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  • 97
    Thermo Fisher rna levels
    5′ RACE analyses of transcription start sites 3′ to HSI in the hGH BAC and derivative transgenes reveals consistent spacing between the HSI core determinants and the TSS cluster. <t>RNA</t> was isolated from the pituitaries of mice carrying each of the 5 indicated transgenes (Figure 1 ). In each case, <t>cDNA</t> synthesis was primed at the site labeled 1 (left facing arrow), poly G tails were added to the 3′ end of the cDNA, and the product was then amplified between an adapter-polyC 17 primer and a nested primer (arrow 2). The amplified PCR products were cloned and individually sequenced. Each triangle indicates the 5′ terminus of an individual clone and the results were grouped within 50 bp windows. The total numbers of cDNAs containing an additional non-templated terminal G (corresponding to the 5′-capped structure; filled triangles) are shown, and the total numbers of cDNAs with and without the nontemplated G are included in parentheses. The arrows indicate the distance between the HSI core and the center of the most proximal TSS cluster. hGH BAC , 123 kb unmodified human transgenic mouse line (two copies) ( 26 ); CDΔ0.7/hGH BAC , 0.7 kb deletion of 0.5 kb promoter region and exon 1 from hGH BAC (14 copies) ( 25 ); CDΔ1.6/hGH BAC , 1.6 kb deletion of 0.5 kb promoter through exon 2 from the hGH BAC (three copies) ( 23 ); −8.0CD79bΔ1.6 , 1.6 kb deletion of hCD79b promoter through exon 2 from the −8.0CD79b (6 copies); λΔCD/hGH BAC , 3.8 kb λ gene segment replacing hCD79b from hGH BAC (five copies) ( 23 ).
    Rna Levels, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher micrornas
    5′ RACE analyses of transcription start sites 3′ to HSI in the hGH BAC and derivative transgenes reveals consistent spacing between the HSI core determinants and the TSS cluster. <t>RNA</t> was isolated from the pituitaries of mice carrying each of the 5 indicated transgenes (Figure 1 ). In each case, <t>cDNA</t> synthesis was primed at the site labeled 1 (left facing arrow), poly G tails were added to the 3′ end of the cDNA, and the product was then amplified between an adapter-polyC 17 primer and a nested primer (arrow 2). The amplified PCR products were cloned and individually sequenced. Each triangle indicates the 5′ terminus of an individual clone and the results were grouped within 50 bp windows. The total numbers of cDNAs containing an additional non-templated terminal G (corresponding to the 5′-capped structure; filled triangles) are shown, and the total numbers of cDNAs with and without the nontemplated G are included in parentheses. The arrows indicate the distance between the HSI core and the center of the most proximal TSS cluster. hGH BAC , 123 kb unmodified human transgenic mouse line (two copies) ( 26 ); CDΔ0.7/hGH BAC , 0.7 kb deletion of 0.5 kb promoter region and exon 1 from hGH BAC (14 copies) ( 25 ); CDΔ1.6/hGH BAC , 1.6 kb deletion of 0.5 kb promoter through exon 2 from the hGH BAC (three copies) ( 23 ); −8.0CD79bΔ1.6 , 1.6 kb deletion of hCD79b promoter through exon 2 from the −8.0CD79b (6 copies); λΔCD/hGH BAC , 3.8 kb λ gene segment replacing hCD79b from hGH BAC (five copies) ( 23 ).
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    99
    Thermo Fisher rna
    5′ RACE analyses of transcription start sites 3′ to HSI in the hGH BAC and derivative transgenes reveals consistent spacing between the HSI core determinants and the TSS cluster. <t>RNA</t> was isolated from the pituitaries of mice carrying each of the 5 indicated transgenes (Figure 1 ). In each case, <t>cDNA</t> synthesis was primed at the site labeled 1 (left facing arrow), poly G tails were added to the 3′ end of the cDNA, and the product was then amplified between an adapter-polyC 17 primer and a nested primer (arrow 2). The amplified PCR products were cloned and individually sequenced. Each triangle indicates the 5′ terminus of an individual clone and the results were grouped within 50 bp windows. The total numbers of cDNAs containing an additional non-templated terminal G (corresponding to the 5′-capped structure; filled triangles) are shown, and the total numbers of cDNAs with and without the nontemplated G are included in parentheses. The arrows indicate the distance between the HSI core and the center of the most proximal TSS cluster. hGH BAC , 123 kb unmodified human transgenic mouse line (two copies) ( 26 ); CDΔ0.7/hGH BAC , 0.7 kb deletion of 0.5 kb promoter region and exon 1 from hGH BAC (14 copies) ( 25 ); CDΔ1.6/hGH BAC , 1.6 kb deletion of 0.5 kb promoter through exon 2 from the hGH BAC (three copies) ( 23 ); −8.0CD79bΔ1.6 , 1.6 kb deletion of hCD79b promoter through exon 2 from the −8.0CD79b (6 copies); λΔCD/hGH BAC , 3.8 kb λ gene segment replacing hCD79b from hGH BAC (five copies) ( 23 ).
    Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher mrna levels
    <t>Galectin-8</t> Is Expressed in Lymphoid Tissues (A) qRT-PCR analysis of Galectin-8 ( Lgals8 ) <t>mRNA</t> levels in kidney, bone marrow (BM), spleen, lymph nodes (LNs), and liver of C56BL/6 WT mice. Values were normalized with respect to the BM condition for each mouse. n = 5 mice pooled from N = 3 independent experiments. Bar graphs indicate mean ± SEM. (B) Representative images of H E staining (left) and β-galactosidase staining (right) of LN cryosection from heterozygous mouse bearing a LacZ expression cassette on one allele of the Lgals8 locus. Arrowheads on the inset highlight β-galactosidase staining within the SCS area. Scale bar, 150 μm. (C) Representative images of serial lymph node cryosections stained for β-galactosidase (Galectin-8) and macrophages (Mac1) or B cells (B220). Scale bar, 200 μm. Zooms highlight the spatial localization of Galectin-8 together with macrophages and B cells at the SCS. Scale bar, 30 μm. .
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    Image Search Results


    5′ RACE analyses of transcription start sites 3′ to HSI in the hGH BAC and derivative transgenes reveals consistent spacing between the HSI core determinants and the TSS cluster. RNA was isolated from the pituitaries of mice carrying each of the 5 indicated transgenes (Figure 1 ). In each case, cDNA synthesis was primed at the site labeled 1 (left facing arrow), poly G tails were added to the 3′ end of the cDNA, and the product was then amplified between an adapter-polyC 17 primer and a nested primer (arrow 2). The amplified PCR products were cloned and individually sequenced. Each triangle indicates the 5′ terminus of an individual clone and the results were grouped within 50 bp windows. The total numbers of cDNAs containing an additional non-templated terminal G (corresponding to the 5′-capped structure; filled triangles) are shown, and the total numbers of cDNAs with and without the nontemplated G are included in parentheses. The arrows indicate the distance between the HSI core and the center of the most proximal TSS cluster. hGH BAC , 123 kb unmodified human transgenic mouse line (two copies) ( 26 ); CDΔ0.7/hGH BAC , 0.7 kb deletion of 0.5 kb promoter region and exon 1 from hGH BAC (14 copies) ( 25 ); CDΔ1.6/hGH BAC , 1.6 kb deletion of 0.5 kb promoter through exon 2 from the hGH BAC (three copies) ( 23 ); −8.0CD79bΔ1.6 , 1.6 kb deletion of hCD79b promoter through exon 2 from the −8.0CD79b (6 copies); λΔCD/hGH BAC , 3.8 kb λ gene segment replacing hCD79b from hGH BAC (five copies) ( 23 ).

    Journal: Nucleic Acids Research

    Article Title: Autonomous actions of the human growth hormone long-range enhancer

    doi: 10.1093/nar/gkv093

    Figure Lengend Snippet: 5′ RACE analyses of transcription start sites 3′ to HSI in the hGH BAC and derivative transgenes reveals consistent spacing between the HSI core determinants and the TSS cluster. RNA was isolated from the pituitaries of mice carrying each of the 5 indicated transgenes (Figure 1 ). In each case, cDNA synthesis was primed at the site labeled 1 (left facing arrow), poly G tails were added to the 3′ end of the cDNA, and the product was then amplified between an adapter-polyC 17 primer and a nested primer (arrow 2). The amplified PCR products were cloned and individually sequenced. Each triangle indicates the 5′ terminus of an individual clone and the results were grouped within 50 bp windows. The total numbers of cDNAs containing an additional non-templated terminal G (corresponding to the 5′-capped structure; filled triangles) are shown, and the total numbers of cDNAs with and without the nontemplated G are included in parentheses. The arrows indicate the distance between the HSI core and the center of the most proximal TSS cluster. hGH BAC , 123 kb unmodified human transgenic mouse line (two copies) ( 26 ); CDΔ0.7/hGH BAC , 0.7 kb deletion of 0.5 kb promoter region and exon 1 from hGH BAC (14 copies) ( 25 ); CDΔ1.6/hGH BAC , 1.6 kb deletion of 0.5 kb promoter through exon 2 from the hGH BAC (three copies) ( 23 ); −8.0CD79bΔ1.6 , 1.6 kb deletion of hCD79b promoter through exon 2 from the −8.0CD79b (6 copies); λΔCD/hGH BAC , 3.8 kb λ gene segment replacing hCD79b from hGH BAC (five copies) ( 23 ).

    Article Snippet: In all cases, 1 μg of purified RNA was reverse-transcribed using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) and RNA levels were assessed by real-time PCR using TaqMan Universal PCR Master mix (Applied Biosystems) and a 7900HT platform with corresponding probes (hCD79b , Hs00236881_m1; λ; mGAPDH , Mm99999915_g1).

    Techniques: BAC Assay, Isolation, Mouse Assay, Labeling, Amplification, Polymerase Chain Reaction, Clone Assay, Transgenic Assay

    Galectin-8 Is Expressed in Lymphoid Tissues (A) qRT-PCR analysis of Galectin-8 ( Lgals8 ) mRNA levels in kidney, bone marrow (BM), spleen, lymph nodes (LNs), and liver of C56BL/6 WT mice. Values were normalized with respect to the BM condition for each mouse. n = 5 mice pooled from N = 3 independent experiments. Bar graphs indicate mean ± SEM. (B) Representative images of H E staining (left) and β-galactosidase staining (right) of LN cryosection from heterozygous mouse bearing a LacZ expression cassette on one allele of the Lgals8 locus. Arrowheads on the inset highlight β-galactosidase staining within the SCS area. Scale bar, 150 μm. (C) Representative images of serial lymph node cryosections stained for β-galactosidase (Galectin-8) and macrophages (Mac1) or B cells (B220). Scale bar, 200 μm. Zooms highlight the spatial localization of Galectin-8 together with macrophages and B cells at the SCS. Scale bar, 30 μm. .

    Journal: Cell Reports

    Article Title: Galectin-8 Favors the Presentation of Surface-Tethered Antigens by Stabilizing the B Cell Immune Synapse

    doi: 10.1016/j.celrep.2018.11.052

    Figure Lengend Snippet: Galectin-8 Is Expressed in Lymphoid Tissues (A) qRT-PCR analysis of Galectin-8 ( Lgals8 ) mRNA levels in kidney, bone marrow (BM), spleen, lymph nodes (LNs), and liver of C56BL/6 WT mice. Values were normalized with respect to the BM condition for each mouse. n = 5 mice pooled from N = 3 independent experiments. Bar graphs indicate mean ± SEM. (B) Representative images of H E staining (left) and β-galactosidase staining (right) of LN cryosection from heterozygous mouse bearing a LacZ expression cassette on one allele of the Lgals8 locus. Arrowheads on the inset highlight β-galactosidase staining within the SCS area. Scale bar, 150 μm. (C) Representative images of serial lymph node cryosections stained for β-galactosidase (Galectin-8) and macrophages (Mac1) or B cells (B220). Scale bar, 200 μm. Zooms highlight the spatial localization of Galectin-8 together with macrophages and B cells at the SCS. Scale bar, 30 μm. .

    Article Snippet: Galectin-8 (primer: Mm01332239-m1 Lgals 8, Life Technologies) mRNA levels were assessed using TaqMan Gene Expression Master Mix (Applied Biosystems) according to manufacturer’s recommendations.

    Techniques: Quantitative RT-PCR, Mouse Assay, Staining, Expressing