rna fragmentation reagents Thermo Fisher Search Results


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  • 99
    Thermo Fisher rna fragmentation reagents
    Rna Fragmentation Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1002 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rna fragmentation reagent
    Rna Fragmentation Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 333 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rna storage buffer
    Rna Storage Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 208 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rna
    Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 177224 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher genechip dna labelling reagents
    Genechip Dna Labelling Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pri mirna
    Pri Mirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 221 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher accuprime taq dna polymerase reagents
    Accuprime Taq Dna Polymerase Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rna solution
    Rna Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 876 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher platinum taqdna polymerase high fidelity reagents
    Platinum Taqdna Polymerase High Fidelity Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rna stabilization solution
    Rna Stabilization Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 393 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher trizol reagent
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    Thermo Fisher tri reagent
    Tri Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 8550 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher trizol ls reagent
    Trizol Ls Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8918 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quant it picogreen dsdna reagent
    Quant It Picogreen Dsdna Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1874 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher cleansweep pcr purification reagent
    Cleansweep Pcr Purification Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher brdu
    MiR-630 inhibits cell proliferation by inhibiting CDC7-mediated DNA synthesis. A549 cells were transfected with scrambled small interfering RNA (siRNA) (control), miR-630 mimic and miR-630 inhibitor for 48 and 72 h. ( a ) Western blotting for CDC7, MCM2 and phospho-MCM2. β -Actin was used as a loading control. ( b ) Flow cytometric analysis of <t>BrdU-positive</t> cells. After 48 and 72 h transfection, cells were labeled with 50 μ M BrdU for 1 h before collection. Samples were stained with anti-BrdU <t>FITC</t> antibody and PI, and analyzed by flow cytometry. BrdU-positive cells were included in the gate region. ( c ) Relative amount of BrdU-positive cells in the gate region compared with total cells in ( b ) experiments. Data are presented as mean±S.D. ( n =3); 48 h: * P =0.0396, ## P =0.0054; 72 h: ** P =0.0037, # P =0.0171. ( d ) MTS assay showed the survival of A549 cells transfected with miR-630 mimic or inhibitor for 48 h. Data are presented as mean±S.D. ( n =3); ** P =0.0026 and # P =0.0223
    Brdu, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 4373 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ion total rna seq kit
    MiR-630 inhibits cell proliferation by inhibiting CDC7-mediated DNA synthesis. A549 cells were transfected with scrambled small interfering RNA (siRNA) (control), miR-630 mimic and miR-630 inhibitor for 48 and 72 h. ( a ) Western blotting for CDC7, MCM2 and phospho-MCM2. β -Actin was used as a loading control. ( b ) Flow cytometric analysis of <t>BrdU-positive</t> cells. After 48 and 72 h transfection, cells were labeled with 50 μ M BrdU for 1 h before collection. Samples were stained with anti-BrdU <t>FITC</t> antibody and PI, and analyzed by flow cytometry. BrdU-positive cells were included in the gate region. ( c ) Relative amount of BrdU-positive cells in the gate region compared with total cells in ( b ) experiments. Data are presented as mean±S.D. ( n =3); 48 h: * P =0.0396, ## P =0.0054; 72 h: ** P =0.0037, # P =0.0171. ( d ) MTS assay showed the survival of A549 cells transfected with miR-630 mimic or inhibitor for 48 h. Data are presented as mean±S.D. ( n =3); ** P =0.0026 and # P =0.0223
    Ion Total Rna Seq Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 380 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher genechip 3 ivt
    MiR-630 inhibits cell proliferation by inhibiting CDC7-mediated DNA synthesis. A549 cells were transfected with scrambled small interfering RNA (siRNA) (control), miR-630 mimic and miR-630 inhibitor for 48 and 72 h. ( a ) Western blotting for CDC7, MCM2 and phospho-MCM2. β -Actin was used as a loading control. ( b ) Flow cytometric analysis of <t>BrdU-positive</t> cells. After 48 and 72 h transfection, cells were labeled with 50 μ M BrdU for 1 h before collection. Samples were stained with anti-BrdU <t>FITC</t> antibody and PI, and analyzed by flow cytometry. BrdU-positive cells were included in the gate region. ( c ) Relative amount of BrdU-positive cells in the gate region compared with total cells in ( b ) experiments. Data are presented as mean±S.D. ( n =3); 48 h: * P =0.0396, ## P =0.0054; 72 h: ** P =0.0037, # P =0.0171. ( d ) MTS assay showed the survival of A549 cells transfected with miR-630 mimic or inhibitor for 48 h. Data are presented as mean±S.D. ( n =3); ** P =0.0026 and # P =0.0223
    Genechip 3 Ivt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher cytoscan hd array kit
    MiR-630 inhibits cell proliferation by inhibiting CDC7-mediated DNA synthesis. A549 cells were transfected with scrambled small interfering RNA (siRNA) (control), miR-630 mimic and miR-630 inhibitor for 48 and 72 h. ( a ) Western blotting for CDC7, MCM2 and phospho-MCM2. β -Actin was used as a loading control. ( b ) Flow cytometric analysis of <t>BrdU-positive</t> cells. After 48 and 72 h transfection, cells were labeled with 50 μ M BrdU for 1 h before collection. Samples were stained with anti-BrdU <t>FITC</t> antibody and PI, and analyzed by flow cytometry. BrdU-positive cells were included in the gate region. ( c ) Relative amount of BrdU-positive cells in the gate region compared with total cells in ( b ) experiments. Data are presented as mean±S.D. ( n =3); 48 h: * P =0.0396, ## P =0.0054; 72 h: ** P =0.0037, # P =0.0171. ( d ) MTS assay showed the survival of A549 cells transfected with miR-630 mimic or inhibitor for 48 h. Data are presented as mean±S.D. ( n =3); ** P =0.0026 and # P =0.0223
    Cytoscan Hd Array Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher genechip resequencing reagent kit
    Affymetrix File Types. Adapted from Affymetrix <t>GeneChip</t> Sequence Analysis Software User Guide.
    Genechip Resequencing Reagent Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher wt pico reagent kit protocol
    Affymetrix File Types. Adapted from Affymetrix <t>GeneChip</t> Sequence Analysis Software User Guide.
    Wt Pico Reagent Kit Protocol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher nuclear staining reagent hoechst 33342
    Affymetrix File Types. Adapted from Affymetrix <t>GeneChip</t> Sequence Analysis Software User Guide.
    Nuclear Staining Reagent Hoechst 33342, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher reagent apo brdu tunel assay kit
    Cell death/proliferation in the androgen-resistant DU145 prostate cancer cells in response to wt/nMMM-ANXA7 and p53. ( a ) Average results from replicate experiments on cytotoxicity (LDH) and viability (XTT) represented levels relative to vector control (100%). Cell cycling ( b ) and <t>APO-BRDU-derived</t> PCD ( c ) data represented the differences (delta-%) compared to adenoviral vector control <t>(TUNEL-based</t> apoptotic rates and cell numbers in different cell cycle phases). Statistically significant p-values were designated by an asterisk (*). ( d ) Images and numbers were representative of ANXA V-PE- derived PCD with phosphatidylserine exposure and membrane permeabilization (bottom and top right quadrants, respectively) from replicates.
    Reagent Apo Brdu Tunel Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher stop solution
    Cell death/proliferation in the androgen-resistant DU145 prostate cancer cells in response to wt/nMMM-ANXA7 and p53. ( a ) Average results from replicate experiments on cytotoxicity (LDH) and viability (XTT) represented levels relative to vector control (100%). Cell cycling ( b ) and <t>APO-BRDU-derived</t> PCD ( c ) data represented the differences (delta-%) compared to adenoviral vector control <t>(TUNEL-based</t> apoptotic rates and cell numbers in different cell cycle phases). Statistically significant p-values were designated by an asterisk (*). ( d ) Images and numbers were representative of ANXA V-PE- derived PCD with phosphatidylserine exposure and membrane permeabilization (bottom and top right quadrants, respectively) from replicates.
    Stop Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1053 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher tris
    Cell death/proliferation in the androgen-resistant DU145 prostate cancer cells in response to wt/nMMM-ANXA7 and p53. ( a ) Average results from replicate experiments on cytotoxicity (LDH) and viability (XTT) represented levels relative to vector control (100%). Cell cycling ( b ) and <t>APO-BRDU-derived</t> PCD ( c ) data represented the differences (delta-%) compared to adenoviral vector control <t>(TUNEL-based</t> apoptotic rates and cell numbers in different cell cycle phases). Statistically significant p-values were designated by an asterisk (*). ( d ) Images and numbers were representative of ANXA V-PE- derived PCD with phosphatidylserine exposure and membrane permeabilization (bottom and top right quadrants, respectively) from replicates.
    Tris, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 11208 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ion plus fragment library kit
    Cell death/proliferation in the androgen-resistant DU145 prostate cancer cells in response to wt/nMMM-ANXA7 and p53. ( a ) Average results from replicate experiments on cytotoxicity (LDH) and viability (XTT) represented levels relative to vector control (100%). Cell cycling ( b ) and <t>APO-BRDU-derived</t> PCD ( c ) data represented the differences (delta-%) compared to adenoviral vector control <t>(TUNEL-based</t> apoptotic rates and cell numbers in different cell cycle phases). Statistically significant p-values were designated by an asterisk (*). ( d ) Images and numbers were representative of ANXA V-PE- derived PCD with phosphatidylserine exposure and membrane permeabilization (bottom and top right quadrants, respectively) from replicates.
    Ion Plus Fragment Library Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1484 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ammonium acetate
    Cell death/proliferation in the androgen-resistant DU145 prostate cancer cells in response to wt/nMMM-ANXA7 and p53. ( a ) Average results from replicate experiments on cytotoxicity (LDH) and viability (XTT) represented levels relative to vector control (100%). Cell cycling ( b ) and <t>APO-BRDU-derived</t> PCD ( c ) data represented the differences (delta-%) compared to adenoviral vector control <t>(TUNEL-based</t> apoptotic rates and cell numbers in different cell cycle phases). Statistically significant p-values were designated by an asterisk (*). ( d ) Images and numbers were representative of ANXA V-PE- derived PCD with phosphatidylserine exposure and membrane permeabilization (bottom and top right quadrants, respectively) from replicates.
    Ammonium Acetate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1295 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sds electrophoresis
    Cell death/proliferation in the androgen-resistant DU145 prostate cancer cells in response to wt/nMMM-ANXA7 and p53. ( a ) Average results from replicate experiments on cytotoxicity (LDH) and viability (XTT) represented levels relative to vector control (100%). Cell cycling ( b ) and <t>APO-BRDU-derived</t> PCD ( c ) data represented the differences (delta-%) compared to adenoviral vector control <t>(TUNEL-based</t> apoptotic rates and cell numbers in different cell cycle phases). Statistically significant p-values were designated by an asterisk (*). ( d ) Images and numbers were representative of ANXA V-PE- derived PCD with phosphatidylserine exposure and membrane permeabilization (bottom and top right quadrants, respectively) from replicates.
    Sds Electrophoresis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sodium acetate
    Cell death/proliferation in the androgen-resistant DU145 prostate cancer cells in response to wt/nMMM-ANXA7 and p53. ( a ) Average results from replicate experiments on cytotoxicity (LDH) and viability (XTT) represented levels relative to vector control (100%). Cell cycling ( b ) and <t>APO-BRDU-derived</t> PCD ( c ) data represented the differences (delta-%) compared to adenoviral vector control <t>(TUNEL-based</t> apoptotic rates and cell numbers in different cell cycle phases). Statistically significant p-values were designated by an asterisk (*). ( d ) Images and numbers were representative of ANXA V-PE- derived PCD with phosphatidylserine exposure and membrane permeabilization (bottom and top right quadrants, respectively) from replicates.
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    Thermo Fisher phosphoramidite dye 6 fam
    Cell death/proliferation in the androgen-resistant DU145 prostate cancer cells in response to wt/nMMM-ANXA7 and p53. ( a ) Average results from replicate experiments on cytotoxicity (LDH) and viability (XTT) represented levels relative to vector control (100%). Cell cycling ( b ) and <t>APO-BRDU-derived</t> PCD ( c ) data represented the differences (delta-%) compared to adenoviral vector control <t>(TUNEL-based</t> apoptotic rates and cell numbers in different cell cycle phases). Statistically significant p-values were designated by an asterisk (*). ( d ) Images and numbers were representative of ANXA V-PE- derived PCD with phosphatidylserine exposure and membrane permeabilization (bottom and top right quadrants, respectively) from replicates.
    Phosphoramidite Dye 6 Fam, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    MiR-630 inhibits cell proliferation by inhibiting CDC7-mediated DNA synthesis. A549 cells were transfected with scrambled small interfering RNA (siRNA) (control), miR-630 mimic and miR-630 inhibitor for 48 and 72 h. ( a ) Western blotting for CDC7, MCM2 and phospho-MCM2. β -Actin was used as a loading control. ( b ) Flow cytometric analysis of BrdU-positive cells. After 48 and 72 h transfection, cells were labeled with 50 μ M BrdU for 1 h before collection. Samples were stained with anti-BrdU FITC antibody and PI, and analyzed by flow cytometry. BrdU-positive cells were included in the gate region. ( c ) Relative amount of BrdU-positive cells in the gate region compared with total cells in ( b ) experiments. Data are presented as mean±S.D. ( n =3); 48 h: * P =0.0396, ## P =0.0054; 72 h: ** P =0.0037, # P =0.0171. ( d ) MTS assay showed the survival of A549 cells transfected with miR-630 mimic or inhibitor for 48 h. Data are presented as mean±S.D. ( n =3); ** P =0.0026 and # P =0.0223

    Journal: Cell Death & Disease

    Article Title: MiR-630 inhibits proliferation by targeting CDC7 kinase, but maintains the apoptotic balance by targeting multiple modulators in human lung cancer A549 cells

    doi: 10.1038/cddis.2014.386

    Figure Lengend Snippet: MiR-630 inhibits cell proliferation by inhibiting CDC7-mediated DNA synthesis. A549 cells were transfected with scrambled small interfering RNA (siRNA) (control), miR-630 mimic and miR-630 inhibitor for 48 and 72 h. ( a ) Western blotting for CDC7, MCM2 and phospho-MCM2. β -Actin was used as a loading control. ( b ) Flow cytometric analysis of BrdU-positive cells. After 48 and 72 h transfection, cells were labeled with 50 μ M BrdU for 1 h before collection. Samples were stained with anti-BrdU FITC antibody and PI, and analyzed by flow cytometry. BrdU-positive cells were included in the gate region. ( c ) Relative amount of BrdU-positive cells in the gate region compared with total cells in ( b ) experiments. Data are presented as mean±S.D. ( n =3); 48 h: * P =0.0396, ## P =0.0054; 72 h: ** P =0.0037, # P =0.0171. ( d ) MTS assay showed the survival of A549 cells transfected with miR-630 mimic or inhibitor for 48 h. Data are presented as mean±S.D. ( n =3); ** P =0.0026 and # P =0.0223

    Article Snippet: Incorporated BrdU was detected using FITC-conjugated anti-BrdU antibody (11-5071; eBioscience, San Diego, CA, USA).

    Techniques: DNA Synthesis, Transfection, Small Interfering RNA, Western Blot, Flow Cytometry, Labeling, Staining, Cytometry, MTS Assay

    Affymetrix File Types. Adapted from Affymetrix GeneChip Sequence Analysis Software User Guide.

    Journal: Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]

    Article Title: An Overview of Custom Array Sequencing

    doi: 10.1002/0471142905.hg0717s61

    Figure Lengend Snippet: Affymetrix File Types. Adapted from Affymetrix GeneChip Sequence Analysis Software User Guide.

    Article Snippet: A fragmentation enzyme is part of the Affymetrix GeneChip Resequencing Reagent Kit.

    Techniques: Sequencing, Software

    Cell death/proliferation in the androgen-resistant DU145 prostate cancer cells in response to wt/nMMM-ANXA7 and p53. ( a ) Average results from replicate experiments on cytotoxicity (LDH) and viability (XTT) represented levels relative to vector control (100%). Cell cycling ( b ) and APO-BRDU-derived PCD ( c ) data represented the differences (delta-%) compared to adenoviral vector control (TUNEL-based apoptotic rates and cell numbers in different cell cycle phases). Statistically significant p-values were designated by an asterisk (*). ( d ) Images and numbers were representative of ANXA V-PE- derived PCD with phosphatidylserine exposure and membrane permeabilization (bottom and top right quadrants, respectively) from replicates.

    Journal: Trends in cancer research

    Article Title: Cyclin E and FGF8 are downstream cell growth regulators in distinct tumor suppressor effects of ANXA7 in hormone-resistant cancer cells of breast versus prostate origin

    doi:

    Figure Lengend Snippet: Cell death/proliferation in the androgen-resistant DU145 prostate cancer cells in response to wt/nMMM-ANXA7 and p53. ( a ) Average results from replicate experiments on cytotoxicity (LDH) and viability (XTT) represented levels relative to vector control (100%). Cell cycling ( b ) and APO-BRDU-derived PCD ( c ) data represented the differences (delta-%) compared to adenoviral vector control (TUNEL-based apoptotic rates and cell numbers in different cell cycle phases). Statistically significant p-values were designated by an asterisk (*). ( d ) Images and numbers were representative of ANXA V-PE- derived PCD with phosphatidylserine exposure and membrane permeabilization (bottom and top right quadrants, respectively) from replicates.

    Article Snippet: We bought the reagent APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU from ThermoFisher.

    Techniques: Plasmid Preparation, Derivative Assay, TUNEL Assay