rna integrity Search Results


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  • 99
    Thermo Fisher ribonucleic acid rna integrity
    Distributionof vasa , dnd , and ly75 transcripts in Atlantic salmon. A : cDNA from various tissues of adult fish (blood, brain, gill, skeletal muscle, heart, liver, spleen, gall bladder, stomach, pyloric caeca, mid gut, head kidney, kidney, skin, testis, and ovary) were used for semi-quantitative RT-PCR. Actb was used as endogenous reference. Amplicon sizes, in base pairs, are indicated on the right. Expression pattern was determined using two biological replicates. B : Total <t>RNA</t> (400–900 ng) from early embryonic stages (two-cell, eight-cell, early-blastula, late-blastula, mid-gastrula, and 10-somite) was electrophoresed. Both 28S and 18S rRNA, stained with SYBR Safe DNA gel stain, are shown in all stages. C : The changes of both total RNA (white squares) and <t>mRNA</t> (black bars) amount per egg for each developmental stage. The concentration was quantified using three replicates. D : cDNA synthesized from above-mentioned developmental stages were used for semi-quantitative RT-PCR. In order to eliminate a possibility of genomic DNA contamination, −RT (without reverse transcriptase) samples of each counterpart were examined and electrophoresed. Amplicon sizes, in base pairs are indicated on the right.
    Ribonucleic Acid Rna Integrity, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore rna integrity
    Distributionof vasa , dnd , and ly75 transcripts in Atlantic salmon. A : cDNA from various tissues of adult fish (blood, brain, gill, skeletal muscle, heart, liver, spleen, gall bladder, stomach, pyloric caeca, mid gut, head kidney, kidney, skin, testis, and ovary) were used for semi-quantitative RT-PCR. Actb was used as endogenous reference. Amplicon sizes, in base pairs, are indicated on the right. Expression pattern was determined using two biological replicates. B : Total <t>RNA</t> (400–900 ng) from early embryonic stages (two-cell, eight-cell, early-blastula, late-blastula, mid-gastrula, and 10-somite) was electrophoresed. Both 28S and 18S rRNA, stained with SYBR Safe DNA gel stain, are shown in all stages. C : The changes of both total RNA (white squares) and <t>mRNA</t> (black bars) amount per egg for each developmental stage. The concentration was quantified using three replicates. D : cDNA synthesized from above-mentioned developmental stages were used for semi-quantitative RT-PCR. In order to eliminate a possibility of genomic DNA contamination, −RT (without reverse transcriptase) samples of each counterpart were examined and electrophoresed. Amplicon sizes, in base pairs are indicated on the right.
    Rna Integrity, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 192 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Agilent technologies ribonucleic acid rna integrity
    Relationship between SDV and <t>RIN</t> . Comparison of the SDV and RIN integrity metrics for a panel of seven <t>RNA</t> samples.
    Ribonucleic Acid Rna Integrity, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Agilent technologies integrity controlled rna
    Relationship between SDV and <t>RIN</t> . Comparison of the SDV and RIN integrity metrics for a panel of seven <t>RNA</t> samples.
    Integrity Controlled Rna, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Bio-Rad rna integrity rna integrity
    Relationship between SDV and <t>RIN</t> . Comparison of the SDV and RIN integrity metrics for a panel of seven <t>RNA</t> samples.
    Rna Integrity Rna Integrity, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Illumina Inc project integrated rna seq
    Relationship between SDV and <t>RIN</t> . Comparison of the SDV and RIN integrity metrics for a panel of seven <t>RNA</t> samples.
    Project Integrated Rna Seq, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    promega rna integrity
    Relationship between SDV and <t>RIN</t> . Comparison of the SDV and RIN integrity metrics for a panel of seven <t>RNA</t> samples.
    Rna Integrity, supplied by promega, used in various techniques. Bioz Stars score: 92/100, based on 388 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioTek Instruments rna integrity
    Relationship between SDV and <t>RIN</t> . Comparison of the SDV and RIN integrity metrics for a panel of seven <t>RNA</t> samples.
    Rna Integrity, supplied by BioTek Instruments, used in various techniques. Bioz Stars score: 92/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Rockland Immunochemicals rna integrity
    Relationship between SDV and <t>RIN</t> . Comparison of the SDV and RIN integrity metrics for a panel of seven <t>RNA</t> samples.
    Rna Integrity, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    UNICO rna integrity
    Relationship between SDV and <t>RIN</t> . Comparison of the SDV and RIN integrity metrics for a panel of seven <t>RNA</t> samples.
    Rna Integrity, supplied by UNICO, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Advanced Genomic Technology LLC rna integrity
    Relationship between SDV and <t>RIN</t> . Comparison of the SDV and RIN integrity metrics for a panel of seven <t>RNA</t> samples.
    Rna Integrity, supplied by Advanced Genomic Technology LLC, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Distributionof vasa , dnd , and ly75 transcripts in Atlantic salmon. A : cDNA from various tissues of adult fish (blood, brain, gill, skeletal muscle, heart, liver, spleen, gall bladder, stomach, pyloric caeca, mid gut, head kidney, kidney, skin, testis, and ovary) were used for semi-quantitative RT-PCR. Actb was used as endogenous reference. Amplicon sizes, in base pairs, are indicated on the right. Expression pattern was determined using two biological replicates. B : Total RNA (400–900 ng) from early embryonic stages (two-cell, eight-cell, early-blastula, late-blastula, mid-gastrula, and 10-somite) was electrophoresed. Both 28S and 18S rRNA, stained with SYBR Safe DNA gel stain, are shown in all stages. C : The changes of both total RNA (white squares) and mRNA (black bars) amount per egg for each developmental stage. The concentration was quantified using three replicates. D : cDNA synthesized from above-mentioned developmental stages were used for semi-quantitative RT-PCR. In order to eliminate a possibility of genomic DNA contamination, −RT (without reverse transcriptase) samples of each counterpart were examined and electrophoresed. Amplicon sizes, in base pairs are indicated on the right.

    Journal: Molecular Reproduction and Development

    Article Title: Identification and Migration of Primordial Germ Cells in Atlantic Salmon, Salmo salar: Characterization of Vasa, Dead End, and Lymphocyte Antigen 75 Genes

    doi: 10.1002/mrd.22142

    Figure Lengend Snippet: Distributionof vasa , dnd , and ly75 transcripts in Atlantic salmon. A : cDNA from various tissues of adult fish (blood, brain, gill, skeletal muscle, heart, liver, spleen, gall bladder, stomach, pyloric caeca, mid gut, head kidney, kidney, skin, testis, and ovary) were used for semi-quantitative RT-PCR. Actb was used as endogenous reference. Amplicon sizes, in base pairs, are indicated on the right. Expression pattern was determined using two biological replicates. B : Total RNA (400–900 ng) from early embryonic stages (two-cell, eight-cell, early-blastula, late-blastula, mid-gastrula, and 10-somite) was electrophoresed. Both 28S and 18S rRNA, stained with SYBR Safe DNA gel stain, are shown in all stages. C : The changes of both total RNA (white squares) and mRNA (black bars) amount per egg for each developmental stage. The concentration was quantified using three replicates. D : cDNA synthesized from above-mentioned developmental stages were used for semi-quantitative RT-PCR. In order to eliminate a possibility of genomic DNA contamination, −RT (without reverse transcriptase) samples of each counterpart were examined and electrophoresed. Amplicon sizes, in base pairs are indicated on the right.

    Article Snippet: Total RNA and purified mRNA were electrophoresed on a 1% (w/v) agarose gel to assessed RNA integrity, and were further quantified with a NanoDrop ND-1000 (Thermo Scientific, Saven & Werner AS, Kristiansand, Norway).

    Techniques: Fluorescence In Situ Hybridization, Quantitative RT-PCR, Amplification, Expressing, Staining, Concentration Assay, Synthesized

    Relationship between SDV and RIN . Comparison of the SDV and RIN integrity metrics for a panel of seven RNA samples.

    Journal: BMC Research Notes

    Article Title: Evaluation of a novel approach for the measurement of RNA quality

    doi: 10.1186/1756-0500-3-89

    Figure Lengend Snippet: Relationship between SDV and RIN . Comparison of the SDV and RIN integrity metrics for a panel of seven RNA samples. "Treatment" denotes the duration of thermal degradation treatment used. Each group includes nine observations, except for treatment four which corresponds to a 12-minute incubation at 90°C and, which includes only seven observations. The thicker horizontal line shows the median, boxes show the upper and lower quartiles and whiskers extend to the most distant data point within 1.5 times the interquartile range of the relevant quartile. Values beyond this are shown as individual data points.

    Article Snippet: Comparison with RNA Integrity Number (RIN) RIN is an incremental scale which spans from 0 to 10, with increasing RNA integrity correlating with increasing RIN value.

    Techniques: Incubation

    SDV and RIN chromatograms . Graphical overlay of SDV chromatograms for the analysis of intact (A), and degraded (B) RNA. RIN chromatograms for intact (C) and degraded (D) RNA are shown for comparison.

    Journal: BMC Research Notes

    Article Title: Evaluation of a novel approach for the measurement of RNA quality

    doi: 10.1186/1756-0500-3-89

    Figure Lengend Snippet: SDV and RIN chromatograms . Graphical overlay of SDV chromatograms for the analysis of intact (A), and degraded (B) RNA. RIN chromatograms for intact (C) and degraded (D) RNA are shown for comparison.

    Article Snippet: Comparison with RNA Integrity Number (RIN) RIN is an incremental scale which spans from 0 to 10, with increasing RNA integrity correlating with increasing RIN value.

    Techniques:

    Variation of RNA integrity in primary tumors and metastases in a metastatic cancer case A129 ( A ) One primary pancreatic tumor was bread-loafed as indicated by primary tumor section S2 to S6. Each slice was further cut into equally sized small pieces (approximately 1 × 1 cm) and numbered accordingly to generate sample IDs. Samples with high tumor cellularity were used for RNA extraction and determination of RNA integrity. Samples indicated by an asterisk (*) were excluded due to low tumor cellularity or necrosis. ( B ) Each piece of tissue was trisected and RNA extracted (E1,E2,E3). The color code for RIN value is the same as in Figure 1 .

    Journal: Oncotarget

    Article Title: Quantification of nucleic acid quality in postmortem tissues from a cancer research autopsy program

    doi: 10.18632/oncotarget.11836

    Figure Lengend Snippet: Variation of RNA integrity in primary tumors and metastases in a metastatic cancer case A129 ( A ) One primary pancreatic tumor was bread-loafed as indicated by primary tumor section S2 to S6. Each slice was further cut into equally sized small pieces (approximately 1 × 1 cm) and numbered accordingly to generate sample IDs. Samples with high tumor cellularity were used for RNA extraction and determination of RNA integrity. Samples indicated by an asterisk (*) were excluded due to low tumor cellularity or necrosis. ( B ) Each piece of tissue was trisected and RNA extracted (E1,E2,E3). The color code for RIN value is the same as in Figure 1 .

    Article Snippet: RNA integrity analysis The RNA integrity number (RIN) was determined using an Agilent 2100 Bioanalyzer (Agilent Technologies) with an RNA nano-kit system as described in the manufacturer's instructions.

    Techniques: RNA Extraction

    RNAseq clearly separates a tumor signature from the normal counterpart ( A ) Normalized RNA-seq coverage is plotted against transcript position. ( B ) Fragment length distribution. Representative samples with wide spectrum of RIN are shown in A and B. ( C ) Heat map shows expression profile of top 250 differentially expressed genes. Numbers in parenthesis indicate RIN values. Tumor, tissues from primary pancreatic cancer. Normal, tissue from normal pancreas.

    Journal: Oncotarget

    Article Title: Quantification of nucleic acid quality in postmortem tissues from a cancer research autopsy program

    doi: 10.18632/oncotarget.11836

    Figure Lengend Snippet: RNAseq clearly separates a tumor signature from the normal counterpart ( A ) Normalized RNA-seq coverage is plotted against transcript position. ( B ) Fragment length distribution. Representative samples with wide spectrum of RIN are shown in A and B. ( C ) Heat map shows expression profile of top 250 differentially expressed genes. Numbers in parenthesis indicate RIN values. Tumor, tissues from primary pancreatic cancer. Normal, tissue from normal pancreas.

    Article Snippet: RNA integrity analysis The RNA integrity number (RIN) was determined using an Agilent 2100 Bioanalyzer (Agilent Technologies) with an RNA nano-kit system as described in the manufacturer's instructions.

    Techniques: RNA Sequencing Assay, Expressing

    RNA integrity numbers in patient-matched tissues RIN values from patient-matched tissues were plotted against tissue types. Selected cases from each of the four PMI categories were shown. Samples with low RNA yield leading to unreported or unreliable RIN were assigned to a RIN value of 0 and indicated by a circle. Dashed lines indicate tissues not analysed for that patient. Abbreviations are He, heart; Ki, kidney; Li, liver; Lu, Lung; Pa, pancreas; Ske, skeletal muscle; Ski, skin; Sp, spleen; Pr, primary tumor; LiM, liver mets; LuM, Lung mets.

    Journal: Oncotarget

    Article Title: Quantification of nucleic acid quality in postmortem tissues from a cancer research autopsy program

    doi: 10.18632/oncotarget.11836

    Figure Lengend Snippet: RNA integrity numbers in patient-matched tissues RIN values from patient-matched tissues were plotted against tissue types. Selected cases from each of the four PMI categories were shown. Samples with low RNA yield leading to unreported or unreliable RIN were assigned to a RIN value of 0 and indicated by a circle. Dashed lines indicate tissues not analysed for that patient. Abbreviations are He, heart; Ki, kidney; Li, liver; Lu, Lung; Pa, pancreas; Ske, skeletal muscle; Ski, skin; Sp, spleen; Pr, primary tumor; LiM, liver mets; LuM, Lung mets.

    Article Snippet: RNA integrity analysis The RNA integrity number (RIN) was determined using an Agilent 2100 Bioanalyzer (Agilent Technologies) with an RNA nano-kit system as described in the manufacturer's instructions.

    Techniques:

    Correlations between RNA integrity numbers and post mortem interval by tissue site Scatter plots were generated by plotting RIN values from each normal tissue type or primary tumors against PMI. Linear regression was performed to create curve fits. Samples with low RNA yield leading to unreported or unreliable RIN were assigned to a RIN value of 0 (red stars) and excluded from linear regression analysis.

    Journal: Oncotarget

    Article Title: Quantification of nucleic acid quality in postmortem tissues from a cancer research autopsy program

    doi: 10.18632/oncotarget.11836

    Figure Lengend Snippet: Correlations between RNA integrity numbers and post mortem interval by tissue site Scatter plots were generated by plotting RIN values from each normal tissue type or primary tumors against PMI. Linear regression was performed to create curve fits. Samples with low RNA yield leading to unreported or unreliable RIN were assigned to a RIN value of 0 (red stars) and excluded from linear regression analysis.

    Article Snippet: RNA integrity analysis The RNA integrity number (RIN) was determined using an Agilent 2100 Bioanalyzer (Agilent Technologies) with an RNA nano-kit system as described in the manufacturer's instructions.

    Techniques: Generated

    Correlation between RIN and sample storage time Scatter plots were generated by plotting RIN values from each tissue types against sample storage time. Samples with low RNA yield leading to unreported or unreliable RIN were assigned to a RIN value of 0 and indicated by a red star.

    Journal: Oncotarget

    Article Title: Quantification of nucleic acid quality in postmortem tissues from a cancer research autopsy program

    doi: 10.18632/oncotarget.11836

    Figure Lengend Snippet: Correlation between RIN and sample storage time Scatter plots were generated by plotting RIN values from each tissue types against sample storage time. Samples with low RNA yield leading to unreported or unreliable RIN were assigned to a RIN value of 0 and indicated by a red star.

    Article Snippet: RNA integrity analysis The RNA integrity number (RIN) was determined using an Agilent 2100 Bioanalyzer (Agilent Technologies) with an RNA nano-kit system as described in the manufacturer's instructions.

    Techniques: Generated

    RNA integrity in patient-matched liver metastases and lung metastases ( A ) Numbers listed are the corresponding RIN value for each individual tissue. N/A, data not available due to low RNA yield. ( B ) Scatter plot was generated by plotting RIN values from liver metastases against that from lung metastases. Pearson r and p value s were from correlation analysis.

    Journal: Oncotarget

    Article Title: Quantification of nucleic acid quality in postmortem tissues from a cancer research autopsy program

    doi: 10.18632/oncotarget.11836

    Figure Lengend Snippet: RNA integrity in patient-matched liver metastases and lung metastases ( A ) Numbers listed are the corresponding RIN value for each individual tissue. N/A, data not available due to low RNA yield. ( B ) Scatter plot was generated by plotting RIN values from liver metastases against that from lung metastases. Pearson r and p value s were from correlation analysis.

    Article Snippet: RNA integrity analysis The RNA integrity number (RIN) was determined using an Agilent 2100 Bioanalyzer (Agilent Technologies) with an RNA nano-kit system as described in the manufacturer's instructions.

    Techniques: Generated

    Heat map of RNA integrity numbers by tissue type RIN values from individual tissues were expressed with colored regions as defined by the accompanying legend. Abbreviations are PDA, pancreatic ductal adenocarcinoma; BC, breast cancer; GCT, germ cell tumor; CRC, colorectal cancer; NSCLC, non-small cell lung cancer; UCC, urothelial cell carcinoma; MM, melanoma; He, heart; Ki, kidney; Li, liver; Lu, Lung; Pa, pancreas; Ski, skin; Sp, spleen; Pr, primary tumor; LiM, liver metastasis; LuM, Lung metastasis. PMA, postmortem autolysis; TN, tumor-associated necrosis; fib, fibrosis.

    Journal: Oncotarget

    Article Title: Quantification of nucleic acid quality in postmortem tissues from a cancer research autopsy program

    doi: 10.18632/oncotarget.11836

    Figure Lengend Snippet: Heat map of RNA integrity numbers by tissue type RIN values from individual tissues were expressed with colored regions as defined by the accompanying legend. Abbreviations are PDA, pancreatic ductal adenocarcinoma; BC, breast cancer; GCT, germ cell tumor; CRC, colorectal cancer; NSCLC, non-small cell lung cancer; UCC, urothelial cell carcinoma; MM, melanoma; He, heart; Ki, kidney; Li, liver; Lu, Lung; Pa, pancreas; Ski, skin; Sp, spleen; Pr, primary tumor; LiM, liver metastasis; LuM, Lung metastasis. PMA, postmortem autolysis; TN, tumor-associated necrosis; fib, fibrosis.

    Article Snippet: RNA integrity analysis The RNA integrity number (RIN) was determined using an Agilent 2100 Bioanalyzer (Agilent Technologies) with an RNA nano-kit system as described in the manufacturer's instructions.

    Techniques:

    RNA integrity and concentration. A) Gel-eletrophoretic separation of isolated total RNA after DNase digest and clean-up on 1% agarose. B) Gel images of RNA samples generated by the Agilent Bioanalyzer using RNA 6000 Nano Lab Chip. C) RNA concentration and RIN as determined by the Agilent Bioanalyzer. RIN = RNA Integrity Number; L = RNA Ladder. Green line in Figure 4B: Bioanalyzer internal marker.

    Journal: PLoS ONE

    Article Title: Probing Oral Microbial Functionality - Expression of spxB in Plaque Samples

    doi: 10.1371/journal.pone.0086685

    Figure Lengend Snippet: RNA integrity and concentration. A) Gel-eletrophoretic separation of isolated total RNA after DNase digest and clean-up on 1% agarose. B) Gel images of RNA samples generated by the Agilent Bioanalyzer using RNA 6000 Nano Lab Chip. C) RNA concentration and RIN as determined by the Agilent Bioanalyzer. RIN = RNA Integrity Number; L = RNA Ladder. Green line in Figure 4B: Bioanalyzer internal marker.

    Article Snippet: RNA Integrity RNA integrity was determined using an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc.).

    Techniques: Concentration Assay, Isolation, Generated, Chromatin Immunoprecipitation, Marker