rna estimation Search Results


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  • 99
    Qiagen carrier rna
    Carrier Rna, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 647 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher rna
    RT-PCR analysis of PKS gene expression in infected leaves and mycelial cultures. M . fijiensis isolate 10CR1-24 was used to infect banana tissue-culture plants, as well as to inoculate PDB medium. RT-PCR assays for each PKS or hybrid PKS-NRPS were performed on the resulting leaf lesions or mycelium from the PDB liquid culture. Where possible (for all genes except beta-tubulin and PKS10-1 ), primers were designed to span an intron, to distinguish <t>cDNA</t> from any gDNA contamination. Genomic DNA isolated from 10CR1-24 was used as a positive control, and reactions were also done with no added template, as a negative control. Each lane represents <t>RNA</t> isolated from individual infected leaves (left) or mycelium grown in flask culture (right).
    Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 177240 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore rna
    OND depletes intracellular ATP levels, inhibits transcription, induces relocation of the cellular polyamine pool to the nucleus and reduces the staining density of histone H3 with antibody. a The intracellular concentration of ATP in untreated, OND-exposed and recovering cells was determined using a luciferase dependent assay. b Global rates of transcription, determined by the incorporation of <t>bromouridine</t> into <t>RNA,</t> in untreated cells, cells under OND and cells recovering from OND are presented. HL-1 cells, either untreated or subjected to 1 hour of OND, were fixed, permeabilized and stained either with anti-polyamine antibody ( c , d ) or with anti-total H3 antibody ( e , f ) and counterstained with the fluorescent DNA binding dye Hoechst 33342. Cells were then examined using confocal microscopy. The content of immunostained histone H3 was evaluated by SMLM in untreated ( e ) and OND-treated ( f ) HL-1 cells. BrU bromouridine. The error bars represent the standard deviation of three independent samples
    Rna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 9883 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Worthington Biochemical rna
    OND depletes intracellular ATP levels, inhibits transcription, induces relocation of the cellular polyamine pool to the nucleus and reduces the staining density of histone H3 with antibody. a The intracellular concentration of ATP in untreated, OND-exposed and recovering cells was determined using a luciferase dependent assay. b Global rates of transcription, determined by the incorporation of <t>bromouridine</t> into <t>RNA,</t> in untreated cells, cells under OND and cells recovering from OND are presented. HL-1 cells, either untreated or subjected to 1 hour of OND, were fixed, permeabilized and stained either with anti-polyamine antibody ( c , d ) or with anti-total H3 antibody ( e , f ) and counterstained with the fluorescent DNA binding dye Hoechst 33342. Cells were then examined using confocal microscopy. The content of immunostained histone H3 was evaluated by SMLM in untreated ( e ) and OND-treated ( f ) HL-1 cells. BrU bromouridine. The error bars represent the standard deviation of three independent samples
    Rna, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Molecular Research Center inc total ribonucleic acid rna
    OND depletes intracellular ATP levels, inhibits transcription, induces relocation of the cellular polyamine pool to the nucleus and reduces the staining density of histone H3 with antibody. a The intracellular concentration of ATP in untreated, OND-exposed and recovering cells was determined using a luciferase dependent assay. b Global rates of transcription, determined by the incorporation of <t>bromouridine</t> into <t>RNA,</t> in untreated cells, cells under OND and cells recovering from OND are presented. HL-1 cells, either untreated or subjected to 1 hour of OND, were fixed, permeabilized and stained either with anti-polyamine antibody ( c , d ) or with anti-total H3 antibody ( e , f ) and counterstained with the fluorescent DNA binding dye Hoechst 33342. Cells were then examined using confocal microscopy. The content of immunostained histone H3 was evaluated by SMLM in untreated ( e ) and OND-treated ( f ) HL-1 cells. BrU bromouridine. The error bars represent the standard deviation of three independent samples
    Total Ribonucleic Acid Rna, supplied by Molecular Research Center inc, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    ATCC ribosomal ribonucleic acid
    OND depletes intracellular ATP levels, inhibits transcription, induces relocation of the cellular polyamine pool to the nucleus and reduces the staining density of histone H3 with antibody. a The intracellular concentration of ATP in untreated, OND-exposed and recovering cells was determined using a luciferase dependent assay. b Global rates of transcription, determined by the incorporation of <t>bromouridine</t> into <t>RNA,</t> in untreated cells, cells under OND and cells recovering from OND are presented. HL-1 cells, either untreated or subjected to 1 hour of OND, were fixed, permeabilized and stained either with anti-polyamine antibody ( c , d ) or with anti-total H3 antibody ( e , f ) and counterstained with the fluorescent DNA binding dye Hoechst 33342. Cells were then examined using confocal microscopy. The content of immunostained histone H3 was evaluated by SMLM in untreated ( e ) and OND-treated ( f ) HL-1 cells. BrU bromouridine. The error bars represent the standard deviation of three independent samples
    Ribosomal Ribonucleic Acid, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Abbott Laboratories hiv 1 ribonucleic acid rna
    OND depletes intracellular ATP levels, inhibits transcription, induces relocation of the cellular polyamine pool to the nucleus and reduces the staining density of histone H3 with antibody. a The intracellular concentration of ATP in untreated, OND-exposed and recovering cells was determined using a luciferase dependent assay. b Global rates of transcription, determined by the incorporation of <t>bromouridine</t> into <t>RNA,</t> in untreated cells, cells under OND and cells recovering from OND are presented. HL-1 cells, either untreated or subjected to 1 hour of OND, were fixed, permeabilized and stained either with anti-polyamine antibody ( c , d ) or with anti-total H3 antibody ( e , f ) and counterstained with the fluorescent DNA binding dye Hoechst 33342. Cells were then examined using confocal microscopy. The content of immunostained histone H3 was evaluated by SMLM in untreated ( e ) and OND-treated ( f ) HL-1 cells. BrU bromouridine. The error bars represent the standard deviation of three independent samples
    Hiv 1 Ribonucleic Acid Rna, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Illumina Inc ribonucleic acid rna seq transcriptome data
    OND depletes intracellular ATP levels, inhibits transcription, induces relocation of the cellular polyamine pool to the nucleus and reduces the staining density of histone H3 with antibody. a The intracellular concentration of ATP in untreated, OND-exposed and recovering cells was determined using a luciferase dependent assay. b Global rates of transcription, determined by the incorporation of <t>bromouridine</t> into <t>RNA,</t> in untreated cells, cells under OND and cells recovering from OND are presented. HL-1 cells, either untreated or subjected to 1 hour of OND, were fixed, permeabilized and stained either with anti-polyamine antibody ( c , d ) or with anti-total H3 antibody ( e , f ) and counterstained with the fluorescent DNA binding dye Hoechst 33342. Cells were then examined using confocal microscopy. The content of immunostained histone H3 was evaluated by SMLM in untreated ( e ) and OND-treated ( f ) HL-1 cells. BrU bromouridine. The error bars represent the standard deviation of three independent samples
    Ribonucleic Acid Rna Seq Transcriptome Data, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Elabscience cytokine estimation
    OND depletes intracellular ATP levels, inhibits transcription, induces relocation of the cellular polyamine pool to the nucleus and reduces the staining density of histone H3 with antibody. a The intracellular concentration of ATP in untreated, OND-exposed and recovering cells was determined using a luciferase dependent assay. b Global rates of transcription, determined by the incorporation of <t>bromouridine</t> into <t>RNA,</t> in untreated cells, cells under OND and cells recovering from OND are presented. HL-1 cells, either untreated or subjected to 1 hour of OND, were fixed, permeabilized and stained either with anti-polyamine antibody ( c , d ) or with anti-total H3 antibody ( e , f ) and counterstained with the fluorescent DNA binding dye Hoechst 33342. Cells were then examined using confocal microscopy. The content of immunostained histone H3 was evaluated by SMLM in untreated ( e ) and OND-treated ( f ) HL-1 cells. BrU bromouridine. The error bars represent the standard deviation of three independent samples
    Cytokine Estimation, supplied by Elabscience, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rna  (Lonza)
    99
    Lonza rna
    OND depletes intracellular ATP levels, inhibits transcription, induces relocation of the cellular polyamine pool to the nucleus and reduces the staining density of histone H3 with antibody. a The intracellular concentration of ATP in untreated, OND-exposed and recovering cells was determined using a luciferase dependent assay. b Global rates of transcription, determined by the incorporation of <t>bromouridine</t> into <t>RNA,</t> in untreated cells, cells under OND and cells recovering from OND are presented. HL-1 cells, either untreated or subjected to 1 hour of OND, were fixed, permeabilized and stained either with anti-polyamine antibody ( c , d ) or with anti-total H3 antibody ( e , f ) and counterstained with the fluorescent DNA binding dye Hoechst 33342. Cells were then examined using confocal microscopy. The content of immunostained histone H3 was evaluated by SMLM in untreated ( e ) and OND-treated ( f ) HL-1 cells. BrU bromouridine. The error bars represent the standard deviation of three independent samples
    Rna, supplied by Lonza, used in various techniques. Bioz Stars score: 99/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega rna markers
    OND depletes intracellular ATP levels, inhibits transcription, induces relocation of the cellular polyamine pool to the nucleus and reduces the staining density of histone H3 with antibody. a The intracellular concentration of ATP in untreated, OND-exposed and recovering cells was determined using a luciferase dependent assay. b Global rates of transcription, determined by the incorporation of <t>bromouridine</t> into <t>RNA,</t> in untreated cells, cells under OND and cells recovering from OND are presented. HL-1 cells, either untreated or subjected to 1 hour of OND, were fixed, permeabilized and stained either with anti-polyamine antibody ( c , d ) or with anti-total H3 antibody ( e , f ) and counterstained with the fluorescent DNA binding dye Hoechst 33342. Cells were then examined using confocal microscopy. The content of immunostained histone H3 was evaluated by SMLM in untreated ( e ) and OND-treated ( f ) HL-1 cells. BrU bromouridine. The error bars represent the standard deviation of three independent samples
    Rna Markers, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher rna integrity
    OND depletes intracellular ATP levels, inhibits transcription, induces relocation of the cellular polyamine pool to the nucleus and reduces the staining density of histone H3 with antibody. a The intracellular concentration of ATP in untreated, OND-exposed and recovering cells was determined using a luciferase dependent assay. b Global rates of transcription, determined by the incorporation of <t>bromouridine</t> into <t>RNA,</t> in untreated cells, cells under OND and cells recovering from OND are presented. HL-1 cells, either untreated or subjected to 1 hour of OND, were fixed, permeabilized and stained either with anti-polyamine antibody ( c , d ) or with anti-total H3 antibody ( e , f ) and counterstained with the fluorescent DNA binding dye Hoechst 33342. Cells were then examined using confocal microscopy. The content of immunostained histone H3 was evaluated by SMLM in untreated ( e ) and OND-treated ( f ) HL-1 cells. BrU bromouridine. The error bars represent the standard deviation of three independent samples
    Rna Integrity, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6418 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies rna integrity
    <t>Agilent</t> BioAnalyser 2100 traces of total <t>RNA</t> samples. Different volumes of peripheral blood were processed using PAXgene reagent as described in the text. A) Full scale 2.5 mL peripheral blood extraction B) 1.0 mL peripheral blood scaled down extraction C) 0.3 mL peripheral blood scaled down extraction D) Stratagene Universal RNA. The 2.5 mL and 1.0 mL extractions were run on eukaryote total RNA Nano chips and the 0.3 mL extractions and the Universal RNA shown were run on Pico chips. The 18s and 28s RNA peaks can be seen at approximately 42 and 48 seconds respectively.
    Rna Integrity, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 32774 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rna marker
    <t>Agilent</t> BioAnalyser 2100 traces of total <t>RNA</t> samples. Different volumes of peripheral blood were processed using PAXgene reagent as described in the text. A) Full scale 2.5 mL peripheral blood extraction B) 1.0 mL peripheral blood scaled down extraction C) 0.3 mL peripheral blood scaled down extraction D) Stratagene Universal RNA. The 2.5 mL and 1.0 mL extractions were run on eukaryote total RNA Nano chips and the 0.3 mL extractions and the Universal RNA shown were run on Pico chips. The 18s and 28s RNA peaks can be seen at approximately 42 and 48 seconds respectively.
    Rna Marker, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rna  (Qiagen)
    98
    Qiagen rna
    <t>Agilent</t> BioAnalyser 2100 traces of total <t>RNA</t> samples. Different volumes of peripheral blood were processed using PAXgene reagent as described in the text. A) Full scale 2.5 mL peripheral blood extraction B) 1.0 mL peripheral blood scaled down extraction C) 0.3 mL peripheral blood scaled down extraction D) Stratagene Universal RNA. The 2.5 mL and 1.0 mL extractions were run on eukaryote total RNA Nano chips and the 0.3 mL extractions and the Universal RNA shown were run on Pico chips. The 18s and 28s RNA peaks can be seen at approximately 42 and 48 seconds respectively.
    Rna, supplied by Qiagen, used in various techniques. Bioz Stars score: 98/100, based on 49618 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher nanodrop estimation
    <t>Agilent</t> BioAnalyser 2100 traces of total <t>RNA</t> samples. Different volumes of peripheral blood were processed using PAXgene reagent as described in the text. A) Full scale 2.5 mL peripheral blood extraction B) 1.0 mL peripheral blood scaled down extraction C) 0.3 mL peripheral blood scaled down extraction D) Stratagene Universal RNA. The 2.5 mL and 1.0 mL extractions were run on eukaryote total RNA Nano chips and the 0.3 mL extractions and the Universal RNA shown were run on Pico chips. The 18s and 28s RNA peaks can be seen at approximately 42 and 48 seconds respectively.
    Nanodrop Estimation, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher millennium rna markers
    Cultured aorta and vena cava SMC express a single α 1B AR <t>mRNA</t> whereas liver expresses two α 1B AR mRNA. ( A ) Northern analysis using a radiolabeled probe that spanned +93 to +527 was performed on 5 μg (cultured aorta and vena cava SMC) or 2 μg (liver) poly(A) + <t>RNA.</t> ( B ) Sequence of the 5′ region of the α 1B ) (−2,050 to −1,947) and extended it further upstream to −2,460 [all orientations relative to met (ATG) +1].
    Millennium Rna Markers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa nucleospin rna xs
    Cultured aorta and vena cava SMC express a single α 1B AR <t>mRNA</t> whereas liver expresses two α 1B AR mRNA. ( A ) Northern analysis using a radiolabeled probe that spanned +93 to +527 was performed on 5 μg (cultured aorta and vena cava SMC) or 2 μg (liver) poly(A) + <t>RNA.</t> ( B ) Sequence of the 5′ region of the α 1B ) (−2,050 to −1,947) and extended it further upstream to −2,460 [all orientations relative to met (ATG) +1].
    Nucleospin Rna Xs, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    MACHEREY NAGEL nucleospin rna
    Cultured aorta and vena cava SMC express a single α 1B AR <t>mRNA</t> whereas liver expresses two α 1B AR mRNA. ( A ) Northern analysis using a radiolabeled probe that spanned +93 to +527 was performed on 5 μg (cultured aorta and vena cava SMC) or 2 μg (liver) poly(A) + <t>RNA.</t> ( B ) Sequence of the 5′ region of the α 1B ) (−2,050 to −1,947) and extended it further upstream to −2,460 [all orientations relative to met (ATG) +1].
    Nucleospin Rna, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 99/100, based on 808 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Roche rna inhibitor
    Cultured aorta and vena cava SMC express a single α 1B AR <t>mRNA</t> whereas liver expresses two α 1B AR mRNA. ( A ) Northern analysis using a radiolabeled probe that spanned +93 to +527 was performed on 5 μg (cultured aorta and vena cava SMC) or 2 μg (liver) poly(A) + <t>RNA.</t> ( B ) Sequence of the 5′ region of the α 1B ) (−2,050 to −1,947) and extended it further upstream to −2,460 [all orientations relative to met (ATG) +1].
    Rna Inhibitor, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Agilent technologies rna screentape
    Cultured aorta and vena cava SMC express a single α 1B AR <t>mRNA</t> whereas liver expresses two α 1B AR mRNA. ( A ) Northern analysis using a radiolabeled probe that spanned +93 to +527 was performed on 5 μg (cultured aorta and vena cava SMC) or 2 μg (liver) poly(A) + <t>RNA.</t> ( B ) Sequence of the 5′ region of the α 1B ) (−2,050 to −1,947) and extended it further upstream to −2,460 [all orientations relative to met (ATG) +1].
    Rna Screentape, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 295 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    TaKaRa polya rna
    Cultured aorta and vena cava SMC express a single α 1B AR <t>mRNA</t> whereas liver expresses two α 1B AR mRNA. ( A ) Northern analysis using a radiolabeled probe that spanned +93 to +527 was performed on 5 μg (cultured aorta and vena cava SMC) or 2 μg (liver) poly(A) + <t>RNA.</t> ( B ) Sequence of the 5′ region of the α 1B ) (−2,050 to −1,947) and extended it further upstream to −2,460 [all orientations relative to met (ATG) +1].
    Polya Rna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 328 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Agilent technologies rna 6000
    Cultured aorta and vena cava SMC express a single α 1B AR <t>mRNA</t> whereas liver expresses two α 1B AR mRNA. ( A ) Northern analysis using a radiolabeled probe that spanned +93 to +527 was performed on 5 μg (cultured aorta and vena cava SMC) or 2 μg (liver) poly(A) + <t>RNA.</t> ( B ) Sequence of the 5′ region of the α 1B ) (−2,050 to −1,947) and extended it further upstream to −2,460 [all orientations relative to met (ATG) +1].
    Rna 6000, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Norgen Biotek ffpe rna
    Cultured aorta and vena cava SMC express a single α 1B AR <t>mRNA</t> whereas liver expresses two α 1B AR mRNA. ( A ) Northern analysis using a radiolabeled probe that spanned +93 to +527 was performed on 5 μg (cultured aorta and vena cava SMC) or 2 μg (liver) poly(A) + <t>RNA.</t> ( B ) Sequence of the 5′ region of the α 1B ) (−2,050 to −1,947) and extended it further upstream to −2,460 [all orientations relative to met (ATG) +1].
    Ffpe Rna, supplied by Norgen Biotek, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Agilent technologies rna kit
    Cultured aorta and vena cava SMC express a single α 1B AR <t>mRNA</t> whereas liver expresses two α 1B AR mRNA. ( A ) Northern analysis using a radiolabeled probe that spanned +93 to +527 was performed on 5 μg (cultured aorta and vena cava SMC) or 2 μg (liver) poly(A) + <t>RNA.</t> ( B ) Sequence of the 5′ region of the α 1B ) (−2,050 to −1,947) and extended it further upstream to −2,460 [all orientations relative to met (ATG) +1].
    Rna Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 173 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher arraycontrol rna spikes 1
    Cultured aorta and vena cava SMC express a single α 1B AR <t>mRNA</t> whereas liver expresses two α 1B AR mRNA. ( A ) Northern analysis using a radiolabeled probe that spanned +93 to +527 was performed on 5 μg (cultured aorta and vena cava SMC) or 2 μg (liver) poly(A) + <t>RNA.</t> ( B ) Sequence of the 5′ region of the α 1B ) (−2,050 to −1,947) and extended it further upstream to −2,460 [all orientations relative to met (ATG) +1].
    Arraycontrol Rna Spikes 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cultured aorta and vena cava SMC express a single α 1B AR <t>mRNA</t> whereas liver expresses two α 1B AR mRNA. ( A ) Northern analysis using a radiolabeled probe that spanned +93 to +527 was performed on 5 μg (cultured aorta and vena cava SMC) or 2 μg (liver) poly(A) + <t>RNA.</t> ( B ) Sequence of the 5′ region of the α 1B ) (−2,050 to −1,947) and extended it further upstream to −2,460 [all orientations relative to met (ATG) +1].
    Custom Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rna markers
    Northern blot analysis of transcripts of the prophage 3 region. RNAs were prepared from cells grown in sporulation medium at 37°C, separated by electrophoresis, transferred to nitrocellulose membranes, and hybridized with DIG-labeled probe <t>RNA.</t> The RNA samples used were 20-μg samples. The open arrowheads indicate the positions of <t>16S</t> rRNA (1.55 kb) and 23S rRNA (2.93 kb). The solid arrowheads indicate the positions of the transcripts detected, as follows: band A, 7.6 kb; band B, 2.2 kb; band C, 2.9 kb; and band D, 4.2 kb. Panel 1, groEL probe; panel 2, ydiO probe; panel 3, ydiP probe,; panel 4, ydiR probe; panel 5, ydiS probe; panel 6, ydjA probe. The lines on the right in each membrane indicate the positions of molecular weight markers (9, 6, 5, 4, 3, 2.5, 2, 1.5, 1, and 0.5 kb). The T n values below each membrane indicate the numbers of hours (n) after the end of the logarithmic phase of growth.
    Rna Markers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    RT-PCR analysis of PKS gene expression in infected leaves and mycelial cultures. M . fijiensis isolate 10CR1-24 was used to infect banana tissue-culture plants, as well as to inoculate PDB medium. RT-PCR assays for each PKS or hybrid PKS-NRPS were performed on the resulting leaf lesions or mycelium from the PDB liquid culture. Where possible (for all genes except beta-tubulin and PKS10-1 ), primers were designed to span an intron, to distinguish cDNA from any gDNA contamination. Genomic DNA isolated from 10CR1-24 was used as a positive control, and reactions were also done with no added template, as a negative control. Each lane represents RNA isolated from individual infected leaves (left) or mycelium grown in flask culture (right).

    Journal: PLoS ONE

    Article Title: Bioinformatics Prediction of Polyketide Synthase Gene Clusters from Mycosphaerella fijiensis

    doi: 10.1371/journal.pone.0158471

    Figure Lengend Snippet: RT-PCR analysis of PKS gene expression in infected leaves and mycelial cultures. M . fijiensis isolate 10CR1-24 was used to infect banana tissue-culture plants, as well as to inoculate PDB medium. RT-PCR assays for each PKS or hybrid PKS-NRPS were performed on the resulting leaf lesions or mycelium from the PDB liquid culture. Where possible (for all genes except beta-tubulin and PKS10-1 ), primers were designed to span an intron, to distinguish cDNA from any gDNA contamination. Genomic DNA isolated from 10CR1-24 was used as a positive control, and reactions were also done with no added template, as a negative control. Each lane represents RNA isolated from individual infected leaves (left) or mycelium grown in flask culture (right).

    Article Snippet: RNA quality was verified by gel electrophoresis with 300 ng RNA as estimated by Nanodrop (Thermo Scientific). cDNA was synthesized using iScript Select cDNA synthesis kit (Bio-Rad).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Infection, Isolation, Positive Control, Negative Control

    All the cytoplasmic revertants lack the sterility-associated 1.6-kb RNA. Mitochondrial RNAs from “pre-emergent” (A and B) or later “emerging” (C and D) tassels were probed with digoxigenin-labeled orf355 and cox2 probes. ( A ) The 1.6-kb RNA associated with sterility and the non-CMS-associated 2.8-kb RNA (originating from the ψ region) are not detectable in Rev1 and Rev2 when probed with orf355 . A set of larger, ∼5.0, 4.3 and 4.0 kb (arrows), orf355 -containing RNAs is detectable in the revertants. ( B ) A smaller RNA than the 1.6-kb band is detectable in Rev 8, and a slightly larger one is seen in Rev 9. ( C ) Cyt8 does have the orf355 -hybridizing 1.6-kb RNA but it is reduced in amount compared with CMS-S. Cyt8 lacks the 2.8-kb RNA because the ψ region containing the promoter for the 2.8-kb RNA is absent from its genome. Rev3 shows the same set of larger orf355 -containing transcripts as does Rev1. ( D ) The cox2 probe hybridizes to a pair of larger transcripts in Rev1 that are similar in size to the two largest RNAs (∼5.0 and 4.3-kb, arrowheads) detected with orf355 .

    Journal: PLoS ONE

    Article Title: Unique Changes in Mitochondrial Genomes Associated with Reversions of S-Type Cytoplasmic Male Sterility in Maizemar

    doi: 10.1371/journal.pone.0023405

    Figure Lengend Snippet: All the cytoplasmic revertants lack the sterility-associated 1.6-kb RNA. Mitochondrial RNAs from “pre-emergent” (A and B) or later “emerging” (C and D) tassels were probed with digoxigenin-labeled orf355 and cox2 probes. ( A ) The 1.6-kb RNA associated with sterility and the non-CMS-associated 2.8-kb RNA (originating from the ψ region) are not detectable in Rev1 and Rev2 when probed with orf355 . A set of larger, ∼5.0, 4.3 and 4.0 kb (arrows), orf355 -containing RNAs is detectable in the revertants. ( B ) A smaller RNA than the 1.6-kb band is detectable in Rev 8, and a slightly larger one is seen in Rev 9. ( C ) Cyt8 does have the orf355 -hybridizing 1.6-kb RNA but it is reduced in amount compared with CMS-S. Cyt8 lacks the 2.8-kb RNA because the ψ region containing the promoter for the 2.8-kb RNA is absent from its genome. Rev3 shows the same set of larger orf355 -containing transcripts as does Rev1. ( D ) The cox2 probe hybridizes to a pair of larger transcripts in Rev1 that are similar in size to the two largest RNAs (∼5.0 and 4.3-kb, arrowheads) detected with orf355 .

    Article Snippet: Denaturing gels were used for analysis of RNA samples (1.2% agarose and 6% formaldehyde) , and the sizes of the RNAs were estimated by their migrations relative to the bands of the RNA ladder (Invitrogen 0.5–10 kb RNA ladder #15623-200).

    Techniques: Sterility, Labeling

    Expression correlation for ER, PgR, and HER2 genes. Scatterplots reporting the expression correlation of ER, PgR, and HER2 defined by Affymetrix microarray or Illumina RNA-Seq. Each dot is colored according to the corresponding status determined by IHC: green for positive, blue for negative, red for borderline. Spearman correlation coefficient and p-value are provided below the plots.

    Journal: BMC Genomics

    Article Title: Transfer of clinically relevant gene expression signatures in breast cancer: from Affymetrix microarray to Illumina RNA-Sequencing technology

    doi: 10.1186/1471-2164-15-1008

    Figure Lengend Snippet: Expression correlation for ER, PgR, and HER2 genes. Scatterplots reporting the expression correlation of ER, PgR, and HER2 defined by Affymetrix microarray or Illumina RNA-Seq. Each dot is colored according to the corresponding status determined by IHC: green for positive, blue for negative, red for borderline. Spearman correlation coefficient and p-value are provided below the plots.

    Article Snippet: The main aim of the current study is to compare the agreement between two of the most widely used microarray and RNA-Seq platforms, Affymetrix and Illumina HiSeq respectively, in estimating (1) the expression of single genes which are clinically relevant to breast cancer and (2) breast cancer subtype classifiers and gene expression signatures that have been developed over the years with microarrays.

    Techniques: Expressing, Microarray, RNA Sequencing Assay, Immunohistochemistry

    Correlation values for the evaluated subtype classifiers and gene expression signatures. A: Cohen’s Kappa coefficients for subtype classifiers (orange: SCMs; purple: SSPs). B: Spearman correlation values for prognostic (orange), immune (green), stroma (blue) and pathway (purple) signature scores as computed using Affymetrix microarray and Illumina RNA-Seq platforms.

    Journal: BMC Genomics

    Article Title: Transfer of clinically relevant gene expression signatures in breast cancer: from Affymetrix microarray to Illumina RNA-Sequencing technology

    doi: 10.1186/1471-2164-15-1008

    Figure Lengend Snippet: Correlation values for the evaluated subtype classifiers and gene expression signatures. A: Cohen’s Kappa coefficients for subtype classifiers (orange: SCMs; purple: SSPs). B: Spearman correlation values for prognostic (orange), immune (green), stroma (blue) and pathway (purple) signature scores as computed using Affymetrix microarray and Illumina RNA-Seq platforms.

    Article Snippet: The main aim of the current study is to compare the agreement between two of the most widely used microarray and RNA-Seq platforms, Affymetrix and Illumina HiSeq respectively, in estimating (1) the expression of single genes which are clinically relevant to breast cancer and (2) breast cancer subtype classifiers and gene expression signatures that have been developed over the years with microarrays.

    Techniques: Expressing, Microarray, RNA Sequencing Assay

    Gene expression correlation between Affymetrix microarray and Illumina RNA-Seq platforms. A: Expression correlation of the 16,097 genes measured both on Affymetrix microarray and Illumina RNA-Seq platforms after selecting the best Affymetrix probeset using jetset. B: and C: Box plots showing median level of gene expression for both Affymetrix and RNA-Seq for the genes with low (

    Journal: BMC Genomics

    Article Title: Transfer of clinically relevant gene expression signatures in breast cancer: from Affymetrix microarray to Illumina RNA-Sequencing technology

    doi: 10.1186/1471-2164-15-1008

    Figure Lengend Snippet: Gene expression correlation between Affymetrix microarray and Illumina RNA-Seq platforms. A: Expression correlation of the 16,097 genes measured both on Affymetrix microarray and Illumina RNA-Seq platforms after selecting the best Affymetrix probeset using jetset. B: and C: Box plots showing median level of gene expression for both Affymetrix and RNA-Seq for the genes with low (

    Article Snippet: The main aim of the current study is to compare the agreement between two of the most widely used microarray and RNA-Seq platforms, Affymetrix and Illumina HiSeq respectively, in estimating (1) the expression of single genes which are clinically relevant to breast cancer and (2) breast cancer subtype classifiers and gene expression signatures that have been developed over the years with microarrays.

    Techniques: Expressing, Microarray, RNA Sequencing Assay

    OND depletes intracellular ATP levels, inhibits transcription, induces relocation of the cellular polyamine pool to the nucleus and reduces the staining density of histone H3 with antibody. a The intracellular concentration of ATP in untreated, OND-exposed and recovering cells was determined using a luciferase dependent assay. b Global rates of transcription, determined by the incorporation of bromouridine into RNA, in untreated cells, cells under OND and cells recovering from OND are presented. HL-1 cells, either untreated or subjected to 1 hour of OND, were fixed, permeabilized and stained either with anti-polyamine antibody ( c , d ) or with anti-total H3 antibody ( e , f ) and counterstained with the fluorescent DNA binding dye Hoechst 33342. Cells were then examined using confocal microscopy. The content of immunostained histone H3 was evaluated by SMLM in untreated ( e ) and OND-treated ( f ) HL-1 cells. BrU bromouridine. The error bars represent the standard deviation of three independent samples

    Journal: Genome Biology

    Article Title: A transient ischemic environment induces reversible compaction of chromatin

    doi: 10.1186/s13059-015-0802-2

    Figure Lengend Snippet: OND depletes intracellular ATP levels, inhibits transcription, induces relocation of the cellular polyamine pool to the nucleus and reduces the staining density of histone H3 with antibody. a The intracellular concentration of ATP in untreated, OND-exposed and recovering cells was determined using a luciferase dependent assay. b Global rates of transcription, determined by the incorporation of bromouridine into RNA, in untreated cells, cells under OND and cells recovering from OND are presented. HL-1 cells, either untreated or subjected to 1 hour of OND, were fixed, permeabilized and stained either with anti-polyamine antibody ( c , d ) or with anti-total H3 antibody ( e , f ) and counterstained with the fluorescent DNA binding dye Hoechst 33342. Cells were then examined using confocal microscopy. The content of immunostained histone H3 was evaluated by SMLM in untreated ( e ) and OND-treated ( f ) HL-1 cells. BrU bromouridine. The error bars represent the standard deviation of three independent samples

    Article Snippet: Bromouridine labeling of nascent RNA Newly synthesized RNA was pulse labeled by incubating confluent 10-cm culture dishes with 2 mM bromouridine (BrU; Sigma Aldrich) for 1 hour, either under normal culture conditions, under OND, or after 1 hour of recovery from OND.

    Techniques: Staining, Concentration Assay, Luciferase, Binding Assay, Confocal Microscopy, Standard Deviation

    Y3 Competes for HuD Association with Target mRNAs (A) Upper panel: secondary structure of Y3 with HuD interaction sites (visualized with VARNA) based on chemical probing. Center panel: representation of the Y3 “deleted” variant, obtained by eliminating the conserved HuD binding region. Lower panel: His-HA-HuD was induced in NSC-34 cells. Lysates were subjected to RNA pull-downs with biotinylated Y3, followed by immunoblot for HuD and La proteins. Either the wild-type Y3 sequence or the mutant that lacks the HuD binding site was used. (B) Y3 RNA-pull-down showing that HuD interacts with Y3 by the RRM domains, mainly RRM1 and RRM2. (C) Quantification of Y3 and HuD molecule number in NSC-34 cells. The estimated molecule number was calculated by means of a calibration plot generated by known amounts of standards, i.e., in vitro -transcribed (ivt) Y3 RNA and recombinant HuD, respectively. (D) Upper panel: saturation binding curves of recombinant HuD protein as function of increasing amount of RNA probes. Kd values were obtained by non-linear regression analysis. Three independent experiments were performed. Lower panel: AlphaScreen assay using ARE and Y3 RNA probes with lysates of NSC-34 cells expressing HuD protein. Two independent experiments were performed at the hooking point with 50 nM of RNA probes. (E) HuD was induced in NSC-34 cells. Lysates were prepared and RNA pull-downs with biotinylated Y3 were conducted either without (none) or with competitor RNAs included in the extract (7× molar excess). (F) RIP assay of HuD binding to Eef1a1, Eif4a2, and Ncam1 mRNAs after Y3 silencing; data were normalized to Gapdh mRNA levels in each IP. In (D) and (F), data are represented as mean ± SEM t test ∗p

    Journal: Molecular Cell

    Article Title: HuD Is a Neural Translation Enhancer Acting on mTORC1-Responsive Genes and Counteracted by the Y3 Small Non-coding RNA

    doi: 10.1016/j.molcel.2018.06.032

    Figure Lengend Snippet: Y3 Competes for HuD Association with Target mRNAs (A) Upper panel: secondary structure of Y3 with HuD interaction sites (visualized with VARNA) based on chemical probing. Center panel: representation of the Y3 “deleted” variant, obtained by eliminating the conserved HuD binding region. Lower panel: His-HA-HuD was induced in NSC-34 cells. Lysates were subjected to RNA pull-downs with biotinylated Y3, followed by immunoblot for HuD and La proteins. Either the wild-type Y3 sequence or the mutant that lacks the HuD binding site was used. (B) Y3 RNA-pull-down showing that HuD interacts with Y3 by the RRM domains, mainly RRM1 and RRM2. (C) Quantification of Y3 and HuD molecule number in NSC-34 cells. The estimated molecule number was calculated by means of a calibration plot generated by known amounts of standards, i.e., in vitro -transcribed (ivt) Y3 RNA and recombinant HuD, respectively. (D) Upper panel: saturation binding curves of recombinant HuD protein as function of increasing amount of RNA probes. Kd values were obtained by non-linear regression analysis. Three independent experiments were performed. Lower panel: AlphaScreen assay using ARE and Y3 RNA probes with lysates of NSC-34 cells expressing HuD protein. Two independent experiments were performed at the hooking point with 50 nM of RNA probes. (E) HuD was induced in NSC-34 cells. Lysates were prepared and RNA pull-downs with biotinylated Y3 were conducted either without (none) or with competitor RNAs included in the extract (7× molar excess). (F) RIP assay of HuD binding to Eef1a1, Eif4a2, and Ncam1 mRNAs after Y3 silencing; data were normalized to Gapdh mRNA levels in each IP. In (D) and (F), data are represented as mean ± SEM t test ∗p

    Article Snippet: Total RNA was extracted from NSC-34 cells with Trizol (Sigma-Aldrich).

    Techniques: Variant Assay, Binding Assay, Sequencing, Mutagenesis, Generated, In Vitro, Recombinant, Amplified Luminescent Proximity Homogenous Assay, Expressing

    HuD Increases Global and Target-Specific Translation (A) Top enriched Gene Ontology terms among HuD mRNA targets are related to RNA processes, including splicing, transport, stability, and translation (highlighted in bold). (B) Metaprofile of HuD binding sites along protein coding transcripts, showing binding enrichment in 3′UTRs. (C) Right panel: representative sucrose gradient profiles in control and HuD overexpressing NSC-34 cells. Left panel: calculation of the global translation efficiency upon HuD silencing and overexpression. (D) Right: schematic representation of Click-iT AHA assay to quantify de novo protein synthesis in NSC-34 cells. Left: detection of de novo protein synthesis upon HuD silencing and overexpression. Puromycin, a translation inhibitor, was used as negative control. (E) Transcriptome-wide translation efficiency changes upon HuD overexpression in NSC-34 cells. Scatterplot displaying for each gene the average expression signal (CPM) against the log2 change in translation efficiency (delta TE) upon HuD overexpression. Genes with increased or decreased TE are highlighted. (F) Enrichment analysis of HuD RNA targets among genes with increased or decreased TE upon HuD overexpression, compared to enrichments associated with genes changing at either the polysomal or the total RNA level. Fisher’s test ∗ p

    Journal: Molecular Cell

    Article Title: HuD Is a Neural Translation Enhancer Acting on mTORC1-Responsive Genes and Counteracted by the Y3 Small Non-coding RNA

    doi: 10.1016/j.molcel.2018.06.032

    Figure Lengend Snippet: HuD Increases Global and Target-Specific Translation (A) Top enriched Gene Ontology terms among HuD mRNA targets are related to RNA processes, including splicing, transport, stability, and translation (highlighted in bold). (B) Metaprofile of HuD binding sites along protein coding transcripts, showing binding enrichment in 3′UTRs. (C) Right panel: representative sucrose gradient profiles in control and HuD overexpressing NSC-34 cells. Left panel: calculation of the global translation efficiency upon HuD silencing and overexpression. (D) Right: schematic representation of Click-iT AHA assay to quantify de novo protein synthesis in NSC-34 cells. Left: detection of de novo protein synthesis upon HuD silencing and overexpression. Puromycin, a translation inhibitor, was used as negative control. (E) Transcriptome-wide translation efficiency changes upon HuD overexpression in NSC-34 cells. Scatterplot displaying for each gene the average expression signal (CPM) against the log2 change in translation efficiency (delta TE) upon HuD overexpression. Genes with increased or decreased TE are highlighted. (F) Enrichment analysis of HuD RNA targets among genes with increased or decreased TE upon HuD overexpression, compared to enrichments associated with genes changing at either the polysomal or the total RNA level. Fisher’s test ∗ p

    Article Snippet: Total RNA was extracted from NSC-34 cells with Trizol (Sigma-Aldrich).

    Techniques: Binding Assay, Over Expression, Negative Control, Expressing

    HuD Enhancement of Global and Target-Specific Translation Efficiency Does Not Depend on the mTORC1 Pathway (A) Left: western blot analysis of Rps6 and Eif4ebp1 phosphorylation following serum deprivation (8 hr) in NSC-34 cells. (B) Measurement of global TE by sucrose gradient centrifugation in the following conditions: control, starvation, and starvation coupled with HuD overexpression. (C) TE quantification of selected mTOR-responsive mRNAs in control, starvation, and starvation coupled with HuD overexpression conditions. Target-specific TE is the ratio between polysomal and total RNA changes measured by RT-qPCR. Gapdh and Als2 were used as reference genes. (D) Western blot analysis of Eef1a1 and Eif4a3 in NSC-34 cells collected in three different conditions: control, starvation, and starvation with HuD overexpression. (E) Barplot displaying normalized luciferase intensity values in HEK293 cells transiently transfected with HuD, relative to transient transfection of the empty vector. Cells were co-transfected with wild-type (WT) or mutated (MUT) TOP motif bearing luciferase vectors with the 3′UTR of Eef1a1 (HuD target) or Eif4a3 (negative control). In (A)–(E), data are represented as mean ± SEM t test ∗p

    Journal: Molecular Cell

    Article Title: HuD Is a Neural Translation Enhancer Acting on mTORC1-Responsive Genes and Counteracted by the Y3 Small Non-coding RNA

    doi: 10.1016/j.molcel.2018.06.032

    Figure Lengend Snippet: HuD Enhancement of Global and Target-Specific Translation Efficiency Does Not Depend on the mTORC1 Pathway (A) Left: western blot analysis of Rps6 and Eif4ebp1 phosphorylation following serum deprivation (8 hr) in NSC-34 cells. (B) Measurement of global TE by sucrose gradient centrifugation in the following conditions: control, starvation, and starvation coupled with HuD overexpression. (C) TE quantification of selected mTOR-responsive mRNAs in control, starvation, and starvation coupled with HuD overexpression conditions. Target-specific TE is the ratio between polysomal and total RNA changes measured by RT-qPCR. Gapdh and Als2 were used as reference genes. (D) Western blot analysis of Eef1a1 and Eif4a3 in NSC-34 cells collected in three different conditions: control, starvation, and starvation with HuD overexpression. (E) Barplot displaying normalized luciferase intensity values in HEK293 cells transiently transfected with HuD, relative to transient transfection of the empty vector. Cells were co-transfected with wild-type (WT) or mutated (MUT) TOP motif bearing luciferase vectors with the 3′UTR of Eef1a1 (HuD target) or Eif4a3 (negative control). In (A)–(E), data are represented as mean ± SEM t test ∗p

    Article Snippet: Total RNA was extracted from NSC-34 cells with Trizol (Sigma-Aldrich).

    Techniques: Western Blot, Gradient Centrifugation, Over Expression, Quantitative RT-PCR, Luciferase, Transfection, Plasmid Preparation, Negative Control

    Defining the RNA Interaction Landscape of HuD in Motor Neuron Cells (A) Schematic representation of CRAC performed on motor neuron NSC-34 cells. (B) Identification of HuD binding sites from CRAC data. (C) Distribution of HuD PWM scores, calculated from CRAC deletion sites (in violet) and compared with random sequences (in gray). The score threshold to identify bona-fide binding sites was set as the 95th percentile of the random distribution (vertical dashed line). (D) Logo representation of HuD binding sites weighted by binding affinity, calculated as CRAC binding intensities scaled for transcript expression levels. (E) Pie charts displaying the number of HuD RNA targets (upper panel) and the corresponding interaction weight (percentage of CRAC intensity, lower panel) for distinct RNA species. (F) Validation by RNA immunoprecipitation (RIP) and targeted sequencing of 70 HuD targets identified by CRAC. (G) Validation of HuD-Y3 interaction by alternative approaches: left panel, RIP assay followed by Northern blots in HuD transfected NSC-34 cells; right panel, RIP assay followed by RT-qPCR in NSC-34 HuD-inducible cells and in Trex NSC-34 cells (control). In (G), data are represented as mean ± SEM; t test: ∗p

    Journal: Molecular Cell

    Article Title: HuD Is a Neural Translation Enhancer Acting on mTORC1-Responsive Genes and Counteracted by the Y3 Small Non-coding RNA

    doi: 10.1016/j.molcel.2018.06.032

    Figure Lengend Snippet: Defining the RNA Interaction Landscape of HuD in Motor Neuron Cells (A) Schematic representation of CRAC performed on motor neuron NSC-34 cells. (B) Identification of HuD binding sites from CRAC data. (C) Distribution of HuD PWM scores, calculated from CRAC deletion sites (in violet) and compared with random sequences (in gray). The score threshold to identify bona-fide binding sites was set as the 95th percentile of the random distribution (vertical dashed line). (D) Logo representation of HuD binding sites weighted by binding affinity, calculated as CRAC binding intensities scaled for transcript expression levels. (E) Pie charts displaying the number of HuD RNA targets (upper panel) and the corresponding interaction weight (percentage of CRAC intensity, lower panel) for distinct RNA species. (F) Validation by RNA immunoprecipitation (RIP) and targeted sequencing of 70 HuD targets identified by CRAC. (G) Validation of HuD-Y3 interaction by alternative approaches: left panel, RIP assay followed by Northern blots in HuD transfected NSC-34 cells; right panel, RIP assay followed by RT-qPCR in NSC-34 HuD-inducible cells and in Trex NSC-34 cells (control). In (G), data are represented as mean ± SEM; t test: ∗p

    Article Snippet: Total RNA was extracted from NSC-34 cells with Trizol (Sigma-Aldrich).

    Techniques: Binding Assay, Expressing, Immunoprecipitation, Sequencing, Northern Blot, Transfection, Quantitative RT-PCR

    Agilent BioAnalyser 2100 traces of total RNA samples. Different volumes of peripheral blood were processed using PAXgene reagent as described in the text. A) Full scale 2.5 mL peripheral blood extraction B) 1.0 mL peripheral blood scaled down extraction C) 0.3 mL peripheral blood scaled down extraction D) Stratagene Universal RNA. The 2.5 mL and 1.0 mL extractions were run on eukaryote total RNA Nano chips and the 0.3 mL extractions and the Universal RNA shown were run on Pico chips. The 18s and 28s RNA peaks can be seen at approximately 42 and 48 seconds respectively.

    Journal: BMC Immunology

    Article Title: Successful downstream application of the Paxgene Blood RNA system from small blood samples in paediatric patients for quantitative PCR analysis

    doi: 10.1186/1471-2172-8-20

    Figure Lengend Snippet: Agilent BioAnalyser 2100 traces of total RNA samples. Different volumes of peripheral blood were processed using PAXgene reagent as described in the text. A) Full scale 2.5 mL peripheral blood extraction B) 1.0 mL peripheral blood scaled down extraction C) 0.3 mL peripheral blood scaled down extraction D) Stratagene Universal RNA. The 2.5 mL and 1.0 mL extractions were run on eukaryote total RNA Nano chips and the 0.3 mL extractions and the Universal RNA shown were run on Pico chips. The 18s and 28s RNA peaks can be seen at approximately 42 and 48 seconds respectively.

    Article Snippet: RNA integrity was additionally assessed using the Agilent 2100 Bioanalyser [Agilent Technologies].

    Techniques:

    Cultured aorta and vena cava SMC express a single α 1B AR mRNA whereas liver expresses two α 1B AR mRNA. ( A ) Northern analysis using a radiolabeled probe that spanned +93 to +527 was performed on 5 μg (cultured aorta and vena cava SMC) or 2 μg (liver) poly(A) + RNA. ( B ) Sequence of the 5′ region of the α 1B ) (−2,050 to −1,947) and extended it further upstream to −2,460 [all orientations relative to met (ATG) +1].

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Characterization of the ?1B-adrenergic receptor gene promoter region and hypoxia regulatory elements in vascular smooth muscle

    doi:

    Figure Lengend Snippet: Cultured aorta and vena cava SMC express a single α 1B AR mRNA whereas liver expresses two α 1B AR mRNA. ( A ) Northern analysis using a radiolabeled probe that spanned +93 to +527 was performed on 5 μg (cultured aorta and vena cava SMC) or 2 μg (liver) poly(A) + RNA. ( B ) Sequence of the 5′ region of the α 1B ) (−2,050 to −1,947) and extended it further upstream to −2,460 [all orientations relative to met (ATG) +1].

    Article Snippet: Size of mRNA was estimated using RNA Millennium markers (Ambion) and SigmaGel software (Jandel, San Rafael, CA).

    Techniques: Cell Culture, Northern Blot, Sequencing

    Northern blot analysis of transcripts of the prophage 3 region. RNAs were prepared from cells grown in sporulation medium at 37°C, separated by electrophoresis, transferred to nitrocellulose membranes, and hybridized with DIG-labeled probe RNA. The RNA samples used were 20-μg samples. The open arrowheads indicate the positions of 16S rRNA (1.55 kb) and 23S rRNA (2.93 kb). The solid arrowheads indicate the positions of the transcripts detected, as follows: band A, 7.6 kb; band B, 2.2 kb; band C, 2.9 kb; and band D, 4.2 kb. Panel 1, groEL probe; panel 2, ydiO probe; panel 3, ydiP probe,; panel 4, ydiR probe; panel 5, ydiS probe; panel 6, ydjA probe. The lines on the right in each membrane indicate the positions of molecular weight markers (9, 6, 5, 4, 3, 2.5, 2, 1.5, 1, and 0.5 kb). The T n values below each membrane indicate the numbers of hours (n) after the end of the logarithmic phase of growth.

    Journal: Journal of Bacteriology

    Article Title: Molecular Organization of Intrinsic Restriction and Modification Genes BsuM of Bacillus subtilis Marburg

    doi: 10.1128/JB.184.2.381-389.2002

    Figure Lengend Snippet: Northern blot analysis of transcripts of the prophage 3 region. RNAs were prepared from cells grown in sporulation medium at 37°C, separated by electrophoresis, transferred to nitrocellulose membranes, and hybridized with DIG-labeled probe RNA. The RNA samples used were 20-μg samples. The open arrowheads indicate the positions of 16S rRNA (1.55 kb) and 23S rRNA (2.93 kb). The solid arrowheads indicate the positions of the transcripts detected, as follows: band A, 7.6 kb; band B, 2.2 kb; band C, 2.9 kb; and band D, 4.2 kb. Panel 1, groEL probe; panel 2, ydiO probe; panel 3, ydiP probe,; panel 4, ydiR probe; panel 5, ydiS probe; panel 6, ydjA probe. The lines on the right in each membrane indicate the positions of molecular weight markers (9, 6, 5, 4, 3, 2.5, 2, 1.5, 1, and 0.5 kb). The T n values below each membrane indicate the numbers of hours (n) after the end of the logarithmic phase of growth.

    Article Snippet: The length of RNA detected was estimated by calibrating Northern signals with 16S rRNA, 23S rRNA, and RNA markers that were 9, 6, 5, 4, 3, 2.5, 2, 1.5, 1, and 0.5 kb long (Ambion, Inc., Austin, Tex.).

    Techniques: Northern Blot, Electrophoresis, Labeling, Molecular Weight