Title: The SUMO Conjugase Ubc9 Protects Dopaminergic Cells from Cytotoxicity and Enhances the Stability of α-Synuclein in Parkinson’s Disease Models
Figure Lengend Snippet: Ubc9 expression protects N27 cells from MPP+ or PFF-induced toxicity, enhancing cell viability and reducing cytotoxicity. A , MPP+ exposure reduces the number of viable cells in EGFP cells in a dose-dependent manner, whereas Ubc9 overexpression protects N27 cells from MPP+ toxicity in MTT assay. B , In LDH assay, Ubc9 overexpression reduces the toxic effect derived from MPP+ exposure. C , In MTT assay, Ubc9 knock-down by RNAi exacerbates the cell viability induced by MPP+ exposure, compared with NC1 random cocktail control. D , In LDH assay, Ubc9-RNAi significantly increases the cytotoxicity derived from MPP+ treatment. E , In MTT assay, PFF treatment reduces cell viability in EGFP cells compared with WGA/GluNAc-treated control, while Ubc9 overexpression ameliorates the toxic effect from PFF. F , In LDH assay, PFF-induced cytotoxicity was suppressed by Ubc9 overexpression. G , In MTT assay, Ubc9-RNAi further exacerbates the cell viability induced by PFF treatment, compared with NC1 random cocktail control with WGA/GluNAc. H , In LDH assay, Ubc9 knock-down substantially enhances the cytotoxicity derived from PFF treatment compared with NC1 control. All the treatments were exposed for 24 h, and each dot represents the number of experiments and each experiment was performed in triplicate. Statistical analysis was applied using two-way ANOVA, Tukey’s post hoc test. Scattered dot plots represent mean ± SEM; * p
Article Snippet: Cell lines with plasmids or RNAi treatment N27 rat dopaminergic parental cell line (SCC048, EMD Millipore) was used to generate enhanced green fluorescent protein (EGFP) or Ubc9-EGFP-overexpressing (Ubc9-OE) stable cell lines by transfection.
Techniques: Expressing, Over Expression, MTT Assay, Lactate Dehydrogenase Assay, Derivative Assay, Whole Genome Amplification