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  • 95
    Qiagen rneasy plant mini kit
    Profile of total RNA isolated by using five commercial kits. a Denaturing gel electrophoresis of total RNA isolated from peach. b Denaturing gel electrophoresis of total RNA from grapevine leaves. 50 mg of young peach and grapevine leaves (indicated as Y), and mature (M) grapevine leaves was used in RNA isolation with Spectrum™ Plant Total RNA kit (Sigma), <t>RNeasy</t> Plant mini kit (Qiagen), Plant/fungi total RNA kit (Norgen), AccuPrep viral RNA extraction kit (Bioneer) and <t>TRIzol</t> Reagent (Life Technologies). The total RNA yield (μg), A260/A280 and A260/A230 ratios averaged from two replicates are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows. c Capillary electrophoresis of total RNA with Agilent Bioanalyzer. One ml of each of the total RNA preparations isolated using these five systems was used for the analysis with an Agilent Bioanalyzer 2100 equipped with an RNA Nano chip
    Rneasy Plant Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 28897 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore rna extraction kits
    Profile of total RNA isolated by using five commercial kits. a Denaturing gel electrophoresis of total RNA isolated from peach. b Denaturing gel electrophoresis of total RNA from grapevine leaves. 50 mg of young peach and grapevine leaves (indicated as Y), and mature (M) grapevine leaves was used in RNA isolation with Spectrum™ Plant Total RNA kit (Sigma), <t>RNeasy</t> Plant mini kit (Qiagen), Plant/fungi total RNA kit (Norgen), AccuPrep viral RNA extraction kit (Bioneer) and <t>TRIzol</t> Reagent (Life Technologies). The total RNA yield (μg), A260/A280 and A260/A230 ratios averaged from two replicates are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows. c Capillary electrophoresis of total RNA with Agilent Bioanalyzer. One ml of each of the total RNA preparations isolated using these five systems was used for the analysis with an Agilent Bioanalyzer 2100 equipped with an RNA Nano chip
    Rna Extraction Kits, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Qiagen mini plant rna extraction kit
    Profile of total RNA isolated by using five commercial kits. a Denaturing gel electrophoresis of total RNA isolated from peach. b Denaturing gel electrophoresis of total RNA from grapevine leaves. 50 mg of young peach and grapevine leaves (indicated as Y), and mature (M) grapevine leaves was used in RNA isolation with Spectrum™ Plant Total RNA kit (Sigma), <t>RNeasy</t> Plant mini kit (Qiagen), Plant/fungi total RNA kit (Norgen), AccuPrep viral RNA extraction kit (Bioneer) and <t>TRIzol</t> Reagent (Life Technologies). The total RNA yield (μg), A260/A280 and A260/A230 ratios averaged from two replicates are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows. c Capillary electrophoresis of total RNA with Agilent Bioanalyzer. One ml of each of the total RNA preparations isolated using these five systems was used for the analysis with an Agilent Bioanalyzer 2100 equipped with an RNA Nano chip
    Mini Plant Rna Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Qiagen rneasy mini kit
    Profile of total RNA isolated by using five commercial kits. a Denaturing gel electrophoresis of total RNA isolated from peach. b Denaturing gel electrophoresis of total RNA from grapevine leaves. 50 mg of young peach and grapevine leaves (indicated as Y), and mature (M) grapevine leaves was used in RNA isolation with Spectrum™ Plant Total RNA kit (Sigma), <t>RNeasy</t> Plant mini kit (Qiagen), Plant/fungi total RNA kit (Norgen), AccuPrep viral RNA extraction kit (Bioneer) and <t>TRIzol</t> Reagent (Life Technologies). The total RNA yield (μg), A260/A280 and A260/A230 ratios averaged from two replicates are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows. c Capillary electrophoresis of total RNA with Agilent Bioanalyzer. One ml of each of the total RNA preparations isolated using these five systems was used for the analysis with an Agilent Bioanalyzer 2100 equipped with an RNA Nano chip
    Rneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 266299 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy mini kit/product/Qiagen
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    95
    Qiagen dneasy plant mini kit
    Profile of total RNA isolated by using five commercial kits. a Denaturing gel electrophoresis of total RNA isolated from peach. b Denaturing gel electrophoresis of total RNA from grapevine leaves. 50 mg of young peach and grapevine leaves (indicated as Y), and mature (M) grapevine leaves was used in RNA isolation with Spectrum™ Plant Total RNA kit (Sigma), <t>RNeasy</t> Plant mini kit (Qiagen), Plant/fungi total RNA kit (Norgen), AccuPrep viral RNA extraction kit (Bioneer) and <t>TRIzol</t> Reagent (Life Technologies). The total RNA yield (μg), A260/A280 and A260/A230 ratios averaged from two replicates are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows. c Capillary electrophoresis of total RNA with Agilent Bioanalyzer. One ml of each of the total RNA preparations isolated using these five systems was used for the analysis with an Agilent Bioanalyzer 2100 equipped with an RNA Nano chip
    Dneasy Plant Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 14603 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Qiagen rnaeasy plant lipid tissue mini kit
    Profile of total RNA isolated by using five commercial kits. a Denaturing gel electrophoresis of total RNA isolated from peach. b Denaturing gel electrophoresis of total RNA from grapevine leaves. 50 mg of young peach and grapevine leaves (indicated as Y), and mature (M) grapevine leaves was used in RNA isolation with Spectrum™ Plant Total RNA kit (Sigma), <t>RNeasy</t> Plant mini kit (Qiagen), Plant/fungi total RNA kit (Norgen), AccuPrep viral RNA extraction kit (Bioneer) and <t>TRIzol</t> Reagent (Life Technologies). The total RNA yield (μg), A260/A280 and A260/A230 ratios averaged from two replicates are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows. c Capillary electrophoresis of total RNA with Agilent Bioanalyzer. One ml of each of the total RNA preparations isolated using these five systems was used for the analysis with an Agilent Bioanalyzer 2100 equipped with an RNA Nano chip
    Rnaeasy Plant Lipid Tissue Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher rneasy plant mini kit
    Profile of total RNA isolated by using five commercial kits. a Denaturing gel electrophoresis of total RNA isolated from peach. b Denaturing gel electrophoresis of total RNA from grapevine leaves. 50 mg of young peach and grapevine leaves (indicated as Y), and mature (M) grapevine leaves was used in RNA isolation with Spectrum™ Plant Total RNA kit (Sigma), <t>RNeasy</t> Plant mini kit (Qiagen), Plant/fungi total RNA kit (Norgen), AccuPrep viral RNA extraction kit (Bioneer) and <t>TRIzol</t> Reagent (Life Technologies). The total RNA yield (μg), A260/A280 and A260/A230 ratios averaged from two replicates are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows. c Capillary electrophoresis of total RNA with Agilent Bioanalyzer. One ml of each of the total RNA preparations isolated using these five systems was used for the analysis with an Agilent Bioanalyzer 2100 equipped with an RNA Nano chip
    Rneasy Plant Mini Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Qiagen mirneasy mini kit
    Profile of total RNA isolated by using five commercial kits. a Denaturing gel electrophoresis of total RNA isolated from peach. b Denaturing gel electrophoresis of total RNA from grapevine leaves. 50 mg of young peach and grapevine leaves (indicated as Y), and mature (M) grapevine leaves was used in RNA isolation with Spectrum™ Plant Total RNA kit (Sigma), <t>RNeasy</t> Plant mini kit (Qiagen), Plant/fungi total RNA kit (Norgen), AccuPrep viral RNA extraction kit (Bioneer) and <t>TRIzol</t> Reagent (Life Technologies). The total RNA yield (μg), A260/A280 and A260/A230 ratios averaged from two replicates are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows. c Capillary electrophoresis of total RNA with Agilent Bioanalyzer. One ml of each of the total RNA preparations isolated using these five systems was used for the analysis with an Agilent Bioanalyzer 2100 equipped with an RNA Nano chip
    Mirneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 25921 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirneasy mini kit/product/Qiagen
    Average 95 stars, based on 25921 article reviews
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    rpe  (Qiagen)
    95
    Qiagen rpe
    Development of RNA extraction protocol . A : RNA extracted from Arabidopsis leaves. Lane 1, RNA extraction using Logemann et al.'s protocol [ 4 ]. Lanes 2 and 3, RNA obtained by transfer through a plasmid DNA extraction column (QIAprep Spin Miniprep Kit). Lanes 4 and 5, RNA obtained from transfer through an RNA collection column (RNeasy). EtOH, 96% ethanol; M, DNA 1-kb ladder. B : RNA yield following extraction in 8 M guanidine hydrochloride buffer and 0.5 volume of 96% EtOH and transfer through a plasmid DNA extraction column (QIAprep Spin Miniprep Kit), followed by 2-3 washes in: (1) <t>RW1</t> × 2 and <t>RPE</t> (RNeasy Plant Mini Kit), (2) 3 M Na-acetate and 70-75% EtOH; (3) two washes with 96% EtOH; (4) 3 M Na-acetate and PB (QIAprep); (5) two washes with PB. RNA yield was measured spectrophotometrically.
    Rpe, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Qiagen allprep dna rna mini kit
    Development of RNA extraction protocol . A : RNA extracted from Arabidopsis leaves. Lane 1, RNA extraction using Logemann et al.'s protocol [ 4 ]. Lanes 2 and 3, RNA obtained by transfer through a plasmid DNA extraction column (QIAprep Spin Miniprep Kit). Lanes 4 and 5, RNA obtained from transfer through an RNA collection column (RNeasy). EtOH, 96% ethanol; M, DNA 1-kb ladder. B : RNA yield following extraction in 8 M guanidine hydrochloride buffer and 0.5 volume of 96% EtOH and transfer through a plasmid DNA extraction column (QIAprep Spin Miniprep Kit), followed by 2-3 washes in: (1) <t>RW1</t> × 2 and <t>RPE</t> (RNeasy Plant Mini Kit), (2) 3 M Na-acetate and 70-75% EtOH; (3) two washes with 96% EtOH; (4) 3 M Na-acetate and PB (QIAprep); (5) two washes with PB. RNA yield was measured spectrophotometrically.
    Allprep Dna Rna Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 5605 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Qiagen rnase free dnase set
    Development of RNA extraction protocol . A : RNA extracted from Arabidopsis leaves. Lane 1, RNA extraction using Logemann et al.'s protocol [ 4 ]. Lanes 2 and 3, RNA obtained by transfer through a plasmid DNA extraction column (QIAprep Spin Miniprep Kit). Lanes 4 and 5, RNA obtained from transfer through an RNA collection column (RNeasy). EtOH, 96% ethanol; M, DNA 1-kb ladder. B : RNA yield following extraction in 8 M guanidine hydrochloride buffer and 0.5 volume of 96% EtOH and transfer through a plasmid DNA extraction column (QIAprep Spin Miniprep Kit), followed by 2-3 washes in: (1) <t>RW1</t> × 2 and <t>RPE</t> (RNeasy Plant Mini Kit), (2) 3 M Na-acetate and 70-75% EtOH; (3) two washes with 96% EtOH; (4) 3 M Na-acetate and PB (QIAprep); (5) two washes with PB. RNA yield was measured spectrophotometrically.
    Rnase Free Dnase Set, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 18433 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Qiagen one step rt pcr kit
    Development of RNA extraction protocol . A : RNA extracted from Arabidopsis leaves. Lane 1, RNA extraction using Logemann et al.'s protocol [ 4 ]. Lanes 2 and 3, RNA obtained by transfer through a plasmid DNA extraction column (QIAprep Spin Miniprep Kit). Lanes 4 and 5, RNA obtained from transfer through an RNA collection column (RNeasy). EtOH, 96% ethanol; M, DNA 1-kb ladder. B : RNA yield following extraction in 8 M guanidine hydrochloride buffer and 0.5 volume of 96% EtOH and transfer through a plasmid DNA extraction column (QIAprep Spin Miniprep Kit), followed by 2-3 washes in: (1) <t>RW1</t> × 2 and <t>RPE</t> (RNeasy Plant Mini Kit), (2) 3 M Na-acetate and 70-75% EtOH; (3) two washes with 96% EtOH; (4) 3 M Na-acetate and PB (QIAprep); (5) two washes with PB. RNA yield was measured spectrophotometrically.
    One Step Rt Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 9238 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Profile of total RNA isolated by using five commercial kits. a Denaturing gel electrophoresis of total RNA isolated from peach. b Denaturing gel electrophoresis of total RNA from grapevine leaves. 50 mg of young peach and grapevine leaves (indicated as Y), and mature (M) grapevine leaves was used in RNA isolation with Spectrum™ Plant Total RNA kit (Sigma), RNeasy Plant mini kit (Qiagen), Plant/fungi total RNA kit (Norgen), AccuPrep viral RNA extraction kit (Bioneer) and TRIzol Reagent (Life Technologies). The total RNA yield (μg), A260/A280 and A260/A230 ratios averaged from two replicates are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows. c Capillary electrophoresis of total RNA with Agilent Bioanalyzer. One ml of each of the total RNA preparations isolated using these five systems was used for the analysis with an Agilent Bioanalyzer 2100 equipped with an RNA Nano chip

    Journal: Virology Journal

    Article Title: A highly effective and versatile technology for the isolation of RNAs from grapevines and other woody perennials for use in virus diagnostics

    doi: 10.1186/s12985-015-0376-3

    Figure Lengend Snippet: Profile of total RNA isolated by using five commercial kits. a Denaturing gel electrophoresis of total RNA isolated from peach. b Denaturing gel electrophoresis of total RNA from grapevine leaves. 50 mg of young peach and grapevine leaves (indicated as Y), and mature (M) grapevine leaves was used in RNA isolation with Spectrum™ Plant Total RNA kit (Sigma), RNeasy Plant mini kit (Qiagen), Plant/fungi total RNA kit (Norgen), AccuPrep viral RNA extraction kit (Bioneer) and TRIzol Reagent (Life Technologies). The total RNA yield (μg), A260/A280 and A260/A230 ratios averaged from two replicates are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows. c Capillary electrophoresis of total RNA with Agilent Bioanalyzer. One ml of each of the total RNA preparations isolated using these five systems was used for the analysis with an Agilent Bioanalyzer 2100 equipped with an RNA Nano chip

    Article Snippet: These five kits are TRIzol Reagent (Life Technologies), RNeasy Plant mini kit (Qiagen), Spectrum™ Plant Total RNA kit (Sigma), AccuPrep viral RNA extraction kit (Bioneer) and Plant/fungi total RNA kit (Norgen BioTek).

    Techniques: Isolation, Nucleic Acid Electrophoresis, RNA Extraction, Electrophoresis, Chromatin Immunoprecipitation

    Development of RNA extraction protocol . A : RNA extracted from Arabidopsis leaves. Lane 1, RNA extraction using Logemann et al.'s protocol [ 4 ]. Lanes 2 and 3, RNA obtained by transfer through a plasmid DNA extraction column (QIAprep Spin Miniprep Kit). Lanes 4 and 5, RNA obtained from transfer through an RNA collection column (RNeasy). EtOH, 96% ethanol; M, DNA 1-kb ladder. B : RNA yield following extraction in 8 M guanidine hydrochloride buffer and 0.5 volume of 96% EtOH and transfer through a plasmid DNA extraction column (QIAprep Spin Miniprep Kit), followed by 2-3 washes in: (1) RW1 × 2 and RPE (RNeasy Plant Mini Kit), (2) 3 M Na-acetate and 70-75% EtOH; (3) two washes with 96% EtOH; (4) 3 M Na-acetate and PB (QIAprep); (5) two washes with PB. RNA yield was measured spectrophotometrically.

    Journal: BMC Research Notes

    Article Title: LogSpin: a simple, economical and fast method for RNA isolation from infected or healthy plants and other eukaryotic tissues

    doi: 10.1186/1756-0500-5-45

    Figure Lengend Snippet: Development of RNA extraction protocol . A : RNA extracted from Arabidopsis leaves. Lane 1, RNA extraction using Logemann et al.'s protocol [ 4 ]. Lanes 2 and 3, RNA obtained by transfer through a plasmid DNA extraction column (QIAprep Spin Miniprep Kit). Lanes 4 and 5, RNA obtained from transfer through an RNA collection column (RNeasy). EtOH, 96% ethanol; M, DNA 1-kb ladder. B : RNA yield following extraction in 8 M guanidine hydrochloride buffer and 0.5 volume of 96% EtOH and transfer through a plasmid DNA extraction column (QIAprep Spin Miniprep Kit), followed by 2-3 washes in: (1) RW1 × 2 and RPE (RNeasy Plant Mini Kit), (2) 3 M Na-acetate and 70-75% EtOH; (3) two washes with 96% EtOH; (4) 3 M Na-acetate and PB (QIAprep); (5) two washes with PB. RNA yield was measured spectrophotometrically.

    Article Snippet: Once we established that plasmid DNA spin columns can bind RNA with the use of commercial solutions (RNeasy Plant Mini Kit), we compared the efficiencies of five solution combinations for the washing step: (1) RW1 and RPE (RNeasy Plant Mini Kit buffers, Qiagen), (2) 3 M Na-acetate and 75% EtOH, (3) 96% EtOH, (4) 3 M Na-acetate and PB buffer (QIAprep binding buffer, Qiagen), and (5) PB only (Figure ).

    Techniques: RNA Extraction, Plasmid Preparation, DNA Extraction