rna amplification sybr green i Search Results


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  • 99
    Thermo Fisher sybr green i nucleic acid gel stain 10
    Generating a T. brucei ORFeome. A) Assessment of <t>PCR</t> amplification by <t>SYBR</t> Green Relative Fluorescence Units (RFU). Each dot represents an individual PCR reaction, each color represents one of the 21, 384-well plates, graph is of SYBR Relative Fluorescence Units (RFU) vs. 384-well plate position. B) Percent of PCR positive wells (SYBR assessment) for each of the 21, 384-well plates, from the first time amplified. C) Agarose gel bands from each of the 21, 384-well PCR plates pooled prior to gel extraction and cloning, from the first time amplified, compared to 10 Kb ladder DNA ladder. D) ORFeome cloning strategy: attB1 site addition to T. brucei ORFs during PCR amplification, Gateway cloning into pENTR to generate the pENTR ORFeome, and Gateway cloning into T. brucei specific pDEST type vector (pSUN6, shown in detail in SUP. 3) to generate the complete pTrypLib ORFeome.
    Sybr Green I Nucleic Acid Gel Stain 10, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4812 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore sybr green i nucleic acid gel stain
    Loop mediated isothermal amplification of T. foetus DNA. (A) Timecourse of LAMP reaction: lane M, 50-bp ladder; the following lanes show LAMP products at 0, 30, 60, 90 and 120 min. (B) Visual inspection: test tubes under UV light containing the same amplification products as in (A). Positive reactions turned bright green upon addition of <t>SYBR</t> Green I, while the negative ones remained dark under UV light. (C) Digestion of T. foetus LAMP amplification products with PauI (BssHII) developed in 2% agarose gel (left) and silver stained 10% non-denaturing PAGE (right). M, 50-bp ladder; (+) restriction products (1 μl); (−) non digested product (5 μl). Arrowheads point to digestion products with expected sizes of 113 and 135 base pairs.
    Sybr Green I Nucleic Acid Gel Stain, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green i nucleic acid gel stain/product/Millipore
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    94
    Thermo Fisher sybr green i
    SYBRGreen QC. Although the most accurate method to measure cluster density is to perform a first-base incorporation on the flowcell, it is more economical to stain flowcells with <t>SYBRGreen</t> I immediately after amplification, and to examine cluster density qualitatively, using a fluorescence microscope. When coupled with qPCR quantification, this method is usually sufficiently accurate.
    Sybr Green I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 14165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green i/product/Thermo Fisher
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    99
    Thermo Fisher dye sybr green i
    SYBRGreen QC. Although the most accurate method to measure cluster density is to perform a first-base incorporation on the flowcell, it is more economical to stain flowcells with <t>SYBRGreen</t> I immediately after amplification, and to examine cluster density qualitatively, using a fluorescence microscope. When coupled with qPCR quantification, this method is usually sufficiently accurate.
    Dye Sybr Green I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 279 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr green i nucleic acid gel stain
    Optimization of incubation temperature for LAMP reaction. Assessment was based on (A) agarose gel electrophoresis of the LAMP products (B) visualization after addition of <t>SYBR</t> <t>Green</t> I nucleic acid stain into the reaction tubes under UV light exposure, fluorescent green color represents positive amplification; (C) real-time amplification by Genie® III. Here, 1: 66°C, 2: 67° C, 3: 68 °C, 4: 69:° C. 5: 70 °C, 6: 71°C, 7: 72°C and 8: 73°C. Lane M: 100 bp DNA ladder.
    Sybr Green I Nucleic Acid Gel Stain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 314 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green i nucleic acid gel stain/product/Thermo Fisher
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    85
    tiangen biotech co sybr green i rna amplification kit
    Optimization of incubation temperature for LAMP reaction. Assessment was based on (A) agarose gel electrophoresis of the LAMP products (B) visualization after addition of <t>SYBR</t> <t>Green</t> I nucleic acid stain into the reaction tubes under UV light exposure, fluorescent green color represents positive amplification; (C) real-time amplification by Genie® III. Here, 1: 66°C, 2: 67° C, 3: 68 °C, 4: 69:° C. 5: 70 °C, 6: 71°C, 7: 72°C and 8: 73°C. Lane M: 100 bp DNA ladder.
    Sybr Green I Rna Amplification Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher dna dye
    Optimization of incubation temperature for LAMP reaction. Assessment was based on (A) agarose gel electrophoresis of the LAMP products (B) visualization after addition of <t>SYBR</t> <t>Green</t> I nucleic acid stain into the reaction tubes under UV light exposure, fluorescent green color represents positive amplification; (C) real-time amplification by Genie® III. Here, 1: 66°C, 2: 67° C, 3: 68 °C, 4: 69:° C. 5: 70 °C, 6: 71°C, 7: 72°C and 8: 73°C. Lane M: 100 bp DNA ladder.
    Dna Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 264 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher mastermix
    Optimization of incubation temperature for LAMP reaction. Assessment was based on (A) agarose gel electrophoresis of the LAMP products (B) visualization after addition of <t>SYBR</t> <t>Green</t> I nucleic acid stain into the reaction tubes under UV light exposure, fluorescent green color represents positive amplification; (C) real-time amplification by Genie® III. Here, 1: 66°C, 2: 67° C, 3: 68 °C, 4: 69:° C. 5: 70 °C, 6: 71°C, 7: 72°C and 8: 73°C. Lane M: 100 bp DNA ladder.
    Mastermix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2391 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Generating a T. brucei ORFeome. A) Assessment of PCR amplification by SYBR Green Relative Fluorescence Units (RFU). Each dot represents an individual PCR reaction, each color represents one of the 21, 384-well plates, graph is of SYBR Relative Fluorescence Units (RFU) vs. 384-well plate position. B) Percent of PCR positive wells (SYBR assessment) for each of the 21, 384-well plates, from the first time amplified. C) Agarose gel bands from each of the 21, 384-well PCR plates pooled prior to gel extraction and cloning, from the first time amplified, compared to 10 Kb ladder DNA ladder. D) ORFeome cloning strategy: attB1 site addition to T. brucei ORFs during PCR amplification, Gateway cloning into pENTR to generate the pENTR ORFeome, and Gateway cloning into T. brucei specific pDEST type vector (pSUN6, shown in detail in SUP. 3) to generate the complete pTrypLib ORFeome.

    Journal: bioRxiv

    Article Title: A Trypanosoma brucei ORFeome-based Gain-of-Function Library reveals novel genes associated with melarsoprol resistance

    doi: 10.1101/849042

    Figure Lengend Snippet: Generating a T. brucei ORFeome. A) Assessment of PCR amplification by SYBR Green Relative Fluorescence Units (RFU). Each dot represents an individual PCR reaction, each color represents one of the 21, 384-well plates, graph is of SYBR Relative Fluorescence Units (RFU) vs. 384-well plate position. B) Percent of PCR positive wells (SYBR assessment) for each of the 21, 384-well plates, from the first time amplified. C) Agarose gel bands from each of the 21, 384-well PCR plates pooled prior to gel extraction and cloning, from the first time amplified, compared to 10 Kb ladder DNA ladder. D) ORFeome cloning strategy: attB1 site addition to T. brucei ORFs during PCR amplification, Gateway cloning into pENTR to generate the pENTR ORFeome, and Gateway cloning into T. brucei specific pDEST type vector (pSUN6, shown in detail in SUP. 3) to generate the complete pTrypLib ORFeome.

    Article Snippet: Following PCR reaction completion SYBR Green I (Invitrogen S-7563) was added to each well at 1:10 PCR volume for a final concentration of 10x SYBR and relative fluorescence units (RFU) were measured on a BioTek Synergy H4 Hybrid Multi-Mode Microplate reader in comparison to a standard dilution of known DNA concentration.

    Techniques: Polymerase Chain Reaction, Amplification, SYBR Green Assay, Fluorescence, Agarose Gel Electrophoresis, Gel Extraction, Clone Assay, Plasmid Preparation

    Loop mediated isothermal amplification of T. foetus DNA. (A) Timecourse of LAMP reaction: lane M, 50-bp ladder; the following lanes show LAMP products at 0, 30, 60, 90 and 120 min. (B) Visual inspection: test tubes under UV light containing the same amplification products as in (A). Positive reactions turned bright green upon addition of SYBR Green I, while the negative ones remained dark under UV light. (C) Digestion of T. foetus LAMP amplification products with PauI (BssHII) developed in 2% agarose gel (left) and silver stained 10% non-denaturing PAGE (right). M, 50-bp ladder; (+) restriction products (1 μl); (−) non digested product (5 μl). Arrowheads point to digestion products with expected sizes of 113 and 135 base pairs.

    Journal: Veterinary Parasitology

    Article Title: Loop mediated isothermal amplification of 5.8S rDNA for specific detection of Tritrichomonas foetus

    doi: 10.1016/j.vetpar.2012.11.034

    Figure Lengend Snippet: Loop mediated isothermal amplification of T. foetus DNA. (A) Timecourse of LAMP reaction: lane M, 50-bp ladder; the following lanes show LAMP products at 0, 30, 60, 90 and 120 min. (B) Visual inspection: test tubes under UV light containing the same amplification products as in (A). Positive reactions turned bright green upon addition of SYBR Green I, while the negative ones remained dark under UV light. (C) Digestion of T. foetus LAMP amplification products with PauI (BssHII) developed in 2% agarose gel (left) and silver stained 10% non-denaturing PAGE (right). M, 50-bp ladder; (+) restriction products (1 μl); (−) non digested product (5 μl). Arrowheads point to digestion products with expected sizes of 113 and 135 base pairs.

    Article Snippet: For visual inspection, 2 μL of 1/100 dilution of SYBR Green I (S9430, Sigma–Aldrich) were added to 25 μL LAMP reactions.

    Techniques: Amplification, SYBR Green Assay, Agarose Gel Electrophoresis, Staining, Polyacrylamide Gel Electrophoresis

    Specificity of T. foetus LAMP. (A) DNA from Trichomonads strains were amplified through PCR (primers TFR1 and TFR2 for genus recognition) and developed on 5% non-denaturing PAGE. Lane M, 50-bp ladder used as a size marker; lanes 1, 2, 3 and 4 Tetratrichomonas spp . strains GM018, GM019, GM020 and GM022 respectively; lanes 5, 6, 12, 13 and 14 T. foetus strains GM031, GM032, GM050, GM051 and GM052 respectively; lanes 7–10 Pentatrichomonas hominis strains GM033, GM034, GM035 and GM036 respectively; lane 11 mixed culture of Tetratrichomonas spp . GM022 and Pentatrichomonas hominis GM035. (B) The same samples as in (A) but with primers TFR3 and TFR4 (specific for T. foetus ). (C) Agarose gel analysis of LAMP specific for T. foetus 5.8S ribosomal gene. Lanes follow the same order as in (A) and (B). (D) LAMP reactions as in (C) after addition of SYBR green I.

    Journal: Veterinary Parasitology

    Article Title: Loop mediated isothermal amplification of 5.8S rDNA for specific detection of Tritrichomonas foetus

    doi: 10.1016/j.vetpar.2012.11.034

    Figure Lengend Snippet: Specificity of T. foetus LAMP. (A) DNA from Trichomonads strains were amplified through PCR (primers TFR1 and TFR2 for genus recognition) and developed on 5% non-denaturing PAGE. Lane M, 50-bp ladder used as a size marker; lanes 1, 2, 3 and 4 Tetratrichomonas spp . strains GM018, GM019, GM020 and GM022 respectively; lanes 5, 6, 12, 13 and 14 T. foetus strains GM031, GM032, GM050, GM051 and GM052 respectively; lanes 7–10 Pentatrichomonas hominis strains GM033, GM034, GM035 and GM036 respectively; lane 11 mixed culture of Tetratrichomonas spp . GM022 and Pentatrichomonas hominis GM035. (B) The same samples as in (A) but with primers TFR3 and TFR4 (specific for T. foetus ). (C) Agarose gel analysis of LAMP specific for T. foetus 5.8S ribosomal gene. Lanes follow the same order as in (A) and (B). (D) LAMP reactions as in (C) after addition of SYBR green I.

    Article Snippet: For visual inspection, 2 μL of 1/100 dilution of SYBR Green I (S9430, Sigma–Aldrich) were added to 25 μL LAMP reactions.

    Techniques: Amplification, Polymerase Chain Reaction, Polyacrylamide Gel Electrophoresis, Marker, Agarose Gel Electrophoresis, SYBR Green Assay

    Specificity test for the real-time PCR assay developed for the identification of M. yongonense with using 28 reference strains of Mycobacterium species. M. yongonense was specifically identified based on Cq and melting temperature measurements. The tested strains are the same as those listed in Additional file 5 and were tested in duplicate via SYBR Green I real-time PCR. a , amplification curves; b , melting curve analysis

    Journal: BMC Genomics

    Article Title: Identification of ISMyo2, a novel insertion sequence element of IS21 family and its diagnostic potential for detection of Mycobacterium yongonense

    doi: 10.1186/s12864-015-1978-2

    Figure Lengend Snippet: Specificity test for the real-time PCR assay developed for the identification of M. yongonense with using 28 reference strains of Mycobacterium species. M. yongonense was specifically identified based on Cq and melting temperature measurements. The tested strains are the same as those listed in Additional file 5 and were tested in duplicate via SYBR Green I real-time PCR. a , amplification curves; b , melting curve analysis

    Article Snippet: A 10 μl reaction mixture was prepared for each sample, with the following components: 1 μl of Taq buffer (including 20 mM MgCl2 ) supplied together with Ex Taq HS (Takara), 0.25 μM forward primer, 0.25 μM reverse primer, 0.2 mM dNTPs, 0.7 mg/ml BSA (NEB), 0.5 × SYBR Green I (Sigma S9430), 3 % DMSO, 0.25 U of ExTaq HS, and sterile distilled water.

    Techniques: Real-time Polymerase Chain Reaction, SYBR Green Assay, Amplification

    SYBRGreen QC. Although the most accurate method to measure cluster density is to perform a first-base incorporation on the flowcell, it is more economical to stain flowcells with SYBRGreen I immediately after amplification, and to examine cluster density qualitatively, using a fluorescence microscope. When coupled with qPCR quantification, this method is usually sufficiently accurate.

    Journal: Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]

    Article Title: Improved Protocols for Illumina Sequencing

    doi: 10.1002/0471142905.hg1802s62

    Figure Lengend Snippet: SYBRGreen QC. Although the most accurate method to measure cluster density is to perform a first-base incorporation on the flowcell, it is more economical to stain flowcells with SYBRGreen I immediately after amplification, and to examine cluster density qualitatively, using a fluorescence microscope. When coupled with qPCR quantification, this method is usually sufficiently accurate.

    Article Snippet: ) Sodium ascorbate (Sigma, cat. no. A4034) 10,000× SYBRGreen I (Life Technologies, cat. no. S-7567) Amplified flowcell PR2 buffer (Illumina, supplied with sequencing kits) 15-ml Falcon tubes 0.2-μm syringe filter cBot (Illumina) Fluorescence microscope, set up to detect SYBRGreen I Prepare 5 ml of a solution of 0.1 M Tris·HCl, pH 8.0, and 0.1 mM sodium ascorbate, and filter into a 15-ml Falcon tube using a 0.2-μm syringe filter.

    Techniques: Staining, Amplification, Fluorescence, Microscopy, Real-time Polymerase Chain Reaction

    Optimization of incubation temperature for LAMP reaction. Assessment was based on (A) agarose gel electrophoresis of the LAMP products (B) visualization after addition of SYBR Green I nucleic acid stain into the reaction tubes under UV light exposure, fluorescent green color represents positive amplification; (C) real-time amplification by Genie® III. Here, 1: 66°C, 2: 67° C, 3: 68 °C, 4: 69:° C. 5: 70 °C, 6: 71°C, 7: 72°C and 8: 73°C. Lane M: 100 bp DNA ladder.

    Journal: bioRxiv

    Article Title: Rapid detection of Pecan Root-Knot Nematode, Meloidogyne partityla, in laboratory and field conditions using loop-mediated isothermal amplification

    doi: 10.1101/2020.01.09.900076

    Figure Lengend Snippet: Optimization of incubation temperature for LAMP reaction. Assessment was based on (A) agarose gel electrophoresis of the LAMP products (B) visualization after addition of SYBR Green I nucleic acid stain into the reaction tubes under UV light exposure, fluorescent green color represents positive amplification; (C) real-time amplification by Genie® III. Here, 1: 66°C, 2: 67° C, 3: 68 °C, 4: 69:° C. 5: 70 °C, 6: 71°C, 7: 72°C and 8: 73°C. Lane M: 100 bp DNA ladder.

    Article Snippet: Along with the gel electrophoresis and SYBR™ Green I nucleic acid gel stain, less time was taken for amplification at that particular temperature during amplification using Genie® III.

    Techniques: Incubation, Agarose Gel Electrophoresis, SYBR Green Assay, Staining, Amplification

    Loop-mediated isothermal amplification of DNA from four pure culture Meloidogyne partityla. Assessment was based on (A) agarose gel electrophoresis of the LAMP products; (B) visualization after addition of SYBR Green I nucleic acid stain into the reaction tubes under UV light exposure, fluorescent green color represents positive amplification; (C) real-time amplification by Genie® III; (D) PCR amplification using primer pair C2F3/1108. Lane M: 100 bp DNA ladder; Mp1, Mp2, Mp3 and Mp4: four different isolates of Meloidogyne partityla ; Neg: negative control.

    Journal: bioRxiv

    Article Title: Rapid detection of Pecan Root-Knot Nematode, Meloidogyne partityla, in laboratory and field conditions using loop-mediated isothermal amplification

    doi: 10.1101/2020.01.09.900076

    Figure Lengend Snippet: Loop-mediated isothermal amplification of DNA from four pure culture Meloidogyne partityla. Assessment was based on (A) agarose gel electrophoresis of the LAMP products; (B) visualization after addition of SYBR Green I nucleic acid stain into the reaction tubes under UV light exposure, fluorescent green color represents positive amplification; (C) real-time amplification by Genie® III; (D) PCR amplification using primer pair C2F3/1108. Lane M: 100 bp DNA ladder; Mp1, Mp2, Mp3 and Mp4: four different isolates of Meloidogyne partityla ; Neg: negative control.

    Article Snippet: Along with the gel electrophoresis and SYBR™ Green I nucleic acid gel stain, less time was taken for amplification at that particular temperature during amplification using Genie® III.

    Techniques: Amplification, Agarose Gel Electrophoresis, SYBR Green Assay, Staining, Polymerase Chain Reaction, Negative Control

    Sensitivity of LAMP assay using 10-fold serially diluted DNA extracted from pureculture of Meloidogyne partityla . Assessment was based on (A) agarose gel electrophoresis of the LAMP products (B) visualization after addition of SYBR Green I nucleic acid stain into the reaction tubes under UV light exposure, fluorescent green color represents positive amplification; (C) real-timeamplificationby Genie Ill (D) PCR amplification using primer pair C2F3/1108. Lane M: 100 bp DNA ladder; numbers 1 to 7: 10-fold serial dilution of M. partityla DNA from 100 ng/µl to 10-4 ng/µl; Neg: negative control.

    Journal: bioRxiv

    Article Title: Rapid detection of Pecan Root-Knot Nematode, Meloidogyne partityla, in laboratory and field conditions using loop-mediated isothermal amplification

    doi: 10.1101/2020.01.09.900076

    Figure Lengend Snippet: Sensitivity of LAMP assay using 10-fold serially diluted DNA extracted from pureculture of Meloidogyne partityla . Assessment was based on (A) agarose gel electrophoresis of the LAMP products (B) visualization after addition of SYBR Green I nucleic acid stain into the reaction tubes under UV light exposure, fluorescent green color represents positive amplification; (C) real-timeamplificationby Genie Ill (D) PCR amplification using primer pair C2F3/1108. Lane M: 100 bp DNA ladder; numbers 1 to 7: 10-fold serial dilution of M. partityla DNA from 100 ng/µl to 10-4 ng/µl; Neg: negative control.

    Article Snippet: Along with the gel electrophoresis and SYBR™ Green I nucleic acid gel stain, less time was taken for amplification at that particular temperature during amplification using Genie® III.

    Techniques: Lamp Assay, Agarose Gel Electrophoresis, SYBR Green Assay, Staining, Amplification, Polymerase Chain Reaction, Serial Dilution, Negative Control

    Specificity determination of LAMP assay using DNA from pure cultures of five different Meloidogyne spp. Assessment was based on (A) agarose gel electrophoresis of the LAMP products; (B) visualization after addition of SYBR Green I nucleic acid stain into the reaction tubes under UV light exposure, fluorescent green color represents positive amplification (C) real-time amplification by Genie III. Lane M: 100 bp DNA ladder;M. part; Meloidogyne partityla ; M. hap: Meloidogyne hapla ; M. jav: Meloidogyne javanica; M. inc: Meloidogyne incognita ; M. are: Meloidogyne arenaria; Neg: negative control.

    Journal: bioRxiv

    Article Title: Rapid detection of Pecan Root-Knot Nematode, Meloidogyne partityla, in laboratory and field conditions using loop-mediated isothermal amplification

    doi: 10.1101/2020.01.09.900076

    Figure Lengend Snippet: Specificity determination of LAMP assay using DNA from pure cultures of five different Meloidogyne spp. Assessment was based on (A) agarose gel electrophoresis of the LAMP products; (B) visualization after addition of SYBR Green I nucleic acid stain into the reaction tubes under UV light exposure, fluorescent green color represents positive amplification (C) real-time amplification by Genie III. Lane M: 100 bp DNA ladder;M. part; Meloidogyne partityla ; M. hap: Meloidogyne hapla ; M. jav: Meloidogyne javanica; M. inc: Meloidogyne incognita ; M. are: Meloidogyne arenaria; Neg: negative control.

    Article Snippet: Along with the gel electrophoresis and SYBR™ Green I nucleic acid gel stain, less time was taken for amplification at that particular temperature during amplification using Genie® III.

    Techniques: Lamp Assay, Agarose Gel Electrophoresis, SYBR Green Assay, Staining, Amplification, Negative Control

    Representative analytical PCR of size-selected RRBS libraries.The 160–340 bp size-selected RRBS libraries (represented by a, b, and c) were amplified with either 15 or 20 cycles of PCR to determine the optimal number of cycles for large-scale amplification. PCR products were visualized on a 4–20% Criterion gradient polyacrylamide TBE gel stained with SYBR green nucleic acid gel stain alongside a 25 bp DNA ladder. For these libraries, 13 (a) and 14 (b and c) cycles were chosen for large-scale amplification. The distinct band at 125 bp in all libraries was possibly due to adaptor-adaptor dimerization.

    Journal: Journal of Biomedicine and Biotechnology

    Article Title: Technical Considerations for Reduced Representation Bisulfite Sequencing with Multiplexed Libraries

    doi: 10.1155/2012/741542

    Figure Lengend Snippet: Representative analytical PCR of size-selected RRBS libraries.The 160–340 bp size-selected RRBS libraries (represented by a, b, and c) were amplified with either 15 or 20 cycles of PCR to determine the optimal number of cycles for large-scale amplification. PCR products were visualized on a 4–20% Criterion gradient polyacrylamide TBE gel stained with SYBR green nucleic acid gel stain alongside a 25 bp DNA ladder. For these libraries, 13 (a) and 14 (b and c) cycles were chosen for large-scale amplification. The distinct band at 125 bp in all libraries was possibly due to adaptor-adaptor dimerization.

    Article Snippet: Libraries were amplified with 15–18 cycles of amplification and then visualized on a 4–20% Criterion gradient polyacrylamide TBE gel (BioRad Laboratories, Hercules, CA) stained with SYBR green nucleic acid gel stain (Life Technologies, Grand Island, NY).

    Techniques: Polymerase Chain Reaction, Amplification, Staining, SYBR Green Assay