rlt buffer Search Results


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  • 95
    Qiagen rlt lysis buffer
    Asymmetric competition is dependent on direct interactions between resident and challenger pneumococci. a, Day 4–5 old pups were inoculated with a heat-killed serotype 23F resident strain (10 5 CFU) for 6h before introduction of the live isogenic challenger strain with increasing CFU (10 1 -10 3 ). Nasal lavages were cultured 3 days later to determine challenger colonization densities, n=3–4. Median values are shown. b, Pups were inoculated with live (10 3 CFU) or heat-killed (10 5 CFU) resident strain for 6h and nasal lavages with <t>RLT</t> lysis buffer were obtained to isolate host <t>RNA.</t> Early inflammatory cytokine expression levels were assessed by SYBR green real-time quantitative RT-PCR. Cytokine expression induced by heat-killed S. pneumoniae were shown as fold change relative to that elicited by live bacteria, n=5–6. Data are shown as mean ± SEM. c-d, Pups were inoculated with either a streptomycin sensitive ( c ) or resistant ( d ) resident strain for 3 days before intranasal administration of streptomycin. An isogenic streptomycin resistant challenger was inoculated 6h later and its colonization density determined after 3 days. Median values are shown. Colonization levels by 10 1 CFU challenger alone, and in resident-colonized pups treated with either phosphate buffered saline (PBS) or streptomycin, were compared by Kruskal–Wallis one-way analysis of variance in ( d ), n=4–8, * p
    Rlt Lysis Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 4335 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen buffer rlt plus
    Flow cytometry for isolation of microglia from early adolescent mouse visual cortex. (A) Schematic illustrating the use of fluorescence-activated cell sorting (FACS) to isolate microglia from P28 bilateral V1b. Gating scheme that was used for all samples shows (B) selection of the population containing cells, including microglia; (C) selection of singlets; (D) selection of the propidium iodide (PI) negative population representing live cells; and (E) selection of CD11b+ CD45low microglia that were collected directly into Buffer <t>RLT</t> <t>Plus</t> ™ prior to RNA extraction.
    Buffer Rlt Plus, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 499 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    PEQLAB rlt buffer
    Flow cytometry for isolation of microglia from early adolescent mouse visual cortex. (A) Schematic illustrating the use of fluorescence-activated cell sorting (FACS) to isolate microglia from P28 bilateral V1b. Gating scheme that was used for all samples shows (B) selection of the population containing cells, including microglia; (C) selection of singlets; (D) selection of the propidium iodide (PI) negative population representing live cells; and (E) selection of CD11b+ CD45low microglia that were collected directly into Buffer <t>RLT</t> <t>Plus</t> ™ prior to RNA extraction.
    Rlt Buffer, supplied by PEQLAB, used in various techniques. Bioz Stars score: 99/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega rlt buffer
    Flow cytometry for isolation of microglia from early adolescent mouse visual cortex. (A) Schematic illustrating the use of fluorescence-activated cell sorting (FACS) to isolate microglia from P28 bilateral V1b. Gating scheme that was used for all samples shows (B) selection of the population containing cells, including microglia; (C) selection of singlets; (D) selection of the propidium iodide (PI) negative population representing live cells; and (E) selection of CD11b+ CD45low microglia that were collected directly into Buffer <t>RLT</t> <t>Plus</t> ™ prior to RNA extraction.
    Rlt Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rlt buffer
    Flow cytometry for isolation of microglia from early adolescent mouse visual cortex. (A) Schematic illustrating the use of fluorescence-activated cell sorting (FACS) to isolate microglia from P28 bilateral V1b. Gating scheme that was used for all samples shows (B) selection of the population containing cells, including microglia; (C) selection of singlets; (D) selection of the propidium iodide (PI) negative population representing live cells; and (E) selection of CD11b+ CD45low microglia that were collected directly into Buffer <t>RLT</t> <t>Plus</t> ™ prior to RNA extraction.
    Rlt Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 243 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Bio Basic Canada rlt buffer
    Flow cytometry for isolation of microglia from early adolescent mouse visual cortex. (A) Schematic illustrating the use of fluorescence-activated cell sorting (FACS) to isolate microglia from P28 bilateral V1b. Gating scheme that was used for all samples shows (B) selection of the population containing cells, including microglia; (C) selection of singlets; (D) selection of the propidium iodide (PI) negative population representing live cells; and (E) selection of CD11b+ CD45low microglia that were collected directly into Buffer <t>RLT</t> <t>Plus</t> ™ prior to RNA extraction.
    Rlt Buffer, supplied by Bio Basic Canada, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Eppendorf AG rlt buffer
    Flow cytometry for isolation of microglia from early adolescent mouse visual cortex. (A) Schematic illustrating the use of fluorescence-activated cell sorting (FACS) to isolate microglia from P28 bilateral V1b. Gating scheme that was used for all samples shows (B) selection of the population containing cells, including microglia; (C) selection of singlets; (D) selection of the propidium iodide (PI) negative population representing live cells; and (E) selection of CD11b+ CD45low microglia that were collected directly into Buffer <t>RLT</t> <t>Plus</t> ™ prior to RNA extraction.
    Rlt Buffer, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Roche rlt buffer
    Flow cytometry for isolation of microglia from early adolescent mouse visual cortex. (A) Schematic illustrating the use of fluorescence-activated cell sorting (FACS) to isolate microglia from P28 bilateral V1b. Gating scheme that was used for all samples shows (B) selection of the population containing cells, including microglia; (C) selection of singlets; (D) selection of the propidium iodide (PI) negative population representing live cells; and (E) selection of CD11b+ CD45low microglia that were collected directly into Buffer <t>RLT</t> <t>Plus</t> ™ prior to RNA extraction.
    Rlt Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Bertin Technologies rlt buffer
    Flow cytometry for isolation of microglia from early adolescent mouse visual cortex. (A) Schematic illustrating the use of fluorescence-activated cell sorting (FACS) to isolate microglia from P28 bilateral V1b. Gating scheme that was used for all samples shows (B) selection of the population containing cells, including microglia; (C) selection of singlets; (D) selection of the propidium iodide (PI) negative population representing live cells; and (E) selection of CD11b+ CD45low microglia that were collected directly into Buffer <t>RLT</t> <t>Plus</t> ™ prior to RNA extraction.
    Rlt Buffer, supplied by Bertin Technologies, used in various techniques. Bioz Stars score: 97/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Bio-Rad rlt buffer
    Flow cytometry for isolation of microglia from early adolescent mouse visual cortex. (A) Schematic illustrating the use of fluorescence-activated cell sorting (FACS) to isolate microglia from P28 bilateral V1b. Gating scheme that was used for all samples shows (B) selection of the population containing cells, including microglia; (C) selection of singlets; (D) selection of the propidium iodide (PI) negative population representing live cells; and (E) selection of CD11b+ CD45low microglia that were collected directly into Buffer <t>RLT</t> <t>Plus</t> ™ prior to RNA extraction.
    Rlt Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Sangon Biotech rlt buffer
    Flow cytometry for isolation of microglia from early adolescent mouse visual cortex. (A) Schematic illustrating the use of fluorescence-activated cell sorting (FACS) to isolate microglia from P28 bilateral V1b. Gating scheme that was used for all samples shows (B) selection of the population containing cells, including microglia; (C) selection of singlets; (D) selection of the propidium iodide (PI) negative population representing live cells; and (E) selection of CD11b+ CD45low microglia that were collected directly into Buffer <t>RLT</t> <t>Plus</t> ™ prior to RNA extraction.
    Rlt Buffer, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Millipore buffer rlt β mercaptoethanol
    Flow cytometry for isolation of microglia from early adolescent mouse visual cortex. (A) Schematic illustrating the use of fluorescence-activated cell sorting (FACS) to isolate microglia from P28 bilateral V1b. Gating scheme that was used for all samples shows (B) selection of the population containing cells, including microglia; (C) selection of singlets; (D) selection of the propidium iodide (PI) negative population representing live cells; and (E) selection of CD11b+ CD45low microglia that were collected directly into Buffer <t>RLT</t> <t>Plus</t> ™ prior to RNA extraction.
    Buffer Rlt β Mercaptoethanol, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Qiagen rlt bufffer
    Flow cytometry for isolation of microglia from early adolescent mouse visual cortex. (A) Schematic illustrating the use of fluorescence-activated cell sorting (FACS) to isolate microglia from P28 bilateral V1b. Gating scheme that was used for all samples shows (B) selection of the population containing cells, including microglia; (C) selection of singlets; (D) selection of the propidium iodide (PI) negative population representing live cells; and (E) selection of CD11b+ CD45low microglia that were collected directly into Buffer <t>RLT</t> <t>Plus</t> ™ prior to RNA extraction.
    Rlt Bufffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 87/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Qiagen 500ul rlt buffer
    Flow cytometry for isolation of microglia from early adolescent mouse visual cortex. (A) Schematic illustrating the use of fluorescence-activated cell sorting (FACS) to isolate microglia from P28 bilateral V1b. Gating scheme that was used for all samples shows (B) selection of the population containing cells, including microglia; (C) selection of singlets; (D) selection of the propidium iodide (PI) negative population representing live cells; and (E) selection of CD11b+ CD45low microglia that were collected directly into Buffer <t>RLT</t> <t>Plus</t> ™ prior to RNA extraction.
    500ul Rlt Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Bio-Rad qiagen rlt buffer
    Flow cytometry for isolation of microglia from early adolescent mouse visual cortex. (A) Schematic illustrating the use of fluorescence-activated cell sorting (FACS) to isolate microglia from P28 bilateral V1b. Gating scheme that was used for all samples shows (B) selection of the population containing cells, including microglia; (C) selection of singlets; (D) selection of the propidium iodide (PI) negative population representing live cells; and (E) selection of CD11b+ CD45low microglia that were collected directly into Buffer <t>RLT</t> <t>Plus</t> ™ prior to RNA extraction.
    Qiagen Rlt Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Qiagen rlt βme buffer
    Flow cytometry for isolation of microglia from early adolescent mouse visual cortex. (A) Schematic illustrating the use of fluorescence-activated cell sorting (FACS) to isolate microglia from P28 bilateral V1b. Gating scheme that was used for all samples shows (B) selection of the population containing cells, including microglia; (C) selection of singlets; (D) selection of the propidium iodide (PI) negative population representing live cells; and (E) selection of CD11b+ CD45low microglia that were collected directly into Buffer <t>RLT</t> <t>Plus</t> ™ prior to RNA extraction.
    Rlt βme Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Qiagen 350ul rlt buffer
    Flow cytometry for isolation of microglia from early adolescent mouse visual cortex. (A) Schematic illustrating the use of fluorescence-activated cell sorting (FACS) to isolate microglia from P28 bilateral V1b. Gating scheme that was used for all samples shows (B) selection of the population containing cells, including microglia; (C) selection of singlets; (D) selection of the propidium iodide (PI) negative population representing live cells; and (E) selection of CD11b+ CD45low microglia that were collected directly into Buffer <t>RLT</t> <t>Plus</t> ™ prior to RNA extraction.
    350ul Rlt Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Qiagen rlt β2 me buffer
    Flow cytometry for isolation of microglia from early adolescent mouse visual cortex. (A) Schematic illustrating the use of fluorescence-activated cell sorting (FACS) to isolate microglia from P28 bilateral V1b. Gating scheme that was used for all samples shows (B) selection of the population containing cells, including microglia; (C) selection of singlets; (D) selection of the propidium iodide (PI) negative population representing live cells; and (E) selection of CD11b+ CD45low microglia that were collected directly into Buffer <t>RLT</t> <t>Plus</t> ™ prior to RNA extraction.
    Rlt β2 Me Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    PEQLAB rlt lysis buffer
    Flow cytometry for isolation of microglia from early adolescent mouse visual cortex. (A) Schematic illustrating the use of fluorescence-activated cell sorting (FACS) to isolate microglia from P28 bilateral V1b. Gating scheme that was used for all samples shows (B) selection of the population containing cells, including microglia; (C) selection of singlets; (D) selection of the propidium iodide (PI) negative population representing live cells; and (E) selection of CD11b+ CD45low microglia that were collected directly into Buffer <t>RLT</t> <t>Plus</t> ™ prior to RNA extraction.
    Rlt Lysis Buffer, supplied by PEQLAB, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen rlt buffer β mercaptoethanol buffer
    Flow cytometry for isolation of microglia from early adolescent mouse visual cortex. (A) Schematic illustrating the use of fluorescence-activated cell sorting (FACS) to isolate microglia from P28 bilateral V1b. Gating scheme that was used for all samples shows (B) selection of the population containing cells, including microglia; (C) selection of singlets; (D) selection of the propidium iodide (PI) negative population representing live cells; and (E) selection of CD11b+ CD45low microglia that were collected directly into Buffer <t>RLT</t> <t>Plus</t> ™ prior to RNA extraction.
    Rlt Buffer β Mercaptoethanol Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    BioLegend rlt plus lysis buffer
    Kinetics of changes in the transcription of purinergic genes, cytokines, and transcription factors following activation of cultured human <t>iNKT</t> cells. Cells were collected in <t>RLT</t> lysis buffer (Qiagen) at various times after activation by TCR engagement (5 μg/ml plate-bound αCD3 Ab) and transcripts were quantified by RT-PCR. The results are representative of duplicate experiments. Gene names and corresponding proteins are: ADORA2A , adenosine A2A receptor; CD38 , cyclic ADP-ribose hydrolase 1/NAD + nucleosidase; CD39 , ectonucleoside triphosphate diphosphohydrolase 1; ENPP1 , ectonucleotide pyrophosphatase/phosphodiesterase 1; CD73 , ecto-5-nucleotidase; P2RX7 , P2X7 receptor; PANX1 , pannexin 1; ENT1 , equilibrative nucleoside transporter 1; ADA ( ADA1 ), adenosine deaminase; CD69 , early lymphocyte activation antigen; IFNG , interferon γ; TBX21 ( TBET ), T-box 21; GATA3 , GATA binding protein 3.
    Rlt Plus Lysis Buffer, supplied by BioLegend, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen rneasy mini kit rlt buffer
    Kinetics of changes in the transcription of purinergic genes, cytokines, and transcription factors following activation of cultured human <t>iNKT</t> cells. Cells were collected in <t>RLT</t> lysis buffer (Qiagen) at various times after activation by TCR engagement (5 μg/ml plate-bound αCD3 Ab) and transcripts were quantified by RT-PCR. The results are representative of duplicate experiments. Gene names and corresponding proteins are: ADORA2A , adenosine A2A receptor; CD38 , cyclic ADP-ribose hydrolase 1/NAD + nucleosidase; CD39 , ectonucleoside triphosphate diphosphohydrolase 1; ENPP1 , ectonucleotide pyrophosphatase/phosphodiesterase 1; CD73 , ecto-5-nucleotidase; P2RX7 , P2X7 receptor; PANX1 , pannexin 1; ENT1 , equilibrative nucleoside transporter 1; ADA ( ADA1 ), adenosine deaminase; CD69 , early lymphocyte activation antigen; IFNG , interferon γ; TBX21 ( TBET ), T-box 21; GATA3 , GATA binding protein 3.
    Rneasy Mini Kit Rlt Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Qiagen chaotropic rlt lysis buffer
    Kinetics of changes in the transcription of purinergic genes, cytokines, and transcription factors following activation of cultured human <t>iNKT</t> cells. Cells were collected in <t>RLT</t> lysis buffer (Qiagen) at various times after activation by TCR engagement (5 μg/ml plate-bound αCD3 Ab) and transcripts were quantified by RT-PCR. The results are representative of duplicate experiments. Gene names and corresponding proteins are: ADORA2A , adenosine A2A receptor; CD38 , cyclic ADP-ribose hydrolase 1/NAD + nucleosidase; CD39 , ectonucleoside triphosphate diphosphohydrolase 1; ENPP1 , ectonucleotide pyrophosphatase/phosphodiesterase 1; CD73 , ecto-5-nucleotidase; P2RX7 , P2X7 receptor; PANX1 , pannexin 1; ENT1 , equilibrative nucleoside transporter 1; ADA ( ADA1 ), adenosine deaminase; CD69 , early lymphocyte activation antigen; IFNG , interferon γ; TBX21 ( TBET ), T-box 21; GATA3 , GATA binding protein 3.
    Chaotropic Rlt Lysis Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen guanidinium thiocyanate containing rlt buffer
    Kinetics of changes in the transcription of purinergic genes, cytokines, and transcription factors following activation of cultured human <t>iNKT</t> cells. Cells were collected in <t>RLT</t> lysis buffer (Qiagen) at various times after activation by TCR engagement (5 μg/ml plate-bound αCD3 Ab) and transcripts were quantified by RT-PCR. The results are representative of duplicate experiments. Gene names and corresponding proteins are: ADORA2A , adenosine A2A receptor; CD38 , cyclic ADP-ribose hydrolase 1/NAD + nucleosidase; CD39 , ectonucleoside triphosphate diphosphohydrolase 1; ENPP1 , ectonucleotide pyrophosphatase/phosphodiesterase 1; CD73 , ecto-5-nucleotidase; P2RX7 , P2X7 receptor; PANX1 , pannexin 1; ENT1 , equilibrative nucleoside transporter 1; ADA ( ADA1 ), adenosine deaminase; CD69 , early lymphocyte activation antigen; IFNG , interferon γ; TBX21 ( TBET ), T-box 21; GATA3 , GATA binding protein 3.
    Guanidinium Thiocyanate Containing Rlt Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen guanidium based rlt lysis buffer
    Kinetics of changes in the transcription of purinergic genes, cytokines, and transcription factors following activation of cultured human <t>iNKT</t> cells. Cells were collected in <t>RLT</t> lysis buffer (Qiagen) at various times after activation by TCR engagement (5 μg/ml plate-bound αCD3 Ab) and transcripts were quantified by RT-PCR. The results are representative of duplicate experiments. Gene names and corresponding proteins are: ADORA2A , adenosine A2A receptor; CD38 , cyclic ADP-ribose hydrolase 1/NAD + nucleosidase; CD39 , ectonucleoside triphosphate diphosphohydrolase 1; ENPP1 , ectonucleotide pyrophosphatase/phosphodiesterase 1; CD73 , ecto-5-nucleotidase; P2RX7 , P2X7 receptor; PANX1 , pannexin 1; ENT1 , equilibrative nucleoside transporter 1; ADA ( ADA1 ), adenosine deaminase; CD69 , early lymphocyte activation antigen; IFNG , interferon γ; TBX21 ( TBET ), T-box 21; GATA3 , GATA binding protein 3.
    Guanidium Based Rlt Lysis Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen rlt beta mercaptoethanol lysis buffer
    Kinetics of changes in the transcription of purinergic genes, cytokines, and transcription factors following activation of cultured human <t>iNKT</t> cells. Cells were collected in <t>RLT</t> lysis buffer (Qiagen) at various times after activation by TCR engagement (5 μg/ml plate-bound αCD3 Ab) and transcripts were quantified by RT-PCR. The results are representative of duplicate experiments. Gene names and corresponding proteins are: ADORA2A , adenosine A2A receptor; CD38 , cyclic ADP-ribose hydrolase 1/NAD + nucleosidase; CD39 , ectonucleoside triphosphate diphosphohydrolase 1; ENPP1 , ectonucleotide pyrophosphatase/phosphodiesterase 1; CD73 , ecto-5-nucleotidase; P2RX7 , P2X7 receptor; PANX1 , pannexin 1; ENT1 , equilibrative nucleoside transporter 1; ADA ( ADA1 ), adenosine deaminase; CD69 , early lymphocyte activation antigen; IFNG , interferon γ; TBX21 ( TBET ), T-box 21; GATA3 , GATA binding protein 3.
    Rlt Beta Mercaptoethanol Lysis Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen guanidine isothiocyanate containing rlt buffer
    Kinetics of changes in the transcription of purinergic genes, cytokines, and transcription factors following activation of cultured human <t>iNKT</t> cells. Cells were collected in <t>RLT</t> lysis buffer (Qiagen) at various times after activation by TCR engagement (5 μg/ml plate-bound αCD3 Ab) and transcripts were quantified by RT-PCR. The results are representative of duplicate experiments. Gene names and corresponding proteins are: ADORA2A , adenosine A2A receptor; CD38 , cyclic ADP-ribose hydrolase 1/NAD + nucleosidase; CD39 , ectonucleoside triphosphate diphosphohydrolase 1; ENPP1 , ectonucleotide pyrophosphatase/phosphodiesterase 1; CD73 , ecto-5-nucleotidase; P2RX7 , P2X7 receptor; PANX1 , pannexin 1; ENT1 , equilibrative nucleoside transporter 1; ADA ( ADA1 ), adenosine deaminase; CD69 , early lymphocyte activation antigen; IFNG , interferon γ; TBX21 ( TBET ), T-box 21; GATA3 , GATA binding protein 3.
    Guanidine Isothiocyanate Containing Rlt Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen rlt lysis buffer added β mercaptoethanol bme
    Kinetics of changes in the transcription of purinergic genes, cytokines, and transcription factors following activation of cultured human <t>iNKT</t> cells. Cells were collected in <t>RLT</t> lysis buffer (Qiagen) at various times after activation by TCR engagement (5 μg/ml plate-bound αCD3 Ab) and transcripts were quantified by RT-PCR. The results are representative of duplicate experiments. Gene names and corresponding proteins are: ADORA2A , adenosine A2A receptor; CD38 , cyclic ADP-ribose hydrolase 1/NAD + nucleosidase; CD39 , ectonucleoside triphosphate diphosphohydrolase 1; ENPP1 , ectonucleotide pyrophosphatase/phosphodiesterase 1; CD73 , ecto-5-nucleotidase; P2RX7 , P2X7 receptor; PANX1 , pannexin 1; ENT1 , equilibrative nucleoside transporter 1; ADA ( ADA1 ), adenosine deaminase; CD69 , early lymphocyte activation antigen; IFNG , interferon γ; TBX21 ( TBET ), T-box 21; GATA3 , GATA binding protein 3.
    Rlt Lysis Buffer Added β Mercaptoethanol Bme, supplied by Qiagen, used in various techniques. Bioz Stars score: 82/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio Basic Canada rlt lysis buffer from the ez 10 spin column total rna minipreps super kit bio basic
    Kinetics of changes in the transcription of purinergic genes, cytokines, and transcription factors following activation of cultured human <t>iNKT</t> cells. Cells were collected in <t>RLT</t> lysis buffer (Qiagen) at various times after activation by TCR engagement (5 μg/ml plate-bound αCD3 Ab) and transcripts were quantified by RT-PCR. The results are representative of duplicate experiments. Gene names and corresponding proteins are: ADORA2A , adenosine A2A receptor; CD38 , cyclic ADP-ribose hydrolase 1/NAD + nucleosidase; CD39 , ectonucleoside triphosphate diphosphohydrolase 1; ENPP1 , ectonucleotide pyrophosphatase/phosphodiesterase 1; CD73 , ecto-5-nucleotidase; P2RX7 , P2X7 receptor; PANX1 , pannexin 1; ENT1 , equilibrative nucleoside transporter 1; ADA ( ADA1 ), adenosine deaminase; CD69 , early lymphocyte activation antigen; IFNG , interferon γ; TBX21 ( TBET ), T-box 21; GATA3 , GATA binding protein 3.
    Rlt Lysis Buffer From The Ez 10 Spin Column Total Rna Minipreps Super Kit Bio Basic, supplied by Bio Basic Canada, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Asymmetric competition is dependent on direct interactions between resident and challenger pneumococci. a, Day 4–5 old pups were inoculated with a heat-killed serotype 23F resident strain (10 5 CFU) for 6h before introduction of the live isogenic challenger strain with increasing CFU (10 1 -10 3 ). Nasal lavages were cultured 3 days later to determine challenger colonization densities, n=3–4. Median values are shown. b, Pups were inoculated with live (10 3 CFU) or heat-killed (10 5 CFU) resident strain for 6h and nasal lavages with RLT lysis buffer were obtained to isolate host RNA. Early inflammatory cytokine expression levels were assessed by SYBR green real-time quantitative RT-PCR. Cytokine expression induced by heat-killed S. pneumoniae were shown as fold change relative to that elicited by live bacteria, n=5–6. Data are shown as mean ± SEM. c-d, Pups were inoculated with either a streptomycin sensitive ( c ) or resistant ( d ) resident strain for 3 days before intranasal administration of streptomycin. An isogenic streptomycin resistant challenger was inoculated 6h later and its colonization density determined after 3 days. Median values are shown. Colonization levels by 10 1 CFU challenger alone, and in resident-colonized pups treated with either phosphate buffered saline (PBS) or streptomycin, were compared by Kruskal–Wallis one-way analysis of variance in ( d ), n=4–8, * p

    Journal: Nature microbiology

    Article Title: Pneumococcal quorum sensing drives an asymmetric owner-intruder competitive strategy during carriage via the competence regulon

    doi: 10.1038/s41564-018-0314-4

    Figure Lengend Snippet: Asymmetric competition is dependent on direct interactions between resident and challenger pneumococci. a, Day 4–5 old pups were inoculated with a heat-killed serotype 23F resident strain (10 5 CFU) for 6h before introduction of the live isogenic challenger strain with increasing CFU (10 1 -10 3 ). Nasal lavages were cultured 3 days later to determine challenger colonization densities, n=3–4. Median values are shown. b, Pups were inoculated with live (10 3 CFU) or heat-killed (10 5 CFU) resident strain for 6h and nasal lavages with RLT lysis buffer were obtained to isolate host RNA. Early inflammatory cytokine expression levels were assessed by SYBR green real-time quantitative RT-PCR. Cytokine expression induced by heat-killed S. pneumoniae were shown as fold change relative to that elicited by live bacteria, n=5–6. Data are shown as mean ± SEM. c-d, Pups were inoculated with either a streptomycin sensitive ( c ) or resistant ( d ) resident strain for 3 days before intranasal administration of streptomycin. An isogenic streptomycin resistant challenger was inoculated 6h later and its colonization density determined after 3 days. Median values are shown. Colonization levels by 10 1 CFU challenger alone, and in resident-colonized pups treated with either phosphate buffered saline (PBS) or streptomycin, were compared by Kruskal–Wallis one-way analysis of variance in ( d ), n=4–8, * p

    Article Snippet: To obtain host RNA, nasal lavages with RLT lysis buffer (Qiagen) were performed and RNA isolated with the RNeasy minikit (Qiagen) as previously described . cDNA was reverse transcribed using a high-capacity cDNA kit (Applied Biosystems) following the manufacturer’s instructions.

    Techniques: Cell Culture, Lysis, Expressing, SYBR Green Assay, Quantitative RT-PCR

    Flow cytometry for isolation of microglia from early adolescent mouse visual cortex. (A) Schematic illustrating the use of fluorescence-activated cell sorting (FACS) to isolate microglia from P28 bilateral V1b. Gating scheme that was used for all samples shows (B) selection of the population containing cells, including microglia; (C) selection of singlets; (D) selection of the propidium iodide (PI) negative population representing live cells; and (E) selection of CD11b+ CD45low microglia that were collected directly into Buffer RLT Plus ™ prior to RNA extraction.

    Journal: Brain, behavior, and immunity

    Article Title: Developmental alcohol exposure impairs synaptic plasticity without overtly altering microglial function in mouse visual cortex

    doi: 10.1016/j.bbi.2017.09.003

    Figure Lengend Snippet: Flow cytometry for isolation of microglia from early adolescent mouse visual cortex. (A) Schematic illustrating the use of fluorescence-activated cell sorting (FACS) to isolate microglia from P28 bilateral V1b. Gating scheme that was used for all samples shows (B) selection of the population containing cells, including microglia; (C) selection of singlets; (D) selection of the propidium iodide (PI) negative population representing live cells; and (E) selection of CD11b+ CD45low microglia that were collected directly into Buffer RLT Plus ™ prior to RNA extraction.

    Article Snippet: For immediate cell lysis and preservation of RNA, microglia (CD11b+, CD45 low) were sorted directly into 1.5mL microcentrifuge tubes (Axygen 311-08-051) containing 600μL Buffer RLT PLUS (Qiagen, 1053393) and 6μL β-mercaptoethanol at 4°C, prepared just prior to sorting.

    Techniques: Flow Cytometry, Cytometry, Isolation, Fluorescence, FACS, Selection, RNA Extraction

    Kinetics of changes in the transcription of purinergic genes, cytokines, and transcription factors following activation of cultured human iNKT cells. Cells were collected in RLT lysis buffer (Qiagen) at various times after activation by TCR engagement (5 μg/ml plate-bound αCD3 Ab) and transcripts were quantified by RT-PCR. The results are representative of duplicate experiments. Gene names and corresponding proteins are: ADORA2A , adenosine A2A receptor; CD38 , cyclic ADP-ribose hydrolase 1/NAD + nucleosidase; CD39 , ectonucleoside triphosphate diphosphohydrolase 1; ENPP1 , ectonucleotide pyrophosphatase/phosphodiesterase 1; CD73 , ecto-5-nucleotidase; P2RX7 , P2X7 receptor; PANX1 , pannexin 1; ENT1 , equilibrative nucleoside transporter 1; ADA ( ADA1 ), adenosine deaminase; CD69 , early lymphocyte activation antigen; IFNG , interferon γ; TBX21 ( TBET ), T-box 21; GATA3 , GATA binding protein 3.

    Journal: JCI Insight

    Article Title: Induction of antiinflammatory purinergic signaling in activated human iNKT cells

    doi: 10.1172/jci.insight.91954

    Figure Lengend Snippet: Kinetics of changes in the transcription of purinergic genes, cytokines, and transcription factors following activation of cultured human iNKT cells. Cells were collected in RLT lysis buffer (Qiagen) at various times after activation by TCR engagement (5 μg/ml plate-bound αCD3 Ab) and transcripts were quantified by RT-PCR. The results are representative of duplicate experiments. Gene names and corresponding proteins are: ADORA2A , adenosine A2A receptor; CD38 , cyclic ADP-ribose hydrolase 1/NAD + nucleosidase; CD39 , ectonucleoside triphosphate diphosphohydrolase 1; ENPP1 , ectonucleotide pyrophosphatase/phosphodiesterase 1; CD73 , ecto-5-nucleotidase; P2RX7 , P2X7 receptor; PANX1 , pannexin 1; ENT1 , equilibrative nucleoside transporter 1; ADA ( ADA1 ), adenosine deaminase; CD69 , early lymphocyte activation antigen; IFNG , interferon γ; TBX21 ( TBET ), T-box 21; GATA3 , GATA binding protein 3.

    Article Snippet: Cultured human iNKT cells were harvested, lysed with RLT Plus lysis buffer (Biolegend), and RNA was extracted using Qiagen Allprep DNA/RNA Micro columns as described by the manufacturer. cDNA was synthesized from RNA samples using Qiagen QuantiTect Reverse Transcription Kits according to the manufacturer’s protocol.

    Techniques: Activation Assay, Cell Culture, Lysis, Reverse Transcription Polymerase Chain Reaction, Binding Assay