rna isolation columns Search Results


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  • 94
    Thermo Fisher purelink rna isolation columns
    Purelink Rna Isolation Columns, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore rna
    Analysis of <t>RNA</t> association with the Era protein of S. pneumoniae. E. coli rRNAs were isolated as described in Materials and Methods. All samples were electrophoresed on a 1.5% agarose gel containing ethidium bromide. Lane 1, DNA standards (100-bp increments from bottom to top; Gibco BRL); lanes 2 and 3, E. coli rRNA untreated and treated with <t>RNase</t> A, respectively; lanes 4 and 5, phenol-chloroform-extracted material from a purified GST-Era protein preparation untreated and treated with RNase A, respectively; lanes 6 and 7, a purified GST-Era protein preparation untreated and treated with RNase A, respectively; lane 8, phenol-chloroform-extracted material from a purified GST-Era protein preparation treated with DNase.
    Rna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 6042 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore e coli transfer rna
    Quantitative RT-PCR for total and pull-down <t>RNA</t> fraction. Total cellular RNA was isolated from Neuro-2a cells treated with 0.1mM or 1mM H 2 O 2 for <t>30min.</t> ARP-RNA (1μg of total ARP-derivatized RNA) was reverse transcribed with random hexamers (left
    E Coli Transfer Rna, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore yeast trna
    Quantitative RT-PCR for total and pull-down <t>RNA</t> fraction. Total cellular RNA was isolated from Neuro-2a cells treated with 0.1mM or 1mM H 2 O 2 for <t>30min.</t> ARP-RNA (1μg of total ARP-derivatized RNA) was reverse transcribed with random hexamers (left
    Yeast Trna, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 3414 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore column fractions rna content
    Quantitative RT-PCR for total and pull-down <t>RNA</t> fraction. Total cellular RNA was isolated from Neuro-2a cells treated with 0.1mM or 1mM H 2 O 2 for <t>30min.</t> ARP-RNA (1μg of total ARP-derivatized RNA) was reverse transcribed with random hexamers (left
    Column Fractions Rna Content, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore trna
    Quantitative RT-PCR for total and pull-down <t>RNA</t> fraction. Total cellular RNA was isolated from Neuro-2a cells treated with 0.1mM or 1mM H 2 O 2 for <t>30min.</t> ARP-RNA (1μg of total ARP-derivatized RNA) was reverse transcribed with random hexamers (left
    Trna, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 2442 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Omega Bio-tek rna isolation columns
    Quantitative RT-PCR for total and pull-down <t>RNA</t> fraction. Total cellular RNA was isolated from Neuro-2a cells treated with 0.1mM or 1mM H 2 O 2 for <t>30min.</t> ARP-RNA (1μg of total ARP-derivatized RNA) was reverse transcribed with random hexamers (left
    Rna Isolation Columns, supplied by Omega Bio-tek, used in various techniques. Bioz Stars score: 88/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MACHEREY NAGEL rna isolation columns
    Quantitative RT-PCR for total and pull-down <t>RNA</t> fraction. Total cellular RNA was isolated from Neuro-2a cells treated with 0.1mM or 1mM H 2 O 2 for <t>30min.</t> ARP-RNA (1μg of total ARP-derivatized RNA) was reverse transcribed with random hexamers (left
    Rna Isolation Columns, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 88/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega rna isolation columns
    Quantitative RT-PCR for total and pull-down <t>RNA</t> fraction. Total cellular RNA was isolated from Neuro-2a cells treated with 0.1mM or 1mM H 2 O 2 for <t>30min.</t> ARP-RNA (1μg of total ARP-derivatized RNA) was reverse transcribed with random hexamers (left
    Rna Isolation Columns, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen rna isolation columns
    Quantitative RT-PCR for total and pull-down <t>RNA</t> fraction. Total cellular RNA was isolated from Neuro-2a cells treated with 0.1mM or 1mM H 2 O 2 for <t>30min.</t> ARP-RNA (1μg of total ARP-derivatized RNA) was reverse transcribed with random hexamers (left
    Rna Isolation Columns, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MACHEREY NAGEL nucleospin rna isolation columns
    Quantitative RT-PCR for total and pull-down <t>RNA</t> fraction. Total cellular RNA was isolated from Neuro-2a cells treated with 0.1mM or 1mM H 2 O 2 for <t>30min.</t> ARP-RNA (1μg of total ARP-derivatized RNA) was reverse transcribed with random hexamers (left
    Nucleospin Rna Isolation Columns, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher picopure rna isolation columns
    Quantitative RT-PCR for total and pull-down <t>RNA</t> fraction. Total cellular RNA was isolated from Neuro-2a cells treated with 0.1mM or 1mM H 2 O 2 for <t>30min.</t> ARP-RNA (1μg of total ARP-derivatized RNA) was reverse transcribed with random hexamers (left
    Picopure Rna Isolation Columns, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen rneasy rna isolation column
    Quantitative RT-PCR for total and pull-down <t>RNA</t> fraction. Total cellular RNA was isolated from Neuro-2a cells treated with 0.1mM or 1mM H 2 O 2 for <t>30min.</t> ARP-RNA (1μg of total ARP-derivatized RNA) was reverse transcribed with random hexamers (left
    Rneasy Rna Isolation Column, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen rneasy rna isolation columns
    Quantitative RT-PCR for total and pull-down <t>RNA</t> fraction. Total cellular RNA was isolated from Neuro-2a cells treated with 0.1mM or 1mM H 2 O 2 for <t>30min.</t> ARP-RNA (1μg of total ARP-derivatized RNA) was reverse transcribed with random hexamers (left
    Rneasy Rna Isolation Columns, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene column based rna isolation kit
    Quantitative RT-PCR for total and pull-down <t>RNA</t> fraction. Total cellular RNA was isolated from Neuro-2a cells treated with 0.1mM or 1mM H 2 O 2 for <t>30min.</t> ARP-RNA (1μg of total ARP-derivatized RNA) was reverse transcribed with random hexamers (left
    Column Based Rna Isolation Kit, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Omega Bio-tek ezna rna isolation kit columns
    Quantitative RT-PCR for total and pull-down <t>RNA</t> fraction. Total cellular RNA was isolated from Neuro-2a cells treated with 0.1mM or 1mM H 2 O 2 for <t>30min.</t> ARP-RNA (1μg of total ARP-derivatized RNA) was reverse transcribed with random hexamers (left
    Ezna Rna Isolation Kit Columns, supplied by Omega Bio-tek, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche high pure rna isolation kit columns
    Quantitative RT-PCR for total and pull-down <t>RNA</t> fraction. Total cellular RNA was isolated from Neuro-2a cells treated with 0.1mM or 1mM H 2 O 2 for <t>30min.</t> ARP-RNA (1μg of total ARP-derivatized RNA) was reverse transcribed with random hexamers (left
    High Pure Rna Isolation Kit Columns, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher column based rna isolation kit
    Comparison of different storage temperature and trehalose for FTA Elute card stored for 2 days. ( A ) Untreated or 50 mg/ml trehalose-treated 6 mm FTA Elute card disc punch-out spotted with 20 µl serum were dried and stored at RT, 4 °C or −20 °C for 2 days before <t>RNA</t> was extracted using <t>QIAzol</t> lysis reagent. 10 miRNAs were quantified by RT-qPCR and their copy numbers were presented. Neat refers to copy number of each individual miRNA obtained from 20 µl serum extracted directly using QIAzol lysis reagent. The individual % miRNA recovery was compared between untreated and trehalose-treated FTA Elute card disc punch-out. ( B ) Average % miRNA recovery of all 10 miRNAs was calculated and plotted to compare the effect of pre-treatment of FTA Elute card disc punch-out with 50mg/ml trehalose. Statistical analyses were performed with one-way ANOVA, followed by Bonferroni’s pairwise comparisons test data between selected pairs. Each experimental condition was carried out thrice and data were presented as mean ± SEM (*** P ≤ 0.001; **P ≤ 0.01; * P ≤ 0.05, n = 3).
    Column Based Rna Isolation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Analysis of RNA association with the Era protein of S. pneumoniae. E. coli rRNAs were isolated as described in Materials and Methods. All samples were electrophoresed on a 1.5% agarose gel containing ethidium bromide. Lane 1, DNA standards (100-bp increments from bottom to top; Gibco BRL); lanes 2 and 3, E. coli rRNA untreated and treated with RNase A, respectively; lanes 4 and 5, phenol-chloroform-extracted material from a purified GST-Era protein preparation untreated and treated with RNase A, respectively; lanes 6 and 7, a purified GST-Era protein preparation untreated and treated with RNase A, respectively; lane 8, phenol-chloroform-extracted material from a purified GST-Era protein preparation treated with DNase.

    Journal: Journal of Bacteriology

    Article Title: 16S rRNA Is Bound to Era of Streptococcus pneumoniae

    doi:

    Figure Lengend Snippet: Analysis of RNA association with the Era protein of S. pneumoniae. E. coli rRNAs were isolated as described in Materials and Methods. All samples were electrophoresed on a 1.5% agarose gel containing ethidium bromide. Lane 1, DNA standards (100-bp increments from bottom to top; Gibco BRL); lanes 2 and 3, E. coli rRNA untreated and treated with RNase A, respectively; lanes 4 and 5, phenol-chloroform-extracted material from a purified GST-Era protein preparation untreated and treated with RNase A, respectively; lanes 6 and 7, a purified GST-Era protein preparation untreated and treated with RNase A, respectively; lane 8, phenol-chloroform-extracted material from a purified GST-Era protein preparation treated with DNase.

    Article Snippet: The peak fractions containing Era or RNA were treated with RNase A (Sigma) or DNase I (Gibco BRL) as indicated in the figure legends.

    Techniques: Isolation, Agarose Gel Electrophoresis, Purification

    Analysis of Era-RNA complex formation in a crude extract of S. pneumoniae by gel filtration column chromatography. A crude extract of S. pneumoniae was prepared, untreated (A) or treated with RNase A (1 mg/ml) (B), and subjected to chromatography on a gel filtration column as described in Materials and Methods. The presence of Era in the fractions collected was detected by Western blotting analysis with polyclonal antibodies prepared against the native Era protein of S. pneumoniae ). The intensity of each band was quantified by scanning as described in Materials and Methods. Lanes 1 and 11, molecular mass markers and purified Era of S. pneumoniae (30 ng), respectively; lanes 2 to 10, fractions 23 to 31, respectively; lanes 12 to 19, fractions 39 to 46, respectively.

    Journal: Journal of Bacteriology

    Article Title: 16S rRNA Is Bound to Era of Streptococcus pneumoniae

    doi:

    Figure Lengend Snippet: Analysis of Era-RNA complex formation in a crude extract of S. pneumoniae by gel filtration column chromatography. A crude extract of S. pneumoniae was prepared, untreated (A) or treated with RNase A (1 mg/ml) (B), and subjected to chromatography on a gel filtration column as described in Materials and Methods. The presence of Era in the fractions collected was detected by Western blotting analysis with polyclonal antibodies prepared against the native Era protein of S. pneumoniae ). The intensity of each band was quantified by scanning as described in Materials and Methods. Lanes 1 and 11, molecular mass markers and purified Era of S. pneumoniae (30 ng), respectively; lanes 2 to 10, fractions 23 to 31, respectively; lanes 12 to 19, fractions 39 to 46, respectively.

    Article Snippet: The peak fractions containing Era or RNA were treated with RNase A (Sigma) or DNase I (Gibco BRL) as indicated in the figure legends.

    Techniques: Filtration, Column Chromatography, Chromatography, Western Blot, Purification

    Effects of RNase A and DNase I treatments on the GST-Era GTPase activity of S. pneumoniae . Purified GST-Era protein (200 μg/ml) was treated with RNase A (200 or 800 μg/ml) or DNase I (100 μg/ml) at 37°C for 30 min. The RNase A- or DNase I-treated and untreated (control) GST-Era proteins (10 μg each) were then tested for their GTPase activities at 37°C for 30 min by the HPLC method as described in Materials and Methods. After the RNase A and DNase I treatments, parts of the GST-Era preparations were also analyzed by agarose gel electrophoresis (see Materials and Methods), and RNA was not detectable by ethidium bromide staining (data not shown).

    Journal: Journal of Bacteriology

    Article Title: 16S rRNA Is Bound to Era of Streptococcus pneumoniae

    doi:

    Figure Lengend Snippet: Effects of RNase A and DNase I treatments on the GST-Era GTPase activity of S. pneumoniae . Purified GST-Era protein (200 μg/ml) was treated with RNase A (200 or 800 μg/ml) or DNase I (100 μg/ml) at 37°C for 30 min. The RNase A- or DNase I-treated and untreated (control) GST-Era proteins (10 μg each) were then tested for their GTPase activities at 37°C for 30 min by the HPLC method as described in Materials and Methods. After the RNase A and DNase I treatments, parts of the GST-Era preparations were also analyzed by agarose gel electrophoresis (see Materials and Methods), and RNA was not detectable by ethidium bromide staining (data not shown).

    Article Snippet: The peak fractions containing Era or RNA were treated with RNase A (Sigma) or DNase I (Gibco BRL) as indicated in the figure legends.

    Techniques: Activity Assay, Purification, High Performance Liquid Chromatography, Agarose Gel Electrophoresis, Staining

    Immunoelectron microscopic localization of RNA polymerase II. Mouse L929 cell nuclei were reacted with one of two antibodies prepared against RNA polymerase II. Antibodies used in a ). IgGs were prepared by ammonium sulfate precipitation and DE52 chromatography followed by a β-galactosidase affinity column to remove antibodies against the bacterial protein. A dilution of 1:40 was used. The antibodies used in b ) and were the generous gift of Dr. Michael Dahmus. They were purified by Affigel-blue (Bio-Rad) chromatography, concentrated by ultrafiltration, and used at a dilution of 1:40. In both cases, reaction sites were located with goat anti-rabbit colloidal gold. Bar: 1 μm.

    Journal: Gene Expression

    Article Title: Preferential distribution of active RNA polymerase II molecules in the nuclear periphery

    doi:

    Figure Lengend Snippet: Immunoelectron microscopic localization of RNA polymerase II. Mouse L929 cell nuclei were reacted with one of two antibodies prepared against RNA polymerase II. Antibodies used in a ). IgGs were prepared by ammonium sulfate precipitation and DE52 chromatography followed by a β-galactosidase affinity column to remove antibodies against the bacterial protein. A dilution of 1:40 was used. The antibodies used in b ) and were the generous gift of Dr. Michael Dahmus. They were purified by Affigel-blue (Bio-Rad) chromatography, concentrated by ultrafiltration, and used at a dilution of 1:40. In both cases, reaction sites were located with goat anti-rabbit colloidal gold. Bar: 1 μm.

    Article Snippet: RNA polymerase II was inhibited and destabilized in mouse L929 cells by incubation in the presence of 5 μg/ml α-amanitin (Sigma) for 4 hours prior to nuclear isolation ( ).

    Techniques: Chromatography, Affinity Column, Purification

    Immunolocalization of RNA polymerase II after α-amanitin treatment of cells. Mouse L929 cells were incubated in 5 μg/ml α-amanitin for 4 hours, and nuclei were released and incubated with anti-RNA polymerase II, followed by immunogold labeling as described in Methods. Bar: 1 μm.

    Journal: Gene Expression

    Article Title: Preferential distribution of active RNA polymerase II molecules in the nuclear periphery

    doi:

    Figure Lengend Snippet: Immunolocalization of RNA polymerase II after α-amanitin treatment of cells. Mouse L929 cells were incubated in 5 μg/ml α-amanitin for 4 hours, and nuclei were released and incubated with anti-RNA polymerase II, followed by immunogold labeling as described in Methods. Bar: 1 μm.

    Article Snippet: RNA polymerase II was inhibited and destabilized in mouse L929 cells by incubation in the presence of 5 μg/ml α-amanitin (Sigma) for 4 hours prior to nuclear isolation ( ).

    Techniques: Incubation, Labeling

    Quantitative RT-PCR for total and pull-down RNA fraction. Total cellular RNA was isolated from Neuro-2a cells treated with 0.1mM or 1mM H 2 O 2 for 30min. ARP-RNA (1μg of total ARP-derivatized RNA) was reverse transcribed with random hexamers (left

    Journal: Analytical biochemistry

    Article Title: Quantification of oxidized levels of specific RNA species using an Aldehyde Reactive Probe

    doi: 10.1016/j.ab.2011.05.038

    Figure Lengend Snippet: Quantitative RT-PCR for total and pull-down RNA fraction. Total cellular RNA was isolated from Neuro-2a cells treated with 0.1mM or 1mM H 2 O 2 for 30min. ARP-RNA (1μg of total ARP-derivatized RNA) was reverse transcribed with random hexamers (left

    Article Snippet: The beads were suspended with Solution A (1M NaCl, 20mM Tris-HCl (pH7.5), 5mM EDTA, 1% NP-40) and incubated with 0.5mg/ml of E. coli transfer RNA (Sigma-Aldrich) for 30min at room temperature.

    Techniques: Quantitative RT-PCR, Isolation

    Assessment of pull-down using total cellular RNA. Total cellular RNA was isolated from HeLa cells treated with 0.1mM or 1mM H 2 O 2 for 30min. The isolated RNA was examined using ARP reactivity (A) and RNA pulled-down using strep-beads. The yield of RNA

    Journal: Analytical biochemistry

    Article Title: Quantification of oxidized levels of specific RNA species using an Aldehyde Reactive Probe

    doi: 10.1016/j.ab.2011.05.038

    Figure Lengend Snippet: Assessment of pull-down using total cellular RNA. Total cellular RNA was isolated from HeLa cells treated with 0.1mM or 1mM H 2 O 2 for 30min. The isolated RNA was examined using ARP reactivity (A) and RNA pulled-down using strep-beads. The yield of RNA

    Article Snippet: The beads were suspended with Solution A (1M NaCl, 20mM Tris-HCl (pH7.5), 5mM EDTA, 1% NP-40) and incubated with 0.5mg/ml of E. coli transfer RNA (Sigma-Aldrich) for 30min at room temperature.

    Techniques: Isolation

    Comparison of different storage temperature and trehalose for FTA Elute card stored for 2 days. ( A ) Untreated or 50 mg/ml trehalose-treated 6 mm FTA Elute card disc punch-out spotted with 20 µl serum were dried and stored at RT, 4 °C or −20 °C for 2 days before RNA was extracted using QIAzol lysis reagent. 10 miRNAs were quantified by RT-qPCR and their copy numbers were presented. Neat refers to copy number of each individual miRNA obtained from 20 µl serum extracted directly using QIAzol lysis reagent. The individual % miRNA recovery was compared between untreated and trehalose-treated FTA Elute card disc punch-out. ( B ) Average % miRNA recovery of all 10 miRNAs was calculated and plotted to compare the effect of pre-treatment of FTA Elute card disc punch-out with 50mg/ml trehalose. Statistical analyses were performed with one-way ANOVA, followed by Bonferroni’s pairwise comparisons test data between selected pairs. Each experimental condition was carried out thrice and data were presented as mean ± SEM (*** P ≤ 0.001; **P ≤ 0.01; * P ≤ 0.05, n = 3).

    Journal: Scientific Reports

    Article Title: Trehalose significantly enhances the recovery of serum and serum exosomal miRNA from a paper-based matrix

    doi: 10.1038/s41598-017-16960-8

    Figure Lengend Snippet: Comparison of different storage temperature and trehalose for FTA Elute card stored for 2 days. ( A ) Untreated or 50 mg/ml trehalose-treated 6 mm FTA Elute card disc punch-out spotted with 20 µl serum were dried and stored at RT, 4 °C or −20 °C for 2 days before RNA was extracted using QIAzol lysis reagent. 10 miRNAs were quantified by RT-qPCR and their copy numbers were presented. Neat refers to copy number of each individual miRNA obtained from 20 µl serum extracted directly using QIAzol lysis reagent. The individual % miRNA recovery was compared between untreated and trehalose-treated FTA Elute card disc punch-out. ( B ) Average % miRNA recovery of all 10 miRNAs was calculated and plotted to compare the effect of pre-treatment of FTA Elute card disc punch-out with 50mg/ml trehalose. Statistical analyses were performed with one-way ANOVA, followed by Bonferroni’s pairwise comparisons test data between selected pairs. Each experimental condition was carried out thrice and data were presented as mean ± SEM (*** P ≤ 0.001; **P ≤ 0.01; * P ≤ 0.05, n = 3).

    Article Snippet: To address this, we compared the performance of QIAzol lysis reagent with Trizol-LS reagent, Trizol reagent and column-based RNA isolation kit (Ambion).

    Techniques: Lysis, Quantitative RT-PCR

    miRNA expression level in GC patient and paired controls. ( A ) 20 µl of each of the 10 GC patients and 10 paired control serum were spotted on individual 6 mm FTA Elute card disc punch-out. 8 miRNAs were quantified and miRNA recovery was compared with the sample in which RNA was isolated directly from 20 µl serum using QIAzol lysis reagent (neat). ( B ) Correlation of miRNA representation from neat serum or FTA Elute card discs. Each experimental condition consisted of 10 biological replicates and data were presented as mean ± SEM. Statistical analyses were performed with one-way ANOVA, followed by Bonferroni’s pairwise comparisons test data for selected pairs (*** P ≤ 0.001; ** P ≤ 0.01; * P ≤ 0.05; ns: not significant).

    Journal: Scientific Reports

    Article Title: Trehalose significantly enhances the recovery of serum and serum exosomal miRNA from a paper-based matrix

    doi: 10.1038/s41598-017-16960-8

    Figure Lengend Snippet: miRNA expression level in GC patient and paired controls. ( A ) 20 µl of each of the 10 GC patients and 10 paired control serum were spotted on individual 6 mm FTA Elute card disc punch-out. 8 miRNAs were quantified and miRNA recovery was compared with the sample in which RNA was isolated directly from 20 µl serum using QIAzol lysis reagent (neat). ( B ) Correlation of miRNA representation from neat serum or FTA Elute card discs. Each experimental condition consisted of 10 biological replicates and data were presented as mean ± SEM. Statistical analyses were performed with one-way ANOVA, followed by Bonferroni’s pairwise comparisons test data for selected pairs (*** P ≤ 0.001; ** P ≤ 0.01; * P ≤ 0.05; ns: not significant).

    Article Snippet: To address this, we compared the performance of QIAzol lysis reagent with Trizol-LS reagent, Trizol reagent and column-based RNA isolation kit (Ambion).

    Techniques: Expressing, Isolation, Lysis

    Effect on miRNA yield during long-term FTA Elute card storage. ( A ) 20 µl serum spotted on trehalose-treated 6 mm FTA Elute card disc punch-out was subjected to accelerated aging testing of 1, 6 and 12 months before RNA was extracted using QIAzol lysis reagent. 10 miRNAs were quantified. The copy number of each miRNA was compared against the copy number obtained after storage at RT for 2 days. ( B ) Average % miRNA recovery of all 10 miRNAs for accelerated aging of 1, 6, 12 months was determined and compared with 2 days. Statistical analyses were performed with one-way ANOVA, followed by Bonferroni’s pairwise comparisons test data. Each experimental condition was carried out thrice and data were presented as mean ± SEM (*** P ≤ 0.001; **P ≤ 0.01; * P ≤ 0.05; ns: not significant, n = 3).

    Journal: Scientific Reports

    Article Title: Trehalose significantly enhances the recovery of serum and serum exosomal miRNA from a paper-based matrix

    doi: 10.1038/s41598-017-16960-8

    Figure Lengend Snippet: Effect on miRNA yield during long-term FTA Elute card storage. ( A ) 20 µl serum spotted on trehalose-treated 6 mm FTA Elute card disc punch-out was subjected to accelerated aging testing of 1, 6 and 12 months before RNA was extracted using QIAzol lysis reagent. 10 miRNAs were quantified. The copy number of each miRNA was compared against the copy number obtained after storage at RT for 2 days. ( B ) Average % miRNA recovery of all 10 miRNAs for accelerated aging of 1, 6, 12 months was determined and compared with 2 days. Statistical analyses were performed with one-way ANOVA, followed by Bonferroni’s pairwise comparisons test data. Each experimental condition was carried out thrice and data were presented as mean ± SEM (*** P ≤ 0.001; **P ≤ 0.01; * P ≤ 0.05; ns: not significant, n = 3).

    Article Snippet: To address this, we compared the performance of QIAzol lysis reagent with Trizol-LS reagent, Trizol reagent and column-based RNA isolation kit (Ambion).

    Techniques: Lysis

    miRNA recovery from serum-spotted FTA Elute cards using water and heat was inefficient. ( A ) RNA was extracted directly from 20 µl serum (black) or 20 µl plasma collected in EDTA-containing tube (plasma-EDTA) (white) using QIAzol lysis reagent. 10 miRNAs were quantified by RT-qPCR based on their copy numbers. RNA was extracted from a 6 mm FTA Elute card disc punch-out spotted with 20 µl serum (black) or 20 µl plasma-EDTA (white), using water and heating at 95 °C for 30 min. 10 miRNAs were quantified by RT-qPCR and their copy numbers ( B ) and % miRNA recovery ( C ) were presented. (% recovery = (Copy number of miRNA extracted from FTA Elute card disc punch-out/Copy number of miRNA extracted directly from serum) × 100%). Each experimental condition was carried out thrice and data were presented as mean ± SEM (*** P ≤ 0.001; ** P ≤ 0.01; * P ≤ 0.05; ns: not significant, Student’s t -test, n = 3).

    Journal: Scientific Reports

    Article Title: Trehalose significantly enhances the recovery of serum and serum exosomal miRNA from a paper-based matrix

    doi: 10.1038/s41598-017-16960-8

    Figure Lengend Snippet: miRNA recovery from serum-spotted FTA Elute cards using water and heat was inefficient. ( A ) RNA was extracted directly from 20 µl serum (black) or 20 µl plasma collected in EDTA-containing tube (plasma-EDTA) (white) using QIAzol lysis reagent. 10 miRNAs were quantified by RT-qPCR based on their copy numbers. RNA was extracted from a 6 mm FTA Elute card disc punch-out spotted with 20 µl serum (black) or 20 µl plasma-EDTA (white), using water and heating at 95 °C for 30 min. 10 miRNAs were quantified by RT-qPCR and their copy numbers ( B ) and % miRNA recovery ( C ) were presented. (% recovery = (Copy number of miRNA extracted from FTA Elute card disc punch-out/Copy number of miRNA extracted directly from serum) × 100%). Each experimental condition was carried out thrice and data were presented as mean ± SEM (*** P ≤ 0.001; ** P ≤ 0.01; * P ≤ 0.05; ns: not significant, Student’s t -test, n = 3).

    Article Snippet: To address this, we compared the performance of QIAzol lysis reagent with Trizol-LS reagent, Trizol reagent and column-based RNA isolation kit (Ambion).

    Techniques: Lysis, Quantitative RT-PCR