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  • 99
    Agilent technologies rna 6000 pico kit
    Bioanalyser-based analysis of RNA isolated from virions and dense bodies (DBs). To examine the small RNA species in virions (a) and DBs (b), a small RNA analysis kit (Agilent) was used. Representative electropherograms of RNA from virions and DBs show both miRNAs and small RNAs. (c) Mean percentages of miRNAs in virions and DBs; values are mean± sem of three experiments performed in triplicate. To inspect the total RNA species in virions (d) and DBs (e), an Agilent <t>RNA</t> 6000 Pico kit was used. Representative electropherograms of RNA from virions (d) and DBs (e) show very little 18S and almost no 28S rRNA, in contrast to the uninfected (f) and infected (g) MRC-5 cells. FU, fluorescence units; nt, number of nucleotides. Representative data from three experiments performed in triplicate are shown.
    Rna 6000 Pico Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 3356 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Agilent technologies rna 6000 pico labchip kit
    Impact of the freezing system on morphology of fresh mammary tissue sections and on quality of RNA extracted . Alveolar (acini) structures lined by MECs (yellow arrows) can be easily distinguished after staining mammary tissue sections with Cresyl violet AMBION. Immediately after collection, mammary tissue samples were washed in cold PBS solution, cut in cube of 3-4 mm thickness, and frozen in four different conditions: pieces of mammary tissue were either directly introduced into 1.5-ml eppendorf tubes and frozen in liquid nitrogen (A), or in cold isopentane (-80°C) using the SnapFrost™ system (C), or embedded in OCT ® contained in cryomold before to be immediately immerged in liquid nitrogen (B) or in cold isopentane (D). RNA quality (RIN) which was estimated by <t>RNA</t> 6000 Pico <t>LabChip</t> kit and Agilent 2100 Bioanalyzer, was identical (ranging between 8.5 and 9.5) whatever the freezing procedure, as illustrated in the electrophoreris profiles. Some large blisters (red arrows on Figure A and B) appear however in biopsy flash frozen in liquid nitrogen, mainly without cryoprotector, and morphological details are better seen on tissues frozen using the SnapFrost™ system (Magnification: ×60). The green arrow indicates the thermoplastic film stuck on epithelial cells to be captured.
    Rna 6000 Pico Labchip Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 358 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 358 article reviews
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    rna 6000 pico labchip kit - by Bioz Stars, 2020-01
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    93
    Agilent technologies rna 6000 pico assay kit
    Impact of the freezing system on morphology of fresh mammary tissue sections and on quality of RNA extracted . Alveolar (acini) structures lined by MECs (yellow arrows) can be easily distinguished after staining mammary tissue sections with Cresyl violet AMBION. Immediately after collection, mammary tissue samples were washed in cold PBS solution, cut in cube of 3-4 mm thickness, and frozen in four different conditions: pieces of mammary tissue were either directly introduced into 1.5-ml eppendorf tubes and frozen in liquid nitrogen (A), or in cold isopentane (-80°C) using the SnapFrost™ system (C), or embedded in OCT ® contained in cryomold before to be immediately immerged in liquid nitrogen (B) or in cold isopentane (D). RNA quality (RIN) which was estimated by <t>RNA</t> 6000 Pico <t>LabChip</t> kit and Agilent 2100 Bioanalyzer, was identical (ranging between 8.5 and 9.5) whatever the freezing procedure, as illustrated in the electrophoreris profiles. Some large blisters (red arrows on Figure A and B) appear however in biopsy flash frozen in liquid nitrogen, mainly without cryoprotector, and morphological details are better seen on tissues frozen using the SnapFrost™ system (Magnification: ×60). The green arrow indicates the thermoplastic film stuck on epithelial cells to be captured.
    Rna 6000 Pico Assay Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Agilent technologies rna 6000 pico reagent kit
    Impact of the freezing system on morphology of fresh mammary tissue sections and on quality of RNA extracted . Alveolar (acini) structures lined by MECs (yellow arrows) can be easily distinguished after staining mammary tissue sections with Cresyl violet AMBION. Immediately after collection, mammary tissue samples were washed in cold PBS solution, cut in cube of 3-4 mm thickness, and frozen in four different conditions: pieces of mammary tissue were either directly introduced into 1.5-ml eppendorf tubes and frozen in liquid nitrogen (A), or in cold isopentane (-80°C) using the SnapFrost™ system (C), or embedded in OCT ® contained in cryomold before to be immediately immerged in liquid nitrogen (B) or in cold isopentane (D). RNA quality (RIN) which was estimated by <t>RNA</t> 6000 Pico <t>LabChip</t> kit and Agilent 2100 Bioanalyzer, was identical (ranging between 8.5 and 9.5) whatever the freezing procedure, as illustrated in the electrophoreris profiles. Some large blisters (red arrows on Figure A and B) appear however in biopsy flash frozen in liquid nitrogen, mainly without cryoprotector, and morphological details are better seen on tissues frozen using the SnapFrost™ system (Magnification: ×60). The green arrow indicates the thermoplastic film stuck on epithelial cells to be captured.
    Rna 6000 Pico Reagent Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Agilent technologies bioanalyser rna 6000 pico kit
    Impact of the freezing system on morphology of fresh mammary tissue sections and on quality of RNA extracted . Alveolar (acini) structures lined by MECs (yellow arrows) can be easily distinguished after staining mammary tissue sections with Cresyl violet AMBION. Immediately after collection, mammary tissue samples were washed in cold PBS solution, cut in cube of 3-4 mm thickness, and frozen in four different conditions: pieces of mammary tissue were either directly introduced into 1.5-ml eppendorf tubes and frozen in liquid nitrogen (A), or in cold isopentane (-80°C) using the SnapFrost™ system (C), or embedded in OCT ® contained in cryomold before to be immediately immerged in liquid nitrogen (B) or in cold isopentane (D). RNA quality (RIN) which was estimated by <t>RNA</t> 6000 Pico <t>LabChip</t> kit and Agilent 2100 Bioanalyzer, was identical (ranging between 8.5 and 9.5) whatever the freezing procedure, as illustrated in the electrophoreris profiles. Some large blisters (red arrows on Figure A and B) appear however in biopsy flash frozen in liquid nitrogen, mainly without cryoprotector, and morphological details are better seen on tissues frozen using the SnapFrost™ system (Magnification: ×60). The green arrow indicates the thermoplastic film stuck on epithelial cells to be captured.
    Bioanalyser Rna 6000 Pico Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Agilent technologies rna 6000 nano pico kit
    Impact of the freezing system on morphology of fresh mammary tissue sections and on quality of RNA extracted . Alveolar (acini) structures lined by MECs (yellow arrows) can be easily distinguished after staining mammary tissue sections with Cresyl violet AMBION. Immediately after collection, mammary tissue samples were washed in cold PBS solution, cut in cube of 3-4 mm thickness, and frozen in four different conditions: pieces of mammary tissue were either directly introduced into 1.5-ml eppendorf tubes and frozen in liquid nitrogen (A), or in cold isopentane (-80°C) using the SnapFrost™ system (C), or embedded in OCT ® contained in cryomold before to be immediately immerged in liquid nitrogen (B) or in cold isopentane (D). RNA quality (RIN) which was estimated by <t>RNA</t> 6000 Pico <t>LabChip</t> kit and Agilent 2100 Bioanalyzer, was identical (ranging between 8.5 and 9.5) whatever the freezing procedure, as illustrated in the electrophoreris profiles. Some large blisters (red arrows on Figure A and B) appear however in biopsy flash frozen in liquid nitrogen, mainly without cryoprotector, and morphological details are better seen on tissues frozen using the SnapFrost™ system (Magnification: ×60). The green arrow indicates the thermoplastic film stuck on epithelial cells to be captured.
    Rna 6000 Nano Pico Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 83/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Agilent technologies 6000 pico kit
    Impact of the freezing system on morphology of fresh mammary tissue sections and on quality of RNA extracted . Alveolar (acini) structures lined by MECs (yellow arrows) can be easily distinguished after staining mammary tissue sections with Cresyl violet AMBION. Immediately after collection, mammary tissue samples were washed in cold PBS solution, cut in cube of 3-4 mm thickness, and frozen in four different conditions: pieces of mammary tissue were either directly introduced into 1.5-ml eppendorf tubes and frozen in liquid nitrogen (A), or in cold isopentane (-80°C) using the SnapFrost™ system (C), or embedded in OCT ® contained in cryomold before to be immediately immerged in liquid nitrogen (B) or in cold isopentane (D). RNA quality (RIN) which was estimated by <t>RNA</t> 6000 Pico <t>LabChip</t> kit and Agilent 2100 Bioanalyzer, was identical (ranging between 8.5 and 9.5) whatever the freezing procedure, as illustrated in the electrophoreris profiles. Some large blisters (red arrows on Figure A and B) appear however in biopsy flash frozen in liquid nitrogen, mainly without cryoprotector, and morphological details are better seen on tissues frozen using the SnapFrost™ system (Magnification: ×60). The green arrow indicates the thermoplastic film stuck on epithelial cells to be captured.
    6000 Pico Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Agilent technologies rna pico 6000 microfluidics kit
    Impact of the freezing system on morphology of fresh mammary tissue sections and on quality of RNA extracted . Alveolar (acini) structures lined by MECs (yellow arrows) can be easily distinguished after staining mammary tissue sections with Cresyl violet AMBION. Immediately after collection, mammary tissue samples were washed in cold PBS solution, cut in cube of 3-4 mm thickness, and frozen in four different conditions: pieces of mammary tissue were either directly introduced into 1.5-ml eppendorf tubes and frozen in liquid nitrogen (A), or in cold isopentane (-80°C) using the SnapFrost™ system (C), or embedded in OCT ® contained in cryomold before to be immediately immerged in liquid nitrogen (B) or in cold isopentane (D). RNA quality (RIN) which was estimated by <t>RNA</t> 6000 Pico <t>LabChip</t> kit and Agilent 2100 Bioanalyzer, was identical (ranging between 8.5 and 9.5) whatever the freezing procedure, as illustrated in the electrophoreris profiles. Some large blisters (red arrows on Figure A and B) appear however in biopsy flash frozen in liquid nitrogen, mainly without cryoprotector, and morphological details are better seen on tissues frozen using the SnapFrost™ system (Magnification: ×60). The green arrow indicates the thermoplastic film stuck on epithelial cells to be captured.
    Rna Pico 6000 Microfluidics Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Agilent technologies rna 6000 bioanalyzer pico kit
    Impact of the freezing system on morphology of fresh mammary tissue sections and on quality of RNA extracted . Alveolar (acini) structures lined by MECs (yellow arrows) can be easily distinguished after staining mammary tissue sections with Cresyl violet AMBION. Immediately after collection, mammary tissue samples were washed in cold PBS solution, cut in cube of 3-4 mm thickness, and frozen in four different conditions: pieces of mammary tissue were either directly introduced into 1.5-ml eppendorf tubes and frozen in liquid nitrogen (A), or in cold isopentane (-80°C) using the SnapFrost™ system (C), or embedded in OCT ® contained in cryomold before to be immediately immerged in liquid nitrogen (B) or in cold isopentane (D). RNA quality (RIN) which was estimated by <t>RNA</t> 6000 Pico <t>LabChip</t> kit and Agilent 2100 Bioanalyzer, was identical (ranging between 8.5 and 9.5) whatever the freezing procedure, as illustrated in the electrophoreris profiles. Some large blisters (red arrows on Figure A and B) appear however in biopsy flash frozen in liquid nitrogen, mainly without cryoprotector, and morphological details are better seen on tissues frozen using the SnapFrost™ system (Magnification: ×60). The green arrow indicates the thermoplastic film stuck on epithelial cells to be captured.
    Rna 6000 Bioanalyzer Pico Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 87/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Agilent technologies rna 6000 pico chip kit
    Total RNA profiles for human and bovine MEV and cream fractions. Total RNA contained in fluff (red) and serum (green) MEV fractions were isolated from SEC F1/S1 peaks concentrated by reverse osmosis. Cream was sampled from the first centrifugation of raw milk. RNA extracts were analysed on Agilent <t>RNA</t> 6000 Pico chips using an Agilent Bioanalyzer. The total MEV RNA content was related to the initial start volume of milk (ng ml –1 milk). The peak at 25 nt is an internal standard. Abbreviations: FU, fluorescence units; nt, nucleotides; hF1, human fluff MEV; hS1, human milk serum MEV; bF1, bovine fluff MEV; bS1, bovine milk serum MEV.
    Rna 6000 Pico Chip Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Agilent technologies rna 6000 pico nano labchip kit
    Total RNA profiles for human and bovine MEV and cream fractions. Total RNA contained in fluff (red) and serum (green) MEV fractions were isolated from SEC F1/S1 peaks concentrated by reverse osmosis. Cream was sampled from the first centrifugation of raw milk. RNA extracts were analysed on Agilent <t>RNA</t> 6000 Pico chips using an Agilent Bioanalyzer. The total MEV RNA content was related to the initial start volume of milk (ng ml –1 milk). The peak at 25 nt is an internal standard. Abbreviations: FU, fluorescence units; nt, nucleotides; hF1, human fluff MEV; hS1, human milk serum MEV; bF1, bovine fluff MEV; bS1, bovine milk serum MEV.
    Rna 6000 Pico Nano Labchip Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Agilent technologies rna 6000 pico total rna kit
    Total RNA profiles for human and bovine MEV and cream fractions. Total RNA contained in fluff (red) and serum (green) MEV fractions were isolated from SEC F1/S1 peaks concentrated by reverse osmosis. Cream was sampled from the first centrifugation of raw milk. RNA extracts were analysed on Agilent <t>RNA</t> 6000 Pico chips using an Agilent Bioanalyzer. The total MEV RNA content was related to the initial start volume of milk (ng ml –1 milk). The peak at 25 nt is an internal standard. Abbreviations: FU, fluorescence units; nt, nucleotides; hF1, human fluff MEV; hS1, human milk serum MEV; bF1, bovine fluff MEV; bS1, bovine milk serum MEV.
    Rna 6000 Pico Total Rna Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 80/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Agilent technologies 2100 bioanalyzer rna 6000 pico kit
    Total RNA profiles for human and bovine MEV and cream fractions. Total RNA contained in fluff (red) and serum (green) MEV fractions were isolated from SEC F1/S1 peaks concentrated by reverse osmosis. Cream was sampled from the first centrifugation of raw milk. RNA extracts were analysed on Agilent <t>RNA</t> 6000 Pico chips using an Agilent Bioanalyzer. The total MEV RNA content was related to the initial start volume of milk (ng ml –1 milk). The peak at 25 nt is an internal standard. Abbreviations: FU, fluorescence units; nt, nucleotides; hF1, human fluff MEV; hS1, human milk serum MEV; bF1, bovine fluff MEV; bS1, bovine milk serum MEV.
    2100 Bioanalyzer Rna 6000 Pico Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Agilent technologies series ii rna 6000 pico kit
    Total RNA profiles for human and bovine MEV and cream fractions. Total RNA contained in fluff (red) and serum (green) MEV fractions were isolated from SEC F1/S1 peaks concentrated by reverse osmosis. Cream was sampled from the first centrifugation of raw milk. RNA extracts were analysed on Agilent <t>RNA</t> 6000 Pico chips using an Agilent Bioanalyzer. The total MEV RNA content was related to the initial start volume of milk (ng ml –1 milk). The peak at 25 nt is an internal standard. Abbreviations: FU, fluorescence units; nt, nucleotides; hF1, human fluff MEV; hS1, human milk serum MEV; bF1, bovine fluff MEV; bS1, bovine milk serum MEV.
    Series Ii Rna 6000 Pico Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies rna 6000 pico kit protocol
    Total RNA profiles for human and bovine MEV and cream fractions. Total RNA contained in fluff (red) and serum (green) MEV fractions were isolated from SEC F1/S1 peaks concentrated by reverse osmosis. Cream was sampled from the first centrifugation of raw milk. RNA extracts were analysed on Agilent <t>RNA</t> 6000 Pico chips using an Agilent Bioanalyzer. The total MEV RNA content was related to the initial start volume of milk (ng ml –1 milk). The peak at 25 nt is an internal standard. Abbreviations: FU, fluorescence units; nt, nucleotides; hF1, human fluff MEV; hS1, human milk serum MEV; bF1, bovine fluff MEV; bS1, bovine milk serum MEV.
    Rna 6000 Pico Kit Protocol, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies 2000 bioanalyzer rna 6000 pico labchip kit
    Total RNA profiles for human and bovine MEV and cream fractions. Total RNA contained in fluff (red) and serum (green) MEV fractions were isolated from SEC F1/S1 peaks concentrated by reverse osmosis. Cream was sampled from the first centrifugation of raw milk. RNA extracts were analysed on Agilent <t>RNA</t> 6000 Pico chips using an Agilent Bioanalyzer. The total MEV RNA content was related to the initial start volume of milk (ng ml –1 milk). The peak at 25 nt is an internal standard. Abbreviations: FU, fluorescence units; nt, nucleotides; hF1, human fluff MEV; hS1, human milk serum MEV; bF1, bovine fluff MEV; bS1, bovine milk serum MEV.
    2000 Bioanalyzer Rna 6000 Pico Labchip Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies rna 6000 pico labchip kit series ii
    Total RNA profiles for human and bovine MEV and cream fractions. Total RNA contained in fluff (red) and serum (green) MEV fractions were isolated from SEC F1/S1 peaks concentrated by reverse osmosis. Cream was sampled from the first centrifugation of raw milk. RNA extracts were analysed on Agilent <t>RNA</t> 6000 Pico chips using an Agilent Bioanalyzer. The total MEV RNA content was related to the initial start volume of milk (ng ml –1 milk). The peak at 25 nt is an internal standard. Abbreviations: FU, fluorescence units; nt, nucleotides; hF1, human fluff MEV; hS1, human milk serum MEV; bF1, bovine fluff MEV; bS1, bovine milk serum MEV.
    Rna 6000 Pico Labchip Kit Series Ii, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies bioanalyzer 6000 pico kit
    Total RNA profiles for human and bovine MEV and cream fractions. Total RNA contained in fluff (red) and serum (green) MEV fractions were isolated from SEC F1/S1 peaks concentrated by reverse osmosis. Cream was sampled from the first centrifugation of raw milk. RNA extracts were analysed on Agilent <t>RNA</t> 6000 Pico chips using an Agilent Bioanalyzer. The total MEV RNA content was related to the initial start volume of milk (ng ml –1 milk). The peak at 25 nt is an internal standard. Abbreviations: FU, fluorescence units; nt, nucleotides; hF1, human fluff MEV; hS1, human milk serum MEV; bF1, bovine fluff MEV; bS1, bovine milk serum MEV.
    Bioanalyzer 6000 Pico Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 80/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies 2100 bioanalyser rna 6000 pico kit agilent technologies
    Total RNA profiles for human and bovine MEV and cream fractions. Total RNA contained in fluff (red) and serum (green) MEV fractions were isolated from SEC F1/S1 peaks concentrated by reverse osmosis. Cream was sampled from the first centrifugation of raw milk. RNA extracts were analysed on Agilent <t>RNA</t> 6000 Pico chips using an Agilent Bioanalyzer. The total MEV RNA content was related to the initial start volume of milk (ng ml –1 milk). The peak at 25 nt is an internal standard. Abbreviations: FU, fluorescence units; nt, nucleotides; hF1, human fluff MEV; hS1, human milk serum MEV; bF1, bovine fluff MEV; bS1, bovine milk serum MEV.
    2100 Bioanalyser Rna 6000 Pico Kit Agilent Technologies, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Agilent technologies bioanalyzer rna 6000 pico total rna kit
    Total RNA profiles for human and bovine MEV and cream fractions. Total RNA contained in fluff (red) and serum (green) MEV fractions were isolated from SEC F1/S1 peaks concentrated by reverse osmosis. Cream was sampled from the first centrifugation of raw milk. RNA extracts were analysed on Agilent <t>RNA</t> 6000 Pico chips using an Agilent Bioanalyzer. The total MEV RNA content was related to the initial start volume of milk (ng ml –1 milk). The peak at 25 nt is an internal standard. Abbreviations: FU, fluorescence units; nt, nucleotides; hF1, human fluff MEV; hS1, human milk serum MEV; bF1, bovine fluff MEV; bS1, bovine milk serum MEV.
    Bioanalyzer Rna 6000 Pico Total Rna Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Agilent technologies rna assay kit
    Suitability of <t>RNA</t> from LCM samples for gene expression analysis . (A) Aspen: reverse transcriptase-PCR of laser microdissected developing wood fibers of Populus tremula . RNA was amplified using the MessageAmp TM II aRNA <t>kit</t> (ThermoFischer) and then subjected to RT-PCR using actin and ubiquitin specific primers. PCR templates: 1 and 4, cDNA library constructed from Populus tremula x tremuloides developing wood total RNA; 2 and 5, cDNA library constructed from laser microdissected aspen fiber cells (starting from 4 ng of RNA); 3 and 6, cDNA library constructed from laser microdissected fiber cells (starting from 6 ng of RNA). PCR primers: 1 to 3, ACTIN ; 4 to 6, UBIQUITIN . L, 1 kb DNA ladder. PCR 35 cycles. (B) RNA sequencing, spruce: representative quality scores across all bases (Sanger/Illumina 1.9 encoding) for xylem tracheids collected by LCM. Y-axis – quality scores; the higher the score the better the base call. Green area – very good quality calls, orange – reasonable quality calls, red – calls of poor quality. Red line – median value, blue line – mean quality.
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    Agilent technologies agilent bioanalyzer
    Suitability of <t>RNA</t> from LCM samples for gene expression analysis . (A) Aspen: reverse transcriptase-PCR of laser microdissected developing wood fibers of Populus tremula . RNA was amplified using the MessageAmp TM II aRNA <t>kit</t> (ThermoFischer) and then subjected to RT-PCR using actin and ubiquitin specific primers. PCR templates: 1 and 4, cDNA library constructed from Populus tremula x tremuloides developing wood total RNA; 2 and 5, cDNA library constructed from laser microdissected aspen fiber cells (starting from 4 ng of RNA); 3 and 6, cDNA library constructed from laser microdissected fiber cells (starting from 6 ng of RNA). PCR primers: 1 to 3, ACTIN ; 4 to 6, UBIQUITIN . L, 1 kb DNA ladder. PCR 35 cycles. (B) RNA sequencing, spruce: representative quality scores across all bases (Sanger/Illumina 1.9 encoding) for xylem tracheids collected by LCM. Y-axis – quality scores; the higher the score the better the base call. Green area – very good quality calls, orange – reasonable quality calls, red – calls of poor quality. Red line – median value, blue line – mean quality.
    Agilent Bioanalyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 28251 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and <t>2100</t> <t>Bioanalyzer</t> electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
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    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and <t>2100</t> <t>Bioanalyzer</t> electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
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    Agilent technologies agilent bioanalyzer analysis
    (A) <t>Agilent</t> <t>Bioanalyzer</t> analysis (High-sensitivity DNA Kit) of a SPRI-beads-purified DNA library is shown. DNA fragments smaller than 175 bp include library DNAs with very short cDNA inserts, PCR primer dimers, and residual PCR primers. Gel purification
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    (A) <t>Agilent</t> <t>Bioanalyzer</t> analysis (High-sensitivity DNA Kit) of a SPRI-beads-purified DNA library is shown. DNA fragments smaller than 175 bp include library DNAs with very short cDNA inserts, PCR primer dimers, and residual PCR primers. Gel purification
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    (A) <t>Agilent</t> <t>Bioanalyzer</t> analysis (High-sensitivity DNA Kit) of a SPRI-beads-purified DNA library is shown. DNA fragments smaller than 175 bp include library DNAs with very short cDNA inserts, PCR primer dimers, and residual PCR primers. Gel purification
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    (A) <t>Agilent</t> <t>Bioanalyzer</t> analysis (High-sensitivity DNA Kit) of a SPRI-beads-purified DNA library is shown. DNA fragments smaller than 175 bp include library DNAs with very short cDNA inserts, PCR primer dimers, and residual PCR primers. Gel purification
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    Image Search Results


    Bioanalyser-based analysis of RNA isolated from virions and dense bodies (DBs). To examine the small RNA species in virions (a) and DBs (b), a small RNA analysis kit (Agilent) was used. Representative electropherograms of RNA from virions and DBs show both miRNAs and small RNAs. (c) Mean percentages of miRNAs in virions and DBs; values are mean± sem of three experiments performed in triplicate. To inspect the total RNA species in virions (d) and DBs (e), an Agilent RNA 6000 Pico kit was used. Representative electropherograms of RNA from virions (d) and DBs (e) show very little 18S and almost no 28S rRNA, in contrast to the uninfected (f) and infected (g) MRC-5 cells. FU, fluorescence units; nt, number of nucleotides. Representative data from three experiments performed in triplicate are shown.

    Journal: The Journal of General Virology

    Article Title: Human cytomegalovirus microRNAs are carried by virions and dense bodies and are delivered to target cells

    doi: 10.1099/jgv.0.000736

    Figure Lengend Snippet: Bioanalyser-based analysis of RNA isolated from virions and dense bodies (DBs). To examine the small RNA species in virions (a) and DBs (b), a small RNA analysis kit (Agilent) was used. Representative electropherograms of RNA from virions and DBs show both miRNAs and small RNAs. (c) Mean percentages of miRNAs in virions and DBs; values are mean± sem of three experiments performed in triplicate. To inspect the total RNA species in virions (d) and DBs (e), an Agilent RNA 6000 Pico kit was used. Representative electropherograms of RNA from virions (d) and DBs (e) show very little 18S and almost no 28S rRNA, in contrast to the uninfected (f) and infected (g) MRC-5 cells. FU, fluorescence units; nt, number of nucleotides. Representative data from three experiments performed in triplicate are shown.

    Article Snippet: Agilent RNA 6000 Pico kits (range, 25–6000 nt) were used to assess the total RNA species, and determine their length and integrity.

    Techniques: Isolation, Infection, Fluorescence

    Impact of the freezing system on morphology of fresh mammary tissue sections and on quality of RNA extracted . Alveolar (acini) structures lined by MECs (yellow arrows) can be easily distinguished after staining mammary tissue sections with Cresyl violet AMBION. Immediately after collection, mammary tissue samples were washed in cold PBS solution, cut in cube of 3-4 mm thickness, and frozen in four different conditions: pieces of mammary tissue were either directly introduced into 1.5-ml eppendorf tubes and frozen in liquid nitrogen (A), or in cold isopentane (-80°C) using the SnapFrost™ system (C), or embedded in OCT ® contained in cryomold before to be immediately immerged in liquid nitrogen (B) or in cold isopentane (D). RNA quality (RIN) which was estimated by RNA 6000 Pico LabChip kit and Agilent 2100 Bioanalyzer, was identical (ranging between 8.5 and 9.5) whatever the freezing procedure, as illustrated in the electrophoreris profiles. Some large blisters (red arrows on Figure A and B) appear however in biopsy flash frozen in liquid nitrogen, mainly without cryoprotector, and morphological details are better seen on tissues frozen using the SnapFrost™ system (Magnification: ×60). The green arrow indicates the thermoplastic film stuck on epithelial cells to be captured.

    Journal: BMC Cell Biology

    Article Title: Maintaining RNA integrity in a homogeneous population of mammary epithelial cells isolated by Laser Capture Microdissection

    doi: 10.1186/1471-2121-11-95

    Figure Lengend Snippet: Impact of the freezing system on morphology of fresh mammary tissue sections and on quality of RNA extracted . Alveolar (acini) structures lined by MECs (yellow arrows) can be easily distinguished after staining mammary tissue sections with Cresyl violet AMBION. Immediately after collection, mammary tissue samples were washed in cold PBS solution, cut in cube of 3-4 mm thickness, and frozen in four different conditions: pieces of mammary tissue were either directly introduced into 1.5-ml eppendorf tubes and frozen in liquid nitrogen (A), or in cold isopentane (-80°C) using the SnapFrost™ system (C), or embedded in OCT ® contained in cryomold before to be immediately immerged in liquid nitrogen (B) or in cold isopentane (D). RNA quality (RIN) which was estimated by RNA 6000 Pico LabChip kit and Agilent 2100 Bioanalyzer, was identical (ranging between 8.5 and 9.5) whatever the freezing procedure, as illustrated in the electrophoreris profiles. Some large blisters (red arrows on Figure A and B) appear however in biopsy flash frozen in liquid nitrogen, mainly without cryoprotector, and morphological details are better seen on tissues frozen using the SnapFrost™ system (Magnification: ×60). The green arrow indicates the thermoplastic film stuck on epithelial cells to be captured.

    Article Snippet: The fluorimetric method and micro-capillary electrophoresis device developed by Agilent Technologies was chosen to determine RNA concentration and quality with RNA 6000 pico LabChip Kit in the Agilent Bioanalyzer 2100 system.

    Techniques: Staining

    Total RNA profiles for human and bovine MEV and cream fractions. Total RNA contained in fluff (red) and serum (green) MEV fractions were isolated from SEC F1/S1 peaks concentrated by reverse osmosis. Cream was sampled from the first centrifugation of raw milk. RNA extracts were analysed on Agilent RNA 6000 Pico chips using an Agilent Bioanalyzer. The total MEV RNA content was related to the initial start volume of milk (ng ml –1 milk). The peak at 25 nt is an internal standard. Abbreviations: FU, fluorescence units; nt, nucleotides; hF1, human fluff MEV; hS1, human milk serum MEV; bF1, bovine fluff MEV; bS1, bovine milk serum MEV.

    Journal: Journal of Extracellular Vesicles

    Article Title: Pellet-free isolation of human and bovine milk extracellular vesicles by size-exclusion chromatography

    doi: 10.1080/20013078.2017.1294340

    Figure Lengend Snippet: Total RNA profiles for human and bovine MEV and cream fractions. Total RNA contained in fluff (red) and serum (green) MEV fractions were isolated from SEC F1/S1 peaks concentrated by reverse osmosis. Cream was sampled from the first centrifugation of raw milk. RNA extracts were analysed on Agilent RNA 6000 Pico chips using an Agilent Bioanalyzer. The total MEV RNA content was related to the initial start volume of milk (ng ml –1 milk). The peak at 25 nt is an internal standard. Abbreviations: FU, fluorescence units; nt, nucleotides; hF1, human fluff MEV; hS1, human milk serum MEV; bF1, bovine fluff MEV; bS1, bovine milk serum MEV.

    Article Snippet: Total RNA from −80°C preserved samples, including fluff and milk serum MEV isolates obtained from the same milk lot, was isolated using the miRCURYTM RNA Isolation Kit, and analysed in triplicates on a Agilent RNA 6000 Pico kit chip using a Agilent Bioanalyzer ( ).

    Techniques: Isolation, Size-exclusion Chromatography, Centrifugation, Fluorescence

    Suitability of RNA from LCM samples for gene expression analysis . (A) Aspen: reverse transcriptase-PCR of laser microdissected developing wood fibers of Populus tremula . RNA was amplified using the MessageAmp TM II aRNA kit (ThermoFischer) and then subjected to RT-PCR using actin and ubiquitin specific primers. PCR templates: 1 and 4, cDNA library constructed from Populus tremula x tremuloides developing wood total RNA; 2 and 5, cDNA library constructed from laser microdissected aspen fiber cells (starting from 4 ng of RNA); 3 and 6, cDNA library constructed from laser microdissected fiber cells (starting from 6 ng of RNA). PCR primers: 1 to 3, ACTIN ; 4 to 6, UBIQUITIN . L, 1 kb DNA ladder. PCR 35 cycles. (B) RNA sequencing, spruce: representative quality scores across all bases (Sanger/Illumina 1.9 encoding) for xylem tracheids collected by LCM. Y-axis – quality scores; the higher the score the better the base call. Green area – very good quality calls, orange – reasonable quality calls, red – calls of poor quality. Red line – median value, blue line – mean quality.

    Journal: Frontiers in Plant Science

    Article Title: Laser Capture Microdissection Protocol for Xylem Tissues of Woody Plants

    doi: 10.3389/fpls.2016.01965

    Figure Lengend Snippet: Suitability of RNA from LCM samples for gene expression analysis . (A) Aspen: reverse transcriptase-PCR of laser microdissected developing wood fibers of Populus tremula . RNA was amplified using the MessageAmp TM II aRNA kit (ThermoFischer) and then subjected to RT-PCR using actin and ubiquitin specific primers. PCR templates: 1 and 4, cDNA library constructed from Populus tremula x tremuloides developing wood total RNA; 2 and 5, cDNA library constructed from laser microdissected aspen fiber cells (starting from 4 ng of RNA); 3 and 6, cDNA library constructed from laser microdissected fiber cells (starting from 6 ng of RNA). PCR primers: 1 to 3, ACTIN ; 4 to 6, UBIQUITIN . L, 1 kb DNA ladder. PCR 35 cycles. (B) RNA sequencing, spruce: representative quality scores across all bases (Sanger/Illumina 1.9 encoding) for xylem tracheids collected by LCM. Y-axis – quality scores; the higher the score the better the base call. Green area – very good quality calls, orange – reasonable quality calls, red – calls of poor quality. Red line – median value, blue line – mean quality.

    Article Snippet: Equipment and Reagents RNA extraction kit for LCM material/small samples (RNAqueous® Micro Kit, AMBION) (aspen); RNeasy® Plant Mini Kit (QIAGEN, 74904) (spruce); RNase free water (AMBION AM9930 (aspen); QIAGEN (spruce)); RNA assay kit (AGILENT RNA 6000 PICO KIT 5067-1513); Agilent 2100 G2938A Bioanalyzer for RNA quantitation and quality assessment.

    Techniques: Laser Capture Microdissection, Expressing, Polymerase Chain Reaction, Amplification, Reverse Transcription Polymerase Chain Reaction, cDNA Library Assay, Construct, RNA Sequencing Assay

    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and 2100 Bioanalyzer electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.

    Journal: BMC Research Notes

    Article Title: MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits

    doi: 10.1186/1756-0500-4-217

    Figure Lengend Snippet: Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and 2100 Bioanalyzer electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.

    Article Snippet: Determination of RNA concentrations and RNA integrity Quantification of totalRNA yield and assessment of integrity was done with the Experion (Bio-Rad laboratories) and 2100 Bioanalyzer (Agilent systems using the Experion RNA HighSense Analysis Kit and the Agilent RNA 6000 Pico Kit respectively.

    Techniques: Software, Electrophoresis, Standard Deviation

    (A) Agilent Bioanalyzer analysis (High-sensitivity DNA Kit) of a SPRI-beads-purified DNA library is shown. DNA fragments smaller than 175 bp include library DNAs with very short cDNA inserts, PCR primer dimers, and residual PCR primers. Gel purification

    Journal: Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]

    Article Title: Current Protocols in Molecular Biology Unit 4.23

    doi: 10.1002/0471142727.mb0423s110

    Figure Lengend Snippet: (A) Agilent Bioanalyzer analysis (High-sensitivity DNA Kit) of a SPRI-beads-purified DNA library is shown. DNA fragments smaller than 175 bp include library DNAs with very short cDNA inserts, PCR primer dimers, and residual PCR primers. Gel purification

    Article Snippet: Note: the quality and quantity of RNA fragments can be examined by Agilent Bioanalyzer analysis (RNA 6000 Pico Kit).

    Techniques: Purification, Polymerase Chain Reaction, Gel Purification