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  • 95
    Millipore rna
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    Agilent technologies ribonucleic acid analysis
    Ribonucleic Acid Analysis, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies epstein barr virus ribonucleic acid
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    Agilent technologies messenger ribonucleic acid mrna expression
    Messenger Ribonucleic Acid Mrna Expression, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies rna agilent technologies
    Effect of Filamin A and actin cytoskeleton on RNase L activity. M2 stable cell lines expressing Myc-Filamin A and/or Flag-RNase L WT or Flag-RNase L R667A were analyzed for expression of RNase L and Filamin A on immunoblots (A) and transfected with 10 µM 2-5A (B), and RNase L-mediated cleavage of <t>rRNA</t> (arrows) was analyzed on <t>RNA</t> chips using an Agilent 2100 Bioanalyzer. (C) HT1080 cells were pretreated with Cytochalasin D (Cyto D; 5 µM), Latrunculin A (Lat A; 0.5 µM), or vehicle (DMSO) for 1 h followed by transfection with 10 µM 2-5A or 2 µg/ml of poly(I·C). Characteristic RNase L-generated rRNA cleavage products (arrows) were detected as described for panel B. Representative images from three independent experiments are shown.
    Rna Agilent Technologies, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 83/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies agilent technologies spike in rna
    Effect of Filamin A and actin cytoskeleton on RNase L activity. M2 stable cell lines expressing Myc-Filamin A and/or Flag-RNase L WT or Flag-RNase L R667A were analyzed for expression of RNase L and Filamin A on immunoblots (A) and transfected with 10 µM 2-5A (B), and RNase L-mediated cleavage of <t>rRNA</t> (arrows) was analyzed on <t>RNA</t> chips using an Agilent 2100 Bioanalyzer. (C) HT1080 cells were pretreated with Cytochalasin D (Cyto D; 5 µM), Latrunculin A (Lat A; 0.5 µM), or vehicle (DMSO) for 1 h followed by transfection with 10 µM 2-5A or 2 µg/ml of poly(I·C). Characteristic RNase L-generated rRNA cleavage products (arrows) were detected as described for panel B. Representative images from three independent experiments are shown.
    Agilent Technologies Spike In Rna, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies agilent technologies rna nanochip
    Experimental workflow to assess efficiency of NuGen probe generation technologies using low amounts of input <t>RNA.</t> HUVEC total RNA was titrated to cover a range of input RNA from 50 ng–10 pg. 50 ng (n = 1), 500 pg (n = 2) and 250 pg (n = 2) of total RNA was used as input for the WT-Ovation FFPE system V2 while 500 pg (n = 2), 250 pg (n = 2), 100 pg (n = 2), 50 pg (n = 2) and 10 pg (n = 2) were used as input for the WT-Ovation One-Direct system (NuGen Technologies, Inc). All cDNA reactions were purified via Zymo Research Clean and Concentrator™-25 or Qiagen RNeasy MinElute Cleanup kits (WT-Ovation FFPE V2 and WT-Ovation One-Direct systems respectively) as recommended. All purified cDNA probes were assessed for quantity and quality using the Agilent 2100 Bioanalyzer and the Nanodrop-8000 RNA <t>Nano</t> chips. FL-Ovation™ cDNA Biotin Module V2 (NuGEN) was used for fragmentation and biotin labelling of 5 µg of cDNA and used for subsequent hybridisation to Affymetrix HGU133 Plus 2.0 microarrays.
    Agilent Technologies Rna Nanochip, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 77/100, based on 113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies agilent technologies bioanalyzer rna
    Experimental workflow to assess efficiency of NuGen probe generation technologies using low amounts of input <t>RNA.</t> HUVEC total RNA was titrated to cover a range of input RNA from 50 ng–10 pg. 50 ng (n = 1), 500 pg (n = 2) and 250 pg (n = 2) of total RNA was used as input for the WT-Ovation FFPE system V2 while 500 pg (n = 2), 250 pg (n = 2), 100 pg (n = 2), 50 pg (n = 2) and 10 pg (n = 2) were used as input for the WT-Ovation One-Direct system (NuGen Technologies, Inc). All cDNA reactions were purified via Zymo Research Clean and Concentrator™-25 or Qiagen RNeasy MinElute Cleanup kits (WT-Ovation FFPE V2 and WT-Ovation One-Direct systems respectively) as recommended. All purified cDNA probes were assessed for quantity and quality using the Agilent 2100 Bioanalyzer and the Nanodrop-8000 RNA <t>Nano</t> chips. FL-Ovation™ cDNA Biotin Module V2 (NuGEN) was used for fragmentation and biotin labelling of 5 µg of cDNA and used for subsequent hybridisation to Affymetrix HGU133 Plus 2.0 microarrays.
    Agilent Technologies Bioanalyzer Rna, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 77/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Experimental workflow to assess efficiency of NuGen probe generation technologies using low amounts of input <t>RNA.</t> HUVEC total RNA was titrated to cover a range of input RNA from 50 ng–10 pg. 50 ng (n = 1), 500 pg (n = 2) and 250 pg (n = 2) of total RNA was used as input for the WT-Ovation FFPE system V2 while 500 pg (n = 2), 250 pg (n = 2), 100 pg (n = 2), 50 pg (n = 2) and 10 pg (n = 2) were used as input for the WT-Ovation One-Direct system (NuGen Technologies, Inc). All cDNA reactions were purified via Zymo Research Clean and Concentrator™-25 or Qiagen RNeasy MinElute Cleanup kits (WT-Ovation FFPE V2 and WT-Ovation One-Direct systems respectively) as recommended. All purified cDNA probes were assessed for quantity and quality using the Agilent 2100 Bioanalyzer and the Nanodrop-8000 RNA <t>Nano</t> chips. FL-Ovation™ cDNA Biotin Module V2 (NuGEN) was used for fragmentation and biotin labelling of 5 µg of cDNA and used for subsequent hybridisation to Affymetrix HGU133 Plus 2.0 microarrays.
    Bioanalyzer Agilent Rna 6000 Pico Kit Agilent Technologies, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies analyser agilent rna 6000 nano kit agilent technologies germany
    Experimental workflow to assess efficiency of NuGen probe generation technologies using low amounts of input <t>RNA.</t> HUVEC total RNA was titrated to cover a range of input RNA from 50 ng–10 pg. 50 ng (n = 1), 500 pg (n = 2) and 250 pg (n = 2) of total RNA was used as input for the WT-Ovation FFPE system V2 while 500 pg (n = 2), 250 pg (n = 2), 100 pg (n = 2), 50 pg (n = 2) and 10 pg (n = 2) were used as input for the WT-Ovation One-Direct system (NuGen Technologies, Inc). All cDNA reactions were purified via Zymo Research Clean and Concentrator™-25 or Qiagen RNeasy MinElute Cleanup kits (WT-Ovation FFPE V2 and WT-Ovation One-Direct systems respectively) as recommended. All purified cDNA probes were assessed for quantity and quality using the Agilent 2100 Bioanalyzer and the Nanodrop-8000 RNA <t>Nano</t> chips. FL-Ovation™ cDNA Biotin Module V2 (NuGEN) was used for fragmentation and biotin labelling of 5 µg of cDNA and used for subsequent hybridisation to Affymetrix HGU133 Plus 2.0 microarrays.
    Analyser Agilent Rna 6000 Nano Kit Agilent Technologies Germany, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies agilent technologies 2100 bioanalyzer
    Experimental workflow to assess efficiency of NuGen probe generation technologies using low amounts of input <t>RNA.</t> HUVEC total RNA was titrated to cover a range of input RNA from 50 ng–10 pg. 50 ng (n = 1), 500 pg (n = 2) and 250 pg (n = 2) of total RNA was used as input for the WT-Ovation FFPE system V2 while 500 pg (n = 2), 250 pg (n = 2), 100 pg (n = 2), 50 pg (n = 2) and 10 pg (n = 2) were used as input for the WT-Ovation One-Direct system (NuGen Technologies, Inc). All cDNA reactions were purified via Zymo Research Clean and Concentrator™-25 or Qiagen RNeasy MinElute Cleanup kits (WT-Ovation FFPE V2 and WT-Ovation One-Direct systems respectively) as recommended. All purified cDNA probes were assessed for quantity and quality using the Agilent 2100 Bioanalyzer and the Nanodrop-8000 RNA <t>Nano</t> chips. FL-Ovation™ cDNA Biotin Module V2 (NuGEN) was used for fragmentation and biotin labelling of 5 µg of cDNA and used for subsequent hybridisation to Affymetrix HGU133 Plus 2.0 microarrays.
    Agilent Technologies 2100 Bioanalyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 3806 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Experimental workflow to assess efficiency of NuGen probe generation technologies using low amounts of input <t>RNA.</t> HUVEC total RNA was titrated to cover a range of input RNA from 50 ng–10 pg. 50 ng (n = 1), 500 pg (n = 2) and 250 pg (n = 2) of total RNA was used as input for the WT-Ovation FFPE system V2 while 500 pg (n = 2), 250 pg (n = 2), 100 pg (n = 2), 50 pg (n = 2) and 10 pg (n = 2) were used as input for the WT-Ovation One-Direct system (NuGen Technologies, Inc). All cDNA reactions were purified via Zymo Research Clean and Concentrator™-25 or Qiagen RNeasy MinElute Cleanup kits (WT-Ovation FFPE V2 and WT-Ovation One-Direct systems respectively) as recommended. All purified cDNA probes were assessed for quantity and quality using the Agilent 2100 Bioanalyzer and the Nanodrop-8000 RNA <t>Nano</t> chips. FL-Ovation™ cDNA Biotin Module V2 (NuGEN) was used for fragmentation and biotin labelling of 5 µg of cDNA and used for subsequent hybridisation to Affymetrix HGU133 Plus 2.0 microarrays.
    Agilent Bioanalyzer Agilent Technologies, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 80/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies 2100 bioanalyzer agilent rna 6000 nano kit agilent technologies
    Experimental workflow to assess efficiency of NuGen probe generation technologies using low amounts of input <t>RNA.</t> HUVEC total RNA was titrated to cover a range of input RNA from 50 ng–10 pg. 50 ng (n = 1), 500 pg (n = 2) and 250 pg (n = 2) of total RNA was used as input for the WT-Ovation FFPE system V2 while 500 pg (n = 2), 250 pg (n = 2), 100 pg (n = 2), 50 pg (n = 2) and 10 pg (n = 2) were used as input for the WT-Ovation One-Direct system (NuGen Technologies, Inc). All cDNA reactions were purified via Zymo Research Clean and Concentrator™-25 or Qiagen RNeasy MinElute Cleanup kits (WT-Ovation FFPE V2 and WT-Ovation One-Direct systems respectively) as recommended. All purified cDNA probes were assessed for quantity and quality using the Agilent 2100 Bioanalyzer and the Nanodrop-8000 RNA <t>Nano</t> chips. FL-Ovation™ cDNA Biotin Module V2 (NuGEN) was used for fragmentation and biotin labelling of 5 µg of cDNA and used for subsequent hybridisation to Affymetrix HGU133 Plus 2.0 microarrays.
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    Experimental workflow to assess efficiency of NuGen probe generation technologies using low amounts of input <t>RNA.</t> HUVEC total RNA was titrated to cover a range of input RNA from 50 ng–10 pg. 50 ng (n = 1), 500 pg (n = 2) and 250 pg (n = 2) of total RNA was used as input for the WT-Ovation FFPE system V2 while 500 pg (n = 2), 250 pg (n = 2), 100 pg (n = 2), 50 pg (n = 2) and 10 pg (n = 2) were used as input for the WT-Ovation One-Direct system (NuGen Technologies, Inc). All cDNA reactions were purified via Zymo Research Clean and Concentrator™-25 or Qiagen RNeasy MinElute Cleanup kits (WT-Ovation FFPE V2 and WT-Ovation One-Direct systems respectively) as recommended. All purified cDNA probes were assessed for quantity and quality using the Agilent 2100 Bioanalyzer and the Nanodrop-8000 RNA <t>Nano</t> chips. FL-Ovation™ cDNA Biotin Module V2 (NuGEN) was used for fragmentation and biotin labelling of 5 µg of cDNA and used for subsequent hybridisation to Affymetrix HGU133 Plus 2.0 microarrays.
    Bioanalyzer Agilent Rna 6000 Nano Kit Agilent Technologies Germany, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies disposable rna chips agilent rna 6000 nano labchip kit agilent technologies
    Experimental workflow to assess efficiency of NuGen probe generation technologies using low amounts of input <t>RNA.</t> HUVEC total RNA was titrated to cover a range of input RNA from 50 ng–10 pg. 50 ng (n = 1), 500 pg (n = 2) and 250 pg (n = 2) of total RNA was used as input for the WT-Ovation FFPE system V2 while 500 pg (n = 2), 250 pg (n = 2), 100 pg (n = 2), 50 pg (n = 2) and 10 pg (n = 2) were used as input for the WT-Ovation One-Direct system (NuGen Technologies, Inc). All cDNA reactions were purified via Zymo Research Clean and Concentrator™-25 or Qiagen RNeasy MinElute Cleanup kits (WT-Ovation FFPE V2 and WT-Ovation One-Direct systems respectively) as recommended. All purified cDNA probes were assessed for quantity and quality using the Agilent 2100 Bioanalyzer and the Nanodrop-8000 RNA <t>Nano</t> chips. FL-Ovation™ cDNA Biotin Module V2 (NuGEN) was used for fragmentation and biotin labelling of 5 µg of cDNA and used for subsequent hybridisation to Affymetrix HGU133 Plus 2.0 microarrays.
    Disposable Rna Chips Agilent Rna 6000 Nano Labchip Kit Agilent Technologies, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 80/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies 2100 bioanalyser rna 6000 pico kit agilent technologies
    Experimental workflow to assess efficiency of NuGen probe generation technologies using low amounts of input <t>RNA.</t> HUVEC total RNA was titrated to cover a range of input RNA from 50 ng–10 pg. 50 ng (n = 1), 500 pg (n = 2) and 250 pg (n = 2) of total RNA was used as input for the WT-Ovation FFPE system V2 while 500 pg (n = 2), 250 pg (n = 2), 100 pg (n = 2), 50 pg (n = 2) and 10 pg (n = 2) were used as input for the WT-Ovation One-Direct system (NuGen Technologies, Inc). All cDNA reactions were purified via Zymo Research Clean and Concentrator™-25 or Qiagen RNeasy MinElute Cleanup kits (WT-Ovation FFPE V2 and WT-Ovation One-Direct systems respectively) as recommended. All purified cDNA probes were assessed for quantity and quality using the Agilent 2100 Bioanalyzer and the Nanodrop-8000 RNA <t>Nano</t> chips. FL-Ovation™ cDNA Biotin Module V2 (NuGEN) was used for fragmentation and biotin labelling of 5 µg of cDNA and used for subsequent hybridisation to Affymetrix HGU133 Plus 2.0 microarrays.
    2100 Bioanalyser Rna 6000 Pico Kit Agilent Technologies, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies gto neuronal subsets absolutely rna nanoprep kit agilent technologies
    Experimental workflow to assess efficiency of NuGen probe generation technologies using low amounts of input <t>RNA.</t> HUVEC total RNA was titrated to cover a range of input RNA from 50 ng–10 pg. 50 ng (n = 1), 500 pg (n = 2) and 250 pg (n = 2) of total RNA was used as input for the WT-Ovation FFPE system V2 while 500 pg (n = 2), 250 pg (n = 2), 100 pg (n = 2), 50 pg (n = 2) and 10 pg (n = 2) were used as input for the WT-Ovation One-Direct system (NuGen Technologies, Inc). All cDNA reactions were purified via Zymo Research Clean and Concentrator™-25 or Qiagen RNeasy MinElute Cleanup kits (WT-Ovation FFPE V2 and WT-Ovation One-Direct systems respectively) as recommended. All purified cDNA probes were assessed for quantity and quality using the Agilent 2100 Bioanalyzer and the Nanodrop-8000 RNA <t>Nano</t> chips. FL-Ovation™ cDNA Biotin Module V2 (NuGEN) was used for fragmentation and biotin labelling of 5 µg of cDNA and used for subsequent hybridisation to Affymetrix HGU133 Plus 2.0 microarrays.
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    Experimental workflow to assess efficiency of NuGen probe generation technologies using low amounts of input <t>RNA.</t> HUVEC total RNA was titrated to cover a range of input RNA from 50 ng–10 pg. 50 ng (n = 1), 500 pg (n = 2) and 250 pg (n = 2) of total RNA was used as input for the WT-Ovation FFPE system V2 while 500 pg (n = 2), 250 pg (n = 2), 100 pg (n = 2), 50 pg (n = 2) and 10 pg (n = 2) were used as input for the WT-Ovation One-Direct system (NuGen Technologies, Inc). All cDNA reactions were purified via Zymo Research Clean and Concentrator™-25 or Qiagen RNeasy MinElute Cleanup kits (WT-Ovation FFPE V2 and WT-Ovation One-Direct systems respectively) as recommended. All purified cDNA probes were assessed for quantity and quality using the Agilent 2100 Bioanalyzer and the Nanodrop-8000 RNA <t>Nano</t> chips. FL-Ovation™ cDNA Biotin Module V2 (NuGEN) was used for fragmentation and biotin labelling of 5 µg of cDNA and used for subsequent hybridisation to Affymetrix HGU133 Plus 2.0 microarrays.
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    Experimental workflow to assess efficiency of NuGen probe generation technologies using low amounts of input <t>RNA.</t> HUVEC total RNA was titrated to cover a range of input RNA from 50 ng–10 pg. 50 ng (n = 1), 500 pg (n = 2) and 250 pg (n = 2) of total RNA was used as input for the WT-Ovation FFPE system V2 while 500 pg (n = 2), 250 pg (n = 2), 100 pg (n = 2), 50 pg (n = 2) and 10 pg (n = 2) were used as input for the WT-Ovation One-Direct system (NuGen Technologies, Inc). All cDNA reactions were purified via Zymo Research Clean and Concentrator™-25 or Qiagen RNeasy MinElute Cleanup kits (WT-Ovation FFPE V2 and WT-Ovation One-Direct systems respectively) as recommended. All purified cDNA probes were assessed for quantity and quality using the Agilent 2100 Bioanalyzer and the Nanodrop-8000 RNA <t>Nano</t> chips. FL-Ovation™ cDNA Biotin Module V2 (NuGEN) was used for fragmentation and biotin labelling of 5 µg of cDNA and used for subsequent hybridisation to Affymetrix HGU133 Plus 2.0 microarrays.
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    Experimental workflow to assess efficiency of NuGen probe generation technologies using low amounts of input <t>RNA.</t> HUVEC total RNA was titrated to cover a range of input RNA from 50 ng–10 pg. 50 ng (n = 1), 500 pg (n = 2) and 250 pg (n = 2) of total RNA was used as input for the WT-Ovation FFPE system V2 while 500 pg (n = 2), 250 pg (n = 2), 100 pg (n = 2), 50 pg (n = 2) and 10 pg (n = 2) were used as input for the WT-Ovation One-Direct system (NuGen Technologies, Inc). All cDNA reactions were purified via Zymo Research Clean and Concentrator™-25 or Qiagen RNeasy MinElute Cleanup kits (WT-Ovation FFPE V2 and WT-Ovation One-Direct systems respectively) as recommended. All purified cDNA probes were assessed for quantity and quality using the Agilent 2100 Bioanalyzer and the Nanodrop-8000 RNA <t>Nano</t> chips. FL-Ovation™ cDNA Biotin Module V2 (NuGEN) was used for fragmentation and biotin labelling of 5 µg of cDNA and used for subsequent hybridisation to Affymetrix HGU133 Plus 2.0 microarrays.
    Agilent Technologies Bioanalyzer Labchip, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Experimental workflow to assess efficiency of NuGen probe generation technologies using low amounts of input <t>RNA.</t> HUVEC total RNA was titrated to cover a range of input RNA from 50 ng–10 pg. 50 ng (n = 1), 500 pg (n = 2) and 250 pg (n = 2) of total RNA was used as input for the WT-Ovation FFPE system V2 while 500 pg (n = 2), 250 pg (n = 2), 100 pg (n = 2), 50 pg (n = 2) and 10 pg (n = 2) were used as input for the WT-Ovation One-Direct system (NuGen Technologies, Inc). All cDNA reactions were purified via Zymo Research Clean and Concentrator™-25 or Qiagen RNeasy MinElute Cleanup kits (WT-Ovation FFPE V2 and WT-Ovation One-Direct systems respectively) as recommended. All purified cDNA probes were assessed for quantity and quality using the Agilent 2100 Bioanalyzer and the Nanodrop-8000 RNA <t>Nano</t> chips. FL-Ovation™ cDNA Biotin Module V2 (NuGEN) was used for fragmentation and biotin labelling of 5 µg of cDNA and used for subsequent hybridisation to Affymetrix HGU133 Plus 2.0 microarrays.
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    Experimental workflow to assess efficiency of NuGen probe generation technologies using low amounts of input <t>RNA.</t> HUVEC total RNA was titrated to cover a range of input RNA from 50 ng–10 pg. 50 ng (n = 1), 500 pg (n = 2) and 250 pg (n = 2) of total RNA was used as input for the WT-Ovation FFPE system V2 while 500 pg (n = 2), 250 pg (n = 2), 100 pg (n = 2), 50 pg (n = 2) and 10 pg (n = 2) were used as input for the WT-Ovation One-Direct system (NuGen Technologies, Inc). All cDNA reactions were purified via Zymo Research Clean and Concentrator™-25 or Qiagen RNeasy MinElute Cleanup kits (WT-Ovation FFPE V2 and WT-Ovation One-Direct systems respectively) as recommended. All purified cDNA probes were assessed for quantity and quality using the Agilent 2100 Bioanalyzer and the Nanodrop-8000 RNA <t>Nano</t> chips. FL-Ovation™ cDNA Biotin Module V2 (NuGEN) was used for fragmentation and biotin labelling of 5 µg of cDNA and used for subsequent hybridisation to Affymetrix HGU133 Plus 2.0 microarrays.
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    Experimental workflow to assess efficiency of NuGen probe generation technologies using low amounts of input <t>RNA.</t> HUVEC total RNA was titrated to cover a range of input RNA from 50 ng–10 pg. 50 ng (n = 1), 500 pg (n = 2) and 250 pg (n = 2) of total RNA was used as input for the WT-Ovation FFPE system V2 while 500 pg (n = 2), 250 pg (n = 2), 100 pg (n = 2), 50 pg (n = 2) and 10 pg (n = 2) were used as input for the WT-Ovation One-Direct system (NuGen Technologies, Inc). All cDNA reactions were purified via Zymo Research Clean and Concentrator™-25 or Qiagen RNeasy MinElute Cleanup kits (WT-Ovation FFPE V2 and WT-Ovation One-Direct systems respectively) as recommended. All purified cDNA probes were assessed for quantity and quality using the Agilent 2100 Bioanalyzer and the Nanodrop-8000 RNA <t>Nano</t> chips. FL-Ovation™ cDNA Biotin Module V2 (NuGEN) was used for fragmentation and biotin labelling of 5 µg of cDNA and used for subsequent hybridisation to Affymetrix HGU133 Plus 2.0 microarrays.
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    Experimental workflow to assess efficiency of NuGen probe generation technologies using low amounts of input <t>RNA.</t> HUVEC total RNA was titrated to cover a range of input RNA from 50 ng–10 pg. 50 ng (n = 1), 500 pg (n = 2) and 250 pg (n = 2) of total RNA was used as input for the WT-Ovation FFPE system V2 while 500 pg (n = 2), 250 pg (n = 2), 100 pg (n = 2), 50 pg (n = 2) and 10 pg (n = 2) were used as input for the WT-Ovation One-Direct system (NuGen Technologies, Inc). All cDNA reactions were purified via Zymo Research Clean and Concentrator™-25 or Qiagen RNeasy MinElute Cleanup kits (WT-Ovation FFPE V2 and WT-Ovation One-Direct systems respectively) as recommended. All purified cDNA probes were assessed for quantity and quality using the Agilent 2100 Bioanalyzer and the Nanodrop-8000 RNA <t>Nano</t> chips. FL-Ovation™ cDNA Biotin Module V2 (NuGEN) was used for fragmentation and biotin labelling of 5 µg of cDNA and used for subsequent hybridisation to Affymetrix HGU133 Plus 2.0 microarrays.
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    Experimental workflow to assess efficiency of NuGen probe generation technologies using low amounts of input <t>RNA.</t> HUVEC total RNA was titrated to cover a range of input RNA from 50 ng–10 pg. 50 ng (n = 1), 500 pg (n = 2) and 250 pg (n = 2) of total RNA was used as input for the WT-Ovation FFPE system V2 while 500 pg (n = 2), 250 pg (n = 2), 100 pg (n = 2), 50 pg (n = 2) and 10 pg (n = 2) were used as input for the WT-Ovation One-Direct system (NuGen Technologies, Inc). All cDNA reactions were purified via Zymo Research Clean and Concentrator™-25 or Qiagen RNeasy MinElute Cleanup kits (WT-Ovation FFPE V2 and WT-Ovation One-Direct systems respectively) as recommended. All purified cDNA probes were assessed for quantity and quality using the Agilent 2100 Bioanalyzer and the Nanodrop-8000 RNA <t>Nano</t> chips. FL-Ovation™ cDNA Biotin Module V2 (NuGEN) was used for fragmentation and biotin labelling of 5 µg of cDNA and used for subsequent hybridisation to Affymetrix HGU133 Plus 2.0 microarrays.
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    Transcriptome analysis of cdKO embryos. <t>RNA</t> was isolated from four wild-type and eight cdKO embryos and analyzed by RNA-seq. (A) Principal-component analysis of the RNA-seq data. The three numbered points correspond to the three numbered embryos shown
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    Image Search Results


    Effect of Filamin A and actin cytoskeleton on RNase L activity. M2 stable cell lines expressing Myc-Filamin A and/or Flag-RNase L WT or Flag-RNase L R667A were analyzed for expression of RNase L and Filamin A on immunoblots (A) and transfected with 10 µM 2-5A (B), and RNase L-mediated cleavage of rRNA (arrows) was analyzed on RNA chips using an Agilent 2100 Bioanalyzer. (C) HT1080 cells were pretreated with Cytochalasin D (Cyto D; 5 µM), Latrunculin A (Lat A; 0.5 µM), or vehicle (DMSO) for 1 h followed by transfection with 10 µM 2-5A or 2 µg/ml of poly(I·C). Characteristic RNase L-generated rRNA cleavage products (arrows) were detected as described for panel B. Representative images from three independent experiments are shown.

    Journal: mBio

    Article Title: RNase L Interacts with Filamin A To Regulate Actin Dynamics and Barrier Function for Viral Entry

    doi: 10.1128/mBio.02012-14

    Figure Lengend Snippet: Effect of Filamin A and actin cytoskeleton on RNase L activity. M2 stable cell lines expressing Myc-Filamin A and/or Flag-RNase L WT or Flag-RNase L R667A were analyzed for expression of RNase L and Filamin A on immunoblots (A) and transfected with 10 µM 2-5A (B), and RNase L-mediated cleavage of rRNA (arrows) was analyzed on RNA chips using an Agilent 2100 Bioanalyzer. (C) HT1080 cells were pretreated with Cytochalasin D (Cyto D; 5 µM), Latrunculin A (Lat A; 0.5 µM), or vehicle (DMSO) for 1 h followed by transfection with 10 µM 2-5A or 2 µg/ml of poly(I·C). Characteristic RNase L-generated rRNA cleavage products (arrows) were detected as described for panel B. Representative images from three independent experiments are shown.

    Article Snippet: SUPPLEMENTAL MATERIAL (A) Expression levels of endogenous RNase L and Filamin A in A7, M2 melanoma, and HT1080 cells normalized to β-actin levels. (B) M2 and A7 melanoma cells were transfected with 10 µM 2-5A or 2 µg/ml of poly(I·C), and specific cleavage of rRNA (shown by arrows) was analyzed on RNA chips using an Agilent 2100 Bioanalyzer.

    Techniques: Activity Assay, Stable Transfection, Expressing, Western Blot, Transfection, Generated

    RNA quality of equine EDTA blood samples. Amount of miRNA (Bioanalyzer, small RNA chip) derived from the same short-term stored sample extracted with PAXgene blood RNA ( a ) or miRNA ( b ) kit. RNA quality (Fragment Analyzer) of long-term stored samples (11 years) with ( c ) or without ( d ) phase separation extracted with PAXgene blood RNA kit

    Journal: Acta Veterinaria Scandinavica

    Article Title: Optimized methods for extracting circulating small RNAs from long-term stored equine samples

    doi: 10.1186/s13028-016-0224-5

    Figure Lengend Snippet: RNA quality of equine EDTA blood samples. Amount of miRNA (Bioanalyzer, small RNA chip) derived from the same short-term stored sample extracted with PAXgene blood RNA ( a ) or miRNA ( b ) kit. RNA quality (Fragment Analyzer) of long-term stored samples (11 years) with ( c ) or without ( d ) phase separation extracted with PAXgene blood RNA kit

    Article Snippet: Samples extracted with both kits did not show significantly different RNA concentration (Wilcoxon signed rank test P = 0.16) whereas the miRNA in small RNA ratio (Agilent 2100 Bioanalyzer) was increased if the PAXgene blood miRNA kit was used (Fig. a, b).

    Techniques: Chromatin Immunoprecipitation, Derivative Assay

    Experimental design for evaluation of reproducibility and limit of detection (LOD) of eWGS. To evaluate reproducibility of results, individual indexed libraries were prepared from 16 samples of defined HPV composition on two occasions 10 days apart (Experiments 1 and 2 in Fig. 1) resulting in 2 pooled libraries. Each library was enriched through hybridization with HPV RNA bait and each enriched library was sequenced on two flow cells. Thus 4 replicate results were obtained for each sample, encompassing experimental replicates (reproducibility of producing enriched library) and sequencing replicates (1a, 1b and 2a, 2b). As each defined sample was a pool of 4 to 5 HPV types with copy number ranging from 625 to 1 (composition shown in Table 1 ), the limit of detection could be assessed from the replicate results

    Journal: BMC Genomics

    Article Title: Universal human papillomavirus typing by whole genome sequencing following target enrichment: evaluation of assay reproducibility and limit of detection

    doi: 10.1186/s12864-019-5598-0

    Figure Lengend Snippet: Experimental design for evaluation of reproducibility and limit of detection (LOD) of eWGS. To evaluate reproducibility of results, individual indexed libraries were prepared from 16 samples of defined HPV composition on two occasions 10 days apart (Experiments 1 and 2 in Fig. 1) resulting in 2 pooled libraries. Each library was enriched through hybridization with HPV RNA bait and each enriched library was sequenced on two flow cells. Thus 4 replicate results were obtained for each sample, encompassing experimental replicates (reproducibility of producing enriched library) and sequencing replicates (1a, 1b and 2a, 2b). As each defined sample was a pool of 4 to 5 HPV types with copy number ranging from 625 to 1 (composition shown in Table 1 ), the limit of detection could be assessed from the replicate results

    Article Snippet: Our original eWGS report provided details on the method [target enrichment with RNA baits (based on Agilent SureSelect technology), library preparation, and sequencing using Illumina HiSeq 2500 platform] as well as initial performance metrics for HPV type determination such as genome coverage and uniformity, but reproducibility and limit of detection (LOD) were not addressed.

    Techniques: Hybridization, Flow Cytometry, Sequencing

    Representative TapeStation electropherograms of total RNA extracted from ARD and post-ARD animals. Arrow marks high molecular weight species, presumably unprocessed rRNA precursor, accumulating in the presence of FUDR. Samples were prepared as in Figure 5A .

    Journal: eLife

    Article Title: Reactivation of RNA metabolism underlies somatic restoration after adult reproductive diapause in C. elegans

    doi: 10.7554/eLife.36194

    Figure Lengend Snippet: Representative TapeStation electropherograms of total RNA extracted from ARD and post-ARD animals. Arrow marks high molecular weight species, presumably unprocessed rRNA precursor, accumulating in the presence of FUDR. Samples were prepared as in Figure 5A .

    Article Snippet: Total RNA, rRNA content, as well as RNA profiles were then analyzed using the Agilent 4200 TapeStation System using High Sensititive RNA ScreenTape following manufacturer protocols.

    Techniques: Molecular Weight

    Exosomal (exo)-miRNAs expression in plasma samples. ( A ) Results of capillary electrophoresis, performed with the small RNA assay (Agilent 2100 Bioanalyzer), show the adequate exo-miRNAs profile of a representative NB patient and an unsuitable exo-miRNAs profile. The miRNAs region goes from 10 to 50 nucleotides included between the dotted lines. The Y-axis represents the fluorescence units [FU] and the X-axis reports the length of RNA molecules in nucleotides [nt]. (B ) The bar chart shows the average number of detected miRNAs (CT value

    Journal: Cancers

    Article Title: Exosomal microRNAs from Longitudinal Liquid Biopsies for the Prediction of Response to Induction Chemotherapy in High-Risk Neuroblastoma Patients: A Proof of Concept SIOPEN Study ‖

    doi: 10.3390/cancers11101476

    Figure Lengend Snippet: Exosomal (exo)-miRNAs expression in plasma samples. ( A ) Results of capillary electrophoresis, performed with the small RNA assay (Agilent 2100 Bioanalyzer), show the adequate exo-miRNAs profile of a representative NB patient and an unsuitable exo-miRNAs profile. The miRNAs region goes from 10 to 50 nucleotides included between the dotted lines. The Y-axis represents the fluorescence units [FU] and the X-axis reports the length of RNA molecules in nucleotides [nt]. (B ) The bar chart shows the average number of detected miRNAs (CT value

    Article Snippet: Quality control and miRNAs evaluation were carried out on the Agilent 2100 Bioanalyzer, using the small RNA assay (Agilent Technologies Spa, Milan, Italy).

    Techniques: Expressing, Electrophoresis, Fluorescence

    Experimental workflow to assess efficiency of NuGen probe generation technologies using low amounts of input RNA. HUVEC total RNA was titrated to cover a range of input RNA from 50 ng–10 pg. 50 ng (n = 1), 500 pg (n = 2) and 250 pg (n = 2) of total RNA was used as input for the WT-Ovation FFPE system V2 while 500 pg (n = 2), 250 pg (n = 2), 100 pg (n = 2), 50 pg (n = 2) and 10 pg (n = 2) were used as input for the WT-Ovation One-Direct system (NuGen Technologies, Inc). All cDNA reactions were purified via Zymo Research Clean and Concentrator™-25 or Qiagen RNeasy MinElute Cleanup kits (WT-Ovation FFPE V2 and WT-Ovation One-Direct systems respectively) as recommended. All purified cDNA probes were assessed for quantity and quality using the Agilent 2100 Bioanalyzer and the Nanodrop-8000 RNA Nano chips. FL-Ovation™ cDNA Biotin Module V2 (NuGEN) was used for fragmentation and biotin labelling of 5 µg of cDNA and used for subsequent hybridisation to Affymetrix HGU133 Plus 2.0 microarrays.

    Journal: PLoS ONE

    Article Title: Global Array-Based Transcriptomics from Minimal Input RNA Utilising an Optimal RNA Isolation Process Combined with SPIA cDNA Probes

    doi: 10.1371/journal.pone.0017625

    Figure Lengend Snippet: Experimental workflow to assess efficiency of NuGen probe generation technologies using low amounts of input RNA. HUVEC total RNA was titrated to cover a range of input RNA from 50 ng–10 pg. 50 ng (n = 1), 500 pg (n = 2) and 250 pg (n = 2) of total RNA was used as input for the WT-Ovation FFPE system V2 while 500 pg (n = 2), 250 pg (n = 2), 100 pg (n = 2), 50 pg (n = 2) and 10 pg (n = 2) were used as input for the WT-Ovation One-Direct system (NuGen Technologies, Inc). All cDNA reactions were purified via Zymo Research Clean and Concentrator™-25 or Qiagen RNeasy MinElute Cleanup kits (WT-Ovation FFPE V2 and WT-Ovation One-Direct systems respectively) as recommended. All purified cDNA probes were assessed for quantity and quality using the Agilent 2100 Bioanalyzer and the Nanodrop-8000 RNA Nano chips. FL-Ovation™ cDNA Biotin Module V2 (NuGEN) was used for fragmentation and biotin labelling of 5 µg of cDNA and used for subsequent hybridisation to Affymetrix HGU133 Plus 2.0 microarrays.

    Article Snippet: The quality of the cDNA probes was assessed before and after fragmentation with the Agilent 2100 Bioanalyzer using RNA Nano chips (Agilent Technologies, Santa Clara, CA, USA).

    Techniques: Formalin-fixed Paraffin-Embedded, Purification, Hybridization

    Transcriptome analysis of cdKO embryos. RNA was isolated from four wild-type and eight cdKO embryos and analyzed by RNA-seq. (A) Principal-component analysis of the RNA-seq data. The three numbered points correspond to the three numbered embryos shown

    Journal: Molecular and Cellular Biology

    Article Title: Tgif1 and Tgif2 Repress Expression of the RabGAP Evi5l

    doi: 10.1128/MCB.00527-16

    Figure Lengend Snippet: Transcriptome analysis of cdKO embryos. RNA was isolated from four wild-type and eight cdKO embryos and analyzed by RNA-seq. (A) Principal-component analysis of the RNA-seq data. The three numbered points correspond to the three numbered embryos shown

    Article Snippet: RNA was isolated and purified using an Absolutely RNA kit (Agilent). cDNA was generated using Superscript III (Invitrogen), and analyzed in triplicate by real-time PCR using a Bio-Rad MyIQ cycler and SensiMix plus SYBR green plus fluorescein isothiocyanate (FITC) mix (Bioline), with intron-spanning primer pairs selected using Primer3 ( ).

    Techniques: Isolation, RNA Sequencing Assay

    Suitability of RNA from LCM samples for gene expression analysis . (A) Aspen: reverse transcriptase-PCR of laser microdissected developing wood fibers of Populus tremula . RNA was amplified using the MessageAmp TM II aRNA kit (ThermoFischer) and then subjected to RT-PCR using actin and ubiquitin specific primers. PCR templates: 1 and 4, cDNA library constructed from Populus tremula x tremuloides developing wood total RNA; 2 and 5, cDNA library constructed from laser microdissected aspen fiber cells (starting from 4 ng of RNA); 3 and 6, cDNA library constructed from laser microdissected fiber cells (starting from 6 ng of RNA). PCR primers: 1 to 3, ACTIN ; 4 to 6, UBIQUITIN . L, 1 kb DNA ladder. PCR 35 cycles. (B) RNA sequencing, spruce: representative quality scores across all bases (Sanger/Illumina 1.9 encoding) for xylem tracheids collected by LCM. Y-axis – quality scores; the higher the score the better the base call. Green area – very good quality calls, orange – reasonable quality calls, red – calls of poor quality. Red line – median value, blue line – mean quality.

    Journal: Frontiers in Plant Science

    Article Title: Laser Capture Microdissection Protocol for Xylem Tissues of Woody Plants

    doi: 10.3389/fpls.2016.01965

    Figure Lengend Snippet: Suitability of RNA from LCM samples for gene expression analysis . (A) Aspen: reverse transcriptase-PCR of laser microdissected developing wood fibers of Populus tremula . RNA was amplified using the MessageAmp TM II aRNA kit (ThermoFischer) and then subjected to RT-PCR using actin and ubiquitin specific primers. PCR templates: 1 and 4, cDNA library constructed from Populus tremula x tremuloides developing wood total RNA; 2 and 5, cDNA library constructed from laser microdissected aspen fiber cells (starting from 4 ng of RNA); 3 and 6, cDNA library constructed from laser microdissected fiber cells (starting from 6 ng of RNA). PCR primers: 1 to 3, ACTIN ; 4 to 6, UBIQUITIN . L, 1 kb DNA ladder. PCR 35 cycles. (B) RNA sequencing, spruce: representative quality scores across all bases (Sanger/Illumina 1.9 encoding) for xylem tracheids collected by LCM. Y-axis – quality scores; the higher the score the better the base call. Green area – very good quality calls, orange – reasonable quality calls, red – calls of poor quality. Red line – median value, blue line – mean quality.

    Article Snippet: Equipment and Reagents RNA extraction kit for LCM material/small samples (RNAqueous® Micro Kit, AMBION) (aspen); RNeasy® Plant Mini Kit (QIAGEN, 74904) (spruce); RNase free water (AMBION AM9930 (aspen); QIAGEN (spruce)); RNA assay kit (AGILENT RNA 6000 PICO KIT 5067-1513); Agilent 2100 G2938A Bioanalyzer for RNA quantitation and quality assessment.

    Techniques: Laser Capture Microdissection, Expressing, Polymerase Chain Reaction, Amplification, Reverse Transcription Polymerase Chain Reaction, cDNA Library Assay, Construct, RNA Sequencing Assay