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  • 94
    Agilent technologies rna integrity number rin
    Impact of enrichment for viable leukemic blasts on <t>RNA</t> quality. Figure shows the positive impact of enrichment on the global quality of RNA. Specimens in the lowest and highest tertiles of viability by DAPI are included. Figure shows the correlation between viability (DAPI, x -axis) and <t>RIN</t> ( y -axis) in unsorted MNCs (A) and enriched blasts (B) from bone marrow ( light gray circles ) and peripheral blood ( dark gray squares ). The majority of specimens with poor viability and low RIN numbers (i.e., suboptimal RNA quality) in unsorted MNCs demonstrated an improvement in RIN numbers after enrichment for viable AML blasts (RIN
    Rna Integrity Number Rin, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 5896 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Agilent technologies rna integrity number rin software
    (A) <t>RNA</t> integrity versus processing time (T p ): FNA-sample <t>RIN</t> scores do not correlate with lag time between aspiration and freezing time (T p ) when specimens are processed for cryopreservation within 1 hour of aspiration. (B) RNA integrity versus frozen time duration (T f ): FNA-sample RIN scores do not correlate with total frozen time (T f ).
    Rna Integrity Number Rin Software, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Agilent technologies bioanalyzer rna integrity number rin
    Linear regression analysis for <t>RNA</t> integrity compared to postmortem interval for heart, liver, lymph node and adrenal. Results show linear regression lines for each tissue for <t>RIN</t> compared to PMI with 95 % confidence limits. Results show Pearson r values
    Bioanalyzer Rna Integrity Number Rin, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Illumina Inc rna integrity number rin
    Bioanalyzer results for total <t>RNA</t> isolated from (A) AS-FFPE HCC tissues, and (B) good, intermediate, and poor quality FC-FFPE tissues. The AS- and FC-FFPE RNA samples were extracted from different samples. AS: archived section, FFPE: formalin-fixed paraffin-embedded, HCC: hepatocellular carcinoma, FC: freshly cut, <t>RIN:</t> RNA Integrity Number.
    Rna Integrity Number Rin, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 661 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher rna integrity number rin
    Bioanalyzer results for total <t>RNA</t> isolated from (A) AS-FFPE HCC tissues, and (B) good, intermediate, and poor quality FC-FFPE tissues. The AS- and FC-FFPE RNA samples were extracted from different samples. AS: archived section, FFPE: formalin-fixed paraffin-embedded, HCC: hepatocellular carcinoma, FC: freshly cut, <t>RIN:</t> RNA Integrity Number.
    Rna Integrity Number Rin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 759 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Avantor rna integrity number rin
    Bioanalyzer results for total <t>RNA</t> isolated from (A) AS-FFPE HCC tissues, and (B) good, intermediate, and poor quality FC-FFPE tissues. The AS- and FC-FFPE RNA samples were extracted from different samples. AS: archived section, FFPE: formalin-fixed paraffin-embedded, HCC: hepatocellular carcinoma, FC: freshly cut, <t>RIN:</t> RNA Integrity Number.
    Rna Integrity Number Rin, supplied by Avantor, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Novogene rna integrity number rins
    Bioanalyzer results for total <t>RNA</t> isolated from (A) AS-FFPE HCC tissues, and (B) good, intermediate, and poor quality FC-FFPE tissues. The AS- and FC-FFPE RNA samples were extracted from different samples. AS: archived section, FFPE: formalin-fixed paraffin-embedded, HCC: hepatocellular carcinoma, FC: freshly cut, <t>RIN:</t> RNA Integrity Number.
    Rna Integrity Number Rins, supplied by Novogene, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher 1000ănd rna integrity number rin
    Bioanalyzer results for total <t>RNA</t> isolated from (A) AS-FFPE HCC tissues, and (B) good, intermediate, and poor quality FC-FFPE tissues. The AS- and FC-FFPE RNA samples were extracted from different samples. AS: archived section, FFPE: formalin-fixed paraffin-embedded, HCC: hepatocellular carcinoma, FC: freshly cut, <t>RIN:</t> RNA Integrity Number.
    1000ănd Rna Integrity Number Rin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Impact of enrichment for viable leukemic blasts on RNA quality. Figure shows the positive impact of enrichment on the global quality of RNA. Specimens in the lowest and highest tertiles of viability by DAPI are included. Figure shows the correlation between viability (DAPI, x -axis) and RIN ( y -axis) in unsorted MNCs (A) and enriched blasts (B) from bone marrow ( light gray circles ) and peripheral blood ( dark gray squares ). The majority of specimens with poor viability and low RIN numbers (i.e., suboptimal RNA quality) in unsorted MNCs demonstrated an improvement in RIN numbers after enrichment for viable AML blasts (RIN

    Journal: Biopreservation and Biobanking

    Article Title: Impact of Specimen Heterogeneity on Biomarkers in Repository Samples from Patients with Acute Myeloid Leukemia: A SWOG Report

    doi: 10.1089/bio.2017.0079

    Figure Lengend Snippet: Impact of enrichment for viable leukemic blasts on RNA quality. Figure shows the positive impact of enrichment on the global quality of RNA. Specimens in the lowest and highest tertiles of viability by DAPI are included. Figure shows the correlation between viability (DAPI, x -axis) and RIN ( y -axis) in unsorted MNCs (A) and enriched blasts (B) from bone marrow ( light gray circles ) and peripheral blood ( dark gray squares ). The majority of specimens with poor viability and low RIN numbers (i.e., suboptimal RNA quality) in unsorted MNCs demonstrated an improvement in RIN numbers after enrichment for viable AML blasts (RIN

    Article Snippet: RNA integrity number (RIN) was determined on Agilent 2200 TapeStation (Agilent Technologies, Santa Clara, CA).

    Techniques:

    Impact of histological stain and fixative/protector on morphology of goat mammary tissue and RNA integrity . Frozen mammary tissue sections were stained either with HistoGene ® (A to C) or with Cresyl violet ® (D and E). MEC isolated after treatment with RCL2, a new fixative preserving tissue morphology and Nucleic Acid integrity (C and D), or with RNA later (B) provide RNA of better quality, expressed as ΔRIN which is difference between the RIN value obtained with total RNA extracted from the mammary tissue section (8.5) and the RIN value obtained with RNA extracted from microdissected cells.

    Journal: BMC Cell Biology

    Article Title: Maintaining RNA integrity in a homogeneous population of mammary epithelial cells isolated by Laser Capture Microdissection

    doi: 10.1186/1471-2121-11-95

    Figure Lengend Snippet: Impact of histological stain and fixative/protector on morphology of goat mammary tissue and RNA integrity . Frozen mammary tissue sections were stained either with HistoGene ® (A to C) or with Cresyl violet ® (D and E). MEC isolated after treatment with RCL2, a new fixative preserving tissue morphology and Nucleic Acid integrity (C and D), or with RNA later (B) provide RNA of better quality, expressed as ΔRIN which is difference between the RIN value obtained with total RNA extracted from the mammary tissue section (8.5) and the RIN value obtained with RNA extracted from microdissected cells.

    Article Snippet: Quality was evaluated using the RNA Integrity Number (RIN) value introduced by Agilent [ ].

    Techniques: Staining, Isolation, Preserving

    Effects of delayed dissections on RNA quality in spleens and livers. Spleens were collected at 1 (Positive Control), 20, 40, 60, 80, and 100 minutes post-euthanasia, and were preserved in RNAlater. RNAlater-preserved spleen samples yielded high RIN values (RIN > 7.5) for all time points (A). Liver tissues from the same mice in (A) were collected at 2 (Positive Control), 25, 45, 65, 85, and 105 minutes post-euthanasia, and were frozen on dry ice. RNA quality in samples collected up to 45 minutes post-euthanasia were minimally affected, resulting in RIN values > 8. RIN value decreased by 24% in samples collected at 105 minutes post-euthanasia, compared to controls (B). All samples were stored at -80°C for 1 month prior to analysis. Data sets were assessed for normality using the Shapiro-Wilk test, followed by the Dunn post-hoc test (Joint Ranks, compared to Control). Values shown are medians within interquartile (boxes) and full range (whiskers). (n = 4 for each time point). * p

    Journal: PLoS ONE

    Article Title: Preservation of Multiple Mammalian Tissues to Maximize Science Return from Ground Based and Spaceflight Experiments

    doi: 10.1371/journal.pone.0167391

    Figure Lengend Snippet: Effects of delayed dissections on RNA quality in spleens and livers. Spleens were collected at 1 (Positive Control), 20, 40, 60, 80, and 100 minutes post-euthanasia, and were preserved in RNAlater. RNAlater-preserved spleen samples yielded high RIN values (RIN > 7.5) for all time points (A). Liver tissues from the same mice in (A) were collected at 2 (Positive Control), 25, 45, 65, 85, and 105 minutes post-euthanasia, and were frozen on dry ice. RNA quality in samples collected up to 45 minutes post-euthanasia were minimally affected, resulting in RIN values > 8. RIN value decreased by 24% in samples collected at 105 minutes post-euthanasia, compared to controls (B). All samples were stored at -80°C for 1 month prior to analysis. Data sets were assessed for normality using the Shapiro-Wilk test, followed by the Dunn post-hoc test (Joint Ranks, compared to Control). Values shown are medians within interquartile (boxes) and full range (whiskers). (n = 4 for each time point). * p

    Article Snippet: RNA quantity and quality was assessed by measuring RNA Integrity Number (RIN) values using an Agilent Bioanalyzer.

    Techniques: Positive Control, Mouse Assay

    Effects of preservation times post-euthanasia on RNA quality in spleen and liver. Spleens and livers were collected from up to 3 minutes (Positive Control), 9 minutes (Set 1), and 25 minutes (Set 2) post-euthanasia. Set 1 and 2 time points were selected to simulate anticipated dissection timing in microgravity. Spleens were preserved in RNAlater, and livers were frozen on dry ice. All samples were stored at -80°C, and analyzed after 3.5 months for spleen and 4.5 months for liver. RNA quality was measured by calculating the Bioanalyzer-based RNA integrity number (RIN) using the Bioanalyzer 2100. Samples harvested up to 25 minutes post-euthanasia, and stored for less than 5 months at -80°C, yielded RIN values greater than 8. Data sets were assessed for normality using the Shapiro-Wilk test, followed by the Kruskal-Wallis test. Values shown are medians within interquartile (boxes) and full range (whiskers). Spleens: n = 4, 9, and 8 for Control, Set 1 and 2, respectively. Livers: n = 3, 10, and 10 for Control, Set 1 and 2, respectively.

    Journal: PLoS ONE

    Article Title: Preservation of Multiple Mammalian Tissues to Maximize Science Return from Ground Based and Spaceflight Experiments

    doi: 10.1371/journal.pone.0167391

    Figure Lengend Snippet: Effects of preservation times post-euthanasia on RNA quality in spleen and liver. Spleens and livers were collected from up to 3 minutes (Positive Control), 9 minutes (Set 1), and 25 minutes (Set 2) post-euthanasia. Set 1 and 2 time points were selected to simulate anticipated dissection timing in microgravity. Spleens were preserved in RNAlater, and livers were frozen on dry ice. All samples were stored at -80°C, and analyzed after 3.5 months for spleen and 4.5 months for liver. RNA quality was measured by calculating the Bioanalyzer-based RNA integrity number (RIN) using the Bioanalyzer 2100. Samples harvested up to 25 minutes post-euthanasia, and stored for less than 5 months at -80°C, yielded RIN values greater than 8. Data sets were assessed for normality using the Shapiro-Wilk test, followed by the Kruskal-Wallis test. Values shown are medians within interquartile (boxes) and full range (whiskers). Spleens: n = 4, 9, and 8 for Control, Set 1 and 2, respectively. Livers: n = 3, 10, and 10 for Control, Set 1 and 2, respectively.

    Article Snippet: RNA quantity and quality was assessed by measuring RNA Integrity Number (RIN) values using an Agilent Bioanalyzer.

    Techniques: Preserving, Positive Control, Dissection

    The effects on RNA quality in various tissues recovered from whole and partially dissected carcasses stored at -80°C for 2.5 months. Tissue samples were removed from the frozen carcasses for RIN analysis. Tissues were preserved in RNAlater prior to analysis. Brain, adrenal glands, eye, thymus, and hindlimb muscles resulted in high RIN values in both WC (A) and PC (B). RNA quality in WC and PC small intestine and bone marrow samples were severely degraded, resulting in RIN values

    Journal: PLoS ONE

    Article Title: Preservation of Multiple Mammalian Tissues to Maximize Science Return from Ground Based and Spaceflight Experiments

    doi: 10.1371/journal.pone.0167391

    Figure Lengend Snippet: The effects on RNA quality in various tissues recovered from whole and partially dissected carcasses stored at -80°C for 2.5 months. Tissue samples were removed from the frozen carcasses for RIN analysis. Tissues were preserved in RNAlater prior to analysis. Brain, adrenal glands, eye, thymus, and hindlimb muscles resulted in high RIN values in both WC (A) and PC (B). RNA quality in WC and PC small intestine and bone marrow samples were severely degraded, resulting in RIN values

    Article Snippet: RNA quantity and quality was assessed by measuring RNA Integrity Number (RIN) values using an Agilent Bioanalyzer.

    Techniques:

    Effects of long duration storage at -80°C on RNA quality in spleen and liver. Spleens and livers were collected up to 5 minutes post-euthanasia. Spleens were preserved in RNAlater, and livers were frozen on dry ice. Samples were stored at -80°C for 1, 3.5, and 11 months for spleen, and 1, 4.5, and 11 months for liver. Samples stored up to 11 months at -80°C resulted in RIN values greater than 8. Data sets were assessed for normality using the Shapiro-Wilk test, followed by the Kruskal-Wallis test. Values shown are medians within interquartile (boxes) and full range (whiskers) (n = 4 for all months except for n = 3 for Liver 12 months). * p

    Journal: PLoS ONE

    Article Title: Preservation of Multiple Mammalian Tissues to Maximize Science Return from Ground Based and Spaceflight Experiments

    doi: 10.1371/journal.pone.0167391

    Figure Lengend Snippet: Effects of long duration storage at -80°C on RNA quality in spleen and liver. Spleens and livers were collected up to 5 minutes post-euthanasia. Spleens were preserved in RNAlater, and livers were frozen on dry ice. Samples were stored at -80°C for 1, 3.5, and 11 months for spleen, and 1, 4.5, and 11 months for liver. Samples stored up to 11 months at -80°C resulted in RIN values greater than 8. Data sets were assessed for normality using the Shapiro-Wilk test, followed by the Kruskal-Wallis test. Values shown are medians within interquartile (boxes) and full range (whiskers) (n = 4 for all months except for n = 3 for Liver 12 months). * p

    Article Snippet: RNA quantity and quality was assessed by measuring RNA Integrity Number (RIN) values using an Agilent Bioanalyzer.

    Techniques:

    Quality control of total RNA isolated from paired control and lyophilized samples stored for two weeks or two months at room temperature ( A ) RNA yield per million cells. Horizontal lines represent mean values (The P value for the two-week samples is 0.97, and that for the two-month samples is 0.16; paired t -test) ( B ) Calculated RIN values of paired samples. Horizontal lines indicate mean values ( P = 1.0 for the two-week samples, and P = 0.1 for the two-month samples; paired t -test). ( C ) The GAPDH mRNA 3′/5′ ratio of paired samples ( P = 0.86 for the two-week samples, and P = 0.92 for the two-month samples; paired t -test).

    Journal: Oncotarget

    Article Title: Lyophilized human cells stored at room temperature preserve multiple RNA species at excellent quality for RNA sequencing

    doi: 10.18632/oncotarget.25764

    Figure Lengend Snippet: Quality control of total RNA isolated from paired control and lyophilized samples stored for two weeks or two months at room temperature ( A ) RNA yield per million cells. Horizontal lines represent mean values (The P value for the two-week samples is 0.97, and that for the two-month samples is 0.16; paired t -test) ( B ) Calculated RIN values of paired samples. Horizontal lines indicate mean values ( P = 1.0 for the two-week samples, and P = 0.1 for the two-month samples; paired t -test). ( C ) The GAPDH mRNA 3′/5′ ratio of paired samples ( P = 0.86 for the two-week samples, and P = 0.92 for the two-month samples; paired t -test).

    Article Snippet: RIN (RNA integrity number) values were calculated from Agilent electropherograms for control samples as 10, while lyophilized samples also showed a remarkably high RIN value average of 9.8 (Figure ).

    Techniques: Isolation

    Quantity and quality of RNA isolated from paired control and lyophilized cells (immediately after lyophilization) ( A ) RNA yield per million cells. Horizontal lines represent mean values ( P = 0.68, paired t -test) ( B ) Calculated RIN values of paired samples; horizontal lines indicate mean values ( P = 0.15, paired t -test). ( C ) The GAPDH mRNA 3′/5′ ratio of paired samples ( P = 0.34, paired t -test).

    Journal: Oncotarget

    Article Title: Lyophilized human cells stored at room temperature preserve multiple RNA species at excellent quality for RNA sequencing

    doi: 10.18632/oncotarget.25764

    Figure Lengend Snippet: Quantity and quality of RNA isolated from paired control and lyophilized cells (immediately after lyophilization) ( A ) RNA yield per million cells. Horizontal lines represent mean values ( P = 0.68, paired t -test) ( B ) Calculated RIN values of paired samples; horizontal lines indicate mean values ( P = 0.15, paired t -test). ( C ) The GAPDH mRNA 3′/5′ ratio of paired samples ( P = 0.34, paired t -test).

    Article Snippet: RIN (RNA integrity number) values were calculated from Agilent electropherograms for control samples as 10, while lyophilized samples also showed a remarkably high RIN value average of 9.8 (Figure ).

    Techniques: Isolation

    (A) RNA integrity versus processing time (T p ): FNA-sample RIN scores do not correlate with lag time between aspiration and freezing time (T p ) when specimens are processed for cryopreservation within 1 hour of aspiration. (B) RNA integrity versus frozen time duration (T f ): FNA-sample RIN scores do not correlate with total frozen time (T f ).

    Journal: Cancer cytopathology

    Article Title: Preservation of Fine-Needle Aspiration Specimens for Future Use in RNA-Based Molecular Testing

    doi: 10.1002/cncy.20130

    Figure Lengend Snippet: (A) RNA integrity versus processing time (T p ): FNA-sample RIN scores do not correlate with lag time between aspiration and freezing time (T p ) when specimens are processed for cryopreservation within 1 hour of aspiration. (B) RNA integrity versus frozen time duration (T f ): FNA-sample RIN scores do not correlate with total frozen time (T f ).

    Article Snippet: RNA integrity and yield were evaluated by analysis on the Agilent 2100 bioanalyzer RNA 6000 Pico Chip kit using the RNA Integrity Number (RIN) software (Agilent Technologies, Santa Clara, Calif).

    Techniques:

    Linear regression analysis for RNA integrity compared to postmortem interval for heart, liver, lymph node and adrenal. Results show linear regression lines for each tissue for RIN compared to PMI with 95 % confidence limits. Results show Pearson r values

    Journal: Cell and tissue banking

    Article Title: Characterization of RNA isolated from eighteen different human tissues: results from a rapid human autopsy program

    doi: 10.1007/s10561-016-9555-8

    Figure Lengend Snippet: Linear regression analysis for RNA integrity compared to postmortem interval for heart, liver, lymph node and adrenal. Results show linear regression lines for each tissue for RIN compared to PMI with 95 % confidence limits. Results show Pearson r values

    Article Snippet: The Agilent Bioanalyzer RNA integrity number (RIN) has become the accepted standard for assessing the intactness of isolated RNA from all sources ( ).

    Techniques:

    Effect of RIN on expression values of housekeeping normalization genes in RNA samples derived from heart. The results show linear correlation analyses for Ct values compared to RIN values for the genes β2 microglobulin (β2 M) ( a and b

    Journal: Cell and tissue banking

    Article Title: Characterization of RNA isolated from eighteen different human tissues: results from a rapid human autopsy program

    doi: 10.1007/s10561-016-9555-8

    Figure Lengend Snippet: Effect of RIN on expression values of housekeeping normalization genes in RNA samples derived from heart. The results show linear correlation analyses for Ct values compared to RIN values for the genes β2 microglobulin (β2 M) ( a and b

    Article Snippet: The Agilent Bioanalyzer RNA integrity number (RIN) has become the accepted standard for assessing the intactness of isolated RNA from all sources ( ).

    Techniques: Expressing, Derivative Assay

    Bioanalyzer results for total RNA isolated from (A) AS-FFPE HCC tissues, and (B) good, intermediate, and poor quality FC-FFPE tissues. The AS- and FC-FFPE RNA samples were extracted from different samples. AS: archived section, FFPE: formalin-fixed paraffin-embedded, HCC: hepatocellular carcinoma, FC: freshly cut, RIN: RNA Integrity Number.

    Journal: PLoS ONE

    Article Title: Transcriptome Profiling of Archived Sectioned Formalin-Fixed Paraffin-Embedded (AS-FFPE) Tissue for Disease Classification

    doi: 10.1371/journal.pone.0086961

    Figure Lengend Snippet: Bioanalyzer results for total RNA isolated from (A) AS-FFPE HCC tissues, and (B) good, intermediate, and poor quality FC-FFPE tissues. The AS- and FC-FFPE RNA samples were extracted from different samples. AS: archived section, FFPE: formalin-fixed paraffin-embedded, HCC: hepatocellular carcinoma, FC: freshly cut, RIN: RNA Integrity Number.

    Article Snippet: The quality of RNA was evaluated by Bioanalyzer (Agilent), and the traces and RNA Integrity Number (RIN) were compared to those of FC-FFPE RNA samples known to yield good, intermediate, or poor quality whole-transcriptome profiles (WGDASL HT Assay Guide 15018210D, Illumina) .

    Techniques: Isolation, Formalin-fixed Paraffin-Embedded