rna Thermo Fisher Search Results


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  • 93
    Millipore rna
    Rna, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 5994 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher ribonucleic acid sirna
    <t>Caspase-14</t> modulates trophoblast gene expression . The effect of caspase-14 <t>siRNA</t> on the expression of (A-C) hCG, (D-F) KLF4 and (G-I) cytokeratin-18 in DMSO-treated BeWo cells. * = P
    Ribonucleic Acid Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher ribonucleic acids sirnas
    <t>Caspase-14</t> modulates trophoblast gene expression . The effect of caspase-14 <t>siRNA</t> on the expression of (A-C) hCG, (D-F) KLF4 and (G-I) cytokeratin-18 in DMSO-treated BeWo cells. * = P
    Ribonucleic Acids Sirnas, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    76
    Thermo Fisher ribonucleic acid sirna oligonucleotides
    <t>Caspase-14</t> modulates trophoblast gene expression . The effect of caspase-14 <t>siRNA</t> on the expression of (A-C) hCG, (D-F) KLF4 and (G-I) cytokeratin-18 in DMSO-treated BeWo cells. * = P
    Ribonucleic Acid Sirna Oligonucleotides, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Thermo Fisher real time pcr whole ribonucleic acid rna
    <t>Caspase-14</t> modulates trophoblast gene expression . The effect of caspase-14 <t>siRNA</t> on the expression of (A-C) hCG, (D-F) KLF4 and (G-I) cytokeratin-18 in DMSO-treated BeWo cells. * = P
    Real Time Pcr Whole Ribonucleic Acid Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Thermo Fisher stealth ribonucleic acid rna interference
    Effects of B7-H3 on the expression of VEGF in MCF-7 cells. Notes: ( A ) Expression level of VEGF-C mRNA was measured by RT-PCR. ( B ) Expression level of VEGF-C mRNA was measured by means of real-time PCR for 48 hours. ( C ) The secretion of VEGF in serum of MCF-7 cell line after siRNA transfection. Abbreviations: VEGF, vascular endothelial growth factor; mRNA, messenger ribonucleic acid; RT-PCR, reverse-transcription polymerase chain reaction; siRNA, short interfering ribonucleic acid; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; <t>RNA,</t> ribonucleic acid; <t>RNAi,</t> ribonucleic acid interference.
    Stealth Ribonucleic Acid Rna Interference, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Thermo Fisher ribonucleic acid
    Effects of B7-H3 on the expression of VEGF in MCF-7 cells. Notes: ( A ) Expression level of VEGF-C mRNA was measured by RT-PCR. ( B ) Expression level of VEGF-C mRNA was measured by means of real-time PCR for 48 hours. ( C ) The secretion of VEGF in serum of MCF-7 cell line after siRNA transfection. Abbreviations: VEGF, vascular endothelial growth factor; mRNA, messenger ribonucleic acid; RT-PCR, reverse-transcription polymerase chain reaction; siRNA, short interfering ribonucleic acid; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; <t>RNA,</t> ribonucleic acid; <t>RNAi,</t> ribonucleic acid interference.
    Ribonucleic Acid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher ribonucleic acid rna isolation kit
    Effects of B7-H3 on the expression of VEGF in MCF-7 cells. Notes: ( A ) Expression level of VEGF-C mRNA was measured by RT-PCR. ( B ) Expression level of VEGF-C mRNA was measured by means of real-time PCR for 48 hours. ( C ) The secretion of VEGF in serum of MCF-7 cell line after siRNA transfection. Abbreviations: VEGF, vascular endothelial growth factor; mRNA, messenger ribonucleic acid; RT-PCR, reverse-transcription polymerase chain reaction; siRNA, short interfering ribonucleic acid; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; <t>RNA,</t> ribonucleic acid; <t>RNAi,</t> ribonucleic acid interference.
    Ribonucleic Acid Rna Isolation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher magmax pathogen ribonucleic acid dna kit
    Effects of B7-H3 on the expression of VEGF in MCF-7 cells. Notes: ( A ) Expression level of VEGF-C mRNA was measured by RT-PCR. ( B ) Expression level of VEGF-C mRNA was measured by means of real-time PCR for 48 hours. ( C ) The secretion of VEGF in serum of MCF-7 cell line after siRNA transfection. Abbreviations: VEGF, vascular endothelial growth factor; mRNA, messenger ribonucleic acid; RT-PCR, reverse-transcription polymerase chain reaction; siRNA, short interfering ribonucleic acid; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; <t>RNA,</t> ribonucleic acid; <t>RNAi,</t> ribonucleic acid interference.
    Magmax Pathogen Ribonucleic Acid Dna Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher pre micro ribonucleic acid
    Effects of B7-H3 on the expression of VEGF in MCF-7 cells. Notes: ( A ) Expression level of VEGF-C mRNA was measured by RT-PCR. ( B ) Expression level of VEGF-C mRNA was measured by means of real-time PCR for 48 hours. ( C ) The secretion of VEGF in serum of MCF-7 cell line after siRNA transfection. Abbreviations: VEGF, vascular endothelial growth factor; mRNA, messenger ribonucleic acid; RT-PCR, reverse-transcription polymerase chain reaction; siRNA, short interfering ribonucleic acid; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; <t>RNA,</t> ribonucleic acid; <t>RNAi,</t> ribonucleic acid interference.
    Pre Micro Ribonucleic Acid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    76
    Thermo Fisher human lung ribonucleic acid rna
    Effects of B7-H3 on the expression of VEGF in MCF-7 cells. Notes: ( A ) Expression level of VEGF-C mRNA was measured by RT-PCR. ( B ) Expression level of VEGF-C mRNA was measured by means of real-time PCR for 48 hours. ( C ) The secretion of VEGF in serum of MCF-7 cell line after siRNA transfection. Abbreviations: VEGF, vascular endothelial growth factor; mRNA, messenger ribonucleic acid; RT-PCR, reverse-transcription polymerase chain reaction; siRNA, short interfering ribonucleic acid; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; <t>RNA,</t> ribonucleic acid; <t>RNAi,</t> ribonucleic acid interference.
    Human Lung Ribonucleic Acid Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Thermo Fisher ribonucleic acid rna stabilization solution
    Effects of B7-H3 on the expression of VEGF in MCF-7 cells. Notes: ( A ) Expression level of VEGF-C mRNA was measured by RT-PCR. ( B ) Expression level of VEGF-C mRNA was measured by means of real-time PCR for 48 hours. ( C ) The secretion of VEGF in serum of MCF-7 cell line after siRNA transfection. Abbreviations: VEGF, vascular endothelial growth factor; mRNA, messenger ribonucleic acid; RT-PCR, reverse-transcription polymerase chain reaction; siRNA, short interfering ribonucleic acid; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; <t>RNA,</t> ribonucleic acid; <t>RNAi,</t> ribonucleic acid interference.
    Ribonucleic Acid Rna Stabilization Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    70
    Fisher Scientific ngs thermo fisher scientific
    Effects of B7-H3 on the expression of VEGF in MCF-7 cells. Notes: ( A ) Expression level of VEGF-C mRNA was measured by RT-PCR. ( B ) Expression level of VEGF-C mRNA was measured by means of real-time PCR for 48 hours. ( C ) The secretion of VEGF in serum of MCF-7 cell line after siRNA transfection. Abbreviations: VEGF, vascular endothelial growth factor; mRNA, messenger ribonucleic acid; RT-PCR, reverse-transcription polymerase chain reaction; siRNA, short interfering ribonucleic acid; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; <t>RNA,</t> ribonucleic acid; <t>RNAi,</t> ribonucleic acid interference.
    Ngs Thermo Fisher Scientific, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 70/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Fisher Scientific dharmacon thermo fisher scientific
    Effects of B7-H3 on the expression of VEGF in MCF-7 cells. Notes: ( A ) Expression level of VEGF-C mRNA was measured by RT-PCR. ( B ) Expression level of VEGF-C mRNA was measured by means of real-time PCR for 48 hours. ( C ) The secretion of VEGF in serum of MCF-7 cell line after siRNA transfection. Abbreviations: VEGF, vascular endothelial growth factor; mRNA, messenger ribonucleic acid; RT-PCR, reverse-transcription polymerase chain reaction; siRNA, short interfering ribonucleic acid; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; <t>RNA,</t> ribonucleic acid; <t>RNAi,</t> ribonucleic acid interference.
    Dharmacon Thermo Fisher Scientific, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 85/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Thermo Fisher gapdh
    Effects of B7-H3 on the expression of VEGF in MCF-7 cells. Notes: ( A ) Expression level of VEGF-C mRNA was measured by RT-PCR. ( B ) Expression level of VEGF-C mRNA was measured by means of real-time PCR for 48 hours. ( C ) The secretion of VEGF in serum of MCF-7 cell line after siRNA transfection. Abbreviations: VEGF, vascular endothelial growth factor; mRNA, messenger ribonucleic acid; RT-PCR, reverse-transcription polymerase chain reaction; siRNA, short interfering ribonucleic acid; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; <t>RNA,</t> ribonucleic acid; <t>RNAi,</t> ribonucleic acid interference.
    Gapdh, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 7920 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Thermo Fisher thermo fisher scientific open biosystems
    Effects of B7-H3 on the expression of VEGF in MCF-7 cells. Notes: ( A ) Expression level of VEGF-C mRNA was measured by RT-PCR. ( B ) Expression level of VEGF-C mRNA was measured by means of real-time PCR for 48 hours. ( C ) The secretion of VEGF in serum of MCF-7 cell line after siRNA transfection. Abbreviations: VEGF, vascular endothelial growth factor; mRNA, messenger ribonucleic acid; RT-PCR, reverse-transcription polymerase chain reaction; siRNA, short interfering ribonucleic acid; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; <t>RNA,</t> ribonucleic acid; <t>RNAi,</t> ribonucleic acid interference.
    Thermo Fisher Scientific Open Biosystems, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Thermo Fisher thermo fisher trizol reagent
    Effects of B7-H3 on the expression of VEGF in MCF-7 cells. Notes: ( A ) Expression level of VEGF-C mRNA was measured by RT-PCR. ( B ) Expression level of VEGF-C mRNA was measured by means of real-time PCR for 48 hours. ( C ) The secretion of VEGF in serum of MCF-7 cell line after siRNA transfection. Abbreviations: VEGF, vascular endothelial growth factor; mRNA, messenger ribonucleic acid; RT-PCR, reverse-transcription polymerase chain reaction; siRNA, short interfering ribonucleic acid; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; <t>RNA,</t> ribonucleic acid; <t>RNAi,</t> ribonucleic acid interference.
    Thermo Fisher Trizol Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Thermo Fisher thermo fisher scientific edta
    Effects of B7-H3 on the expression of VEGF in MCF-7 cells. Notes: ( A ) Expression level of VEGF-C mRNA was measured by RT-PCR. ( B ) Expression level of VEGF-C mRNA was measured by means of real-time PCR for 48 hours. ( C ) The secretion of VEGF in serum of MCF-7 cell line after siRNA transfection. Abbreviations: VEGF, vascular endothelial growth factor; mRNA, messenger ribonucleic acid; RT-PCR, reverse-transcription polymerase chain reaction; siRNA, short interfering ribonucleic acid; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; <t>RNA,</t> ribonucleic acid; <t>RNAi,</t> ribonucleic acid interference.
    Thermo Fisher Scientific Edta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Thermo Fisher arcturus picopure rna isolation kit thermo fisher scientific waltham
    Effects of B7-H3 on the expression of VEGF in MCF-7 cells. Notes: ( A ) Expression level of VEGF-C mRNA was measured by RT-PCR. ( B ) Expression level of VEGF-C mRNA was measured by means of real-time PCR for 48 hours. ( C ) The secretion of VEGF in serum of MCF-7 cell line after siRNA transfection. Abbreviations: VEGF, vascular endothelial growth factor; mRNA, messenger ribonucleic acid; RT-PCR, reverse-transcription polymerase chain reaction; siRNA, short interfering ribonucleic acid; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; <t>RNA,</t> ribonucleic acid; <t>RNAi,</t> ribonucleic acid interference.
    Arcturus Picopure Rna Isolation Kit Thermo Fisher Scientific Waltham, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 80 stars, based on 8 article reviews
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    90
    Thermo Fisher ribosomal ribonucleic acid
    Effects of B7-H3 on the expression of VEGF in MCF-7 cells. Notes: ( A ) Expression level of VEGF-C mRNA was measured by RT-PCR. ( B ) Expression level of VEGF-C mRNA was measured by means of real-time PCR for 48 hours. ( C ) The secretion of VEGF in serum of MCF-7 cell line after siRNA transfection. Abbreviations: VEGF, vascular endothelial growth factor; mRNA, messenger ribonucleic acid; RT-PCR, reverse-transcription polymerase chain reaction; siRNA, short interfering ribonucleic acid; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; <t>RNA,</t> ribonucleic acid; <t>RNAi,</t> ribonucleic acid interference.
    Ribosomal Ribonucleic Acid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Thermo Fisher sigenome smartpool sirna dharmacon thermo fisher per
    Effects of B7-H3 on the expression of VEGF in MCF-7 cells. Notes: ( A ) Expression level of VEGF-C mRNA was measured by RT-PCR. ( B ) Expression level of VEGF-C mRNA was measured by means of real-time PCR for 48 hours. ( C ) The secretion of VEGF in serum of MCF-7 cell line after siRNA transfection. Abbreviations: VEGF, vascular endothelial growth factor; mRNA, messenger ribonucleic acid; RT-PCR, reverse-transcription polymerase chain reaction; siRNA, short interfering ribonucleic acid; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; <t>RNA,</t> ribonucleic acid; <t>RNAi,</t> ribonucleic acid interference.
    Sigenome Smartpool Sirna Dharmacon Thermo Fisher Per, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    76
    Thermo Fisher vitro cp681301 efficiency test thermo fisher scientific
    CDK5 Directly Binds to and Phos-phorylates to Activate CREB1 in GSCs (A) <t>CP681301</t> treatment of mice xenografted explant culture tumors induced a clear down-regulation of CREB1 phosphorylation. (B) Western blot data using these tumor tissues also show downregulation of CREB1 phosphorylation. (C) We then investigated the mechanism by which CDK5 regulates CREB1. We found that Cdk5 can directly bind with CREB1, and CP681301 reduces the binding of CDK5 to CREB1. (D) An in vitro kinase assay showed that CDK5 can phosphorylate CREB1 at serine 133, and CP681301 can suppress this phosphorylation in a dose-dependent manner. (E) Diagram showing our proposed model where CDK5 directly phosphorylates and activates CREB1 without the requirement of cAMP/PKA in GSCs.
    Vitro Cp681301 Efficiency Test Thermo Fisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 76/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher kingfishertm thermo fisher automated extraction platform
    CDK5 Directly Binds to and Phos-phorylates to Activate CREB1 in GSCs (A) <t>CP681301</t> treatment of mice xenografted explant culture tumors induced a clear down-regulation of CREB1 phosphorylation. (B) Western blot data using these tumor tissues also show downregulation of CREB1 phosphorylation. (C) We then investigated the mechanism by which CDK5 regulates CREB1. We found that Cdk5 can directly bind with CREB1, and CP681301 reduces the binding of CDK5 to CREB1. (D) An in vitro kinase assay showed that CDK5 can phosphorylate CREB1 at serine 133, and CP681301 can suppress this phosphorylation in a dose-dependent manner. (E) Diagram showing our proposed model where CDK5 directly phosphorylates and activates CREB1 without the requirement of cAMP/PKA in GSCs.
    Kingfishertm Thermo Fisher Automated Extraction Platform, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher thermo fisher h3570
    CDK5 Directly Binds to and Phos-phorylates to Activate CREB1 in GSCs (A) <t>CP681301</t> treatment of mice xenografted explant culture tumors induced a clear down-regulation of CREB1 phosphorylation. (B) Western blot data using these tumor tissues also show downregulation of CREB1 phosphorylation. (C) We then investigated the mechanism by which CDK5 regulates CREB1. We found that Cdk5 can directly bind with CREB1, and CP681301 reduces the binding of CDK5 to CREB1. (D) An in vitro kinase assay showed that CDK5 can phosphorylate CREB1 at serine 133, and CP681301 can suppress this phosphorylation in a dose-dependent manner. (E) Diagram showing our proposed model where CDK5 directly phosphorylates and activates CREB1 without the requirement of cAMP/PKA in GSCs.
    Thermo Fisher H3570, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher thermo fisher s qubit 4 fluorometer
    CDK5 Directly Binds to and Phos-phorylates to Activate CREB1 in GSCs (A) <t>CP681301</t> treatment of mice xenografted explant culture tumors induced a clear down-regulation of CREB1 phosphorylation. (B) Western blot data using these tumor tissues also show downregulation of CREB1 phosphorylation. (C) We then investigated the mechanism by which CDK5 regulates CREB1. We found that Cdk5 can directly bind with CREB1, and CP681301 reduces the binding of CDK5 to CREB1. (D) An in vitro kinase assay showed that CDK5 can phosphorylate CREB1 at serine 133, and CP681301 can suppress this phosphorylation in a dose-dependent manner. (E) Diagram showing our proposed model where CDK5 directly phosphorylates and activates CREB1 without the requirement of cAMP/PKA in GSCs.
    Thermo Fisher S Qubit 4 Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher taq dna polymerase
    CDK5 Directly Binds to and Phos-phorylates to Activate CREB1 in GSCs (A) <t>CP681301</t> treatment of mice xenografted explant culture tumors induced a clear down-regulation of CREB1 phosphorylation. (B) Western blot data using these tumor tissues also show downregulation of CREB1 phosphorylation. (C) We then investigated the mechanism by which CDK5 regulates CREB1. We found that Cdk5 can directly bind with CREB1, and CP681301 reduces the binding of CDK5 to CREB1. (D) An in vitro kinase assay showed that CDK5 can phosphorylate CREB1 at serine 133, and CP681301 can suppress this phosphorylation in a dose-dependent manner. (E) Diagram showing our proposed model where CDK5 directly phosphorylates and activates CREB1 without the requirement of cAMP/PKA in GSCs.
    Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 31397 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Thermo Fisher protein kinase sirna library thermo fisher sigenome
    CDK5 Directly Binds to and Phos-phorylates to Activate CREB1 in GSCs (A) <t>CP681301</t> treatment of mice xenografted explant culture tumors induced a clear down-regulation of CREB1 phosphorylation. (B) Western blot data using these tumor tissues also show downregulation of CREB1 phosphorylation. (C) We then investigated the mechanism by which CDK5 regulates CREB1. We found that Cdk5 can directly bind with CREB1, and CP681301 reduces the binding of CDK5 to CREB1. (D) An in vitro kinase assay showed that CDK5 can phosphorylate CREB1 at serine 133, and CP681301 can suppress this phosphorylation in a dose-dependent manner. (E) Diagram showing our proposed model where CDK5 directly phosphorylates and activates CREB1 without the requirement of cAMP/PKA in GSCs.
    Protein Kinase Sirna Library Thermo Fisher Sigenome, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    CDK5 Directly Binds to and Phos-phorylates to Activate CREB1 in GSCs (A) <t>CP681301</t> treatment of mice xenografted explant culture tumors induced a clear down-regulation of CREB1 phosphorylation. (B) Western blot data using these tumor tissues also show downregulation of CREB1 phosphorylation. (C) We then investigated the mechanism by which CDK5 regulates CREB1. We found that Cdk5 can directly bind with CREB1, and CP681301 reduces the binding of CDK5 to CREB1. (D) An in vitro kinase assay showed that CDK5 can phosphorylate CREB1 at serine 133, and CP681301 can suppress this phosphorylation in a dose-dependent manner. (E) Diagram showing our proposed model where CDK5 directly phosphorylates and activates CREB1 without the requirement of cAMP/PKA in GSCs.
    Thermo Fisher Rrid Ab 10978021, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    CDK5 Directly Binds to and Phos-phorylates to Activate CREB1 in GSCs (A) <t>CP681301</t> treatment of mice xenografted explant culture tumors induced a clear down-regulation of CREB1 phosphorylation. (B) Western blot data using these tumor tissues also show downregulation of CREB1 phosphorylation. (C) We then investigated the mechanism by which CDK5 regulates CREB1. We found that Cdk5 can directly bind with CREB1, and CP681301 reduces the binding of CDK5 to CREB1. (D) An in vitro kinase assay showed that CDK5 can phosphorylate CREB1 at serine 133, and CP681301 can suppress this phosphorylation in a dose-dependent manner. (E) Diagram showing our proposed model where CDK5 directly phosphorylates and activates CREB1 without the requirement of cAMP/PKA in GSCs.
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    CDK5 Directly Binds to and Phos-phorylates to Activate CREB1 in GSCs (A) <t>CP681301</t> treatment of mice xenografted explant culture tumors induced a clear down-regulation of CREB1 phosphorylation. (B) Western blot data using these tumor tissues also show downregulation of CREB1 phosphorylation. (C) We then investigated the mechanism by which CDK5 regulates CREB1. We found that Cdk5 can directly bind with CREB1, and CP681301 reduces the binding of CDK5 to CREB1. (D) An in vitro kinase assay showed that CDK5 can phosphorylate CREB1 at serine 133, and CP681301 can suppress this phosphorylation in a dose-dependent manner. (E) Diagram showing our proposed model where CDK5 directly phosphorylates and activates CREB1 without the requirement of cAMP/PKA in GSCs.
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    Image Search Results


    Caspase-14 modulates trophoblast gene expression . The effect of caspase-14 siRNA on the expression of (A-C) hCG, (D-F) KLF4 and (G-I) cytokeratin-18 in DMSO-treated BeWo cells. * = P

    Journal: Reproductive Biology and Endocrinology : RB & E

    Article Title: Function of caspase-14 in trophoblast differentiation

    doi: 10.1186/1477-7827-7-98

    Figure Lengend Snippet: Caspase-14 modulates trophoblast gene expression . The effect of caspase-14 siRNA on the expression of (A-C) hCG, (D-F) KLF4 and (G-I) cytokeratin-18 in DMSO-treated BeWo cells. * = P

    Article Snippet: RNA interference (RNAi) RNAi was conducted by delivering 100 nM of caspase-14 short interfering ribonucleic acid (siRNA) (Invitrogen, Cat. No.1299003) into the cells using Lipofectamine2000 (Invitrogen, Cat. No. 11668) diluted in OptiMEM (Gibco, Cat. No. 31985) as the transfection agent, and incubated for 16 hours.

    Techniques: Expressing

    Caspase-14 modulates trophoblast gene expression during differentiation . The effect of caspase-14 siRNA on the expression of (A-C) hCG, (D-F) KLF4, and (G-I) cytokeratin-18 in Forskolin-treated BeWo cells. * = P

    Journal: Reproductive Biology and Endocrinology : RB & E

    Article Title: Function of caspase-14 in trophoblast differentiation

    doi: 10.1186/1477-7827-7-98

    Figure Lengend Snippet: Caspase-14 modulates trophoblast gene expression during differentiation . The effect of caspase-14 siRNA on the expression of (A-C) hCG, (D-F) KLF4, and (G-I) cytokeratin-18 in Forskolin-treated BeWo cells. * = P

    Article Snippet: RNA interference (RNAi) RNAi was conducted by delivering 100 nM of caspase-14 short interfering ribonucleic acid (siRNA) (Invitrogen, Cat. No.1299003) into the cells using Lipofectamine2000 (Invitrogen, Cat. No. 11668) diluted in OptiMEM (Gibco, Cat. No. 31985) as the transfection agent, and incubated for 16 hours.

    Techniques: Expressing

    Effects of B7-H3 on the expression of VEGF in MCF-7 cells. Notes: ( A ) Expression level of VEGF-C mRNA was measured by RT-PCR. ( B ) Expression level of VEGF-C mRNA was measured by means of real-time PCR for 48 hours. ( C ) The secretion of VEGF in serum of MCF-7 cell line after siRNA transfection. Abbreviations: VEGF, vascular endothelial growth factor; mRNA, messenger ribonucleic acid; RT-PCR, reverse-transcription polymerase chain reaction; siRNA, short interfering ribonucleic acid; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; RNA, ribonucleic acid; RNAi, ribonucleic acid interference.

    Journal: OncoTargets and therapy

    Article Title: B7-H3 expression in breast cancer and upregulation of VEGF through gene silence

    doi: 10.2147/OTT.S63424

    Figure Lengend Snippet: Effects of B7-H3 on the expression of VEGF in MCF-7 cells. Notes: ( A ) Expression level of VEGF-C mRNA was measured by RT-PCR. ( B ) Expression level of VEGF-C mRNA was measured by means of real-time PCR for 48 hours. ( C ) The secretion of VEGF in serum of MCF-7 cell line after siRNA transfection. Abbreviations: VEGF, vascular endothelial growth factor; mRNA, messenger ribonucleic acid; RT-PCR, reverse-transcription polymerase chain reaction; siRNA, short interfering ribonucleic acid; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; RNA, ribonucleic acid; RNAi, ribonucleic acid interference.

    Article Snippet: Three stealth ribonucleic acid (RNA) interference (RNAi) and two control RNAi were obtained from Invitrogen Life Technologies algorithms based against the Sequence Accession No AK075549 from GenBank.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Transfection

    Knockdown efficiency of B7-H3 by siRNA in MCF-7 cells. Notes: ( A ) B7-H3 mRNA expression was measured by RT-PCR. Lane 1: Cells were treated with siRNA of control. Lane 2: Cells were treated with siRNA of B7-H3 for 48 hours. Lane 3: Marker DL2000. ( B ) Expression level of B7-H3 mRNA was measured by means of real-time PCR 48 hours after siRNA transfection. Values are mean ± standard deviation of triplicate samples. Abbreviations: siRNA, short interfering ribonucleic acid; mRNA, messenger ribonucleic acid; RT-PCR, reverse-transcription polymerase chain reaction; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; bp, base pair; PCR, polymerase chain reaction; RNA, ribonucleic acid; RNAi, ribonucleic acid interference.

    Journal: OncoTargets and therapy

    Article Title: B7-H3 expression in breast cancer and upregulation of VEGF through gene silence

    doi: 10.2147/OTT.S63424

    Figure Lengend Snippet: Knockdown efficiency of B7-H3 by siRNA in MCF-7 cells. Notes: ( A ) B7-H3 mRNA expression was measured by RT-PCR. Lane 1: Cells were treated with siRNA of control. Lane 2: Cells were treated with siRNA of B7-H3 for 48 hours. Lane 3: Marker DL2000. ( B ) Expression level of B7-H3 mRNA was measured by means of real-time PCR 48 hours after siRNA transfection. Values are mean ± standard deviation of triplicate samples. Abbreviations: siRNA, short interfering ribonucleic acid; mRNA, messenger ribonucleic acid; RT-PCR, reverse-transcription polymerase chain reaction; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; bp, base pair; PCR, polymerase chain reaction; RNA, ribonucleic acid; RNAi, ribonucleic acid interference.

    Article Snippet: Three stealth ribonucleic acid (RNA) interference (RNAi) and two control RNAi were obtained from Invitrogen Life Technologies algorithms based against the Sequence Accession No AK075549 from GenBank.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Marker, Real-time Polymerase Chain Reaction, Transfection, Standard Deviation, Polymerase Chain Reaction

    CDK5 Directly Binds to and Phos-phorylates to Activate CREB1 in GSCs (A) CP681301 treatment of mice xenografted explant culture tumors induced a clear down-regulation of CREB1 phosphorylation. (B) Western blot data using these tumor tissues also show downregulation of CREB1 phosphorylation. (C) We then investigated the mechanism by which CDK5 regulates CREB1. We found that Cdk5 can directly bind with CREB1, and CP681301 reduces the binding of CDK5 to CREB1. (D) An in vitro kinase assay showed that CDK5 can phosphorylate CREB1 at serine 133, and CP681301 can suppress this phosphorylation in a dose-dependent manner. (E) Diagram showing our proposed model where CDK5 directly phosphorylates and activates CREB1 without the requirement of cAMP/PKA in GSCs.

    Journal: Cell reports

    Article Title: CDK5 Inhibition Resolves PKA/cAMP-Independent Activation of CREB1 Signaling in Glioma Stem Cells

    doi: 10.1016/j.celrep.2018.04.016

    Figure Lengend Snippet: CDK5 Directly Binds to and Phos-phorylates to Activate CREB1 in GSCs (A) CP681301 treatment of mice xenografted explant culture tumors induced a clear down-regulation of CREB1 phosphorylation. (B) Western blot data using these tumor tissues also show downregulation of CREB1 phosphorylation. (C) We then investigated the mechanism by which CDK5 regulates CREB1. We found that Cdk5 can directly bind with CREB1, and CP681301 reduces the binding of CDK5 to CREB1. (D) An in vitro kinase assay showed that CDK5 can phosphorylate CREB1 at serine 133, and CP681301 can suppress this phosphorylation in a dose-dependent manner. (E) Diagram showing our proposed model where CDK5 directly phosphorylates and activates CREB1 without the requirement of cAMP/PKA in GSCs.

    Article Snippet: In Vitro CP681301 Efficiency Test Thermo Fisher Scientific tested the inhibitory efficiency of CP681301 on active CDK5/P25 and CDK5/P35 in vitro .

    Techniques: Mouse Assay, Western Blot, Binding Assay, In Vitro, Kinase Assay

    CDK5 Regulates Self-Renewal Properties in GSCs (A) To determine if CDK5 regulates self-renewal of GSCs, first we tested the potency of a highly specific CDK5 inhibitor called CP681301 from Pfizer. Using active CDK5-p35 and CDK5-p25 as enzymes, we established a dose-response curve for CP681301 in order to find the doses that successfully inhibit kinase catalytic activity of both protein complexes in vitro . 1 μM CP681301 was the optimal concentration that suppressed 100% of CDK5-p35 and CDK5-p25 catalytic activity. (B) CP681301 (1 μM) for 96 hr is significantly less toxic to NHNPs (control) than GSCs. *,

    Journal: Cell reports

    Article Title: CDK5 Inhibition Resolves PKA/cAMP-Independent Activation of CREB1 Signaling in Glioma Stem Cells

    doi: 10.1016/j.celrep.2018.04.016

    Figure Lengend Snippet: CDK5 Regulates Self-Renewal Properties in GSCs (A) To determine if CDK5 regulates self-renewal of GSCs, first we tested the potency of a highly specific CDK5 inhibitor called CP681301 from Pfizer. Using active CDK5-p35 and CDK5-p25 as enzymes, we established a dose-response curve for CP681301 in order to find the doses that successfully inhibit kinase catalytic activity of both protein complexes in vitro . 1 μM CP681301 was the optimal concentration that suppressed 100% of CDK5-p35 and CDK5-p25 catalytic activity. (B) CP681301 (1 μM) for 96 hr is significantly less toxic to NHNPs (control) than GSCs. *,

    Article Snippet: In Vitro CP681301 Efficiency Test Thermo Fisher Scientific tested the inhibitory efficiency of CP681301 on active CDK5/P25 and CDK5/P35 in vitro .

    Techniques: Activity Assay, In Vitro, Concentration Assay

    CDK5 Inhibitor Suppresses Self-Renewal and Proliferation of GBM Xenograft Tumors in Explant Cultures (A) Diagram shows the mice xenograft tumor implantation and explant culture flow chart. Two months after implantation, GBM121 and GBM39 both produce considerably large tumors on the right side of the brains. (B) Tumor sections (300 μm) were cultured on Petri dishes for 48 hr with 0, 10, and 50 μM CP681301. Immunohistochemical analysis shows significant reduction of active CDK5 and Ki67 staining in the tumor in cultures sections treated with CP681301 relative to control. (C) Western blots performed on protein lysates created from xenografted tumors show downregulation of stem cell markers and confirm suppression of CDK5 phosphorylation through ABL and cell proliferation (Ki67). (D) Investigating putative downstream targets of CDK5, we found that phosphorylated (p)-RB (inactive RB), p-MYC (active MYC), and p-CREB1 are all suppressed by CP681301 treatment. (E) Among these, the expression of CREB1 and CDK5 are strongly correlated in TCGA U133 GBM data. (F) Additionally, TCGA RNA-seq RSEM data showed that CREB1 is positively correlated with iTPC transcription factors SOX2 , OLIG2 , SALL2 , and POU3F2 . (G) Analysis of TCGA RNA-seq RSEM data also showed that CREB1 is strongly correlated with all four of these transcription factors, whereas MYC is only correlated with two of these. (H) Immunocytological data also supports that CDK5 inhibition reduces CREB1 phosphorylation. *p

    Journal: Cell reports

    Article Title: CDK5 Inhibition Resolves PKA/cAMP-Independent Activation of CREB1 Signaling in Glioma Stem Cells

    doi: 10.1016/j.celrep.2018.04.016

    Figure Lengend Snippet: CDK5 Inhibitor Suppresses Self-Renewal and Proliferation of GBM Xenograft Tumors in Explant Cultures (A) Diagram shows the mice xenograft tumor implantation and explant culture flow chart. Two months after implantation, GBM121 and GBM39 both produce considerably large tumors on the right side of the brains. (B) Tumor sections (300 μm) were cultured on Petri dishes for 48 hr with 0, 10, and 50 μM CP681301. Immunohistochemical analysis shows significant reduction of active CDK5 and Ki67 staining in the tumor in cultures sections treated with CP681301 relative to control. (C) Western blots performed on protein lysates created from xenografted tumors show downregulation of stem cell markers and confirm suppression of CDK5 phosphorylation through ABL and cell proliferation (Ki67). (D) Investigating putative downstream targets of CDK5, we found that phosphorylated (p)-RB (inactive RB), p-MYC (active MYC), and p-CREB1 are all suppressed by CP681301 treatment. (E) Among these, the expression of CREB1 and CDK5 are strongly correlated in TCGA U133 GBM data. (F) Additionally, TCGA RNA-seq RSEM data showed that CREB1 is positively correlated with iTPC transcription factors SOX2 , OLIG2 , SALL2 , and POU3F2 . (G) Analysis of TCGA RNA-seq RSEM data also showed that CREB1 is strongly correlated with all four of these transcription factors, whereas MYC is only correlated with two of these. (H) Immunocytological data also supports that CDK5 inhibition reduces CREB1 phosphorylation. *p

    Article Snippet: In Vitro CP681301 Efficiency Test Thermo Fisher Scientific tested the inhibitory efficiency of CP681301 on active CDK5/P25 and CDK5/P35 in vitro .

    Techniques: Mouse Assay, Tumor Implantation, Flow Cytometry, Cell Culture, Immunohistochemistry, Staining, Western Blot, Expressing, RNA Sequencing Assay, Inhibition

    CDK5 Regulates Self-Renewal and Asymmetric Cell Division in Drosophila Neuroblasts (A) Drosophila eye screen. (i) Drosophila normal eye. (ii) brat -RNAi using eyeless-GAL4 drives overgrowth of the eye. Using this phenotype, we performed a suppressor screen for kinases and found that reducing dcdk5 (v) and its activating partner, dp35 (iv), can reverse the overgrowth successfully. Suppression of two other kinases, dS6k (iii) and dEip63E (vi), did not revert the overgrowth completely. Graphs of the suppressor screen experiments show statistical significance. (B) Diagrams represent Drosophila P35 and Cdk5 are identical to human P35 ( CDK5R1 ) and human CDK5 by 66% and 79%, respectively. (C) brat -RNAi adult brain tumor shows high Mira expression (green) (i), indicating deregulated asymmetric division that generated these tumor stem cells. These cells also express high levels of active dCdk5 (white) (ii) compared with normal cells. RNAi suppression of dP35 and dCdk5 in brat -RNAi tumors reduced cell proliferation (PH3 staining, white) (iii, iv, and v) and the self-renewal marker (Mira, green) (vi, vii, and viii). Statistics are in Figure S1 . (D) We tested the potency of Cdk5 as an asymmetric cell division regulator by knocking down dCdk5 and by overexpressing human CDK5 specifically in neuroblasts. During asymmetric division, larval neuroblast mother cells (Mira positive, green) generate progenitors that are Mira negative and eventually differentiate (i, ii, and iii). Knock down of dCdk5 using RNAi specifically in neuroblasts reduced Mira expression without any visible change in daughter cells (red) (iv, v, and vi). However, ectopic expression of hCDK5 in neuroblasts disrupted the asymmetric division, generating more Mira-positive cells (vii, viii, and ix). Statistics are in Figure S1 . (E) CP681301, a specific CDK5 inhibitor, was fed to adult flies with brat -RNAi tumors. After 10 days of feeding, adult tumors lost expression of stem cell markers (Mira, green) (i and ii) and active phosphorylated dCdk5 expression (white) (iii and iv). (F) Suppressing dCdk5 activity with CP681301 increased the median survival of the brat -RNAi tumor-carrying flies by 2.85-fold. p

    Journal: Cell reports

    Article Title: CDK5 Inhibition Resolves PKA/cAMP-Independent Activation of CREB1 Signaling in Glioma Stem Cells

    doi: 10.1016/j.celrep.2018.04.016

    Figure Lengend Snippet: CDK5 Regulates Self-Renewal and Asymmetric Cell Division in Drosophila Neuroblasts (A) Drosophila eye screen. (i) Drosophila normal eye. (ii) brat -RNAi using eyeless-GAL4 drives overgrowth of the eye. Using this phenotype, we performed a suppressor screen for kinases and found that reducing dcdk5 (v) and its activating partner, dp35 (iv), can reverse the overgrowth successfully. Suppression of two other kinases, dS6k (iii) and dEip63E (vi), did not revert the overgrowth completely. Graphs of the suppressor screen experiments show statistical significance. (B) Diagrams represent Drosophila P35 and Cdk5 are identical to human P35 ( CDK5R1 ) and human CDK5 by 66% and 79%, respectively. (C) brat -RNAi adult brain tumor shows high Mira expression (green) (i), indicating deregulated asymmetric division that generated these tumor stem cells. These cells also express high levels of active dCdk5 (white) (ii) compared with normal cells. RNAi suppression of dP35 and dCdk5 in brat -RNAi tumors reduced cell proliferation (PH3 staining, white) (iii, iv, and v) and the self-renewal marker (Mira, green) (vi, vii, and viii). Statistics are in Figure S1 . (D) We tested the potency of Cdk5 as an asymmetric cell division regulator by knocking down dCdk5 and by overexpressing human CDK5 specifically in neuroblasts. During asymmetric division, larval neuroblast mother cells (Mira positive, green) generate progenitors that are Mira negative and eventually differentiate (i, ii, and iii). Knock down of dCdk5 using RNAi specifically in neuroblasts reduced Mira expression without any visible change in daughter cells (red) (iv, v, and vi). However, ectopic expression of hCDK5 in neuroblasts disrupted the asymmetric division, generating more Mira-positive cells (vii, viii, and ix). Statistics are in Figure S1 . (E) CP681301, a specific CDK5 inhibitor, was fed to adult flies with brat -RNAi tumors. After 10 days of feeding, adult tumors lost expression of stem cell markers (Mira, green) (i and ii) and active phosphorylated dCdk5 expression (white) (iii and iv). (F) Suppressing dCdk5 activity with CP681301 increased the median survival of the brat -RNAi tumor-carrying flies by 2.85-fold. p

    Article Snippet: In Vitro CP681301 Efficiency Test Thermo Fisher Scientific tested the inhibitory efficiency of CP681301 on active CDK5/P25 and CDK5/P35 in vitro .

    Techniques: Expressing, Generated, Staining, Marker, Activity Assay