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    Worthington Biochemical rna isolation
    Rna Isolation, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna isolation/product/Worthington Biochemical
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    99
    Millipore ribonucleic acid rna isolation
    Comparison of growth, gene transcription, translation and isobutanol production in engineered strains Syn-pEEK2, pEEK2- kivd , Syn-IB-7, -8, -9 and -6. A) <t>RT-PCR,</t> SDS-PAGE and Strep-tag Western-immunoblot (top to bottom). Each lane represents the results from a single engineered strain. –RT is a negative RT-PCR control using <t>RNA</t> as template without addition of RT enzyme to control for possible DNA contamination. The positive control is an RT-PCR carried out using the corresponding plasmid as template. Red arrows indicate the location of kivd , blue arrows indicate the expected location for different AHDs. The first row of the Strep-tag Western-immunoblot shows the expression of kivd and the second row shows the expression of all the ADHs. The negative control for protein gel and Western-immunoblot is the empty vector strain Syn-pEEK2. B) Growth curve during 7 days of cultivation. C) Isobutanol production at day 3, day 5 and day 6 from all the engineered strains. Results are the mean of 4 biological replicates and 3 technical replicates, error bars represent standard deviation. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
    Ribonucleic Acid Rna Isolation, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1200 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore rna isolation reagent
    Comparison of growth, gene transcription, translation and isobutanol production in engineered strains Syn-pEEK2, pEEK2- kivd , Syn-IB-7, -8, -9 and -6. A) <t>RT-PCR,</t> SDS-PAGE and Strep-tag Western-immunoblot (top to bottom). Each lane represents the results from a single engineered strain. –RT is a negative RT-PCR control using <t>RNA</t> as template without addition of RT enzyme to control for possible DNA contamination. The positive control is an RT-PCR carried out using the corresponding plasmid as template. Red arrows indicate the location of kivd , blue arrows indicate the expected location for different AHDs. The first row of the Strep-tag Western-immunoblot shows the expression of kivd and the second row shows the expression of all the ADHs. The negative control for protein gel and Western-immunoblot is the empty vector strain Syn-pEEK2. B) Growth curve during 7 days of cultivation. C) Isobutanol production at day 3, day 5 and day 6 from all the engineered strains. Results are the mean of 4 biological replicates and 3 technical replicates, error bars represent standard deviation. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
    Rna Isolation Reagent, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna isolation reagent/product/Millipore
    Average 99 stars, based on 75 article reviews
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    rna isolation reagent - by Bioz Stars, 2020-05
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    97
    Millipore rna isolation cells
    Knockdown of Cox6a2 by <t>RNA</t> interference reveals regulation of ATP levels. siRNAs were designed to target Cox6a2 and transfected into murine <t>C2C12</t> muscle cells in vitro. Relative mRNA expression of Cox6a2 was measured by qPCR 48 hours after transfection. Expression was normalized to Hprt1 and Ppia internal controls. (A) Expression of Cox6a2 was significantly reduced relative to its expression in control cells at 48 hours post-transfection of siRNA. (B) ATP levels and (C) ADP/ATP ratio were measured at D0, D4, and D8. All values are presented as mean ± SEM (n = 3).
    Rna Isolation Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna isolation cells/product/Millipore
    Average 97 stars, based on 55 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    Comparison of growth, gene transcription, translation and isobutanol production in engineered strains Syn-pEEK2, pEEK2- kivd , Syn-IB-7, -8, -9 and -6. A) RT-PCR, SDS-PAGE and Strep-tag Western-immunoblot (top to bottom). Each lane represents the results from a single engineered strain. –RT is a negative RT-PCR control using RNA as template without addition of RT enzyme to control for possible DNA contamination. The positive control is an RT-PCR carried out using the corresponding plasmid as template. Red arrows indicate the location of kivd , blue arrows indicate the expected location for different AHDs. The first row of the Strep-tag Western-immunoblot shows the expression of kivd and the second row shows the expression of all the ADHs. The negative control for protein gel and Western-immunoblot is the empty vector strain Syn-pEEK2. B) Growth curve during 7 days of cultivation. C) Isobutanol production at day 3, day 5 and day 6 from all the engineered strains. Results are the mean of 4 biological replicates and 3 technical replicates, error bars represent standard deviation. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Journal: Metabolic Engineering Communications

    Article Title: Isobutanol production in Synechocystis PCC 6803 using heterologous and endogenous alcohol dehydrogenases

    doi: 10.1016/j.meteno.2017.07.003

    Figure Lengend Snippet: Comparison of growth, gene transcription, translation and isobutanol production in engineered strains Syn-pEEK2, pEEK2- kivd , Syn-IB-7, -8, -9 and -6. A) RT-PCR, SDS-PAGE and Strep-tag Western-immunoblot (top to bottom). Each lane represents the results from a single engineered strain. –RT is a negative RT-PCR control using RNA as template without addition of RT enzyme to control for possible DNA contamination. The positive control is an RT-PCR carried out using the corresponding plasmid as template. Red arrows indicate the location of kivd , blue arrows indicate the expected location for different AHDs. The first row of the Strep-tag Western-immunoblot shows the expression of kivd and the second row shows the expression of all the ADHs. The negative control for protein gel and Western-immunoblot is the empty vector strain Syn-pEEK2. B) Growth curve during 7 days of cultivation. C) Isobutanol production at day 3, day 5 and day 6 from all the engineered strains. Results are the mean of 4 biological replicates and 3 technical replicates, error bars represent standard deviation. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: 2.6 RNA isolation and semi-quantitative reverse transcript PCR (RT-PCR) Total RNA was isolated from cultures (OD750 = 0.5) using RTI Reagent (Sigma-Aldrich) according to the manufacturer's instructions.

    Techniques: Reverse Transcription Polymerase Chain Reaction, SDS Page, Strep-tag, Western Blot, Positive Control, Plasmid Preparation, Expressing, Negative Control, Standard Deviation

    Comparison of isobutanol production in engineered Synechocystis PCC 6803 strains Syn-IB-1, -2, -3, -4, -5, and -6. A) RT-PCR, SDS-PAGE and Strep-tag Western-immunoblot (top to bottom). Every dashed line separated each lane represents the results from a single engineered strain. –RT is a negative RT-PCR control using RNA as template without addition of RT enzyme to control for possible DNA contamination. The positive control is an RT-PCR carried out using the corresponding plasmid as template. Red arrows in the SDS-PAGE indicate the location of kivd , blue arrows indicate the expected location for slr1192 . The Strep-tag Western-immunoblot examines the expression of all the ADHs. The negative control for SDS-PAGE and Western-immunoblot is an extract from strain Syn-pDDH. B) Growth curve during 7 days of cultivation. C) Isobutanol production at day 3, day 5 and day 6 from the different engineered strains. Results are the mean of 4 biological replicates and 3 technical replicates, error bars represent standard deviation. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Journal: Metabolic Engineering Communications

    Article Title: Isobutanol production in Synechocystis PCC 6803 using heterologous and endogenous alcohol dehydrogenases

    doi: 10.1016/j.meteno.2017.07.003

    Figure Lengend Snippet: Comparison of isobutanol production in engineered Synechocystis PCC 6803 strains Syn-IB-1, -2, -3, -4, -5, and -6. A) RT-PCR, SDS-PAGE and Strep-tag Western-immunoblot (top to bottom). Every dashed line separated each lane represents the results from a single engineered strain. –RT is a negative RT-PCR control using RNA as template without addition of RT enzyme to control for possible DNA contamination. The positive control is an RT-PCR carried out using the corresponding plasmid as template. Red arrows in the SDS-PAGE indicate the location of kivd , blue arrows indicate the expected location for slr1192 . The Strep-tag Western-immunoblot examines the expression of all the ADHs. The negative control for SDS-PAGE and Western-immunoblot is an extract from strain Syn-pDDH. B) Growth curve during 7 days of cultivation. C) Isobutanol production at day 3, day 5 and day 6 from the different engineered strains. Results are the mean of 4 biological replicates and 3 technical replicates, error bars represent standard deviation. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: 2.6 RNA isolation and semi-quantitative reverse transcript PCR (RT-PCR) Total RNA was isolated from cultures (OD750 = 0.5) using RTI Reagent (Sigma-Aldrich) according to the manufacturer's instructions.

    Techniques: Periodic Counter-current Chromatography, Reverse Transcription Polymerase Chain Reaction, SDS Page, Strep-tag, Western Blot, Positive Control, Plasmid Preparation, Expressing, Negative Control, Standard Deviation

    Expression of ERVWE1 and ERVW2 in chondrocytes from OA and non- or early OA patients. (a) PCR on cDNA prepared from cultured chondrocytes isolated from knees or hip from OA patients. Specific primers for the ERVWE1 and ERVWE2 env gene (encoding syncytin) were used. (b) RNA purified from nitrogen-crushed cartilage. (c) RNA was isolated from chondrocytes obtained from 6 OA patients, converted into cDNA, and amplified using specific primers complementary to the gag gene of HERVWE1 (left panel) and HERVWE2 (right panel) sequences. (d) Amplification of cDNA prepared from chondrocytes from non- and early OA patients. Lanes 1 and 11: 1 kb plus ladder; lanes 2–10: amplified cDNA. The arrow indicates the presence of the amplified 3,000 bp of the ERVWE1 env transcript.

    Journal: BioMed Research International

    Article Title: Human Endogenous Retrovirus W Activity in Cartilage of Osteoarthritis Patients

    doi: 10.1155/2014/698609

    Figure Lengend Snippet: Expression of ERVWE1 and ERVW2 in chondrocytes from OA and non- or early OA patients. (a) PCR on cDNA prepared from cultured chondrocytes isolated from knees or hip from OA patients. Specific primers for the ERVWE1 and ERVWE2 env gene (encoding syncytin) were used. (b) RNA purified from nitrogen-crushed cartilage. (c) RNA was isolated from chondrocytes obtained from 6 OA patients, converted into cDNA, and amplified using specific primers complementary to the gag gene of HERVWE1 (left panel) and HERVWE2 (right panel) sequences. (d) Amplification of cDNA prepared from chondrocytes from non- and early OA patients. Lanes 1 and 11: 1 kb plus ladder; lanes 2–10: amplified cDNA. The arrow indicates the presence of the amplified 3,000 bp of the ERVWE1 env transcript.

    Article Snippet: RNA-Isolating and cDNA Synthesis Medium was removed and cells were washed in PBS; then PBS was removed and the cell pellet was quickly frozen at −2°C, ready for RNA-isolating or resolved in freezing medium (medium with 20% bovine serum and 10% DMSO (Sigma-Aldrich)).

    Techniques: Expressing, Polymerase Chain Reaction, Cell Culture, Isolation, Purification, Amplification

    Detection of human endogenous retrovirus sequences in chondrocytes and cartilage from OA patients. Total RNA was isolated and converted into cDNA. HERV sequences were amplified using degenerated primers complementary to sequences in the pro/pol genes of HRV-5 ( Table 1 ) to obtain a ~1,000 bp fragment. Lane 1: DNA marker (in kb); lanes 2–4: OA8; lanes 4, 8, and 10: OA10; lanes 6–8: OA9; lane 11: OA2. Lanes 2, 8, and 9: RNA isolated from nitrogen-crushed cartilage; lanes 3, 7, and 10: RNA obtained from enzyme-digested cartilage; lanes 4–6 and 11: RNA purified from culture-expanded chondrocytes. PCR reactions were run on an agarose gel, stained with ethidium bromide, and the DNA was visualized under UV light. Cart.: cartilage; chond.: chondrocytes.

    Journal: BioMed Research International

    Article Title: Human Endogenous Retrovirus W Activity in Cartilage of Osteoarthritis Patients

    doi: 10.1155/2014/698609

    Figure Lengend Snippet: Detection of human endogenous retrovirus sequences in chondrocytes and cartilage from OA patients. Total RNA was isolated and converted into cDNA. HERV sequences were amplified using degenerated primers complementary to sequences in the pro/pol genes of HRV-5 ( Table 1 ) to obtain a ~1,000 bp fragment. Lane 1: DNA marker (in kb); lanes 2–4: OA8; lanes 4, 8, and 10: OA10; lanes 6–8: OA9; lane 11: OA2. Lanes 2, 8, and 9: RNA isolated from nitrogen-crushed cartilage; lanes 3, 7, and 10: RNA obtained from enzyme-digested cartilage; lanes 4–6 and 11: RNA purified from culture-expanded chondrocytes. PCR reactions were run on an agarose gel, stained with ethidium bromide, and the DNA was visualized under UV light. Cart.: cartilage; chond.: chondrocytes.

    Article Snippet: RNA-Isolating and cDNA Synthesis Medium was removed and cells were washed in PBS; then PBS was removed and the cell pellet was quickly frozen at −2°C, ready for RNA-isolating or resolved in freezing medium (medium with 20% bovine serum and 10% DMSO (Sigma-Aldrich)).

    Techniques: Isolation, Amplification, Marker, Purification, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining

    Differential expression of LdCLrP in clinical isolates. (A) Expression analysis of CLrP in different clinical isolates by qRT-PCR. The data are presented as the mean ± SD of three independent RNA preparations. (Asterisks denote highly significant differences from S1) (B) Differential expression pattern of CLrP in different isolates by Western blot of WCL of L . donovani . Actin served as an internal loading control (C) Fold expression of CLrP in different clinical isolates (densitometric analysis of three different western blots with three independent sample preparations).

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Over-Expression of Cysteine Leucine Rich Protein Is Related to SAG Resistance in Clinical Isolates of Leishmania donovani

    doi: 10.1371/journal.pntd.0003992

    Figure Lengend Snippet: Differential expression of LdCLrP in clinical isolates. (A) Expression analysis of CLrP in different clinical isolates by qRT-PCR. The data are presented as the mean ± SD of three independent RNA preparations. (Asterisks denote highly significant differences from S1) (B) Differential expression pattern of CLrP in different isolates by Western blot of WCL of L . donovani . Actin served as an internal loading control (C) Fold expression of CLrP in different clinical isolates (densitometric analysis of three different western blots with three independent sample preparations).

    Article Snippet: Quantitative real time (qRT) and western analysis of CLrP expression in clinical isolates RNA of log phase promastigotes (S1, S2, R1, R2, R3) (107 parasites) was isolated using Tri reagent (Sigma, Aldrich) followed by DNase treatment and quantified. cDNA was synthesized using First-strand cDNA synthesis kit (Fermentas). qRT-PCR was carried out with 12.5μl of SYBR green PCR master mix (TAKARA), 1μg of cDNA, and 200nM primer (Table A in ) in a final volume of 25μl. qRT was performed with the following conditions: initial denaturation at 95°C for 10min x 40 cycles, each consisting of denaturation at 95°C for 1 min, annealing at 52°C for 1 min and extension at 70°C for 1min followed by 80°C for 10sec.

    Techniques: Expressing, Quantitative RT-PCR, Western Blot

    Venn diagram of durum wheat miRNAs and target genes identified in nitrogen deprivation conditions. Conserved and novel durum wheat miRNAs (A) and target genes (B) identified in four small RNA libraries, two from Ciccio and Svevo cultivars grown in nitrogen deprivation conditions (0mM nitrogen, this work), and two from the same cultivars grown in standard conditions (2mM nitrogen, De Paola et al., 2016); the number of miRNAs (A) or target genes (B) present in one or more libraries is indicated.

    Journal: PLoS ONE

    Article Title: Durum wheat miRNAs in response to nitrogen starvation at the grain filling stage

    doi: 10.1371/journal.pone.0183253

    Figure Lengend Snippet: Venn diagram of durum wheat miRNAs and target genes identified in nitrogen deprivation conditions. Conserved and novel durum wheat miRNAs (A) and target genes (B) identified in four small RNA libraries, two from Ciccio and Svevo cultivars grown in nitrogen deprivation conditions (0mM nitrogen, this work), and two from the same cultivars grown in standard conditions (2mM nitrogen, De Paola et al., 2016); the number of miRNAs (A) or target genes (B) present in one or more libraries is indicated.

    Article Snippet: Small RNA library preparation For small RNA (sRNA) isolation, mirPremier microRNA Isolation Kit (Sigma-Aldrich, St. Louis, MO, USA) was used with 100 mg of each tissue from each plant.

    Techniques:

    Knockdown of Cox6a2 by RNA interference reveals regulation of ATP levels. siRNAs were designed to target Cox6a2 and transfected into murine C2C12 muscle cells in vitro. Relative mRNA expression of Cox6a2 was measured by qPCR 48 hours after transfection. Expression was normalized to Hprt1 and Ppia internal controls. (A) Expression of Cox6a2 was significantly reduced relative to its expression in control cells at 48 hours post-transfection of siRNA. (B) ATP levels and (C) ADP/ATP ratio were measured at D0, D4, and D8. All values are presented as mean ± SEM (n = 3).

    Journal: PLoS ONE

    Article Title: MicroRNAs Regulate Cellular ATP Levels by Targeting Mitochondrial Energy Metabolism Genes during C2C12 Myoblast Differentiation

    doi: 10.1371/journal.pone.0127850

    Figure Lengend Snippet: Knockdown of Cox6a2 by RNA interference reveals regulation of ATP levels. siRNAs were designed to target Cox6a2 and transfected into murine C2C12 muscle cells in vitro. Relative mRNA expression of Cox6a2 was measured by qPCR 48 hours after transfection. Expression was normalized to Hprt1 and Ppia internal controls. (A) Expression of Cox6a2 was significantly reduced relative to its expression in control cells at 48 hours post-transfection of siRNA. (B) ATP levels and (C) ADP/ATP ratio were measured at D0, D4, and D8. All values are presented as mean ± SEM (n = 3).

    Article Snippet: RNA isolation Total RNA was extracted from cultured C2C12 cells using Tri-Reagent (Sigma-Aldrich, Germany) followed by an on-column DNase treatment.

    Techniques: Transfection, In Vitro, Expressing, Real-time Polymerase Chain Reaction

    Molecular analysis of in planta T 1 transgenic horse gram plants. a , b Genomic DNA was isolated from control (CN) and nine putative T 1 transgenic horse gram leaf samples and the PCR analysis performed using NPT II ( a ) and GUS ( b ) gene-specific primers, respectively. c , d RT-PCR analysis of transgenic horse gram plants using NPT II c and GUS d gene-specific primers, respectively. Total RNA was isolated from control and putative transgenic lines and then converted to the single-stranded cDNA and performed the RT-PCR to study the expression of NPT II and GUS transcript. Note: 796 bp and 1.0 kb PCR products were observed in all transgenic plants except control (CN) using NPT II and GUS gene-specific primer, respectively. M 1.0 kb DNA ladder (Fermentas, Thermo Scientific), CN control plant, H1–H9 different transgenic lines, BP E. coli binary plasmid pCAMBIA2301

    Journal: Journal of Genetic Engineering & Biotechnology

    Article Title: A simple and efficient Agrobacterium-mediated in planta transformation protocol for horse gram (Macrotyloma uniflorum Lam. Verdc.)

    doi: 10.1186/s43141-020-00023-z

    Figure Lengend Snippet: Molecular analysis of in planta T 1 transgenic horse gram plants. a , b Genomic DNA was isolated from control (CN) and nine putative T 1 transgenic horse gram leaf samples and the PCR analysis performed using NPT II ( a ) and GUS ( b ) gene-specific primers, respectively. c , d RT-PCR analysis of transgenic horse gram plants using NPT II c and GUS d gene-specific primers, respectively. Total RNA was isolated from control and putative transgenic lines and then converted to the single-stranded cDNA and performed the RT-PCR to study the expression of NPT II and GUS transcript. Note: 796 bp and 1.0 kb PCR products were observed in all transgenic plants except control (CN) using NPT II and GUS gene-specific primer, respectively. M 1.0 kb DNA ladder (Fermentas, Thermo Scientific), CN control plant, H1–H9 different transgenic lines, BP E. coli binary plasmid pCAMBIA2301

    Article Snippet: Total RNA isolation and semi-quantitative RT-PCR analysis Total RNA was extracted from GUS and genomic PCR-positive transgenic and wild-type control plants by using spectrumTM plant total RNA isolation kit (Sigma-Aldrich, USA).

    Techniques: Transgenic Assay, Isolation, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Expressing, Plasmid Preparation